FvH4_2g28840

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011257
Physical Location & Seq
Reverse (-)
22502766 .. 22506260
3495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28840.t3

Sequence Viewer

Length: 1458 bp
ATGAAGAAAGTTTGTTGGCCTTACTTTGATCCTGACTTTGATAAACTCCCAGAAAGGATCTATGGCCCTGTATGTAGAGTATCCATTGACAACGACACGTTGGAAGATTGCACATTAGTAAAGGTAGACAGCGTGAACAAGCAAGGGCTTCTCCTCGAAGTGATTCAAGTATTGACAGACCTGAATCTTACCATCACAAAATGTTTTATATCTTCCGATGCAGAATGGTTCATGGATGTTTTTCATGTTAAAGATGAGAACGGCAACAAACTTGCAGACCAGAATGTCATCAACTATATCCAGCAGGCCATAGGTACAACTACAGGTCCTCCAGCCTCCACCAGGGCCAATGCCTATGCCAACAATATTTTCGAATCCGAAAACCATAGCGGGCATACCACCATCGAAATGACGGGAACTGACCGCCCTGGTCTCTTCTCCGAGATATCGGCTGCCCTAGCCGACCTTCACTGCAACATAGTTGAAGCTCATGCATGGACACATAACGCCCGCCTAGCTTGTGTTGCTCAAATATCCGACAAATCCACTGACGCCCCAATCAACGATCCTCACCGCCTCGCCACCATCGAAGACCACCTCATCACAGTCCTTCGCGCCACCACCGCCGTCAGCCCTTGCGGGAAAGAAGCAAGCAACCAAGAAGTGAAGACCACAGGGTTTCTAGGAGGAGACTATCAAGGCACCATGACTGACGTCGAAAGGAGGTTGCACCAACTAATGCTTTCCATTAGGGACTTTGATGGCCCTAGTTTGAGGCCGGCAAGCTCTCCGAGGACGCCGTTGGGATGGGACGCTGAGGAAGACTGTAGGAAGACGGCTGTTTGGATCGAGAGTTGTGAGGAGAAAGGGTATTCCATGGTGAGCTTAGAGTGTAAGGATAGGCCGAGGCTTATGTTTGATACTGTGTGCACTCTAACTGACATGCAGTATGTGATTTTCCACGCTTCCACCCATTGCCACGAAGGATATGCCTTTCAGGAATATTTCATCAGACATGTTGATGGGGAGGCCTTGTGTACAGAGAGCGAGAAGGAACGAGTTATAAAATGCCTAGAGGCTGCTATTGAGCGTCGAGTTTGTGAGGGGATTAGGCTAGAGTTGCACGCCGATGATAGGATGGGATTGCTATCGGATATAACTAGGGTTCTACGAGAGAATGGACTTGCTGTTGTTCGAGCAGATGTAGCAACTCAAGGAGAGAAGGCGATCAATGCTTTCTACGTGAGGGACATTTCAGGGAACGAAGTAGTTCCGAACATGGAGTTCGTTGAGTCCATGAAGAGAGAATTGATGGGTCCAAGTCATATAAACCTTCAAGTCAAAAATGGGAAACATCTCATCAGATCACCAAGCTCAACTGAAAAGTCTCGTTATTTCGCAAATATTGGAGATATTCTAAAATCTCAAATTGGGCGTTTCTCTTTTAGAATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

486

Amino Acids

54.43

Weight (kDa)

5.39

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT_ACR9_3rd PF24931 27 - 101 9.4e-06 ACR9-like, ACT-like domain
ACT PF01842 133 - 168 2.8e-06 ACT domain
ACT_6 PF13740 135 - 179 4.4e-06 ACT domain
ACT PF01842 371 - 421 2.2e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1064
AasI GACNNNNNNGTC 1 cut(s) 284
AatII GACGTC 1 cut(s) 717
AccB1I GGYRCC 1 cut(s) 701
AccI GTMKAC 1 cut(s) 126
AccII CGCG 1 cut(s) 615
AciI CCGC 6 cut(s) 390, 424, 511, 574, 624, 639
AclWI GGATC 4 cut(s) 23, 65, 560, 854
AcyI GRCGYC 3 cut(s) 552, 714, 797
AfaI GTAC 2 cut(s) 316, 1039
AfiI CCNNNNNNNGG 2 cut(s) 342, 1134
AflIII ACRYGT 2 cut(s) 96, 1015
AgsI TTSAA 3 cut(s) 167, 485, 1337
AjnI CCWGG 2 cut(s) 341, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AluBI AGCT 5 cut(s) 488, 518, 786, 885, 1374
AluI AGCT 5 cut(s) 488, 518, 786, 885, 1374
Alw21I GWGCWC 1 cut(s) 932
Alw26I GTCTC 3 cut(s) 437, 684, 1392
Alw44I GTGCAC 1 cut(s) 928
AlwI GGATC 4 cut(s) 23, 65, 560, 854
AoxI GGCC 8 cut(s) 17, 64, 306, 345, 763, 776, 902, 1029
ApaLI GTGCAC 1 cut(s) 928
ApeKI GCWGC 2 cut(s) 452, 1079
Asp700I GAANNNNTTC 2 cut(s) 162, 1269
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 5 cut(s) 65, 326, 345, 764, 1316
AsuHPI GGTGA 3 cut(s) 563, 892, 1359
AsuII TTCGAA 1 cut(s) 372
AvaII GGWCC 2 cut(s) 326, 1316
BaeGI GKGCMC 1 cut(s) 932
BanI GGYRCC 1 cut(s) 701
BbsI GAAGAC 4 cut(s) 597, 674, 828, 839
Bbv12I GWGCWC 1 cut(s) 932
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 2 cut(s) 439, 1066
BccI CCATC 8 cut(s) 200, 410, 593, 755, 801, 1016, 1132, 1306
BceAI ACGGC 4 cut(s) 277, 611, 784, 852
BcgI CGANNNNNNTGC 2 cut(s) 971, 1005
BciT130I CCWGG 2 cut(s) 343, 429
BciVI GTATCC 1 cut(s) 91
BcoDI GTCTC 3 cut(s) 437, 684, 1392
BfaI CTAG 7 cut(s) 458, 515, 683, 768, 1073, 1115, 1161
BfmI CTRYAG 2 cut(s) 321, 826
BfuI GTATCC 1 cut(s) 91
BisI GCNGC 2 cut(s) 453, 1080
BlsI GCNGC 2 cut(s) 454, 1081
Bme1390I CCNGG 2 cut(s) 343, 429
Bme18I GGWCC 2 cut(s) 326, 1316
BmgT120I GGNCC 5 cut(s) 65, 326, 345, 764, 1316
BmiI GGNNCC 2 cut(s) 703, 1317
BmrFI CCNGG 2 cut(s) 343, 429
BmsI GCATC 1 cut(s) 208
BoxI GACNNNNGTC 1 cut(s) 713
BpiI GAAGAC 4 cut(s) 597, 674, 828, 839
BpmI CTGGAG 1 cut(s) 315
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 1197
BsaAI YACGTR 1 cut(s) 1243
BsaBI GATNNNNATC 1 cut(s) 1147
BsaHI GRCGYC 3 cut(s) 552, 714, 797
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 5 cut(s) 342, 427, 791, 876, 905
BsaXI ACNNNNNCTCC 6 cut(s) 313, 343, 854, 884, 1019, 1049
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 1134
Bse118I RCCGGY 1 cut(s) 778
Bse3DI GCAATG 1 cut(s) 973
Bse8I GATNNNNATC 1 cut(s) 1147
BseBI CCWGG 2 cut(s) 343, 429
BseDI CCNNGG 5 cut(s) 342, 427, 791, 876, 905
BseGI GGATG 3 cut(s) 241, 812, 1143
BseJI GATNNNNATC 1 cut(s) 1147
BseLI CCNNNNNNNGG 2 cut(s) 342, 1134
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 1 cut(s) 807
BseRI GAGGAG 3 cut(s) 143, 702, 875
BseSI GKGCMC 1 cut(s) 932
BseXI GCAGC 2 cut(s) 439, 1066
Bsh1236I CGCG 1 cut(s) 615
BshFI GGCC 8 cut(s) 19, 66, 308, 347, 765, 778, 904, 1031
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 932
BsiSI CCGG 1 cut(s) 779
BslFI GGGAC 3 cut(s) 767, 824, 1262
BslI CCNNNNNNNGG 2 cut(s) 342, 1134
BsmAI GTCTC 3 cut(s) 437, 684, 1392
BsmFI GGGAC 3 cut(s) 767, 824, 1262
BsnI GGCC 8 cut(s) 19, 66, 308, 347, 765, 778, 904, 1031
Bso31I GGTCTC 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 932
Bsp1407I TGTACA 1 cut(s) 1037
Bsp143I GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
Bsp19I CCATGG 1 cut(s) 876
BspACI CCGC 6 cut(s) 390, 424, 511, 574, 624, 639
BspANI GGCC 8 cut(s) 19, 66, 308, 347, 765, 778, 904, 1031
BspCNI CTCAG 1 cut(s) 808
BspFNI CGCG 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 703, 1317
BspPI GGATC 4 cut(s) 23, 65, 560, 854
BspT104I TTCGAA 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 701
BspTNI GGTCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 973
BsrFI RCCGGY 1 cut(s) 778
BsrGI TGTACA 1 cut(s) 1037
BssAI RCCGGY 1 cut(s) 778
BssECI CCNNGG 5 cut(s) 342, 427, 791, 876, 905
BssMI GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
BssNI GRCGYC 3 cut(s) 552, 714, 797
BssT1I CCWWGG 1 cut(s) 876
Bst2UI CCWGG 2 cut(s) 343, 429
Bst4CI ACNGT 3 cut(s) 607, 827, 925
Bst6I CTCTTC 2 cut(s) 440, 1295
BstACI GRCGYC 3 cut(s) 552, 714, 797
BstAUI TGTACA 1 cut(s) 1037
BstBAI YACGTR 1 cut(s) 1243
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 7 cut(s) 306, 392, 511, 652, 780, 784, 1125
BstDEI CTNAG 2 cut(s) 816, 886
BstDSI CCRYGG 1 cut(s) 876
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 3 cut(s) 241, 812, 1143
BstFNI CGCG 1 cut(s) 615
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 6 cut(s) 31, 60, 568, 849, 1230, 1367
BstMAI GTCTC 3 cut(s) 437, 684, 1392
BstMBI GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
BstMWI GCNNNNNNNGC 7 cut(s) 458, 515, 524, 623, 1120, 1205, 1232
BstNI CCWGG 2 cut(s) 343, 429
BstNSI RCATGY 2 cut(s) 946, 1019
BstPAI GACNNNNGTC 1 cut(s) 713
BstSCI CCNGG 2 cut(s) 341, 427
BstSFI CTRYAG 2 cut(s) 321, 826
BstSLI GKGCMC 1 cut(s) 932
BstUI CGCG 1 cut(s) 615
BstV1I GCAGC 2 cut(s) 439, 1066
BstV2I GAAGAC 4 cut(s) 597, 674, 828, 839
BstX2I RGATCY 1 cut(s) 57
BstYI RGATCY 1 cut(s) 57
BsuI GTATCC 1 cut(s) 91
BsuRI GGCC 8 cut(s) 19, 66, 308, 347, 765, 778, 904, 1031
BtgI CCRYGG 1 cut(s) 876
BtsCI GGATG 3 cut(s) 241, 812, 1143
BtsI GCAGTG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 469, 546
Cac8I GCNNGC 7 cut(s) 306, 392, 511, 652, 780, 784, 1125
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 1 cut(s) 778
Cfr13I GGNCC 5 cut(s) 65, 326, 345, 764, 1316
CseI GACGC 4 cut(s) 560, 805, 821, 1079
Csp6I GTAC 2 cut(s) 315, 1038
CviQI GTAC 2 cut(s) 315, 1038
DdeI CTNAG 2 cut(s) 816, 886
DpnI GATC 6 cut(s) 30, 59, 567, 848, 1229, 1366
DpnII GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
Eam1104I CTCTTC 2 cut(s) 440, 1295
EarI CTCTTC 2 cut(s) 440, 1295
Eco130I CCWWGG 1 cut(s) 876
Eco147I AGGCCT 1 cut(s) 1031
Eco31I GGTCTC 1 cut(s) 437
Eco32I GATATC 1 cut(s) 447
Eco47I GGWCC 2 cut(s) 326, 1316
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 341, 427
EcoRV GATATC 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 876
EcoT22I ATGCAT 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 876
FaqI GGGAC 3 cut(s) 767, 824, 1262
FauI CCCGC 3 cut(s) 383, 518, 632
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 2 cut(s) 453, 1080
FokI GGATG 3 cut(s) 248, 819, 1150
Fsp4HI GCNGC 2 cut(s) 453, 1080
FspBI CTAG 7 cut(s) 458, 515, 683, 768, 1073, 1115, 1161
GlaI GCGC 1 cut(s) 616
GluI GCNGC 2 cut(s) 453, 1080
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 8 cut(s) 19, 66, 308, 347, 765, 778, 904, 1031
HapII CCGG 1 cut(s) 779
HgaI GACGC 4 cut(s) 560, 805, 821, 1079
HhaI GCGC 1 cut(s) 617
Hin1I GRCGYC 3 cut(s) 552, 714, 797
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HinfI GANTC 5 cut(s) 163, 184, 374, 1292, 1449
HpaII CCGG 1 cut(s) 779
HphI GGTGA 3 cut(s) 563, 892, 1359
Hpy166II GTNNAC 4 cut(s) 127, 136, 930, 1038
Hpy188I TCNGA 9 cut(s) 217, 379, 442, 538, 792, 1013, 1153, 1275, 1364
Hpy188III TCNNGA 3 cut(s) 32, 850, 998
Hpy8I GTNNAC 4 cut(s) 127, 136, 930, 1038
Hpy99I CGWCG 2 cut(s) 719, 1095
HpyAV CCTTC 6 cut(s) 476, 620, 977, 1045, 1216, 1343
HpyCH4III ACNGT 3 cut(s) 607, 827, 925
HpyCH4IV ACGT 3 cut(s) 98, 714, 1242
HpyCH4V TGCA 9 cut(s) 111, 221, 275, 474, 494, 730, 930, 946, 1123
HpyF10VI GCNNNNNNNGC 7 cut(s) 458, 515, 524, 623, 1120, 1205, 1232
HpyF3I CTNAG 2 cut(s) 816, 886
HpySE526I ACGT 3 cut(s) 98, 714, 1242
Hsp92I GRCGYC 3 cut(s) 552, 714, 797
HspAI GCGC 1 cut(s) 615
KroI GCCGGC 1 cut(s) 778
KroNI GCCGGC 1 cut(s) 780
Kzo9I GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
Lsp1109I GCAGC 2 cut(s) 439, 1066
LweI GCATC 1 cut(s) 208
MaeI CTAG 7 cut(s) 458, 515, 683, 768, 1073, 1115, 1161
MaeII ACGT 3 cut(s) 98, 714, 1242
MalI GATC 6 cut(s) 30, 59, 567, 848, 1229, 1366
MboI GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
MboII GAAGA 9 cut(s) 16, 116, 204, 427, 602, 679, 833, 844, 1312
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 932
MluCI AATT 2 cut(s) 1307, 1428
MlyI GAGTC 1 cut(s) 1301
MmeI TCCRAC 2 cut(s) 81, 561
Mph1103I ATGCAT 1 cut(s) 496
MroNI GCCGGC 1 cut(s) 778
MroXI GAANNNNTTC 2 cut(s) 162, 1269
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 3 cut(s) 407, 1020, 1128
MspI CCGG 1 cut(s) 779
MspR9I CCNGG 2 cut(s) 343, 429
MvaI CCWGG 2 cut(s) 343, 429
MvnI CGCG 1 cut(s) 615
MwoI GCNNNNNNNGC 7 cut(s) 458, 515, 524, 623, 1120, 1205, 1232
NaeI GCCGGC 1 cut(s) 780
NcoI CCATGG 1 cut(s) 876
NdeII GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
NgoMIV GCCGGC 1 cut(s) 778
NlaIV GGNNCC 2 cut(s) 703, 1317
NmeAIII GCCGAG 1 cut(s) 930
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 2 cut(s) 946, 1019
NspV TTCGAA 1 cut(s) 372
PceI AGGCCT 1 cut(s) 1031
PciI ACATGT 1 cut(s) 1015
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PdiI GCCGGC 1 cut(s) 780
PdmI GAANNNNTTC 2 cut(s) 162, 1269
PfeI GAWTC 4 cut(s) 163, 184, 374, 1449
PkrI GCNGC 2 cut(s) 454, 1081
PleI GAGTC 1 cut(s) 1300
PpsI GAGTC 1 cut(s) 1300
Ppu21I YACGTR 1 cut(s) 1243
PpuMI RGGWCCY 1 cut(s) 326
PscI ACATGT 1 cut(s) 1015
PshAI GACNNNNGTC 1 cut(s) 713
PsiI TTATAA 1 cut(s) 1064
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 341, 427
PspGI CCWGG 2 cut(s) 341, 427
PspN4I GGNNCC 2 cut(s) 703, 1317
PspPI GGNCC 5 cut(s) 65, 326, 345, 764, 1316
PspPPI RGGWCCY 1 cut(s) 326
PsuI RGATCY 1 cut(s) 57
RsaI GTAC 2 cut(s) 316, 1039
RsaNI GTAC 2 cut(s) 315, 1038
RseI CAYNNNNRTG 3 cut(s) 407, 1020, 1128
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 2 cut(s) 453, 1080
Sau3AI GATC 6 cut(s) 28, 57, 565, 846, 1227, 1364
Sau96I GGNCC 5 cut(s) 65, 326, 345, 764, 1316
SchI GAGTC 1 cut(s) 1301
ScrFI CCNGG 2 cut(s) 343, 429
SduI GDGCHC 1 cut(s) 932
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 2 cut(s) 321, 826
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 2 cut(s) 326, 1316
SmiMI CAYNNNNRTG 3 cut(s) 407, 1020, 1128
SmlI CTYRAG 1 cut(s) 1212
SmoI CTYRAG 1 cut(s) 1212
Sse9I AATT 2 cut(s) 1307, 1428
SseBI AGGCCT 1 cut(s) 1031
SsiI CCGC 6 cut(s) 390, 424, 511, 574, 624, 639
SspI AATATT 3 cut(s) 367, 1004, 1405
SspMI CTAG 7 cut(s) 458, 515, 683, 768, 1073, 1115, 1161
StuI AGGCCT 1 cut(s) 1031
StyD4I CCNGG 2 cut(s) 341, 427
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 3 cut(s) 607, 827, 925
TaiI ACGT 3 cut(s) 101, 717, 1245
TaqI TCGA 8 cut(s) 156, 372, 405, 588, 717, 849, 1093, 1195
TasI AATT 2 cut(s) 1307, 1428
TatI WGTACW 1 cut(s) 1037
TfiI GAWTC 4 cut(s) 163, 184, 374, 1449
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 2 cut(s) 476, 553
TseI GCWGC 2 cut(s) 452, 1079
TspDTI ATGAA 5 cut(s) 17, 220, 233, 997, 1313
TspRI CASTG 2 cut(s) 476, 553
VneI GTGCAC 1 cut(s) 928
VpaK11BI GGWCC 2 cut(s) 326, 1316
XagI CCTNNNNNAGG 1 cut(s) 340
XceI RCATGY 2 cut(s) 946, 1019
XmiI GTMKAC 1 cut(s) 126
XmnI GAANNNNTTC 2 cut(s) 162, 1269
XspI CTAG 7 cut(s) 458, 515, 683, 768, 1073, 1115, 1161
ZraI GACGTC 1 cut(s) 715
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.