AT5G25320

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011257
Physical Location & Seq
Reverse (-)
8787196 .. 8789846
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25320.3

Sequence Viewer

Length: 1503 bp
ATGCAGAAAGTTTGCTGGCCTTACTTTGATCCTGATTTCGACAATCTTGGTGAACGCATATACGGTCCACCATGCAGGGTCTATATTGACAATGACAGCATTCAAGATTGCACCGTTGTGAAGGTGAATAGTGAGAACAAACAAGGGCTTCTTCTAGAAGTGGTGCAAATCTTGACGGACATGAATCTTATTATCACCAAGAGTTACATCTCTTCCGATGGTGGATGGTTCATGGATGTTTTCCATGTTAAAGATGAGCATGGAAATAAACTCACTGATAAAAGCGTCATCAACCACATTAAACATGCCATTGGTACGAGTAGGCGAGAATCCGATTTTATAAAGGCTAGTGAAGCGAATAACAATTCCTTGGAACCTCAATTGGCTGATCACGGCGAACACACAGCGATAGAGATGACCGGGACAGACCGACCGGGCCTCTTCTCAGAGATATTTGCTGCTTTCGCGGACTTACATTGCAACGTCATGGAAGCTCATGCTTGGAGCCACAACGCTCGTTTGGCTTGCATTGCCTATGTCTCTGACGACAATACTCACACTCCCATTGATGACCCGAGCCGCTTGGCTTCCATTGAGGACCACCTAAGTACTGTGATCCGTGCCACAGCGGATCCCGCCTCCAACTCTACACACGTGGGTCACAAGGAAAACGAGACTGATGGGTTTCTTGCAGGACAAGGCAAAGGGTGTATGAATAGCAACATGGAGAGACGGTTGCATCAGCTAATGTTATCTGTGAGAGACTTTGACGAACCGTTTTGTGAGCCTTCTTCGTTGTCATTATTGTCGTCCAAGTTAGAGTACTGCGATCATAAGGAGAGGAAGACAACTATAGTTTCGATTGGGAATTGCGAAGAAAGAGGATACTCTATTGTCACAGTGAAATCTAAGGATCGAAGAAGGCTCATGTTTGATACAATTTGTACTTTGGTTGATATGCAATACGTTATCTTTCACGCCGCTCTCCGGTCTGATGGAGCTGATGCTTTTCAAGAATACTTCATAAGACACATAGATGGAAGGGCATTGAACACGGAAGGCGAGAAAGAACGGGTGATTAAATGCTTGGAGGCTGCAATAGAACGCCGCGTTTGCGAGGGCGTGAAGTTGGAGTTATGCGCAGAGAACAGAGTAGGCTTACTCTCTGACATCACTCGTGTACTACGTGAGAATGGTTTAACCGTTGTCCGTGCAGACGTGGAGACTCAAGGCCAAAAGTCTCTCAATGCTTTTTACGTCAGAGATATTTCCGGCAACAAAATCGATATGGAGTTTGTGGAGTCAGTGAAGAAAGAGATGAGACCAATCCACCTTGAGGTCAAGAATGAAGACACAAAAATAGACACAGTTGGATCGGATGAACCGACAGCCTCAGCCTCAGCCACACCGCAACGGCAACCACAACCGCACCGTTTCTCGCTTGGTGACATCCTTCGATCACAGATGGAACGCCTTTCTCTCAACTTCGTCCCAACCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

500

Amino Acids

56.31

Weight (kDa)

5.43

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 338 - 383 7.7e-07 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 341
Acc16I TGCGCA 1 cut(s) 1141
AccBSI CCGCTC 1 cut(s) 983
AccII CGCG 2 cut(s) 467, 1110
AciI CCGC 8 cut(s) 467, 580, 629, 636, 981, 1108, 1409, 1427
AclWI GGATC 6 cut(s) 23, 610, 626, 639, 921, 1381
AcvI CACGTG 1 cut(s) 655
AfaI GTAC 5 cut(s) 316, 610, 824, 946, 1182
AfiI CCNNNNNNNGG 2 cut(s) 987, 1336
AflIII ACRYGT 1 cut(s) 652
AgsI TTSAA 3 cut(s) 104, 1013, 1051
AjiI CACGTC 1 cut(s) 1219
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 3 cut(s) 494, 745, 1001
AluI AGCT 3 cut(s) 494, 745, 1001
Alw26I GTCTC 7 cut(s) 544, 668, 724, 756, 1217, 1245, 1315
AlwI GGATC 6 cut(s) 23, 610, 626, 639, 921, 1381
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 3 cut(s) 17, 436, 1231
ApeKI GCWGC 2 cut(s) 458, 1094
AspLEI GCGC 1 cut(s) 1142
AspS9I GGNCC 3 cut(s) 65, 436, 598
AsuC2I CCSGG 2 cut(s) 421, 435
AsuHPI GGTGA 5 cut(s) 62, 136, 187, 1087, 1457
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 2 cut(s) 65, 598
BamHI GGATCC 1 cut(s) 631
BauI CACGAG 1 cut(s) 1176
BbrPI CACGTG 1 cut(s) 655
BbsI GAAGAC 2 cut(s) 851, 1356
BbvCI CCTCAGC 2 cut(s) 1393, 1399
BbvI GCAGC 2 cut(s) 445, 1081
BccI CCATC 6 cut(s) 212, 219, 674, 989, 1031, 1459
BceAI ACGGC 2 cut(s) 409, 1430
BcgI CGANNNNNNTGC 2 cut(s) 1264, 1298
BciVI GTATCC 1 cut(s) 878
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 2 cut(s) 421, 435
BcoDI GTCTC 7 cut(s) 544, 668, 724, 756, 1217, 1245, 1315
BfaI CTAG 2 cut(s) 155, 348
BfmI CTRYAG 1 cut(s) 852
BfuI GTATCC 1 cut(s) 878
BisI GCNGC 5 cut(s) 459, 580, 981, 1095, 1108
BlsI GCNGC 5 cut(s) 460, 581, 982, 1096, 1109
BmcAI AGTACT 2 cut(s) 610, 824
Bme1390I CCNGG 2 cut(s) 421, 435
Bme18I GGWCC 2 cut(s) 65, 598
BmeT110I CYCGRG 1 cut(s) 574
BmgBI CACGTC 1 cut(s) 1219
BmgT120I GGNCC 3 cut(s) 65, 436, 598
BmiI GGNNCC 3 cut(s) 375, 506, 633
BmrFI CCNGG 2 cut(s) 421, 435
BmsI GCATC 2 cut(s) 748, 994
BpiI GAAGAC 2 cut(s) 851, 1356
Bpu10I CCTNAGC 2 cut(s) 1393, 1399
BpuEI CTTGAG 2 cut(s) 1212, 1355
BpuMI CCSGG 2 cut(s) 421, 435
Bsa29I ATCGAT 1 cut(s) 1284
BsaAI YACGTR 2 cut(s) 655, 1187
BsaI GGTCTC 1 cut(s) 1315
BsaJI CCNNGG 1 cut(s) 369
BsaWI WCCGGW 1 cut(s) 987
BsaXI ACNNNNNCTCC 4 cut(s) 544, 574, 719, 749
Bsc4I CCNNNNNNNGG 2 cut(s) 987, 1336
Bse3DI GCAATG 2 cut(s) 475, 528
BseCI ATCGAT 1 cut(s) 1284
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 4 cut(s) 230, 241, 1384, 1449
BseLI CCNNNNNNNGG 2 cut(s) 987, 1336
BseMI GCAATG 2 cut(s) 475, 528
BseMII CTCAG 3 cut(s) 459, 1407, 1413
BseXI GCAGC 2 cut(s) 445, 1081
BsgI GTGCAG 1 cut(s) 1233
Bsh1236I CGCG 2 cut(s) 467, 1110
Bsh1285I CGRYCG 1 cut(s) 434
BshFI GGCC 3 cut(s) 19, 438, 1233
BshVI ATCGAT 1 cut(s) 1284
BsiEI CGRYCG 1 cut(s) 434
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 4 cut(s) 420, 434, 988, 1272
BslFI GGGAC 2 cut(s) 436, 1475
BslI CCNNNNNNNGG 2 cut(s) 987, 1336
BsmAI GTCTC 7 cut(s) 544, 668, 724, 756, 1217, 1245, 1315
BsmBI CGTCTC 1 cut(s) 724
BsmFI GGGAC 2 cut(s) 436, 1475
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 3 cut(s) 19, 438, 1233
Bso31I GGTCTC 1 cut(s) 1315
BsoBI CYCGRG 1 cut(s) 574
Bsp143I GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
BspACI CCGC 8 cut(s) 467, 580, 629, 636, 981, 1108, 1409, 1427
BspANI GGCC 3 cut(s) 19, 438, 1233
BspCNI CTCAG 3 cut(s) 458, 1406, 1412
BspDI ATCGAT 1 cut(s) 1284
BspFNI CGCG 2 cut(s) 467, 1110
BspLI GGNNCC 3 cut(s) 375, 506, 633
BspPI GGATC 6 cut(s) 23, 610, 626, 639, 921, 1381
BspTNI GGTCTC 1 cut(s) 1315
BsrBI CCGCTC 1 cut(s) 983
BsrDI GCAATG 2 cut(s) 475, 528
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
BssSI CACGAG 1 cut(s) 1176
BssT1I CCWWGG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 1176
Bst4CI ACNGT 9 cut(s) 65, 115, 613, 735, 777, 901, 1204, 1369, 1433
Bst6I CTCTTC 2 cut(s) 217, 446
BstBAI YACGTR 2 cut(s) 655, 1187
BstC8I GCNNGC 2 cut(s) 17, 526
BstDEI CTNAG 5 cut(s) 445, 605, 909, 1393, 1399
BstF5I GGATG 4 cut(s) 230, 241, 1384, 1449
BstFNI CGCG 2 cut(s) 467, 1110
BstHHI GCGC 1 cut(s) 1142
BstKTI GATC 8 cut(s) 31, 391, 618, 634, 832, 916, 1376, 1460
BstMAI GTCTC 7 cut(s) 544, 668, 724, 756, 1217, 1245, 1315
BstMBI GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
BstMCI CGRYCG 1 cut(s) 434
BstMWI GCNNNNNNNGC 6 cut(s) 353, 464, 521, 530, 635, 1113
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 2 cut(s) 419, 433
BstSFI CTRYAG 1 cut(s) 852
BstUI CGCG 2 cut(s) 467, 1110
BstV1I GCAGC 2 cut(s) 445, 1081
BstV2I GAAGAC 2 cut(s) 851, 1356
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
Bsu15I ATCGAT 1 cut(s) 1284
BsuI GTATCC 1 cut(s) 878
BsuRI GGCC 3 cut(s) 19, 438, 1233
BsuTUI ATCGAT 1 cut(s) 1284
BtrI CACGTC 1 cut(s) 1219
BtsCI GGATG 4 cut(s) 230, 241, 1384, 1449
BtsIMutI CAGTG 3 cut(s) 273, 906, 1311
Cac8I GCNNGC 2 cut(s) 17, 526
CfoI GCGC 1 cut(s) 1142
Cfr13I GGNCC 3 cut(s) 65, 436, 598
ClaI ATCGAT 1 cut(s) 1284
CseI GACGC 1 cut(s) 274
Csp6I GTAC 5 cut(s) 315, 609, 823, 945, 1181
CviQI GTAC 5 cut(s) 315, 609, 823, 945, 1181
DdeI CTNAG 5 cut(s) 445, 605, 909, 1393, 1399
DpnI GATC 8 cut(s) 30, 390, 617, 633, 831, 915, 1375, 1459
DpnII GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
Eam1104I CTCTTC 2 cut(s) 217, 446
EarI CTCTTC 2 cut(s) 217, 446
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 1315
Eco47I GGWCC 2 cut(s) 65, 598
Eco72I CACGTG 1 cut(s) 655
Eco88I CYCGRG 1 cut(s) 574
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 724
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 2 cut(s) 436, 1475
FauI CCCGC 1 cut(s) 643
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 5 cut(s) 459, 580, 981, 1095, 1108
FokI GGATG 4 cut(s) 237, 248, 1391, 1436
Fsp4HI GCNGC 5 cut(s) 459, 580, 981, 1095, 1108
FspBI CTAG 2 cut(s) 155, 348
FspI TGCGCA 1 cut(s) 1141
GlaI GCGC 1 cut(s) 1141
GluI GCNGC 5 cut(s) 459, 580, 981, 1095, 1108
HaeIII GGCC 3 cut(s) 19, 438, 1233
HapII CCGG 4 cut(s) 420, 434, 988, 1272
HgaI GACGC 1 cut(s) 274
HhaI GCGC 1 cut(s) 1142
Hin6I GCGC 1 cut(s) 1140
HinP1I GCGC 1 cut(s) 1140
HinfI GANTC 4 cut(s) 184, 329, 1225, 1301
HpaII CCGG 4 cut(s) 420, 434, 988, 1272
HphI GGTGA 5 cut(s) 62, 136, 187, 1087, 1457
Hpy166II GTNNAC 3 cut(s) 53, 68, 1181
Hpy188I TCNGA 8 cut(s) 217, 334, 448, 544, 994, 1168, 1262, 1378
Hpy188III TCNNGA 6 cut(s) 32, 104, 155, 172, 1013, 1342
Hpy8I GTNNAC 3 cut(s) 53, 68, 1181
HpyAV CCTTC 6 cut(s) 115, 798, 915, 1035, 1052, 1463
HpyCH4III ACNGT 9 cut(s) 65, 115, 613, 735, 777, 901, 1204, 1369, 1433
HpyCH4IV ACGT 6 cut(s) 483, 654, 966, 1186, 1218, 1257
HpyF10VI GCNNNNNNNGC 6 cut(s) 353, 464, 521, 530, 635, 1113
HpyF3I CTNAG 5 cut(s) 445, 605, 909, 1393, 1399
HpySE526I ACGT 6 cut(s) 483, 654, 966, 1186, 1218, 1257
HspAI GCGC 1 cut(s) 1140
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
LmnI GCTCC 2 cut(s) 504, 998
LpnPI CCDG 7 cut(s) 45, 61, 433, 447, 678, 1001, 1285
Lsp1109I GCAGC 2 cut(s) 445, 1081
LweI GCATC 2 cut(s) 748, 994
MaeI CTAG 2 cut(s) 155, 348
MaeII ACGT 6 cut(s) 483, 654, 966, 1186, 1218, 1257
MaeIII GTNAC 4 cut(s) 203, 659, 895, 1445
MalI GATC 8 cut(s) 30, 390, 617, 633, 831, 915, 1375, 1459
MbiI CCGCTC 1 cut(s) 983
MboI GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
MboII GAAGA 9 cut(s) 143, 204, 433, 783, 856, 887, 930, 1321, 1361
MfeI CAATTG 1 cut(s) 380
MflI RGATCY 1 cut(s) 631
MluCI AATT 4 cut(s) 364, 380, 868, 939
MlyI GAGTC 2 cut(s) 1219, 1310
MmeI TCCRAC 3 cut(s) 666, 1110, 1351
MseI TTAA 4 cut(s) 249, 300, 1080, 1199
MslI CAYNNNNRTG 2 cut(s) 116, 1035
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 4 cut(s) 420, 434, 988, 1272
MspR9I CCNGG 2 cut(s) 421, 435
MunI CAATTG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 99
MvnI CGCG 2 cut(s) 467, 1110
MwoI GCNNNNNNNGC 6 cut(s) 353, 464, 521, 530, 635, 1113
NciI CCSGG 2 cut(s) 421, 435
NdeII GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
NlaIV GGNNCC 3 cut(s) 375, 506, 633
NmuCI GTSAC 3 cut(s) 659, 895, 1445
NsbI TGCGCA 1 cut(s) 1141
NspI RCATGY 1 cut(s) 308
OliI CACNNNNGTG 1 cut(s) 116
PcsI WCGNNNNNNNCGW 2 cut(s) 1183, 1382
PctI GAATGC 1 cut(s) 99
PfeI GAWTC 2 cut(s) 184, 329
PkrI GCNGC 5 cut(s) 460, 581, 982, 1096, 1109
PleI GAGTC 2 cut(s) 1219, 1309
PmaCI CACGTG 1 cut(s) 655
PmlI CACGTG 1 cut(s) 655
PpsI GAGTC 2 cut(s) 1219, 1309
Ppu21I YACGTR 2 cut(s) 655, 1187
PsiI TTATAA 1 cut(s) 341
PspCI CACGTG 1 cut(s) 655
PspN4I GGNNCC 3 cut(s) 375, 506, 633
PspPI GGNCC 3 cut(s) 65, 436, 598
PsuI RGATCY 1 cut(s) 631
RsaI GTAC 5 cut(s) 316, 610, 824, 946, 1182
RsaNI GTAC 5 cut(s) 315, 609, 823, 945, 1181
RseI CAYNNNNRTG 2 cut(s) 116, 1035
SaqAI TTAA 4 cut(s) 249, 300, 1080, 1199
SatI GCNGC 5 cut(s) 459, 580, 981, 1095, 1108
Sau3AI GATC 8 cut(s) 28, 388, 615, 631, 829, 913, 1373, 1457
Sau96I GGNCC 3 cut(s) 65, 436, 598
ScaI AGTACT 2 cut(s) 610, 824
SchI GAGTC 2 cut(s) 1219, 1310
ScrFI CCNGG 2 cut(s) 421, 435
SfaNI GCATC 2 cut(s) 748, 994
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 2 cut(s) 65, 598
SmiMI CAYNNNNRTG 2 cut(s) 116, 1035
SmlI CTYRAG 2 cut(s) 1227, 1334
SmoI CTYRAG 2 cut(s) 1227, 1334
Sse9I AATT 4 cut(s) 364, 380, 868, 939
SsiI CCGC 8 cut(s) 467, 580, 629, 636, 981, 1108, 1409, 1427
SspMI CTAG 2 cut(s) 155, 348
StyD4I CCNGG 2 cut(s) 419, 433
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 9 cut(s) 65, 115, 613, 735, 777, 901, 1204, 1369, 1433
TaiI ACGT 6 cut(s) 486, 657, 969, 1189, 1221, 1260
TaqI TCGA 5 cut(s) 39, 860, 916, 1284, 1456
TaqII GACCGA 1 cut(s) 444
TasI AATT 4 cut(s) 364, 380, 868, 939
TatI WGTACW 4 cut(s) 608, 822, 944, 1180
TauI GCSGC 3 cut(s) 582, 983, 1110
TfiI GAWTC 2 cut(s) 184, 329
Tru1I TTAA 4 cut(s) 249, 300, 1080, 1199
Tru9I TTAA 4 cut(s) 249, 300, 1080, 1199
TscAI CASTG 3 cut(s) 280, 906, 1311
TseFI GTSAC 3 cut(s) 659, 895, 1445
TseI GCWGC 2 cut(s) 458, 1094
Tsp45I GTSAC 3 cut(s) 659, 895, 1445
TspDTI ATGAA 6 cut(s) 197, 220, 728, 1012, 1362, 1395
TspGWI ACGGA 4 cut(s) 191, 608, 1070, 1199
TspRI CASTG 3 cut(s) 280, 906, 1311
VpaK11BI GGWCC 2 cut(s) 65, 598
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 308
XspI CTAG 2 cut(s) 155, 348
ZrmI AGTACT 2 cut(s) 610, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.