FvH4_2g09090

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017110
Physical Location & Seq
Forward (+)
7993497 .. 7995505
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g09090.t1

Sequence Viewer

Length: 819 bp
ATGGCCAAAACTCTTTCGCATCACATATTCAATAACATCAGCAGTACTAATGATTTTGCCCCTAATAATCACAAAGTCTTCGTTAGACTAAACGAAACACGAGGTATCAGATCCTCTGGACTTTATGGAATGACTTCGGTATACAAACCAAGTGTGGCACCTGACCAGTCTACTGCTGGCACTATTTGGGTGGGAAGGGGTTTGGGTGACCAAACTAATCTTCTTATGGCAGGGTGGATGATTTCACATTCAATAAACGGTGATGATCTGCCTCGGTTGTTTGTGTATTGGACTGTAGATGGAGGCCATATAGGATGCTTTAATTTGCTTTGCCATGGATTTGTACAAGTAGATCGAAAATTTGCTCCCAAACGTACTAGGTTTGAGCCATTGTCTACTGTAGGTGGAAAAACATATGAGGTCAAGTTTGCAATTCACCAGGACCCCGTAACGGCAAATTGGTGGCTTACAATGACAGACTGGAATATTACAGTGGGGTATTGGCCAAAGGAAATATTACCTCATTTACGTGCAGGAGCAGGAGAAGTAGGGTGGGGAGGACTTACCATGGCCACCGGTAAAGATAGTCCTCCAATGGGAAGTGGTCAGTACCCAGATGGCACGACTAATCATGCTGCCTATTTCAGGAACTTACATTACTTGAGGGCTTGGAATAAACCACTAGTTCCGTCAGATAAGTATCCTGTGTTCGAGTTTATAGAATCAACTGGCTGTTATGGCTTACAGAATGATAAAGGTACTAGGCAGGGGTATTGGGGTTATAGCTTTCTCTACGGAGGACCAGGGGGCTCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

273

Amino Acids

30.22

Weight (kDa)

8.8

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 55 - 268 8.8e-59 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04937 AT5G05030 AT5G11660 AT5G25410 AT5G25415 AT5G36680 AT5G36770 AT5G60380
fragaria_vesca FvH4_2g09090 FvH4_4g25910
malus_domestica MD10G1076000.v1.1 MD10G1207400.v1.1
pyrus_communis pycom02g18760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccI GTMKAC 3 cut(s) 141, 170, 395
AclWI GGATC 1 cut(s) 105
AcoI YGGCCR 3 cut(s) 3, 503, 570
AcsI RAATTY 1 cut(s) 359
AfaI GTAC 5 cut(s) 46, 345, 376, 611, 760
AfiI CCNNNNNNNGG 3 cut(s) 451, 596, 645
AgeI ACCGGT 1 cut(s) 575
AgsI TTSAA 2 cut(s) 31, 252
AhlI ACTAGT 1 cut(s) 682
AjnI CCWGG 2 cut(s) 438, 802
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AluBI AGCT 1 cut(s) 786
AluI AGCT 1 cut(s) 786
AlwI GGATC 1 cut(s) 105
AoxI GGCC 4 cut(s) 3, 304, 503, 570
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 359
AsiGI ACCGGT 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 133
AspS9I GGNCC 2 cut(s) 442, 800
AsuHPI GGTGA 3 cut(s) 218, 272, 428
AvaII GGWCC 2 cut(s) 442, 800
BalI TGGCCA 3 cut(s) 5, 505, 572
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 812
BauI CACGAG 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 622
BccI CCATC 2 cut(s) 293, 611
BceAI ACGGC 1 cut(s) 468
BciT130I CCWGG 2 cut(s) 440, 804
BciVI GTATCC 1 cut(s) 711
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 3 cut(s) 378, 683, 762
BfmI CTRYAG 2 cut(s) 294, 399
BfuI GTATCC 1 cut(s) 711
BisI GCNGC 1 cut(s) 636
BlsI GCNGC 1 cut(s) 637
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 2 cut(s) 440, 804
Bme18I GGWCC 2 cut(s) 442, 800
BmgT120I GGNCC 2 cut(s) 442, 800
BmiI GGNNCC 2 cut(s) 159, 444
BmrFI CCNGG 2 cut(s) 440, 804
BmsI GCATC 2 cut(s) 28, 305
BpiI GAAGAC 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 682
BsaAI YACGTR 1 cut(s) 530
BsaBI GATNNNNATC 1 cut(s) 699
BsaJI CCNNGG 4 cut(s) 272, 334, 567, 803
BsaWI WCCGGW 1 cut(s) 575
Bsc4I CCNNNNNNNGG 3 cut(s) 451, 596, 645
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 3 cut(s) 166, 485, 733
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 2 cut(s) 440, 804
BseDI CCNNGG 4 cut(s) 272, 334, 567, 803
BseGI GGATG 2 cut(s) 243, 320
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 3 cut(s) 451, 596, 645
BseNI ACTGG 3 cut(s) 166, 485, 733
BseXI GCAGC 1 cut(s) 622
BsgI GTGCAG 1 cut(s) 552
BshFI GGCC 4 cut(s) 5, 306, 505, 572
BshNI GGYRCC 1 cut(s) 157
BshTI ACCGGT 1 cut(s) 575
BsiSI CCGG 1 cut(s) 576
BslI CCNNNNNNNGG 3 cut(s) 451, 596, 645
BsnI GGCC 4 cut(s) 5, 306, 505, 572
Bsp1286I GDGCHC 1 cut(s) 812
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 3 cut(s) 110, 265, 352
Bsp19I CCATGG 2 cut(s) 334, 567
BspANI GGCC 4 cut(s) 5, 306, 505, 572
BspLI GGNNCC 2 cut(s) 159, 444
BspPI GGATC 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 157
BsrFI RCCGGY 1 cut(s) 575
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 3 cut(s) 166, 485, 733
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 4 cut(s) 272, 334, 567, 803
BssMI GATC 3 cut(s) 110, 265, 352
BssNAI GTATAC 1 cut(s) 142
BssSI CACGAG 1 cut(s) 99
BssT1I CCWWGG 2 cut(s) 334, 567
Bst1107I GTATAC 1 cut(s) 142
Bst2BI CACGAG 1 cut(s) 99
Bst2UI CCWGG 2 cut(s) 440, 804
Bst4CI ACNGT 4 cut(s) 260, 295, 400, 493
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 178
BstDSI CCRYGG 2 cut(s) 334, 567
BstEII GGTNACC 1 cut(s) 206
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 2 cut(s) 243, 320
BstKTI GATC 3 cut(s) 113, 268, 355
BstMBI GATC 3 cut(s) 110, 265, 352
BstMWI GCNNNNNNNGC 1 cut(s) 738
BstNI CCWGG 2 cut(s) 440, 804
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 438, 802
BstSFI CTRYAG 2 cut(s) 294, 399
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 110
BstYI RGATCY 1 cut(s) 110
BstZ17I GTATAC 1 cut(s) 142
BsuI GTATCC 1 cut(s) 711
BsuRI GGCC 4 cut(s) 5, 306, 505, 572
BtgI CCRYGG 2 cut(s) 334, 567
BtsCI GGATG 2 cut(s) 243, 320
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 575
Cfr13I GGNCC 2 cut(s) 442, 800
Csp6I GTAC 5 cut(s) 45, 344, 375, 610, 759
CspAI ACCGGT 1 cut(s) 575
CviAII CATG 3 cut(s) 335, 568, 632
CviQI GTAC 5 cut(s) 45, 344, 375, 610, 759
DpnI GATC 3 cut(s) 112, 267, 354
DpnII GATC 3 cut(s) 110, 265, 352
EaeI YGGCCR 3 cut(s) 3, 503, 570
Eco130I CCWWGG 2 cut(s) 334, 567
Eco24I GRGCYC 1 cut(s) 812
Eco47I GGWCC 2 cut(s) 442, 800
Eco91I GGTNACC 1 cut(s) 206
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 442
EcoO65I GGTNACC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 438, 802
EcoT14I CCWWGG 2 cut(s) 334, 567
EcoT38I GRGCYC 1 cut(s) 812
ErhI CCWWGG 2 cut(s) 334, 567
FaeI CATG 3 cut(s) 338, 571, 635
FatI CATG 3 cut(s) 334, 567, 631
FauNDI CATATG 1 cut(s) 415
FblI GTMKAC 3 cut(s) 141, 170, 395
Fnu4HI GCNGC 1 cut(s) 636
FokI GGATG 2 cut(s) 250, 327
FriOI GRGCYC 1 cut(s) 812
Fsp4HI GCNGC 1 cut(s) 636
FspBI CTAG 3 cut(s) 378, 683, 762
GluI GCNGC 1 cut(s) 636
HaeIII GGCC 4 cut(s) 5, 306, 505, 572
HapII CCGG 1 cut(s) 576
Hin1II CATG 3 cut(s) 338, 571, 635
HinfI GANTC 1 cut(s) 722
HpaII CCGG 1 cut(s) 576
HphI GGTGA 3 cut(s) 218, 272, 428
Hpy166II GTNNAC 3 cut(s) 142, 171, 396
Hpy188I TCNGA 2 cut(s) 110, 694
Hpy188III TCNNGA 2 cut(s) 117, 646
Hpy8I GTNNAC 3 cut(s) 142, 171, 396
HpyAV CCTTC 1 cut(s) 189
HpyCH4III ACNGT 4 cut(s) 260, 295, 400, 493
HpyCH4IV ACGT 2 cut(s) 373, 529
HpyCH4V TGCA 2 cut(s) 431, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 738
HpySE526I ACGT 2 cut(s) 373, 529
Hsp92II CATG 3 cut(s) 338, 571, 635
Kzo9I GATC 3 cut(s) 110, 265, 352
LmnI GCTCC 2 cut(s) 370, 536
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 2 cut(s) 28, 305
MaeI CTAG 3 cut(s) 378, 683, 762
MaeII ACGT 2 cut(s) 373, 529
MaeIII GTNAC 2 cut(s) 206, 448
MalI GATC 3 cut(s) 112, 267, 354
MboI GATC 3 cut(s) 110, 265, 352
MboII GAAGA 2 cut(s) 70, 212
MflI RGATCY 1 cut(s) 110
MhlI GDGCHC 1 cut(s) 812
MlsI TGGCCA 3 cut(s) 5, 505, 572
MluCI AATT 4 cut(s) 322, 359, 432, 457
MluNI TGGCCA 3 cut(s) 5, 505, 572
Mox20I TGGCCA 3 cut(s) 5, 505, 572
MroXI GAANNNNTTC 1 cut(s) 133
MscI TGGCCA 3 cut(s) 5, 505, 572
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 528
Msp20I TGGCCA 3 cut(s) 5, 505, 572
MspI CCGG 1 cut(s) 576
MspR9I CCNGG 2 cut(s) 440, 804
MvaI CCWGG 2 cut(s) 440, 804
MwoI GCNNNNNNNGC 1 cut(s) 738
NcoI CCATGG 2 cut(s) 334, 567
NdeI CATATG 1 cut(s) 415
NdeII GATC 3 cut(s) 110, 265, 352
NlaIII CATG 3 cut(s) 338, 571, 635
NlaIV GGNNCC 2 cut(s) 159, 444
NmuCI GTSAC 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 722
PinAI ACCGGT 1 cut(s) 575
PkrI GCNGC 1 cut(s) 637
Ppu21I YACGTR 1 cut(s) 530
PpuMI RGGWCCY 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 2 cut(s) 438, 802
PspEI GGTNACC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 438, 802
PspN4I GGNNCC 2 cut(s) 159, 444
PspPI GGNCC 2 cut(s) 442, 800
PspPPI RGGWCCY 1 cut(s) 442
PsrI GAACNNNNNNTAC 4 cut(s) 641, 673, 692, 724
PsuI RGATCY 1 cut(s) 110
RsaI GTAC 5 cut(s) 46, 345, 376, 611, 760
RsaNI GTAC 5 cut(s) 45, 344, 375, 610, 759
RseI CAYNNNNRTG 1 cut(s) 528
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 1 cut(s) 636
Sau3AI GATC 3 cut(s) 110, 265, 352
Sau96I GGNCC 2 cut(s) 442, 800
ScaI AGTACT 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 440, 804
SduI GDGCHC 1 cut(s) 812
SfaNI GCATC 2 cut(s) 28, 305
SfcI CTRYAG 2 cut(s) 294, 399
SinI GGWCC 2 cut(s) 442, 800
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
SpeI ACTAGT 1 cut(s) 682
Sse9I AATT 4 cut(s) 322, 359, 432, 457
SspI AATATT 2 cut(s) 487, 516
SspMI CTAG 3 cut(s) 378, 683, 762
StyD4I CCNGG 2 cut(s) 438, 802
StyI CCWWGG 2 cut(s) 334, 567
TaaI ACNGT 4 cut(s) 260, 295, 400, 493
TaiI ACGT 2 cut(s) 376, 532
TaqI TCGA 2 cut(s) 355, 711
TasI AATT 4 cut(s) 322, 359, 432, 457
TatI WGTACW 2 cut(s) 44, 343
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 1 cut(s) 206
TspGWI ACGGA 2 cut(s) 678, 810
TspRI CASTG 1 cut(s) 498
VpaK11BI GGWCC 2 cut(s) 442, 800
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 1 cut(s) 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XmiI GTMKAC 3 cut(s) 141, 170, 395
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 3 cut(s) 378, 683, 762
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.