MD15G1127200.v1.1

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011257
Physical Location & Seq
Forward (+)
9235613 .. 9236580
968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1127200.v1.1.491

Sequence Viewer

Length: 498 bp
ATGCAGACCGCCGGGTCTGTTATCGGAGACCTCGCACTGGCGGATCTCCAGTGCAACATAGTAAAAGGCCATGCCTGGAGCCACAATGCCCGCCTGGCATGTGTTGCTCAAGTCTCTGATCAATCAACTGACACCACAATCGATGGCCCCCACCACCTTGCCACCACTGAAGTCCACTTCATCGCCGTCCTCCGTGCCACCACTGCCCTCAGCCCGTTCGGAAAAGAACCCAAATTTCAACAAGAAGTGAAGACCTCTGGGCTTATGGGAGGAGGAGATAATAATTACAATATTCATCAAGGCACCATGCGTACTAATGTGGAGCGCAGGTTGCACCAGCTCCTGCTTTCTGTGAGGGACTTTAATGGACCTTACTTTGGGGCCGGCAAGCTGGCAAAGGACACTGTTGGGTATTTTATTCGACACATCGACGGGGATGCATTGTGTACTGAGAGTGAGAAGGAAAGAGTAATAAAATGCTTAGAGGCTGATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

166

Amino Acids

18.01

Weight (kDa)

6.22

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 302
AciI CCGC 3 cut(s) 9, 41, 91
AclWI GGATC 1 cut(s) 51
AcsI RAATTY 1 cut(s) 233
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 2 cut(s) 313, 448
AfiI CCNNNNNNNGG 2 cut(s) 37, 377
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 2 cut(s) 74, 93
AluBI AGCT 2 cut(s) 340, 391
AluI AGCT 2 cut(s) 340, 391
Alw26I GTCTC 2 cut(s) 21, 118
AlwI GGATC 1 cut(s) 51
AlwNI CAGNNNCTG 1 cut(s) 343
AoxI GGCC 3 cut(s) 67, 145, 381
ApoI RAATTY 1 cut(s) 233
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 3 cut(s) 146, 368, 381
AsuC2I CCSGG 1 cut(s) 13
AvaII GGWCC 1 cut(s) 368
BanI GGYRCC 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 257
BbvCI CCTCAGC 1 cut(s) 209
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 170
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 2 cut(s) 76, 95
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 21, 118
BfuAI ACCTGC 1 cut(s) 318
BglI GCCNNNNNGGC 1 cut(s) 95
Bme1390I CCNGG 3 cut(s) 13, 76, 95
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 146, 368, 381
BmiI GGNNCC 4 cut(s) 80, 148, 304, 382
BmrFI CCNGG 3 cut(s) 13, 76, 95
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 257
BpmI CTGGAG 2 cut(s) 32, 97
Bpu10I CCTNAGC 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 13
Bsa29I ATCGAT 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 377
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 2 cut(s) 42, 49
BseBI CCWGG 2 cut(s) 76, 95
BseCI ATCGAT 1 cut(s) 141
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 2 cut(s) 37, 377
BseMII CTCAG 2 cut(s) 223, 441
BseNI ACTGG 2 cut(s) 42, 49
BseRI GAGGAG 2 cut(s) 285, 288
BshFI GGCC 3 cut(s) 69, 147, 383
BshNI GGYRCC 1 cut(s) 302
BshVI ATCGAT 1 cut(s) 141
BsiSI CCGG 2 cut(s) 12, 384
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 2 cut(s) 37, 377
BsmAI GTCTC 2 cut(s) 21, 118
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 3 cut(s) 69, 147, 383
Bso31I GGTCTC 1 cut(s) 21
Bsp143I GATC 2 cut(s) 43, 118
BspACI CCGC 3 cut(s) 9, 41, 91
BspANI GGCC 3 cut(s) 69, 147, 383
BspCNI CTCAG 2 cut(s) 222, 442
BspDI ATCGAT 1 cut(s) 141
BspLI GGNNCC 4 cut(s) 80, 148, 304, 382
BspMI ACCTGC 1 cut(s) 318
BspPI GGATC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 2 cut(s) 42, 49
BssAI RCCGGY 1 cut(s) 383
BssMI GATC 2 cut(s) 43, 118
Bst2UI CCWGG 2 cut(s) 76, 95
Bst4CI ACNGT 1 cut(s) 406
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 4 cut(s) 91, 385, 389, 393
BstDEI CTNAG 3 cut(s) 209, 450, 481
BstF5I GGATG 1 cut(s) 442
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 2 cut(s) 46, 121
BstMAI GTCTC 2 cut(s) 21, 118
BstMBI GATC 2 cut(s) 43, 118
BstMWI GCNNNNNNNGC 4 cut(s) 95, 104, 203, 331
BstNI CCWGG 2 cut(s) 76, 95
BstNSI RCATGY 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 11, 74, 93
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
Bsu15I ATCGAT 1 cut(s) 141
BsuRI GGCC 3 cut(s) 69, 147, 383
BsuTUI ATCGAT 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 442
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 5 cut(s) 35, 56, 165, 201, 402
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 4 cut(s) 91, 385, 389, 393
CaiI CAGNNNCTG 1 cut(s) 343
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 3 cut(s) 146, 368, 381
ClaI ATCGAT 1 cut(s) 141
Csp6I GTAC 2 cut(s) 312, 447
CviAII CATG 3 cut(s) 71, 99, 307
CviJI RGCY 9 cut(s) 69, 81, 147, 213, 262, 340, 383, 391, 488
CviKI_1 RGCY 9 cut(s) 69, 81, 147, 213, 262, 340, 383, 391, 488
CviQI GTAC 2 cut(s) 312, 447
DdeI CTNAG 3 cut(s) 209, 450, 481
DpnI GATC 2 cut(s) 45, 120
DpnII GATC 2 cut(s) 43, 118
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
EciI GGCGGA 1 cut(s) 56
Eco31I GGTCTC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 189
EcoRII CCWGG 2 cut(s) 74, 93
EcoT22I ATGCAT 1 cut(s) 442
FaeI CATG 3 cut(s) 74, 102, 310
FaiI YATR 7 cut(s) 59, 72, 100, 266, 308, 494, 496
FaqI GGGAC 1 cut(s) 371
FatI CATG 3 cut(s) 70, 98, 306
FauI CCCGC 1 cut(s) 98
FbaI TGATCA 1 cut(s) 118
FokI GGATG 1 cut(s) 449
GlaI GCGC 1 cut(s) 326
GsuI CTGGAG 2 cut(s) 32, 97
HaeIII GGCC 3 cut(s) 69, 147, 383
HapII CCGG 2 cut(s) 12, 384
HhaI GCGC 1 cut(s) 327
Hin1II CATG 3 cut(s) 74, 102, 310
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HpaII CCGG 2 cut(s) 12, 384
Hpy166II GTNNAC 2 cut(s) 175, 447
Hpy188I TCNGA 3 cut(s) 26, 118, 221
Hpy8I GTNNAC 2 cut(s) 175, 447
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 1 cut(s) 454
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4V TGCA 4 cut(s) 4, 54, 334, 440
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 104, 203, 331
HpyF3I CTNAG 3 cut(s) 209, 450, 481
Hsp92II CATG 3 cut(s) 74, 102, 310
HspAI GCGC 1 cut(s) 325
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 43, 118
LmnI GCTCC 3 cut(s) 78, 322, 345
LweI GCATC 1 cut(s) 427
MalI GATC 2 cut(s) 45, 120
MboI GATC 2 cut(s) 43, 118
MboII GAAGA 1 cut(s) 262
MflI RGATCY 1 cut(s) 43
MluCI AATT 2 cut(s) 233, 283
MnlI CCTC 8 cut(s) 41, 200, 218, 263, 265, 266, 348, 478
Mph1103I ATGCAT 1 cut(s) 442
MroNI GCCGGC 1 cut(s) 383
MseI TTAA 1 cut(s) 363
MspI CCGG 2 cut(s) 12, 384
MspR9I CCNGG 3 cut(s) 13, 76, 95
MvaI CCWGG 2 cut(s) 76, 95
MwoI GCNNNNNNNGC 4 cut(s) 95, 104, 203, 331
NaeI GCCGGC 1 cut(s) 385
NciI CCSGG 1 cut(s) 13
NdeII GATC 2 cut(s) 43, 118
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 3 cut(s) 74, 102, 310
NlaIV GGNNCC 4 cut(s) 80, 148, 304, 382
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 74, 93
PspGI CCWGG 2 cut(s) 74, 93
PspN4I GGNNCC 4 cut(s) 80, 148, 304, 382
PspPI GGNCC 3 cut(s) 146, 368, 381
PstNI CAGNNNCTG 1 cut(s) 343
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 2 cut(s) 313, 448
RsaNI GTAC 2 cut(s) 312, 447
SaqAI TTAA 1 cut(s) 363
Sau3AI GATC 2 cut(s) 43, 118
Sau96I GGNCC 3 cut(s) 146, 368, 381
ScrFI CCNGG 3 cut(s) 13, 76, 95
SetI ASST 7 cut(s) 33, 159, 257, 332, 342, 373, 393
SfaNI GCATC 1 cut(s) 427
SinI GGWCC 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
Sse9I AATT 2 cut(s) 233, 283
SsiI CCGC 3 cut(s) 9, 41, 91
SspI AATATT 1 cut(s) 292
StyD4I CCNGG 3 cut(s) 11, 74, 93
TaaI ACNGT 1 cut(s) 406
TaqI TCGA 3 cut(s) 141, 421, 429
TasI AATT 2 cut(s) 233, 283
TatI WGTACW 1 cut(s) 446
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 5 cut(s) 42, 56, 172, 208, 409
TspDTI ATGAA 2 cut(s) 169, 284
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 5 cut(s) 42, 56, 172, 208, 409
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.