Rh6DG385500

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011257
Physical Location & Seq
Reverse (-)
57839058 .. 57845974
6917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG385500.1

Sequence Viewer

Length: 1278 bp
ATGAATCTTACCATCACTAAATGTTTTATATCTTCTGATGCAGAATGGTTCATGGATGTTTTTCATGTTAAAGATGAGCATGGCAACAAACTTACAGACCAGAATGCCATTAACTATATCCAGCAGGCCATAGGTACAACTACAGGTCCTCCAGACTCCACCAGGGCCAATGCCTATGCCAGCAATATTTTCGAATCCGAAAACCATAGCGAGCATACGACCATCGAAATGACTGGAACGGACCGCCCTGGTCTCTTCTCGGAGATATCGGCTGCCCTTGCCGACCTCCAATGCAACATAGTTGAAGCCCATGCGTGGACCCATAACGGCCGGCTAGCTTGTGTTGCTCAAATATCTGACCAATCCACTGACGCCCCAATCGACGACCCCCACCGCCTCGCCACCATCGAAGACCACCTCATCACCGTCCTTCGAGCCACCACCGCCGTAAGCCCTTGTGGGAAAGAAGCAAACAACCAAGAAGTGAAGACCACAGGGTTTTTAGGAGGAGACTATCAAGGCACCATGACTAATGTCGAAAGGAGGTTACACCAACTAATGCTTTCCATTAGGGACTTTGATGGCCCCAGTTTGAGGCCCGCCAGCTCTCCGAGGACGCCATTTGCATGGGATGCTGAGGAAGAGTGTAGGAAGACGGCGGTTTGGATCGAGAGCTGCAAGGAGAAAGGGTATTCCATGGTGAGCTTAGAGTGTAAGGATCGGCCGAGGCTTATGTTTGATACTGTGTGCACTCTAACTGACATGCAGTATGTGATTTTCCACGCTTCCACTCATTGCCATGAAGGATATGCCTTTCAGGAATACTTTATCCGGCATGTTGACGGGGATGTCTTGTGCACAGAGAGCGAGAAGGAACGAGTTATAAAATGCTTAGAGGCTGCTATTGAGCGTCGAGTTTGTGAGGGGATTCGACTAGAGTTGCGTGCCGACGATAGGATGGGATTGCTATCGGATATAACTAGGGTTCTACGAGAGAATGGACTTGCTGTTGTTCGAGCAGATGTAGCAACCCAAGGAGAGAAGTCGATTAACGCTTTCTATGTGAGGGACATTTCAGGGAACGAAGTAGTTCCAGACATGGATTTTGTTGAGTCCATGAAGAGAGAATTGATGGGTCCAAGTCATATAAACCTTCAAGTCAAGAATGAGAAACATCTCAGATCACCAAGCTCAACTGAAAAGTCTCGTTATTTCTCAAATATTGGAGATATTCTAAAATCTCAAATTGGGCGTTTCTCTTTTGCTAAGATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

425

Amino Acids

47.78

Weight (kDa)

5.43

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 73 - 109 1.7e-06 ACT domain
ACT_6 PF13740 75 - 119 3.1e-07 ACT domain
ACT PF01842 311 - 361 2.6e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
AasI GACNNNNNNGTC 1 cut(s) 848
AccB1I GGYRCC 1 cut(s) 521
AciI CCGC 5 cut(s) 244, 394, 444, 600, 659
AclWI GGATC 2 cut(s) 674, 726
AcoI YGGCCR 2 cut(s) 328, 722
AcyI GRCGYC 2 cut(s) 372, 617
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 3 cut(s) 315, 594, 954
AgsI TTSAA 2 cut(s) 305, 1157
AjnI CCWGG 2 cut(s) 161, 247
AluBI AGCT 5 cut(s) 338, 606, 675, 705, 1191
AluI AGCT 5 cut(s) 338, 606, 675, 705, 1191
Alw21I GWGCWC 2 cut(s) 752, 860
Alw26I GTCTC 3 cut(s) 257, 504, 1209
Alw44I GTGCAC 2 cut(s) 748, 856
AlwI GGATC 2 cut(s) 674, 726
AoxI GGCC 6 cut(s) 126, 165, 328, 583, 596, 722
ApaLI GTGCAC 2 cut(s) 748, 856
ApeKI GCWGC 3 cut(s) 272, 675, 899
ArsI GACNNNNNNTTYG 2 cut(s) 1088, 1120
Asp700I GAANNNNTTC 1 cut(s) 1089
AspS9I GGNCC 7 cut(s) 146, 165, 241, 318, 584, 597, 1136
AsuHPI GGTGA 3 cut(s) 415, 712, 1176
AsuII TTCGAA 1 cut(s) 192
AsuNHI GCTAGC 1 cut(s) 334
AvaII GGWCC 4 cut(s) 146, 241, 318, 1136
BaeGI GKGCMC 2 cut(s) 752, 860
BanI GGYRCC 1 cut(s) 521
BbsI GAAGAC 3 cut(s) 417, 494, 659
Bbv12I GWGCWC 2 cut(s) 752, 860
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 3 cut(s) 259, 662, 886
BccI CCATC 6 cut(s) 20, 230, 413, 575, 952, 1126
BceAI ACGGC 3 cut(s) 343, 431, 672
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 2 cut(s) 163, 249
BcoDI GTCTC 3 cut(s) 257, 504, 1209
BfaI CTAG 3 cut(s) 335, 935, 981
BfmI CTRYAG 1 cut(s) 141
BisI GCNGC 3 cut(s) 273, 676, 900
BlsI GCNGC 3 cut(s) 274, 677, 901
Bme1390I CCNGG 2 cut(s) 163, 249
Bme18I GGWCC 4 cut(s) 146, 241, 318, 1136
BmgT120I GGNCC 7 cut(s) 146, 165, 241, 318, 584, 597, 1136
BmiI GGNNCC 4 cut(s) 320, 523, 586, 1137
BmrFI CCNGG 2 cut(s) 163, 249
BmrI ACTGGG 1 cut(s) 582
BmsI GCATC 2 cut(s) 28, 622
BmtI GCTAGC 1 cut(s) 338
BmuI ACTGGG 1 cut(s) 582
BoxI GACNNNNGTC 1 cut(s) 533
BpiI GAAGAC 3 cut(s) 417, 494, 659
BpmI CTGGAG 1 cut(s) 135
Bpu10I CCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 967
BsaHI GRCGYC 2 cut(s) 372, 617
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 6 cut(s) 162, 247, 611, 696, 725, 1033
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 674, 704
Bsc4I CCNNNNNNNGG 3 cut(s) 315, 594, 954
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 2 cut(s) 238, 588
Bse3DI GCAATG 1 cut(s) 793
Bse8I GATNNNNATC 1 cut(s) 967
BseBI CCWGG 2 cut(s) 163, 249
BseDI CCNNGG 6 cut(s) 162, 247, 611, 696, 725, 1033
BseGI GGATG 4 cut(s) 61, 637, 853, 963
BseJI GATNNNNATC 1 cut(s) 967
BseLI CCNNNNNNNGG 3 cut(s) 315, 594, 954
BseMI GCAATG 1 cut(s) 793
BseMII CTCAG 2 cut(s) 627, 1192
BseNI ACTGG 2 cut(s) 238, 588
BseRI GAGGAG 1 cut(s) 522
BseSI GKGCMC 2 cut(s) 752, 860
BseX3I CGGCCG 2 cut(s) 328, 722
BseXI GCAGC 3 cut(s) 259, 662, 886
Bsh1285I CGRYCG 2 cut(s) 331, 725
BshFI GGCC 6 cut(s) 128, 167, 330, 585, 598, 724
BshNI GGYRCC 1 cut(s) 521
BsiEI CGRYCG 2 cut(s) 331, 725
BsiHKAI GWGCWC 2 cut(s) 752, 860
BsiSI CCGG 2 cut(s) 331, 832
BslFI GGGAC 2 cut(s) 587, 1082
BslI CCNNNNNNNGG 3 cut(s) 315, 594, 954
BsmAI GTCTC 3 cut(s) 257, 504, 1209
BsmFI GGGAC 2 cut(s) 587, 1082
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 6 cut(s) 128, 167, 330, 585, 598, 724
Bso31I GGTCTC 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 752, 860
Bsp143I GATC 4 cut(s) 666, 718, 1181, 1269
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 5 cut(s) 244, 394, 444, 600, 659
BspANI GGCC 6 cut(s) 128, 167, 330, 585, 598, 724
BspCNI CTCAG 2 cut(s) 628, 1191
BspLI GGNNCC 4 cut(s) 320, 523, 586, 1137
BspOI GCTAGC 1 cut(s) 338
BspPI GGATC 2 cut(s) 674, 726
BspT104I TTCGAA 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 793
BsrFI RCCGGY 1 cut(s) 330
BsrI ACTGG 2 cut(s) 238, 588
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 6 cut(s) 162, 247, 611, 696, 725, 1033
BssMI GATC 4 cut(s) 666, 718, 1181, 1269
BssNI GRCGYC 2 cut(s) 372, 617
BssT1I CCWWGG 2 cut(s) 696, 1033
Bst2UI CCWGG 2 cut(s) 163, 249
Bst4CI ACNGT 2 cut(s) 427, 745
Bst6I CTCTTC 3 cut(s) 260, 636, 1115
BstACI GRCGYC 2 cut(s) 372, 617
BstAPI GCANNNNNTGC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 192
BstC8I GCNNGC 8 cut(s) 126, 181, 212, 332, 336, 600, 604, 945
BstDEI CTNAG 5 cut(s) 636, 706, 892, 1178, 1266
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 4 cut(s) 61, 637, 853, 963
BstKTI GATC 4 cut(s) 669, 721, 1184, 1272
BstMAI GTCTC 3 cut(s) 257, 504, 1209
BstMBI GATC 4 cut(s) 666, 718, 1181, 1269
BstMCI CGRYCG 2 cut(s) 331, 725
BstMWI GCNNNNNNNGC 6 cut(s) 278, 344, 443, 632, 864, 1025
BstNI CCWGG 2 cut(s) 163, 249
BstNSI RCATGY 2 cut(s) 766, 839
BstPAI GACNNNNGTC 1 cut(s) 533
BstSCI CCNGG 2 cut(s) 161, 247
BstSFI CTRYAG 1 cut(s) 141
BstSLI GKGCMC 2 cut(s) 752, 860
BstV1I GCAGC 3 cut(s) 259, 662, 886
BstV2I GAAGAC 3 cut(s) 417, 494, 659
BstXI CCANNNNNNTGG 1 cut(s) 627
BstZI CGGCCG 2 cut(s) 328, 722
BsuRI GGCC 6 cut(s) 128, 167, 330, 585, 598, 724
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 4 cut(s) 61, 637, 853, 963
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 8 cut(s) 126, 181, 212, 332, 336, 600, 604, 945
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 7 cut(s) 146, 165, 241, 318, 584, 597, 1136
CpoI CGGWCCG 1 cut(s) 241
CseI GACGC 3 cut(s) 380, 625, 899
Csp6I GTAC 1 cut(s) 135
CspI CGGWCCG 1 cut(s) 241
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 5 cut(s) 636, 706, 892, 1178, 1266
DpnI GATC 4 cut(s) 668, 720, 1183, 1271
DpnII GATC 4 cut(s) 666, 718, 1181, 1269
DrdI GACNNNNNNGTC 1 cut(s) 848
DseDI GACNNNNNNGTC 1 cut(s) 848
EaeI YGGCCR 2 cut(s) 328, 722
EagI CGGCCG 2 cut(s) 328, 722
Eam1104I CTCTTC 3 cut(s) 260, 636, 1115
EarI CTCTTC 3 cut(s) 260, 636, 1115
EclXI CGGCCG 2 cut(s) 328, 722
Eco130I CCWWGG 2 cut(s) 696, 1033
Eco31I GGTCTC 1 cut(s) 257
Eco32I GATATC 1 cut(s) 267
Eco47I GGWCC 4 cut(s) 146, 241, 318, 1136
Eco52I CGGCCG 2 cut(s) 328, 722
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 161, 247
EcoRV GATATC 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 696, 1033
ErhI CCWWGG 2 cut(s) 696, 1033
FaqI GGGAC 2 cut(s) 587, 1082
FauI CCCGC 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 273, 676, 900
FokI GGATG 4 cut(s) 68, 644, 860, 970
Fsp4HI GCNGC 3 cut(s) 273, 676, 900
FspBI CTAG 3 cut(s) 335, 935, 981
GluI GCNGC 3 cut(s) 273, 676, 900
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 6 cut(s) 128, 167, 330, 585, 598, 724
HapII CCGG 2 cut(s) 331, 832
HgaI GACGC 3 cut(s) 380, 625, 899
Hin1I GRCGYC 2 cut(s) 372, 617
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HinfI GANTC 5 cut(s) 4, 155, 194, 928, 1112
HpaII CCGG 2 cut(s) 331, 832
HphI GGTGA 3 cut(s) 415, 712, 1176
Hpy166II GTNNAC 4 cut(s) 318, 750, 841, 858
Hpy188I TCNGA 7 cut(s) 37, 199, 262, 358, 612, 973, 1181
Hpy188III TCNNGA 5 cut(s) 152, 670, 818, 1094, 1162
Hpy8I GTNNAC 4 cut(s) 318, 750, 841, 858
Hpy99I CGWCG 3 cut(s) 386, 915, 953
HpyAV CCTTC 4 cut(s) 440, 797, 865, 1163
HpyCH4III ACNGT 2 cut(s) 427, 745
HpyCH4V TGCA 7 cut(s) 41, 294, 626, 678, 750, 766, 858
HpyF10VI GCNNNNNNNGC 6 cut(s) 278, 344, 443, 632, 864, 1025
HpyF3I CTNAG 5 cut(s) 636, 706, 892, 1178, 1266
Hsp92I GRCGYC 2 cut(s) 372, 617
KroI GCCGGC 1 cut(s) 330
KroNI GCCGGC 1 cut(s) 332
Kzo9I GATC 4 cut(s) 666, 718, 1181, 1269
Lsp1109I GCAGC 3 cut(s) 259, 662, 886
LweI GCATC 2 cut(s) 28, 622
MaeI CTAG 3 cut(s) 335, 935, 981
MaeIII GTNAC 1 cut(s) 546
MalI GATC 4 cut(s) 668, 720, 1183, 1271
MboI GATC 4 cut(s) 666, 718, 1181, 1269
MboII GAAGA 7 cut(s) 24, 247, 422, 499, 653, 664, 1132
MhlI GDGCHC 2 cut(s) 752, 860
MluCI AATT 2 cut(s) 1127, 1245
MlyI GAGTC 2 cut(s) 149, 1121
MroNI GCCGGC 1 cut(s) 330
MroXI GAANNNNTTC 1 cut(s) 1089
MseI TTAA 3 cut(s) 69, 111, 1050
MslI CAYNNNNRTG 3 cut(s) 227, 625, 798
MspI CCGG 2 cut(s) 331, 832
MspR9I CCNGG 2 cut(s) 163, 249
Mva1269I GAATGC 1 cut(s) 109
MvaI CCWGG 2 cut(s) 163, 249
MwoI GCNNNNNNNGC 6 cut(s) 278, 344, 443, 632, 864, 1025
NaeI GCCGGC 1 cut(s) 332
NcoI CCATGG 1 cut(s) 696
NdeII GATC 4 cut(s) 666, 718, 1181, 1269
NgoMIV GCCGGC 1 cut(s) 330
NheI GCTAGC 1 cut(s) 334
NlaIV GGNNCC 4 cut(s) 320, 523, 586, 1137
NmeAIII GCCGAG 1 cut(s) 750
NspI RCATGY 2 cut(s) 766, 839
NspV TTCGAA 1 cut(s) 192
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 405
PctI GAATGC 1 cut(s) 109
PdiI GCCGGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 3 cut(s) 4, 194, 928
PkrI GCNGC 3 cut(s) 274, 677, 901
PleI GAGTC 2 cut(s) 149, 1120
PpsI GAGTC 2 cut(s) 149, 1120
PpuMI RGGWCCY 1 cut(s) 146
PshAI GACNNNNGTC 1 cut(s) 533
PsiI TTATAA 1 cut(s) 884
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 2 cut(s) 161, 247
PspGI CCWGG 2 cut(s) 161, 247
PspN4I GGNNCC 4 cut(s) 320, 523, 586, 1137
PspPI GGNCC 7 cut(s) 146, 165, 241, 318, 584, 597, 1136
PspPPI RGGWCCY 1 cut(s) 146
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 3 cut(s) 227, 625, 798
Rsr2I CGGWCCG 1 cut(s) 241
RsrII CGGWCCG 1 cut(s) 241
SaqAI TTAA 3 cut(s) 69, 111, 1050
SatI GCNGC 3 cut(s) 273, 676, 900
Sau3AI GATC 4 cut(s) 666, 718, 1181, 1269
Sau96I GGNCC 7 cut(s) 146, 165, 241, 318, 584, 597, 1136
SchI GAGTC 2 cut(s) 149, 1121
ScrFI CCNGG 2 cut(s) 163, 249
SduI GDGCHC 2 cut(s) 752, 860
SfaNI GCATC 2 cut(s) 28, 622
SfcI CTRYAG 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 192
SinI GGWCC 4 cut(s) 146, 241, 318, 1136
SmiMI CAYNNNNRTG 3 cut(s) 227, 625, 798
Sse9I AATT 2 cut(s) 1127, 1245
SsiI CCGC 5 cut(s) 244, 394, 444, 600, 659
SspI AATATT 2 cut(s) 187, 1222
SspMI CTAG 3 cut(s) 335, 935, 981
StyD4I CCNGG 2 cut(s) 161, 247
StyI CCWWGG 2 cut(s) 696, 1033
TaaI ACNGT 2 cut(s) 427, 745
TasI AATT 2 cut(s) 1127, 1245
TfiI GAWTC 3 cut(s) 4, 194, 928
Tru1I TTAA 3 cut(s) 69, 111, 1050
Tru9I TTAA 3 cut(s) 69, 111, 1050
TscAI CASTG 1 cut(s) 373
TseI GCWGC 3 cut(s) 272, 675, 899
TspDTI ATGAA 5 cut(s) 17, 40, 53, 816, 1133
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 1 cut(s) 373
VneI GTGCAC 2 cut(s) 748, 856
VpaK11BI GGWCC 4 cut(s) 146, 241, 318, 1136
XceI RCATGY 2 cut(s) 766, 839
XmnI GAANNNNTTC 1 cut(s) 1089
XspI CTAG 3 cut(s) 335, 935, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.