MD10G1076000.v1.1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017110
Physical Location & Seq
Forward (+)
10837402 .. 10838742
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1076000.v1.1.491

Sequence Viewer

Length: 759 bp
ATGCAGCAAGTATTCATTAGAATGAACGAAAGTCAGCTGGTAATTAGATACATTGGAGTTTTCGGGAGTGGGGCGATATTCAAACCAAACATGGCACAAGACCAATCTAGCTCTGTCAATGTTTGGGTCGATAGTGGCCCGCCTGACTACGTTAGCAGGCTTATTGCTGGTTCCTTTGTTTCAACATCAATCAATGGTGATGACAGCCCTCGAGTGTTTATATACTGGACTTTGGATGCGGATAAGACGGGATGCTTTGATTCGCTCTGCCAGGGATTCGTACAAATCGACCGAAACGTAGGTCCTAAAACTCGCTTTGAGCCAGTGTCATCTTTTGGTGGAGCAACATATGATGCCAAATTCGATATTTACCAGGATTATATAACAAAAAATTGGTGGCTTGTGATGACAGACAAAAACATTAGCGTAGGGTATTGGCCAAGGGAACTAGTTCCTAGTTTGGAAAATGGGTCTGGAGATGTGGGATGGGGAGGAATTGCCATGGCTCCAGAGAACGAAGATGGGCCCCCGATGGGGAGTGGCCAATACCCAGATGGCACGTATGCACATGTTGGGTACTTCAGAAACCTGCATTTCATGAAGGGTTGGCATGAAGGACTTGGATTGTCTGCCCTCCCATTTCGGTACTATTTCCGTGTTCTTCTGTTTTGTGTGGTCACAGTTAAGCCACGTCAACATTTTATATTGTTTTTTTATAAAAATAATAAAACAAAAAGAAATAGTAATATAAAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

253

Amino Acids

28.57

Weight (kDa)

7.68

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 33 - 201 5.9e-46 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04937 AT5G05030 AT5G11660 AT5G25410 AT5G25415 AT5G36680 AT5G36770 AT5G60380
fragaria_vesca FvH4_2g09090 FvH4_4g25910
malus_domestica MD10G1076000.v1.1 MD10G1207400.v1.1
pyrus_communis pycom02g18760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
Acc36I ACCTGC 1 cut(s) 597
AciI CCGC 2 cut(s) 140, 239
AcoI YGGCCR 2 cut(s) 437, 541
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 565
AfaI GTAC 3 cut(s) 282, 578, 647
AfiI CCNNNNNNNGG 2 cut(s) 533, 534
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 2 cut(s) 82, 183
AhlI ACTAGT 1 cut(s) 448
AjiI CACGTC 1 cut(s) 692
AjnI CCWGG 2 cut(s) 270, 372
AluBI AGCT 2 cut(s) 37, 111
AluI AGCT 2 cut(s) 37, 111
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 4 cut(s) 136, 437, 524, 541
ApaI GGGCCC 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 359
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
Asp700I GAANNNNTTC 1 cut(s) 450
AspS9I GGNCC 4 cut(s) 137, 302, 524, 525
AsuHPI GGTGA 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 1 cut(s) 302
BaeGI GKGCMC 1 cut(s) 528
BalI TGGCCA 2 cut(s) 439, 543
BanII GRGCYC 1 cut(s) 528
BbvI GCAGC 1 cut(s) 16
BccI CCATC 4 cut(s) 480, 515, 526, 548
BciT130I CCWGG 2 cut(s) 272, 374
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 3 cut(s) 108, 449, 456
BfuAI ACCTGC 1 cut(s) 597
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 2 cut(s) 272, 374
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 210
BmgBI CACGTC 1 cut(s) 692
BmgT120I GGNCC 4 cut(s) 137, 302, 524, 525
BmiI GGNNCC 4 cut(s) 172, 507, 526, 527
BmrFI CCNGG 2 cut(s) 272, 374
BmsI GCATC 3 cut(s) 226, 242, 343
BpmI CTGGAG 2 cut(s) 492, 495
BsaAI YACGTR 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 271, 440, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 534
Bse1I ACTGG 2 cut(s) 230, 323
BseBI CCWGG 2 cut(s) 272, 374
BseDI CCNNGG 3 cut(s) 271, 440, 501
BseGI GGATG 3 cut(s) 241, 257, 491
BseLI CCNNNNNNNGG 2 cut(s) 533, 534
BseNI ACTGG 2 cut(s) 230, 323
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 1 cut(s) 16
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 4 cut(s) 138, 439, 526, 543
BsiEI CGRYCG 1 cut(s) 292
BsiHKCI CYCGRG 1 cut(s) 210
BslI CCNNNNNNNGG 2 cut(s) 533, 534
BsnI GGCC 4 cut(s) 138, 439, 526, 543
BsoBI CYCGRG 1 cut(s) 210
Bsp120I GGGCCC 1 cut(s) 524
Bsp1286I GDGCHC 1 cut(s) 528
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 2 cut(s) 140, 239
BspANI GGCC 4 cut(s) 138, 439, 526, 543
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 4 cut(s) 172, 507, 526, 527
BspMI ACCTGC 1 cut(s) 597
BsrI ACTGG 2 cut(s) 230, 323
BssECI CCNNGG 3 cut(s) 271, 440, 501
BssT1I CCWWGG 2 cut(s) 440, 501
Bst2UI CCWGG 2 cut(s) 272, 374
Bst4CI ACNGT 1 cut(s) 682
BstBAI YACGTR 1 cut(s) 561
BstC8I GCNNGC 2 cut(s) 140, 158
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 3 cut(s) 241, 257, 491
BstMCI CGRYCG 1 cut(s) 292
BstNI CCWGG 2 cut(s) 272, 374
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 270, 372
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 16
BsuRI GGCC 4 cut(s) 138, 439, 526, 543
BtgI CCRYGG 1 cut(s) 501
BtrI CACGTC 1 cut(s) 692
BtsCI GGATG 3 cut(s) 241, 257, 491
BtsIMutI CAGTG 1 cut(s) 330
BveI ACCTGC 1 cut(s) 597
Cac8I GCNNGC 2 cut(s) 140, 158
CciI TCATGA 1 cut(s) 597
Cfr13I GGNCC 4 cut(s) 137, 302, 524, 525
Csp6I GTAC 3 cut(s) 281, 577, 646
CviAII CATG 5 cut(s) 91, 502, 569, 598, 611
CviQI GTAC 3 cut(s) 281, 577, 646
EaeI YGGCCR 2 cut(s) 437, 541
Eco130I CCWWGG 2 cut(s) 440, 501
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 565
Eco88I CYCGRG 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 302, 525
EcoRII CCWGG 2 cut(s) 270, 372
EcoT14I CCWWGG 2 cut(s) 440, 501
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 2 cut(s) 440, 501
FaeI CATG 5 cut(s) 94, 505, 572, 601, 614
FatI CATG 5 cut(s) 90, 501, 568, 597, 610
FauI CCCGC 1 cut(s) 147
FauNDI CATATG 1 cut(s) 349
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 3 cut(s) 248, 264, 498
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 3 cut(s) 108, 449, 456
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 2 cut(s) 492, 495
HaeIII GGCC 4 cut(s) 138, 439, 526, 543
Hin1II CATG 5 cut(s) 94, 505, 572, 601, 614
HincII GTYRAC 1 cut(s) 695
HindII GTYRAC 1 cut(s) 695
HinfI GANTC 2 cut(s) 260, 276
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 695
Hpy188I TCNGA 1 cut(s) 584
Hpy188III TCNNGA 4 cut(s) 64, 474, 509, 598
Hpy8I GTNNAC 1 cut(s) 695
HpyAV CCTTC 2 cut(s) 595, 608
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4IV ACGT 4 cut(s) 150, 297, 560, 691
HpyCH4V TGCA 3 cut(s) 4, 566, 592
HpySE526I ACGT 4 cut(s) 150, 297, 560, 691
Hsp92II CATG 5 cut(s) 94, 505, 572, 601, 614
LmnI GCTCC 2 cut(s) 341, 511
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 226, 242, 343
MaeI CTAG 3 cut(s) 108, 449, 456
MaeII ACGT 4 cut(s) 150, 297, 560, 691
MaeIII GTNAC 1 cut(s) 676
MboII GAAGA 2 cut(s) 530, 653
MhlI GDGCHC 1 cut(s) 528
MlsI TGGCCA 2 cut(s) 439, 543
MluCI AATT 4 cut(s) 42, 359, 391, 495
MluNI TGGCCA 2 cut(s) 439, 543
MnlI CCTC 3 cut(s) 219, 485, 644
Mox20I TGGCCA 2 cut(s) 439, 543
MroXI GAANNNNTTC 1 cut(s) 450
MscI TGGCCA 2 cut(s) 439, 543
MseI TTAA 2 cut(s) 684, 757
MslI CAYNNNNRTG 1 cut(s) 20
Msp20I TGGCCA 2 cut(s) 439, 543
MspA1I CMGCKG 1 cut(s) 37
MspR9I CCNGG 2 cut(s) 272, 374
MvaI CCWGG 2 cut(s) 272, 374
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 349
NlaIII CATG 5 cut(s) 94, 505, 572, 601, 614
NlaIV GGNNCC 4 cut(s) 172, 507, 526, 527
NmuCI GTSAC 1 cut(s) 676
NspI RCATGY 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 210
PagI TCATGA 1 cut(s) 597
PciI ACATGT 1 cut(s) 568
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 294
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 2 cut(s) 260, 276
PkrI GCNGC 1 cut(s) 6
Ppu21I YACGTR 1 cut(s) 561
PpuMI RGGWCCY 1 cut(s) 302
PscI ACATGT 1 cut(s) 568
PsiI TTATAA 1 cut(s) 717
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 270, 372
PspGI CCWGG 2 cut(s) 270, 372
PspN4I GGNNCC 4 cut(s) 172, 507, 526, 527
PspOMI GGGCCC 1 cut(s) 524
PspPI GGNCC 4 cut(s) 137, 302, 524, 525
PspPPI RGGWCCY 1 cut(s) 302
PspXI VCTCGAGB 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 37
RsaI GTAC 3 cut(s) 282, 578, 647
RsaNI GTAC 3 cut(s) 281, 577, 646
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 2 cut(s) 684, 757
SatI GCNGC 1 cut(s) 5
Sau96I GGNCC 4 cut(s) 137, 302, 524, 525
ScrFI CCNGG 2 cut(s) 272, 374
SduI GDGCHC 1 cut(s) 528
SetI ASST 8 cut(s) 39, 113, 153, 300, 304, 563, 591, 694
SfaNI GCATC 3 cut(s) 226, 242, 343
Sfr274I CTCGAG 1 cut(s) 210
SinI GGWCC 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
SpeI ACTAGT 1 cut(s) 448
Sse9I AATT 4 cut(s) 42, 359, 391, 495
SsiI CCGC 2 cut(s) 140, 239
SspI AATATT 1 cut(s) 755
SspMI CTAG 3 cut(s) 108, 449, 456
StyD4I CCNGG 2 cut(s) 270, 372
StyI CCWWGG 2 cut(s) 440, 501
TaaI ACNGT 1 cut(s) 682
TaiI ACGT 4 cut(s) 153, 300, 563, 694
TaqI TCGA 4 cut(s) 129, 211, 288, 363
TaqII GACCGA 1 cut(s) 306
TasI AATT 4 cut(s) 42, 359, 391, 495
TfiI GAWTC 2 cut(s) 260, 276
Tru1I TTAA 2 cut(s) 684, 757
Tru9I TTAA 2 cut(s) 684, 757
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 5 cut(s) 4, 38, 586, 614, 627
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 359
XceI RCATGY 1 cut(s) 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XhoI CTCGAG 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 3 cut(s) 108, 449, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.