Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008732

Physical Location & Seq

Genomic Coordinates Forward (+)

8677049 .. 8682344

Gene Structure 5296 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G25150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2010 bp

ATGGATCCAGAGCAAATCAACGAGTTCGTCGTGGGCTACTTGAAGAAGAAAGGATTCAGTAGCGCTGCTAAAGACCTTGAATCTTATCATCACCAGAACAATAATGGTAGCTCCTTCACTTCTGTCGATTATCATAACGATCCTGAGCTCACGAAGCTCATTCGCTCCTTTTCTCAACAAGAAGATGATCCAACTCGGTACCGTGAAGGTTACAGCAAACTAAGGTCATGGGCGTATAATTCTCTGGATTTATATAAGCATGAATTGCTTCGAGTGATGTATCCTGTGTTTATTCATTGTTACATGGATCTGGTGGGGAAAGGGCACACTCAAGAAGCTAGAGCCTTCTTTAACAGCTTCCGCAAAGATCATGAGATGGTACACTTACGGGATCTTCAGAAGCTTGAAGGCGTGCTTTCTCCATCTCATCTAGAGGAGATGGAGTTTGCTCGTTCTCTTAGAAAAAGCAAGGTTAACATCAAGTTTTGTCAGTATTCATATGAGCTGTTACTTCAGTATCTACATAGTACAGTATCGACTTTAATGCTTGGTATTATTAACGAGCACATCAATTTCCAAGTCTACTCGGGACAGCCTACTTCCTCATCCGATGACATAGAAGCTGTCACCATTGTCGGGAGTTTTCAGGACACTGCTAATCACATTAATCAAAAAGAAATACAGTGGGGATTGCTTGAAGATTCACTAGAAGATCGCCTTGAGAAGACAGGAGGTCTGCTGTCTGATTCTGAGAAGGGACAAGGAGAAAGTAAAGACGGGGATGCAGATGATAGTAAGAAAAGATCCACTGAAATTGGAAAGCAAGGCTCTTCCTTAAAAAAGTTGAAGAAGGACAAAGCGGGTAATGCAACAGCAAAAGTTGCACGTCTGGAGACCATTACGGTGTCACCGGCGCCGCGTGTAAAACCAGAACTTGCCTTGCCAGTAATGTCCACAGATGTAGAGCAATCCATCCTTGAAGACCTTAGAAACCGTGTGCAGTTGAGTAGTGTTGCAATGCCATCAGTCAGCTTTTATACATTTGTGAATACTCATAACGGGTTAAACTGTTCATCTATATCTCATGATGGATCTCTGGTTGCTGGTGGGTTCTCAGATTCTTCAATAAAGGTCTGGGACATGGCTAAGATAGGACAAGCTGGCAGTGGTGCTTTGCAGGCTGAAAATGATTCAAGTGATCAAAGCATAGGACCAAATGGCAGAAGAAGTTATACATTATTACTGGGTCATTCAGGGCCAGTTTACTCAGCCACTTTCAGTCCCCCTGGAGATTTTGTTCTATCTTCTTCAGCAGATACAACAATACGACTGTGGAGCACAAAACTAAATGCTAATCTCGTGTGCTACAAGGGTCATAATTACCCTGTATGGGATGCCCAGTTTAGCCCTTTCGGTCATTACTTTGCAAGCTGTTCTCATGATCGTACTGCACGGATTTGGTCAATGGACCGAATACAACCTCTTCGGATAATGGCAGGGCATCTATCTGATGTTGATTGTGTACAGTGGCATCCCAATTGTAACTACATTGCTACCGGTAGTAGTGATAAGACGGTTAGACTCTGGGATGTGCAAACTGGAGAATGTGTCCGGATATTCATTGGGCACAGGAGTATGGTTTTATCATTGGCGATGTCTCCAGATGGGAGATATATGGCTTCCGGTGATGAAGATGGCACTATAATGATGTGGGACCTCTCAACTGCTCGCTGCATCACTCCTCTCATGGGCCATAATTCATGTGTCTGGAGTCTCTCATACAGCGGGGAGGGCTCTCTTCTGGCTTCTGGATCCGCAGACTGCACAGTGAAACTGTGGGACGTCACTTCAAGTACCAAATTGACAAAGGCCGAGGAAAAGAATGGAAACTCGAACAGATTGCGCTCACTCAGGACTTTCCCCACCAAGTCCACACCAGTTCACGCTCTCCGGTTTTCTCGGAGAAATTTGTTGTTTGCTGCAGGAGCGATCTCTAAACCCGCAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000123 GO:0000228 GO:0000428 GO:0000785 GO:0000790 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005694 GO:0005856 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0015629 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030880 GO:0031248 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0033276 GO:0034641 GO:0034645 GO:0034654 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044798 GO:0046483 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070461 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902493 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko05168 map03022 map05168

Pfam Domains

Protein Families

TFIID_NTD2 WD40

Protein Analysis

669

Amino Acids

74.41

Weight (kDa)

6.65

Isoelectric Point (pI)

46.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIID_NTD2	PF04494	61 - 190	4.4e-45	WD40 associated region in TFIID subunit, NTD2 domain
Beta-prop_WDR5	PF25175	347 - 463	2.7e-10	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	348 - 531	5.9e-26	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	353 - 490	1.7e-10	CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	408 - 550	8.7e-14	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	411 - 530	2.9e-18	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	411 - 578	9.5e-36	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	413 - 482	3.1e-06	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	413 - 491	4.7e-08	EIF3I
WD40	PF00400	414 - 446	5.1e-08	WD domain, G-beta repeat
WD40_Gbeta	PF25391	414 - 530	1.6e-12	G protein beta WD-40 repeat protein
Beta-prop_IFT122_1st	PF23381	414 - 535	2.9e-06	IFT122 first beta-propeller
Beta-prop_THOC3	PF25174	414 - 488	7.1e-13	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	415 - 579	2.6e-44	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	417 - 492	6.2e-10	WDHD1 first WD40 domain
WD40	PF00400	455 - 488	1.2e-08	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	456 - 537	1e-11	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	456 - 538	4e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	457 - 623	2.4e-47	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	464 - 551	1.2e-08	MABP1/WDR62 second WD40 domain
WDR90_beta-prop_4th	PF23342	465 - 576	1.9e-09	WDR90, 4th beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	467 - 622	5e-13	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	468 - 538	2.8e-18	THOC3 beta-propeller domain
Beta-prop_WDR19_1st	PF23389	472 - 621	4.7e-09	WDR19 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	475 - 577	3.4e-14	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	481 - 562	1.3e-10	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	493 - 530	5.9e-11	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	495 - 579	3.8e-11	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	496 - 621	9.7e-21	G protein beta WD-40 repeat protein
WDR55	PF24796	498 - 630	7.4e-14	WDR55
Beta-prop_EML	PF23409	499 - 558	4.9e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_IFT140_1st	PF23383	503 - 573	1.5e-08	IFT140 first beta-propeller
WD40_WDHD1_1st	PF24817	503 - 579	5.7e-15	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	508 - 631	9.8e-18	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	514 - 617	1.1e-19	WDR3 second beta-propeller domain
Beta-prop_SCAP	PF24017	517 - 623	3.2e-08	SCAP Beta-propeller
Beta-prop_WDR3_1st	PF25173	526 - 630	1.2e-26	WDR3 first beta-propeller domain
WD40	PF00400	534 - 572	6.6e-11	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	536 - 627	6.2e-20	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	537 - 623	3.7e-10	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	538 - 624	1.1e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	541 - 622	4.5e-20	WDR5 beta-propeller domain
Beta-prop_DCAF4	PF23761	543 - 621	3.7e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_EML	PF23409	545 - 628	2e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	545 - 624	1e-06	CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	547 - 623	1.2e-09	MABP1/WDR62 second WD40 domain
WD40_Prp19	PF24814	554 - 627	5e-14	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	564 - 619	7.1e-07	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	577 - 614	4.6e-10	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008732)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G25150 AT5G25150
fragaria_vesca	FvH4_2g41020 FvH4_2g41020 FvH4_2g41020 FvH4_2g41020 FvH4_2g41020
malus_domestica	MD00G1143300.v1.1 MD15G1039100.v1.1
prunus_persica	Prupe.1G392800_v2.0.a1 Prupe.1G392800_v2.0.a1
pyrus_communis	pycom08g04780
rosa_chinensis	RchiOBHm_Chr6g0299541
rosa_laevigata	RLG00000011417
rosa_multiflora	Rmu_sc0004064.1_g000008
rosa_roxburghii	Rroxscaffold_7G00168570
rosa_rugosa	Rorug06G0289700 Rorug06G0289800 Rorug06G0289900 Rorug06G0289900
rosa_samantha	Rh6AG401100 Rh6DG402200
rosa_wichuraiana	Rw6G035100

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1845
Acc65I	GGTACC	1 cut(s)	198
AccB1I	GGYRCC	2 cut(s)	198, 913
AccI	GTMKAC	1 cut(s)	582
AccII	CGCG	1 cut(s)	919
AccIII	TCCGGA	1 cut(s)	1611
AciI	CCGC	6 cut(s)	361, 860, 917, 1785, 1815, 2001
AclWI	GGATC	9 cut(s)	12, 134, 182, 315, 399, 798, 1099, 1806, 1819
AcsI	RAATTY	1 cut(s)	1966
AcuI	CTGAAG	3 cut(s)	380, 497, 1293
AcyI	GRCGYC	2 cut(s)	914, 1842
AfaI	GTAC	6 cut(s)	200, 381, 529, 1447, 1524, 1855
AfeI	AGCGCT	1 cut(s)	64
AfiI	CCNNNNNNNGG	3 cut(s)	636, 1390, 1748
AgeI	ACCGGT	1 cut(s)	1556
AgsI	TTSAA	9 cut(s)	43, 80, 407, 698, 847, 980, 1125, 1194, 1851
AhdI	GACNNNNNGTC	1 cut(s)	732
AjiI	CACGTC	1 cut(s)	887
AjnI	CCWGG	1 cut(s)	1285
AloI	GAACNNNNNNTCC	2 cut(s)	1281, 1313
Alw21I	GWGCWC	3 cut(s)	150, 567, 1340
Alw26I	GTCTC	3 cut(s)	887, 1662, 1778
AlwI	GGATC	9 cut(s)	12, 134, 182, 315, 399, 798, 1099, 1806, 1819
Ama87I	CYCGRG	1 cut(s)	586
Aor13HI	TCCGGA	1 cut(s)	1611
Aor51HI	AGCGCT	1 cut(s)	64
AoxI	GGCC	3 cut(s)	1256, 1750, 1869
ApeKI	GCWGC	3 cut(s)	65, 1731, 1979
ApoI	RAATTY	1 cut(s)	1966
AseI	ATTAAT	1 cut(s)	666
AsiGI	ACCGGT	1 cut(s)	1556
Asp700I	GAANNNNTTC	2 cut(s)	53, 267
Asp718I	GGTACC	1 cut(s)	198
AspLEI	GCGC	3 cut(s)	65, 916, 1905
AspS9I	GGNCC	5 cut(s)	1211, 1256, 1468, 1714, 1750
AsuHPI	GGTGA	4 cut(s)	83, 619, 900, 1697
AvaI	CYCGRG	1 cut(s)	586
AvaII	GGWCC	3 cut(s)	1211, 1468, 1714
BaeGI	GKGCMC	2 cut(s)	327, 1629
BaeI	ACNNNNGTAYC	2 cut(s)	500, 533
BamHI	GGATCC	2 cut(s)	4, 1811
BanI	GGYRCC	2 cut(s)	198, 913
BanII	GRGCYC	2 cut(s)	150, 1796
BarI	GAAGNNNNNNTAC	2 cut(s)	1216, 1248
BauI	CACGAG	1 cut(s)	1358
BbsI	GAAGAC	2 cut(s)	731, 987
Bbv12I	GWGCWC	3 cut(s)	150, 567, 1340
BbvI	GCAGC	3 cut(s)	52, 1718, 1966
BccI	CCATC	8 cut(s)	370, 430, 433, 980, 1030, 1082, 1658, 1688
BcgI	CGANNNNNNTGC	4 cut(s)	526, 560, 1882, 1916
BciT130I	CCWGG	1 cut(s)	1287
BciVI	GTATCC	1 cut(s)	291
BclI	TGATCA	1 cut(s)	1198
BcoDI	GTCTC	3 cut(s)	887, 1662, 1778
BfaI	CTAG	3 cut(s)	339, 431, 707
BfmI	CTRYAG	1 cut(s)	1980
BfoI	RGCGCY	2 cut(s)	66, 917
BfuI	GTATCC	1 cut(s)	291
BisI	GCNGC	4 cut(s)	66, 917, 1732, 1980
BlsI	GCNGC	4 cut(s)	67, 918, 1733, 1981
Bme1390I	CCNGG	1 cut(s)	1287
Bme18I	GGWCC	3 cut(s)	1211, 1468, 1714
BmeRI	GACNNNNNGTC	1 cut(s)	732
BmeT110I	CYCGRG	1 cut(s)	586
BmgBI	CACGTC	1 cut(s)	887
BmgT120I	GGNCC	5 cut(s)	1211, 1256, 1468, 1714, 1750
BmiI	GGNNCC	5 cut(s)	6, 200, 915, 1715, 1813
BmrFI	CCNGG	1 cut(s)	1287
BmrI	ACTGGG	2 cut(s)	1253, 1393
BmsI	GCATC	5 cut(s)	772, 1384, 1510, 1540, 1743
BmuI	ACTGGG	2 cut(s)	1253, 1393
BpiI	GAAGAC	2 cut(s)	731, 987
BpmI	CTGGAG	5 cut(s)	911, 1308, 1620, 1644, 1789
Bpu10I	CCTNAGC	1 cut(s)	144
BpuEI	CTTGAG	2 cut(s)	315, 740
BsaHI	GRCGYC	2 cut(s)	914, 1842
BsaI	GGTCTC	1 cut(s)	887
BsaJI	CCNNGG	2 cut(s)	1285, 1872
BsaWI	WCCGGW	4 cut(s)	1556, 1611, 1682, 1950
BsaXI	ACNNNNNCTCC	6 cut(s)	428, 458, 1281, 1311, 1593, 1623
Bsc4I	CCNNNNNNNGG	3 cut(s)	636, 1390, 1748
Bse118I	RCCGGY	2 cut(s)	910, 1556
Bse1I	ACTGG	6 cut(s)	944, 1248, 1259, 1399, 1603, 1937
Bse3DI	GCAATG	2 cut(s)	1023, 1548
BseAI	TCCGGA	1 cut(s)	1611
BseBI	CCWGG	1 cut(s)	1287
BseDI	CCNNGG	2 cut(s)	1285, 1872
BseGI	GGATG	6 cut(s)	605, 787, 972, 1399, 1531, 1594
BseLI	CCNNNNNNNGG	3 cut(s)	636, 1390, 1748
BseMI	GCAATG	2 cut(s)	1023, 1548
BseMII	CTCAG	5 cut(s)	135, 741, 1128, 1281, 1924
BseNI	ACTGG	6 cut(s)	944, 1248, 1259, 1399, 1603, 1937
BseRI	GAGGAG	2 cut(s)	449, 1731
BseSI	GKGCMC	2 cut(s)	327, 1629
BseXI	GCAGC	3 cut(s)	52, 1718, 1966
BsgI	GTGCAG	3 cut(s)	1019, 1434, 1807
Bsh1236I	CGCG	1 cut(s)	919
BshFI	GGCC	3 cut(s)	1258, 1752, 1871
BshNI	GGYRCC	2 cut(s)	198, 913
BshTI	ACCGGT	1 cut(s)	1556
BsiHKAI	GWGCWC	3 cut(s)	150, 567, 1340
BsiHKCI	CYCGRG	1 cut(s)	586
BsiSI	CCGG	5 cut(s)	911, 1557, 1612, 1683, 1951
BslFI	GGGAC	6 cut(s)	603, 771, 1151, 1266, 1727, 1853
BslI	CCNNNNNNNGG	3 cut(s)	636, 1390, 1748
BsmAI	GTCTC	3 cut(s)	887, 1662, 1778
BsmFI	GGGAC	6 cut(s)	603, 771, 1151, 1266, 1727, 1853
BsnI	GGCC	3 cut(s)	1258, 1752, 1871
Bso31I	GGTCTC	1 cut(s)	887
BsoBI	CYCGRG	1 cut(s)	586
Bsp1286I	GDGCHC	6 cut(s)	150, 327, 567, 1340, 1629, 1796
Bsp13I	TCCGGA	1 cut(s)	1611
Bsp1407I	TGTACA	1 cut(s)	1522
BspACI	CCGC	6 cut(s)	361, 860, 917, 1785, 1815, 2001
BspANI	GGCC	3 cut(s)	1258, 1752, 1871
BspCNI	CTCAG	5 cut(s)	136, 742, 1127, 1280, 1923
BspEI	TCCGGA	1 cut(s)	1611
BspFNI	CGCG	1 cut(s)	919
BspHI	TCATGA	3 cut(s)	370, 1084, 1438
BspLI	GGNNCC	5 cut(s)	6, 200, 915, 1715, 1813
BspMAI	CTGCAG	1 cut(s)	1984
BspPI	GGATC	9 cut(s)	12, 134, 182, 315, 399, 798, 1099, 1806, 1819
BspQI	GCTCTTC	1 cut(s)	835
BspT107I	GGYRCC	2 cut(s)	198, 913
BspTNI	GGTCTC	1 cut(s)	887
BsrDI	GCAATG	2 cut(s)	1023, 1548
BsrFI	RCCGGY	2 cut(s)	910, 1556
BsrGI	TGTACA	1 cut(s)	1522
BsrI	ACTGG	6 cut(s)	944, 1248, 1259, 1399, 1603, 1937
BssAI	RCCGGY	2 cut(s)	910, 1556
BssECI	CCNNGG	2 cut(s)	1285, 1872
BssNI	GRCGYC	2 cut(s)	914, 1842
BssSI	CACGAG	1 cut(s)	1358
Bst2BI	CACGAG	1 cut(s)	1358
Bst2UI	CCWGG	1 cut(s)	1287
Bst6I	CTCTTC	3 cut(s)	835, 1488, 1803
BstACI	GRCGYC	2 cut(s)	914, 1842
BstAPI	GCANNNNNTGC	2 cut(s)	265, 881
BstAUI	TGTACA	1 cut(s)	1522
BstC8I	GCNNGC	5 cut(s)	413, 1162, 1179, 1429, 1729
BstDEI	CTNAG	9 cut(s)	144, 221, 458, 750, 986, 1114, 1146, 1267, 1910
BstF5I	GGATG	6 cut(s)	605, 787, 972, 1399, 1531, 1594
BstFNI	CGCG	1 cut(s)	919
BstH2I	RGCGCY	2 cut(s)	66, 917
BstHHI	GCGC	3 cut(s)	65, 916, 1905
BstMAI	GTCTC	3 cut(s)	887, 1662, 1778
BstMWI	GCNNNNNNNGC	7 cut(s)	154, 265, 866, 881, 1178, 1791, 1985
BstNI	CCWGG	1 cut(s)	1287
BstSCI	CCNGG	1 cut(s)	1285
BstSFI	CTRYAG	1 cut(s)	1980
BstSLI	GKGCMC	2 cut(s)	327, 1629
BstUI	CGCG	1 cut(s)	919
BstV1I	GCAGC	3 cut(s)	52, 1718, 1966
BstV2I	GAAGAC	2 cut(s)	731, 987
BstX2I	RGATCY	6 cut(s)	4, 307, 391, 803, 1091, 1811
BstYI	RGATCY	6 cut(s)	4, 307, 391, 803, 1091, 1811
BsuI	GTATCC	1 cut(s)	291
BsuRI	GGCC	3 cut(s)	1258, 1752, 1871
BtgZI	GCGATG	1 cut(s)	1667
BtrI	CACGTC	1 cut(s)	887
BtsCI	GGATG	6 cut(s)	605, 787, 972, 1399, 1531, 1594
BtsI	GCAGTG	2 cut(s)	651, 1171
BtsIMutI	CAGTG	6 cut(s)	651, 689, 807, 1171, 1532, 1833
Cac8I	GCNNGC	5 cut(s)	413, 1162, 1179, 1429, 1729
CciI	TCATGA	3 cut(s)	370, 1084, 1438
CfoI	GCGC	3 cut(s)	65, 916, 1905
Cfr10I	RCCGGY	2 cut(s)	910, 1556
Cfr13I	GGNCC	5 cut(s)	1211, 1256, 1468, 1714, 1750
Csp6I	GTAC	6 cut(s)	199, 380, 528, 1446, 1523, 1854
CspAI	ACCGGT	1 cut(s)	1556
CspCI	CAANNNNNGTGG	2 cut(s)	796, 831
CviAII	CATG	9 cut(s)	228, 260, 304, 371, 1085, 1141, 1439, 1747, 1761
CviQI	GTAC	6 cut(s)	199, 380, 528, 1446, 1523, 1854
DdeI	CTNAG	9 cut(s)	144, 221, 458, 750, 986, 1114, 1146, 1267, 1910
DinI	GGCGCC	1 cut(s)	915
DriI	GACNNNNNGTC	1 cut(s)	732
Eam1104I	CTCTTC	3 cut(s)	835, 1488, 1803
Eam1105I	GACNNNNNGTC	1 cut(s)	732
EarI	CTCTTC	3 cut(s)	835, 1488, 1803
Ecl136II	GAGCTC	1 cut(s)	148
Eco24I	GRGCYC	2 cut(s)	150, 1796
Eco31I	GGTCTC	1 cut(s)	887
Eco47I	GGWCC	3 cut(s)	1211, 1468, 1714
Eco47III	AGCGCT	1 cut(s)	64
Eco53kI	GAGCTC	1 cut(s)	148
Eco57I	CTGAAG	3 cut(s)	380, 497, 1293
Eco88I	CYCGRG	1 cut(s)	586
EcoICRI	GAGCTC	1 cut(s)	148
EcoO109I	RGGNCCY	1 cut(s)	1714
EcoRII	CCWGG	1 cut(s)	1285
EcoT38I	GRGCYC	2 cut(s)	150, 1796
EgeI	GGCGCC	1 cut(s)	915
EheI	GGCGCC	1 cut(s)	915
FaeI	CATG	9 cut(s)	231, 263, 307, 374, 1088, 1144, 1442, 1750, 1764
FalI	AAGNNNNNCTT	4 cut(s)	399, 431, 702, 734
FaqI	GGGAC	6 cut(s)	603, 771, 1151, 1266, 1727, 1853
FatI	CATG	9 cut(s)	227, 259, 303, 370, 1084, 1140, 1438, 1746, 1760
FauI	CCCGC	3 cut(s)	853, 1778, 2008
FauNDI	CATATG	1 cut(s)	499
FbaI	TGATCA	1 cut(s)	1198
FblI	GTMKAC	1 cut(s)	582
Fnu4HI	GCNGC	4 cut(s)	66, 917, 1732, 1980
FokI	GGATG	6 cut(s)	592, 794, 959, 1406, 1518, 1601
FriOI	GRGCYC	2 cut(s)	150, 1796
Fsp4HI	GCNGC	4 cut(s)	66, 917, 1732, 1980
FspBI	CTAG	3 cut(s)	339, 431, 707
GlaI	GCGC	3 cut(s)	64, 915, 1904
GluI	GCNGC	4 cut(s)	66, 917, 1732, 1980
GsuI	CTGGAG	5 cut(s)	911, 1308, 1620, 1644, 1789
HaeII	RGCGCY	2 cut(s)	66, 917
HaeIII	GGCC	3 cut(s)	1258, 1752, 1871
HapII	CCGG	5 cut(s)	911, 1557, 1612, 1683, 1951
HhaI	GCGC	3 cut(s)	65, 916, 1905
Hin1I	GRCGYC	2 cut(s)	914, 1842
Hin1II	CATG	9 cut(s)	231, 263, 307, 374, 1088, 1144, 1442, 1750, 1764
Hin6I	GCGC	3 cut(s)	63, 914, 1903
HinP1I	GCGC	3 cut(s)	63, 914, 1903
HincII	GTYRAC	1 cut(s)	475
HindII	GTYRAC	1 cut(s)	475
HindIII	AAGCTT	1 cut(s)	401
HinfI	GANTC	8 cut(s)	54, 80, 701, 746, 1118, 1190, 1581, 1771
HpaI	GTTAAC	1 cut(s)	475
HpaII	CCGG	5 cut(s)	911, 1557, 1612, 1683, 1951
HphI	GGTGA	4 cut(s)	83, 619, 900, 1697
Hpy166II	GTNNAC	8 cut(s)	382, 475, 583, 954, 1264, 1523, 1932, 1942
Hpy188I	TCNGA	8 cut(s)	399, 610, 745, 751, 1117, 1488, 1510, 1962
Hpy8I	GTNNAC	8 cut(s)	382, 475, 583, 954, 1264, 1523, 1932, 1942
Hpy99I	CGWCG	1 cut(s)	32
HpyAV	CCTTC	6 cut(s)	124, 200, 355, 401, 748, 844
HpyCH4IV	ACGT	2 cut(s)	886, 1842
HpyF10VI	GCNNNNNNNGC	7 cut(s)	154, 265, 866, 881, 1178, 1791, 1985
HpyF3I	CTNAG	9 cut(s)	144, 221, 458, 750, 986, 1114, 1146, 1267, 1910
HpySE526I	ACGT	2 cut(s)	886, 1842
Hsp92I	GRCGYC	2 cut(s)	914, 1842
Hsp92II	CATG	9 cut(s)	231, 263, 307, 374, 1088, 1144, 1442, 1750, 1764
HspAI	GCGC	3 cut(s)	63, 914, 1903
KasI	GGCGCC	1 cut(s)	913
Kpn2I	TCCGGA	1 cut(s)	1611
KpnI	GGTACC	1 cut(s)	202
Ksp22I	TGATCA	1 cut(s)	1198
KspAI	GTTAAC	1 cut(s)	475
LguI	GCTCTTC	1 cut(s)	835
LmnI	GCTCC	4 cut(s)	116, 170, 1335, 1985
Lsp1109I	GCAGC	3 cut(s)	52, 1718, 1966
LweI	GCATC	5 cut(s)	772, 1384, 1510, 1540, 1743
MaeI	CTAG	3 cut(s)	339, 431, 707
MaeII	ACGT	2 cut(s)	886, 1842
MaeIII	GTNAC	7 cut(s)	209, 299, 507, 625, 906, 1541, 1843
MfeI	CAATTG	1 cut(s)	1537
MflI	RGATCY	6 cut(s)	4, 307, 391, 803, 1091, 1811
MhlI	GDGCHC	6 cut(s)	150, 327, 567, 1340, 1629, 1796
MluCI	AATT	9 cut(s)	238, 263, 571, 813, 1378, 1537, 1756, 1859, 1966
Mly113I	GGCGCC	1 cut(s)	914
MlyI	GAGTC	2 cut(s)	1575, 1780
MmeI	TCCRAC	1 cut(s)	215
MnlI	CCTC	8 cut(s)	427, 613, 725, 1491, 1727, 1752, 1783, 1867
MroI	TCCGGA	1 cut(s)	1611
MroXI	GAANNNNTTC	2 cut(s)	53, 267
MseI	TTAA	7 cut(s)	351, 474, 542, 558, 666, 836, 1064
MslI	CAYNNNNRTG	2 cut(s)	902, 1703
MspA1I	CMGCKG	1 cut(s)	1785
MspI	CCGG	5 cut(s)	911, 1557, 1612, 1683, 1951
MspR9I	CCNGG	1 cut(s)	1287
MunI	CAATTG	1 cut(s)	1537
MvaI	CCWGG	1 cut(s)	1287
MvnI	CGCG	1 cut(s)	919
MwoI	GCNNNNNNNGC	7 cut(s)	154, 265, 866, 881, 1178, 1791, 1985
NarI	GGCGCC	1 cut(s)	914
NdeI	CATATG	1 cut(s)	499
NlaIII	CATG	9 cut(s)	231, 263, 307, 374, 1088, 1144, 1442, 1750, 1764
NlaIV	GGNNCC	5 cut(s)	6, 200, 915, 1715, 1813
NmeAIII	GCCGAG	1 cut(s)	1897
NmuCI	GTSAC	3 cut(s)	625, 906, 1843
PagI	TCATGA	3 cut(s)	370, 1084, 1438
PciSI	GCTCTTC	1 cut(s)	835
PcsI	WCGNNNNNNNCGW	1 cut(s)	27
PdmI	GAANNNNTTC	2 cut(s)	53, 267
PfeI	GAWTC	6 cut(s)	54, 80, 701, 746, 1118, 1190
PinAI	ACCGGT	1 cut(s)	1556
PkrI	GCNGC	4 cut(s)	67, 918, 1733, 1981
PleI	GAGTC	2 cut(s)	1575, 1779
PluTI	GGCGCC	1 cut(s)	917
PpsI	GAGTC	2 cut(s)	1575, 1779
PpuMI	RGGWCCY	1 cut(s)	1714
PshBI	ATTAAT	1 cut(s)	666
Psp124BI	GAGCTC	1 cut(s)	150
Psp5II	RGGWCCY	1 cut(s)	1714
Psp6I	CCWGG	1 cut(s)	1285
PspGI	CCWGG	1 cut(s)	1285
PspN4I	GGNNCC	5 cut(s)	6, 200, 915, 1715, 1813
PspPI	GGNCC	5 cut(s)	1211, 1256, 1468, 1714, 1750
PspPPI	RGGWCCY	1 cut(s)	1714
PstI	CTGCAG	1 cut(s)	1984
PsuI	RGATCY	6 cut(s)	4, 307, 391, 803, 1091, 1811
RsaI	GTAC	6 cut(s)	200, 381, 529, 1447, 1524, 1855
RsaNI	GTAC	6 cut(s)	199, 380, 528, 1446, 1523, 1854
RseI	CAYNNNNRTG	2 cut(s)	902, 1703
SacI	GAGCTC	1 cut(s)	150
SapI	GCTCTTC	1 cut(s)	835
SaqAI	TTAA	7 cut(s)	351, 474, 542, 558, 666, 836, 1064
SatI	GCNGC	4 cut(s)	66, 917, 1732, 1980
Sau96I	GGNCC	5 cut(s)	1211, 1256, 1468, 1714, 1750
SchI	GAGTC	2 cut(s)	1575, 1780
ScrFI	CCNGG	1 cut(s)	1287
SduI	GDGCHC	6 cut(s)	150, 327, 567, 1340, 1629, 1796
SfaNI	GCATC	5 cut(s)	772, 1384, 1510, 1540, 1743
SfcI	CTRYAG	1 cut(s)	1980
SfoI	GGCGCC	1 cut(s)	915
SgrAI	CRCCGGYG	1 cut(s)	910
SinI	GGWCC	3 cut(s)	1211, 1468, 1714
SmiMI	CAYNNNNRTG	2 cut(s)	902, 1703
SmlI	CTYRAG	2 cut(s)	330, 719
SmoI	CTYRAG	2 cut(s)	330, 719
Sse9I	AATT	9 cut(s)	238, 263, 571, 813, 1378, 1537, 1756, 1859, 1966
SsiI	CCGC	6 cut(s)	361, 860, 917, 1785, 1815, 2001
SspDI	GGCGCC	1 cut(s)	913
SspMI	CTAG	3 cut(s)	339, 431, 707
SstI	GAGCTC	1 cut(s)	150
StyD4I	CCNGG	1 cut(s)	1285
TaiI	ACGT	2 cut(s)	889, 1845
TaqI	TCGA	4 cut(s)	126, 271, 536, 1892
TaqII	GACCGA	2 cut(s)	1403, 1485
TasI	AATT	9 cut(s)	238, 263, 571, 813, 1378, 1537, 1756, 1859, 1966
TatI	WGTACW	2 cut(s)	527, 1522
TauI	GCSGC	1 cut(s)	919
TfiI	GAWTC	6 cut(s)	54, 80, 701, 746, 1118, 1190
Tru1I	TTAA	7 cut(s)	351, 474, 542, 558, 666, 836, 1064
Tru9I	TTAA	7 cut(s)	351, 474, 542, 558, 666, 836, 1064
TscAI	CASTG	6 cut(s)	658, 689, 814, 1171, 1532, 1833
TseFI	GTSAC	3 cut(s)	625, 906, 1843
TseI	GCWGC	3 cut(s)	65, 1731, 1979
Tsp45I	GTSAC	3 cut(s)	625, 906, 1843
TspDTI	ATGAA	7 cut(s)	276, 284, 486, 1062, 1609, 1704, 1749
TspGWI	ACGGA	1 cut(s)	1468
TspRI	CASTG	6 cut(s)	658, 689, 814, 1171, 1532, 1833
VpaK11BI	GGWCC	3 cut(s)	1211, 1468, 1714
VspI	ATTAAT	1 cut(s)	666
XapI	RAATTY	1 cut(s)	1966
XbaI	TCTAGA	1 cut(s)	430
XcmI	CCANNNNNNNNNTGG	1 cut(s)	101
XmiI	GTMKAC	1 cut(s)	582
XmnI	GAANNNNTTC	2 cut(s)	53, 267
XspI	CTAG	3 cut(s)	339, 431, 707
ZraI	GACGTC	1 cut(s)	1843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.