FvH4_2g36120

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012141
Physical Location & Seq
Forward (+)
26506146 .. 26508058
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36120.t4

Sequence Viewer

Length: 966 bp
ATGCTGTGGAGGAGAGGAGCAAGACACACAAAAGCGAATATCTTTCTCCCTTTCTCCGGTGAATCTGGAATCCCAAAATCCAGACTCAGAAAAATGGGTCAGTTTCAGGTCCACTATCTTCAAACCCTTCTCACTAAACCTCACTACACTTACTCCTCCCACCACCCAATCCCCATCATTTGCTTCTCTCACCGTCCCAACCTCACTCTCACCTTCCCTCACTCCCAAACTCACCCCAAACCCACTAAACCCCTCCACCTCTCTCTCCCCACCGCCGTCCGATCTCGCATACTCTGCAAATCCACCGGCTCAGAATTTGACATCCTATCACAGCCAGAAGAGAGAAGAAGGCCTCACAGACGCGCCAATGGAATCTTCTGGATTCTTCTCATTAATATTGGGGTATACTTGGCTGATCATATATTTCAGGTCCGTGCAGTTAAAATGCTCTACTTGCATCATAGTTACCCTGAATGGTACCAGTTTCTAACAGCGACATTCTGTCATGCTAATTGGAACCATCTTTCGAGCAATCTTTTCTTCTTGTATATTTTCGGAAAGCTAGTTGAAGAAGACGAAGGGAATCTCGCACTGTGGCTTTCTTATATTCTTACAGGTGTTGGAGCAAACCTTGTTTCGTGGCTTGTTTTACCGACGAATGCAGTTTCTATCGGAGCTTCTGGTGCTGTGTTTGGATTGTTTACTATCAGTGTCCTTGTAAAGATGTCCTGGGACTGGAGGAAGATCCTTGAAGTTCTTATATTGGGGCAATTTGTTTTAGACAAGGTTATGGAAGCAGCCCAAGCTTCTACTGGATTAGCAGGCCGCGGAGGCTCCTCTTTGATGAATGTCAATCATATTGCACATCTCTCGGGTGCTCTTGTTGGCGTATTTCTAGTATGGCTTCTTGGTAGAATTCCTTCAGAACCTCCAGAACAAGAAGGATTGGATCTGAAAAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

322

Amino Acids

36.32

Weight (kDa)

9.8

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 158 - 253 9.9e-22 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25640
fragaria_vesca FvH4_2g36120 FvH4_2g36120 FvH4_2g36120 FvH4_2g36120
malus_domestica MD15G1097700.v1.1
prunus_persica Prupe.1G452600_v2.0.a1
pyrus_communis pycom08g09880 pycom15g08960
rosa_chinensis RchiOBHm_Chr6g0305751
rosa_laevigata RLG00000010862
rosa_multiflora Rmu_sc0042960.1_g000002
rosa_roxburghii Rroxscaffold_7G00162380
rosa_rugosa Rorug06G0343900.1
rosa_samantha Rh6AG455300 Rh6BG446000 Rh6CG469200 Rh6DG455800
rosa_wichuraiana Rw6G039720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 477
AccB1I GGYRCC 1 cut(s) 477
AccI GTMKAC 1 cut(s) 405
AccII CGCG 2 cut(s) 363, 828
AciI CCGC 3 cut(s) 273, 826, 828
AclWI GGATC 2 cut(s) 739, 957
AcsI RAATTY 2 cut(s) 314, 915
AcuI CTGAAG 1 cut(s) 906
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 2 cut(s) 56, 735
AgsI TTSAA 3 cut(s) 122, 569, 752
AhdI GACNNNNNGTC 1 cut(s) 501
AjnI CCWGG 1 cut(s) 728
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 3 cut(s) 562, 677, 806
AluI AGCT 3 cut(s) 562, 677, 806
Alw21I GWGCWC 1 cut(s) 880
AlwI GGATC 2 cut(s) 739, 957
Ama87I CYCGRG 1 cut(s) 871
AoxI GGCC 2 cut(s) 350, 823
ApeKI GCWGC 1 cut(s) 797
ApoI RAATTY 2 cut(s) 314, 915
AseI ATTAAT 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 919
Asp718I GGTACC 1 cut(s) 477
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 2 cut(s) 109, 430
AsuHPI GGTGA 4 cut(s) 71, 182, 202, 224
AvaI CYCGRG 1 cut(s) 871
AvaII GGWCC 2 cut(s) 109, 430
BanI GGYRCC 1 cut(s) 477
BarI GAAGNNNNNNTAC 2 cut(s) 904, 936
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 880
BbvI GCAGC 1 cut(s) 809
BccI CCATC 2 cut(s) 182, 528
BceAI ACGGC 1 cut(s) 260
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 1 cut(s) 730
BclI TGATCA 1 cut(s) 415
BfaI CTAG 2 cut(s) 563, 896
BglI GCCNNNNNGGC 1 cut(s) 831
BisI GCNGC 2 cut(s) 798, 826
BlsI GCNGC 2 cut(s) 799, 827
Bme1390I CCNGG 1 cut(s) 730
Bme18I GGWCC 2 cut(s) 109, 430
BmeRI GACNNNNNGTC 1 cut(s) 501
BmeT110I CYCGRG 1 cut(s) 871
BmgT120I GGNCC 2 cut(s) 109, 430
BmiI GGNNCC 3 cut(s) 479, 518, 835
BmrFI CCNGG 1 cut(s) 730
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 579
BpmI CTGGAG 2 cut(s) 757, 915
BsaJI CCNNGG 2 cut(s) 729, 826
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 735
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 3 cut(s) 481, 740, 817
BseBI CCWGG 1 cut(s) 730
BseDI CCNNGG 2 cut(s) 729, 826
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 56, 735
BseMII CTCAG 2 cut(s) 100, 324
BseNI ACTGG 3 cut(s) 481, 740, 817
BseRI GAGGAG 4 cut(s) 25, 30, 145, 826
BseXI GCAGC 1 cut(s) 809
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 2 cut(s) 363, 828
BshFI GGCC 2 cut(s) 352, 825
BshNI GGYRCC 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 880
BsiHKCI CYCGRG 1 cut(s) 871
BsiSI CCGG 2 cut(s) 57, 306
BslFI GGGAC 2 cut(s) 180, 746
BslI CCNNNNNNNGG 2 cut(s) 56, 735
BsmFI GGGAC 2 cut(s) 180, 746
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 2 cut(s) 352, 825
BsoBI CYCGRG 1 cut(s) 871
Bsp1286I GDGCHC 1 cut(s) 880
Bsp143I GATC 4 cut(s) 281, 415, 744, 949
BspACI CCGC 3 cut(s) 273, 826, 828
BspANI GGCC 2 cut(s) 352, 825
BspCNI CTCAG 2 cut(s) 99, 323
BspFNI CGCG 2 cut(s) 363, 828
BspLI GGNNCC 3 cut(s) 479, 518, 835
BspPI GGATC 2 cut(s) 739, 957
BspT107I GGYRCC 1 cut(s) 477
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 3 cut(s) 481, 740, 817
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 2 cut(s) 729, 826
BssMI GATC 4 cut(s) 281, 415, 744, 949
BssNAI GTATAC 1 cut(s) 406
Bst1107I GTATAC 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 730
Bst4CI ACNGT 2 cut(s) 194, 594
Bst6I CTCTTC 1 cut(s) 333
BstAPI GCANNNNNTGC 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 823
BstDEI CTNAG 2 cut(s) 86, 310
BstDSI CCRYGG 1 cut(s) 826
BstF5I GGATG 1 cut(s) 321
BstFNI CGCG 2 cut(s) 363, 828
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 4 cut(s) 284, 418, 747, 952
BstMBI GATC 4 cut(s) 281, 415, 744, 949
BstMWI GCNNNNNNNGC 5 cut(s) 294, 454, 683, 803, 831
BstNI CCWGG 1 cut(s) 730
BstSCI CCNGG 1 cut(s) 728
BstUI CGCG 2 cut(s) 363, 828
BstV1I GCAGC 1 cut(s) 809
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 2 cut(s) 744, 949
BstYI RGATCY 2 cut(s) 744, 949
BstZ17I GTATAC 1 cut(s) 406
BsuRI GGCC 2 cut(s) 352, 825
BtgI CCRYGG 1 cut(s) 826
BtsCI GGATG 1 cut(s) 321
BtsIMutI CAGTG 2 cut(s) 590, 715
Cac8I GCNNGC 1 cut(s) 823
CfoI GCGC 1 cut(s) 365
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 2 cut(s) 109, 430
Cfr42I CCGCGG 1 cut(s) 829
CseI GACGC 1 cut(s) 369
Csp6I GTAC 1 cut(s) 478
CviAII CATG 1 cut(s) 506
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 2 cut(s) 86, 310
DpnI GATC 4 cut(s) 283, 417, 746, 951
DpnII GATC 4 cut(s) 281, 415, 744, 949
DriI GACNNNNNGTC 1 cut(s) 501
Eam1104I CTCTTC 1 cut(s) 333
Eam1105I GACNNNNNGTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 109, 430
Eco57I CTGAAG 1 cut(s) 906
Eco88I CYCGRG 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 915
EcoRII CCWGG 1 cut(s) 728
FaeI CATG 1 cut(s) 509
FaqI GGGAC 2 cut(s) 180, 746
FatI CATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 2 cut(s) 798, 826
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 2 cut(s) 798, 826
FspBI CTAG 2 cut(s) 563, 896
GlaI GCGC 1 cut(s) 364
GluI GCNGC 2 cut(s) 798, 826
GsuI CTGGAG 2 cut(s) 757, 915
HaeIII GGCC 2 cut(s) 352, 825
HapII CCGG 2 cut(s) 57, 306
HgaI GACGC 1 cut(s) 369
HhaI GCGC 1 cut(s) 365
Hin1II CATG 1 cut(s) 509
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HindIII AAGCTT 1 cut(s) 804
HinfI GANTC 6 cut(s) 62, 69, 84, 372, 382, 583
HpaII CCGG 2 cut(s) 57, 306
HphI GGTGA 4 cut(s) 71, 182, 202, 224
Hpy166II GTNNAC 3 cut(s) 112, 406, 702
Hpy188I TCNGA 7 cut(s) 89, 281, 313, 557, 674, 925, 954
Hpy188III TCNNGA 4 cut(s) 66, 81, 379, 932
Hpy8I GTNNAC 3 cut(s) 112, 406, 702
Hpy99I CGWCG 1 cut(s) 658
HpyAV CCTTC 6 cut(s) 137, 223, 342, 572, 930, 935
HpyCH4III ACNGT 2 cut(s) 194, 594
HpyCH4V TGCA 5 cut(s) 297, 437, 457, 662, 863
HpyF10VI GCNNNNNNNGC 5 cut(s) 294, 454, 683, 803, 831
HpyF3I CTNAG 2 cut(s) 86, 310
Hsp92II CATG 1 cut(s) 509
HspAI GCGC 1 cut(s) 363
KpnI GGTACC 1 cut(s) 481
Ksp22I TGATCA 1 cut(s) 415
KspI CCGCGG 1 cut(s) 829
Kzo9I GATC 4 cut(s) 281, 415, 744, 949
LmnI GCTCC 4 cut(s) 17, 623, 674, 839
Lsp1109I GCAGC 1 cut(s) 809
LweI GCATC 1 cut(s) 466
MaeI CTAG 2 cut(s) 563, 896
MaeIII GTNAC 1 cut(s) 464
MalI GATC 4 cut(s) 283, 417, 746, 951
MboI GATC 4 cut(s) 281, 415, 744, 949
MboII GAAGA 9 cut(s) 110, 350, 357, 367, 377, 532, 581, 584, 754
MflI RGATCY 2 cut(s) 744, 949
MhlI GDGCHC 1 cut(s) 880
MluCI AATT 4 cut(s) 314, 511, 770, 915
MlyI GAGTC 1 cut(s) 78
MmeI TCCRAC 1 cut(s) 601
MroXI GAANNNNTTC 1 cut(s) 919
MseI TTAA 2 cut(s) 393, 441
MspA1I CMGCKG 1 cut(s) 828
MspI CCGG 2 cut(s) 57, 306
MspR9I CCNGG 1 cut(s) 730
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 1 cut(s) 730
MvnI CGCG 2 cut(s) 363, 828
MwoI GCNNNNNNNGC 5 cut(s) 294, 454, 683, 803, 831
NdeII GATC 4 cut(s) 281, 415, 744, 949
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 3 cut(s) 479, 518, 835
PceI AGGCCT 1 cut(s) 352
PctI GAATGC 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 919
PfeI GAWTC 5 cut(s) 62, 69, 372, 382, 583
PkrI GCNGC 2 cut(s) 799, 827
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PshBI ATTAAT 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 728
PspGI CCWGG 1 cut(s) 728
PspN4I GGNNCC 3 cut(s) 479, 518, 835
PspPI GGNCC 2 cut(s) 109, 430
PsuI RGATCY 2 cut(s) 744, 949
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
SacII CCGCGG 1 cut(s) 829
SaqAI TTAA 2 cut(s) 393, 441
SatI GCNGC 2 cut(s) 798, 826
Sau3AI GATC 4 cut(s) 281, 415, 744, 949
Sau96I GGNCC 2 cut(s) 109, 430
SchI GAGTC 1 cut(s) 78
ScrFI CCNGG 1 cut(s) 730
SduI GDGCHC 1 cut(s) 880
SfaNI GCATC 1 cut(s) 466
Sfr303I CCGCGG 1 cut(s) 829
SgrBI CCGCGG 1 cut(s) 829
SinI GGWCC 2 cut(s) 109, 430
Sse9I AATT 4 cut(s) 314, 511, 770, 915
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 3 cut(s) 273, 826, 828
SspI AATATT 1 cut(s) 397
SspMI CTAG 2 cut(s) 563, 896
StuI AGGCCT 1 cut(s) 352
StyD4I CCNGG 1 cut(s) 728
TaaI ACNGT 2 cut(s) 194, 594
TaqI TCGA 1 cut(s) 527
TasI AATT 4 cut(s) 314, 511, 770, 915
TauI GCSGC 1 cut(s) 828
TfiI GAWTC 5 cut(s) 62, 69, 372, 382, 583
Tru1I TTAA 2 cut(s) 393, 441
Tru9I TTAA 2 cut(s) 393, 441
TscAI CASTG 2 cut(s) 597, 715
TseI GCWGC 1 cut(s) 797
TspDTI ATGAA 1 cut(s) 860
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 2 cut(s) 597, 715
VpaK11BI GGWCC 2 cut(s) 109, 430
VspI ATTAAT 1 cut(s) 393
XapI RAATTY 2 cut(s) 314, 915
XcmI CCANNNNNNNNNTGG 1 cut(s) 809
XmiI GTMKAC 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 919
XspI CTAG 2 cut(s) 563, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.