AT5G25780

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011162
Physical Location & Seq
Reverse (-)
8973247 .. 8976913
3667 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25780.1

Sequence Viewer

Length: 2145 bp
ATGGCGGCGGTTGTTGATATTGATGTCCTAACAGCTCAGTTAGGGATTAATTGGTCAGATGTTAATCTCGATTCAATCCAACTTCCTCCTGGAAACGACTTTGGGATCGAAAGTGATGATGAAAGTGTTTACCAAGATGACCAGTCAGAATTCGACACTGGATTCGGTAACATTATCGTTGTCGATCACCTCCCTGTTGTTCCCAAGGAGAAGTTTGAGAAACTAGAAGGTGTTGTTAAGAAAATTTACAACCAGTTAGGTGTGATTAAGGAAAATGGACTGTTGATGCCCGTTGATCCCGATACTAAGATGACATTGGGCTACTGTTTTATTGAGTTCAACACTCCTCAGGAAGCACAAAACGCTAAGGAAAAGTCTCATGGCTACAAGTTGGACAAGTCTCACATATTTGCTGTTAATATGTTCGATGATTTCGATAGGTTGATGAATGTGAAGGAGGAGTGGGAGCCTCCTCAAGAAAGGCCCTATGTCCCTGGGGAAAATTTGCAGAAGTGGCTTACTGATGACAAAGCCCGGGACCAGCTAGTCATTCGTTTTGGCCATGATACTGAAGTTTACTGGAATGATGCTAGGCAGAAGAAGCCTGAACCTGTTCATAAGCGTTCTTATTGGACAGAGAGTTATGTGCAGTGGTCTCCAATAGGTACATACCTTGTGACACTTCACAAGCAGGGGGCAGCTGTTTGGGGTGGTGCTGATACATTTACGCGTCTCATGCGTTATCAGCATTCAATGGTCAAACTAGTTGATTTCTCGCCTGGTGAGAAATATCTTGTAACTTACCATAGCCAAGAACCAAGCAATCCGAGAGATGCAAGTAAAGTAGAGATTAAGGTCTTTGATGTGAGAACCGGGAGGATGATGAGAGATTTCAAGGGTAGTGCTGATGAGTTTTCAATTGGAGGACCTGGTGGTGTTGCTGGTGCCTCTTGGCCTGTTTTCAGATGGGCTGGCGGAAAAGATGATAAATACTTTGCCAAGCTGAGCAAAAACACTATCTCTGTCTATGAGACTGAGACTTTCAGCCTAATAGACAAGAAATCCATGAAGGTTGATAATGTTGTGGATATTTGCTGGTCTCCCACTGATTCAATCCTTTCATTGTTTGTTCCTGAACAAGGTGGTGGAAACCAACCAGCCAAGGTTGCTCTAGTCCAAATCCCGAGTAAGGTAGAGCTGAGGCAGAAGAATCTCTTCAGTGTGAGCGACTGCAAGATGTACTGGCAGAGTAGTGGAGAGTATCTTGCTGTTAAGGTTGATCGATACACAAAAACTAAGAAGAGTACATACTCTGGATTTGAGCTTTTCCGTATCAAAGAAAGGGATATTCCAATCGAGGTTCTTGAGCTGGATAACAAGAACGATAAGATCATTGCTTTCGCGTGGGAGCCCAAGGGTCACAGGTTTGCTGTGATTCATGGTGACCAGCCAAGACCGGATGTTAGTTTCTACTCGATGAAGACTGCACAAAACACTGGAAGAGTTTCAAAGCTCGCAACTTTGAAGGCCAAACAAGCCAATGCCCTCTTCTGGTCGCCCACAGGCAAGTACATCATTCTTGCTGGGTTAAAAGGTTTCAATGGCCAGCTTGAATTTTTCAACGTGGATGAGCTCGAGACAATGGCCACAGCTGAACACTTCATGGCCACAGATATCGAATGGGACCCAACTGGAAGGTACGTTGCAACCTCAGTGACAACAGTCCACGAGATGGAGAATGGGTTCACCATATGGTCTTTCAATGGGAATATGGTTTACCGGATACTGAAGGATCATTTCTTCCAGTTGGCTTGGCGTCCAAGACCGGCATCATTCTTGACTGCGGAGAAGGAAGAAGAGATAGCAAAGAACCTGAGGAATTACAGCAAGAGGTATGAGGCAGAGGATCAGGACGTGTCGTTGCTATTGAGTGAACAAGACAGAGAGAAGAGGAGGGCATTGAATGAAGAGTGGCAGAAATGGGTGATGCAGTGGAAGTCTCTGCATGAAGAGGAGAAACTCGTGAGGCAAAATCTTCGGGATGGTGAAGTTAGTGATGTGGAAGAAGATGAGTATGAAGCCAAAGAAGTTGAGTTTGAGGATCTTATTGATGTTACTGAGGAAATTGTTCAAGAGTTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

714

Amino Acids

82.18

Weight (kDa)

5.11

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 80 - 134 7.2e-06 RNA recognition motif
eIF2A PF08662 403 - 603 2.2e-63 Eukaryotic translation initiation factor eIF2A
Beta-prop_THOC3 PF25174 461 - 571 1.2e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25780 AT5G27640 AT5G27640 AT5G27640
fragaria_vesca FvH4_6g00800
malus_domestica MD04G1241100.v1.1 MD12G1258100.v1.1
prunus_persica Prupe.6G358600_v2.0.a1
pyrus_communis pycom04g21280 pycom12g23670
rosa_chinensis RchiOBHm_Chr3g0448281
rosa_laevigata RLG00000025930
rosa_multiflora Rmu_sc0005582.1_g000005
rosa_roxburghii Rroxscaffold_6G00426430
rosa_rugosa Rorug02G0610000
rosa_samantha Rh3AG009600 Rh3BG009200 Rh3CG009200 Rh3DG009800
rosa_wichuraiana Rw3G000780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 944
AccB7I CCANNNNNTGG 1 cut(s) 1732
AccII CGCG 2 cut(s) 730, 1403
AciI CCGC 4 cut(s) 5, 8, 975, 1844
AclWI GGATC 5 cut(s) 113, 290, 1800, 1914, 2109
AcoI YGGCCR 4 cut(s) 559, 1603, 1644, 1665
AcsI RAATTY 4 cut(s) 149, 243, 502, 1613
AcuI CTGAAG 3 cut(s) 591, 1201, 1808
AcyI GRCGYC 1 cut(s) 1816
AfaI GTAC 5 cut(s) 667, 1241, 1306, 1571, 1700
AfiI CCNNNNNNNGG 4 cut(s) 1139, 1189, 1551, 1732
AflIII ACRYGT 2 cut(s) 728, 1914
AhlI ACTAGT 1 cut(s) 763
AjiI CACGTC 1 cut(s) 1915
AjnI CCWGG 4 cut(s) 88, 493, 778, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 1532, 1564
AloI GAACNNNNNNTCC 2 cut(s) 1727, 1759
Alw21I GWGCWC 1 cut(s) 1635
Alw26I GTCTC 9 cut(s) 381, 405, 660, 737, 1025, 1031, 1104, 1631, 2004
AlwI GGATC 5 cut(s) 113, 290, 1800, 1914, 2109
Ama87I CYCGRG 3 cut(s) 534, 1183, 1634
AoxI GGCC 7 cut(s) 482, 559, 953, 1527, 1603, 1644, 1665
ApeKI GCWGC 1 cut(s) 698
ApoI RAATTY 4 cut(s) 149, 243, 502, 1613
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AseI ATTAAT 1 cut(s) 48
Asp700I GAANNNNTTC 5 cut(s) 612, 1214, 1504, 1742, 2127
AspS9I GGNCC 4 cut(s) 483, 538, 926, 1684
AsuC2I CCSGG 3 cut(s) 535, 536, 874
AsuHPI GGTGA 6 cut(s) 179, 794, 1454, 1738, 1996, 2057
AvaI CYCGRG 3 cut(s) 534, 1183, 1634
AvaII GGWCC 3 cut(s) 538, 926, 1684
AxyI CCTNAGG 2 cut(s) 348, 1874
BaeI ACNNNNGTAYC 2 cut(s) 558, 591
BalI TGGCCA 4 cut(s) 561, 1605, 1646, 1667
BanI GGYRCC 1 cut(s) 944
BanII GRGCYC 2 cut(s) 1413, 1635
BarI GAAGNNNNNNTAC 2 cut(s) 1292, 1324
BauI CACGAG 2 cut(s) 1727, 2021
BbsI GAAGAC 1 cut(s) 1487
Bbv12I GWGCWC 1 cut(s) 1635
BbvCI CCTCAGC 1 cut(s) 1199
BbvI GCAGC 1 cut(s) 710
BccI CCATC 3 cut(s) 960, 1726, 2036
BcgI CGANNNNNNTGC 2 cut(s) 2018, 2052
BciT130I CCWGG 4 cut(s) 90, 495, 780, 930
BciVI GTATCC 1 cut(s) 1776
BcnI CCSGG 3 cut(s) 535, 536, 874
BcoDI GTCTC 9 cut(s) 381, 405, 660, 737, 1025, 1031, 1104, 1631, 2004
BcuI ACTAGT 1 cut(s) 763
BfaI CTAG 5 cut(s) 224, 545, 591, 764, 1172
BfuI GTATCC 1 cut(s) 1776
BisI GCNGC 2 cut(s) 6, 699
BlpI GCTNAGC 1 cut(s) 1004
BlsI GCNGC 2 cut(s) 7, 700
Bme1390I CCNGG 7 cut(s) 90, 495, 535, 536, 780, 874, 930
Bme18I GGWCC 3 cut(s) 538, 926, 1684
BmeT110I CYCGRG 3 cut(s) 534, 1183, 1634
BmgBI CACGTC 1 cut(s) 1915
BmgT120I GGNCC 4 cut(s) 483, 538, 926, 1684
BmiI GGNNCC 6 cut(s) 468, 539, 946, 1410, 1685, 1686
BmrFI CCNGG 7 cut(s) 90, 495, 535, 536, 780, 874, 930
BmsI GCATC 5 cut(s) 276, 577, 823, 1838, 1977
BoxI GACNNNNGTC 1 cut(s) 1721
BpiI GAAGAC 1 cut(s) 1487
Bpu10I CCTNAGC 2 cut(s) 366, 1199
Bpu1102I GCTNAGC 1 cut(s) 1004
BpuEI CTTGAG 2 cut(s) 459, 1385
BpuMI CCSGG 3 cut(s) 535, 536, 874
Bsa29I ATCGAT 1 cut(s) 1282
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 1816
BsaI GGTCTC 2 cut(s) 660, 1104
BsaJI CCNNGG 6 cut(s) 204, 493, 494, 534, 1161, 1413
BsaWI WCCGGW 2 cut(s) 1456, 1779
BsaXI ACNNNNNCTCC 2 cut(s) 1727, 1757
Bsc4I CCNNNNNNNGG 4 cut(s) 1139, 1189, 1551, 1732
Bse118I RCCGGY 1 cut(s) 1825
Bse1I ACTGG 8 cut(s) 142, 163, 253, 584, 1247, 1501, 1696, 1804
Bse21I CCTNAGG 2 cut(s) 348, 1874
Bse3DI GCAATG 1 cut(s) 1392
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 4 cut(s) 90, 495, 780, 930
BseCI ATCGAT 1 cut(s) 1282
BseDI CCNNGG 6 cut(s) 204, 493, 494, 534, 1161, 1413
BseGI GGATG 4 cut(s) 885, 1465, 1633, 2047
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 4 cut(s) 1139, 1189, 1551, 1732
BseMI GCAATG 1 cut(s) 1392
BseMII CTCAG 8 cut(s) 50, 362, 995, 1026, 1190, 1725, 1865, 2109
BseNI ACTGG 8 cut(s) 142, 163, 253, 584, 1247, 1501, 1696, 1804
BseRI GAGGAG 5 cut(s) 336, 462, 473, 1966, 2027
BseXI GCAGC 1 cut(s) 710
BseYI CCCAGC 1 cut(s) 1583
BsgI GTGCAG 2 cut(s) 668, 1470
Bsh1236I CGCG 2 cut(s) 730, 1403
BshFI GGCC 7 cut(s) 484, 561, 955, 1529, 1605, 1646, 1667
BshNI GGYRCC 1 cut(s) 944
BshVI ATCGAT 1 cut(s) 1282
BsiHKAI GWGCWC 1 cut(s) 1635
BsiHKCI CYCGRG 3 cut(s) 534, 1183, 1634
BsiSI CCGG 5 cut(s) 535, 873, 1457, 1780, 1826
BslFI GGGAC 3 cut(s) 476, 551, 1697
BslI CCNNNNNNNGG 4 cut(s) 1139, 1189, 1551, 1732
BsmAI GTCTC 9 cut(s) 381, 405, 660, 737, 1025, 1031, 1104, 1631, 2004
BsmBI CGTCTC 1 cut(s) 737
BsmFI GGGAC 3 cut(s) 476, 551, 1697
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 7 cut(s) 484, 561, 955, 1529, 1605, 1646, 1667
Bso31I GGTCTC 2 cut(s) 660, 1104
BsoBI CYCGRG 3 cut(s) 534, 1183, 1634
Bsp1286I GDGCHC 2 cut(s) 1413, 1635
Bsp143I GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
Bsp1720I GCTNAGC 1 cut(s) 1004
BspACI CCGC 4 cut(s) 5, 8, 975, 1844
BspANI GGCC 7 cut(s) 484, 561, 955, 1529, 1605, 1646, 1667
BspCNI CTCAG 8 cut(s) 49, 361, 996, 1027, 1191, 1724, 1866, 2110
BspDI ATCGAT 1 cut(s) 1282
BspFNI CGCG 2 cut(s) 730, 1403
BspLI GGNNCC 6 cut(s) 468, 539, 946, 1410, 1685, 1686
BspPI GGATC 5 cut(s) 113, 290, 1800, 1914, 2109
BspT107I GGYRCC 1 cut(s) 944
BspTNI GGTCTC 2 cut(s) 660, 1104
BsrDI GCAATG 1 cut(s) 1392
BsrFI RCCGGY 1 cut(s) 1825
BsrI ACTGG 8 cut(s) 142, 163, 253, 584, 1247, 1501, 1696, 1804
BssAI RCCGGY 1 cut(s) 1825
BssECI CCNNGG 6 cut(s) 204, 493, 494, 534, 1161, 1413
BssMI GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
BssNI GRCGYC 1 cut(s) 1816
BssSI CACGAG 2 cut(s) 1727, 2021
BssT1I CCWWGG 3 cut(s) 204, 1161, 1413
Bst2BI CACGAG 2 cut(s) 1727, 2021
Bst2UI CCWGG 4 cut(s) 90, 495, 780, 930
Bst4CI ACNGT 3 cut(s) 282, 326, 1723
Bst6I CTCTTC 8 cut(s) 1220, 1295, 1495, 1553, 1851, 1943, 1962, 2004
BstACI GRCGYC 1 cut(s) 1816
BstC8I GCNNGC 3 cut(s) 973, 1515, 1607
BstEII GGTNACC 1 cut(s) 1442
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstF5I GGATG 4 cut(s) 885, 1465, 1633, 2047
BstFNI CGCG 2 cut(s) 730, 1403
BstKTI GATC 8 cut(s) 108, 187, 298, 1282, 1392, 1795, 1909, 2104
BstMAI GTCTC 9 cut(s) 381, 405, 660, 737, 1025, 1031, 1104, 1631, 2004
BstMBI GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
BstMWI GCNNNNNNNGC 7 cut(s) 362, 514, 601, 736, 745, 1166, 1535
BstNI CCWGG 4 cut(s) 90, 495, 780, 930
BstPAI GACNNNNGTC 1 cut(s) 1721
BstPI GGTNACC 1 cut(s) 1442
BstSCI CCNGG 7 cut(s) 88, 493, 533, 534, 778, 872, 928
BstUI CGCG 2 cut(s) 730, 1403
BstV1I GCAGC 1 cut(s) 710
BstV2I GAAGAC 1 cut(s) 1487
BstX2I RGATCY 1 cut(s) 2101
BstYI RGATCY 1 cut(s) 2101
Bsu15I ATCGAT 1 cut(s) 1282
Bsu36I CCTNAGG 2 cut(s) 348, 1874
BsuI GTATCC 1 cut(s) 1776
BsuRI GGCC 7 cut(s) 484, 561, 955, 1529, 1605, 1646, 1667
BsuTUI ATCGAT 1 cut(s) 1282
BtrI CACGTC 1 cut(s) 1915
BtsCI GGATG 4 cut(s) 885, 1465, 1633, 2047
BtsI GCAGTG 2 cut(s) 656, 1997
BtsIMutI CAGTG 7 cut(s) 156, 656, 1104, 1225, 1494, 1719, 1997
Cac8I GCNNGC 3 cut(s) 973, 1515, 1607
Cfr10I RCCGGY 1 cut(s) 1825
Cfr13I GGNCC 4 cut(s) 483, 538, 926, 1684
Cfr9I CCCGGG 1 cut(s) 534
ClaI ATCGAT 1 cut(s) 1282
CseI GACGC 2 cut(s) 719, 1805
CsiI ACCWGGT 1 cut(s) 928
Csp6I GTAC 5 cut(s) 666, 1240, 1305, 1570, 1699
CviAII CATG 7 cut(s) 380, 563, 736, 1066, 1439, 1663, 2006
CviQI GTAC 5 cut(s) 666, 1240, 1305, 1570, 1699
DpnI GATC 8 cut(s) 107, 186, 297, 1281, 1391, 1794, 1908, 2103
DpnII GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 4 cut(s) 559, 1603, 1644, 1665
Eam1104I CTCTTC 8 cut(s) 1220, 1295, 1495, 1553, 1851, 1943, 1962, 2004
EarI CTCTTC 8 cut(s) 1220, 1295, 1495, 1553, 1851, 1943, 1962, 2004
EciI GGCGGA 1 cut(s) 990
Ecl136II GAGCTC 1 cut(s) 1633
Eco130I CCWWGG 3 cut(s) 204, 1161, 1413
Eco24I GRGCYC 2 cut(s) 1413, 1635
Eco31I GGTCTC 2 cut(s) 660, 1104
Eco32I GATATC 1 cut(s) 1675
Eco47I GGWCC 3 cut(s) 538, 926, 1684
Eco53kI GAGCTC 1 cut(s) 1633
Eco57I CTGAAG 3 cut(s) 591, 1201, 1808
Eco81I CCTNAGG 2 cut(s) 348, 1874
Eco88I CYCGRG 3 cut(s) 534, 1183, 1634
Eco91I GGTNACC 1 cut(s) 1442
EcoICRI GAGCTC 1 cut(s) 1633
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoO109I RGGNCCY 3 cut(s) 483, 926, 1684
EcoO65I GGTNACC 1 cut(s) 1442
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 4 cut(s) 88, 493, 778, 928
EcoRV GATATC 1 cut(s) 1675
EcoT14I CCWWGG 3 cut(s) 204, 1161, 1413
EcoT38I GRGCYC 2 cut(s) 1413, 1635
ErhI CCWWGG 3 cut(s) 204, 1161, 1413
Esp3I CGTCTC 1 cut(s) 737
FaeI CATG 7 cut(s) 383, 566, 739, 1069, 1442, 1666, 2009
FalI AAGNNNNNCTT 2 cut(s) 1199, 1231
FaqI GGGAC 3 cut(s) 476, 551, 1697
FatI CATG 7 cut(s) 379, 562, 735, 1065, 1438, 1662, 2005
FauNDI CATATG 1 cut(s) 1751
Fnu4HI GCNGC 2 cut(s) 6, 699
FokI GGATG 4 cut(s) 892, 1472, 1640, 2054
FriOI GRGCYC 2 cut(s) 1413, 1635
Fsp4HI GCNGC 2 cut(s) 6, 699
FspBI CTAG 5 cut(s) 224, 545, 591, 764, 1172
GluI GCNGC 2 cut(s) 6, 699
GsaI CCCAGC 1 cut(s) 1587
HaeIII GGCC 7 cut(s) 484, 561, 955, 1529, 1605, 1646, 1667
HapII CCGG 5 cut(s) 535, 873, 1457, 1780, 1826
HgaI GACGC 2 cut(s) 719, 1805
Hin1I GRCGYC 1 cut(s) 1816
Hin1II CATG 7 cut(s) 383, 566, 739, 1069, 1442, 1666, 2009
HinfI GANTC 5 cut(s) 71, 162, 1109, 1210, 1435
HpaII CCGG 5 cut(s) 535, 873, 1457, 1780, 1826
HphI GGTGA 6 cut(s) 179, 794, 1454, 1738, 1996, 2057
Hpy166II GTNNAC 6 cut(s) 130, 577, 1726, 1746, 1777, 1934
Hpy188I TCNGA 4 cut(s) 58, 148, 828, 965
Hpy8I GTNNAC 6 cut(s) 130, 577, 1726, 1746, 1777, 1934
HpyAV CCTTC 7 cut(s) 221, 448, 1063, 1519, 1689, 1783, 1843
HpyCH4III ACNGT 3 cut(s) 282, 326, 1723
HpyCH4IV ACGT 3 cut(s) 1623, 1701, 1914
HpyCH4V TGCA 8 cut(s) 508, 649, 836, 1233, 1487, 1706, 1990, 2005
HpyF10VI GCNNNNNNNGC 7 cut(s) 362, 514, 601, 736, 745, 1166, 1535
HpySE526I ACGT 3 cut(s) 1623, 1701, 1914
Hsp92I GRCGYC 1 cut(s) 1816
Hsp92II CATG 7 cut(s) 383, 566, 739, 1069, 1442, 1666, 2009
KflI GGGWCCC 1 cut(s) 1684
Kzo9I GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
LmnI GCTCC 2 cut(s) 466, 1408
Lsp1109I GCAGC 1 cut(s) 710
LweI GCATC 5 cut(s) 276, 577, 823, 1838, 1977
MabI ACCWGGT 1 cut(s) 928
MaeI CTAG 5 cut(s) 224, 545, 591, 764, 1172
MaeII ACGT 3 cut(s) 1623, 1701, 1914
MaeIII GTNAC 7 cut(s) 167, 676, 796, 1418, 1442, 1714, 2113
MalI GATC 8 cut(s) 107, 186, 297, 1281, 1391, 1794, 1908, 2103
MboI GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 1 cut(s) 2101
MhlI GDGCHC 2 cut(s) 1413, 1635
MlsI TGGCCA 4 cut(s) 561, 1605, 1646, 1667
MluCI AATT 8 cut(s) 49, 149, 243, 502, 918, 1613, 1879, 2124
MluI ACGCGT 1 cut(s) 728
MluNI TGGCCA 4 cut(s) 561, 1605, 1646, 1667
MmeI TCCRAC 2 cut(s) 103, 372
Mox20I TGGCCA 4 cut(s) 561, 1605, 1646, 1667
MroXI GAANNNNTTC 5 cut(s) 612, 1214, 1504, 1742, 2127
MscI TGGCCA 4 cut(s) 561, 1605, 1646, 1667
MseI TTAA 8 cut(s) 48, 63, 237, 267, 417, 852, 1272, 1589
Msp20I TGGCCA 4 cut(s) 561, 1605, 1646, 1667
MspA1I CMGCKG 2 cut(s) 701, 1652
MspI CCGG 5 cut(s) 535, 873, 1457, 1780, 1826
MspR9I CCNGG 7 cut(s) 90, 495, 535, 536, 780, 874, 930
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 4 cut(s) 90, 495, 780, 930
MvnI CGCG 2 cut(s) 730, 1403
MwoI GCNNNNNNNGC 7 cut(s) 362, 514, 601, 736, 745, 1166, 1535
NciI CCSGG 3 cut(s) 535, 536, 874
NdeI CATATG 1 cut(s) 1751
NdeII GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
NlaIII CATG 7 cut(s) 383, 566, 739, 1069, 1442, 1666, 2009
NlaIV GGNNCC 6 cut(s) 468, 539, 946, 1410, 1685, 1686
NmuCI GTSAC 4 cut(s) 676, 1418, 1442, 1714
PaeR7I CTCGAG 1 cut(s) 1634
PasI CCCWGGG 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PctI GAATGC 1 cut(s) 748
PdmI GAANNNNTTC 5 cut(s) 612, 1214, 1504, 1742, 2127
PfeI GAWTC 5 cut(s) 71, 162, 1109, 1210, 1435
PflMI CCANNNNNTGG 1 cut(s) 1732
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 2 cut(s) 7, 700
PpuMI RGGWCCY 2 cut(s) 926, 1684
PshAI GACNNNNGTC 1 cut(s) 1721
PshBI ATTAAT 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 1635
Psp5II RGGWCCY 2 cut(s) 926, 1684
Psp6I CCWGG 4 cut(s) 88, 493, 778, 928
PspEI GGTNACC 1 cut(s) 1442
PspFI CCCAGC 1 cut(s) 1583
PspGI CCWGG 4 cut(s) 88, 493, 778, 928
PspN4I GGNNCC 6 cut(s) 468, 539, 946, 1410, 1685, 1686
PspPI GGNCC 4 cut(s) 483, 538, 926, 1684
PspPPI RGGWCCY 2 cut(s) 926, 1684
PsuI RGATCY 1 cut(s) 2101
PvuII CAGCTG 2 cut(s) 701, 1652
RsaI GTAC 5 cut(s) 667, 1241, 1306, 1571, 1700
RsaNI GTAC 5 cut(s) 666, 1240, 1305, 1570, 1699
SacI GAGCTC 1 cut(s) 1635
SaqAI TTAA 8 cut(s) 48, 63, 237, 267, 417, 852, 1272, 1589
SatI GCNGC 2 cut(s) 6, 699
Sau3AI GATC 8 cut(s) 105, 184, 295, 1279, 1389, 1792, 1906, 2101
Sau96I GGNCC 4 cut(s) 483, 538, 926, 1684
ScrFI CCNGG 7 cut(s) 90, 495, 535, 536, 780, 874, 930
SduI GDGCHC 2 cut(s) 1413, 1635
SexAI ACCWGGT 1 cut(s) 928
SfaNI GCATC 5 cut(s) 276, 577, 823, 1838, 1977
Sfr274I CTCGAG 1 cut(s) 1634
SinI GGWCC 3 cut(s) 538, 926, 1684
SlaI CTCGAG 1 cut(s) 1634
SmaI CCCGGG 1 cut(s) 536
SmlI CTYRAG 3 cut(s) 474, 1364, 1634
SmoI CTYRAG 3 cut(s) 474, 1364, 1634
SpeI ACTAGT 1 cut(s) 763
Sse9I AATT 8 cut(s) 49, 149, 243, 502, 918, 1613, 1879, 2124
SsiI CCGC 4 cut(s) 5, 8, 975, 1844
SspMI CTAG 5 cut(s) 224, 545, 591, 764, 1172
SstI GAGCTC 1 cut(s) 1635
StyD4I CCNGG 7 cut(s) 88, 493, 533, 534, 778, 872, 928
StyI CCWWGG 3 cut(s) 204, 1161, 1413
TaaI ACNGT 3 cut(s) 282, 326, 1723
TaiI ACGT 3 cut(s) 1626, 1704, 1917
TasI AATT 8 cut(s) 49, 149, 243, 502, 918, 1613, 1879, 2124
TatI WGTACW 3 cut(s) 1239, 1304, 1569
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 5 cut(s) 71, 162, 1109, 1210, 1435
Tru1I TTAA 8 cut(s) 48, 63, 237, 267, 417, 852, 1272, 1589
Tru9I TTAA 8 cut(s) 48, 63, 237, 267, 417, 852, 1272, 1589
TscAI CASTG 7 cut(s) 163, 656, 1111, 1225, 1501, 1719, 1997
TseFI GTSAC 4 cut(s) 676, 1418, 1442, 1714
TseI GCWGC 1 cut(s) 698
Tsp45I GTSAC 4 cut(s) 676, 1418, 1442, 1714
TspGWI ACGGA 1 cut(s) 1319
TspMI CCCGGG 1 cut(s) 534
TspRI CASTG 7 cut(s) 163, 656, 1111, 1225, 1501, 1719, 1997
Van91I CCANNNNNTGG 1 cut(s) 1732
VpaK11BI GGWCC 3 cut(s) 538, 926, 1684
VspI ATTAAT 1 cut(s) 48
XagI CCTNNNNNAGG 1 cut(s) 1137
XapI RAATTY 4 cut(s) 149, 243, 502, 1613
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XhoI CTCGAG 1 cut(s) 1634
XmaI CCCGGG 1 cut(s) 534
XmnI GAANNNNTTC 5 cut(s) 612, 1214, 1504, 1742, 2127
XspI CTAG 5 cut(s) 224, 545, 591, 764, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.