AT5G60380

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017110
Physical Location & Seq
Forward (+)
24285920 .. 24287808
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60380.1

Sequence Viewer

Length: 1122 bp
ATGTCTACAACAACTAGGATCACTCTTGTTATGAATCTTTTGCCTCTTTTGTTCTCAGTAGCTCTAGTTGGTTCGGTTCAATGTACGGTGCCTCTCAGATCCTTCAAGATAAGTGAAAATGTGACATACGATTGCATAGATATTTATAAACAACCAGGACTTGATCATTCCTTGCTCAAAAACCACACAATCCAGATGAAACCATCATTATCAAGACATGAGCTAAAGAATCAAACTAGCAACAACAAAACATATAAGAAGGACATAGAATGTCCATATGGAACTATTCCTGTATTGAGAAACACAAAAGAATTCAACACCAAAGCCCAACTCTTGGCTGCCAAGTATTTCAATCCGTTATCAGCTGATAGCCCTGGAACACATATCGCTGGGGTAAAGCAACACGGTGGTCCATATCATGGTATAGAAGCTAAGTTTAATGCATATAACCTAAACATTGGAGAAGATCAAGCCTCATATAGTCAAATGTATTTAGGCAGTGGACATTATGGCGAAGTCAATTTCATTTCAACGGGGATGATGATAAATCCAGGCATTTTTGGTGACGGACGTCTTTGGACATATGGATTTTGGATGGGTAAAGGTGGAAAAGGATGTTATAACATGGCATGTCCAGGGTTTGTTCAAGTATCGAATGTGGTTCCCTTAGTCAAACCCATGTACCTTAAGCCAGGGGAGCCTGCATCGTTGCAATGGGCCATCCATCAAGATGAACAAACACGAAACTGGTGGATTATACAAATGTCGCCTTATACATATATCGGTTATTGGCCAAAAGAGCTATTTTACCTTATGGATAATGGTGCTACTATGGTTGGAGTTGGAGGTGTAGTTCAGGCTTCACCTTCTGGTTTAAGCCCTCCAATGGGTAATGGTAAATTTCCGGCAAAAGGTCCTTTGAGATCAGCCATGTTTTCCAATGTTGATGTCTTATATTCCAAGTATGAGAAAGGTAAAATAAATGCGTTTCCTATAGTGGAGTTGCTAGATAGTAGTAGATGTTATGGGCTACGAATTGGTAAGAGAGTTCGATTTTGGACTAGTCCACTCGGATACTTTTTCAATTATGGTGGTCCTGGAGGAATCTCTTGTGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

373

Amino Acids

41.33

Weight (kDa)

9.22

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 42 - 109 5.1e-17 Neprosin activation peptide
Neprosin PF03080 156 - 366 4.6e-54 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04937 AT5G05030 AT5G11660 AT5G25410 AT5G25415 AT5G36680 AT5G36770 AT5G60380
fragaria_vesca FvH4_2g09090 FvH4_4g25910
malus_domestica MD10G1076000.v1.1 MD10G1207400.v1.1
pyrus_communis pycom02g18760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 147, 621
AatII GACGTC 1 cut(s) 574
AccB1I GGYRCC 1 cut(s) 88
AccB7I CCANNNNNTGG 2 cut(s) 334, 419
AccI GTMKAC 1 cut(s) 5
AclWI GGATC 2 cut(s) 26, 93
AcoI YGGCCR 1 cut(s) 791
AcsI RAATTY 2 cut(s) 311, 899
AcyI GRCGYC 1 cut(s) 571
AfaI GTAC 2 cut(s) 85, 683
AfiI CCNNNNNNNGG 5 cut(s) 334, 419, 886, 887, 911
AflII CTTAAG 1 cut(s) 686
AgsI TTSAA 7 cut(s) 80, 106, 316, 352, 531, 647, 1084
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 6 cut(s) 154, 373, 550, 634, 691, 1096
AloI GAACNNNNNNTCC 2 cut(s) 837, 869
AluBI AGCT 5 cut(s) 62, 223, 365, 431, 802
AluI AGCT 5 cut(s) 62, 223, 365, 431, 802
AlwI GGATC 2 cut(s) 26, 93
AoxI GGCC 2 cut(s) 717, 791
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 2 cut(s) 311, 899
AspS9I GGNCC 4 cut(s) 410, 717, 914, 1094
AsuHPI GGTGA 2 cut(s) 575, 855
AvaII GGWCC 3 cut(s) 410, 914, 1094
BaeI ACNNNNGTAYC 2 cut(s) 665, 698
BalI TGGCCA 1 cut(s) 793
BanI GGYRCC 1 cut(s) 88
BbvI GCAGC 1 cut(s) 325
BccI CCATC 4 cut(s) 211, 589, 728, 732
BciT130I CCWGG 6 cut(s) 156, 375, 552, 636, 693, 1098
BciVI GTATCC 1 cut(s) 1067
BclI TGATCA 1 cut(s) 163
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 5 cut(s) 15, 65, 237, 1007, 1062
BfmI CTRYAG 1 cut(s) 993
BfrI CTTAAG 1 cut(s) 686
BfuI GTATCC 1 cut(s) 1067
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 6 cut(s) 156, 375, 552, 636, 693, 1098
Bme18I GGWCC 3 cut(s) 410, 914, 1094
BmgT120I GGNCC 4 cut(s) 410, 717, 914, 1094
BmiI GGNNCC 3 cut(s) 90, 663, 699
BmrFI CCNGG 6 cut(s) 156, 375, 552, 636, 693, 1098
BmsI GCATC 1 cut(s) 713
BoxI GACNNNNGTC 1 cut(s) 570
BpmI CTGGAG 1 cut(s) 1119
BsaHI GRCGYC 1 cut(s) 571
BsaJI CCNNGG 3 cut(s) 373, 635, 692
BsaXI ACNNNNNCTCC 2 cut(s) 837, 867
Bsc4I CCNNNNNNNGG 5 cut(s) 334, 419, 886, 887, 911
Bse1I ACTGG 1 cut(s) 752
Bse3DI GCAATG 1 cut(s) 719
BseBI CCWGG 6 cut(s) 156, 375, 552, 636, 693, 1098
BseDI CCNNGG 3 cut(s) 373, 635, 692
BseGI GGATG 4 cut(s) 543, 600, 620, 720
BseLI CCNNNNNNNGG 5 cut(s) 334, 419, 886, 887, 911
BseMI GCAATG 1 cut(s) 719
BseMII CTCAG 2 cut(s) 69, 109
BseNI ACTGG 1 cut(s) 752
BseXI GCAGC 1 cut(s) 325
BseYI CCCAGC 1 cut(s) 389
BshFI GGCC 2 cut(s) 719, 793
BshNI GGYRCC 1 cut(s) 88
BsiSI CCGG 1 cut(s) 905
BslI CCNNNNNNNGG 5 cut(s) 334, 419, 886, 887, 911
BsnI GGCC 2 cut(s) 719, 793
Bsp143I GATC 5 cut(s) 18, 98, 163, 466, 923
BspANI GGCC 2 cut(s) 719, 793
BspCNI CTCAG 2 cut(s) 68, 108
BspLI GGNNCC 3 cut(s) 90, 663, 699
BspPI GGATC 2 cut(s) 26, 93
BspT107I GGYRCC 1 cut(s) 88
BspTI CTTAAG 1 cut(s) 686
BsrDI GCAATG 1 cut(s) 719
BsrI ACTGG 1 cut(s) 752
BssECI CCNNGG 3 cut(s) 373, 635, 692
BssMI GATC 5 cut(s) 18, 98, 163, 466, 923
BssNI GRCGYC 1 cut(s) 571
Bst2UI CCWGG 6 cut(s) 156, 375, 552, 636, 693, 1098
Bst4CI ACNGT 2 cut(s) 88, 407
BstACI GRCGYC 1 cut(s) 571
BstAFI CTTAAG 1 cut(s) 686
BstC8I GCNNGC 1 cut(s) 702
BstDEI CTNAG 4 cut(s) 55, 95, 432, 667
BstF5I GGATG 4 cut(s) 543, 600, 620, 720
BstKTI GATC 5 cut(s) 21, 101, 166, 469, 926
BstMBI GATC 5 cut(s) 18, 98, 163, 466, 923
BstMWI GCNNNNNNNGC 2 cut(s) 697, 799
BstNI CCWGG 6 cut(s) 156, 375, 552, 636, 693, 1098
BstNSI RCATGY 1 cut(s) 633
BstPAI GACNNNNGTC 1 cut(s) 570
BstSCI CCNGG 6 cut(s) 154, 373, 550, 634, 691, 1096
BstSFI CTRYAG 1 cut(s) 993
BstV1I GCAGC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BsuI GTATCC 1 cut(s) 1067
BsuRI GGCC 2 cut(s) 719, 793
BtsCI GGATG 4 cut(s) 543, 600, 620, 720
BtsI GCAGTG 1 cut(s) 505
BtsIMutI CAGTG 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 702
Cfr13I GGNCC 4 cut(s) 410, 717, 914, 1094
Csp6I GTAC 2 cut(s) 84, 682
CspCI CAANNNNNGTGG 2 cut(s) 1072, 1107
CviAII CATG 6 cut(s) 218, 419, 625, 630, 679, 931
CviQI GTAC 2 cut(s) 84, 682
DdeI CTNAG 4 cut(s) 55, 95, 432, 667
DpnI GATC 5 cut(s) 20, 100, 165, 468, 925
DpnII GATC 5 cut(s) 18, 98, 163, 466, 923
EaeI YGGCCR 1 cut(s) 791
Eco47I GGWCC 3 cut(s) 410, 914, 1094
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 6 cut(s) 154, 373, 550, 634, 691, 1096
EcoT22I ATGCAT 1 cut(s) 445
FaeI CATG 6 cut(s) 221, 422, 628, 633, 682, 934
FatI CATG 6 cut(s) 217, 418, 624, 629, 678, 930
FauNDI CATATG 2 cut(s) 277, 583
FbaI TGATCA 1 cut(s) 163
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 4 cut(s) 550, 607, 627, 707
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 5 cut(s) 15, 65, 237, 1007, 1062
GluI GCNGC 1 cut(s) 339
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 2 cut(s) 719, 793
HapII CCGG 1 cut(s) 905
Hin1I GRCGYC 1 cut(s) 571
Hin1II CATG 6 cut(s) 221, 422, 628, 633, 682, 934
HinfI GANTC 3 cut(s) 34, 229, 1104
HpaII CCGG 1 cut(s) 905
HphI GGTGA 2 cut(s) 575, 855
Hpy166II GTNNAC 3 cut(s) 6, 503, 1067
Hpy188I TCNGA 2 cut(s) 98, 1073
Hpy188III TCNNGA 4 cut(s) 106, 193, 213, 728
Hpy8I GTNNAC 3 cut(s) 6, 503, 1067
HpyAV CCTTC 3 cut(s) 112, 253, 876
HpyCH4III ACNGT 2 cut(s) 88, 407
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 4 cut(s) 135, 443, 704, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 697, 799
HpyF3I CTNAG 4 cut(s) 55, 95, 432, 667
HpySE526I ACGT 1 cut(s) 571
Hsp92I GRCGYC 1 cut(s) 571
Hsp92II CATG 6 cut(s) 221, 422, 628, 633, 682, 934
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 5 cut(s) 18, 98, 163, 466, 923
LmnI GCTCC 1 cut(s) 697
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 713
MaeI CTAG 5 cut(s) 15, 65, 237, 1007, 1062
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 2 cut(s) 121, 563
MalI GATC 5 cut(s) 20, 100, 165, 468, 925
MboI GATC 5 cut(s) 18, 98, 163, 466, 923
MboII GAAGA 1 cut(s) 476
MflI RGATCY 1 cut(s) 98
MlsI TGGCCA 1 cut(s) 793
MluCI AATT 5 cut(s) 311, 520, 899, 1035, 1084
MluNI TGGCCA 1 cut(s) 793
MmeI TCCRAC 2 cut(s) 817, 823
MnlI CCTC 6 cut(s) 54, 102, 484, 839, 891, 1094
Mox20I TGGCCA 1 cut(s) 793
Mph1103I ATGCAT 1 cut(s) 445
MscI TGGCCA 1 cut(s) 793
MseI TTAA 3 cut(s) 438, 687, 875
MslI CAYNNNNRTG 1 cut(s) 729
Msp20I TGGCCA 1 cut(s) 793
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 686
MspI CCGG 1 cut(s) 905
MspR9I CCNGG 6 cut(s) 156, 375, 552, 636, 693, 1098
MvaI CCWGG 6 cut(s) 156, 375, 552, 636, 693, 1098
MwoI GCNNNNNNNGC 2 cut(s) 697, 799
NdeI CATATG 2 cut(s) 277, 583
NdeII GATC 5 cut(s) 18, 98, 163, 466, 923
NlaIII CATG 6 cut(s) 221, 422, 628, 633, 682, 934
NlaIV GGNNCC 3 cut(s) 90, 663, 699
NmuCI GTSAC 2 cut(s) 121, 563
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 1 cut(s) 633
PfeI GAWTC 3 cut(s) 34, 229, 1104
PflMI CCANNNNNTGG 2 cut(s) 334, 419
PfoI TCCNGGA 1 cut(s) 1096
PkrI GCNGC 1 cut(s) 340
PpuMI RGGWCCY 1 cut(s) 914
PshAI GACNNNNGTC 1 cut(s) 570
PsiI TTATAA 2 cut(s) 147, 621
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 6 cut(s) 154, 373, 550, 634, 691, 1096
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 6 cut(s) 154, 373, 550, 634, 691, 1096
PspN4I GGNNCC 3 cut(s) 90, 663, 699
PspPI GGNCC 4 cut(s) 410, 717, 914, 1094
PspPPI RGGWCCY 1 cut(s) 914
PsuI RGATCY 1 cut(s) 98
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 85, 683
RsaNI GTAC 2 cut(s) 84, 682
RseI CAYNNNNRTG 1 cut(s) 729
SaqAI TTAA 3 cut(s) 438, 687, 875
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 5 cut(s) 18, 98, 163, 466, 923
Sau96I GGNCC 4 cut(s) 410, 717, 914, 1094
ScrFI CCNGG 6 cut(s) 156, 375, 552, 636, 693, 1098
SfaNI GCATC 1 cut(s) 713
SfcI CTRYAG 1 cut(s) 993
SinI GGWCC 3 cut(s) 410, 914, 1094
SmiMI CAYNNNNRTG 1 cut(s) 729
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
SpeI ACTAGT 1 cut(s) 1061
Sse9I AATT 5 cut(s) 311, 520, 899, 1035, 1084
SspMI CTAG 5 cut(s) 15, 65, 237, 1007, 1062
StyD4I CCNGG 6 cut(s) 154, 373, 550, 634, 691, 1096
TaaI ACNGT 2 cut(s) 88, 407
TaiI ACGT 1 cut(s) 574
TaqI TCGA 2 cut(s) 653, 1051
TasI AATT 5 cut(s) 311, 520, 899, 1035, 1084
TfiI GAWTC 3 cut(s) 34, 229, 1104
Tru1I TTAA 3 cut(s) 438, 687, 875
Tru9I TTAA 3 cut(s) 438, 687, 875
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 2 cut(s) 121, 563
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 2 cut(s) 121, 563
TspDTI ATGAA 4 cut(s) 47, 212, 514, 747
TspGWI ACGGA 2 cut(s) 345, 582
TspRI CASTG 1 cut(s) 505
Van91I CCANNNNNTGG 2 cut(s) 334, 419
Vha464I CTTAAG 1 cut(s) 686
VpaK11BI GGWCC 3 cut(s) 410, 914, 1094
XapI RAATTY 2 cut(s) 311, 899
XceI RCATGY 1 cut(s) 633
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 5 cut(s) 15, 65, 237, 1007, 1062
ZraI GACGTC 1 cut(s) 572
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.