AT5G25390

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010712
Physical Location & Seq
Forward (+)
8820458 .. 8822062
1605 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25390.2

Sequence Viewer

Length: 561 bp
ATGGTACATTCGAAGAAGTTCCGAGGTGTCCGCCAGCGTCAGTGGGGTTCTTGGGTTTCTGAGATTCGTCATCCTCTCTTAGTGTGGCTAGGAACATTCGACACGGCGGAAACAGCGGCTAGAGCCTACGACCAAGCCGCGGTTCTAATGAACGGCCAGAGCGCGAAGACTAACTTCCCCGTCATCAAATCGAACGGTTCAAATTCCTTGGAGATTAACTCTGCGTTAAGGTCTCCCAAATCATTATCGGAACTATTGAACGCTAAGCTAAGGAAGAACTGTAAAGACCAGACACCGTATCTGACGTGTCTCCGCCTCGACAACGACAGCTCACACATCGGCGTCTGGCAGAAACGCGCCGGGTCAAAAACGAGTCCAAACTGGGTCAAGCTTGTTGAACTAGGTGACAAAGTTAACGCACGTCCCGGTGGTGATATTGAGACTAATAAGATGAAGGTACGAAACGAAGACGTTCAGGAAGATGATCAAATGGCGATGCAGATGATCGAGGAGTTGCTTAACTGGACCTGTCCTGGATCTGGATCCATTGCACAGGTCTAA

Protein Analysis

186

Amino Acids

20.81

Weight (kDa)

9.19

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 6 - 54 6.6e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010712)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11190 AT5G25390 AT5G25390
fragaria_vesca FvH4_2g29150
malus_domestica MD08G1149200.v1.1 MD15G1124900.v1.1
prunus_persica Prupe.1G480400_v2.0.a1
pyrus_communis pycom08g12560 pycom15g11250
rosa_chinensis RchiOBHm_Chr6g0298011
rosa_laevigata RLG00000011543
rosa_multiflora Rmu_sc0002800.1_g000022
rosa_roxburghii Rroxscaffold_7G00170000 Rroxscaffold_7G00178830
rosa_rugosa Rorug06G0278000
rosa_samantha Rh6AG388300 Rh6BG396500 Rh6CG402500 Rh6DG388700
rosa_wichuraiana Rw6G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 140, 164, 357
AciI CCGC 6 cut(s) 31, 107, 116, 138, 140, 313
AclWI GGATC 3 cut(s) 537, 544, 550
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 2 cut(s) 6, 459
AfiI CCNNNNNNNGG 2 cut(s) 139, 539
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 3 cut(s) 201, 259, 398
AjiI CACGTC 2 cut(s) 306, 422
AjnI CCWGG 1 cut(s) 532
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 3 cut(s) 268, 330, 391
AluI AGCT 3 cut(s) 268, 330, 391
Alw26I GTCTC 3 cut(s) 237, 314, 434
AlwI GGATC 3 cut(s) 537, 544, 550
AoxI GGCC 1 cut(s) 154
ApoI RAATTY 1 cut(s) 202
Asp700I GAANNNNTTC 2 cut(s) 17, 471
AspLEI GCGC 2 cut(s) 164, 359
AspS9I GGNCC 1 cut(s) 525
AsuC2I CCSGG 2 cut(s) 361, 426
AsuHPI GGTGA 2 cut(s) 416, 443
AsuII TTCGAA 1 cut(s) 11
AvaII GGWCC 1 cut(s) 525
BamHI GGATCC 1 cut(s) 542
BbsI GAAGAC 2 cut(s) 173, 474
BceAI ACGGC 2 cut(s) 120, 169
BciT130I CCWGG 1 cut(s) 534
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 2 cut(s) 361, 426
BcoDI GTCTC 3 cut(s) 237, 314, 434
BfaI CTAG 3 cut(s) 89, 120, 401
BisI GCNGC 2 cut(s) 117, 138
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 2 cut(s) 118, 139
Bme1390I CCNGG 3 cut(s) 361, 426, 534
Bme18I GGWCC 1 cut(s) 525
BmgBI CACGTC 2 cut(s) 306, 422
BmgT120I GGNCC 1 cut(s) 525
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 3 cut(s) 361, 426, 534
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 1 cut(s) 486
BmuI ACTGGG 1 cut(s) 391
BpiI GAAGAC 2 cut(s) 173, 474
BplI GAGNNNNNCTC 2 cut(s) 203, 235
Bpu10I CCTNAGC 1 cut(s) 269
Bpu1102I GCTNAGC 1 cut(s) 264
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 2 cut(s) 361, 426
BsaBI GATNNNNATC 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 3 cut(s) 22, 138, 207
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 539
Bse1I ACTGG 2 cut(s) 386, 527
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 1 cut(s) 541
BseBI CCWGG 1 cut(s) 534
BseDI CCNNGG 3 cut(s) 22, 138, 207
BseGI GGATG 1 cut(s) 70
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 2 cut(s) 139, 539
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 2 cut(s) 386, 527
BseRI GAGGAG 1 cut(s) 524
Bsh1236I CGCG 3 cut(s) 140, 164, 357
BshFI GGCC 1 cut(s) 156
BsiSI CCGG 2 cut(s) 360, 426
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 2 cut(s) 139, 539
BsmAI GTCTC 3 cut(s) 237, 314, 434
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 1 cut(s) 156
Bso31I GGTCTC 1 cut(s) 237
Bsp119I TTCGAA 1 cut(s) 11
Bsp143I GATC 4 cut(s) 484, 504, 536, 542
Bsp1720I GCTNAGC 1 cut(s) 264
BspACI CCGC 6 cut(s) 31, 107, 116, 138, 140, 313
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 52
BspFNI CGCG 3 cut(s) 140, 164, 357
BspLI GGNNCC 1 cut(s) 544
BspPI GGATC 3 cut(s) 537, 544, 550
BspT104I TTCGAA 1 cut(s) 11
BspTNI GGTCTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 2 cut(s) 386, 527
BssECI CCNNGG 3 cut(s) 22, 138, 207
BssMI GATC 4 cut(s) 484, 504, 536, 542
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 3 cut(s) 197, 281, 297
BstACI GRCGYC 1 cut(s) 342
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 4 cut(s) 60, 79, 264, 269
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 70
BstFNI CGCG 3 cut(s) 140, 164, 357
BstHHI GCGC 2 cut(s) 164, 359
BstKTI GATC 4 cut(s) 487, 507, 539, 545
BstMAI GTCTC 3 cut(s) 237, 314, 434
BstMBI GATC 4 cut(s) 484, 504, 536, 542
BstMWI GCNNNNNNNGC 2 cut(s) 113, 122
BstNI CCWGG 1 cut(s) 534
BstSCI CCNGG 3 cut(s) 359, 424, 532
BstUI CGCG 3 cut(s) 140, 164, 357
BstV2I GAAGAC 2 cut(s) 173, 474
BstX2I RGATCY 2 cut(s) 536, 542
BstYI RGATCY 2 cut(s) 536, 542
BsuRI GGCC 1 cut(s) 156
BtgI CCRYGG 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 509
BtrI CACGTC 2 cut(s) 306, 422
BtsCI GGATG 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 35
CfoI GCGC 2 cut(s) 164, 359
Cfr13I GGNCC 1 cut(s) 525
Cfr42I CCGCGG 1 cut(s) 141
CseI GACGC 2 cut(s) 26, 331
Csp6I GTAC 2 cut(s) 5, 458
CviJI RGCY 8 cut(s) 88, 119, 125, 137, 156, 268, 330, 391
CviKI_1 RGCY 8 cut(s) 88, 119, 125, 137, 156, 268, 330, 391
CviQI GTAC 2 cut(s) 5, 458
DdeI CTNAG 4 cut(s) 60, 79, 264, 269
DpnI GATC 4 cut(s) 486, 506, 538, 544
DpnII GATC 4 cut(s) 484, 504, 536, 542
EaeI YGGCCR 1 cut(s) 154
EciI GGCGGA 3 cut(s) 20, 122, 302
Eco130I CCWWGG 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FaqI GGGAC 1 cut(s) 408
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 2 cut(s) 117, 138
FokI GGATG 1 cut(s) 57
Fsp4HI GCNGC 2 cut(s) 117, 138
FspBI CTAG 3 cut(s) 89, 120, 401
GlaI GCGC 2 cut(s) 163, 358
GluI GCNGC 2 cut(s) 117, 138
HaeIII GGCC 1 cut(s) 156
HapII CCGG 2 cut(s) 360, 426
HgaI GACGC 2 cut(s) 26, 331
HhaI GCGC 2 cut(s) 164, 359
Hin1I GRCGYC 1 cut(s) 342
Hin6I GCGC 2 cut(s) 162, 357
HinP1I GCGC 2 cut(s) 162, 357
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 2 cut(s) 64, 373
HpaI GTTAAC 1 cut(s) 415
HpaII CCGG 2 cut(s) 360, 426
HphI GGTGA 2 cut(s) 416, 443
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 4 cut(s) 23, 61, 250, 303
Hpy188III TCNNGA 2 cut(s) 476, 540
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 1 cut(s) 448
HpyCH4III ACNGT 3 cut(s) 197, 281, 297
HpyCH4IV ACGT 3 cut(s) 305, 421, 471
HpyCH4V TGCA 2 cut(s) 499, 551
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 122
HpyF3I CTNAG 4 cut(s) 60, 79, 264, 269
HpySE526I ACGT 3 cut(s) 305, 421, 471
Hsp92I GRCGYC 1 cut(s) 342
HspAI GCGC 2 cut(s) 162, 357
Ksp22I TGATCA 1 cut(s) 484
KspAI GTTAAC 1 cut(s) 415
KspI CCGCGG 1 cut(s) 141
Kzo9I GATC 4 cut(s) 484, 504, 536, 542
LweI GCATC 1 cut(s) 486
MaeI CTAG 3 cut(s) 89, 120, 401
MaeII ACGT 3 cut(s) 305, 421, 471
MaeIII GTNAC 1 cut(s) 404
MalI GATC 4 cut(s) 486, 506, 538, 544
MboI GATC 4 cut(s) 484, 504, 536, 542
MboII GAAGA 5 cut(s) 25, 178, 286, 479, 491
MflI RGATCY 2 cut(s) 536, 542
MluCI AATT 1 cut(s) 202
MlyI GAGTC 1 cut(s) 382
MnlI CCTC 4 cut(s) 17, 84, 326, 502
MroXI GAANNNNTTC 2 cut(s) 17, 471
MseI TTAA 4 cut(s) 216, 227, 414, 519
MspA1I CMGCKG 2 cut(s) 116, 140
MspI CCGG 2 cut(s) 360, 426
MspR9I CCNGG 3 cut(s) 361, 426, 534
MvaI CCWGG 1 cut(s) 534
MvnI CGCG 3 cut(s) 140, 164, 357
MwoI GCNNNNNNNGC 2 cut(s) 113, 122
NciI CCSGG 2 cut(s) 361, 426
NdeII GATC 4 cut(s) 484, 504, 536, 542
NlaIV GGNNCC 1 cut(s) 544
NmuCI GTSAC 1 cut(s) 404
NspV TTCGAA 1 cut(s) 11
PdmI GAANNNNTTC 2 cut(s) 17, 471
PfeI GAWTC 1 cut(s) 64
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 2 cut(s) 118, 139
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 532
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 1 cut(s) 525
PsuI RGATCY 2 cut(s) 536, 542
RsaI GTAC 2 cut(s) 6, 459
RsaNI GTAC 2 cut(s) 5, 458
SacII CCGCGG 1 cut(s) 141
SaqAI TTAA 4 cut(s) 216, 227, 414, 519
SatI GCNGC 2 cut(s) 117, 138
Sau3AI GATC 4 cut(s) 484, 504, 536, 542
Sau96I GGNCC 1 cut(s) 525
SchI GAGTC 1 cut(s) 382
ScrFI CCNGG 3 cut(s) 361, 426, 534
SfaNI GCATC 1 cut(s) 486
Sfr303I CCGCGG 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 11
SgrBI CCGCGG 1 cut(s) 141
SinI GGWCC 1 cut(s) 525
Sse9I AATT 1 cut(s) 202
SsiI CCGC 6 cut(s) 31, 107, 116, 138, 140, 313
SspMI CTAG 3 cut(s) 89, 120, 401
StyD4I CCNGG 3 cut(s) 359, 424, 532
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 3 cut(s) 197, 281, 297
TaiI ACGT 3 cut(s) 308, 424, 474
TaqI TCGA 5 cut(s) 11, 99, 191, 318, 507
TasI AATT 1 cut(s) 202
TauI GCSGC 2 cut(s) 119, 140
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 4 cut(s) 216, 227, 414, 519
Tru9I TTAA 4 cut(s) 216, 227, 414, 519
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 164, 467
TspRI CASTG 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 525
XapI RAATTY 1 cut(s) 202
XmnI GAANNNNTTC 2 cut(s) 17, 471
XspI CTAG 3 cut(s) 89, 120, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.