MD15G1097700.v1.1

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012141
Physical Location & Seq
Forward (+)
6805667 .. 6807910
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1097700.v1.1.491

Sequence Viewer

Length: 906 bp
ATGTCCCGGCTTCTGCTCCAGCTCCAGCATCTTCAAACGCTCACTAAACCCAGCAAACTCCAATTCGTACCCAGCACTTGCTTCTCTCACCATCCCAACCTCACTCTCAATCTCAAGTTTCCCCCCGACCACACCAAACCCAGCAAACTCCACCTCTCTCTCCGGCCCCTTCTTCCCGCCACCGCTCGATCCCCCATAATAGTCTGCAAATCCAACGGCTCAGACAGCCTAGACTTAGATGTTCTATCACGGCCAGAAGAGCGAGGGAAGCCGCACCGACGCGCCAACGGAATATTCTGGATTATACTTGCTAACATTGGGATTTACTTAGCTGATCATGTGTTCCAGGTTCGCGCGATTAAAATGCTCTACTTGTACCACAATTGGCCTACATGGTACCAGTTTCTAACAGCAACATTCTGTCATGCTAATTGGAGCCATCTCTCGAGCAACCTCTTCTTCTTGTATATTTTTGGTAAGCTAGTTGAAGAAGAGGAAGGGAATTTTGCTCTGTGGCTTTGTTATATTCTTACCGGGGTTGGAGCAAACCTTGTATCGTGGCTTGTTCTACCACGAAATGCAGTTTCAGTTGGAGCCTCTGGTGCTGTTTTCGGATTATTTACCATTAGTGTGCTTGTAAAGATGTCTTGGGACTGGAGGAAGATCCTCGAAGTGCTTATATTGGGCCAATTTGTTGTAGAAAAGGTTATGGAAGCAGCCCAAGCTTCTACTGCATTATCGGGCACCTTTGGTAGAAATTCGTTGATGAATGTCAATCACATTGCCCATCTCTCAGGTGCTCTTGTTGGTGTTTTTCTGGTATGGATTCTCAGCAAGGTTCCTTCTGAACCCCCATCACAAGAAGGATCGAATTTCCAAAGCAAAGCAGGCAGAACTCGGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

302

Amino Acids

33.74

Weight (kDa)

9.61

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 131 - 279 7.1e-28 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25640
fragaria_vesca FvH4_2g36120 FvH4_2g36120 FvH4_2g36120 FvH4_2g36120
malus_domestica MD15G1097700.v1.1
prunus_persica Prupe.1G452600_v2.0.a1
pyrus_communis pycom08g09880 pycom15g08960
rosa_chinensis RchiOBHm_Chr6g0305751
rosa_laevigata RLG00000010862
rosa_multiflora Rmu_sc0042960.1_g000002
rosa_roxburghii Rroxscaffold_7G00162380
rosa_rugosa Rorug06G0343900.1
rosa_samantha Rh6AG455300 Rh6BG446000 Rh6CG469200 Rh6DG455800
rosa_wichuraiana Rw6G039720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 904
Acc65I GGTACC 1 cut(s) 396
AccB1I GGYRCC 2 cut(s) 396, 743
AccBSI CCGCTC 1 cut(s) 185
AccII CGCG 3 cut(s) 282, 354, 356
AciI CCGC 3 cut(s) 177, 183, 272
AclWI GGATC 3 cut(s) 183, 658, 874
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 3 cut(s) 502, 757, 871
AfaI GTAC 3 cut(s) 69, 377, 398
AgsI TTSAA 2 cut(s) 35, 488
AjnI CCWGG 1 cut(s) 345
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 4 cut(s) 22, 332, 481, 725
AluI AGCT 4 cut(s) 22, 332, 481, 725
Alw21I GWGCWC 1 cut(s) 802
AlwI GGATC 3 cut(s) 183, 658, 874
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 4 cut(s) 164, 251, 386, 685
ApeKI GCWGC 1 cut(s) 716
ApoI RAATTY 3 cut(s) 502, 757, 871
Asp718I GGTACC 1 cut(s) 396
AspLEI GCGC 2 cut(s) 284, 356
AspS9I GGNCC 2 cut(s) 165, 685
AsuC2I CCSGG 2 cut(s) 7, 535
AsuHPI GGTGA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 746
BanI GGYRCC 2 cut(s) 396, 743
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 1 cut(s) 728
BccI CCATC 4 cut(s) 99, 447, 795, 862
BceAI ACGGC 2 cut(s) 232, 266
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 1 cut(s) 347
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 2 cut(s) 7, 535
BfaI CTAG 2 cut(s) 230, 482
BisI GCNGC 2 cut(s) 272, 717
BlsI GCNGC 2 cut(s) 273, 718
Bme1390I CCNGG 3 cut(s) 7, 347, 535
BmeT110I CYCGRG 1 cut(s) 445
BmgT120I GGNCC 2 cut(s) 165, 685
BmiI GGNNCC 6 cut(s) 167, 398, 437, 595, 745, 840
BmrFI CCNGG 3 cut(s) 7, 347, 535
BmsI GCATC 1 cut(s) 37
BpmI CTGGAG 2 cut(s) 8, 676
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 2 cut(s) 7, 535
BsaJI CCNNGG 1 cut(s) 534
Bse1I ACTGG 2 cut(s) 400, 659
Bse3DI GCAATG 1 cut(s) 780
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 534
BseGI GGATG 1 cut(s) 91
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 3 cut(s) 234, 807, 844
BseNI ACTGG 2 cut(s) 400, 659
BseSI GKGCMC 1 cut(s) 746
BseXI GCAGC 1 cut(s) 728
BseYI CCCAGC 3 cut(s) 50, 71, 140
Bsh1236I CGCG 3 cut(s) 282, 354, 356
BshFI GGCC 4 cut(s) 166, 253, 388, 687
BshNI GGYRCC 2 cut(s) 396, 743
BsiHKAI GWGCWC 1 cut(s) 802
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 3 cut(s) 7, 163, 534
BslFI GGGAC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 4 cut(s) 166, 253, 388, 687
BsoBI CYCGRG 1 cut(s) 445
Bsp1286I GDGCHC 2 cut(s) 746, 802
Bsp143I GATC 4 cut(s) 188, 334, 663, 866
BspACI CCGC 3 cut(s) 177, 183, 272
BspANI GGCC 4 cut(s) 166, 253, 388, 687
BspCNI CTCAG 3 cut(s) 233, 806, 843
BspFNI CGCG 3 cut(s) 282, 354, 356
BspLI GGNNCC 6 cut(s) 167, 398, 437, 595, 745, 840
BspPI GGATC 3 cut(s) 183, 658, 874
BspQI GCTCTTC 1 cut(s) 252
BspT107I GGYRCC 2 cut(s) 396, 743
BsrBI CCGCTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 780
BsrI ACTGG 2 cut(s) 400, 659
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 4 cut(s) 188, 334, 663, 866
Bst2UI CCWGG 1 cut(s) 347
Bst6I CTCTTC 3 cut(s) 252, 461, 486
BstC8I GCNNGC 1 cut(s) 889
BstDEI CTNAG 5 cut(s) 220, 235, 328, 793, 830
BstF5I GGATG 1 cut(s) 91
BstFNI CGCG 3 cut(s) 282, 354, 356
BstHHI GCGC 2 cut(s) 284, 356
BstKTI GATC 4 cut(s) 191, 337, 666, 869
BstMBI GATC 4 cut(s) 188, 334, 663, 866
BstMWI GCNNNNNNNGC 7 cut(s) 225, 259, 268, 602, 722, 731, 888
BstNI CCWGG 1 cut(s) 347
BstSCI CCNGG 3 cut(s) 5, 345, 533
BstSLI GKGCMC 1 cut(s) 746
BstUI CGCG 3 cut(s) 282, 354, 356
BstV1I GCAGC 1 cut(s) 728
BstX2I RGATCY 1 cut(s) 663
BstYI RGATCY 1 cut(s) 663
BsuRI GGCC 4 cut(s) 166, 253, 388, 687
BtsCI GGATG 1 cut(s) 91
Cac8I GCNNGC 1 cut(s) 889
CfoI GCGC 2 cut(s) 284, 356
Cfr13I GGNCC 2 cut(s) 165, 685
CseI GACGC 1 cut(s) 288
Csp6I GTAC 3 cut(s) 68, 376, 397
CviAII CATG 3 cut(s) 338, 393, 425
CviQI GTAC 3 cut(s) 68, 376, 397
DdeI CTNAG 5 cut(s) 220, 235, 328, 793, 830
DpnI GATC 4 cut(s) 190, 336, 665, 868
DpnII GATC 4 cut(s) 188, 334, 663, 866
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 3 cut(s) 252, 461, 486
EarI CTCTTC 3 cut(s) 252, 461, 486
Eco88I CYCGRG 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 345
FaeI CATG 3 cut(s) 341, 396, 428
FaqI GGGAC 1 cut(s) 665
FatI CATG 3 cut(s) 337, 392, 424
FauI CCCGC 1 cut(s) 184
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 272, 717
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 2 cut(s) 272, 717
FspBI CTAG 2 cut(s) 230, 482
GlaI GCGC 2 cut(s) 283, 355
GluI GCNGC 2 cut(s) 272, 717
GsaI CCCAGC 3 cut(s) 54, 75, 144
GsuI CTGGAG 2 cut(s) 8, 676
HaeIII GGCC 4 cut(s) 166, 253, 388, 687
HapII CCGG 3 cut(s) 7, 163, 534
HgaI GACGC 1 cut(s) 288
HhaI GCGC 2 cut(s) 284, 356
Hin1II CATG 3 cut(s) 341, 396, 428
Hin6I GCGC 2 cut(s) 282, 354
HinP1I GCGC 2 cut(s) 282, 354
HindIII AAGCTT 1 cut(s) 723
HinfI GANTC 1 cut(s) 826
HpaII CCGG 3 cut(s) 7, 163, 534
HphI GGTGA 1 cut(s) 80
Hpy188I TCNGA 3 cut(s) 223, 614, 847
Hpy188III TCNNGA 2 cut(s) 298, 445
Hpy99I CGWCG 1 cut(s) 282
HpyAV CCTTC 4 cut(s) 179, 491, 852, 857
HpyCH4V TGCA 3 cut(s) 207, 581, 734
HpyF10VI GCNNNNNNNGC 7 cut(s) 225, 259, 268, 602, 722, 731, 888
HpyF3I CTNAG 5 cut(s) 220, 235, 328, 793, 830
Hsp92II CATG 3 cut(s) 341, 396, 428
HspAI GCGC 2 cut(s) 282, 354
KpnI GGTACC 1 cut(s) 400
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 4 cut(s) 188, 334, 663, 866
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 5 cut(s) 21, 27, 435, 542, 593
Lsp1109I GCAGC 1 cut(s) 728
LweI GCATC 1 cut(s) 37
MaeI CTAG 2 cut(s) 230, 482
MalI GATC 4 cut(s) 190, 336, 665, 868
MbiI CCGCTC 1 cut(s) 185
MboI GATC 4 cut(s) 188, 334, 663, 866
MboII GAAGA 8 cut(s) 23, 164, 269, 448, 451, 500, 503, 673
MfeI CAATTG 1 cut(s) 382
MflI RGATCY 1 cut(s) 663
MhlI GDGCHC 2 cut(s) 746, 802
MluCI AATT 7 cut(s) 62, 382, 430, 502, 689, 757, 871
MmeI TCCRAC 3 cut(s) 237, 520, 571
MnlI CCTC 8 cut(s) 110, 164, 257, 464, 487, 607, 651, 677
MseI TTAA 1 cut(s) 360
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 3 cut(s) 7, 163, 534
MspR9I CCNGG 3 cut(s) 7, 347, 535
MunI CAATTG 1 cut(s) 382
MvaI CCWGG 1 cut(s) 347
MvnI CGCG 3 cut(s) 282, 354, 356
MwoI GCNNNNNNNGC 7 cut(s) 225, 259, 268, 602, 722, 731, 888
NciI CCSGG 2 cut(s) 7, 535
NdeII GATC 4 cut(s) 188, 334, 663, 866
NlaIII CATG 3 cut(s) 341, 396, 428
NlaIV GGNNCC 6 cut(s) 167, 398, 437, 595, 745, 840
PaeR7I CTCGAG 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 252
PfeI GAWTC 1 cut(s) 826
PkrI GCNGC 2 cut(s) 273, 718
PsiI TTATAA 1 cut(s) 904
Psp6I CCWGG 1 cut(s) 345
PspFI CCCAGC 3 cut(s) 50, 71, 140
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 6 cut(s) 167, 398, 437, 595, 745, 840
PspPI GGNCC 2 cut(s) 165, 685
PsuI RGATCY 1 cut(s) 663
RsaI GTAC 3 cut(s) 69, 377, 398
RsaNI GTAC 3 cut(s) 68, 376, 397
RseI CAYNNNNRTG 1 cut(s) 629
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 360
SatI GCNGC 2 cut(s) 272, 717
Sau3AI GATC 4 cut(s) 188, 334, 663, 866
Sau96I GGNCC 2 cut(s) 165, 685
ScrFI CCNGG 3 cut(s) 7, 347, 535
SduI GDGCHC 2 cut(s) 746, 802
SfaNI GCATC 1 cut(s) 37
Sfr274I CTCGAG 1 cut(s) 445
SlaI CTCGAG 1 cut(s) 445
SmiMI CAYNNNNRTG 1 cut(s) 629
SmlI CTYRAG 2 cut(s) 113, 445
SmoI CTYRAG 2 cut(s) 113, 445
Sse9I AATT 7 cut(s) 62, 382, 430, 502, 689, 757, 871
SsiI CCGC 3 cut(s) 177, 183, 272
SspI AATATT 1 cut(s) 294
SspMI CTAG 2 cut(s) 230, 482
StyD4I CCNGG 3 cut(s) 5, 345, 533
TaqI TCGA 4 cut(s) 187, 446, 669, 869
TasI AATT 7 cut(s) 62, 382, 430, 502, 689, 757, 871
TauI GCSGC 1 cut(s) 274
TfiI GAWTC 1 cut(s) 826
Tru1I TTAA 1 cut(s) 360
Tru9I TTAA 1 cut(s) 360
TseI GCWGC 1 cut(s) 716
TspDTI ATGAA 1 cut(s) 782
TspGWI ACGGA 1 cut(s) 303
XapI RAATTY 3 cut(s) 502, 757, 871
XhoI CTCGAG 1 cut(s) 445
XspI CTAG 2 cut(s) 230, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.