RLG00000010862

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012141
Physical Location & Seq
Forward (+)
4856308 .. 4857924
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010862

Sequence Viewer

Length: 849 bp
ATGTTCAAACCCTCACTAAACCTCACTACTCTTACTCATCCCACCATCCAGTCCCAATCACTTGCTTCCCTTATCGCCCCAACCTCACTCTCACCTTCCCTCATTCCCTCTCTACTTCCCAAAACCACCCCCAAAGCCCTCCACCTCCCTCTCCGCCCTCTCCTCACCGCCGTCCGATCTCGCATAGTCTGCAAATCGAGCGGCTCAGAATTTGATATCCTATCGCAGCCAGAAGAGAGAAGAAGACCTCACAGACGCGCCAATGGAATATTCTGGATTTTACTTATTAATATCGGAATATACTTGGCCGATCATATTTTTCAGGTTCCCGCAGTTAAAATGCTATACTTGTATCACAGTTACCCTGAATGGTACCAGTTTCTAACAGCAACGTTCTGTCATGCTAATTGGAACCATCTTTCGAGCAACCTTTTCTTCTTGTATATTTTCGGAAAGCTAGTTGAAGAAGATGAAGGAAATCTCGCACTGTGGCTCTCTTATATTCTTACAGGTGTTGGAGCTAACTTTGTTTCGTGGCTTGCTCTACCAACAAATGCAGTTTCTATTGGAGCTTCTGGTGCTGTTTTTGGATTGTTTACTATCAGTGTTCTTGTAAAGATGTCCTGGGACTGGAGGAAGATCCTTGAAGTTCTTATATTGGGCCAATTTGTTATAGACAAGGTTATGGAAGCAGCCCAAGCTTCGACAGGATTAGCAGGCCGCGTAGGCTCCTCTTTGATGAATGTCAATCACATTGCGCATCTCTCAGGTGCTCTTGTTGGTGTATTTCTAGTATGGCTTCTTGGTAGAATTCCTCTGAACCTCCAGAACAAGAAGGATTGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

283

Amino Acids

31.43

Weight (kDa)

9.51

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 123 - 270 3.6e-27 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25640
fragaria_vesca FvH4_2g36120 FvH4_2g36120 FvH4_2g36120 FvH4_2g36120
malus_domestica MD15G1097700.v1.1
prunus_persica Prupe.1G452600_v2.0.a1
pyrus_communis pycom08g09880 pycom15g08960
rosa_chinensis RchiOBHm_Chr6g0305751
rosa_laevigata RLG00000010862
rosa_multiflora Rmu_sc0042960.1_g000002
rosa_roxburghii Rroxscaffold_7G00162380
rosa_rugosa Rorug06G0343900.1
rosa_samantha Rh6AG455300 Rh6BG446000 Rh6CG469200 Rh6DG455800
rosa_wichuraiana Rw6G039720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 759
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 372
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 2 cut(s) 258, 723
AciI CCGC 5 cut(s) 154, 168, 201, 330, 721
AclI AACGTT 1 cut(s) 392
AclWI GGATC 1 cut(s) 634
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 2 cut(s) 209, 810
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 630
AgsI TTSAA 3 cut(s) 7, 464, 647
AjnI CCWGG 1 cut(s) 623
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 4 cut(s) 457, 521, 572, 701
AluI AGCT 4 cut(s) 457, 521, 572, 701
Alw21I GWGCWC 1 cut(s) 775
AlwI GGATC 1 cut(s) 634
AoxI GGCC 3 cut(s) 306, 661, 718
ApeKI GCWGC 2 cut(s) 226, 692
ApoI RAATTY 2 cut(s) 209, 810
AseI ATTAAT 1 cut(s) 288
Asp718I GGTACC 1 cut(s) 372
AspLEI GCGC 2 cut(s) 260, 760
AspS9I GGNCC 1 cut(s) 661
AsuHPI GGTGA 2 cut(s) 84, 157
BanI GGYRCC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 2 cut(s) 238, 704
BccI CCATC 2 cut(s) 53, 423
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 625
BfaI CTAG 2 cut(s) 458, 791
BglI GCCNNNNNGGC 1 cut(s) 726
BisI GCNGC 4 cut(s) 202, 227, 693, 721
BlsI GCNGC 4 cut(s) 203, 228, 694, 722
Bme1390I CCNGG 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 661
BmiI GGNNCC 4 cut(s) 327, 374, 413, 730
BmrFI CCNGG 1 cut(s) 625
BmsI GCATC 1 cut(s) 769
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 2 cut(s) 652, 809
BsaJI CCNNGG 1 cut(s) 624
Bsc4I CCNNNNNNNGG 1 cut(s) 630
Bse1I ACTGG 3 cut(s) 49, 376, 635
Bse3DI GCAATG 1 cut(s) 753
BseBI CCWGG 1 cut(s) 625
BseDI CCNNGG 1 cut(s) 624
BseGI GGATG 2 cut(s) 37, 45
BseLI CCNNNNNNNGG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 2 cut(s) 219, 780
BseNI ACTGG 3 cut(s) 49, 376, 635
BseRI GAGGAG 2 cut(s) 152, 721
BseXI GCAGC 2 cut(s) 238, 704
Bsh1236I CGCG 2 cut(s) 258, 723
BshFI GGCC 3 cut(s) 308, 663, 720
BshNI GGYRCC 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 775
BslFI GGGAC 2 cut(s) 37, 641
BslI CCNNNNNNNGG 1 cut(s) 630
BsmFI GGGAC 2 cut(s) 37, 641
BsnI GGCC 3 cut(s) 308, 663, 720
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 3 cut(s) 176, 310, 639
BspACI CCGC 5 cut(s) 154, 168, 201, 330, 721
BspANI GGCC 3 cut(s) 308, 663, 720
BspCNI CTCAG 2 cut(s) 218, 779
BspFNI CGCG 2 cut(s) 258, 723
BspLI GGNNCC 4 cut(s) 327, 374, 413, 730
BspPI GGATC 1 cut(s) 634
BspT107I GGYRCC 1 cut(s) 372
BsrBI CCGCTC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 753
BsrI ACTGG 3 cut(s) 49, 376, 635
BssECI CCNNGG 1 cut(s) 624
BssMI GATC 3 cut(s) 176, 310, 639
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 2 cut(s) 359, 489
Bst6I CTCTTC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 540, 718
BstDEI CTNAG 2 cut(s) 205, 766
BstF5I GGATG 2 cut(s) 37, 45
BstFNI CGCG 2 cut(s) 258, 723
BstHHI GCGC 2 cut(s) 260, 760
BstKTI GATC 3 cut(s) 179, 313, 642
BstMBI GATC 3 cut(s) 176, 310, 639
BstMWI GCNNNNNNNGC 5 cut(s) 189, 198, 578, 698, 726
BstNI CCWGG 1 cut(s) 625
BstSCI CCNGG 1 cut(s) 623
BstUI CGCG 2 cut(s) 258, 723
BstV1I GCAGC 2 cut(s) 238, 704
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 639
BstYI RGATCY 1 cut(s) 639
BsuRI GGCC 3 cut(s) 308, 663, 720
BtsCI GGATG 2 cut(s) 37, 45
BtsIMutI CAGTG 2 cut(s) 485, 610
Cac8I GCNNGC 2 cut(s) 540, 718
CfoI GCGC 2 cut(s) 260, 760
Cfr13I GGNCC 1 cut(s) 661
CseI GACGC 1 cut(s) 264
Csp6I GTAC 1 cut(s) 373
CviAII CATG 1 cut(s) 401
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 2 cut(s) 205, 766
DpnI GATC 3 cut(s) 178, 312, 641
DpnII GATC 3 cut(s) 176, 310, 639
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
EciI GGCGGA 1 cut(s) 143
Eco32I GATATC 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 810
EcoRII CCWGG 1 cut(s) 623
EcoRV GATATC 1 cut(s) 217
FaeI CATG 1 cut(s) 404
FaqI GGGAC 2 cut(s) 37, 641
FatI CATG 1 cut(s) 400
FauI CCCGC 1 cut(s) 337
Fnu4HI GCNGC 4 cut(s) 202, 227, 693, 721
FokI GGATG 2 cut(s) 24, 32
Fsp4HI GCNGC 4 cut(s) 202, 227, 693, 721
FspBI CTAG 2 cut(s) 458, 791
FspI TGCGCA 1 cut(s) 759
GlaI GCGC 2 cut(s) 259, 759
GluI GCNGC 4 cut(s) 202, 227, 693, 721
GsuI CTGGAG 2 cut(s) 652, 809
HaeIII GGCC 3 cut(s) 308, 663, 720
HgaI GACGC 1 cut(s) 264
HhaI GCGC 2 cut(s) 260, 760
Hin1II CATG 1 cut(s) 404
Hin6I GCGC 2 cut(s) 258, 758
HinP1I GCGC 2 cut(s) 258, 758
HindIII AAGCTT 1 cut(s) 699
HphI GGTGA 2 cut(s) 84, 157
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 5 cut(s) 176, 208, 296, 452, 819
Hpy188III TCNNGA 2 cut(s) 274, 826
Hpy8I GTNNAC 1 cut(s) 597
HpyAV CCTTC 3 cut(s) 105, 467, 829
HpyCH4III ACNGT 2 cut(s) 359, 489
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 2 cut(s) 192, 557
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 198, 578, 698, 726
HpyF3I CTNAG 2 cut(s) 205, 766
HpySE526I ACGT 1 cut(s) 392
Hsp92II CATG 1 cut(s) 404
HspAI GCGC 2 cut(s) 258, 758
KpnI GGTACC 1 cut(s) 376
Kzo9I GATC 3 cut(s) 176, 310, 639
LmnI GCTCC 3 cut(s) 518, 569, 734
Lsp1109I GCAGC 2 cut(s) 238, 704
LweI GCATC 1 cut(s) 769
MaeI CTAG 2 cut(s) 458, 791
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 1 cut(s) 359
MalI GATC 3 cut(s) 178, 312, 641
MbiI CCGCTC 1 cut(s) 201
MboI GATC 3 cut(s) 176, 310, 639
MboII GAAGA 7 cut(s) 245, 252, 255, 427, 476, 479, 649
MflI RGATCY 1 cut(s) 639
MhlI GDGCHC 1 cut(s) 775
MluCI AATT 4 cut(s) 209, 406, 665, 810
MmeI TCCRAC 1 cut(s) 496
MseI TTAA 2 cut(s) 288, 336
MspR9I CCNGG 1 cut(s) 625
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 2 cut(s) 258, 723
MwoI GCNNNNNNNGC 5 cut(s) 189, 198, 578, 698, 726
NdeII GATC 3 cut(s) 176, 310, 639
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 4 cut(s) 327, 374, 413, 730
NsbI TGCGCA 1 cut(s) 759
PkrI GCNGC 4 cut(s) 203, 228, 694, 722
PshBI ATTAAT 1 cut(s) 288
Psp1406I AACGTT 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 4 cut(s) 327, 374, 413, 730
PspPI GGNCC 1 cut(s) 661
PsuI RGATCY 1 cut(s) 639
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SaqAI TTAA 2 cut(s) 288, 336
SatI GCNGC 4 cut(s) 202, 227, 693, 721
Sau3AI GATC 3 cut(s) 176, 310, 639
Sau96I GGNCC 1 cut(s) 661
ScrFI CCNGG 1 cut(s) 625
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 1 cut(s) 769
Sse9I AATT 4 cut(s) 209, 406, 665, 810
SsiI CCGC 5 cut(s) 154, 168, 201, 330, 721
SspI AATATT 1 cut(s) 270
SspMI CTAG 2 cut(s) 458, 791
StyD4I CCNGG 1 cut(s) 623
TaaI ACNGT 2 cut(s) 359, 489
TaiI ACGT 1 cut(s) 395
TaqI TCGA 3 cut(s) 197, 422, 704
TasI AATT 4 cut(s) 209, 406, 665, 810
TauI GCSGC 2 cut(s) 204, 723
Tru1I TTAA 2 cut(s) 288, 336
Tru9I TTAA 2 cut(s) 288, 336
TscAI CASTG 2 cut(s) 492, 610
TseI GCWGC 2 cut(s) 226, 692
TspDTI ATGAA 2 cut(s) 486, 755
TspRI CASTG 2 cut(s) 492, 610
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 2 cut(s) 209, 810
XspI CTAG 2 cut(s) 458, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.