Rorug02G0610000
ERF Family

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001369
Physical Location & Seq
Reverse (-)
72647818 .. 72692828
45011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0610000.1

Sequence Viewer

Length: 1044 bp
ATGGCAGGGAGGTCTTGTTACAGAGATGTGTTGCCCTTTGCAGCCATGGTTACAGTGGAGAGCACCAACGTTGGCTTAAACATCTTGTTCAAGTCAGCAACCACAAAAGGGTTGAGTTACTATGTGTTCATTGTCTATTCTTATGCTTTTGCCACTGTTCTTCTCCTCCCTCTTCCTTTCATTTTTCCCAGCAGAACAACGCTTCCTCCATTGAACTTCTCTCTTCTCTTTAAAGCTTTCCTCCTTGGACTGATCGGGTTTGTGGCAAATATATGTGCATATAAAGGTATAGATTACAGTTCACCAACTCTTGCTTCAGCTATGAGCAACCTCACTCCGGCTTTTACATTCATACTTGCCGTCATCTTCAGAATGGAAAGGCTGAGTGTGAGAAGCATCAGCACCCAAGCCAAAGTCATGGGTACTATAGTGTCAATATCAGGTGCTCTAGTGGTGGTTCTCTATAGAGGCCCAACAGTTCTATCCAATACATCAGAAAAGGATTGGGTAAAAAGTGGCATATTACTTACTGTTGGGTACCTTCTGTTCTCTACCTGGTCAATCCTTCAGACACATATCATGAAGACATACCCGGCTGAGCTAGTTTTGGCACTGTTATACAACTTGTTTGGAACAATTATATCAGCACCAGTGTGTGTACTAATAGCTGAAACAAAGTTAAGTGCCTGGAGACTAAGGCCTGGGGTAGCATTGGCTGCCATTATATACTCGGGGCTCTTTAGTTCAGCCTTGAGCACCCTTGTTAAGACATGGAGCCTAAACTTGAAAGGTCCTGTGTATGTATCAATCTTCAGCCCAATGTCAATTGTCATTGCTGCTGCATCCAGTGTCATATTCCTCGGTGATAAGCTCTTTCTCGGCAGTGTTGTTGGAGCTGTAATGATATGCATAGGGTTTTATGGGGTAATATGGGGACAGGCAAAGGAAGAAGAAATGAAAGATCAAGACTATGGCTTTGATAGCTTGAGAGGGTTACATGGTGGTAAGGCACCATTATTGGAAACCTACAAAGTTGAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

347

Amino Acids

37.69

Weight (kDa)

9.3

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 25 - 153 1.3e-10 EamA-like transporter family
EamA PF00892 174 - 310 2.1e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 537
AccB1I GGYRCC 2 cut(s) 537, 1009
AclI AACGTT 1 cut(s) 69
AcuI CTGAAG 4 cut(s) 300, 352, 551, 796
AfaI GTAC 3 cut(s) 424, 539, 660
AfiI CCNNNNNNNGG 2 cut(s) 108, 337
AgsI TTSAA 4 cut(s) 91, 214, 787, 1037
AjnI CCWGG 3 cut(s) 554, 686, 700
AjuI GAANNNNNNNTTGG 2 cut(s) 298, 330
AleI CACNNNNGTG 1 cut(s) 652
AloI GAACNNNNNNTCC 2 cut(s) 187, 219
AluBI AGCT 7 cut(s) 236, 320, 601, 668, 871, 896, 984
AluI AGCT 7 cut(s) 236, 320, 601, 668, 871, 896, 984
Alw21I GWGCWC 3 cut(s) 65, 448, 758
Alw26I GTCTC 1 cut(s) 685
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 2 cut(s) 469, 698
ApeKI GCWGC 4 cut(s) 41, 716, 836, 839
ArsI GACNNNNNNTTYG 2 cut(s) 959, 991
Asp718I GGTACC 1 cut(s) 537
AspS9I GGNCC 2 cut(s) 470, 791
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 2 cut(s) 294, 875
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 1 cut(s) 791
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BanI GGYRCC 2 cut(s) 537, 1009
BanII GRGCYC 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 590
Bbv12I GWGCWC 3 cut(s) 65, 448, 758
BbvI GCAGC 4 cut(s) 53, 703, 823, 826
BceAI ACGGC 1 cut(s) 344
BciT130I CCWGG 3 cut(s) 556, 688, 702
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 1 cut(s) 685
BfaI CTAG 2 cut(s) 449, 602
BfmI CTRYAG 2 cut(s) 426, 463
BisI GCNGC 4 cut(s) 42, 717, 837, 840
BlpI GCTNAGC 1 cut(s) 597
BlsI GCNGC 4 cut(s) 43, 718, 838, 841
Bme1390I CCNGG 4 cut(s) 556, 593, 688, 702
Bme18I GGWCC 1 cut(s) 791
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 2 cut(s) 470, 791
BmiI GGNNCC 3 cut(s) 539, 776, 1011
BmrFI CCNGG 4 cut(s) 556, 593, 688, 702
BmsI GCATC 2 cut(s) 405, 851
BpiI GAAGAC 1 cut(s) 590
BpmI CTGGAG 1 cut(s) 709
Bpu1102I GCTNAGC 1 cut(s) 597
BpuEI CTTGAG 2 cut(s) 772, 1006
BpuMI CCSGG 1 cut(s) 593
BsaJI CCNNGG 4 cut(s) 45, 244, 701, 859
BsaXI ACNNNNNCTCC 3 cut(s) 31, 190, 220
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 337
Bse1I ACTGG 2 cut(s) 650, 846
Bse3DI GCAATG 1 cut(s) 831
BseBI CCWGG 3 cut(s) 556, 688, 702
BseDI CCNNGG 4 cut(s) 45, 244, 701, 859
BseGI GGATG 1 cut(s) 842
BseLI CCNNNNNNNGG 2 cut(s) 108, 337
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 2 cut(s) 374, 588
BseNI ACTGG 2 cut(s) 650, 846
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 4 cut(s) 53, 703, 823, 826
BseYI CCCAGC 1 cut(s) 188
BshFI GGCC 2 cut(s) 471, 700
BshNI GGYRCC 2 cut(s) 537, 1009
BsiHKAI GWGCWC 3 cut(s) 65, 448, 758
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 2 cut(s) 338, 593
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 2 cut(s) 108, 337
BsmAI GTCTC 1 cut(s) 685
BsmFI GGGAC 1 cut(s) 948
BsnI GGCC 2 cut(s) 471, 700
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 4 cut(s) 65, 448, 738, 758
Bsp143I GATC 2 cut(s) 252, 961
Bsp1720I GCTNAGC 1 cut(s) 597
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 2 cut(s) 471, 700
BspCNI CTCAG 2 cut(s) 375, 589
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 3 cut(s) 539, 776, 1011
BspT107I GGYRCC 2 cut(s) 537, 1009
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 2 cut(s) 650, 846
BssECI CCNNGG 4 cut(s) 45, 244, 701, 859
BssMI GATC 2 cut(s) 252, 961
BssT1I CCWWGG 2 cut(s) 45, 244
Bst2UI CCWGG 3 cut(s) 556, 688, 702
Bst4CI ACNGT 6 cut(s) 55, 157, 299, 478, 532, 615
Bst6I CTCTTC 2 cut(s) 177, 228
BstAPI GCANNNNNTGC 1 cut(s) 716
BstDEI CTNAG 3 cut(s) 383, 597, 695
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 842
BstKTI GATC 2 cut(s) 255, 964
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 2 cut(s) 252, 961
BstMWI GCNNNNNNNGC 2 cut(s) 716, 981
BstNI CCWGG 3 cut(s) 556, 688, 702
BstSCI CCNGG 4 cut(s) 554, 591, 686, 700
BstSFI CTRYAG 2 cut(s) 426, 463
BstV1I GCAGC 4 cut(s) 53, 703, 823, 826
BstV2I GAAGAC 1 cut(s) 590
BstXI CCANNNNNNTGG 1 cut(s) 418
BsuRI GGCC 2 cut(s) 471, 700
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 842
BtsI GCAGTG 1 cut(s) 889
BtsIMutI CAGTG 6 cut(s) 60, 153, 611, 657, 853, 889
CciI TCATGA 1 cut(s) 579
Cfr13I GGNCC 2 cut(s) 470, 791
CsiI ACCWGGT 1 cut(s) 554
Csp6I GTAC 3 cut(s) 423, 538, 659
CviAII CATG 5 cut(s) 46, 418, 580, 771, 998
CviQI GTAC 3 cut(s) 423, 538, 659
DdeI CTNAG 3 cut(s) 383, 597, 695
DpnI GATC 2 cut(s) 254, 963
DpnII GATC 2 cut(s) 252, 961
DraI TTTAAA 1 cut(s) 232
Eam1104I CTCTTC 2 cut(s) 177, 228
EarI CTCTTC 2 cut(s) 177, 228
Eco130I CCWWGG 2 cut(s) 45, 244
Eco147I AGGCCT 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 738
Eco47I GGWCC 1 cut(s) 791
Eco57I CTGAAG 4 cut(s) 300, 352, 551, 796
Eco88I CYCGRG 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 791
EcoRII CCWGG 3 cut(s) 554, 686, 700
EcoT14I CCWWGG 2 cut(s) 45, 244
EcoT22I ATGCAT 1 cut(s) 911
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 2 cut(s) 45, 244
FaeI CATG 5 cut(s) 49, 421, 583, 774, 1001
FaqI GGGAC 1 cut(s) 948
FatI CATG 5 cut(s) 45, 417, 579, 770, 997
Fnu4HI GCNGC 4 cut(s) 42, 717, 837, 840
FokI GGATG 1 cut(s) 829
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 4 cut(s) 42, 717, 837, 840
FspBI CTAG 2 cut(s) 449, 602
GluI GCNGC 4 cut(s) 42, 717, 837, 840
GsaI CCCAGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 709
HaeIII GGCC 2 cut(s) 471, 700
HapII CCGG 2 cut(s) 338, 593
Hin1II CATG 5 cut(s) 49, 421, 583, 774, 1001
HindIII AAGCTT 1 cut(s) 234
HpaII CCGG 2 cut(s) 338, 593
HphI GGTGA 2 cut(s) 294, 875
Hpy166II GTNNAC 2 cut(s) 302, 659
Hpy188I TCNGA 3 cut(s) 371, 496, 570
Hpy188III TCNNGA 2 cut(s) 580, 965
Hpy8I GTNNAC 2 cut(s) 302, 659
HpyAV CCTTC 2 cut(s) 551, 575
HpyCH4III ACNGT 6 cut(s) 55, 157, 299, 478, 532, 615
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 4 cut(s) 41, 278, 842, 909
HpyF10VI GCNNNNNNNGC 2 cut(s) 716, 981
HpyF3I CTNAG 3 cut(s) 383, 597, 695
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 5 cut(s) 49, 421, 583, 774, 1001
KpnI GGTACC 1 cut(s) 541
Kzo9I GATC 2 cut(s) 252, 961
LmnI GCTCC 2 cut(s) 774, 893
Lsp1109I GCAGC 4 cut(s) 53, 703, 823, 826
LweI GCATC 2 cut(s) 405, 851
MabI ACCWGGT 1 cut(s) 554
MaeI CTAG 2 cut(s) 449, 602
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 4 cut(s) 17, 49, 116, 993
MalI GATC 2 cut(s) 254, 963
MboI GATC 2 cut(s) 252, 961
MboII GAAGA 8 cut(s) 152, 164, 215, 358, 595, 802, 959, 962
MfeI CAATTG 1 cut(s) 825
MhlI GDGCHC 4 cut(s) 65, 448, 738, 758
MluCI AATT 2 cut(s) 636, 825
MmeI TCCRAC 1 cut(s) 871
MnlI CCTC 9 cut(s) 3, 176, 180, 216, 251, 341, 461, 869, 983
Mph1103I ATGCAT 1 cut(s) 911
MseI TTAA 4 cut(s) 77, 231, 680, 765
MslI CAYNNNNRTG 1 cut(s) 652
MspI CCGG 2 cut(s) 338, 593
MspR9I CCNGG 4 cut(s) 556, 593, 688, 702
MunI CAATTG 1 cut(s) 825
MvaI CCWGG 3 cut(s) 556, 688, 702
MwoI GCNNNNNNNGC 2 cut(s) 716, 981
NciI CCSGG 1 cut(s) 593
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 252, 961
NlaIII CATG 5 cut(s) 49, 421, 583, 774, 1001
NlaIV GGNNCC 3 cut(s) 539, 776, 1011
NmeAIII GCCGAG 1 cut(s) 858
NsiI ATGCAT 1 cut(s) 911
OliI CACNNNNGTG 1 cut(s) 652
PagI TCATGA 1 cut(s) 579
PceI AGGCCT 1 cut(s) 700
PkrI GCNGC 4 cut(s) 43, 718, 838, 841
PpuMI RGGWCCY 1 cut(s) 791
Psp1406I AACGTT 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 791
Psp6I CCWGG 3 cut(s) 554, 686, 700
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 3 cut(s) 554, 686, 700
PspN4I GGNNCC 3 cut(s) 539, 776, 1011
PspPI GGNCC 2 cut(s) 470, 791
PspPPI RGGWCCY 1 cut(s) 791
PsrI GAACNNNNNNTAC 2 cut(s) 530, 562
RsaI GTAC 3 cut(s) 424, 539, 660
RsaNI GTAC 3 cut(s) 423, 538, 659
RseI CAYNNNNRTG 1 cut(s) 652
SaqAI TTAA 4 cut(s) 77, 231, 680, 765
SatI GCNGC 4 cut(s) 42, 717, 837, 840
Sau3AI GATC 2 cut(s) 252, 961
Sau96I GGNCC 2 cut(s) 470, 791
ScrFI CCNGG 4 cut(s) 556, 593, 688, 702
SduI GDGCHC 4 cut(s) 65, 448, 738, 758
SexAI ACCWGGT 1 cut(s) 554
SfaNI GCATC 2 cut(s) 405, 851
SfcI CTRYAG 2 cut(s) 426, 463
SinI GGWCC 1 cut(s) 791
SmiMI CAYNNNNRTG 1 cut(s) 652
SmlI CTYRAG 2 cut(s) 751, 985
SmoI CTYRAG 2 cut(s) 751, 985
Sse9I AATT 2 cut(s) 636, 825
SseBI AGGCCT 1 cut(s) 700
SspMI CTAG 2 cut(s) 449, 602
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 4 cut(s) 554, 591, 686, 700
StyI CCWWGG 2 cut(s) 45, 244
TaaI ACNGT 6 cut(s) 55, 157, 299, 478, 532, 615
TaiI ACGT 1 cut(s) 72
TasI AATT 2 cut(s) 636, 825
TatI WGTACW 1 cut(s) 658
Tru1I TTAA 4 cut(s) 77, 231, 680, 765
Tru9I TTAA 4 cut(s) 77, 231, 680, 765
TscAI CASTG 6 cut(s) 60, 160, 618, 657, 853, 889
TseI GCWGC 4 cut(s) 41, 716, 836, 839
TspDTI ATGAA 5 cut(s) 118, 169, 340, 596, 971
TspRI CASTG 6 cut(s) 60, 160, 618, 657, 853, 889
VpaK11BI GGWCC 1 cut(s) 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 2 cut(s) 449, 602
Zsp2I ATGCAT 1 cut(s) 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.