pycom08g09880

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012141
Physical Location & Seq
Forward (+)
8200498 .. 8201983
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09880.2

Sequence Viewer

Length: 651 bp
ATGAAACAAACTGAACCGATGGAATCTCCCTTTCCCGGTCTATCTGGAATCCCTCAACGCTCTAAAACCCAGCAACCTCCACAACGGAAGCAGAATATGTCCCAGTTTCTCCTTCAGCTCCACAGTCTTCAAACACTCACTAAACCCAGCAAACTCCAATCCATACCCAGCACCTGTTTCTCTCACCATCCCAACCTCACCACTTTTCCTCAAAAACCCACCATACCCAGCAAACTCCACCTCTCTCTCCGCCCCCTTCTTCCCTCCACCGCTCGATTCCCCGTAGTAGTATGCAAATCCAACGGCTCAGGCCTAGACTTCGATGTTCTGTCACAGCCAGAAGAGCGAGGAGGGAAGCCGCATCGACGCGCCAACGGAATATTCTGGATTATACTTGCTAACATTGGAATTTACTTGGCTGATCATGTGTTTCAGTTGAAGAAGAGGAAGGGAATTTTGCACTGTGGCTTTGTTATATTCTTATCGGGGTTGAAGCAAACCTTGTTTTGTGACTTGTACTACCAAGAAATGCAGTTTCCATTGGAGCCTCTGGTGCTGTTTTTGGATTATTTACGTTCAGTGTCCTTGCCAAGATGTCTTGGGACTGGAGGAAGATCCTCGAAGTGCTTATATTGGGTCAATTTGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

217

Amino Acids

24.46

Weight (kDa)

9.83

Isoelectric Point (pI)

60.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25640
fragaria_vesca FvH4_2g36120 FvH4_2g36120 FvH4_2g36120 FvH4_2g36120
malus_domestica MD15G1097700.v1.1
prunus_persica Prupe.1G452600_v2.0.a1
pyrus_communis pycom08g09880 pycom15g08960
rosa_chinensis RchiOBHm_Chr6g0305751
rosa_laevigata RLG00000010862
rosa_multiflora Rmu_sc0042960.1_g000002
rosa_roxburghii Rroxscaffold_7G00162380
rosa_rugosa Rorug06G0343900.1
rosa_samantha Rh6AG455300 Rh6BG446000 Rh6CG469200 Rh6DG455800
rosa_wichuraiana Rw6G039720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 272
AccII CGCG 1 cut(s) 369
AciI CCGC 3 cut(s) 250, 270, 359
AclWI GGATC 1 cut(s) 609
AcsI RAATTY 2 cut(s) 408, 453
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 35
AgsI TTSAA 3 cut(s) 131, 439, 493
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
AlwI GGATC 1 cut(s) 609
AlwNI CAGNNNCTG 1 cut(s) 174
AoxI GGCC 1 cut(s) 310
ApoI RAATTY 2 cut(s) 408, 453
AspLEI GCGC 1 cut(s) 371
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 2 cut(s) 176, 190
BbsI GAAGAC 1 cut(s) 119
BccI CCATC 2 cut(s) 13, 195
BceAI ACGGC 1 cut(s) 319
BclI TGATCA 1 cut(s) 421
BcnI CCSGG 1 cut(s) 36
BfaI CTAG 1 cut(s) 314
BisI GCNGC 1 cut(s) 359
BlsI GCNGC 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 36
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 1 cut(s) 36
BmrI ACTGGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 370
BmuI ACTGGG 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 119
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 307
BpuMI CCSGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse1I ACTGG 2 cut(s) 103, 610
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 103, 610
BseRI GAGGAG 1 cut(s) 363
BseYI CCCAGC 4 cut(s) 69, 146, 167, 227
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 312
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 2 cut(s) 85, 616
BslI CCNNNNNNNGG 1 cut(s) 35
BsmFI GGGAC 2 cut(s) 85, 616
BsnI GGCC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 421, 614
BspACI CCGC 3 cut(s) 250, 270, 359
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 320
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 546
BspPI GGATC 1 cut(s) 609
BspQI GCTCTTC 1 cut(s) 336
BsrBI CCGCTC 1 cut(s) 272
BsrI ACTGG 2 cut(s) 103, 610
BssMI GATC 2 cut(s) 421, 614
Bst4CI ACNGT 2 cut(s) 125, 464
Bst6I CTCTTC 2 cut(s) 336, 437
BstDEI CTNAG 1 cut(s) 307
BstF5I GGATG 1 cut(s) 187
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 2 cut(s) 424, 617
BstMBI GATC 2 cut(s) 421, 614
BstMWI GCNNNNNNNGC 2 cut(s) 343, 553
BstSCI CCNGG 1 cut(s) 34
BstUI CGCG 1 cut(s) 369
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
BsuRI GGCC 1 cut(s) 312
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 460, 585
CaiI CAGNNNCTG 1 cut(s) 174
CfoI GCGC 1 cut(s) 371
CseI GACGC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 517
CviAII CATG 1 cut(s) 425
CviJI RGCY 8 cut(s) 118, 306, 312, 337, 358, 419, 468, 547
CviKI_1 RGCY 8 cut(s) 118, 306, 312, 337, 358, 419, 468, 547
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 1 cut(s) 307
DpnI GATC 2 cut(s) 423, 616
DpnII GATC 2 cut(s) 421, 614
Eam1104I CTCTTC 2 cut(s) 336, 437
EarI CTCTTC 2 cut(s) 336, 437
EciI GGCGGA 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 98
FaeI CATG 1 cut(s) 428
FaiI YATR 9 cut(s) 98, 164, 224, 292, 392, 426, 476, 631, 649
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 2 cut(s) 85, 616
FatI CATG 1 cut(s) 424
FbaI TGATCA 1 cut(s) 421
Fnu4HI GCNGC 1 cut(s) 359
FokI GGATG 1 cut(s) 174
Fsp4HI GCNGC 1 cut(s) 359
FspBI CTAG 1 cut(s) 314
GlaI GCGC 1 cut(s) 370
GluI GCNGC 1 cut(s) 359
GsaI CCCAGC 4 cut(s) 73, 150, 171, 231
GsuI CTGGAG 1 cut(s) 627
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 375
HhaI GCGC 1 cut(s) 371
Hin1II CATG 1 cut(s) 428
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HinfI GANTC 3 cut(s) 23, 48, 276
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 176, 190
Hpy188III TCNNGA 2 cut(s) 45, 385
Hpy99I CGWCG 1 cut(s) 369
HpyAV CCTTC 3 cut(s) 122, 266, 442
HpyCH4III ACNGT 2 cut(s) 125, 464
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 3 cut(s) 294, 460, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 343, 553
HpyF3I CTNAG 1 cut(s) 307
HpySE526I ACGT 1 cut(s) 574
Hsp92II CATG 1 cut(s) 428
HspAI GCGC 1 cut(s) 369
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 2 cut(s) 421, 614
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 2 cut(s) 123, 544
LweI GCATC 1 cut(s) 370
MaeI CTAG 1 cut(s) 314
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 2 cut(s) 330, 509
MalI GATC 2 cut(s) 423, 616
MbiI CCGCTC 1 cut(s) 272
MboI GATC 2 cut(s) 421, 614
MboII GAAGA 6 cut(s) 119, 251, 353, 451, 454, 624
MflI RGATCY 1 cut(s) 614
MluCI AATT 3 cut(s) 408, 453, 640
MmeI TCCRAC 1 cut(s) 324
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 36
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 2 cut(s) 343, 553
NciI CCSGG 1 cut(s) 36
NdeII GATC 2 cut(s) 421, 614
NlaIII CATG 1 cut(s) 428
NlaIV GGNNCC 1 cut(s) 546
NmuCI GTSAC 2 cut(s) 330, 509
PceI AGGCCT 1 cut(s) 312
PciSI GCTCTTC 1 cut(s) 336
PfeI GAWTC 3 cut(s) 23, 48, 276
PkrI GCNGC 1 cut(s) 360
PspFI CCCAGC 4 cut(s) 69, 146, 167, 227
PspN4I GGNNCC 1 cut(s) 546
PstNI CAGNNNCTG 1 cut(s) 174
PsuI RGATCY 1 cut(s) 614
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 1 cut(s) 359
Sau3AI GATC 2 cut(s) 421, 614
ScrFI CCNGG 1 cut(s) 36
SetI ASST 7 cut(s) 79, 120, 176, 198, 243, 503, 577
SfaNI GCATC 1 cut(s) 370
Sse9I AATT 3 cut(s) 408, 453, 640
SseBI AGGCCT 1 cut(s) 312
SsiI CCGC 3 cut(s) 250, 270, 359
SspI AATATT 1 cut(s) 381
SspMI CTAG 1 cut(s) 314
StuI AGGCCT 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 34
TaaI ACNGT 2 cut(s) 125, 464
TaiI ACGT 1 cut(s) 577
TaqI TCGA 4 cut(s) 274, 321, 364, 620
TasI AATT 3 cut(s) 408, 453, 640
TatI WGTACW 1 cut(s) 516
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 3 cut(s) 23, 48, 276
TscAI CASTG 2 cut(s) 467, 585
TseFI GTSAC 2 cut(s) 330, 509
Tsp45I GTSAC 2 cut(s) 330, 509
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 100, 390
TspRI CASTG 2 cut(s) 467, 585
XapI RAATTY 2 cut(s) 408, 453
XspI CTAG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.