AT5G25410

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017110
Physical Location & Seq
Forward (+)
8831670 .. 8833509
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25410.1

Sequence Viewer

Length: 1110 bp
ATGTCTACAAGAGTTAGGATTGCTCTTGTTATGAATGTCTTCCTTTTTTGGTTCTCACCAGCCCTAATTCGTTCGGATCCAAATATGGTGCCTCTAAGAACCTTCAAGATACGTGAAAATGTAACATATGACTGCATTAACATTTATAAGCAACCAGGACTTGATCATCCCTTGCTCAAAAACCATAAAATTCAGATGAAACCATCAGTTTCAAGACATGAATTAAGGAATCAAACAGACAACACTAAAACATATAAGAATAAAATGGGATGTCCAGATGGAACCGTTCCTATATTAAGAAATTCAAAAGAATATATTACCAATGCACAACTTTTCGCCGAGAAGTATTTTCATCCGCTATCAAGCGAAAGTCCTGGATCACATGTTGCTGGAGTAAGGTCACAAAATGGTCCATATCATGGTGTAGATGCTTCATTTAGTATACATAAATTAAACATCGAAAGAGATCAGGCCTCATATGCTCAATTATATGTAGGCAGTGGTTTGAACCATCAGATCAATTTTATCCAAGCAGGTTGGATGATAAATCCAAGTATATTTGGCGATGGAGGTGTTTGGTCATATGGATTTTGGAAGGGTGAAAATGGAAATGGATGTTATAATACGGCATGTCCAGGGTTTGTTCAAGTATCACAGGAGGTTCCCATAGCCCAGCCCTTGGACCAGCCAAAGGAAGATTTGTTGCATTACTCCATCCATCAGGATAAACAAACAGGAAACTGGTGGATTACAAAACTGATAGCAAATGCACCTAACATAGATGTCGGATATTGGCCAAAAGAATTATTTAACCTAATAGGTAATGGTGCAAATATGGTGGGAGTTGGAGGTGCAGTTCAAGCTTCTCATCAAGGTCCAAGTCCTCCGATGGGTAATGGTAAATTTCCGATTGGAGACCCAAAGGAATCAGCAATGTTTACAAATATTGAAGTCTTGAATTCCAACTATGAACAACGTCGAATCGATTCTTTTCCTATGGAGAAGTTGCTAGATAGCCCCAAATGTTATGGAATAAATACCGATAAAATAAAACTACTCGGTTTTGCTTTTAATTATGGTGGTGCGGGAGGAGAATTTTGTGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

369

Amino Acids

41.12

Weight (kDa)

8.39

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 42 - 112 8.4e-19 Neprosin activation peptide
Neprosin PF03080 156 - 361 1.1e-58 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04937 AT5G05030 AT5G11660 AT5G25410 AT5G25415 AT5G36680 AT5G36770 AT5G60380
fragaria_vesca FvH4_2g09090 FvH4_4g25910
malus_domestica MD10G1076000.v1.1 MD10G1207400.v1.1
pyrus_communis pycom02g18760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 147, 621
Acc36I ACCTGC 1 cut(s) 524
AccB1I GGYRCC 1 cut(s) 88
AccB7I CCANNNNNTGG 2 cut(s) 419, 679
AccI GTMKAC 2 cut(s) 5, 442
AciI CCGC 2 cut(s) 356, 1085
AclWI GGATC 3 cut(s) 71, 84, 385
AcoI YGGCCR 1 cut(s) 794
AcsI RAATTY 5 cut(s) 189, 301, 902, 958, 1094
AfiI CCNNNNNNNGG 4 cut(s) 419, 679, 691, 890
AflIII ACRYGT 1 cut(s) 382
AgsI TTSAA 8 cut(s) 106, 213, 306, 508, 647, 860, 950, 958
AjnI CCWGG 3 cut(s) 154, 373, 634
AloI GAACNNNNNNTCC 2 cut(s) 840, 872
AluBI AGCT 1 cut(s) 863
AluI AGCT 1 cut(s) 863
Alw26I GTCTC 1 cut(s) 909
AlwI GGATC 3 cut(s) 71, 84, 385
AoxI GGCC 2 cut(s) 471, 794
ApoI RAATTY 5 cut(s) 189, 301, 902, 958, 1094
Asp700I GAANNNNTTC 2 cut(s) 38, 985
AspS9I GGNCC 3 cut(s) 410, 682, 875
AsuHPI GGTGA 2 cut(s) 48, 611
AvaII GGWCC 3 cut(s) 410, 682, 875
BalI TGGCCA 1 cut(s) 796
BamHI GGATCC 1 cut(s) 76
BanI GGYRCC 1 cut(s) 88
BbsI GAAGAC 1 cut(s) 31
BccI CCATC 7 cut(s) 211, 272, 519, 560, 722, 726, 883
BceAI ACGGC 1 cut(s) 642
BciT130I CCWGG 3 cut(s) 156, 375, 636
BclI TGATCA 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 909
BfaI CTAG 1 cut(s) 1010
BfuAI ACCTGC 1 cut(s) 524
Bme1390I CCNGG 3 cut(s) 156, 375, 636
Bme18I GGWCC 3 cut(s) 410, 682, 875
BmgT120I GGNCC 3 cut(s) 410, 682, 875
BmiI GGNNCC 4 cut(s) 78, 90, 283, 663
BmrFI CCNGG 3 cut(s) 156, 375, 636
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 984
BsaAI YACGTR 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 909
BsaJI CCNNGG 2 cut(s) 635, 678
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 4 cut(s) 419, 679, 691, 890
Bse1I ACTGG 1 cut(s) 746
Bse3DI GCAATG 1 cut(s) 939
BseBI CCWGG 3 cut(s) 156, 375, 636
BseCI ATCGAT 1 cut(s) 984
BseDI CCNNGG 2 cut(s) 635, 678
BseGI GGATG 6 cut(s) 166, 275, 352, 546, 620, 714
BseLI CCNNNNNNNGG 4 cut(s) 419, 679, 691, 890
BseMI GCAATG 1 cut(s) 939
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 1 cut(s) 1104
BseYI CCCAGC 1 cut(s) 672
BsgI GTGCAG 1 cut(s) 873
BshFI GGCC 2 cut(s) 473, 796
BshNI GGYRCC 1 cut(s) 88
BshVI ATCGAT 1 cut(s) 984
BslI CCNNNNNNNGG 4 cut(s) 419, 679, 691, 890
BsmAI GTCTC 1 cut(s) 909
BsnI GGCC 2 cut(s) 473, 796
Bso31I GGTCTC 1 cut(s) 909
Bsp143I GATC 5 cut(s) 76, 163, 377, 466, 516
BspACI CCGC 2 cut(s) 356, 1085
BspANI GGCC 2 cut(s) 473, 796
BspDI ATCGAT 1 cut(s) 984
BspLI GGNNCC 4 cut(s) 78, 90, 283, 663
BspMI ACCTGC 1 cut(s) 524
BspPI GGATC 3 cut(s) 71, 84, 385
BspT107I GGYRCC 1 cut(s) 88
BspTNI GGTCTC 1 cut(s) 909
BsrDI GCAATG 1 cut(s) 939
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 2 cut(s) 635, 678
BssMI GATC 5 cut(s) 76, 163, 377, 466, 516
BssNAI GTATAC 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 678
Bst1107I GTATAC 1 cut(s) 443
Bst2UI CCWGG 3 cut(s) 156, 375, 636
Bst4CI ACNGT 1 cut(s) 286
BstBAI YACGTR 1 cut(s) 113
BstDEI CTNAG 1 cut(s) 95
BstF5I GGATG 6 cut(s) 166, 275, 352, 546, 620, 714
BstKTI GATC 5 cut(s) 79, 166, 380, 469, 519
BstMAI GTCTC 1 cut(s) 909
BstMBI GATC 5 cut(s) 76, 163, 377, 466, 516
BstMWI GCNNNNNNNGC 2 cut(s) 479, 860
BstNI CCWGG 3 cut(s) 156, 375, 636
BstNSI RCATGY 2 cut(s) 386, 633
BstSCI CCNGG 3 cut(s) 154, 373, 634
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BstZ17I GTATAC 1 cut(s) 443
Bsu15I ATCGAT 1 cut(s) 984
BsuRI GGCC 2 cut(s) 473, 796
BsuTUI ATCGAT 1 cut(s) 984
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 6 cut(s) 166, 275, 352, 546, 620, 714
BtsI GCAGTG 1 cut(s) 505
BtsIMutI CAGTG 1 cut(s) 505
BveI ACCTGC 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 410, 682, 875
ClaI ATCGAT 1 cut(s) 984
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviAII CATG 4 cut(s) 218, 383, 419, 630
CviJI RGCY 8 cut(s) 62, 473, 671, 676, 688, 796, 863, 1017
CviKI_1 RGCY 8 cut(s) 62, 473, 671, 676, 688, 796, 863, 1017
DdeI CTNAG 1 cut(s) 95
DpnI GATC 5 cut(s) 78, 165, 379, 468, 518
DpnII GATC 5 cut(s) 76, 163, 377, 466, 516
EaeI YGGCCR 1 cut(s) 794
Eco130I CCWWGG 1 cut(s) 678
Eco147I AGGCCT 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 909
Eco47I GGWCC 3 cut(s) 410, 682, 875
EcoRI GAATTC 1 cut(s) 958
EcoRII CCWGG 3 cut(s) 154, 373, 634
EcoT14I CCWWGG 1 cut(s) 678
ErhI CCWWGG 1 cut(s) 678
FaeI CATG 4 cut(s) 221, 386, 422, 633
FatI CATG 4 cut(s) 217, 382, 418, 629
FauI CCCGC 1 cut(s) 1078
FauNDI CATATG 3 cut(s) 127, 478, 583
FbaI TGATCA 1 cut(s) 163
FblI GTMKAC 2 cut(s) 5, 442
FokI GGATG 6 cut(s) 153, 282, 339, 553, 627, 701
FspBI CTAG 1 cut(s) 1010
GsaI CCCAGC 1 cut(s) 676
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 2 cut(s) 473, 796
Hin1II CATG 4 cut(s) 221, 386, 422, 633
HindIII AAGCTT 1 cut(s) 861
HinfI GANTC 4 cut(s) 229, 926, 981, 986
HphI GGTGA 2 cut(s) 48, 611
Hpy166II GTNNAC 3 cut(s) 6, 443, 939
Hpy188I TCNGA 6 cut(s) 76, 195, 516, 788, 888, 909
Hpy188III TCNNGA 5 cut(s) 106, 213, 275, 722, 955
Hpy8I GTNNAC 3 cut(s) 6, 443, 939
Hpy99I CGWCG 1 cut(s) 981
HpyAV CCTTC 2 cut(s) 112, 589
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 2 cut(s) 112, 976
HpyCH4V TGCA 6 cut(s) 135, 326, 706, 770, 830, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 860
HpyF3I CTNAG 1 cut(s) 95
HpySE526I ACGT 2 cut(s) 112, 976
Hsp92II CATG 4 cut(s) 221, 386, 422, 633
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 5 cut(s) 76, 163, 377, 466, 516
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 1010
MaeII ACGT 2 cut(s) 112, 976
MaeIII GTNAC 2 cut(s) 121, 399
MalI GATC 5 cut(s) 78, 165, 379, 468, 518
MboI GATC 5 cut(s) 76, 163, 377, 466, 516
MboII GAAGA 2 cut(s) 31, 707
MflI RGATCY 1 cut(s) 76
MlsI TGGCCA 1 cut(s) 796
MluNI TGGCCA 1 cut(s) 796
MmeI TCCRAC 4 cut(s) 518, 766, 826, 987
MnlI CCTC 7 cut(s) 102, 484, 563, 652, 842, 894, 1082
Mox20I TGGCCA 1 cut(s) 796
MroXI GAANNNNTTC 2 cut(s) 38, 985
MscI TGGCCA 1 cut(s) 796
MseI TTAA 6 cut(s) 138, 224, 296, 452, 810, 1071
Msp20I TGGCCA 1 cut(s) 796
MspR9I CCNGG 3 cut(s) 156, 375, 636
MvaI CCWGG 3 cut(s) 156, 375, 636
MwoI GCNNNNNNNGC 2 cut(s) 479, 860
NdeI CATATG 3 cut(s) 127, 478, 583
NdeII GATC 5 cut(s) 76, 163, 377, 466, 516
NlaIII CATG 4 cut(s) 221, 386, 422, 633
NlaIV GGNNCC 4 cut(s) 78, 90, 283, 663
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 399
NspI RCATGY 2 cut(s) 386, 633
PceI AGGCCT 1 cut(s) 473
PciI ACATGT 1 cut(s) 382
PdmI GAANNNNTTC 2 cut(s) 38, 985
PfeI GAWTC 4 cut(s) 229, 926, 981, 986
PflMI CCANNNNNTGG 2 cut(s) 419, 679
PfoI TCCNGGA 1 cut(s) 373
Ppu21I YACGTR 1 cut(s) 113
PscI ACATGT 1 cut(s) 382
PsiI TTATAA 2 cut(s) 147, 621
Psp6I CCWGG 3 cut(s) 154, 373, 634
PspFI CCCAGC 1 cut(s) 672
PspGI CCWGG 3 cut(s) 154, 373, 634
PspN4I GGNNCC 4 cut(s) 78, 90, 283, 663
PspPI GGNCC 3 cut(s) 410, 682, 875
PsuI RGATCY 1 cut(s) 76
SaqAI TTAA 6 cut(s) 138, 224, 296, 452, 810, 1071
Sau3AI GATC 5 cut(s) 76, 163, 377, 466, 516
Sau96I GGNCC 3 cut(s) 410, 682, 875
ScrFI CCNGG 3 cut(s) 156, 375, 636
SfaNI GCATC 1 cut(s) 418
SinI GGWCC 3 cut(s) 410, 682, 875
SseBI AGGCCT 1 cut(s) 473
SsiI CCGC 2 cut(s) 356, 1085
SspI AATATT 1 cut(s) 946
SspMI CTAG 1 cut(s) 1010
StuI AGGCCT 1 cut(s) 473
StyD4I CCNGG 3 cut(s) 154, 373, 634
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 2 cut(s) 115, 979
TaqI TCGA 3 cut(s) 459, 979, 984
TfiI GAWTC 4 cut(s) 229, 926, 981, 986
Tru1I TTAA 6 cut(s) 138, 224, 296, 452, 810, 1071
Tru9I TTAA 6 cut(s) 138, 224, 296, 452, 810, 1071
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 399
Tsp45I GTSAC 1 cut(s) 399
TspDTI ATGAA 6 cut(s) 47, 212, 234, 341, 423, 984
TspRI CASTG 1 cut(s) 505
Van91I CCANNNNNTGG 2 cut(s) 419, 679
VpaK11BI GGWCC 3 cut(s) 410, 682, 875
XapI RAATTY 5 cut(s) 189, 301, 902, 958, 1094
XceI RCATGY 2 cut(s) 386, 633
XmiI GTMKAC 2 cut(s) 5, 442
XmnI GAANNNNTTC 2 cut(s) 38, 985
XspI CTAG 1 cut(s) 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.