Rroxscaffold_6G00426430

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011162
Physical Location & Seq
Reverse (-)
47168117 .. 47172267
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00426430.1

Sequence Viewer

Length: 2100 bp
ATGGCGGACGTGATGGTGATGAACGACATAGAGGCGACGGCGGCTCGTCTCGGAATTGACCTCTCGCAGTTAGACCTAGACGCAATCCGTCTCCCTCACGGCGAAACCTGCGGGATTCCAAGTGACGATGAAGATATCGGTCACGATGTGAATTCGGAGATGGAGTCCGGGTTCGGGAACATCATCGTTGTCGATAACCTGCCGGTGGTGCCGATTGAGAAGTTCGAGAAGCTTGAAAACGTGATTCGGAAAATTTACAGTCAGCTTGGTGTGATCAAGGAAGATGGATTCTGGATGCCTCTCGAACCCGAAACGCAGAAGACTATGGGCTATTGCTTCATTGAGTTCAATACTCCTCAGGAAGCTGAGCTTGCCAGGGAGAAGACTCATGGATACAAGCTGGACAGATCACACATTTTCGCAGTCAGCATGTTTGATGACTTTGATCGTTTCATGAAAGAAAATCTTCAAAATTGGCTCACTGATTTAAAAGGCCGGGATCAATTTGTCATCCGTGCTGGTAATGATACTGAAGTTTATTGGAATGATGCACGACAATTGAAGCCTGATTTTGTTTACAAGCGTCAATACTGGACTGAAAGCTTTGTGCAATGGTCACCCCTGGGGACTTATTTGGCCACTGTACACAGACAGGGTGCTGCAGTTTGGGGAGGTGCCACCAGCGAGTTCAAACGATTGATGCGTTATGCTCATCCACAGGTTAGACTGATTGATTTTTCCCCTGGTGAGAAATATTTGGTGACCTACAGCAGCCATGAAGCAAGCAATCCCCGCGACACAAATAGGGTTGTCATAGACATTTTTGATGTGAGAACTGGAAAAGTTATGAGAGATTTTAAGGGAACTCCTGATGATTTTGCAATTGGAGGAACTGGAGGTGTTTCGGGCGTGTCTTGGCCTGTGTTCAAATGGGGTGGCGGAAAAGATGACAAGTACTTTGCCAGAATGGGAAAGAGTGCTATATCTGTTTATGAGACAGAAACGTTTTCACTTGTGGACAAAAAATCTATAAAAGTTGAGAATGTTATGGACTTCAGTTGGTCACCAACTGATTCAATTCTTGCTCTTTTTGTTCCTGAATTGGGCGGCGGGAACCAACCTGCTAGGGTGAGTCTTATTCAAATCCCCAGTAAAGAGGAGTTAAGGCAGAAGAATCTTTTCAGTGTTAGTGATTGCAAGATGTACTGGCAAAGCAATGGGGAGTATCTTGCTGTTAAAGTTGATCGTTATACGAAAACGAAAAAAAGTACATATACTGGATTTGAACTTTTCCGAATCAAGGAGCGAGATATCCCTATTGAAGTTTTAGAGCTTGAGAATAAGAATGACAAGATCATTGCATTTGCTTGGGAGCCTAAGGGACATAGATTTGCAATTATTCATGGTGACACTCCAAGGCCTGATATAAGCTTCTACTCAATGCGTACTGCTCACAATACAGGCCGTGTGTCAAAGCTTATTACTCTTAAAGGCAAGCAGGCGAATGCTCTTTCTTGGTCACCTTCAGGGCGCTTTATAATTCTTGCAGGATTGAAGGGTTTGAACGGGCAGTTGGAATTTTACAATGTGGATGAGCAAGAAACGATGGCGACAACTGAGCATTTTATGGCGACAGATATTGAATGGGATCCTACTGGAAGATATGTTGCTACTGCAGTTACTTCAGTTCATGAGATGGAGAATGGTTATATTATATGGACCTTCAATGGCAAGCTACTTTATCGGGTATTAAAGGATCATTTCTTCCAGTTTTTGTGGCGTCCAAGGCCAGCATCTTTCCTGACTCCTGAGCAAGAGGAGGAGATTGCAAAGAACTTGAAAAAGTATAGCAAGAGATATGAGCTCGAGGATAATGATGTGGAAAAGCAATTGAGCGAGCAGGAGCGTGCAAAGAGGAGAGAGTTGAAGGAAGAATGGGAGAAGTGGGTCGCTGAGTGGAAGCGGTTGTACGAAGAAGAAAAAATGGATAGGCAGAGGCTCAGGGATGGAGAAGCCAGTGATGAAGAGGAGGAGTACGAAGCTAGAGAGGTTGAAGTAGAGGAAGTGGTGAGTACTCAAGAGGAGGTTCTTTCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

699

Amino Acids

80.84

Weight (kDa)

5.15

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 79 - 136 5e-06 RNA recognition motif
eIF2A PF08662 391 - 591 2.2e-62 Eukaryotic translation initiation factor eIF2A
Beta-prop_THOC3 PF25174 449 - 575 1e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25780 AT5G27640 AT5G27640 AT5G27640
fragaria_vesca FvH4_6g00800
malus_domestica MD04G1241100.v1.1 MD12G1258100.v1.1
prunus_persica Prupe.6G358600_v2.0.a1
pyrus_communis pycom04g21280 pycom12g23670
rosa_chinensis RchiOBHm_Chr3g0448281
rosa_laevigata RLG00000025930
rosa_multiflora Rmu_sc0005582.1_g000005
rosa_roxburghii Rroxscaffold_6G00426430
rosa_rugosa Rorug02G0610000
rosa_samantha Rh3AG009600 Rh3BG009200 Rh3CG009200 Rh3DG009800
rosa_wichuraiana Rw3G000780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1538
Acc36I ACCTGC 3 cut(s) 116, 207, 1129
AccB1I GGYRCC 2 cut(s) 208, 674
AccII CGCG 1 cut(s) 795
AciI CCGC 8 cut(s) 5, 41, 111, 793, 939, 1107, 1110, 1963
AclI AACGTT 1 cut(s) 1004
AclWI GGATC 4 cut(s) 507, 1643, 1656, 1764
AcoI YGGCCR 1 cut(s) 636
AcsI RAATTY 3 cut(s) 151, 252, 1577
AcuI CTGAAG 4 cut(s) 552, 1039, 1509, 1668
AcyI GRCGYC 1 cut(s) 1780
AdeI CACNNNGTG 1 cut(s) 148
AfaI GTAC 8 cut(s) 645, 956, 1205, 1270, 1447, 1970, 2036, 2074
AfiI CCNNNNNNNGG 4 cut(s) 174, 205, 1103, 1300
AjiI CACGTC 1 cut(s) 10
AjnI CCWGG 3 cut(s) 374, 621, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 1556, 1588
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
Alw21I GWGCWC 1 cut(s) 1866
Alw26I GTCTC 3 cut(s) 53, 95, 989
AlwI GGATC 4 cut(s) 507, 1643, 1656, 1764
Ama87I CYCGRG 1 cut(s) 1865
AoxI GGCC 6 cut(s) 493, 636, 917, 1418, 1462, 1787
ApeKI GCWGC 2 cut(s) 659, 771
ApoI RAATTY 3 cut(s) 151, 252, 1577
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
Asp700I GAANNNNTTC 2 cut(s) 465, 1178
AspLEI GCGC 1 cut(s) 1533
AspS9I GGNCC 1 cut(s) 1719
AsuC2I CCSGG 2 cut(s) 169, 497
AsuHPI GGTGA 9 cut(s) 28, 609, 758, 772, 1056, 1141, 1418, 1512, 2080
AvaI CYCGRG 1 cut(s) 1865
AvaII GGWCC 1 cut(s) 1719
AxyI CCTNAGG 2 cut(s) 357, 1377
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BalI TGGCCA 1 cut(s) 638
BamHI GGATCC 1 cut(s) 1648
BanI GGYRCC 2 cut(s) 208, 674
BanII GRGCYC 1 cut(s) 1866
BarI GAAGNNNNNNTAC 2 cut(s) 1952, 1984
BbsI GAAGAC 2 cut(s) 326, 389
Bbv12I GWGCWC 1 cut(s) 1866
BbvI GCAGC 2 cut(s) 646, 783
BccI CCATC 6 cut(s) 7, 154, 278, 1600, 1690, 2000
BceAI ACGGC 3 cut(s) 54, 115, 1449
BciT130I CCWGG 3 cut(s) 376, 623, 744
BciVI GTATCC 1 cut(s) 386
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 2 cut(s) 169, 497
BcoDI GTCTC 3 cut(s) 53, 95, 989
BfaI CTAG 3 cut(s) 77, 1125, 2043
BfmI CTRYAG 3 cut(s) 660, 766, 1674
BfoI RGCGCY 1 cut(s) 1534
BfuAI ACCTGC 3 cut(s) 116, 207, 1129
BfuI GTATCC 1 cut(s) 386
BisI GCNGC 4 cut(s) 42, 660, 772, 1108
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 4 cut(s) 43, 661, 773, 1109
BmcAI AGTACT 2 cut(s) 956, 2074
Bme1390I CCNGG 5 cut(s) 169, 376, 497, 623, 744
Bme18I GGWCC 1 cut(s) 1719
BmeT110I CYCGRG 1 cut(s) 1865
BmgBI CACGTC 1 cut(s) 10
BmgT120I GGNCC 1 cut(s) 1719
BmiI GGNNCC 5 cut(s) 210, 676, 1115, 1374, 1650
BmrFI CCNGG 5 cut(s) 169, 376, 497, 623, 744
BmrI ACTGGG 1 cut(s) 1143
BmsI GCATC 4 cut(s) 285, 538, 690, 1802
BmuI ACTGGG 1 cut(s) 1143
BpiI GAAGAC 2 cut(s) 326, 389
BpmI CTGGAG 1 cut(s) 915
Bpu10I CCTNAGC 2 cut(s) 1809, 2000
Bpu1102I GCTNAGC 1 cut(s) 366
BpuEI CTTGAG 2 cut(s) 1355, 2061
BpuMI CCSGG 2 cut(s) 169, 497
BsaHI GRCGYC 1 cut(s) 1780
BsaJI CCNNGG 6 cut(s) 375, 621, 622, 742, 1415, 1784
BsaXI ACNNNNNCTCC 4 cut(s) 149, 155, 179, 185
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 205, 1103, 1300
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 9 cut(s) 596, 841, 898, 1149, 1211, 1282, 1660, 1768, 2016
Bse21I CCTNAGG 2 cut(s) 357, 1377
Bse3DI GCAATG 3 cut(s) 617, 1222, 1356
BseBI CCWGG 3 cut(s) 376, 623, 744
BseDI CCNNGG 6 cut(s) 375, 621, 622, 742, 1415, 1784
BseGI GGATG 5 cut(s) 300, 510, 712, 1597, 2011
BseLI CCNNNNNNNGG 4 cut(s) 174, 205, 1103, 1300
BseMI GCAATG 3 cut(s) 617, 1222, 1356
BseMII CTCAG 6 cut(s) 357, 371, 1608, 1800, 1944, 2014
BseNI ACTGG 9 cut(s) 596, 841, 898, 1149, 1211, 1282, 1660, 1768, 2016
BseRI GAGGAG 8 cut(s) 345, 1172, 1832, 1835, 1930, 2042, 2045, 2096
BseXI GCAGC 2 cut(s) 646, 783
Bsh1236I CGCG 1 cut(s) 795
BshFI GGCC 6 cut(s) 495, 638, 919, 1420, 1464, 1789
BshNI GGYRCC 2 cut(s) 208, 674
BsiHKAI GWGCWC 1 cut(s) 1866
BsiHKCI CYCGRG 1 cut(s) 1865
BsiSI CCGG 3 cut(s) 168, 203, 496
BslFI GGGAC 2 cut(s) 640, 1395
BslI CCNNNNNNNGG 4 cut(s) 174, 205, 1103, 1300
BsmAI GTCTC 3 cut(s) 53, 95, 989
BsmBI CGTCTC 2 cut(s) 53, 95
BsmFI GGGAC 2 cut(s) 640, 1395
BsmI GAATGC 1 cut(s) 1510
BsnI GGCC 6 cut(s) 495, 638, 919, 1420, 1464, 1789
BsoBI CYCGRG 1 cut(s) 1865
Bsp1286I GDGCHC 1 cut(s) 1866
Bsp1407I TGTACA 1 cut(s) 643
Bsp143I GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 8 cut(s) 5, 41, 111, 793, 939, 1107, 1110, 1963
BspANI GGCC 6 cut(s) 495, 638, 919, 1420, 1464, 1789
BspCNI CTCAG 6 cut(s) 358, 370, 1609, 1801, 1945, 2013
BspFNI CGCG 1 cut(s) 795
BspHI TCATGA 2 cut(s) 453, 1690
BspLI GGNNCC 5 cut(s) 210, 676, 1115, 1374, 1650
BspMAI CTGCAG 2 cut(s) 664, 1678
BspMI ACCTGC 3 cut(s) 116, 207, 1129
BspPI GGATC 4 cut(s) 507, 1643, 1656, 1764
BspT107I GGYRCC 2 cut(s) 208, 674
BsrDI GCAATG 3 cut(s) 617, 1222, 1356
BsrFI RCCGGY 1 cut(s) 202
BsrGI TGTACA 1 cut(s) 643
BsrI ACTGG 9 cut(s) 596, 841, 898, 1149, 1211, 1282, 1660, 1768, 2016
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 6 cut(s) 375, 621, 622, 742, 1415, 1784
BssMI GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
BssNI GRCGYC 1 cut(s) 1780
BssT1I CCWWGG 2 cut(s) 1415, 1784
Bst2UI CCWGG 3 cut(s) 376, 623, 744
Bst4CI ACNGT 2 cut(s) 260, 643
Bst6I CTCTTC 1 cut(s) 2019
BstACI GRCGYC 1 cut(s) 1780
BstAUI TGTACA 1 cut(s) 643
BstC8I GCNNGC 8 cut(s) 372, 784, 1496, 1500, 1733, 1791, 1898, 1908
BstDEI CTNAG 7 cut(s) 357, 366, 1377, 1617, 1809, 1953, 2000
BstEII GGTNACC 4 cut(s) 615, 760, 1062, 1518
BstF5I GGATG 5 cut(s) 300, 510, 712, 1597, 2011
BstFNI CGCG 1 cut(s) 795
BstH2I RGCGCY 1 cut(s) 1534
BstHHI GCGC 1 cut(s) 1533
BstKTI GATC 8 cut(s) 276, 410, 448, 502, 1246, 1356, 1651, 1759
BstMAI GTCTC 3 cut(s) 53, 95, 989
BstMBI GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
BstMWI GCNNNNNNNGC 6 cut(s) 41, 108, 208, 371, 792, 1786
BstNI CCWGG 3 cut(s) 376, 623, 744
BstNSI RCATGY 1 cut(s) 433
BstPI GGTNACC 4 cut(s) 615, 760, 1062, 1518
BstSCI CCNGG 5 cut(s) 167, 374, 495, 621, 742
BstSFI CTRYAG 3 cut(s) 660, 766, 1674
BstUI CGCG 1 cut(s) 795
BstV1I GCAGC 2 cut(s) 646, 783
BstV2I GAAGAC 2 cut(s) 326, 389
BstX2I RGATCY 1 cut(s) 1648
BstYI RGATCY 1 cut(s) 1648
Bsu36I CCTNAGG 2 cut(s) 357, 1377
BsuI GTATCC 1 cut(s) 386
BsuRI GGCC 6 cut(s) 495, 638, 919, 1420, 1464, 1789
BtrI CACGTC 1 cut(s) 10
BtsCI GGATG 5 cut(s) 300, 510, 712, 1597, 2011
BtsIMutI CAGTG 4 cut(s) 480, 639, 1189, 2023
BveI ACCTGC 3 cut(s) 116, 207, 1129
Cac8I GCNNGC 8 cut(s) 372, 784, 1496, 1500, 1733, 1791, 1898, 1908
CciI TCATGA 2 cut(s) 453, 1690
CfoI GCGC 1 cut(s) 1533
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 1719
CseI GACGC 3 cut(s) 89, 572, 1769
Csp6I GTAC 8 cut(s) 644, 955, 1204, 1269, 1446, 1969, 2035, 2073
CspCI CAANNNNNGTGG 2 cut(s) 916, 951
CviAII CATG 6 cut(s) 389, 430, 454, 776, 1403, 1691
CviQI GTAC 8 cut(s) 644, 955, 1204, 1269, 1446, 1969, 2035, 2073
DdeI CTNAG 7 cut(s) 357, 366, 1377, 1617, 1809, 1953, 2000
DpnI GATC 8 cut(s) 275, 409, 447, 501, 1245, 1355, 1650, 1758
DpnII GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
DraI TTTAAA 1 cut(s) 489
DraIII CACNNNGTG 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 636
Eam1104I CTCTTC 1 cut(s) 2019
EarI CTCTTC 1 cut(s) 2019
EciI GGCGGA 2 cut(s) 20, 954
Ecl136II GAGCTC 1 cut(s) 1864
Eco130I CCWWGG 2 cut(s) 1415, 1784
Eco147I AGGCCT 1 cut(s) 1420
Eco24I GRGCYC 1 cut(s) 1866
Eco32I GATATC 2 cut(s) 136, 1312
Eco47I GGWCC 1 cut(s) 1719
Eco53kI GAGCTC 1 cut(s) 1864
Eco57I CTGAAG 4 cut(s) 552, 1039, 1509, 1668
Eco81I CCTNAGG 2 cut(s) 357, 1377
Eco88I CYCGRG 1 cut(s) 1865
Eco91I GGTNACC 4 cut(s) 615, 760, 1062, 1518
EcoICRI GAGCTC 1 cut(s) 1864
EcoO65I GGTNACC 4 cut(s) 615, 760, 1062, 1518
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 3 cut(s) 374, 621, 742
EcoRV GATATC 2 cut(s) 136, 1312
EcoT14I CCWWGG 2 cut(s) 1415, 1784
EcoT38I GRGCYC 1 cut(s) 1866
ErhI CCWWGG 2 cut(s) 1415, 1784
Esp3I CGTCTC 2 cut(s) 53, 95
FaeI CATG 6 cut(s) 392, 433, 457, 779, 1406, 1694
FalI AAGNNNNNCTT 4 cut(s) 354, 386, 450, 482
FaqI GGGAC 2 cut(s) 640, 1395
FatI CATG 6 cut(s) 388, 429, 453, 775, 1402, 1690
FauI CCCGC 3 cut(s) 104, 800, 1103
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 4 cut(s) 42, 660, 772, 1108
FokI GGATG 5 cut(s) 307, 497, 699, 1604, 2018
FriOI GRGCYC 1 cut(s) 1866
Fsp4HI GCNGC 4 cut(s) 42, 660, 772, 1108
FspBI CTAG 3 cut(s) 77, 1125, 2043
GlaI GCGC 1 cut(s) 1532
GluI GCNGC 4 cut(s) 42, 660, 772, 1108
GsuI CTGGAG 1 cut(s) 915
HaeII RGCGCY 1 cut(s) 1534
HaeIII GGCC 6 cut(s) 495, 638, 919, 1420, 1464, 1789
HapII CCGG 3 cut(s) 168, 203, 496
HgaI GACGC 3 cut(s) 89, 572, 1769
HhaI GCGC 1 cut(s) 1533
Hin1I GRCGYC 1 cut(s) 1780
Hin1II CATG 6 cut(s) 392, 433, 457, 779, 1406, 1694
Hin6I GCGC 1 cut(s) 1531
HinP1I GCGC 1 cut(s) 1531
HindIII AAGCTT 4 cut(s) 230, 601, 1429, 1475
HpaII CCGG 3 cut(s) 168, 203, 496
HphI GGTGA 9 cut(s) 28, 609, 758, 772, 1056, 1141, 1418, 1512, 2080
Hpy166II GTNNAC 3 cut(s) 577, 646, 1018
Hpy188I TCNGA 5 cut(s) 53, 157, 249, 1295, 2095
Hpy8I GTNNAC 3 cut(s) 577, 646, 1018
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 4 cut(s) 1533, 1549, 1732, 1921
HpyCH4III ACNGT 2 cut(s) 260, 643
HpyCH4IV ACGT 3 cut(s) 9, 240, 1004
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 108, 208, 371, 792, 1786
HpyF3I CTNAG 7 cut(s) 357, 366, 1377, 1617, 1809, 1953, 2000
HpySE526I ACGT 3 cut(s) 9, 240, 1004
Hsp92I GRCGYC 1 cut(s) 1780
Hsp92II CATG 6 cut(s) 392, 433, 457, 779, 1406, 1694
HspAI GCGC 1 cut(s) 1531
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
LmnI GCTCC 3 cut(s) 1303, 1372, 1903
Lsp1109I GCAGC 2 cut(s) 646, 783
LweI GCATC 4 cut(s) 285, 538, 690, 1802
MaeI CTAG 3 cut(s) 77, 1125, 2043
MaeII ACGT 3 cut(s) 9, 240, 1004
MaeIII GTNAC 8 cut(s) 122, 140, 615, 760, 1062, 1406, 1518, 1678
MalI GATC 8 cut(s) 275, 409, 447, 501, 1245, 1355, 1650, 1758
MboI GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
MfeI CAATTG 3 cut(s) 557, 882, 1889
MflI RGATCY 1 cut(s) 1648
MhlI GDGCHC 1 cut(s) 1866
MlsI TGGCCA 1 cut(s) 638
MluNI TGGCCA 1 cut(s) 638
MlyI GAGTC 4 cut(s) 173, 379, 1141, 1798
MmeI TCCRAC 1 cut(s) 1554
Mox20I TGGCCA 1 cut(s) 638
MroXI GAANNNNTTC 2 cut(s) 465, 1178
MscI TGGCCA 1 cut(s) 638
MseI TTAA 6 cut(s) 488, 858, 1163, 1236, 1488, 1751
Msp20I TGGCCA 1 cut(s) 638
MspI CCGG 3 cut(s) 168, 203, 496
MspR9I CCNGG 5 cut(s) 169, 376, 497, 623, 744
MunI CAATTG 3 cut(s) 557, 882, 1889
Mva1269I GAATGC 1 cut(s) 1510
MvaI CCWGG 3 cut(s) 376, 623, 744
MvnI CGCG 1 cut(s) 795
MwoI GCNNNNNNNGC 6 cut(s) 41, 108, 208, 371, 792, 1786
NciI CCSGG 2 cut(s) 169, 497
NdeII GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
NlaIII CATG 6 cut(s) 392, 433, 457, 779, 1406, 1694
NlaIV GGNNCC 5 cut(s) 210, 676, 1115, 1374, 1650
NmuCI GTSAC 7 cut(s) 122, 140, 615, 760, 1062, 1406, 1518
NspI RCATGY 1 cut(s) 433
PaeR7I CTCGAG 1 cut(s) 1865
PagI TCATGA 2 cut(s) 453, 1690
PasI CCCWGGG 1 cut(s) 622
PceI AGGCCT 1 cut(s) 1420
PcsI WCGNNNNNNNCGW 1 cut(s) 700
PctI GAATGC 1 cut(s) 1510
PdmI GAANNNNTTC 2 cut(s) 465, 1178
PfeI GAWTC 6 cut(s) 115, 244, 288, 1073, 1174, 1296
PkrI GCNGC 4 cut(s) 43, 661, 773, 1109
PleI GAGTC 4 cut(s) 172, 379, 1140, 1798
PpsI GAGTC 4 cut(s) 172, 379, 1140, 1798
PsiI TTATAA 1 cut(s) 1538
Psp124BI GAGCTC 1 cut(s) 1866
Psp1406I AACGTT 1 cut(s) 1004
Psp6I CCWGG 3 cut(s) 374, 621, 742
PspEI GGTNACC 4 cut(s) 615, 760, 1062, 1518
PspGI CCWGG 3 cut(s) 374, 621, 742
PspN4I GGNNCC 5 cut(s) 210, 676, 1115, 1374, 1650
PspPI GGNCC 1 cut(s) 1719
PspXI VCTCGAGB 1 cut(s) 1865
PstI CTGCAG 2 cut(s) 664, 1678
PsuI RGATCY 1 cut(s) 1648
RsaI GTAC 8 cut(s) 645, 956, 1205, 1270, 1447, 1970, 2036, 2074
RsaNI GTAC 8 cut(s) 644, 955, 1204, 1269, 1446, 1969, 2035, 2073
SacI GAGCTC 1 cut(s) 1866
SaqAI TTAA 6 cut(s) 488, 858, 1163, 1236, 1488, 1751
SatI GCNGC 4 cut(s) 42, 660, 772, 1108
Sau3AI GATC 8 cut(s) 273, 407, 445, 499, 1243, 1353, 1648, 1756
Sau96I GGNCC 1 cut(s) 1719
ScaI AGTACT 2 cut(s) 956, 2074
SchI GAGTC 4 cut(s) 173, 379, 1141, 1798
ScrFI CCNGG 5 cut(s) 169, 376, 497, 623, 744
SduI GDGCHC 1 cut(s) 1866
SfaNI GCATC 4 cut(s) 285, 538, 690, 1802
SfcI CTRYAG 3 cut(s) 660, 766, 1674
Sfr274I CTCGAG 1 cut(s) 1865
SinI GGWCC 1 cut(s) 1719
SlaI CTCGAG 1 cut(s) 1865
SmlI CTYRAG 3 cut(s) 1334, 1865, 2076
SmoI CTYRAG 3 cut(s) 1334, 1865, 2076
SseBI AGGCCT 1 cut(s) 1420
SsiI CCGC 8 cut(s) 5, 41, 111, 793, 939, 1107, 1110, 1963
SspI AATATT 1 cut(s) 755
SspMI CTAG 3 cut(s) 77, 1125, 2043
SstI GAGCTC 1 cut(s) 1866
StuI AGGCCT 1 cut(s) 1420
StyD4I CCNGG 5 cut(s) 167, 374, 495, 621, 742
StyI CCWWGG 2 cut(s) 1415, 1784
TaaI ACNGT 2 cut(s) 260, 643
TaiI ACGT 3 cut(s) 12, 243, 1007
TaqI TCGA 4 cut(s) 192, 225, 303, 1866
TaqII GACCGA 1 cut(s) 128
TatI WGTACW 5 cut(s) 643, 954, 1203, 1268, 2072
TauI GCSGC 2 cut(s) 44, 1110
TfiI GAWTC 6 cut(s) 115, 244, 288, 1073, 1174, 1296
Tru1I TTAA 6 cut(s) 488, 858, 1163, 1236, 1488, 1751
Tru9I TTAA 6 cut(s) 488, 858, 1163, 1236, 1488, 1751
TscAI CASTG 4 cut(s) 487, 646, 1189, 2023
TseFI GTSAC 7 cut(s) 122, 140, 615, 760, 1062, 1406, 1518
TseI GCWGC 2 cut(s) 659, 771
Tsp45I GTSAC 7 cut(s) 122, 140, 615, 760, 1062, 1406, 1518
TspDTI ATGAA 9 cut(s) 35, 144, 328, 442, 470, 792, 1391, 1679, 2037
TspGWI ACGGA 2 cut(s) 77, 503
TspRI CASTG 4 cut(s) 487, 646, 1189, 2023
VpaK11BI GGWCC 1 cut(s) 1719
XapI RAATTY 3 cut(s) 151, 252, 1577
XceI RCATGY 1 cut(s) 433
XhoI CTCGAG 1 cut(s) 1865
XmnI GAANNNNTTC 2 cut(s) 465, 1178
XspI CTAG 3 cut(s) 77, 1125, 2043
ZrmI AGTACT 2 cut(s) 956, 2074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.