Rw6G033540

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011257
Physical Location & Seq
Reverse (-)
58088078 .. 58090388
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033540.1

Sequence Viewer

Length: 1416 bp
ATGAAGAAAGTTTGTTGGCCTTACTTTGATCCTGACTTTCATAATCTCCCAGAAAGGATCTATGGCCCTGTATGTAGAGTATCCCTCGACAACGAGACTTTGGAAGATTGCACATTAGTAAAGGTAGATAGCGTGAACAAGCAAGGGCTTCTCCTCGAAGTGATTCAAGTATTGACAGACATGAATCTTACCATCACTAAATGTTTTATATCTTCCGATGCAGAATGTTTTCATGTTAAAGATGAGAATGGCAACAAACTTACAGACCAGAATGCCATTAACTATATCCAGCAGGCCATAGGTACAACTACAGGTCCTCCAGACTCCACCAGGGCCAATGCCTATGCCAGCAATATTTTCGAATCCGAAAACCATAGCGAGCATACGACCATCGAAATGACGGGAACGGACCGACCTGGTCTCTTCTCGGAGATATCGGCTGCCCTTGCCGACCTCCAATGCAACATAGTTGAAGCCCATGCGTGGACCCATAACGGCCGGCTAGCTTGTGTTGCTCAAATATCTGACCAATCCACTGACGCCCCAATCGAGGACCCCCACCGCCTTGCCACCATCGAAGACCACCTCATCACCGTCCTTCGAGCCACCACTTCCGTAAGCCCTTGTGGGAAAGAAGCAAACAACCAAGAAGTGAAGACCACAGGGTTTTTAGGAGGAGACTATCAAGGCACCATGACTAATGTCGAAAGGAGGTTACACCAACTAATGCTTTCCATTAGGGACTTTGACTGGCCCCAATTTGAGGCCCGCCAGCTCTCCGAGGACGCCATTCACGGCGGTTTGGATCGAAGCTGCAAGGAGAAAGGGTATTCCATGGTGAGCTTAGAGTGTAAGGATCGGCCGAGGCTTATGTTTGATACTGTGTGCACTCTAACTGACATGCAGTATGTGATTTTCCATGCTTCCACTCATTGCCATGAAGGATCTGCCTTTCAGGAATACTTTATCCGGCATGTTGACGGGGATGTCTTGTGCACAGAGAGCGAGAAGGAACGAGTTATAAAATGCTTAGAGGCTGCTATTGAGCGTCGAGTTTGTGAGGGGATTCGACTAGAGCTGCGTGCCGACGATAGGATGGGATTGCTATCGGATATAACTAGGGTTCTACGAGAGAATGGACTTGCTGTTGTTCGAGCAGATGTAGCAACCCAAGGAGAGAAGTCGATTAACGCTTTCTATGTGAGGGACATTTCAGGGAACGAAGTAGTTCCAGACATGGATTTTGTTGAGTCCATGAAGAGAGAATTGATGGGTCCAAGTCATATAAACCTTCAAGTCAAGAATGAGAAACATCTCAGATCACCAAGCTCAACTGAAAAGTCTCGTTATTTCTCAAATATTGGAGATATTCTAAAATCTCAAATTGGGCGTTTCTCTTTTGCTAAGATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT ACT_6

Protein Analysis

471

Amino Acids

52.99

Weight (kDa)

5.28

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 129 - 166 1.7e-06 ACT domain
ACT_6 PF13740 131 - 175 3.2e-07 ACT domain
ACT PF01842 357 - 407 3e-06 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25320 AT5G25320 AT5G25320
fragaria_vesca FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840 FvH4_2g28840
malus_domestica MD08G1156600.v1.1 MD15G1127200.v1.1
prunus_persica Prupe.1G482900_v2.0.a1
pyrus_communis pycom08g12880
rosa_chinensis RchiOBHm_Chr6g0297601
rosa_laevigata RLG00000011578
rosa_rugosa Rorug06G0274200
rosa_samantha Rh6AG385000 Rh6BG393100 Rh6CG398900 Rh6DG385500
rosa_wichuraiana Rw6G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1022
AasI GACNNNNNNGTC 1 cut(s) 986
AccB1I GGYRCC 1 cut(s) 689
AciI CCGC 3 cut(s) 562, 769, 798
AclWI GGATC 5 cut(s) 23, 65, 813, 864, 952
AcoI YGGCCR 2 cut(s) 496, 860
AcyI GRCGYC 2 cut(s) 540, 786
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 4 cut(s) 483, 550, 763, 1092
AgsI TTSAA 3 cut(s) 167, 473, 1295
AjnI CCWGG 2 cut(s) 329, 415
AluBI AGCT 6 cut(s) 506, 775, 813, 843, 1078, 1329
AluI AGCT 6 cut(s) 506, 775, 813, 843, 1078, 1329
Alw21I GWGCWC 2 cut(s) 890, 998
Alw26I GTCTC 4 cut(s) 89, 425, 672, 1347
Alw44I GTGCAC 2 cut(s) 886, 994
AlwI GGATC 5 cut(s) 23, 65, 813, 864, 952
AoxI GGCC 8 cut(s) 17, 64, 294, 333, 496, 752, 765, 860
ApaLI GTGCAC 2 cut(s) 886, 994
ApeKI GCWGC 4 cut(s) 440, 813, 1037, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 1226, 1258
Asp700I GAANNNNTTC 3 cut(s) 162, 228, 1227
AspS9I GGNCC 9 cut(s) 65, 314, 333, 409, 486, 553, 753, 766, 1274
AsuHPI GGTGA 3 cut(s) 583, 850, 1314
AsuII TTCGAA 1 cut(s) 360
AsuNHI GCTAGC 1 cut(s) 502
AvaII GGWCC 5 cut(s) 314, 409, 486, 553, 1274
BaeGI GKGCMC 2 cut(s) 890, 998
BanI GGYRCC 1 cut(s) 689
BbsI GAAGAC 2 cut(s) 585, 662
Bbv12I GWGCWC 2 cut(s) 890, 998
BbvI GCAGC 4 cut(s) 427, 800, 1024, 1065
BccI CCATC 5 cut(s) 200, 398, 581, 1090, 1264
BceAI ACGGC 2 cut(s) 511, 811
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BciT130I CCWGG 2 cut(s) 331, 417
BciVI GTATCC 1 cut(s) 91
BcoDI GTCTC 4 cut(s) 89, 425, 672, 1347
BfaI CTAG 3 cut(s) 503, 1073, 1119
BfmI CTRYAG 1 cut(s) 309
BfuI GTATCC 1 cut(s) 91
BisI GCNGC 4 cut(s) 441, 814, 1038, 1079
BlsI GCNGC 4 cut(s) 442, 815, 1039, 1080
Bme1390I CCNGG 2 cut(s) 331, 417
Bme18I GGWCC 5 cut(s) 314, 409, 486, 553, 1274
BmgT120I GGNCC 9 cut(s) 65, 314, 333, 409, 486, 553, 753, 766, 1274
BmiI GGNNCC 5 cut(s) 488, 555, 691, 755, 1275
BmrFI CCNGG 2 cut(s) 331, 417
BmsI GCATC 1 cut(s) 208
BmtI GCTAGC 1 cut(s) 506
BoxI GACNNNNGTC 1 cut(s) 701
BpiI GAAGAC 2 cut(s) 585, 662
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 360
BsaBI GATNNNNATC 1 cut(s) 1105
BsaHI GRCGYC 2 cut(s) 540, 786
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 5 cut(s) 330, 780, 834, 863, 1171
BsaXI ACNNNNNCTCC 4 cut(s) 301, 331, 812, 842
Bsc4I CCNNNNNNNGG 4 cut(s) 483, 550, 763, 1092
Bse118I RCCGGY 1 cut(s) 498
Bse1I ACTGG 1 cut(s) 755
Bse3DI GCAATG 1 cut(s) 931
Bse8I GATNNNNATC 1 cut(s) 1105
BseBI CCWGG 2 cut(s) 331, 417
BseDI CCNNGG 5 cut(s) 330, 780, 834, 863, 1171
BseGI GGATG 2 cut(s) 991, 1101
BseJI GATNNNNATC 1 cut(s) 1105
BseLI CCNNNNNNNGG 4 cut(s) 483, 550, 763, 1092
BseMI GCAATG 1 cut(s) 931
BseMII CTCAG 1 cut(s) 1330
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 2 cut(s) 143, 690
BseSI GKGCMC 2 cut(s) 890, 998
BseX3I CGGCCG 2 cut(s) 496, 860
BseXI GCAGC 4 cut(s) 427, 800, 1024, 1065
Bsh1285I CGRYCG 2 cut(s) 499, 863
BshFI GGCC 8 cut(s) 19, 66, 296, 335, 498, 754, 767, 862
BshNI GGYRCC 1 cut(s) 689
BsiEI CGRYCG 2 cut(s) 499, 863
BsiHKAI GWGCWC 2 cut(s) 890, 998
BsiSI CCGG 2 cut(s) 499, 970
BslFI GGGAC 2 cut(s) 755, 1220
BslI CCNNNNNNNGG 4 cut(s) 483, 550, 763, 1092
BsmAI GTCTC 4 cut(s) 89, 425, 672, 1347
BsmFI GGGAC 2 cut(s) 755, 1220
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 8 cut(s) 19, 66, 296, 335, 498, 754, 767, 862
Bso31I GGTCTC 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 360
Bsp1286I GDGCHC 2 cut(s) 890, 998
Bsp143I GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 3 cut(s) 562, 769, 798
BspANI GGCC 8 cut(s) 19, 66, 296, 335, 498, 754, 767, 862
BspCNI CTCAG 1 cut(s) 1329
BspLI GGNNCC 5 cut(s) 488, 555, 691, 755, 1275
BspOI GCTAGC 1 cut(s) 506
BspPI GGATC 5 cut(s) 23, 65, 813, 864, 952
BspT104I TTCGAA 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 931
BsrFI RCCGGY 1 cut(s) 498
BsrI ACTGG 1 cut(s) 755
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 5 cut(s) 330, 780, 834, 863, 1171
BssMI GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
BssNI GRCGYC 2 cut(s) 540, 786
BssT1I CCWWGG 2 cut(s) 834, 1171
Bst2UI CCWGG 2 cut(s) 331, 417
Bst4CI ACNGT 2 cut(s) 595, 883
Bst6I CTCTTC 2 cut(s) 428, 1253
BstACI GRCGYC 2 cut(s) 540, 786
BstBI TTCGAA 1 cut(s) 360
BstC8I GCNNGC 8 cut(s) 294, 349, 380, 500, 504, 769, 773, 1083
BstDEI CTNAG 4 cut(s) 844, 1030, 1316, 1404
BstDSI CCRYGG 1 cut(s) 834
BstF5I GGATG 2 cut(s) 991, 1101
BstKTI GATC 7 cut(s) 31, 60, 808, 859, 947, 1322, 1410
BstMAI GTCTC 4 cut(s) 89, 425, 672, 1347
BstMBI GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
BstMCI CGRYCG 2 cut(s) 499, 863
BstMWI GCNNNNNNNGC 4 cut(s) 446, 512, 1002, 1163
BstNI CCWGG 2 cut(s) 331, 417
BstNSI RCATGY 2 cut(s) 904, 977
BstPAI GACNNNNGTC 1 cut(s) 701
BstSCI CCNGG 2 cut(s) 329, 415
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 2 cut(s) 890, 998
BstV1I GCAGC 4 cut(s) 427, 800, 1024, 1065
BstV2I GAAGAC 2 cut(s) 585, 662
BstX2I RGATCY 2 cut(s) 57, 944
BstYI RGATCY 2 cut(s) 57, 944
BstZI CGGCCG 2 cut(s) 496, 860
BsuI GTATCC 1 cut(s) 91
BsuRI GGCC 8 cut(s) 19, 66, 296, 335, 498, 754, 767, 862
BtgI CCRYGG 1 cut(s) 834
BtsCI GGATG 2 cut(s) 991, 1101
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 8 cut(s) 294, 349, 380, 500, 504, 769, 773, 1083
Cfr10I RCCGGY 1 cut(s) 498
Cfr13I GGNCC 9 cut(s) 65, 314, 333, 409, 486, 553, 753, 766, 1274
CpoI CGGWCCG 1 cut(s) 409
CseI GACGC 3 cut(s) 548, 794, 1037
CsiI ACCWGGT 1 cut(s) 415
Csp6I GTAC 1 cut(s) 303
CspI CGGWCCG 1 cut(s) 409
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 4 cut(s) 844, 1030, 1316, 1404
DpnI GATC 7 cut(s) 30, 59, 807, 858, 946, 1321, 1409
DpnII GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
DrdI GACNNNNNNGTC 1 cut(s) 986
DseDI GACNNNNNNGTC 1 cut(s) 986
EaeI YGGCCR 2 cut(s) 496, 860
EagI CGGCCG 2 cut(s) 496, 860
Eam1104I CTCTTC 2 cut(s) 428, 1253
EarI CTCTTC 2 cut(s) 428, 1253
EclXI CGGCCG 2 cut(s) 496, 860
Eco130I CCWWGG 2 cut(s) 834, 1171
Eco31I GGTCTC 1 cut(s) 425
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 5 cut(s) 314, 409, 486, 553, 1274
Eco52I CGGCCG 2 cut(s) 496, 860
EcoO109I RGGNCCY 2 cut(s) 314, 553
EcoRII CCWGG 2 cut(s) 329, 415
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 834, 1171
ErhI CCWWGG 2 cut(s) 834, 1171
FaqI GGGAC 2 cut(s) 755, 1220
FauI CCCGC 1 cut(s) 776
Fnu4HI GCNGC 4 cut(s) 441, 814, 1038, 1079
FokI GGATG 2 cut(s) 998, 1108
Fsp4HI GCNGC 4 cut(s) 441, 814, 1038, 1079
FspBI CTAG 3 cut(s) 503, 1073, 1119
GluI GCNGC 4 cut(s) 441, 814, 1038, 1079
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 8 cut(s) 19, 66, 296, 335, 498, 754, 767, 862
HapII CCGG 2 cut(s) 499, 970
HgaI GACGC 3 cut(s) 548, 794, 1037
Hin1I GRCGYC 2 cut(s) 540, 786
HincII GTYRAC 1 cut(s) 979
HindII GTYRAC 1 cut(s) 979
HinfI GANTC 6 cut(s) 163, 184, 323, 362, 1066, 1250
HpaII CCGG 2 cut(s) 499, 970
HphI GGTGA 3 cut(s) 583, 850, 1314
Hpy166II GTNNAC 5 cut(s) 136, 486, 888, 979, 996
Hpy188I TCNGA 7 cut(s) 217, 367, 430, 526, 781, 1111, 1319
Hpy188III TCNNGA 5 cut(s) 32, 320, 956, 1232, 1300
Hpy8I GTNNAC 5 cut(s) 136, 486, 888, 979, 996
Hpy99I CGWCG 2 cut(s) 1053, 1091
HpyAV CCTTC 4 cut(s) 608, 935, 1003, 1301
HpyCH4III ACNGT 2 cut(s) 595, 883
HpyCH4V TGCA 7 cut(s) 111, 221, 462, 816, 888, 904, 996
HpyF10VI GCNNNNNNNGC 4 cut(s) 446, 512, 1002, 1163
HpyF3I CTNAG 4 cut(s) 844, 1030, 1316, 1404
Hsp92I GRCGYC 2 cut(s) 540, 786
KroI GCCGGC 1 cut(s) 498
KroNI GCCGGC 1 cut(s) 500
Kzo9I GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
Lsp1109I GCAGC 4 cut(s) 427, 800, 1024, 1065
LweI GCATC 1 cut(s) 208
MabI ACCWGGT 1 cut(s) 415
MaeI CTAG 3 cut(s) 503, 1073, 1119
MaeIII GTNAC 1 cut(s) 714
MalI GATC 7 cut(s) 30, 59, 807, 858, 946, 1321, 1409
MboI GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
MboII GAAGA 7 cut(s) 16, 116, 204, 415, 590, 667, 1270
MflI RGATCY 2 cut(s) 57, 944
MhlI GDGCHC 2 cut(s) 890, 998
MluCI AATT 3 cut(s) 758, 1265, 1383
MlyI GAGTC 2 cut(s) 317, 1259
MroNI GCCGGC 1 cut(s) 498
MroXI GAANNNNTTC 3 cut(s) 162, 228, 1227
MseI TTAA 3 cut(s) 237, 279, 1188
MslI CAYNNNNRTG 2 cut(s) 395, 936
MspI CCGG 2 cut(s) 499, 970
MspR9I CCNGG 2 cut(s) 331, 417
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 2 cut(s) 331, 417
MwoI GCNNNNNNNGC 4 cut(s) 446, 512, 1002, 1163
NaeI GCCGGC 1 cut(s) 500
NcoI CCATGG 1 cut(s) 834
NdeII GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
NgoMIV GCCGGC 1 cut(s) 498
NheI GCTAGC 1 cut(s) 502
NlaIV GGNNCC 5 cut(s) 488, 555, 691, 755, 1275
NmeAIII GCCGAG 1 cut(s) 888
NspI RCATGY 2 cut(s) 904, 977
NspV TTCGAA 1 cut(s) 360
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PctI GAATGC 1 cut(s) 277
PdiI GCCGGC 1 cut(s) 500
PdmI GAANNNNTTC 3 cut(s) 162, 228, 1227
PfeI GAWTC 4 cut(s) 163, 184, 362, 1066
PflFI GACNNNGTC 1 cut(s) 417
PkrI GCNGC 4 cut(s) 442, 815, 1039, 1080
PleI GAGTC 2 cut(s) 317, 1258
PpsI GAGTC 2 cut(s) 317, 1258
PpuMI RGGWCCY 2 cut(s) 314, 553
PshAI GACNNNNGTC 1 cut(s) 701
PsiI TTATAA 1 cut(s) 1022
Psp5II RGGWCCY 2 cut(s) 314, 553
Psp6I CCWGG 2 cut(s) 329, 415
PspGI CCWGG 2 cut(s) 329, 415
PspN4I GGNNCC 5 cut(s) 488, 555, 691, 755, 1275
PspPI GGNCC 9 cut(s) 65, 314, 333, 409, 486, 553, 753, 766, 1274
PspPPI RGGWCCY 2 cut(s) 314, 553
PsuI RGATCY 2 cut(s) 57, 944
PsyI GACNNNGTC 1 cut(s) 417
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
RseI CAYNNNNRTG 2 cut(s) 395, 936
Rsr2I CGGWCCG 1 cut(s) 409
RsrII CGGWCCG 1 cut(s) 409
SaqAI TTAA 3 cut(s) 237, 279, 1188
SatI GCNGC 4 cut(s) 441, 814, 1038, 1079
Sau3AI GATC 7 cut(s) 28, 57, 805, 856, 944, 1319, 1407
Sau96I GGNCC 9 cut(s) 65, 314, 333, 409, 486, 553, 753, 766, 1274
SchI GAGTC 2 cut(s) 317, 1259
ScrFI CCNGG 2 cut(s) 331, 417
SduI GDGCHC 2 cut(s) 890, 998
SexAI ACCWGGT 1 cut(s) 415
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 360
SinI GGWCC 5 cut(s) 314, 409, 486, 553, 1274
SmiMI CAYNNNNRTG 2 cut(s) 395, 936
Sse9I AATT 3 cut(s) 758, 1265, 1383
SsiI CCGC 3 cut(s) 562, 769, 798
SspI AATATT 2 cut(s) 355, 1360
SspMI CTAG 3 cut(s) 503, 1073, 1119
StyD4I CCNGG 2 cut(s) 329, 415
StyI CCWWGG 2 cut(s) 834, 1171
TaaI ACNGT 2 cut(s) 595, 883
TaqII GACCGA 1 cut(s) 426
TasI AATT 3 cut(s) 758, 1265, 1383
TfiI GAWTC 4 cut(s) 163, 184, 362, 1066
Tru1I TTAA 3 cut(s) 237, 279, 1188
Tru9I TTAA 3 cut(s) 237, 279, 1188
TscAI CASTG 1 cut(s) 541
TseI GCWGC 4 cut(s) 440, 813, 1037, 1078
TspDTI ATGAA 6 cut(s) 17, 29, 197, 221, 954, 1271
TspGWI ACGGA 2 cut(s) 422, 604
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 417
VneI GTGCAC 2 cut(s) 886, 994
VpaK11BI GGWCC 5 cut(s) 314, 409, 486, 553, 1274
XceI RCATGY 2 cut(s) 904, 977
XmnI GAANNNNTTC 3 cut(s) 162, 228, 1227
XspI CTAG 3 cut(s) 503, 1073, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.