MD08G1156600.v1.1

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011257
Physical Location & Seq
Forward (+)
17430126 .. 17432489
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1156600.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGTGCAGCATGAAGAAAGTTTGTTGGCCTTACTTTGATCCTGACTTTGATAATCTCCCAGAGAGGATATATGGCCCTGCTTGTCAAGTATGCATCGACAACGAAGGAATGGAAGATTGCACAGTAGTAAAGGTGGATAGCGTGAACAAGGAAGGGCTTCTCCTGGAAGTGGTGCAAGTTTTGACAGACATGAACCTCACCATCACTAAAAGTTACATTTCTTCTGATGCTGGATGGTTCATGGATGTTTTTCATGTGAAAGATGAACATGGCAACAAAGTTACAGACCAGAATGTCCTTAACTATATTCAACAGGCCATAGGTACAGCCACAGGCCCCCCAGACTTGGCCAAGTCCTACGCCAACAACAACCCTATTTTATTCGAACCCGAAAACCCTATTGAACACACCAGTGAAAATCCTAGCGAGCACACTACCATTGAAATGACCGGTACAGACCGGCCCGGTTTGTTTTCAGAGATCTCCGCTGCCCTGGCGGATCTCCAATGCAACATAGTAGAAGCGCATGCATGGAGCCACAATGCTTGCCTGGCATGTGTTGCTCAAATATCTGATACATCTACCGACACCCCAATCGACGACCCCCGACGCATTGCCACCATAGAAGACCACCTAATCACCGTCCTCCGTGCCACCACAGCCCTCAGCCCGTTTGGGAAGGAACCCAATTGTCAACAAGAAGTGAAGACCTCTGGGCTTATGGGAGGAGGAGATAGTAATCATAATATTCACCAAGGCAGCATGAGTACTAATGTGGACCGTAGGTTGCACCAGCTCATGCTCTCTGTGAGGGACTTTGATGGGCCTCACTTGAGGCCTGCAAGCTCGCCAAGGACGCCCTTGGGGTTGGATAGTGAGGAGGAGAGTCGGAAGACAGTGGTTTGGATTGAGAGTTGTAAGGAAAAAGGGTATTCAATGGTGACTATCGAGTGCAAGGATCGGCCGAGGCTCATGTTTGATACTGTGTGCACTCTCACTGACATGCAATATGTAATTTTCCATGCTTCTGCTAGTGCCCAAGAAGGTTATTCCTTTCAGGAGTATTTTATTCGACACATCGATGGGGATGCCCTGTGTACTGAAAGTGAGAAGGAAAGAGTAATAAAATGCTTAGAGGCTGCTATTGAGCGTCGGGTTTGTGAGGGTGTTCGGCTAGAACTATGCGCAGACAATAGGATAGGGTTGCTCTCGGATATAACTAGGGTTCTACGGGAGAATGGACTTGCGGTTGTTCGAGCAGATGTAGCAACGCAAGGAAAAAAGGCCGTAAACGCCTTCTACGTCAGGGATATTTCAGGCAAAGAAGTAGTAGACATGGATTTGATCGAGTCGATGAAGAGAGAATTAGTGGGTGAAAATCCAATAGATTTTCAAGTCAAGAATGACACATGGATGAGACCGAACTCACTTGAAAAAGGGTCGTCCCCTCTCTCTAATTTTGGAGACATGCTAAAATCTCAGATTGAGCGATTTTCGTACAAGTTTGTCAAGATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

507

Amino Acids

56.59

Weight (kDa)

4.97

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 37 - 107 1.4e-06 Plant bHLH transcription factor, ACT-like domain
ACT PF01842 145 - 179 5.7e-06 ACT domain
ACT_6 PF13740 147 - 191 1.8e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc16I TGCGCA 1 cut(s) 1186
AccI GTMKAC 1 cut(s) 1332
AciI CCGC 3 cut(s) 486, 497, 1247
AclWI GGATC 3 cut(s) 32, 507, 966
AcoI YGGCCR 2 cut(s) 348, 962
AcyI GRCGYC 1 cut(s) 857
AfaI GTAC 5 cut(s) 325, 454, 769, 1099, 1499
AfiI CCNNNNNNNGG 2 cut(s) 169, 346
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 6 cut(s) 311, 404, 443, 936, 1394, 1433
AjnI CCWGG 3 cut(s) 162, 492, 549
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 2 cut(s) 796, 846
AluI AGCT 2 cut(s) 796, 846
Alw21I GWGCWC 2 cut(s) 432, 992
Alw26I GTCTC 2 cut(s) 1411, 1458
Alw44I GTGCAC 1 cut(s) 988
AlwI GGATC 3 cut(s) 32, 507, 966
ApaLI GTGCAC 1 cut(s) 988
ApeKI GCWGC 4 cut(s) 6, 488, 759, 1139
AsiGI ACCGGT 1 cut(s) 449
Asp700I GAANNNNTTC 1 cut(s) 156
AspLEI GCGC 2 cut(s) 526, 1187
AspS9I GGNCC 5 cut(s) 74, 335, 462, 778, 824
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 5 cut(s) 190, 631, 743, 952, 1385
AsuII TTCGAA 1 cut(s) 384
AvaII GGWCC 1 cut(s) 778
BaeGI GKGCMC 2 cut(s) 992, 1039
BalI TGGCCA 1 cut(s) 350
BbsI GAAGAC 3 cut(s) 633, 713, 899
Bbv12I GWGCWC 2 cut(s) 432, 992
BbvCI CCTCAGC 1 cut(s) 665
BbvI GCAGC 4 cut(s) 18, 475, 771, 1126
BccI CCATC 4 cut(s) 209, 228, 815, 1076
BceAI ACGGC 1 cut(s) 1271
BcgI CGANNNNNNTGC 2 cut(s) 1070, 1104
BciT130I CCWGG 3 cut(s) 164, 494, 551
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 2 cut(s) 1411, 1458
BfaI CTAG 4 cut(s) 423, 1032, 1175, 1221
BglII AGATCT 1 cut(s) 480
BisI GCNGC 4 cut(s) 7, 489, 760, 1140
BlsI GCNGC 4 cut(s) 8, 490, 761, 1141
BmcAI AGTACT 1 cut(s) 769
Bme1390I CCNGG 4 cut(s) 164, 465, 494, 551
Bme18I GGWCC 1 cut(s) 778
BmgT120I GGNCC 5 cut(s) 74, 335, 462, 778, 824
BmiI GGNNCC 3 cut(s) 337, 536, 684
BmrFI CCNGG 4 cut(s) 164, 465, 494, 551
BmsI GCATC 3 cut(s) 102, 217, 1078
BpiI GAAGAC 3 cut(s) 633, 713, 899
Bpu10I CCTNAGC 1 cut(s) 665
Bpu14I TTCGAA 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 853
BpuMI CCSGG 1 cut(s) 465
Bsa29I ATCGAT 1 cut(s) 1080
BsaBI GATNNNNATC 1 cut(s) 738
BsaHI GRCGYC 1 cut(s) 857
BsaI GGTCTC 1 cut(s) 1411
BsaJI CCNNGG 5 cut(s) 492, 754, 851, 861, 965
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 346
Bse118I RCCGGY 2 cut(s) 449, 459
Bse1I ACTGG 1 cut(s) 411
Bse3DI GCAATG 1 cut(s) 612
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 3 cut(s) 164, 494, 551
BseCI ATCGAT 1 cut(s) 1080
BseDI CCNNGG 5 cut(s) 492, 754, 851, 861, 965
BseGI GGATG 4 cut(s) 239, 250, 1093, 1419
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 2 cut(s) 169, 346
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 2 cut(s) 679, 1493
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 4 cut(s) 741, 744, 893, 896
BseSI GKGCMC 2 cut(s) 992, 1039
BseX3I CGGCCG 1 cut(s) 962
BseXI GCAGC 4 cut(s) 18, 475, 771, 1126
BsgI GTGCAG 1 cut(s) 25
Bsh1285I CGRYCG 1 cut(s) 965
BshTI ACCGGT 1 cut(s) 449
BshVI ATCGAT 1 cut(s) 1080
BsiEI CGRYCG 1 cut(s) 965
BsiHKAI GWGCWC 2 cut(s) 432, 992
BsiSI CCGG 3 cut(s) 450, 460, 465
BslFI GGGAC 2 cut(s) 827, 1429
BslI CCNNNNNNNGG 2 cut(s) 169, 346
BsmAI GTCTC 2 cut(s) 1411, 1458
BsmFI GGGAC 2 cut(s) 827, 1429
Bso31I GGTCTC 1 cut(s) 1411
Bsp119I TTCGAA 1 cut(s) 384
Bsp1286I GDGCHC 3 cut(s) 432, 992, 1039
Bsp143I GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
BspACI CCGC 3 cut(s) 486, 497, 1247
BspCNI CTCAG 2 cut(s) 678, 1492
BspDI ATCGAT 1 cut(s) 1080
BspLI GGNNCC 3 cut(s) 337, 536, 684
BspPI GGATC 3 cut(s) 32, 507, 966
BspT104I TTCGAA 1 cut(s) 384
BspTNI GGTCTC 1 cut(s) 1411
BsrDI GCAATG 1 cut(s) 612
BsrFI RCCGGY 2 cut(s) 449, 459
BsrI ACTGG 1 cut(s) 411
BssAI RCCGGY 2 cut(s) 449, 459
BssECI CCNNGG 5 cut(s) 492, 754, 851, 861, 965
BssMI GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
BssNI GRCGYC 1 cut(s) 857
BssT1I CCWWGG 3 cut(s) 754, 851, 861
Bst2UI CCWGG 3 cut(s) 164, 494, 551
Bst4CI ACNGT 5 cut(s) 124, 643, 782, 898, 985
Bst6I CTCTTC 1 cut(s) 1352
BstACI GRCGYC 1 cut(s) 857
BstAPI GCANNNNNTGC 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 6 cut(s) 428, 528, 547, 840, 844, 848
BstDEI CTNAG 3 cut(s) 665, 1132, 1479
BstF5I GGATG 4 cut(s) 239, 250, 1093, 1419
BstHHI GCGC 2 cut(s) 526, 1187
BstKTI GATC 6 cut(s) 40, 483, 502, 961, 1347, 1515
BstMAI GTCTC 2 cut(s) 1411, 1458
BstMBI GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
BstMCI CGRYCG 1 cut(s) 965
BstMWI GCNNNNNNNGC 7 cut(s) 494, 551, 560, 659, 856, 1265, 1292
BstNI CCWGG 3 cut(s) 164, 494, 551
BstNSI RCATGY 4 cut(s) 530, 558, 1006, 1471
BstSCI CCNGG 4 cut(s) 162, 463, 492, 549
BstSLI GKGCMC 2 cut(s) 992, 1039
BstV1I GCAGC 4 cut(s) 18, 475, 771, 1126
BstV2I GAAGAC 3 cut(s) 633, 713, 899
BstX2I RGATCY 2 cut(s) 480, 499
BstYI RGATCY 2 cut(s) 480, 499
BstZI CGGCCG 1 cut(s) 962
Bsu15I ATCGAT 1 cut(s) 1080
BsuTUI ATCGAT 1 cut(s) 1080
BtsCI GGATG 4 cut(s) 239, 250, 1093, 1419
BtsIMutI CAGTG 3 cut(s) 418, 903, 996
Cac8I GCNNGC 6 cut(s) 428, 528, 547, 840, 844, 848
CfoI GCGC 2 cut(s) 526, 1187
Cfr10I RCCGGY 2 cut(s) 449, 459
Cfr13I GGNCC 5 cut(s) 74, 335, 462, 778, 824
ClaI ATCGAT 1 cut(s) 1080
CseI GACGC 3 cut(s) 618, 865, 1139
Csp6I GTAC 5 cut(s) 324, 453, 768, 1098, 1498
CspAI ACCGGT 1 cut(s) 449
CspCI CAANNNNNGTGG 2 cut(s) 527, 562
CviQI GTAC 5 cut(s) 324, 453, 768, 1098, 1498
DdeI CTNAG 3 cut(s) 665, 1132, 1479
DpnI GATC 6 cut(s) 39, 482, 501, 960, 1346, 1514
DpnII GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 2 cut(s) 348, 962
EagI CGGCCG 1 cut(s) 962
Eam1104I CTCTTC 1 cut(s) 1352
EarI CTCTTC 1 cut(s) 1352
EciI GGCGGA 1 cut(s) 512
EclXI CGGCCG 1 cut(s) 962
Eco130I CCWWGG 3 cut(s) 754, 851, 861
Eco147I AGGCCT 1 cut(s) 838
Eco31I GGTCTC 1 cut(s) 1411
Eco47I GGWCC 1 cut(s) 778
Eco52I CGGCCG 1 cut(s) 962
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 3 cut(s) 162, 492, 549
EcoT14I CCWWGG 3 cut(s) 754, 851, 861
EcoT22I ATGCAT 2 cut(s) 95, 532
ErhI CCWWGG 3 cut(s) 754, 851, 861
FaqI GGGAC 2 cut(s) 827, 1429
FblI GTMKAC 1 cut(s) 1332
Fnu4HI GCNGC 4 cut(s) 7, 489, 760, 1140
FokI GGATG 4 cut(s) 246, 257, 1100, 1426
Fsp4HI GCNGC 4 cut(s) 7, 489, 760, 1140
FspBI CTAG 4 cut(s) 423, 1032, 1175, 1221
FspI TGCGCA 1 cut(s) 1186
GlaI GCGC 2 cut(s) 525, 1186
GluI GCNGC 4 cut(s) 7, 489, 760, 1140
HapII CCGG 3 cut(s) 450, 460, 465
HgaI GACGC 3 cut(s) 618, 865, 1139
HhaI GCGC 2 cut(s) 526, 1187
Hin1I GRCGYC 1 cut(s) 857
Hin6I GCGC 2 cut(s) 524, 1185
HinP1I GCGC 2 cut(s) 524, 1185
HincII GTYRAC 1 cut(s) 695
HindII GTYRAC 1 cut(s) 695
HinfI GANTC 2 cut(s) 886, 1349
HpaII CCGG 3 cut(s) 450, 460, 465
HphI GGTGA 5 cut(s) 190, 631, 743, 952, 1385
Hpy166II GTNNAC 7 cut(s) 145, 695, 778, 990, 1098, 1291, 1333
Hpy188I TCNGA 6 cut(s) 226, 478, 574, 891, 1213, 1482
Hpy188III TCNNGA 4 cut(s) 41, 1058, 1399, 1510
Hpy8I GTNNAC 7 cut(s) 145, 695, 778, 990, 1098, 1291, 1333
Hpy99I CGWCG 3 cut(s) 602, 612, 1155
HpyAV CCTTC 6 cut(s) 98, 146, 673, 1037, 1105, 1306
HpyCH4III ACNGT 5 cut(s) 124, 643, 782, 898, 985
HpyCH4IV ACGT 1 cut(s) 1302
HpyF10VI GCNNNNNNNGC 7 cut(s) 494, 551, 560, 659, 856, 1265, 1292
HpyF3I CTNAG 3 cut(s) 665, 1132, 1479
HpySE526I ACGT 1 cut(s) 1302
Hsp92I GRCGYC 1 cut(s) 857
HspAI GCGC 2 cut(s) 524, 1185
Kzo9I GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 4 cut(s) 18, 475, 771, 1126
LweI GCATC 3 cut(s) 102, 217, 1078
MaeI CTAG 4 cut(s) 423, 1032, 1175, 1221
MaeII ACGT 1 cut(s) 1302
MaeIII GTNAC 3 cut(s) 212, 280, 940
MalI GATC 6 cut(s) 39, 482, 501, 960, 1346, 1514
MboI GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
MboII GAAGA 7 cut(s) 25, 125, 213, 638, 718, 904, 1369
MfeI CAATTG 1 cut(s) 688
MflI RGATCY 2 cut(s) 480, 499
MhlI GDGCHC 3 cut(s) 432, 992, 1039
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 4 cut(s) 688, 1014, 1364, 1456
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 2 cut(s) 895, 1358
MmeI TCCRAC 2 cut(s) 849, 869
Mox20I TGGCCA 1 cut(s) 350
Mph1103I ATGCAT 2 cut(s) 95, 532
MroXI GAANNNNTTC 1 cut(s) 156
MscI TGGCCA 1 cut(s) 350
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 5 cut(s) 411, 443, 1001, 1080, 1412
Msp20I TGGCCA 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 3 cut(s) 450, 460, 465
MspR9I CCNGG 4 cut(s) 164, 465, 494, 551
MunI CAATTG 1 cut(s) 688
MvaI CCWGG 3 cut(s) 164, 494, 551
MwoI GCNNNNNNNGC 7 cut(s) 494, 551, 560, 659, 856, 1265, 1292
NciI CCSGG 1 cut(s) 465
NdeII GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
NlaIV GGNNCC 3 cut(s) 337, 536, 684
NmeAIII GCCGAG 1 cut(s) 990
NmuCI GTSAC 1 cut(s) 940
NsbI TGCGCA 1 cut(s) 1186
NsiI ATGCAT 2 cut(s) 95, 532
NspI RCATGY 4 cut(s) 530, 558, 1006, 1471
NspV TTCGAA 1 cut(s) 384
OliI CACNNNNGTG 1 cut(s) 411
PaeI GCATGC 1 cut(s) 530
PceI AGGCCT 1 cut(s) 838
PcsI WCGNNNNNNNCGW 1 cut(s) 1299
PdmI GAANNNNTTC 1 cut(s) 156
PfoI TCCNGGA 1 cut(s) 162
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 4 cut(s) 8, 490, 761, 1141
PleI GAGTC 2 cut(s) 894, 1357
PpsI GAGTC 2 cut(s) 894, 1357
Psp6I CCWGG 3 cut(s) 162, 492, 549
PspGI CCWGG 3 cut(s) 162, 492, 549
PspN4I GGNNCC 3 cut(s) 337, 536, 684
PspPI GGNCC 5 cut(s) 74, 335, 462, 778, 824
PsuI RGATCY 2 cut(s) 480, 499
RsaI GTAC 5 cut(s) 325, 454, 769, 1099, 1499
RsaNI GTAC 5 cut(s) 324, 453, 768, 1098, 1498
RseI CAYNNNNRTG 5 cut(s) 411, 443, 1001, 1080, 1412
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 4 cut(s) 7, 489, 760, 1140
Sau3AI GATC 6 cut(s) 37, 480, 499, 958, 1344, 1512
Sau96I GGNCC 5 cut(s) 74, 335, 462, 778, 824
ScaI AGTACT 1 cut(s) 769
SchI GAGTC 2 cut(s) 895, 1358
ScrFI CCNGG 4 cut(s) 164, 465, 494, 551
SduI GDGCHC 3 cut(s) 432, 992, 1039
SfaNI GCATC 3 cut(s) 102, 217, 1078
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 1 cut(s) 778
SmiMI CAYNNNNRTG 5 cut(s) 411, 443, 1001, 1080, 1412
SmlI CTYRAG 1 cut(s) 832
SmoI CTYRAG 1 cut(s) 832
SphI GCATGC 1 cut(s) 530
Sse9I AATT 4 cut(s) 688, 1014, 1364, 1456
SseBI AGGCCT 1 cut(s) 838
SsiI CCGC 3 cut(s) 486, 497, 1247
SspI AATATT 1 cut(s) 748
SspMI CTAG 4 cut(s) 423, 1032, 1175, 1221
StuI AGGCCT 1 cut(s) 838
StyD4I CCNGG 4 cut(s) 162, 463, 492, 549
StyI CCWWGG 3 cut(s) 754, 851, 861
TaaI ACNGT 5 cut(s) 124, 643, 782, 898, 985
TaiI ACGT 1 cut(s) 1305
TaqI TCGA 9 cut(s) 96, 384, 597, 948, 1072, 1080, 1255, 1347, 1352
TaqII GACCGA 1 cut(s) 1435
TasI AATT 4 cut(s) 688, 1014, 1364, 1456
TatI WGTACW 2 cut(s) 767, 1097
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 3 cut(s) 418, 903, 1003
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 4 cut(s) 6, 488, 759, 1139
Tsp45I GTSAC 1 cut(s) 940
TspDTI ATGAA 6 cut(s) 26, 206, 229, 242, 279, 1370
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 3 cut(s) 418, 903, 1003
VneI GTGCAC 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 778
XceI RCATGY 4 cut(s) 530, 558, 1006, 1471
XmiI GTMKAC 1 cut(s) 1332
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 4 cut(s) 423, 1032, 1175, 1221
ZrmI AGTACT 1 cut(s) 769
Zsp2I ATGCAT 2 cut(s) 95, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.