RLG00000011578

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011257
Physical Location & Seq
Forward (+)
10671920 .. 10674228
2309 bp
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UTR
Exon/CDS
Intron
RLM00000011578

Sequence Viewer

Length: 1434 bp
ATGAAGAAAGTTTGTTGGCCTTACTTTGATCCTGACTTTCATAATCTCCCAGAAAGGATCTATGGCCCTGTATGTAGAGTATCCCTCGACAACGAGACGTTGGAAGATTGCACATTAGTAAAGGTTTGTCAGTACTTCTGTGGATCAAGAACAAACATAATTTCAGGCAAGTATATGACTTATGCTTATGATATTAAATTATTAATTGTATGGGTTTTTCATGTTAAAGATGAGAATGGCAACAAACTTACAGACCAGAATGCCATTAACTATATCCAGCAGGCCATAGGTACAACTACAGGTCCTCCAGACTCCACCAGGGCCAATGCCTATGCCAGCAATATTTTCGAATCCGAAAACCATAGCGAGCATACGACCATCGAAATGACGGGAACGGACCGCCCTGGTCTCTTCTCGGAGATATCGGCTGCCCTTGCCGACCTCCAATGCAACATAGTTGAAGCCCATGCGTGGACCCATAACGGCCGGCTAGCTTGTGTTGCTCAAATATCTGACCAATCCACTGACGCCCCAATCGAGGACCCCCACCGCCTCGCCACCATCGAAGACCACCTCATCACCGTTCTTCGAGCCACCACTTCCGTAAGCCCTTGTGGGAAAGAAGCAAACAACCAAGAAGTGAAGACCACAGGGTTTTTAGGAGGAGACTATCAAGGCACCATGACTAATGTCGAAAGGAGGTTACACCAACTAATGCTTTCCATTAGGGACTTTGACTGCCCCAATTTGAGGCCCGCCAGCTCTCCGAGGACGCCATTTGCATGGGATGCTGAGGAAGAGTGTAGGAAGACGGCGGTTTGGATCGAGAGCTGCAAGGAGAAAGGGTATTCCATGGTGAGCTTAGAGTGTAAGGATCGGCCGAGGCTTATGTTTGATACTGTGTGCACTCTAACTGACATGCAGTATGTGATTTTCCACGCTTCCACTCATTGCCATGAAGGATATGCCTTTCAGGAATACTTTATCCGGCATGTTGACGGGGATGTCTTGTGCACAGAGAGCGAGAAGGAACGAGTTATAAAATGCTTAGAGGCTGCTATTGAGCGTCGAGTTTGTGAGGGGATTCGACTAGAGTTGCGTGCCGACGATAGGATAGGATTGCTATCGGATATAACTAGGGTTCTACGAGAGAATGGACTTGCTGTTGTTCGAGCAGACGTTGCGACTCAAGGAGAGAAGTCGATTAACGCTTTCTATGTGAGGGACATTTCAGGGAACGAAGTAGTTCCAGACATGGATTTTGTTGAGTCCATGAAGAGAGAATTGATGGGTCCAAGTCATATAAACCTTCAAGTCAAGAATGAGAAACATCTCAGATCACCAAGCTCAACTGAAAAGTCTCGTTATTTCTCAAATATTGGAGATATTCTAAAATCTCAATTTGGGCGTTTCTCTTTTGCTAAGATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

478

Amino Acids

54.03

Weight (kDa)

5.59

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 125 - 162 1.8e-06 ACT domain
ACT_6 PF13740 127 - 171 3.2e-07 ACT domain
ACT PF01842 363 - 413 6.4e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1040
AasI GACNNNNNNGTC 1 cut(s) 1004
AccB1I GGYRCC 1 cut(s) 677
AciI CCGC 4 cut(s) 400, 550, 756, 815
AclWI GGATC 5 cut(s) 23, 65, 151, 830, 882
AcoI YGGCCR 2 cut(s) 484, 878
AcyI GRCGYC 2 cut(s) 528, 773
AfaI GTAC 2 cut(s) 134, 292
AfiI CCNNNNNNNGG 4 cut(s) 471, 538, 750, 1110
AgsI TTSAA 2 cut(s) 461, 1313
AjnI CCWGG 2 cut(s) 317, 403
AluBI AGCT 5 cut(s) 494, 762, 831, 861, 1347
AluI AGCT 5 cut(s) 494, 762, 831, 861, 1347
Alw21I GWGCWC 2 cut(s) 908, 1016
Alw26I GTCTC 4 cut(s) 89, 413, 660, 1365
Alw44I GTGCAC 2 cut(s) 904, 1012
AlwI GGATC 5 cut(s) 23, 65, 151, 830, 882
AoxI GGCC 7 cut(s) 17, 64, 282, 321, 484, 752, 878
ApaLI GTGCAC 2 cut(s) 904, 1012
ApeKI GCWGC 3 cut(s) 428, 831, 1055
ArsI GACNNNNNNTTYG 2 cut(s) 1244, 1276
AseI ATTAAT 1 cut(s) 203
Asp700I GAANNNNTTC 1 cut(s) 1245
AspS9I GGNCC 8 cut(s) 65, 302, 321, 397, 474, 541, 753, 1292
AsuHPI GGTGA 3 cut(s) 571, 868, 1332
AsuII TTCGAA 1 cut(s) 348
AsuNHI GCTAGC 1 cut(s) 490
AvaII GGWCC 5 cut(s) 302, 397, 474, 541, 1292
BaeGI GKGCMC 2 cut(s) 908, 1016
BanI GGYRCC 1 cut(s) 677
BbsI GAAGAC 3 cut(s) 573, 650, 815
Bbv12I GWGCWC 2 cut(s) 908, 1016
BbvCI CCTCAGC 1 cut(s) 792
BbvI GCAGC 3 cut(s) 415, 818, 1042
BccI CCATC 3 cut(s) 386, 569, 1282
BceAI ACGGC 2 cut(s) 499, 828
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 2 cut(s) 319, 405
BciVI GTATCC 1 cut(s) 91
BcoDI GTCTC 4 cut(s) 89, 413, 660, 1365
BfaI CTAG 3 cut(s) 491, 1091, 1137
BfmI CTRYAG 1 cut(s) 297
BfuI GTATCC 1 cut(s) 91
BisI GCNGC 3 cut(s) 429, 832, 1056
BlsI GCNGC 3 cut(s) 430, 833, 1057
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 2 cut(s) 319, 405
Bme18I GGWCC 5 cut(s) 302, 397, 474, 541, 1292
BmgT120I GGNCC 8 cut(s) 65, 302, 321, 397, 474, 541, 753, 1292
BmiI GGNNCC 4 cut(s) 476, 543, 679, 1293
BmrFI CCNGG 2 cut(s) 319, 405
BmsI GCATC 1 cut(s) 778
BmtI GCTAGC 1 cut(s) 494
BoxI GACNNNNGTC 1 cut(s) 689
BpiI GAAGAC 3 cut(s) 573, 650, 815
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 1173
BsaBI GATNNNNATC 1 cut(s) 1123
BsaHI GRCGYC 2 cut(s) 528, 773
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 5 cut(s) 318, 403, 767, 852, 881
BsaXI ACNNNNNCTCC 4 cut(s) 289, 319, 830, 860
Bsc4I CCNNNNNNNGG 4 cut(s) 471, 538, 750, 1110
Bse118I RCCGGY 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 949
Bse8I GATNNNNATC 1 cut(s) 1123
BseBI CCWGG 2 cut(s) 319, 405
BseDI CCNNGG 5 cut(s) 318, 403, 767, 852, 881
BseGI GGATG 2 cut(s) 793, 1009
BseJI GATNNNNATC 1 cut(s) 1123
BseLI CCNNNNNNNGG 4 cut(s) 471, 538, 750, 1110
BseMI GCAATG 1 cut(s) 949
BseMII CTCAG 2 cut(s) 783, 1348
BseRI GAGGAG 1 cut(s) 678
BseSI GKGCMC 2 cut(s) 908, 1016
BseX3I CGGCCG 2 cut(s) 484, 878
BseXI GCAGC 3 cut(s) 415, 818, 1042
Bsh1285I CGRYCG 2 cut(s) 487, 881
BshFI GGCC 7 cut(s) 19, 66, 284, 323, 486, 754, 880
BshNI GGYRCC 1 cut(s) 677
BsiEI CGRYCG 2 cut(s) 487, 881
BsiHKAI GWGCWC 2 cut(s) 908, 1016
BsiSI CCGG 2 cut(s) 487, 988
BslFI GGGAC 2 cut(s) 743, 1238
BslI CCNNNNNNNGG 4 cut(s) 471, 538, 750, 1110
BsmAI GTCTC 4 cut(s) 89, 413, 660, 1365
BsmBI CGTCTC 1 cut(s) 89
BsmFI GGGAC 2 cut(s) 743, 1238
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 7 cut(s) 19, 66, 284, 323, 486, 754, 880
Bso31I GGTCTC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 908, 1016
Bsp143I GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
Bsp19I CCATGG 1 cut(s) 852
BspACI CCGC 4 cut(s) 400, 550, 756, 815
BspANI GGCC 7 cut(s) 19, 66, 284, 323, 486, 754, 880
BspCNI CTCAG 2 cut(s) 784, 1347
BspLI GGNNCC 4 cut(s) 476, 543, 679, 1293
BspOI GCTAGC 1 cut(s) 494
BspPI GGATC 5 cut(s) 23, 65, 151, 830, 882
BspT104I TTCGAA 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 677
BspTNI GGTCTC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 949
BsrFI RCCGGY 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 486
BssECI CCNNGG 5 cut(s) 318, 403, 767, 852, 881
BssMI GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
BssNI GRCGYC 2 cut(s) 528, 773
BssT1I CCWWGG 1 cut(s) 852
Bst2UI CCWGG 2 cut(s) 319, 405
Bst4CI ACNGT 2 cut(s) 583, 901
Bst6I CTCTTC 3 cut(s) 416, 792, 1271
BstACI GRCGYC 2 cut(s) 528, 773
BstAPI GCANNNNNTGC 2 cut(s) 788, 1181
BstBI TTCGAA 1 cut(s) 348
BstC8I GCNNGC 8 cut(s) 282, 337, 368, 488, 492, 756, 760, 1101
BstDEI CTNAG 5 cut(s) 792, 862, 1048, 1334, 1422
BstDSI CCRYGG 1 cut(s) 852
BstF5I GGATG 2 cut(s) 793, 1009
BstKTI GATC 7 cut(s) 31, 60, 146, 825, 877, 1340, 1428
BstMAI GTCTC 4 cut(s) 89, 413, 660, 1365
BstMBI GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
BstMCI CGRYCG 2 cut(s) 487, 881
BstMWI GCNNNNNNNGC 5 cut(s) 434, 500, 788, 1020, 1181
BstNI CCWGG 2 cut(s) 319, 405
BstNSI RCATGY 2 cut(s) 922, 995
BstPAI GACNNNNGTC 1 cut(s) 689
BstSCI CCNGG 2 cut(s) 317, 403
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 2 cut(s) 908, 1016
BstV1I GCAGC 3 cut(s) 415, 818, 1042
BstV2I GAAGAC 3 cut(s) 573, 650, 815
BstX2I RGATCY 1 cut(s) 57
BstXI CCANNNNNNTGG 1 cut(s) 783
BstYI RGATCY 1 cut(s) 57
BstZI CGGCCG 2 cut(s) 484, 878
BsuI GTATCC 1 cut(s) 91
BsuRI GGCC 7 cut(s) 19, 66, 284, 323, 486, 754, 880
BtgI CCRYGG 1 cut(s) 852
BtsCI GGATG 2 cut(s) 793, 1009
BtsIMutI CAGTG 1 cut(s) 522
Cac8I GCNNGC 8 cut(s) 282, 337, 368, 488, 492, 756, 760, 1101
Cfr10I RCCGGY 1 cut(s) 486
Cfr13I GGNCC 8 cut(s) 65, 302, 321, 397, 474, 541, 753, 1292
CpoI CGGWCCG 1 cut(s) 397
CseI GACGC 3 cut(s) 536, 781, 1055
Csp6I GTAC 2 cut(s) 133, 291
CspI CGGWCCG 1 cut(s) 397
CviQI GTAC 2 cut(s) 133, 291
DdeI CTNAG 5 cut(s) 792, 862, 1048, 1334, 1422
DpnI GATC 7 cut(s) 30, 59, 145, 824, 876, 1339, 1427
DpnII GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
DrdI GACNNNNNNGTC 1 cut(s) 1004
DseDI GACNNNNNNGTC 1 cut(s) 1004
EaeI YGGCCR 2 cut(s) 484, 878
EagI CGGCCG 2 cut(s) 484, 878
Eam1104I CTCTTC 3 cut(s) 416, 792, 1271
EarI CTCTTC 3 cut(s) 416, 792, 1271
EclXI CGGCCG 2 cut(s) 484, 878
Eco130I CCWWGG 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 413
Eco32I GATATC 1 cut(s) 423
Eco47I GGWCC 5 cut(s) 302, 397, 474, 541, 1292
Eco52I CGGCCG 2 cut(s) 484, 878
EcoO109I RGGNCCY 2 cut(s) 302, 541
EcoRII CCWGG 2 cut(s) 317, 403
EcoRV GATATC 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 852
Esp3I CGTCTC 1 cut(s) 89
FaqI GGGAC 2 cut(s) 743, 1238
FauI CCCGC 1 cut(s) 763
Fnu4HI GCNGC 3 cut(s) 429, 832, 1056
FokI GGATG 2 cut(s) 800, 1016
Fsp4HI GCNGC 3 cut(s) 429, 832, 1056
FspBI CTAG 3 cut(s) 491, 1091, 1137
GluI GCNGC 3 cut(s) 429, 832, 1056
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 7 cut(s) 19, 66, 284, 323, 486, 754, 880
HapII CCGG 2 cut(s) 487, 988
HgaI GACGC 3 cut(s) 536, 781, 1055
Hin1I GRCGYC 2 cut(s) 528, 773
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HinfI GANTC 5 cut(s) 311, 350, 1084, 1186, 1268
HpaII CCGG 2 cut(s) 487, 988
HphI GGTGA 3 cut(s) 571, 868, 1332
Hpy166II GTNNAC 4 cut(s) 474, 906, 997, 1014
Hpy188I TCNGA 6 cut(s) 355, 418, 514, 768, 1129, 1337
Hpy188III TCNNGA 7 cut(s) 32, 147, 308, 826, 974, 1250, 1318
Hpy8I GTNNAC 4 cut(s) 474, 906, 997, 1014
Hpy99I CGWCG 2 cut(s) 1071, 1109
HpyAV CCTTC 3 cut(s) 953, 1021, 1319
HpyCH4III ACNGT 2 cut(s) 583, 901
HpyCH4IV ACGT 2 cut(s) 98, 1179
HpyCH4V TGCA 7 cut(s) 111, 450, 782, 834, 906, 922, 1014
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 500, 788, 1020, 1181
HpyF3I CTNAG 5 cut(s) 792, 862, 1048, 1334, 1422
HpySE526I ACGT 2 cut(s) 98, 1179
Hsp92I GRCGYC 2 cut(s) 528, 773
KroI GCCGGC 1 cut(s) 486
KroNI GCCGGC 1 cut(s) 488
Kzo9I GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
Lsp1109I GCAGC 3 cut(s) 415, 818, 1042
LweI GCATC 1 cut(s) 778
MaeI CTAG 3 cut(s) 491, 1091, 1137
MaeII ACGT 2 cut(s) 98, 1179
MaeIII GTNAC 1 cut(s) 702
MalI GATC 7 cut(s) 30, 59, 145, 824, 876, 1339, 1427
MboI GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
MboII GAAGA 9 cut(s) 16, 116, 403, 578, 578, 655, 809, 820, 1288
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 2 cut(s) 908, 1016
MluCI AATT 6 cut(s) 159, 197, 204, 745, 1283, 1400
MlyI GAGTC 3 cut(s) 305, 1180, 1277
MmeI TCCRAC 1 cut(s) 81
MroNI GCCGGC 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 1245
MseI TTAA 5 cut(s) 195, 203, 225, 267, 1206
MslI CAYNNNNRTG 3 cut(s) 383, 781, 954
MspI CCGG 2 cut(s) 487, 988
MspR9I CCNGG 2 cut(s) 319, 405
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 2 cut(s) 319, 405
MwoI GCNNNNNNNGC 5 cut(s) 434, 500, 788, 1020, 1181
NaeI GCCGGC 1 cut(s) 488
NcoI CCATGG 1 cut(s) 852
NdeII GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
NgoMIV GCCGGC 1 cut(s) 486
NheI GCTAGC 1 cut(s) 490
NlaIV GGNNCC 4 cut(s) 476, 543, 679, 1293
NmeAIII GCCGAG 1 cut(s) 906
NspI RCATGY 2 cut(s) 922, 995
NspV TTCGAA 1 cut(s) 348
PcsI WCGNNNNNNNCGW 2 cut(s) 534, 561
PctI GAATGC 1 cut(s) 265
PdiI GCCGGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 2 cut(s) 350, 1084
PkrI GCNGC 3 cut(s) 430, 833, 1057
PleI GAGTC 3 cut(s) 305, 1180, 1276
PpsI GAGTC 3 cut(s) 305, 1180, 1276
PpuMI RGGWCCY 2 cut(s) 302, 541
PshAI GACNNNNGTC 1 cut(s) 689
PshBI ATTAAT 1 cut(s) 203
PsiI TTATAA 1 cut(s) 1040
Psp5II RGGWCCY 2 cut(s) 302, 541
Psp6I CCWGG 2 cut(s) 317, 403
PspGI CCWGG 2 cut(s) 317, 403
PspN4I GGNNCC 4 cut(s) 476, 543, 679, 1293
PspPI GGNCC 8 cut(s) 65, 302, 321, 397, 474, 541, 753, 1292
PspPPI RGGWCCY 2 cut(s) 302, 541
PsuI RGATCY 1 cut(s) 57
RsaI GTAC 2 cut(s) 134, 292
RsaNI GTAC 2 cut(s) 133, 291
RseI CAYNNNNRTG 3 cut(s) 383, 781, 954
Rsr2I CGGWCCG 1 cut(s) 397
RsrII CGGWCCG 1 cut(s) 397
SaqAI TTAA 5 cut(s) 195, 203, 225, 267, 1206
SatI GCNGC 3 cut(s) 429, 832, 1056
Sau3AI GATC 7 cut(s) 28, 57, 143, 822, 874, 1337, 1425
Sau96I GGNCC 8 cut(s) 65, 302, 321, 397, 474, 541, 753, 1292
ScaI AGTACT 1 cut(s) 134
SchI GAGTC 3 cut(s) 305, 1180, 1277
ScrFI CCNGG 2 cut(s) 319, 405
SduI GDGCHC 2 cut(s) 908, 1016
SfaNI GCATC 1 cut(s) 778
SfcI CTRYAG 1 cut(s) 297
SfuI TTCGAA 1 cut(s) 348
SinI GGWCC 5 cut(s) 302, 397, 474, 541, 1292
SmiMI CAYNNNNRTG 3 cut(s) 383, 781, 954
SmlI CTYRAG 1 cut(s) 1188
SmoI CTYRAG 1 cut(s) 1188
Sse9I AATT 6 cut(s) 159, 197, 204, 745, 1283, 1400
SsiI CCGC 4 cut(s) 400, 550, 756, 815
SspI AATATT 2 cut(s) 343, 1378
SspMI CTAG 3 cut(s) 491, 1091, 1137
StyD4I CCNGG 2 cut(s) 317, 403
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 2 cut(s) 583, 901
TaiI ACGT 2 cut(s) 101, 1182
TasI AATT 6 cut(s) 159, 197, 204, 745, 1283, 1400
TatI WGTACW 1 cut(s) 132
TfiI GAWTC 2 cut(s) 350, 1084
Tru1I TTAA 5 cut(s) 195, 203, 225, 267, 1206
Tru9I TTAA 5 cut(s) 195, 203, 225, 267, 1206
TscAI CASTG 1 cut(s) 529
TseI GCWGC 3 cut(s) 428, 831, 1055
TspDTI ATGAA 5 cut(s) 17, 29, 209, 972, 1289
TspGWI ACGGA 2 cut(s) 410, 592
TspRI CASTG 1 cut(s) 529
VneI GTGCAC 2 cut(s) 904, 1012
VpaK11BI GGWCC 5 cut(s) 302, 397, 474, 541, 1292
VspI ATTAAT 1 cut(s) 203
XceI RCATGY 2 cut(s) 922, 995
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 3 cut(s) 491, 1091, 1137
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.