Rh6AG385000

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011257
Physical Location & Seq
Reverse (-)
58435335 .. 58438562
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG385000.1

Sequence Viewer

Length: 1233 bp
ATGCATGCTGCAGTTTTTCATGTTAAAGATGAGAATGGCAACAAACTTACAGACCAGAATGCCATTAACTATATCCAGCAGGCCATAGGTACAACTACAGGTCCTCCAGACTCCACCAGGGCCAATGCCTATGCCAGCAATATTTTCGAATCCGAAAACCATAGCGAGCATACGACCATCGAAATGACGGGAACGGACCGACCTGGTCTCTTCTCGGAGATATCGGCTGCCCTTGCCGACCTCCAATGCAACATAGTTGAAGCCCATGCGTGGACCCATAACGGTCGGCTAGCTTGTGTTGCTCAAATATCTGACCAATCCACTGACGCCCCAATCGAGGACCCCCACCGCCTTGCCACCATCGAAGACCACCTCATCACCGTCCTTCGAGCCACCACTTCCGTAAGCCCTTGTGGGAAAGAAGCAAACAACCAAGAAGTGAAGACCACAGGGTTTTTAGGAGGAGACTATCAAGGCACCATGACTAATGTCGAAAGGAGGTTACACCAACTAATGCTTTCCATTAGGGACTTTGACGGCCCCAATTTGAGGCCCGCCAGCTCTCCGAGGACGCCATTCGCATGGGATGCTGAGGAAGAGTGTAGGAAGACGGCGGTTTGGATCGAGAGCTGCAAGGAGAAAGGGTATTCCATGGTGAGCTTAGAGTGTAAGGATCGGCCGAGGCTTATGTTTGATACTGTGTGCACTCTAACTGACATGCAGTATGTGATTTTCCATGCTTCCACTCATTGCCATGAAGGATATGCCTTTCAGGAATACTTTATCCGGCATGTTGACGGGGATGTCTTGTGCACAGAGAGCGAGAAGGAACGAGTTATAAAATGCTTAGAGGCTGCTATTGAGCGTCGAGTTTGTGAGGGGATTCGACTAGAGTTGCGTGCCGACGATAGGATGGGATTGCTATCGGATATAACCAGGGTTCTACGAGAGAATGGACTTGCTGTTGTTCGAGCAGATGTAGCAACCCAAGGAGAGAAGTCGACTAACGCTTTCTATGTGAGGGACATTTCAGGGAACGAAGTAGTTCCAGACATGGATTTTGTTGAGTCCATGAAGAGAGAATTGATGGGTCCAAGTCATATAAACCTTCAAGTCAAGAATGAGAAACATCTCAGATCACCAAGCTCAACTGAAAAGTCTCGTTATTTCTCAAATATTGGAGATATTCTAAAATCTCAAATTGGGCGTTTCGCTTTTGCTAAGATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

410

Amino Acids

45.96

Weight (kDa)

5.56

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 58 - 95 1.4e-06 ACT domain
ACT_6 PF13740 60 - 104 2.6e-07 ACT domain
ACT PF01842 296 - 345 2.3e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 839
AasI GACNNNNNNGTC 1 cut(s) 803
AccB1I GGYRCC 1 cut(s) 476
AccI GTMKAC 1 cut(s) 1001
AciI CCGC 3 cut(s) 349, 555, 614
AclWI GGATC 2 cut(s) 629, 681
AcoI YGGCCR 1 cut(s) 677
AcyI GRCGYC 2 cut(s) 327, 572
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 4 cut(s) 270, 337, 549, 909
AgsI TTSAA 2 cut(s) 260, 1112
AjnI CCWGG 3 cut(s) 116, 202, 935
AluBI AGCT 5 cut(s) 293, 561, 630, 660, 1146
AluI AGCT 5 cut(s) 293, 561, 630, 660, 1146
Alw21I GWGCWC 2 cut(s) 707, 815
Alw26I GTCTC 3 cut(s) 212, 459, 1164
Alw44I GTGCAC 2 cut(s) 703, 811
AlwI GGATC 2 cut(s) 629, 681
AoxI GGCC 5 cut(s) 81, 120, 538, 551, 677
ApaLI GTGCAC 2 cut(s) 703, 811
ApeKI GCWGC 4 cut(s) 8, 227, 630, 854
ArsI GACNNNNNNTTYG 2 cut(s) 1043, 1075
Asp700I GAANNNNTTC 1 cut(s) 1044
AspS9I GGNCC 8 cut(s) 101, 120, 196, 273, 340, 539, 552, 1091
AsuHPI GGTGA 3 cut(s) 370, 667, 1131
AsuII TTCGAA 1 cut(s) 147
AsuNHI GCTAGC 1 cut(s) 289
AvaII GGWCC 5 cut(s) 101, 196, 273, 340, 1091
BaeGI GKGCMC 2 cut(s) 707, 815
BanI GGYRCC 1 cut(s) 476
BbsI GAAGAC 3 cut(s) 372, 449, 614
Bbv12I GWGCWC 2 cut(s) 707, 815
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 214, 617, 841
BccI CCATC 4 cut(s) 185, 368, 907, 1081
BceAI ACGGC 2 cut(s) 553, 627
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 3 cut(s) 118, 204, 937
BcoDI GTCTC 3 cut(s) 212, 459, 1164
BfaI CTAG 2 cut(s) 290, 890
BfmI CTRYAG 2 cut(s) 9, 96
BisI GCNGC 4 cut(s) 9, 228, 631, 855
BlsI GCNGC 4 cut(s) 10, 229, 632, 856
Bme1390I CCNGG 3 cut(s) 118, 204, 937
Bme18I GGWCC 5 cut(s) 101, 196, 273, 340, 1091
BmgT120I GGNCC 8 cut(s) 101, 120, 196, 273, 340, 539, 552, 1091
BmiI GGNNCC 5 cut(s) 275, 342, 478, 541, 1092
BmrFI CCNGG 3 cut(s) 118, 204, 937
BmsI GCATC 1 cut(s) 577
BmtI GCTAGC 1 cut(s) 293
BoxI GACNNNNGTC 1 cut(s) 488
BpiI GAAGAC 3 cut(s) 372, 449, 614
BpmI CTGGAG 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 922
BsaHI GRCGYC 2 cut(s) 327, 572
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 6 cut(s) 117, 566, 651, 680, 936, 988
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 629, 659
Bsc4I CCNNNNNNNGG 4 cut(s) 270, 337, 549, 909
Bse3DI GCAATG 1 cut(s) 748
Bse8I GATNNNNATC 1 cut(s) 922
BseBI CCWGG 3 cut(s) 118, 204, 937
BseDI CCNNGG 6 cut(s) 117, 566, 651, 680, 936, 988
BseGI GGATG 3 cut(s) 592, 808, 918
BseJI GATNNNNATC 1 cut(s) 922
BseLI CCNNNNNNNGG 4 cut(s) 270, 337, 549, 909
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 2 cut(s) 582, 1147
BseRI GAGGAG 1 cut(s) 477
BseSI GKGCMC 2 cut(s) 707, 815
BseX3I CGGCCG 1 cut(s) 677
BseXI GCAGC 3 cut(s) 214, 617, 841
Bsh1285I CGRYCG 2 cut(s) 286, 680
BshFI GGCC 5 cut(s) 83, 122, 540, 553, 679
BshNI GGYRCC 1 cut(s) 476
BsiEI CGRYCG 2 cut(s) 286, 680
BsiHKAI GWGCWC 2 cut(s) 707, 815
BsiSI CCGG 1 cut(s) 787
BslFI GGGAC 2 cut(s) 542, 1037
BslI CCNNNNNNNGG 4 cut(s) 270, 337, 549, 909
BsmAI GTCTC 3 cut(s) 212, 459, 1164
BsmFI GGGAC 2 cut(s) 542, 1037
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 5 cut(s) 83, 122, 540, 553, 679
Bso31I GGTCTC 1 cut(s) 212
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 707, 815
Bsp143I GATC 4 cut(s) 621, 673, 1136, 1224
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 3 cut(s) 349, 555, 614
BspANI GGCC 5 cut(s) 83, 122, 540, 553, 679
BspCNI CTCAG 2 cut(s) 583, 1146
BspLI GGNNCC 5 cut(s) 275, 342, 478, 541, 1092
BspMAI CTGCAG 1 cut(s) 13
BspOI GCTAGC 1 cut(s) 293
BspPI GGATC 2 cut(s) 629, 681
BspT104I TTCGAA 1 cut(s) 147
BspT107I GGYRCC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 748
BssECI CCNNGG 6 cut(s) 117, 566, 651, 680, 936, 988
BssMI GATC 4 cut(s) 621, 673, 1136, 1224
BssNI GRCGYC 2 cut(s) 327, 572
BssT1I CCWWGG 2 cut(s) 651, 988
Bst2UI CCWGG 3 cut(s) 118, 204, 937
Bst4CI ACNGT 3 cut(s) 284, 382, 700
Bst6I CTCTTC 3 cut(s) 215, 591, 1070
BstACI GRCGYC 2 cut(s) 327, 572
BstAPI GCANNNNNTGC 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 8 cut(s) 6, 81, 136, 167, 291, 555, 559, 900
BstDEI CTNAG 5 cut(s) 591, 661, 847, 1133, 1221
BstDSI CCRYGG 1 cut(s) 651
BstF5I GGATG 3 cut(s) 592, 808, 918
BstKTI GATC 4 cut(s) 624, 676, 1139, 1227
BstMAI GTCTC 3 cut(s) 212, 459, 1164
BstMBI GATC 4 cut(s) 621, 673, 1136, 1224
BstMCI CGRYCG 2 cut(s) 286, 680
BstMWI GCNNNNNNNGC 5 cut(s) 233, 299, 587, 819, 980
BstNI CCWGG 3 cut(s) 118, 204, 937
BstNSI RCATGY 3 cut(s) 8, 721, 794
BstPAI GACNNNNGTC 1 cut(s) 488
BstSCI CCNGG 3 cut(s) 116, 202, 935
BstSFI CTRYAG 2 cut(s) 9, 96
BstSLI GKGCMC 2 cut(s) 707, 815
BstV1I GCAGC 3 cut(s) 214, 617, 841
BstV2I GAAGAC 3 cut(s) 372, 449, 614
BstXI CCANNNNNNTGG 1 cut(s) 582
BstZI CGGCCG 1 cut(s) 677
BsuRI GGCC 5 cut(s) 83, 122, 540, 553, 679
BtgI CCRYGG 1 cut(s) 651
BtsCI GGATG 3 cut(s) 592, 808, 918
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 8 cut(s) 6, 81, 136, 167, 291, 555, 559, 900
Cfr13I GGNCC 8 cut(s) 101, 120, 196, 273, 340, 539, 552, 1091
CpoI CGGWCCG 1 cut(s) 196
CseI GACGC 3 cut(s) 335, 580, 854
CsiI ACCWGGT 1 cut(s) 202
Csp6I GTAC 1 cut(s) 90
CspI CGGWCCG 1 cut(s) 196
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 5 cut(s) 591, 661, 847, 1133, 1221
DpnI GATC 4 cut(s) 623, 675, 1138, 1226
DpnII GATC 4 cut(s) 621, 673, 1136, 1224
DrdI GACNNNNNNGTC 1 cut(s) 803
DseDI GACNNNNNNGTC 1 cut(s) 803
EaeI YGGCCR 1 cut(s) 677
EagI CGGCCG 1 cut(s) 677
Eam1104I CTCTTC 3 cut(s) 215, 591, 1070
EarI CTCTTC 3 cut(s) 215, 591, 1070
EclXI CGGCCG 1 cut(s) 677
Eco130I CCWWGG 2 cut(s) 651, 988
Eco31I GGTCTC 1 cut(s) 212
Eco32I GATATC 1 cut(s) 222
Eco47I GGWCC 5 cut(s) 101, 196, 273, 340, 1091
Eco52I CGGCCG 1 cut(s) 677
EcoO109I RGGNCCY 2 cut(s) 101, 340
EcoRII CCWGG 3 cut(s) 116, 202, 935
EcoRV GATATC 1 cut(s) 222
EcoT14I CCWWGG 2 cut(s) 651, 988
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 651, 988
FaqI GGGAC 2 cut(s) 542, 1037
FauI CCCGC 1 cut(s) 562
FblI GTMKAC 1 cut(s) 1001
Fnu4HI GCNGC 4 cut(s) 9, 228, 631, 855
FokI GGATG 3 cut(s) 599, 815, 925
Fsp4HI GCNGC 4 cut(s) 9, 228, 631, 855
FspBI CTAG 2 cut(s) 290, 890
GluI GCNGC 4 cut(s) 9, 228, 631, 855
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 5 cut(s) 83, 122, 540, 553, 679
HapII CCGG 1 cut(s) 787
HgaI GACGC 3 cut(s) 335, 580, 854
Hin1I GRCGYC 2 cut(s) 327, 572
HincII GTYRAC 2 cut(s) 796, 1002
HindII GTYRAC 2 cut(s) 796, 1002
HinfI GANTC 4 cut(s) 110, 149, 883, 1067
HpaII CCGG 1 cut(s) 787
HphI GGTGA 3 cut(s) 370, 667, 1131
Hpy166II GTNNAC 5 cut(s) 273, 705, 796, 813, 1002
Hpy188I TCNGA 6 cut(s) 154, 217, 313, 567, 928, 1136
Hpy188III TCNNGA 5 cut(s) 107, 625, 773, 1049, 1117
Hpy8I GTNNAC 5 cut(s) 273, 705, 796, 813, 1002
Hpy99I CGWCG 2 cut(s) 870, 908
HpyAV CCTTC 4 cut(s) 395, 752, 820, 1118
HpyCH4III ACNGT 3 cut(s) 284, 382, 700
HpyCH4V TGCA 7 cut(s) 4, 11, 249, 633, 705, 721, 813
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 299, 587, 819, 980
HpyF3I CTNAG 5 cut(s) 591, 661, 847, 1133, 1221
Hsp92I GRCGYC 2 cut(s) 327, 572
Kzo9I GATC 4 cut(s) 621, 673, 1136, 1224
Lsp1109I GCAGC 3 cut(s) 214, 617, 841
LweI GCATC 1 cut(s) 577
MabI ACCWGGT 1 cut(s) 202
MaeI CTAG 2 cut(s) 290, 890
MaeIII GTNAC 1 cut(s) 501
MalI GATC 4 cut(s) 623, 675, 1138, 1226
MboI GATC 4 cut(s) 621, 673, 1136, 1224
MboII GAAGA 6 cut(s) 202, 377, 454, 608, 619, 1087
MhlI GDGCHC 2 cut(s) 707, 815
MluCI AATT 3 cut(s) 544, 1082, 1200
MlyI GAGTC 2 cut(s) 104, 1076
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 1044
MseI TTAA 2 cut(s) 24, 66
MslI CAYNNNNRTG 3 cut(s) 182, 580, 753
MspI CCGG 1 cut(s) 787
MspR9I CCNGG 3 cut(s) 118, 204, 937
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 3 cut(s) 118, 204, 937
MwoI GCNNNNNNNGC 5 cut(s) 233, 299, 587, 819, 980
NcoI CCATGG 1 cut(s) 651
NdeII GATC 4 cut(s) 621, 673, 1136, 1224
NheI GCTAGC 1 cut(s) 289
NlaIV GGNNCC 5 cut(s) 275, 342, 478, 541, 1092
NmeAIII GCCGAG 1 cut(s) 705
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 8, 721, 794
NspV TTCGAA 1 cut(s) 147
PaeI GCATGC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 1044
PfeI GAWTC 2 cut(s) 149, 883
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 4 cut(s) 10, 229, 632, 856
PleI GAGTC 2 cut(s) 104, 1075
PpsI GAGTC 2 cut(s) 104, 1075
PpuMI RGGWCCY 2 cut(s) 101, 340
PshAI GACNNNNGTC 1 cut(s) 488
PsiI TTATAA 1 cut(s) 839
Psp5II RGGWCCY 2 cut(s) 101, 340
Psp6I CCWGG 3 cut(s) 116, 202, 935
PspGI CCWGG 3 cut(s) 116, 202, 935
PspN4I GGNNCC 5 cut(s) 275, 342, 478, 541, 1092
PspPI GGNCC 8 cut(s) 101, 120, 196, 273, 340, 539, 552, 1091
PspPPI RGGWCCY 2 cut(s) 101, 340
PstI CTGCAG 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 204
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
RseI CAYNNNNRTG 3 cut(s) 182, 580, 753
Rsr2I CGGWCCG 1 cut(s) 196
RsrII CGGWCCG 1 cut(s) 196
SalI GTCGAC 1 cut(s) 1000
SaqAI TTAA 2 cut(s) 24, 66
SatI GCNGC 4 cut(s) 9, 228, 631, 855
Sau3AI GATC 4 cut(s) 621, 673, 1136, 1224
Sau96I GGNCC 8 cut(s) 101, 120, 196, 273, 340, 539, 552, 1091
SchI GAGTC 2 cut(s) 104, 1076
ScrFI CCNGG 3 cut(s) 118, 204, 937
SduI GDGCHC 2 cut(s) 707, 815
SexAI ACCWGGT 1 cut(s) 202
SfaNI GCATC 1 cut(s) 577
SfcI CTRYAG 2 cut(s) 9, 96
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 5 cut(s) 101, 196, 273, 340, 1091
SmiMI CAYNNNNRTG 3 cut(s) 182, 580, 753
SphI GCATGC 1 cut(s) 8
Sse9I AATT 3 cut(s) 544, 1082, 1200
SsiI CCGC 3 cut(s) 349, 555, 614
SspI AATATT 2 cut(s) 142, 1177
SspMI CTAG 2 cut(s) 290, 890
StyD4I CCNGG 3 cut(s) 116, 202, 935
StyI CCWWGG 2 cut(s) 651, 988
TaaI ACNGT 3 cut(s) 284, 382, 700
TaqII GACCGA 1 cut(s) 213
TasI AATT 3 cut(s) 544, 1082, 1200
TfiI GAWTC 2 cut(s) 149, 883
Tru1I TTAA 2 cut(s) 24, 66
Tru9I TTAA 2 cut(s) 24, 66
TscAI CASTG 1 cut(s) 328
TseI GCWGC 4 cut(s) 8, 227, 630, 854
TspDTI ATGAA 3 cut(s) 8, 771, 1088
TspGWI ACGGA 2 cut(s) 209, 391
TspRI CASTG 1 cut(s) 328
Tth111I GACNNNGTC 1 cut(s) 204
VneI GTGCAC 2 cut(s) 703, 811
VpaK11BI GGWCC 5 cut(s) 101, 196, 273, 340, 1091
XceI RCATGY 3 cut(s) 8, 721, 794
XmiI GTMKAC 1 cut(s) 1001
XmnI GAANNNNTTC 1 cut(s) 1044
XspI CTAG 2 cut(s) 290, 890
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.