Rorug06G0274200

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011257
Physical Location & Seq
Forward (+)
42123185 .. 42127852
4668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0274200.1

Sequence Viewer

Length: 645 bp
ATGTATGAGTTGTTGATCCCTGTTGTGATTGCTGAGGCTGTTGTGGCATCTCTTATGTTGCTCAAGATTGGGCCTTTGAGAGAGCTTGTGTTGAAGATTCTGGATCAGCTCAAAGTGGGCAGAGGCCCAGTCACTCTGATTGGCATTGCTGGCACCATGGGTGTGATTCTCCTATCAAGATTCATTAGCATTGTCAAGATGCAGAACAAAGGTGAAAAGCTTGGGACTATGTCACCCATGGATCAGGTTCTGTACAGAACCCACATCCTTGAAGCGTCATTAATGTCTTTTGCTGTATTTCTTGGATTCATTATTGATCGAACACACGATTATCTTACAAAGCTTGATGGATTGACGAGTACAAAAGAAGAAGTTGACAGGCTACAGAAAGAAAAGGCGCTGCTTACAGAAAAGGAAGAGAAATCTTCCAAGGAAATCAAGCAGCTGCAAGAAAAAATTGCAACTCTATCGGAGGATTTGAAGAAGCTGAAGTTGGAGTGTGCGCAGAAAGATAAAGCTGTTGAAACTGCTGAATTCCATGTCACTTCCCTCCAGAAACAAGCTGCAGATCTACTTCTTGAGTATGACCATTTGTTAGAAGACAACCAAAAACTTCAAGCTCAGTTTGCAGGACAGAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

214

Amino Acids

24.11

Weight (kDa)

6.92

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 152
AclWI GGATC 3 cut(s) 10, 111, 249
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 1 cut(s) 509
AfaI GTAC 2 cut(s) 254, 361
AgsI TTSAA 5 cut(s) 94, 272, 481, 524, 617
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 9 cut(s) 85, 109, 220, 343, 445, 487, 518, 563, 620
AluI AGCT 9 cut(s) 85, 109, 220, 343, 445, 487, 518, 563, 620
AlwI GGATC 3 cut(s) 10, 111, 249
AlwNI CAGNNNCTG 1 cut(s) 250
AoxI GGCC 2 cut(s) 71, 124
ApeKI GCWGC 4 cut(s) 400, 442, 445, 563
ApoI RAATTY 1 cut(s) 533
AseI ATTAAT 1 cut(s) 281
AspLEI GCGC 2 cut(s) 400, 505
AspS9I GGNCC 2 cut(s) 71, 125
AsuHPI GGTGA 2 cut(s) 224, 225
BanI GGYRCC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 606
BbvCI CCTCAGC 1 cut(s) 33
BbvI GCAGC 4 cut(s) 387, 432, 454, 550
BccI CCATC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 450, 484
BfmI CTRYAG 2 cut(s) 383, 564
BfoI RGCGCY 1 cut(s) 401
BglII AGATCT 1 cut(s) 568
BisI GCNGC 4 cut(s) 401, 443, 446, 564
BlsI GCNGC 4 cut(s) 402, 444, 447, 565
BmgT120I GGNCC 2 cut(s) 71, 125
BmiI GGNNCC 1 cut(s) 154
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 2 cut(s) 56, 189
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 606
BpmI CTGGAG 1 cut(s) 536
Bpu10I CCTNAGC 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 47, 599
BsaJI CCNNGG 3 cut(s) 156, 237, 429
Bse1I ACTGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 144
BseDI CCNNGG 3 cut(s) 156, 237, 429
BseGI GGATG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 24, 635
BseNI ACTGG 1 cut(s) 128
BseXI GCAGC 4 cut(s) 387, 432, 454, 550
BshFI GGCC 2 cut(s) 73, 126
BshNI GGYRCC 1 cut(s) 152
BslFI GGGAC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 2 cut(s) 73, 126
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 5 cut(s) 15, 103, 241, 316, 568
Bsp19I CCATGG 2 cut(s) 156, 237
BspANI GGCC 2 cut(s) 73, 126
BspCNI CTCAG 2 cut(s) 25, 634
BspLI GGNNCC 1 cut(s) 154
BspMAI CTGCAG 1 cut(s) 568
BspPI GGATC 3 cut(s) 10, 111, 249
BspT107I GGYRCC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 3 cut(s) 156, 237, 429
BssMI GATC 5 cut(s) 15, 103, 241, 316, 568
BssT1I CCWWGG 3 cut(s) 156, 237, 429
Bst6I CTCTTC 1 cut(s) 411
BstAUI TGTACA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 2 cut(s) 33, 621
BstDSI CCRYGG 2 cut(s) 156, 237
BstF5I GGATG 1 cut(s) 264
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 400, 505
BstKTI GATC 5 cut(s) 18, 106, 244, 319, 571
BstMBI GATC 5 cut(s) 15, 103, 241, 316, 568
BstMWI GCNNNNNNNGC 3 cut(s) 44, 150, 626
BstSFI CTRYAG 2 cut(s) 383, 564
BstV1I GCAGC 4 cut(s) 387, 432, 454, 550
BstV2I GAAGAC 1 cut(s) 606
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 2 cut(s) 73, 126
BtgI CCRYGG 2 cut(s) 156, 237
BtsCI GGATG 1 cut(s) 264
Cac8I GCNNGC 1 cut(s) 151
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 2 cut(s) 400, 505
Cfr13I GGNCC 2 cut(s) 71, 125
CseI GACGC 1 cut(s) 264
Csp6I GTAC 2 cut(s) 253, 360
CviAII CATG 3 cut(s) 157, 238, 539
CviQI GTAC 2 cut(s) 253, 360
DdeI CTNAG 2 cut(s) 33, 621
DpnI GATC 5 cut(s) 17, 105, 243, 318, 570
DpnII GATC 5 cut(s) 15, 103, 241, 316, 568
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 3 cut(s) 156, 237, 429
Eco57I CTGAAG 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 533
EcoT14I CCWWGG 3 cut(s) 156, 237, 429
ErhI CCWWGG 3 cut(s) 156, 237, 429
FaeI CATG 3 cut(s) 160, 241, 542
FaiI YATR 7 cut(s) 6, 56, 158, 230, 239, 540, 585
FaqI GGGAC 1 cut(s) 238
FatI CATG 3 cut(s) 156, 237, 538
Fnu4HI GCNGC 4 cut(s) 401, 443, 446, 564
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 4 cut(s) 401, 443, 446, 564
FspI TGCGCA 1 cut(s) 504
GlaI GCGC 2 cut(s) 399, 504
GluI GCNGC 4 cut(s) 401, 443, 446, 564
GsuI CTGGAG 1 cut(s) 536
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 2 cut(s) 73, 126
HgaI GACGC 1 cut(s) 264
HhaI GCGC 2 cut(s) 400, 505
Hin1II CATG 3 cut(s) 160, 241, 542
Hin6I GCGC 2 cut(s) 398, 503
HinP1I GCGC 2 cut(s) 398, 503
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 218, 341
HinfI GANTC 4 cut(s) 97, 166, 180, 306
HphI GGTGA 2 cut(s) 224, 225
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 2 cut(s) 138, 472
Hpy188III TCNNGA 6 cut(s) 64, 101, 177, 196, 553, 578
Hpy8I GTNNAC 1 cut(s) 376
HpyCH4V TGCA 5 cut(s) 202, 448, 461, 566, 629
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 150, 626
HpyF3I CTNAG 2 cut(s) 33, 621
Hsp92II CATG 3 cut(s) 160, 241, 542
HspAI GCGC 2 cut(s) 398, 503
Kzo9I GATC 5 cut(s) 15, 103, 241, 316, 568
LpnPI CCDG 8 cut(s) 33, 86, 135, 141, 230, 364, 566, 615
Lsp1109I GCAGC 4 cut(s) 387, 432, 454, 550
LweI GCATC 2 cut(s) 56, 189
MaeIII GTNAC 3 cut(s) 130, 231, 541
MalI GATC 5 cut(s) 17, 105, 243, 318, 570
MboI GATC 5 cut(s) 15, 103, 241, 316, 568
MboII GAAGA 6 cut(s) 106, 380, 417, 428, 493, 611
MflI RGATCY 1 cut(s) 568
MluCI AATT 2 cut(s) 456, 533
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 5 cut(s) 28, 116, 466, 560, 630
MseI TTAA 1 cut(s) 281
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 445
MwoI GCNNNNNNNGC 3 cut(s) 44, 150, 626
NcoI CCATGG 2 cut(s) 156, 237
NdeII GATC 5 cut(s) 15, 103, 241, 316, 568
NlaIII CATG 3 cut(s) 160, 241, 542
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 3 cut(s) 130, 231, 541
NsbI TGCGCA 1 cut(s) 504
PfeI GAWTC 4 cut(s) 97, 166, 180, 306
PflFI GACNNNGTC 1 cut(s) 229
PkrI GCNGC 4 cut(s) 402, 444, 447, 565
PshBI ATTAAT 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 2 cut(s) 71, 125
PstI CTGCAG 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 1 cut(s) 568
PsyI GACNNNGTC 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 2 cut(s) 254, 361
RsaNI GTAC 2 cut(s) 253, 360
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 4 cut(s) 401, 443, 446, 564
Sau3AI GATC 5 cut(s) 15, 103, 241, 316, 568
Sau96I GGNCC 2 cut(s) 71, 125
SfaNI GCATC 2 cut(s) 56, 189
SfcI CTRYAG 2 cut(s) 383, 564
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 2 cut(s) 62, 578
SmoI CTYRAG 2 cut(s) 62, 578
Sse9I AATT 2 cut(s) 456, 533
StyI CCWWGG 3 cut(s) 156, 237, 429
TaqI TCGA 1 cut(s) 319
TasI AATT 2 cut(s) 456, 533
TatI WGTACW 2 cut(s) 252, 359
TfiI GAWTC 4 cut(s) 97, 166, 180, 306
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TseFI GTSAC 3 cut(s) 130, 231, 541
TseI GCWGC 4 cut(s) 400, 442, 445, 563
Tsp45I GTSAC 3 cut(s) 130, 231, 541
TspDTI ATGAA 2 cut(s) 172, 298
Tth111I GACNNNGTC 1 cut(s) 229
VspI ATTAAT 1 cut(s) 281
XapI RAATTY 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.