Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0010712

Physical Location & Seq

Genomic Coordinates Forward (+)

42554682 .. 42568751

Gene Structure 14070 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0278000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4911 bp

ATGAGTCTCACTGCTCTTGACATTTCCCTTGAAGGATCACAATCTCCCCATGATATCAAGCATTTCGTCCCCAGAAGGTATCAAGTGAAAGTGTTCGAAGTCGCAAAGCGTAAGAACACAATTGCAGTGTTGGAAACTGGTGCTGGGAAGACTATGATTGCTGTGATGCTCATCAAACACATTGCTGATCAACTCATCATCAAGTCCATTGGCCACAAGAAGTTGATAATTTTCATGGCCCCAACAGTTCATCTTGTCATTCAGCAATATGAGGTTATAAAAAAGTCTACAACTCTTGAAGTGGGAGTATACTACGGAGCCAAGGGCGTTGATGACTGGAGTCCAGAATGTTGGGAGGTGGAAGTGAATACACATGATATAATGGTCATGACACCTCAAATTCTCCTGGATGCTTTGCGGAATGCATTCTTGAAGATAGAGATGATTTGCTTGATGGTCTTCGATGAATGCCACCGTGCCACTGGCAATCATCCCTACGCGAAAATAATGAAGGAATTTCATCACAAATCAGGAAACAAGCCAAAGATTTTTGGAATGACAGCATCACCAGTCATTAAAAAAGGTGTCTCATCCGCCACGGACTGTGAGGATCAGATATCACAACTTGAAAGCATCTTGGATTCCCAGGTTTATACTGTAGAAGACAGGACAGAGATTGAAGAGCATGTTCCATCAGCAGTCCAAATTTGTAAATTTTATGACCCGACATGGTTAACCTGTGTGGATTTGAAAGCAAAGATGGAAGCTTTATGGTCAAAGTTTGATGCTTCTATCATGTCAAAGTTGCAAGGGCCAGAGAAAGGCGAGTACGAAGACATAAATGATAAAATCAAGATGCTACGAAAGAGGTTGTCCAATGACTACATGAAAATCTTGTATTGCCTTGATGATCTTGGTATCGTATGCGCATACGAGGCTGTGAAAGTCTGTCTGGAGAATGCCCCTGATGTCAAAGAGGAGTGCACATTTTATAGAGAAAGCTCTTTGCAATGCAGATATTTTCTTGATGAAGTCTTAAATATGATCAGTGAATTTCTTCAAAAAGGTTATATACTTCATACAGATTTTGAGTCAGACTATATAAAGGCACACAAAGTGGGATACATATCTCCGAAGTTACATGAACTTCTTCAAATTTTCCACTCATTTGGGAGCGATATGCAAGTTCTGTGCCTTATTTTCGTAGAGAGAATTGTCACAGCCAAAGTAATGCAAAGATATGTGAAGAAACTGCCATGTTTATCTCATTTCACAGCTTCATATTTGACCGGAAGTACCACCTCAGTTGATGCACTTGCCCCGAAATTGCAGAAGGAAATTTTGGAGTCATTTTACAGTGGGAAGGTCAATCTACTATTTACCACTGATGTAGTTGAGGAGGGAATTCATGTCCAGAACTGCTCCTGTGTGATCCGGTTTGATCTTCCCAAGACAGTTCGTAGCTATGTCCAGTCTCGAGGACGAGCACGAAAAAATGATTCCCAATATATCATAATGCTTGAAAGGGACAAGAAGAAGCAAAGAGACCATCTCTTTGACATAATCAGGAGTGAGTACTCAATGACAGATACGTCGAGAAATCGAGATCCTGATGGATGCAATTTAAGAGCTTGTAACTTTGAGGATGCAAATGCATACTATGTAGAAGCTACTGGAGCATCAATTACTCCAGACTCTAGTGTCAGCCTCATGTATCGGTACTGTGAAAAGCTCCCTGGAGATAGGTACTTTATTCCAAGGCCAAATTTTCACTTCAGACTATTTGAAGGGTTGTATCAGTGTGAAATAACATTACCTCCAAATGCGGCTTTTCAAACACTAGTTGGTCCTATGAGTAGAAACGGTCATTCATCAAAGCAGCTTGTATGCTTGGAAGCTTGTAAAATGCTGCATAAAATGGGTGCTTTGGATGATCATCTTCTCCCACATGTCGAAGTGCCTCTTGATAATGAAGTGATTGTGAAAAGCAAAGAATTATCTGCAGGTGCTGGAACGACAAAAAGGAAAGAGTTACATGGGACGACTTGCATTCATGCATTAAGTGGAACTTGGGGAGAGAAAGTTGATGGTGCCAGTTTTGAGGCCTACAAATTTGAGTTCTCCTGTAATGTTGATGAGACATATTCAGGATTTGTACTACTAGTCGAGTTGAAACTTGATGATGACGTGGGAAATACTGAAGTGGATCTCTACTTAATTGCAAAGACGGTCAAAGCTTACGTTTCTTCATGTGGAAAGGTGCATCTCAGTGCTGATCAGATAAAGGAAGCCATGTACTTTCAGGAATTTTTCTTTAATGGTTTGTTTGGGAGATTATTTCTTGGATCCGAAGCAAAAGGGACAGAAAGAAAATTTCTACTTCAGACTGAAATAAAATCACTATGGAGCTCATTGTACAGCTATTTACTTTTACCGACTGAGGCATCTGACAGTTCAAATAAGAAATCTTGGAACATAGATTGGACGGGAATCTGTTCCTGTGCTAATGTGGTGGAGTTTCTTAGGAAACACTATTCCAATGGTCTTGAGCATTTCAAAGGCGGTAGAGGAAATATATTACTTTCTAGGACTGGTTCATCCATGACAGATTGCAAGGCTGAAAATTTAGTCCAGTTTGCTAATAGTTCAATTGACGTCAATGATCTTGAAGATGTGGTAGTATTGGCTATTCACACAGGAAGAATCTACTCTGTTATTGAAGTTGTTAGTAGCATTTCTGCTGAGAGTCCCTTGGAAGAAAATGCTGATGCTGCCTCATCAGATTATACTTATGTCAAGTATGGGATTGTACTCAAGTATCCTGGACATCCTCTATTGCGGTTGAAGCAAAGTCATAATGCACATAACCTGCTTGTTGATCAAGGTGCGTCATCACCAGATGGTCTAGTTCAAGAAAAGCAACAAGCTCATGTTCATATGCCACCTGAAATCTTAGTCAGTATTGATGTTCGAGTTGACGTTCTAAAATCATTTTACTTACTACCATCACTAATGTATCGTCTGGAGTCCCTAATGTTAGGCAGCCAAATTAGGAAAGAGATCAATGGCCAGTCTAGCAACTTTTATATATCAAGCTCATTGATTCTAGAAGCACTCACAACACTTAGATGCTGTGAAAATTTTTCATTGGAGCGTTTGGAGTTGCTTGGAGATTCAGTTCTCAAATATGCTGTGAGTTCCTACCTCTTTCTTAAATATCCCGAGAAACATGAAGGACAATTAACTGCTCGACGAACAAGAGCTATTTGCAACTCAAACCTGCATAAACTAGGAACGAAAAACAAGTTACAGGGATACATACGAGACAGTGCATTTGAACCACGACGTTGGGCTGCTCCAGGGCAGCTTTCTAGATTTCCAGATCCATGTCAATGCGGAGTGGATACTTTGGAAGTGCCCTTGGACAGTAGATTTCAAACTGAAAAAGCTGTCAAGGTTGGAAAATTCTGTGATAAAGGTCACAGATGGATGAACTCAAAAACCATTGCTGATTGTGTTGAGGCCCTCATAGGAGCTTATTATGTTGGTGGAGGATTGTTTGCTGCATTTCATTTGATGAAGTGGCTCAGCATTGATTCTGAAACCGGACCCTCATTAGCTGATGAAGCCATTACAAGGGCATCACTTCGATGCTACAATCCCAAAACTAAGGAACTTGCTAACTTGGAGTTGAATCTTAGCTATGAATTTTCAGTCAAGGGCTTGTTACATGAGGCTGTCACACATGCGTCTCAGCAAAAACTAGGTGCCAGCTACTGTTATCAGAGGCTTGAATTTCTTGGAGACTCTGTGTTGGATCTGCTAACTACACAGCATCTCTATCGCAGCCACACAGATATTGATCCTGGATTGTTGACTGATTTGCGGTCAGCTGCTGTTAGTAATGAAAACTTTGCTCAAGCTGCTATTAGACAAAACCTTCATCCACATCTTCAACATTGCTCTGGGTTACTTCGGAGTCACATAACCGAGTATGAGAGGTTGCTTACAGAAGCTGAGAACAGTACCAGCTCACTGGAAGACATTAAGGGTCCAAAGGCTCTTGGTGACATGGTGGAAAGTATAGCAGGAGCTATACTAATTGATACAAAGCTTAATCTTGATGAAGTATGGAGGGTCTTTAAACCAATATTGTCTCCAATTGTGACCCCTTCTAGTCTTCAATTGCATCCGCTACGTAAACTGTCTGAATTATGTGATTCACTTGGGTACTTTGTGAAAGAAACATGTACTAAAGATGATACACTGGTGCATGTGGAACTAAGTTTGCAGCTGGAAGATCTTCTTTTGGTTGGAAACGGCTTTGACCGGAGTAGAAAAGCTGCGAAACATAAGGCAGCTCGTCAAGTGTTGAAGGCACTCGAGAAAAGCATAGAAAGGAGAAAGCATTATTCTGATATGGATAGTGACATCTGCAGCAACCTAACTGATGAAGATTTTTCAGAGCCAATGAACCATGGAAAGCAGAATATGACAACAATTCAATCATCTTCAGAGTCAAAAACAATAGATCCTACCCAATCCAACGACTGTTCTAATGAAGTCTTAAGTCACAACATTGCAACCCCAGTTATTGGTCCAATCGATACGAAAAAAGGTGAACCTCGAAAATCTCTCTTTGAGGTATGTAAGAAATTGCAGTGGCCAATGCCTGCATTCACAACAACAGAGCAGAAGTCAAGAACTCCAATTGAATTCGGTGAGGGTTCTGAAAAGAGAATAGGTTTCAGCAGCTTTCAATCAAAAATCAGCTTGCACATACCCAACTCTTGCACTATTGTATGCAAAGGAGATCCTAGAGCCGATAAGAAGACTTCCATGGATTCTGCAGGTGTTTCCATGCTTCACGAGCTTCAACGACGGAGAAAACTTGTCATTCTCCAGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0043170 GO:0043565 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901568 GO:1901576 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2

Protein Analysis

1636

Amino Acids

184.26

Weight (kDa)

6.41

Isoelectric Point (pI)

42.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	24 - 190	1e-15	Type III restriction enzyme, res subunit
DEAD	PF00270	26 - 190	7.8e-18	DEAD/DEAH box helicase
Helicase_C	PF00271	383 - 496	2.8e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	567 - 650	1.2e-22	Dicer dimerisation domain
Ribonuclease_3	PF00636	1051 - 1184	2.4e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1051 - 1108	2.9e-07	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	1241 - 1369	2.8e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1262 - 1370	5.2e-20	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11190 AT5G25390 AT5G25390
fragaria_vesca	FvH4_2g29150
malus_domestica	MD08G1149200.v1.1 MD15G1124900.v1.1
prunus_persica	Prupe.1G480400_v2.0.a1
pyrus_communis	pycom08g12560 pycom15g11250
rosa_chinensis	RchiOBHm_Chr6g0298011
rosa_laevigata	RLG00000011543
rosa_multiflora	Rmu_sc0002800.1_g000022
rosa_roxburghii	Rroxscaffold_7G00170000 Rroxscaffold_7G00178830
rosa_rugosa	Rorug06G0278000
rosa_samantha	Rh6AG388300 Rh6BG396500 Rh6CG402500 Rh6DG388700
rosa_wichuraiana	Rw6G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	278
AarI	CACCTGC	2 cut(s)	1994, 4841
AasI	GACNNNNNNGTC	2 cut(s)	1591, 1700
AatII	GACGTC	1 cut(s)	2658
Acc16I	TGCGCA	1 cut(s)	928
Acc36I	ACCTGC	4 cut(s)	1994, 2877, 3288, 4841
AccB1I	GGYRCC	2 cut(s)	2090, 3767
AccB7I	CCANNNNNTGG	1 cut(s)	4595
AccI	GTMKAC	2 cut(s)	287, 309
AccII	CGCG	1 cut(s)	500
AciI	CCGC	8 cut(s)	418, 594, 1826, 2562, 2839, 3396, 3886, 4193
AcoI	YGGCCR	3 cut(s)	211, 3067, 4664
AcuI	CTGAAG	4 cut(s)	1759, 2220, 2366, 4497
AcyI	GRCGYC	1 cut(s)	2655
AdeI	CACNNNGTG	1 cut(s)	1117
AfiI	CCNNNNNNNGG	3 cut(s)	821, 3636, 4595
AflII	CTTAAG	1 cut(s)	4567
AflIII	ACRYGT	2 cut(s)	1948, 4247
AhlI	ACTAGT	2 cut(s)	1840, 2161
AjiI	CACGTC	1 cut(s)	2188
AjnI	CCWGG	6 cut(s)	405, 645, 1735, 2821, 3358, 3865
AjuI	GAANNNNNNNTTGG	2 cut(s)	1325, 1357
AloI	GAACNNNNNNTCC	2 cut(s)	3162, 3194
Alw21I	GWGCWC	3 cut(s)	986, 1489, 2411
Alw26I	GTCTC	9 cut(s)	11, 592, 1479, 1539, 2132, 3318, 3756, 3798, 4161
Alw44I	GTGCAC	1 cut(s)	982
AlwNI	CAGNNNCTG	4 cut(s)	2009, 3777, 3896, 4016
Ama87I	CYCGRG	3 cut(s)	1476, 3221, 4382
AoxI	GGCC	8 cut(s)	211, 237, 812, 1760, 2103, 3067, 3522, 4664
ApaLI	GTGCAC	1 cut(s)	982
ArsI	GACNNNNNNTTYG	4 cut(s)	1538, 1570, 3317, 3349
Asp700I	GAANNNNTTC	6 cut(s)	92, 425, 1056, 1149, 2494, 4302
AspLEI	GCGC	1 cut(s)	929
AspS9I	GGNCC	7 cut(s)	238, 812, 1847, 3523, 3608, 4052, 4598
AsuHPI	GGTGA	5 cut(s)	558, 2886, 4079, 4631, 4733
AsuII	TTCGAA	1 cut(s)	96
AvaI	CYCGRG	3 cut(s)	1476, 3221, 4382
AvaII	GGWCC	4 cut(s)	1847, 3608, 4052, 4598
BaeGI	GKGCMC	2 cut(s)	986, 3420
BaeI	ACNNNNGTAYC	6 cut(s)	2801, 2834, 2999, 3032, 4254, 4287
BalI	TGGCCA	3 cut(s)	213, 3069, 4666
BamHI	GGATCC	1 cut(s)	2345
BanI	GGYRCC	2 cut(s)	2090, 3767
BanII	GRGCYC	1 cut(s)	2411
BauI	CACGAG	1 cut(s)	4868
BbsI	GAAGAC	7 cut(s)	155, 451, 669, 840, 4047, 4172, 4838
Bbv12I	GWGCWC	3 cut(s)	986, 1489, 2411
BccI	CCATC	9 cut(s)	448, 700, 754, 1557, 1607, 2081, 2894, 3013, 3480
BceAI	ACGGC	1 cut(s)	4336
BcgI	CGANNNNNNTGC	2 cut(s)	3824, 3858
BciT130I	CCWGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
BciVI	GTATCC	4 cut(s)	1115, 2829, 3308, 3397
BclI	TGATCA	5 cut(s)	187, 1044, 1933, 2275, 2878
BcoDI	GTCTC	9 cut(s)	11, 592, 1479, 1539, 2132, 3318, 3756, 3798, 4161
BcuI	ACTAGT	2 cut(s)	1840, 2161
BfmI	CTRYAG	4 cut(s)	657, 2001, 4435, 4848
BfrI	CTTAAG	1 cut(s)	4567
BfuAI	ACCTGC	4 cut(s)	1994, 2877, 3288, 4841
BfuI	GTATCC	4 cut(s)	1115, 2829, 3308, 3397
BglII	AGATCT	1 cut(s)	4300
BlpI	GCTNAGC	1 cut(s)	3587
BmcAI	AGTACT	1 cut(s)	1577
Bme1390I	CCNGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
Bme18I	GGWCC	4 cut(s)	1847, 3608, 4052, 4598
BmeT110I	CYCGRG	3 cut(s)	1476, 3221, 4382
BmgBI	CACGTC	1 cut(s)	2188
BmgT120I	GGNCC	7 cut(s)	238, 812, 1847, 3523, 3608, 4052, 4598
BmiI	GGNNCC	7 cut(s)	240, 319, 2092, 2347, 3610, 3769, 4053
BmrFI	CCNGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
BmrI	ACTGGG	1 cut(s)	4583
BmuI	ACTGGG	1 cut(s)	4583
BoxI	GACNNNNGTC	1 cut(s)	339
BpiI	GAAGAC	7 cut(s)	155, 451, 669, 840, 4047, 4172, 4838
BplI	GAGNNNNNCTC	2 cut(s)	1536, 1568
BpmI	CTGGAG	8 cut(s)	358, 974, 1674, 1695, 1758, 3044, 3342, 4886
Bpu1102I	GCTNAGC	1 cut(s)	3587
Bpu14I	TTCGAA	1 cut(s)	96
BpuEI	CTTGAG	3 cut(s)	2567, 2798, 3903
Bsa29I	ATCGAT	1 cut(s)	4605
BsaAI	YACGTR	1 cut(s)	4199
BsaBI	GATNNNNATC	5 cut(s)	40, 170, 1126, 1935, 3861
BsaHI	GRCGYC	1 cut(s)	2655
BsaI	GGTCTC	1 cut(s)	1539
BsaWI	WCCGGW	4 cut(s)	1289, 1434, 3605, 4329
BsaXI	ACNNNNNCTCC	8 cut(s)	1801, 1831, 2067, 2097, 3162, 3192, 3795, 3825
Bsc4I	CCNNNNNNNGG	3 cut(s)	821, 3636, 4595
Bse3DI	GCAATG	5 cut(s)	180, 1016, 3504, 3958, 4578
Bse8I	GATNNNNATC	5 cut(s)	40, 170, 1126, 1935, 3861
BseBI	CCWGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
BseCI	ATCGAT	1 cut(s)	4605
BseJI	GATNNNNATC	5 cut(s)	40, 170, 1126, 1935, 3861
BseLI	CCNNNNNNNGG	3 cut(s)	821, 3636, 4595
BseMI	GCAATG	5 cut(s)	180, 1016, 3504, 3958, 4578
BseMII	CTCAG	7 cut(s)	1317, 2281, 2430, 2733, 3601, 3767, 4008
BseRI	GAGGAG	2 cut(s)	992, 1412
BseSI	GKGCMC	2 cut(s)	986, 3420
BseYI	CCCAGC	1 cut(s)	143
Bsh1236I	CGCG	1 cut(s)	500
BshFI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3069, 3524, 4666
BshNI	GGYRCC	2 cut(s)	2090, 3767
BshVI	ATCGAT	1 cut(s)	4605
BsiHKAI	GWGCWC	3 cut(s)	986, 1489, 2411
BsiHKCI	CYCGRG	3 cut(s)	1476, 3221, 4382
BsiSI	CCGG	4 cut(s)	1290, 1435, 3606, 4330
BslFI	GGGAC	6 cut(s)	53, 1541, 2053, 2374, 2733, 3013
BslI	CCNNNNNNNGG	3 cut(s)	821, 3636, 4595
BsmAI	GTCTC	9 cut(s)	11, 592, 1479, 1539, 2132, 3318, 3756, 3798, 4161
BsmBI	CGTCTC	1 cut(s)	3756
BsmFI	GGGAC	6 cut(s)	53, 1541, 2053, 2374, 2733, 3013
BsmI	GAATGC	6 cut(s)	425, 427, 473, 964, 2049, 4676
BsnI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3069, 3524, 4666
Bso31I	GGTCTC	1 cut(s)	1539
BsoBI	CYCGRG	3 cut(s)	1476, 3221, 4382
Bsp119I	TTCGAA	1 cut(s)	96
Bsp1286I	GDGCHC	4 cut(s)	986, 1489, 2411, 3420
Bsp1407I	TGTACA	1 cut(s)	2415
Bsp1720I	GCTNAGC	1 cut(s)	3587
Bsp19I	CCATGG	2 cut(s)	4477, 4839
BspACI	CCGC	8 cut(s)	418, 594, 1826, 2562, 2839, 3396, 3886, 4193
BspANI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3069, 3524, 4666
BspCNI	CTCAG	7 cut(s)	1316, 2280, 2431, 2734, 3600, 3766, 4009
BspDI	ATCGAT	1 cut(s)	4605
BspFNI	CGCG	1 cut(s)	500
BspHI	TCATGA	1 cut(s)	387
BspLI	GGNNCC	7 cut(s)	240, 319, 2092, 2347, 3610, 3769, 4053
BspMAI	CTGCAG	3 cut(s)	2005, 4439, 4852
BspMI	ACCTGC	4 cut(s)	1994, 2877, 3288, 4841
BspQI	GCTCTTC	1 cut(s)	675
BspT104I	TTCGAA	1 cut(s)	96
BspT107I	GGYRCC	2 cut(s)	2090, 3767
BspTI	CTTAAG	1 cut(s)	4567
BspTNI	GGTCTC	1 cut(s)	1539
BsrDI	GCAATG	5 cut(s)	180, 1016, 3504, 3958, 4578
BsrGI	TGTACA	1 cut(s)	2415
BssNAI	GTATAC	1 cut(s)	310
BssNI	GRCGYC	1 cut(s)	2655
BssSI	CACGAG	1 cut(s)	4868
BssT1I	CCWWGG	6 cut(s)	321, 1757, 2751, 3420, 4477, 4839
Bst1107I	GTATAC	1 cut(s)	310
Bst2BI	CACGAG	1 cut(s)	4868
Bst2UI	CCWGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
Bst6I	CTCTTC	1 cut(s)	675
BstACI	GRCGYC	1 cut(s)	2655
BstAFI	CTTAAG	1 cut(s)	4567
BstAUI	TGTACA	1 cut(s)	2415
BstBAI	YACGTR	1 cut(s)	4199
BstBI	TTCGAA	1 cut(s)	96
BstC8I	GCNNGC	3 cut(s)	3772, 4674, 4775
BstDSI	CCRYGG	3 cut(s)	597, 4477, 4839
BstFNI	CGCG	1 cut(s)	500
BstHHI	GCGC	1 cut(s)	929
BstMAI	GTCTC	9 cut(s)	11, 592, 1479, 1539, 2132, 3318, 3756, 3798, 4161
BstMWI	GCNNNNNNNGC	8 cut(s)	935, 1676, 2771, 2845, 3075, 3626, 3923, 4870
BstNI	CCWGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
BstNSI	RCATGY	5 cut(s)	689, 1952, 3749, 4251, 4277
BstPAI	GACNNNNGTC	1 cut(s)	339
BstSCI	CCNGG	6 cut(s)	405, 645, 1735, 2821, 3358, 3865
BstSFI	CTRYAG	4 cut(s)	657, 2001, 4435, 4848
BstSLI	GKGCMC	2 cut(s)	986, 3420
BstSNI	TACGTA	1 cut(s)	4199
BstUI	CGCG	1 cut(s)	500
BstV2I	GAAGAC	7 cut(s)	155, 451, 669, 840, 4047, 4172, 4838
BstX2I	RGATCY	8 cut(s)	1606, 2206, 2345, 3382, 3817, 4300, 4531, 4813
BstXI	CCANNNNNNTGG	4 cut(s)	351, 1169, 3348, 4036
BstYI	RGATCY	8 cut(s)	1606, 2206, 2345, 3382, 3817, 4300, 4531, 4813
BstZ17I	GTATAC	1 cut(s)	310
Bsu15I	ATCGAT	1 cut(s)	4605
BsuI	GTATCC	4 cut(s)	1115, 2829, 3308, 3397
BsuRI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3069, 3524, 4666
BsuTUI	ATCGAT	1 cut(s)	4605
BtgI	CCRYGG	3 cut(s)	597, 4477, 4839
BtrI	CACGTC	1 cut(s)	2188
BtsI	GCAGTG	3 cut(s)	9, 132, 4667
BveI	ACCTGC	4 cut(s)	1994, 2877, 3288, 4841
Cac8I	GCNNGC	3 cut(s)	3772, 4674, 4775
CaiI	CAGNNNCTG	4 cut(s)	2009, 3777, 3896, 4016
CciI	TCATGA	1 cut(s)	387
CfoI	GCGC	1 cut(s)	929
Cfr13I	GGNCC	7 cut(s)	238, 812, 1847, 3523, 3608, 4052, 4598
ClaI	ATCGAT	1 cut(s)	4605
CseI	GACGC	2 cut(s)	2877, 3738
CspCI	CAANNNNNGTGG	2 cut(s)	1288, 1323
DraI	TTTAAA	1 cut(s)	4144
DraIII	CACNNNGTG	1 cut(s)	1117
DrdI	GACNNNNNNGTC	2 cut(s)	1591, 1700
DseDI	GACNNNNNNGTC	2 cut(s)	1591, 1700
EaeI	YGGCCR	3 cut(s)	211, 3067, 4664
Eam1104I	CTCTTC	1 cut(s)	675
EarI	CTCTTC	1 cut(s)	675
EciI	GGCGGA	1 cut(s)	583
Ecl136II	GAGCTC	1 cut(s)	2409
Eco105I	TACGTA	1 cut(s)	4199
Eco130I	CCWWGG	6 cut(s)	321, 1757, 2751, 3420, 4477, 4839
Eco147I	AGGCCT	1 cut(s)	2105
Eco24I	GRGCYC	1 cut(s)	2411
Eco31I	GGTCTC	1 cut(s)	1539
Eco32I	GATATC	2 cut(s)	55, 618
Eco47I	GGWCC	4 cut(s)	1847, 3608, 4052, 4598
Eco53kI	GAGCTC	1 cut(s)	2409
Eco57I	CTGAAG	4 cut(s)	1759, 2220, 2366, 4497
Eco88I	CYCGRG	3 cut(s)	1476, 3221, 4382
EcoICRI	GAGCTC	1 cut(s)	2409
EcoO109I	RGGNCCY	1 cut(s)	3523
EcoRI	GAATTC	2 cut(s)	1404, 4715
EcoRII	CCWGG	6 cut(s)	405, 645, 1735, 2821, 3358, 3865
EcoRV	GATATC	2 cut(s)	55, 618
EcoT14I	CCWWGG	6 cut(s)	321, 1757, 2751, 3420, 4477, 4839
EcoT22I	ATGCAT	3 cut(s)	427, 1657, 2059
EcoT38I	GRGCYC	1 cut(s)	2411
ErhI	CCWWGG	6 cut(s)	321, 1757, 2751, 3420, 4477, 4839
Esp3I	CGTCTC	1 cut(s)	3756
FalI	AAGNNNNNCTT	6 cut(s)	1947, 1979, 2053, 2085, 4290, 4322
FaqI	GGGAC	6 cut(s)	53, 1541, 2053, 2374, 2733, 3013
FauNDI	CATATG	1 cut(s)	2937
FbaI	TGATCA	5 cut(s)	187, 1044, 1933, 2275, 2878
FblI	GTMKAC	2 cut(s)	287, 309
FriOI	GRGCYC	1 cut(s)	2411
FspAI	RTGCGCAY	1 cut(s)	928
FspI	TGCGCA	1 cut(s)	928
GlaI	GCGC	1 cut(s)	928
GsaI	CCCAGC	1 cut(s)	147
GsuI	CTGGAG	8 cut(s)	358, 974, 1674, 1695, 1758, 3044, 3342, 4886
HaeIII	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3069, 3524, 4666
HapII	CCGG	4 cut(s)	1290, 1435, 3606, 4330
HgaI	GACGC	2 cut(s)	2877, 3738
HhaI	GCGC	1 cut(s)	929
Hin1I	GRCGYC	1 cut(s)	2655
Hin6I	GCGC	1 cut(s)	927
HinP1I	GCGC	1 cut(s)	927
HincII	GTYRAC	3 cut(s)	735, 2977, 3876
HindII	GTYRAC	3 cut(s)	735, 2977, 3876
HindIII	AAGCTT	4 cut(s)	765, 1896, 2235, 4112
HpaI	GTTAAC	1 cut(s)	735
HpaII	CCGG	4 cut(s)	1290, 1435, 3606, 4330
HphI	GGTGA	5 cut(s)	558, 2886, 4079, 4631, 4733
Hpy166II	GTNNAC	8 cut(s)	288, 310, 735, 984, 2977, 3876, 4202, 4622
Hpy8I	GTNNAC	8 cut(s)	288, 310, 735, 984, 2977, 3876, 4202, 4622
Hpy99I	CGWCG	4 cut(s)	1597, 3255, 3348, 4884
HpyCH4IV	ACGT	7 cut(s)	1592, 2187, 2241, 2655, 2979, 3346, 4198
HpyF10VI	GCNNNNNNNGC	8 cut(s)	935, 1676, 2771, 2845, 3075, 3626, 3923, 4870
HpySE526I	ACGT	7 cut(s)	1592, 2187, 2241, 2655, 2979, 3346, 4198
Hsp92I	GRCGYC	1 cut(s)	2655
HspAI	GCGC	1 cut(s)	927
Ksp22I	TGATCA	5 cut(s)	187, 1044, 1933, 2275, 2878
KspAI	GTTAAC	1 cut(s)	735
LguI	GCTCTTC	1 cut(s)	675
MaeII	ACGT	7 cut(s)	1592, 2187, 2241, 2655, 2979, 3346, 4198
MfeI	CAATTG	5 cut(s)	120, 2649, 4161, 4184, 4710
MflI	RGATCY	8 cut(s)	1606, 2206, 2345, 3382, 3817, 4300, 4531, 4813
MhlI	GDGCHC	4 cut(s)	986, 1489, 2411, 3420
MlsI	TGGCCA	3 cut(s)	213, 3069, 4666
MluNI	TGGCCA	3 cut(s)	213, 3069, 4666
MmeI	TCCRAC	5 cut(s)	111, 3439, 3795, 4294, 4569
Mox20I	TGGCCA	3 cut(s)	213, 3069, 4666
Mph1103I	ATGCAT	3 cut(s)	427, 1657, 2059
MroXI	GAANNNNTTC	6 cut(s)	92, 425, 1056, 1149, 2494, 4302
MscI	TGGCCA	3 cut(s)	213, 3069, 4666
MslI	CAYNNNNRTG	4 cut(s)	2268, 3127, 3391, 3649
Msp20I	TGGCCA	3 cut(s)	213, 3069, 4666
MspA1I	CMGCKG	2 cut(s)	3893, 4294
MspCI	CTTAAG	1 cut(s)	4567
MspI	CCGG	4 cut(s)	1290, 1435, 3606, 4330
MspR9I	CCNGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
MunI	CAATTG	5 cut(s)	120, 2649, 4161, 4184, 4710
Mva1269I	GAATGC	6 cut(s)	425, 427, 473, 964, 2049, 4676
MvaI	CCWGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
MvnI	CGCG	1 cut(s)	500
MwoI	GCNNNNNNNGC	8 cut(s)	935, 1676, 2771, 2845, 3075, 3626, 3923, 4870
NcoI	CCATGG	2 cut(s)	4477, 4839
NdeI	CATATG	1 cut(s)	2937
NlaIV	GGNNCC	7 cut(s)	240, 319, 2092, 2347, 3610, 3769, 4053
NmuCI	GTSAC	8 cut(s)	1216, 3479, 3739, 3980, 4067, 4165, 4427, 4571
NsbI	TGCGCA	1 cut(s)	928
NsiI	ATGCAT	3 cut(s)	427, 1657, 2059
NspI	RCATGY	5 cut(s)	689, 1952, 3749, 4251, 4277
NspV	TTCGAA	1 cut(s)	96
PaeR7I	CTCGAG	2 cut(s)	1476, 4382
PagI	TCATGA	1 cut(s)	387
PaqCI	CACCTGC	2 cut(s)	1994, 4841
PceI	AGGCCT	1 cut(s)	2105
PciI	ACATGT	2 cut(s)	1948, 4247
PciSI	GCTCTTC	1 cut(s)	675
PctI	GAATGC	6 cut(s)	425, 427, 473, 964, 2049, 4676
PdmI	GAANNNNTTC	6 cut(s)	92, 425, 1056, 1149, 2494, 4302
PflMI	CCANNNNNTGG	1 cut(s)	4595
PfoI	TCCNGGA	3 cut(s)	405, 2821, 3865
Ppu21I	YACGTR	1 cut(s)	4199
PscI	ACATGT	2 cut(s)	1948, 4247
PshAI	GACNNNNGTC	1 cut(s)	339
PsiI	TTATAA	1 cut(s)	278
Psp124BI	GAGCTC	1 cut(s)	2411
Psp6I	CCWGG	6 cut(s)	405, 645, 1735, 2821, 3358, 3865
PspFI	CCCAGC	1 cut(s)	143
PspGI	CCWGG	6 cut(s)	405, 645, 1735, 2821, 3358, 3865
PspN4I	GGNNCC	7 cut(s)	240, 319, 2092, 2347, 3610, 3769, 4053
PspPI	GGNCC	7 cut(s)	238, 812, 1847, 3523, 3608, 4052, 4598
PstI	CTGCAG	3 cut(s)	2005, 4439, 4852
PstNI	CAGNNNCTG	4 cut(s)	2009, 3777, 3896, 4016
PsuI	RGATCY	8 cut(s)	1606, 2206, 2345, 3382, 3817, 4300, 4531, 4813
PvuII	CAGCTG	2 cut(s)	3893, 4294
RseI	CAYNNNNRTG	4 cut(s)	2268, 3127, 3391, 3649
SacI	GAGCTC	1 cut(s)	2411
SapI	GCTCTTC	1 cut(s)	675
Sau96I	GGNCC	7 cut(s)	238, 812, 1847, 3523, 3608, 4052, 4598
ScaI	AGTACT	1 cut(s)	1577
ScrFI	CCNGG	6 cut(s)	407, 647, 1737, 2823, 3360, 3867
SduI	GDGCHC	4 cut(s)	986, 1489, 2411, 3420
SfcI	CTRYAG	4 cut(s)	657, 2001, 4435, 4848
Sfr274I	CTCGAG	2 cut(s)	1476, 4382
SfuI	TTCGAA	1 cut(s)	96
SinI	GGWCC	4 cut(s)	1847, 3608, 4052, 4598
SlaI	CTCGAG	2 cut(s)	1476, 4382
SmiMI	CAYNNNNRTG	4 cut(s)	2268, 3127, 3391, 3649
SmlI	CTYRAG	6 cut(s)	1476, 2546, 2813, 3918, 4382, 4567
SmoI	CTYRAG	6 cut(s)	1476, 2546, 2813, 3918, 4382, 4567
SnaBI	TACGTA	1 cut(s)	4199
SpeI	ACTAGT	2 cut(s)	1840, 2161
SseBI	AGGCCT	1 cut(s)	2105
SsiI	CCGC	8 cut(s)	418, 594, 1826, 2562, 2839, 3396, 3886, 4193
SspI	AATATT	1 cut(s)	4152
SstI	GAGCTC	1 cut(s)	2411
StuI	AGGCCT	1 cut(s)	2105
StyD4I	CCNGG	6 cut(s)	405, 645, 1735, 2821, 3358, 3865
StyI	CCWWGG	6 cut(s)	321, 1757, 2751, 3420, 4477, 4839
TaiI	ACGT	7 cut(s)	1595, 2190, 2244, 2658, 2982, 3349, 4201
TatI	WGTACW	6 cut(s)	1575, 2155, 2295, 2415, 2809, 4250
TauI	GCSGC	1 cut(s)	1829
TseFI	GTSAC	8 cut(s)	1216, 3479, 3739, 3980, 4067, 4165, 4427, 4571
Tsp45I	GTSAC	8 cut(s)	1216, 3479, 3739, 3980, 4067, 4165, 4427, 4571
TspGWI	ACGGA	3 cut(s)	330, 614, 4897
Van91I	CCANNNNNTGG	1 cut(s)	4595
Vha464I	CTTAAG	1 cut(s)	4567
VneI	GTGCAC	1 cut(s)	982
VpaK11BI	GGWCC	4 cut(s)	1847, 3608, 4052, 4598
XbaI	TCTAGA	2 cut(s)	3106, 3371
XceI	RCATGY	5 cut(s)	689, 1952, 3749, 4251, 4277
XcmI	CCANNNNNNNNNTGG	1 cut(s)	479
XhoI	CTCGAG	2 cut(s)	1476, 4382
XmiI	GTMKAC	2 cut(s)	287, 309
XmnI	GAANNNNTTC	6 cut(s)	92, 425, 1056, 1149, 2494, 4302
ZraI	GACGTC	1 cut(s)	2656
ZrmI	AGTACT	1 cut(s)	1577
Zsp2I	ATGCAT	3 cut(s)	427, 1657, 2059

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.