Rh6BG446000

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012141
Physical Location & Seq
Forward (+)
66183352 .. 66185005
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG446000.1

Sequence Viewer

Length: 879 bp
ATGGGTCAGTTTCAGGTCCACCATGTTCAAACCCTCACTAAACCTCACTACTCTTACTCATCCCAGTCCCACCATCCAGTCCCAATCACTTGCTTCCCTCATCGTCCCAACCTCACTCTCACCTTCCCTCATTCCCTCTCTACTTCCCAAAACCACCCCAAACCCCTCCACCTCCCTCTCCTCACCGCCGTCCGATCTCGCATAGTCTGCAAATCCAGCGACTCAGAATTTGACATCCTAGCGCAGCCAGAAGAGAGAAGAAGACCTCACAGACGCGCCAATGGAATATTCTGGATTTTACTTATTAATATCGGAATATACTTGGCCGATCATATTTTTCAGGTTCGCGCAGTTAAAATGCTCTACTTGTATCACAGTTACCCTGAATGGTACCAGTTTCTAACAGCAACGTTCTGTCATGCTAATTGGAACCATCTTTCGAGCAACCTTTTCTTCTTGTATATTTTCGGAAAGCTAGTTGAAGAAGATGAAGGAAATCTCGCACTGTGGCTCTCTTATATTCTTACAGGTGTTGGAGCCAACTTTGTTTCGTGGCTTGCTCTACCGACAAATGCAGTTTCTATGGGAGCTTCTGGTGCTGTTTTTGGATTGTTTACTATCAGTGTTCTTGTCAAGATGTCCTGGGACTGGAGGAAGATACTTGAAGTTCTTATATTGGGCCAATTTGTTATAGACAAGGTTATGGAAGCAGCCCAAGCTTCTACAGGATTAGCAGGCCACGGAGGCTCCTCTTTGATGAATGTCAATCACATTGCGCATCTCTCAGGTGCTCTTGTTGGTGTATTTCTAGTATGGCTTCTTGGTAGAGTTCCTTCTGAACCTCCAGAACAAGAAGGATTGGATTTGAAAAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

292

Amino Acids

32.9

Weight (kDa)

8.72

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 129 - 276 1.4e-26 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25640
fragaria_vesca FvH4_2g36120 FvH4_2g36120 FvH4_2g36120 FvH4_2g36120
malus_domestica MD15G1097700.v1.1
prunus_persica Prupe.1G452600_v2.0.a1
pyrus_communis pycom08g09880 pycom15g08960
rosa_chinensis RchiOBHm_Chr6g0305751
rosa_laevigata RLG00000010862
rosa_multiflora Rmu_sc0042960.1_g000002
rosa_roxburghii Rroxscaffold_7G00162380
rosa_rugosa Rorug06G0343900.1
rosa_samantha Rh6AG455300 Rh6BG446000 Rh6CG469200 Rh6DG455800
rosa_wichuraiana Rw6G039720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 777
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 390
AccII CGCG 2 cut(s) 276, 348
AciI CCGC 1 cut(s) 186
AclI AACGTT 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 1 cut(s) 227
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 648
AgsI TTSAA 4 cut(s) 29, 482, 665, 868
AjnI CCWGG 1 cut(s) 641
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 3 cut(s) 475, 590, 719
AluI AGCT 3 cut(s) 475, 590, 719
Alw21I GWGCWC 1 cut(s) 793
AoxI GGCC 3 cut(s) 324, 679, 736
ApeKI GCWGC 2 cut(s) 244, 710
ApoI RAATTY 1 cut(s) 227
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AseI ATTAAT 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 390
AspLEI GCGC 4 cut(s) 244, 278, 350, 778
AspS9I GGNCC 2 cut(s) 16, 679
AsuHPI GGTGA 2 cut(s) 112, 175
AvaII GGWCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 390
BarI GAAGNNNNNNTAC 2 cut(s) 817, 849
BbsI GAAGAC 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 2 cut(s) 256, 722
BccI CCATC 2 cut(s) 81, 441
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 643
BfaI CTAG 3 cut(s) 239, 476, 809
BfmI CTRYAG 1 cut(s) 723
BglI GCCNNNNNGGC 1 cut(s) 744
BisI GCNGC 2 cut(s) 245, 711
BlsI GCNGC 2 cut(s) 246, 712
Bme1390I CCNGG 1 cut(s) 643
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 679
BmiI GGNNCC 4 cut(s) 392, 431, 538, 748
BmrFI CCNGG 1 cut(s) 643
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 1 cut(s) 787
BmuI ACTGGG 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 268
BpmI CTGGAG 2 cut(s) 670, 828
BsaJI CCNNGG 2 cut(s) 642, 739
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 1 cut(s) 648
Bse1I ACTGG 4 cut(s) 64, 77, 394, 653
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 1 cut(s) 643
BseDI CCNNGG 2 cut(s) 642, 739
BseGI GGATG 3 cut(s) 59, 73, 234
BseLI CCNNNNNNNGG 1 cut(s) 648
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 2 cut(s) 237, 798
BseNI ACTGG 4 cut(s) 64, 77, 394, 653
BseRI GAGGAG 2 cut(s) 170, 739
BseXI GCAGC 2 cut(s) 256, 722
Bsh1236I CGCG 2 cut(s) 276, 348
BshFI GGCC 3 cut(s) 326, 681, 738
BshNI GGYRCC 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 793
BslFI GGGAC 4 cut(s) 52, 65, 90, 659
BslI CCNNNNNNNGG 1 cut(s) 648
BsmFI GGGAC 4 cut(s) 52, 65, 90, 659
BsnI GGCC 3 cut(s) 326, 681, 738
Bsp1286I GDGCHC 1 cut(s) 793
Bsp143I GATC 2 cut(s) 194, 328
BspACI CCGC 1 cut(s) 186
BspANI GGCC 3 cut(s) 326, 681, 738
BspCNI CTCAG 2 cut(s) 236, 797
BspFNI CGCG 2 cut(s) 276, 348
BspLI GGNNCC 4 cut(s) 392, 431, 538, 748
BspT107I GGYRCC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 771
BsrI ACTGG 4 cut(s) 64, 77, 394, 653
BssECI CCNNGG 2 cut(s) 642, 739
BssMI GATC 2 cut(s) 194, 328
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 2 cut(s) 377, 507
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 558, 736
BstDEI CTNAG 2 cut(s) 223, 784
BstDSI CCRYGG 1 cut(s) 739
BstF5I GGATG 3 cut(s) 59, 73, 234
BstFNI CGCG 2 cut(s) 276, 348
BstHHI GCGC 4 cut(s) 244, 278, 350, 778
BstKTI GATC 2 cut(s) 197, 331
BstMBI GATC 2 cut(s) 194, 328
BstMWI GCNNNNNNNGC 5 cut(s) 207, 216, 596, 716, 744
BstNI CCWGG 1 cut(s) 643
BstSCI CCNGG 1 cut(s) 641
BstSFI CTRYAG 1 cut(s) 723
BstUI CGCG 2 cut(s) 276, 348
BstV1I GCAGC 2 cut(s) 256, 722
BstV2I GAAGAC 1 cut(s) 268
BsuRI GGCC 3 cut(s) 326, 681, 738
BtgI CCRYGG 1 cut(s) 739
BtsCI GGATG 3 cut(s) 59, 73, 234
BtsIMutI CAGTG 2 cut(s) 503, 628
Cac8I GCNNGC 2 cut(s) 558, 736
CfoI GCGC 4 cut(s) 244, 278, 350, 778
Cfr13I GGNCC 2 cut(s) 16, 679
CseI GACGC 1 cut(s) 282
Csp6I GTAC 1 cut(s) 391
CviAII CATG 2 cut(s) 23, 419
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 2 cut(s) 223, 784
DpnI GATC 2 cut(s) 196, 330
DpnII GATC 2 cut(s) 194, 328
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 641
FaeI CATG 2 cut(s) 26, 422
FaqI GGGAC 4 cut(s) 52, 65, 90, 659
FatI CATG 2 cut(s) 22, 418
Fnu4HI GCNGC 2 cut(s) 245, 711
FokI GGATG 3 cut(s) 46, 60, 221
Fsp4HI GCNGC 2 cut(s) 245, 711
FspBI CTAG 3 cut(s) 239, 476, 809
FspI TGCGCA 1 cut(s) 777
GlaI GCGC 4 cut(s) 243, 277, 349, 777
GluI GCNGC 2 cut(s) 245, 711
GsuI CTGGAG 2 cut(s) 670, 828
HaeIII GGCC 3 cut(s) 326, 681, 738
HgaI GACGC 1 cut(s) 282
HhaI GCGC 4 cut(s) 244, 278, 350, 778
Hin1II CATG 2 cut(s) 26, 422
Hin6I GCGC 4 cut(s) 242, 276, 348, 776
HinP1I GCGC 4 cut(s) 242, 276, 348, 776
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 1 cut(s) 221
HphI GGTGA 2 cut(s) 112, 175
Hpy166II GTNNAC 2 cut(s) 19, 615
Hpy188I TCNGA 5 cut(s) 194, 226, 314, 470, 838
Hpy188III TCNNGA 3 cut(s) 292, 634, 845
Hpy8I GTNNAC 2 cut(s) 19, 615
HpyAV CCTTC 4 cut(s) 133, 485, 843, 848
HpyCH4III ACNGT 2 cut(s) 377, 507
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 2 cut(s) 210, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 216, 596, 716, 744
HpyF3I CTNAG 2 cut(s) 223, 784
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 2 cut(s) 26, 422
HspAI GCGC 4 cut(s) 242, 276, 348, 776
KpnI GGTACC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 194, 328
LmnI GCTCC 3 cut(s) 536, 587, 752
Lsp1109I GCAGC 2 cut(s) 256, 722
LweI GCATC 1 cut(s) 787
MaeI CTAG 3 cut(s) 239, 476, 809
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 377
MalI GATC 2 cut(s) 196, 330
MboI GATC 2 cut(s) 194, 328
MboII GAAGA 7 cut(s) 263, 270, 273, 445, 494, 497, 667
MhlI GDGCHC 1 cut(s) 793
MluCI AATT 3 cut(s) 227, 424, 683
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 514
MseI TTAA 2 cut(s) 306, 354
MspR9I CCNGG 1 cut(s) 643
MvaI CCWGG 1 cut(s) 643
MvnI CGCG 2 cut(s) 276, 348
MwoI GCNNNNNNNGC 5 cut(s) 207, 216, 596, 716, 744
NdeII GATC 2 cut(s) 194, 328
NlaIII CATG 2 cut(s) 26, 422
NlaIV GGNNCC 4 cut(s) 392, 431, 538, 748
NsbI TGCGCA 1 cut(s) 777
PkrI GCNGC 2 cut(s) 246, 712
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
PshBI ATTAAT 1 cut(s) 306
Psp1406I AACGTT 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 641
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 4 cut(s) 392, 431, 538, 748
PspPI GGNCC 2 cut(s) 16, 679
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SaqAI TTAA 2 cut(s) 306, 354
SatI GCNGC 2 cut(s) 245, 711
Sau3AI GATC 2 cut(s) 194, 328
Sau96I GGNCC 2 cut(s) 16, 679
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 643
SduI GDGCHC 1 cut(s) 793
SfaNI GCATC 1 cut(s) 787
SfcI CTRYAG 1 cut(s) 723
SinI GGWCC 1 cut(s) 16
Sse9I AATT 3 cut(s) 227, 424, 683
SsiI CCGC 1 cut(s) 186
SspI AATATT 1 cut(s) 288
SspMI CTAG 3 cut(s) 239, 476, 809
StyD4I CCNGG 1 cut(s) 641
TaaI ACNGT 2 cut(s) 377, 507
TaiI ACGT 1 cut(s) 413
TaqI TCGA 1 cut(s) 440
TasI AATT 3 cut(s) 227, 424, 683
Tru1I TTAA 2 cut(s) 306, 354
Tru9I TTAA 2 cut(s) 306, 354
TscAI CASTG 2 cut(s) 510, 628
TseI GCWGC 2 cut(s) 244, 710
TspDTI ATGAA 2 cut(s) 504, 773
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 2 cut(s) 510, 628
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 306
XapI RAATTY 1 cut(s) 227
XspI CTAG 3 cut(s) 239, 476, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.