Rw6G039720

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012141
Physical Location & Seq
Reverse (-)
63655693 .. 63657361
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039720.1

Sequence Viewer

Length: 915 bp
ATGGGTCAGTTTCAGGTCCACCATGTTCAAACCCTCACTAAACCTCACTACTCTTACTCATCCCAGTCCCTCCATCCAGTCCCAATCACTTGCTTCCCTCATCGTATTCGTCCCAACCTCACTCTCACCTTCCCTCATTCCCTCTCTACTTCCCAAAACCACCCCAAACCCCTCCACCTCCCTCTCCGCACCGCCGTCCGATCTCGCATAGTCTGCAAATCGAGCGGCTCAGAATTTGACATCCTATCGCAGCCAGAAGAGAGAAGAAGACCTCACAGACGCGCCAATGGAATATTCTGGATTTTACTTATTAATATCGGAATATATTTGGCCGATCATATTTTTCAGGTTCGCGCAGTTAAAATGCTATACTTGTATCACAGTTACCCTGAATGGTACCAGTTTCTAACAGCAACGTTTGTCATGCTAATTGGTGAGACCCTTATAACTCAGAACCATCTTTCGAGCAACCTTTTCTTCTTGTATATTTTCGGAAAGCTAGTTGAAGAAGATGAAGGAAATCTCGCACTGTGGCTCTCTTATATTCTTACAGGTGTTGGAGCTAACTTTGTTTCGTGGCTTGCTCTACCGACAAATGCAGTTTCTATTGGAGCTTCTGGTGCTGTGTTTGGATTGTTTACTATCAGTGTTCTTGTAAAGATGTCCTGGGACTGGAGGAAGATACTTGAAGTTCTTATATTGGGCCAATTTGTTATAGACAAGGTTATGGAAGCAGCCCAAGCTTCGACAGGATTAGCAGGCCGCGGAGGCTCCTCTTTGATGAATGTCAATCACATTGCGCATCTCTCAGGTGCTCTTGTTGGTGTATTTCTAGTATGGCTTCTTGGTAGAATTCCTTCTGACCTCCAAACAAGAAGGATTGGATTTGAAAAAGAAATAGACAGAGATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

304

Amino Acids

34.36

Weight (kDa)

9.39

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 131 - 284 8.7e-20 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25640
fragaria_vesca FvH4_2g36120 FvH4_2g36120 FvH4_2g36120 FvH4_2g36120
malus_domestica MD15G1097700.v1.1
prunus_persica Prupe.1G452600_v2.0.a1
pyrus_communis pycom08g09880 pycom15g08960
rosa_chinensis RchiOBHm_Chr6g0305751
rosa_laevigata RLG00000010862
rosa_multiflora Rmu_sc0042960.1_g000002
rosa_roxburghii Rroxscaffold_7G00162380
rosa_rugosa Rorug06G0343900.1
rosa_samantha Rh6AG455300 Rh6BG446000 Rh6CG469200 Rh6DG455800
rosa_wichuraiana Rw6G039720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
Acc16I TGCGCA 1 cut(s) 801
Acc65I GGTACC 1 cut(s) 396
AccB1I GGYRCC 1 cut(s) 396
AccBSI CCGCTC 1 cut(s) 225
AccII CGCG 3 cut(s) 282, 354, 765
AciI CCGC 5 cut(s) 187, 192, 225, 763, 765
AclI AACGTT 1 cut(s) 416
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 2 cut(s) 233, 852
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 1 cut(s) 672
AgsI TTSAA 4 cut(s) 29, 506, 689, 890
AjnI CCWGG 1 cut(s) 665
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 4 cut(s) 499, 563, 614, 743
AluI AGCT 4 cut(s) 499, 563, 614, 743
Alw21I GWGCWC 1 cut(s) 817
Alw26I GTCTC 1 cut(s) 431
AoxI GGCC 3 cut(s) 330, 703, 760
ApeKI GCWGC 2 cut(s) 250, 734
ApoI RAATTY 2 cut(s) 233, 852
AseI ATTAAT 1 cut(s) 312
Asp700I GAANNNNTTC 1 cut(s) 856
Asp718I GGTACC 1 cut(s) 396
AspLEI GCGC 3 cut(s) 284, 356, 802
AspS9I GGNCC 2 cut(s) 16, 703
AsuHPI GGTGA 2 cut(s) 118, 446
AvaII GGWCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 396
BarI GAAGNNNNNNTAC 2 cut(s) 841, 873
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 817
BbvI GCAGC 2 cut(s) 262, 746
BccI CCATC 2 cut(s) 81, 465
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 667
BcoDI GTCTC 1 cut(s) 431
BfaI CTAG 2 cut(s) 500, 833
BglI GCCNNNNNGGC 1 cut(s) 768
BisI GCNGC 4 cut(s) 226, 251, 735, 763
BlsI GCNGC 4 cut(s) 227, 252, 736, 764
Bme1390I CCNGG 1 cut(s) 667
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 703
BmiI GGNNCC 2 cut(s) 398, 772
BmrFI CCNGG 1 cut(s) 667
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 1 cut(s) 811
BmuI ACTGGG 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 694
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 2 cut(s) 666, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 672
Bse1I ACTGG 4 cut(s) 64, 77, 400, 677
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 1 cut(s) 667
BseDI CCNNGG 2 cut(s) 666, 763
BseGI GGATG 3 cut(s) 59, 73, 240
BseLI CCNNNNNNNGG 1 cut(s) 672
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 3 cut(s) 243, 464, 822
BseNI ACTGG 4 cut(s) 64, 77, 400, 677
BseRI GAGGAG 1 cut(s) 763
BseXI GCAGC 2 cut(s) 262, 746
Bsh1236I CGCG 3 cut(s) 282, 354, 765
BshFI GGCC 3 cut(s) 332, 705, 762
BshNI GGYRCC 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 817
BslFI GGGAC 4 cut(s) 52, 65, 96, 683
BslI CCNNNNNNNGG 1 cut(s) 672
BsmAI GTCTC 1 cut(s) 431
BsmFI GGGAC 4 cut(s) 52, 65, 96, 683
BsnI GGCC 3 cut(s) 332, 705, 762
Bso31I GGTCTC 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 817
Bsp143I GATC 3 cut(s) 200, 334, 907
BspACI CCGC 5 cut(s) 187, 192, 225, 763, 765
BspANI GGCC 3 cut(s) 332, 705, 762
BspCNI CTCAG 3 cut(s) 242, 463, 821
BspFNI CGCG 3 cut(s) 282, 354, 765
BspLI GGNNCC 2 cut(s) 398, 772
BspT107I GGYRCC 1 cut(s) 396
BspTNI GGTCTC 1 cut(s) 431
BsrBI CCGCTC 1 cut(s) 225
BsrDI GCAATG 1 cut(s) 795
BsrI ACTGG 4 cut(s) 64, 77, 400, 677
BssECI CCNNGG 2 cut(s) 666, 763
BssMI GATC 3 cut(s) 200, 334, 907
Bst2UI CCWGG 1 cut(s) 667
Bst4CI ACNGT 2 cut(s) 383, 531
Bst6I CTCTTC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 582, 760
BstDEI CTNAG 3 cut(s) 229, 450, 808
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 3 cut(s) 59, 73, 240
BstFNI CGCG 3 cut(s) 282, 354, 765
BstHHI GCGC 3 cut(s) 284, 356, 802
BstKTI GATC 3 cut(s) 203, 337, 910
BstMAI GTCTC 1 cut(s) 431
BstMBI GATC 3 cut(s) 200, 334, 907
BstMWI GCNNNNNNNGC 5 cut(s) 213, 222, 620, 740, 768
BstNI CCWGG 1 cut(s) 667
BstSCI CCNGG 1 cut(s) 665
BstUI CGCG 3 cut(s) 282, 354, 765
BstV1I GCAGC 2 cut(s) 262, 746
BstV2I GAAGAC 1 cut(s) 274
BsuRI GGCC 3 cut(s) 332, 705, 762
BtgI CCRYGG 1 cut(s) 763
BtsCI GGATG 3 cut(s) 59, 73, 240
BtsIMutI CAGTG 2 cut(s) 527, 652
Cac8I GCNNGC 2 cut(s) 582, 760
CfoI GCGC 3 cut(s) 284, 356, 802
Cfr13I GGNCC 2 cut(s) 16, 703
Cfr42I CCGCGG 1 cut(s) 766
CseI GACGC 1 cut(s) 288
Csp6I GTAC 1 cut(s) 397
CviAII CATG 2 cut(s) 23, 424
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 3 cut(s) 229, 450, 808
DpnI GATC 3 cut(s) 202, 336, 909
DpnII GATC 3 cut(s) 200, 334, 907
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 852
EcoRII CCWGG 1 cut(s) 665
FaeI CATG 2 cut(s) 26, 427
FaqI GGGAC 4 cut(s) 52, 65, 96, 683
FatI CATG 2 cut(s) 22, 423
Fnu4HI GCNGC 4 cut(s) 226, 251, 735, 763
FokI GGATG 3 cut(s) 46, 60, 227
Fsp4HI GCNGC 4 cut(s) 226, 251, 735, 763
FspBI CTAG 2 cut(s) 500, 833
FspI TGCGCA 1 cut(s) 801
GlaI GCGC 3 cut(s) 283, 355, 801
GluI GCNGC 4 cut(s) 226, 251, 735, 763
GsuI CTGGAG 1 cut(s) 694
HaeIII GGCC 3 cut(s) 332, 705, 762
HgaI GACGC 1 cut(s) 288
HhaI GCGC 3 cut(s) 284, 356, 802
Hin1II CATG 2 cut(s) 26, 427
Hin6I GCGC 3 cut(s) 282, 354, 800
HinP1I GCGC 3 cut(s) 282, 354, 800
HindIII AAGCTT 1 cut(s) 741
HphI GGTGA 2 cut(s) 118, 446
Hpy166II GTNNAC 2 cut(s) 19, 639
Hpy188I TCNGA 6 cut(s) 200, 232, 320, 453, 494, 862
Hpy188III TCNNGA 1 cut(s) 298
Hpy8I GTNNAC 2 cut(s) 19, 639
HpyAV CCTTC 4 cut(s) 139, 509, 867, 870
HpyCH4III ACNGT 2 cut(s) 383, 531
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 2 cut(s) 216, 599
HpyF10VI GCNNNNNNNGC 5 cut(s) 213, 222, 620, 740, 768
HpyF3I CTNAG 3 cut(s) 229, 450, 808
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 2 cut(s) 26, 427
HspAI GCGC 3 cut(s) 282, 354, 800
KpnI GGTACC 1 cut(s) 400
KspI CCGCGG 1 cut(s) 766
Kzo9I GATC 3 cut(s) 200, 334, 907
LmnI GCTCC 3 cut(s) 560, 611, 776
Lsp1109I GCAGC 2 cut(s) 262, 746
LweI GCATC 1 cut(s) 811
MaeI CTAG 2 cut(s) 500, 833
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 1 cut(s) 383
MalI GATC 3 cut(s) 202, 336, 909
MbiI CCGCTC 1 cut(s) 225
MboI GATC 3 cut(s) 200, 334, 907
MboII GAAGA 7 cut(s) 269, 276, 279, 469, 518, 521, 691
MhlI GDGCHC 1 cut(s) 817
MluCI AATT 4 cut(s) 233, 429, 707, 852
MmeI TCCRAC 1 cut(s) 538
MroXI GAANNNNTTC 1 cut(s) 856
MseI TTAA 2 cut(s) 312, 360
MspA1I CMGCKG 1 cut(s) 765
MspR9I CCNGG 1 cut(s) 667
MvaI CCWGG 1 cut(s) 667
MvnI CGCG 3 cut(s) 282, 354, 765
MwoI GCNNNNNNNGC 5 cut(s) 213, 222, 620, 740, 768
NdeII GATC 3 cut(s) 200, 334, 907
NlaIII CATG 2 cut(s) 26, 427
NlaIV GGNNCC 2 cut(s) 398, 772
NsbI TGCGCA 1 cut(s) 801
PdmI GAANNNNTTC 1 cut(s) 856
PkrI GCNGC 4 cut(s) 227, 252, 736, 764
PshBI ATTAAT 1 cut(s) 312
PsiI TTATAA 1 cut(s) 446
Psp1406I AACGTT 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 665
PspGI CCWGG 1 cut(s) 665
PspN4I GGNNCC 2 cut(s) 398, 772
PspPI GGNCC 2 cut(s) 16, 703
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
SacII CCGCGG 1 cut(s) 766
SaqAI TTAA 2 cut(s) 312, 360
SatI GCNGC 4 cut(s) 226, 251, 735, 763
Sau3AI GATC 3 cut(s) 200, 334, 907
Sau96I GGNCC 2 cut(s) 16, 703
ScrFI CCNGG 1 cut(s) 667
SduI GDGCHC 1 cut(s) 817
SfaNI GCATC 1 cut(s) 811
Sfr303I CCGCGG 1 cut(s) 766
SgrBI CCGCGG 1 cut(s) 766
SinI GGWCC 1 cut(s) 16
Sse9I AATT 4 cut(s) 233, 429, 707, 852
SsiI CCGC 5 cut(s) 187, 192, 225, 763, 765
SspI AATATT 1 cut(s) 294
SspMI CTAG 2 cut(s) 500, 833
StyD4I CCNGG 1 cut(s) 665
TaaI ACNGT 2 cut(s) 383, 531
TaiI ACGT 1 cut(s) 419
TaqI TCGA 3 cut(s) 221, 464, 746
TasI AATT 4 cut(s) 233, 429, 707, 852
TauI GCSGC 2 cut(s) 228, 765
Tru1I TTAA 2 cut(s) 312, 360
Tru9I TTAA 2 cut(s) 312, 360
TscAI CASTG 2 cut(s) 534, 652
TseI GCWGC 2 cut(s) 250, 734
TspDTI ATGAA 2 cut(s) 528, 797
TspRI CASTG 2 cut(s) 534, 652
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 312
XapI RAATTY 2 cut(s) 233, 852
XmnI GAANNNNTTC 1 cut(s) 856
XspI CTAG 2 cut(s) 500, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.