FvH4_2g29150

Ethylene-responsive transcription factor SHINE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010712
Physical Location & Seq
Forward (+)
22677466 .. 22679189
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29150.t1

Sequence Viewer

Length: 699 bp
ATGGTACAATCAAGGAAGTTCAGAGGAGTCAGGCAGCGCCAGTGGGGCTCCTGGGTGTCAGAGATTCGCCACCCATTACTTAAGAGGAGGGTGTGGCTAGGCACATTTGAGACAGCTGAGGCAGCAGCAAGAGCATATGACCAAGCATCCATATTGATGAATGGTCAGAATGCCAAGACCAATTTTCCCAATAACAACCCCCCATCTGATGATCAAGAAACAAAAGCTGCTGATCATCTTCCTCACGGTCATCAGCTTCCATGGGACCCCAAAGAACTTGCAGAGCTGCTCAGTTCCAAGCTCAAGAAGTGTTGCAAAGACCCATCTCCTTCCCTCACTTGTCTGAGGCTCGACAACGACAATTCTCACATTGGAGTTTGGCAGAAGAGACCCGCAGGATCCCGTGCGAGCTCTAACTGGGTCATGAGAATTGAGCTGGGGAAGAAGAAGAAGCAATGTAACGGTACAGTTACTGAACATGAAGATGGATCGAAATCAACATCATCATCATCATCGTCATCTCTTATAAGTCAATCATCAGCTGGATCAAATACTAACGAGGCTGGAATTGCAATTGAAGAAGCTGATGAGGATCATGGTGAAGAGGATGAGCTTGCAATGCAGATGATAGAGGAATTACTCAACTGGAATAGTTATCCAATTCCTTCACCTACAAGTAATGTTCAAGAAGGGAATTAA

Protein Analysis

233

Amino Acids

25.77

Weight (kDa)

6.0

Isoelectric Point (pI)

55.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 6 - 55 8.7e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010712)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11190 AT5G25390 AT5G25390
fragaria_vesca FvH4_2g29150
malus_domestica MD08G1149200.v1.1 MD15G1124900.v1.1
prunus_persica Prupe.1G480400_v2.0.a1
pyrus_communis pycom08g12560 pycom15g11250
rosa_chinensis RchiOBHm_Chr6g0298011
rosa_laevigata RLG00000011543
rosa_multiflora Rmu_sc0002800.1_g000022
rosa_roxburghii Rroxscaffold_7G00170000 Rroxscaffold_7G00178830
rosa_rugosa Rorug06G0278000
rosa_samantha Rh6AG388300 Rh6BG396500 Rh6CG402500 Rh6DG388700
rosa_wichuraiana Rw6G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AciI CCGC 1 cut(s) 393
AclWI GGATC 5 cut(s) 393, 406, 496, 553, 600
AfaI GTAC 2 cut(s) 6, 466
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 2 cut(s) 578, 686
AjnI CCWGG 1 cut(s) 50
Alw21I GWGCWC 1 cut(s) 413
Alw26I GTCTC 2 cut(s) 104, 382
AlwI GGATC 5 cut(s) 393, 406, 496, 553, 600
AlwNI CAGNNNCTG 1 cut(s) 473
ApeKI GCWGC 5 cut(s) 34, 122, 125, 227, 286
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 2 cut(s) 611, 660
AvaII GGWCC 1 cut(s) 265
BamHI GGATCC 1 cut(s) 398
BanII GRGCYC 2 cut(s) 50, 413
Bbv12I GWGCWC 1 cut(s) 413
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 5 cut(s) 46, 134, 137, 214, 273
BccI CCATC 3 cut(s) 211, 331, 479
BciT130I CCWGG 1 cut(s) 52
BclI TGATCA 2 cut(s) 211, 232
BcoDI GTCTC 2 cut(s) 104, 382
BfaI CTAG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 40
BfrI CTTAAG 1 cut(s) 80
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 5 cut(s) 35, 123, 126, 228, 287
BlsI GCNGC 5 cut(s) 36, 124, 127, 229, 288
Bme1390I CCNGG 1 cut(s) 52
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 4 cut(s) 49, 266, 267, 400
BmrFI CCNGG 1 cut(s) 52
BmrI ACTGGG 1 cut(s) 427
BmsI GCATC 1 cut(s) 155
BmuI ACTGGG 1 cut(s) 427
Bpu10I CCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 287
BsaBI GATNNNNATC 2 cut(s) 493, 591
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 2 cut(s) 51, 260
Bse1I ACTGG 3 cut(s) 40, 422, 650
Bse3DI GCAATG 2 cut(s) 461, 624
Bse8I GATNNNNATC 2 cut(s) 493, 591
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 2 cut(s) 51, 260
BseGI GGATG 2 cut(s) 146, 613
BseJI GATNNNNATC 2 cut(s) 493, 591
BseMI GCAATG 2 cut(s) 461, 624
BseMII CTCAG 3 cut(s) 108, 304, 335
BseNI ACTGG 3 cut(s) 40, 422, 650
BseRI GAGGAG 2 cut(s) 39, 100
BseXI GCAGC 5 cut(s) 46, 134, 137, 214, 273
BseYI CCCAGC 1 cut(s) 436
BsiHKAI GWGCWC 1 cut(s) 413
BslFI GGGAC 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 104, 382
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 175
Bso31I GGTCTC 1 cut(s) 382
Bsp1286I GDGCHC 2 cut(s) 50, 413
Bsp143I GATC 6 cut(s) 211, 232, 398, 488, 545, 592
Bsp19I CCATGG 1 cut(s) 260
BspACI CCGC 1 cut(s) 393
BspCNI CTCAG 3 cut(s) 109, 303, 336
BspHI TCATGA 1 cut(s) 423
BspLI GGNNCC 4 cut(s) 49, 266, 267, 400
BspPI GGATC 5 cut(s) 393, 406, 496, 553, 600
BspTI CTTAAG 1 cut(s) 80
BspTNI GGTCTC 1 cut(s) 382
BsrDI GCAATG 2 cut(s) 461, 624
BsrI ACTGG 3 cut(s) 40, 422, 650
BssECI CCNNGG 2 cut(s) 51, 260
BssMI GATC 6 cut(s) 211, 232, 398, 488, 545, 592
BssT1I CCWWGG 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 3 cut(s) 248, 464, 469
Bst6I CTCTTC 2 cut(s) 380, 597
BstAFI CTTAAG 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 409, 615
BstDEI CTNAG 3 cut(s) 117, 290, 344
BstDSI CCRYGG 1 cut(s) 260
BstF5I GGATG 2 cut(s) 146, 613
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 6 cut(s) 214, 235, 401, 491, 548, 595
BstMAI GTCTC 2 cut(s) 104, 382
BstMBI GATC 6 cut(s) 211, 232, 398, 488, 545, 592
BstMWI GCNNNNNNNGC 5 cut(s) 45, 122, 131, 569, 619
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 50
BstV1I GCAGC 5 cut(s) 46, 134, 137, 214, 273
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BtgI CCRYGG 1 cut(s) 260
BtsCI GGATG 2 cut(s) 146, 613
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 2 cut(s) 409, 615
CaiI CAGNNNCTG 1 cut(s) 473
CciI TCATGA 1 cut(s) 423
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 5, 465
CviAII CATG 4 cut(s) 261, 424, 479, 596
CviQI GTAC 2 cut(s) 5, 465
DdeI CTNAG 3 cut(s) 117, 290, 344
DpnI GATC 6 cut(s) 213, 234, 400, 490, 547, 594
DpnII GATC 6 cut(s) 211, 232, 398, 488, 545, 592
Eam1104I CTCTTC 2 cut(s) 380, 597
EarI CTCTTC 2 cut(s) 380, 597
Ecl136II GAGCTC 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 260
Eco24I GRGCYC 2 cut(s) 50, 413
Eco31I GGTCTC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 411
EcoO109I RGGNCCY 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 260
EcoT38I GRGCYC 2 cut(s) 50, 413
ErhI CCWWGG 1 cut(s) 260
FaeI CATG 4 cut(s) 264, 427, 482, 599
FaiI YATR 8 cut(s) 136, 138, 152, 262, 425, 480, 527, 597
FaqI GGGAC 1 cut(s) 278
FatI CATG 4 cut(s) 260, 423, 478, 595
FauI CCCGC 1 cut(s) 400
FauNDI CATATG 1 cut(s) 136
FbaI TGATCA 2 cut(s) 211, 232
Fnu4HI GCNGC 5 cut(s) 35, 123, 126, 228, 287
FokI GGATG 2 cut(s) 133, 620
FriOI GRGCYC 2 cut(s) 50, 413
Fsp4HI GCNGC 5 cut(s) 35, 123, 126, 228, 287
FspBI CTAG 1 cut(s) 98
GlaI GCGC 1 cut(s) 38
GluI GCNGC 5 cut(s) 35, 123, 126, 228, 287
GsaI CCCAGC 1 cut(s) 440
HaeII RGCGCY 1 cut(s) 40
HhaI GCGC 1 cut(s) 39
Hin1II CATG 4 cut(s) 264, 427, 482, 599
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 2 cut(s) 27, 64
HphI GGTGA 2 cut(s) 611, 660
Hpy188I TCNGA 5 cut(s) 23, 61, 168, 208, 345
Hpy188III TCNNGA 4 cut(s) 215, 304, 424, 686
HpyAV CCTTC 3 cut(s) 339, 675, 683
HpyCH4III ACNGT 3 cut(s) 248, 464, 469
HpyCH4V TGCA 5 cut(s) 281, 315, 572, 617, 622
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 122, 131, 569, 619
HpyF3I CTNAG 3 cut(s) 117, 290, 344
Hsp92II CATG 4 cut(s) 264, 427, 482, 599
HspAI GCGC 1 cut(s) 37
KflI GGGWCCC 1 cut(s) 265
Ksp22I TGATCA 2 cut(s) 211, 232
Kzo9I GATC 6 cut(s) 211, 232, 398, 488, 545, 592
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 5 cut(s) 46, 134, 137, 214, 273
LweI GCATC 1 cut(s) 155
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 458, 469
MalI GATC 6 cut(s) 213, 234, 400, 490, 547, 594
MboI GATC 6 cut(s) 211, 232, 398, 488, 545, 592
MboII GAAGA 8 cut(s) 230, 397, 454, 457, 460, 494, 590, 614
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 2 cut(s) 50, 413
MluCI AATT 8 cut(s) 181, 361, 429, 567, 573, 635, 660, 694
MlyI GAGTC 1 cut(s) 36
MseI TTAA 2 cut(s) 81, 697
MslI CAYNNNNRTG 2 cut(s) 155, 483
MspA1I CMGCKG 2 cut(s) 116, 542
MspCI CTTAAG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 5 cut(s) 45, 122, 131, 569, 619
NcoI CCATGG 1 cut(s) 260
NdeI CATATG 1 cut(s) 136
NdeII GATC 6 cut(s) 211, 232, 398, 488, 545, 592
NlaIII CATG 4 cut(s) 264, 427, 482, 599
NlaIV GGNNCC 4 cut(s) 49, 266, 267, 400
PagI TCATGA 1 cut(s) 423
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 64
PkrI GCNGC 5 cut(s) 36, 124, 127, 229, 288
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 265
PsiI TTATAA 1 cut(s) 527
Psp124BI GAGCTC 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 436
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 4 cut(s) 49, 266, 267, 400
PspPI GGNCC 1 cut(s) 265
PspPPI RGGWCCY 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 1 cut(s) 398
PvuII CAGCTG 2 cut(s) 116, 542
RsaI GTAC 2 cut(s) 6, 466
RsaNI GTAC 2 cut(s) 5, 465
RseI CAYNNNNRTG 2 cut(s) 155, 483
SacI GAGCTC 1 cut(s) 413
SaqAI TTAA 2 cut(s) 81, 697
SatI GCNGC 5 cut(s) 35, 123, 126, 228, 287
Sau3AI GATC 6 cut(s) 211, 232, 398, 488, 545, 592
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 2 cut(s) 50, 413
SfaNI GCATC 1 cut(s) 155
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 155, 483
SmlI CTYRAG 2 cut(s) 80, 302
SmoI CTYRAG 2 cut(s) 80, 302
Sse9I AATT 8 cut(s) 181, 361, 429, 567, 573, 635, 660, 694
SsiI CCGC 1 cut(s) 393
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 3 cut(s) 248, 464, 469
TaqI TCGA 2 cut(s) 351, 491
TasI AATT 8 cut(s) 181, 361, 429, 567, 573, 635, 660, 694
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 2 cut(s) 81, 697
Tru9I TTAA 2 cut(s) 81, 697
TscAI CASTG 1 cut(s) 47
TseI GCWGC 5 cut(s) 34, 122, 125, 227, 286
TspDTI ATGAA 2 cut(s) 173, 495
TspRI CASTG 1 cut(s) 47
Vha464I CTTAAG 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 265
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.