AT5G25640

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012141
Physical Location & Seq
Reverse (-)
8949515 .. 8951173
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25640.1

Sequence Viewer

Length: 864 bp
ATGTCTCAGCTTCACCTTCACCTTCAGCTTCACCGTCACTATCTCTCCCTCCCACATTCTTCTTCTCTTCGTTCCAGATTACCGTCATTACATCGCCGCAGTGTAGCTTCTTCGCCAACCAACTCTCCCCTCTATCTCAACTCAACTAACTCCACCGTCTTTCGTCCTCTCACCTTCGTCAGATCTCGTTCCAGCGAATCCGACGTCACACCACAATTTGAATTGGATAAAGCACAAGACAAACAGAAACCACTAAACCAAGCAAATGGAATATTCTTGATCATACTTATCAATCTTGCCATATTTATAGCTCAACACTTCTATCAGGTTCGCGGGATCAAATCGATGTATTTGTCTAGCGATTTTCCGGTATGGTACCAGTTTGTGACTGCAACATTCTGTCATGCTAACTGGAACCATCTGTCCAGCAATCTTTTTCTCTTGTACATCTTTGGAAAGATTGTTGAAGAAGAGAAAGGAAACTTTGGTTTGTGGTTGTCTTACTTGTTTACTGGTGTTGGAGCTAACATGGTTTCTTGGTTCACTTTACCGCCAAATTCATTTTTTTCTGGAGCCTCGGGAGCTGTTTTCGGGTTATTTGTGATCAGTGTCCTTGTCAAGATGTCTCGGGGTTGGAGCAAATTTCTTGAAGTTCTTATATTGGGTCAGTTTGTTATGAAGAGGGTATCTGAAGCAGTTCAGACTTCAACTGGATTATTAGAAACTAAATTTTGTGGATTTATATTTGGTGGCAATGAATTCCTGACAATTATTCAGACTGTTAGACCCGTTGCGCAGTTCTCGGGTGCTCTTCTTGGTGTTCTCTTAGTTTGGCTTCTCAGCAAATTTCCCTTAGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

287

Amino Acids

32.41

Weight (kDa)

9.73

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 125 - 282 2.6e-19 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25640
fragaria_vesca FvH4_2g36120 FvH4_2g36120 FvH4_2g36120 FvH4_2g36120
malus_domestica MD15G1097700.v1.1
prunus_persica Prupe.1G452600_v2.0.a1
pyrus_communis pycom08g09880 pycom15g08960
rosa_chinensis RchiOBHm_Chr6g0305751
rosa_laevigata RLG00000010862
rosa_multiflora Rmu_sc0042960.1_g000002
rosa_roxburghii Rroxscaffold_7G00162380
rosa_rugosa Rorug06G0343900.1
rosa_samantha Rh6AG455300 Rh6BG446000 Rh6CG469200 Rh6DG455800
rosa_wichuraiana Rw6G039720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 207
Acc16I TGCGCA 1 cut(s) 795
Acc65I GGTACC 1 cut(s) 375
AccB1I GGYRCC 1 cut(s) 375
AccII CGCG 1 cut(s) 333
AciI CCGC 3 cut(s) 97, 333, 551
AclWI GGATC 1 cut(s) 344
AcsI RAATTY 5 cut(s) 556, 641, 728, 758, 845
AcuI CTGAAG 2 cut(s) 8, 711
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 377, 446
AgsI TTSAA 4 cut(s) 221, 467, 650, 708
AloI GAACNNNNNNTCC 2 cut(s) 407, 439
AluBI AGCT 6 cut(s) 10, 28, 107, 311, 524, 584
AluI AGCT 6 cut(s) 10, 28, 107, 311, 524, 584
Alw21I GWGCWC 1 cut(s) 811
Alw26I GTCTC 2 cut(s) 9, 630
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 3 cut(s) 577, 627, 802
ApoI RAATTY 5 cut(s) 556, 641, 728, 758, 845
ArsI GACNNNNNNTTYG 2 cut(s) 189, 221
Asp700I GAANNNNTTC 1 cut(s) 696
Asp718I GGTACC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 796
AsuHPI GGTGA 4 cut(s) 5, 11, 23, 163
AvaI CYCGRG 3 cut(s) 577, 627, 802
BanI GGYRCC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 811
BccI CCATC 1 cut(s) 426
BclI TGATCA 2 cut(s) 279, 603
BcoDI GTCTC 2 cut(s) 9, 630
BfaI CTAG 1 cut(s) 357
BglII AGATCT 1 cut(s) 182
BisI GCNGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 98
BmeT110I CYCGRG 3 cut(s) 577, 627, 802
BmiI GGNNCC 3 cut(s) 377, 416, 574
BpmI CTGGAG 1 cut(s) 591
Bsa29I ATCGAT 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 576
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 4 cut(s) 29, 59, 109, 139
Bse1I ACTGG 4 cut(s) 379, 416, 517, 715
Bse3DI GCAATG 1 cut(s) 760
BseCI ATCGAT 1 cut(s) 344
BseDI CCNNGG 1 cut(s) 576
BseMI GCAATG 1 cut(s) 760
BseMII CTCAG 2 cut(s) 20, 853
BseNI ACTGG 4 cut(s) 379, 416, 517, 715
Bsh1236I CGCG 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 375
BshVI ATCGAT 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 811
BsiHKCI CYCGRG 3 cut(s) 577, 627, 802
BsiSI CCGG 1 cut(s) 368
BsmAI GTCTC 2 cut(s) 9, 630
BsoBI CYCGRG 3 cut(s) 577, 627, 802
Bsp1286I GDGCHC 1 cut(s) 811
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 4 cut(s) 182, 279, 336, 603
BspACI CCGC 3 cut(s) 97, 333, 551
BspCNI CTCAG 2 cut(s) 19, 852
BspDI ATCGAT 1 cut(s) 344
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 3 cut(s) 377, 416, 574
BspPI GGATC 1 cut(s) 344
BspQI GCTCTTC 1 cut(s) 816
BspT107I GGYRCC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 760
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 4 cut(s) 379, 416, 517, 715
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 4 cut(s) 182, 279, 336, 603
BssNI GRCGYC 1 cut(s) 204
Bst4CI ACNGT 4 cut(s) 35, 84, 157, 781
Bst6I CTCTTC 4 cut(s) 72, 465, 674, 816
BstACI GRCGYC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 444
BstDEI CTNAG 4 cut(s) 6, 826, 839, 853
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 796
BstKTI GATC 4 cut(s) 185, 282, 339, 606
BstMAI GTCTC 2 cut(s) 9, 630
BstMBI GATC 4 cut(s) 182, 279, 336, 603
BstMWI GCNNNNNNNGC 1 cut(s) 581
BstUI CGCG 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 182
BstXI CCANNNNNNTGG 1 cut(s) 266
BstYI RGATCY 1 cut(s) 182
Bsu15I ATCGAT 1 cut(s) 344
BsuTUI ATCGAT 1 cut(s) 344
BtgZI GCGATG 1 cut(s) 77
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 106, 613
CfoI GCGC 1 cut(s) 796
ClaI ATCGAT 1 cut(s) 344
Csp6I GTAC 2 cut(s) 376, 445
CviAII CATG 2 cut(s) 404, 529
CviJI RGCY 8 cut(s) 10, 28, 107, 311, 524, 575, 584, 835
CviKI_1 RGCY 8 cut(s) 10, 28, 107, 311, 524, 575, 584, 835
CviQI GTAC 2 cut(s) 376, 445
DdeI CTNAG 4 cut(s) 6, 826, 839, 853
DpnI GATC 4 cut(s) 184, 281, 338, 605
DpnII GATC 4 cut(s) 182, 279, 336, 603
Eam1104I CTCTTC 4 cut(s) 72, 465, 674, 816
EarI CTCTTC 4 cut(s) 72, 465, 674, 816
Eco57I CTGAAG 2 cut(s) 8, 711
Eco88I CYCGRG 3 cut(s) 577, 627, 802
EcoRI GAATTC 1 cut(s) 758
FaeI CATG 2 cut(s) 407, 532
FaiI YATR 9 cut(s) 284, 302, 308, 373, 405, 530, 659, 677, 743
FatI CATG 2 cut(s) 403, 528
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 2 cut(s) 279, 603
Fnu4HI GCNGC 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 97
FspBI CTAG 1 cut(s) 357
FspI TGCGCA 1 cut(s) 795
GlaI GCGC 1 cut(s) 795
GluI GCNGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 591
HapII CCGG 1 cut(s) 368
HhaI GCGC 1 cut(s) 796
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 407, 532
Hin6I GCGC 1 cut(s) 794
HinP1I GCGC 1 cut(s) 794
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 368
HphI GGTGA 4 cut(s) 5, 11, 23, 163
Hpy166II GTNNAC 2 cut(s) 510, 543
Hpy188I TCNGA 5 cut(s) 182, 202, 691, 702, 777
Hpy188III TCNNGA 7 cut(s) 75, 277, 570, 579, 619, 647, 763
Hpy8I GTNNAC 2 cut(s) 510, 543
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 3 cut(s) 26, 32, 184
HpyCH4III ACNGT 4 cut(s) 35, 84, 157, 781
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 1 cut(s) 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 581
HpyF3I CTNAG 4 cut(s) 6, 826, 839, 853
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 407, 532
HspAI GCGC 1 cut(s) 794
KpnI GGTACC 1 cut(s) 379
Ksp22I TGATCA 2 cut(s) 279, 603
Kzo9I GATC 4 cut(s) 182, 279, 336, 603
LguI GCTCTTC 1 cut(s) 816
LmnI GCTCC 4 cut(s) 521, 572, 581, 636
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 3 cut(s) 35, 205, 385
MalI GATC 4 cut(s) 184, 281, 338, 605
MboI GATC 4 cut(s) 182, 279, 336, 603
MboII GAAGA 8 cut(s) 51, 54, 59, 102, 479, 482, 691, 803
MflI RGATCY 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 811
MluCI AATT 8 cut(s) 215, 221, 556, 641, 728, 758, 768, 845
MmeI TCCRAC 3 cut(s) 225, 499, 614
MnlI CCTC 5 cut(s) 59, 140, 177, 586, 675
MroXI GAANNNNTTC 1 cut(s) 696
MspI CCGG 1 cut(s) 368
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 581
NdeII GATC 4 cut(s) 182, 279, 336, 603
NlaIII CATG 2 cut(s) 407, 532
NlaIV GGNNCC 3 cut(s) 377, 416, 574
NmuCI GTSAC 3 cut(s) 35, 205, 385
NsbI TGCGCA 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 377, 416, 574
PsuI RGATCY 1 cut(s) 182
RsaI GTAC 2 cut(s) 377, 446
RsaNI GTAC 2 cut(s) 376, 445
SapI GCTCTTC 1 cut(s) 816
SatI GCNGC 1 cut(s) 97
Sau3AI GATC 4 cut(s) 182, 279, 336, 603
SduI GDGCHC 1 cut(s) 811
Sse9I AATT 8 cut(s) 215, 221, 556, 641, 728, 758, 768, 845
SsiI CCGC 3 cut(s) 97, 333, 551
SspI AATATT 1 cut(s) 273
SspMI CTAG 1 cut(s) 357
TaaI ACNGT 4 cut(s) 35, 84, 157, 781
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 344
TasI AATT 8 cut(s) 215, 221, 556, 641, 728, 758, 768, 845
TatI WGTACW 1 cut(s) 444
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 1 cut(s) 197
TscAI CASTG 2 cut(s) 106, 613
TseFI GTSAC 3 cut(s) 35, 205, 385
Tsp45I GTSAC 3 cut(s) 35, 205, 385
TspDTI ATGAA 3 cut(s) 549, 692, 771
TspRI CASTG 2 cut(s) 106, 613
XapI RAATTY 5 cut(s) 556, 641, 728, 758, 845
XmnI GAANNNNTTC 1 cut(s) 696
XspI CTAG 1 cut(s) 357
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.