RLG00000024182
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001369
Physical Location & Seq
Reverse (-)
32829068 .. 32832441
3374 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024182

Sequence Viewer

Length: 1026 bp
ATGCCTCACCACCTTTCTTCCTCTTCCTCATTTTCTTCACTAGTAAACATGGGTTTCTTGAAAAGATCCATCGCCTTGAACAAAAATAGAACCAGCACCATTCACTCTGCTCTCAAGTCTCCTTCAGTTCTTCACTTCCCAAAACAGCCTTACCATTCGCAGGTTATTACTAAAGAGGCCACTATAACACCTGCAAACTGGAATTTGCTTCAGAGAACTGCCTCCACCTGCTTGGACATGTTGGAAGGACTCTTCCACCCTGCGCGTGCAGGGCACGACCCTCACGACCAACTAGCACGTCAAGAAGGGACAAGGTCTCGGTCGAGAAACTGCGCCAAGGGTTCAAAAAGGTCGTCAGCCGACCTCGCCGTGGTTCTGCTGCAGCCAACGGGACTCGCACAACTATTCTTTATGGAGCCTAGGCAAACCAAGCTCTGCGCCAAGGTGGAAACTCACTCCAACCCTAACAAGTACTCCAAGAACATTTTCCGGGCTTTCGTGAAGCACGTTGATCTGAGCGTCGTACGGTGCGTCGTAATAGGGAGTCAGAGAAACGCGAAGGACAAGTTCCGTCGTTTTATGATTCCGGAGGCGAATAGGTTGAAGGTCAAGTCAGTAGAGAACAACAAGCAACGCATTGTCGTTTTTAACACGAGGAGCAAAGGGAACGACGGGAACAGTAGTTTGCATGACAGTACCTTGATGAACTTGATCAGAGACACCAAGGCTGTGACGGAGATCAAGGCTTTTAAGGAGTTTTCAGATTTGCTAACACCGGATTTGGACCGAGTGTGTTATGGTACCAAGAATGTGGAGATGGCGCATGTGTTAAAGGCCATTGAAACGCTCTTGATTACCGATGACTTGTATGAGAGTGTAGAGATTGAGACGAGGTACAAGTATATAAGAGGGAAGGCGTTTGTGTTTTCGTCCAAGCATGTATCTGGAGAGGAGCTAGCTAATCTCAGCGGCATTGCGGCAATTCTGAGGTTTCCACCGCCTGGTCTTGATGAAATGGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

342

Amino Acids

38.25

Weight (kDa)

9.76

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eRF1_3 PF03465 244 - 332 7.8e-17 eRF1 domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 199, 236
Acc36I ACCTGC 3 cut(s) 151, 199, 236
Acc65I GGTACC 1 cut(s) 800
AccB1I GGYRCC 1 cut(s) 800
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccII CGCG 2 cut(s) 265, 557
AccIII TCCGGA 1 cut(s) 586
AciI CCGC 3 cut(s) 969, 977, 998
AclWI GGATC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 202
AcuI CTGAAG 2 cut(s) 108, 194
AfaI GTAC 5 cut(s) 473, 525, 697, 802, 896
AfiI CCNNNNNNNGG 3 cut(s) 160, 370, 1001
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 5 cut(s) 61, 79, 345, 604, 842
AhlI ACTAGT 1 cut(s) 40
AjiI CACGTC 1 cut(s) 299
AjnI CCWGG 1 cut(s) 1000
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 3 cut(s) 433, 955, 959
AluI AGCT 3 cut(s) 433, 955, 959
Alw26I GTCTC 4 cut(s) 123, 321, 711, 881
AlwI GGATC 1 cut(s) 60
Aor13HI TCCGGA 1 cut(s) 586
AoxI GGCC 2 cut(s) 177, 834
ApeKI GCWGC 2 cut(s) 379, 382
ApoI RAATTY 1 cut(s) 202
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
Asp700I GAANNNNTTC 1 cut(s) 485
Asp718I GGTACC 1 cut(s) 800
AspA2I CCTAGG 1 cut(s) 419
AspLEI GCGC 4 cut(s) 265, 335, 440, 823
AspS9I GGNCC 1 cut(s) 784
AsuC2I CCSGG 1 cut(s) 491
AsuNHI GCTAGC 1 cut(s) 955
AvaII GGWCC 1 cut(s) 784
AvrII CCTAGG 1 cut(s) 419
BaeGI GKGCMC 1 cut(s) 276
BanI GGYRCC 1 cut(s) 800
BauI CACGAG 1 cut(s) 652
BbvI GCAGC 2 cut(s) 366, 394
BccI CCATC 2 cut(s) 77, 811
BceAI ACGGC 1 cut(s) 353
BciT130I CCWGG 1 cut(s) 1002
BclI TGATCA 1 cut(s) 711
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 4 cut(s) 123, 321, 711, 881
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 293, 420, 956
BfmI CTRYAG 1 cut(s) 380
BfuAI ACCTGC 3 cut(s) 151, 199, 236
BisI GCNGC 4 cut(s) 380, 383, 970, 978
BlnI CCTAGG 1 cut(s) 419
BlsI GCNGC 4 cut(s) 381, 384, 971, 979
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 2 cut(s) 491, 1002
Bme18I GGWCC 1 cut(s) 784
BmgBI CACGTC 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 784
BmiI GGNNCC 2 cut(s) 417, 802
BmrFI CCNGG 2 cut(s) 491, 1002
BmtI GCTAGC 1 cut(s) 959
BpmI CTGGAG 1 cut(s) 966
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 5 cut(s) 336, 369, 419, 441, 723
BsaWI WCCGGW 2 cut(s) 586, 775
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 370, 1001
Bse1I ACTGG 1 cut(s) 203
Bse3DI GCAATG 1 cut(s) 972
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 1 cut(s) 1002
BseDI CCNNGG 5 cut(s) 336, 369, 419, 441, 723
BseLI CCNNNNNNNGG 3 cut(s) 160, 370, 1001
BseMI GCAATG 1 cut(s) 972
BseMII CTCAG 3 cut(s) 506, 977, 979
BseNI ACTGG 1 cut(s) 203
BseRI GAGGAG 2 cut(s) 670, 965
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 366, 394
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 265, 557
Bsh1285I CGRYCG 1 cut(s) 323
BshFI GGCC 2 cut(s) 179, 836
BshNI GGYRCC 1 cut(s) 800
BsiEI CGRYCG 1 cut(s) 323
BsiSI CCGG 3 cut(s) 490, 587, 776
BsiWI CGTACG 1 cut(s) 523
BslFI GGGAC 2 cut(s) 322, 405
BslI CCNNNNNNNGG 3 cut(s) 160, 370, 1001
BsmAI GTCTC 4 cut(s) 123, 321, 711, 881
BsmBI CGTCTC 1 cut(s) 881
BsmFI GGGAC 2 cut(s) 322, 405
BsnI GGCC 2 cut(s) 179, 836
Bso31I GGTCTC 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 276
Bsp13I TCCGGA 1 cut(s) 586
Bsp143I GATC 4 cut(s) 65, 511, 711, 738
BspACI CCGC 3 cut(s) 969, 977, 998
BspANI GGCC 2 cut(s) 179, 836
BspCNI CTCAG 3 cut(s) 507, 978, 978
BspEI TCCGGA 1 cut(s) 586
BspFNI CGCG 2 cut(s) 265, 557
BspLI GGNNCC 2 cut(s) 417, 802
BspMAI CTGCAG 1 cut(s) 384
BspMI ACCTGC 3 cut(s) 151, 199, 236
BspOI GCTAGC 1 cut(s) 959
BspPI GGATC 1 cut(s) 60
BspT107I GGYRCC 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 972
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 5 cut(s) 336, 369, 419, 441, 723
BssMI GATC 4 cut(s) 65, 511, 711, 738
BssSI CACGAG 1 cut(s) 652
BssT1I CCWWGG 4 cut(s) 336, 419, 441, 723
Bst2BI CACGAG 1 cut(s) 652
Bst2UI CCWGG 1 cut(s) 1002
Bst4CI ACNGT 3 cut(s) 528, 680, 695
Bst6I CTCTTC 2 cut(s) 28, 257
BstC8I GCNNGC 2 cut(s) 267, 957
BstDEI CTNAG 3 cut(s) 515, 965, 986
BstDSI CCRYGG 1 cut(s) 369
BstFNI CGCG 2 cut(s) 265, 557
BstHHI GCGC 4 cut(s) 265, 335, 440, 823
BstKTI GATC 4 cut(s) 68, 514, 714, 741
BstMAI GTCTC 4 cut(s) 123, 321, 711, 881
BstMBI GATC 4 cut(s) 65, 511, 711, 738
BstMCI CGRYCG 1 cut(s) 323
BstMWI GCNNNNNNNGC 3 cut(s) 271, 365, 430
BstNI CCWGG 1 cut(s) 1002
BstNSI RCATGY 3 cut(s) 241, 827, 941
BstSCI CCNGG 2 cut(s) 489, 1000
BstSFI CTRYAG 1 cut(s) 380
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 2 cut(s) 265, 557
BstV1I GCAGC 2 cut(s) 366, 394
BstX2I RGATCY 1 cut(s) 65
BstXI CCANNNNNNTGG 2 cut(s) 232, 811
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 2 cut(s) 179, 836
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 55
BtrI CACGTC 1 cut(s) 299
BveI ACCTGC 3 cut(s) 151, 199, 236
Cac8I GCNNGC 2 cut(s) 267, 957
CfoI GCGC 4 cut(s) 265, 335, 440, 823
Cfr13I GGNCC 1 cut(s) 784
CseI GACGC 2 cut(s) 508, 520
Csp6I GTAC 5 cut(s) 472, 524, 696, 801, 895
CviAII CATG 5 cut(s) 49, 238, 689, 824, 938
CviQI GTAC 5 cut(s) 472, 524, 696, 801, 895
DdeI CTNAG 3 cut(s) 515, 965, 986
DpnI GATC 4 cut(s) 67, 513, 713, 740
DpnII GATC 4 cut(s) 65, 511, 711, 738
Eam1104I CTCTTC 2 cut(s) 28, 257
EarI CTCTTC 2 cut(s) 28, 257
Eco130I CCWWGG 4 cut(s) 336, 419, 441, 723
Eco31I GGTCTC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 2 cut(s) 108, 194
EcoRII CCWGG 1 cut(s) 1000
EcoT14I CCWWGG 4 cut(s) 336, 419, 441, 723
ErhI CCWWGG 4 cut(s) 336, 419, 441, 723
Esp3I CGTCTC 1 cut(s) 881
FaeI CATG 5 cut(s) 52, 241, 692, 827, 941
FaqI GGGAC 2 cut(s) 322, 405
FatI CATG 5 cut(s) 48, 237, 688, 823, 937
FbaI TGATCA 1 cut(s) 711
Fnu4HI GCNGC 4 cut(s) 380, 383, 970, 978
Fsp4HI GCNGC 4 cut(s) 380, 383, 970, 978
FspBI CTAG 4 cut(s) 41, 293, 420, 956
GlaI GCGC 4 cut(s) 264, 334, 439, 822
GluI GCNGC 4 cut(s) 380, 383, 970, 978
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 2 cut(s) 179, 836
HapII CCGG 3 cut(s) 490, 587, 776
HgaI GACGC 2 cut(s) 508, 520
HhaI GCGC 4 cut(s) 265, 335, 440, 823
Hin1II CATG 5 cut(s) 52, 241, 692, 827, 941
Hin6I GCGC 4 cut(s) 263, 333, 438, 821
HinP1I GCGC 4 cut(s) 263, 333, 438, 821
HinfI GANTC 4 cut(s) 249, 393, 544, 583
HpaII CCGG 3 cut(s) 490, 587, 776
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 6 cut(s) 213, 516, 549, 716, 763, 987
Hpy188III TCNNGA 9 cut(s) 58, 284, 302, 324, 499, 587, 850, 945, 1007
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 4 cut(s) 524, 536, 576, 674
HpyAV CCTTC 6 cut(s) 132, 239, 299, 553, 598, 907
HpyCH4III ACNGT 3 cut(s) 528, 680, 695
HpyCH4IV ACGT 2 cut(s) 298, 507
HpyCH4V TGCA 4 cut(s) 194, 269, 382, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 365, 430
HpyF3I CTNAG 3 cut(s) 515, 965, 986
HpySE526I ACGT 2 cut(s) 298, 507
Hsp92II CATG 5 cut(s) 52, 241, 692, 827, 941
HspAI GCGC 4 cut(s) 263, 333, 438, 821
Kpn2I TCCGGA 1 cut(s) 586
KpnI GGTACC 1 cut(s) 804
Ksp22I TGATCA 1 cut(s) 711
Kzo9I GATC 4 cut(s) 65, 511, 711, 738
LmnI GCTCC 3 cut(s) 415, 657, 952
Lsp1109I GCAGC 2 cut(s) 366, 394
MaeI CTAG 4 cut(s) 41, 293, 420, 956
MaeII ACGT 2 cut(s) 298, 507
MaeIII GTNAC 1 cut(s) 730
MalI GATC 4 cut(s) 67, 513, 713, 740
MboI GATC 4 cut(s) 65, 511, 711, 738
MboII GAAGA 5 cut(s) 9, 15, 27, 122, 244
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 202, 981
MlyI GAGTC 3 cut(s) 243, 387, 553
MmeI TCCRAC 2 cut(s) 222, 483
MroI TCCGGA 1 cut(s) 586
MroXI GAANNNNTTC 1 cut(s) 485
MseI TTAA 4 cut(s) 648, 750, 830, 1024
MspA1I CMGCKG 1 cut(s) 969
MspI CCGG 3 cut(s) 490, 587, 776
MspR9I CCNGG 2 cut(s) 491, 1002
MvaI CCWGG 1 cut(s) 1002
MvnI CGCG 2 cut(s) 265, 557
MwoI GCNNNNNNNGC 3 cut(s) 271, 365, 430
NciI CCSGG 1 cut(s) 491
NdeII GATC 4 cut(s) 65, 511, 711, 738
NheI GCTAGC 1 cut(s) 955
NlaIII CATG 5 cut(s) 52, 241, 692, 827, 941
NlaIV GGNNCC 2 cut(s) 417, 802
NmuCI GTSAC 1 cut(s) 730
NspI RCATGY 3 cut(s) 241, 827, 941
PaqCI CACCTGC 2 cut(s) 199, 236
PciI ACATGT 1 cut(s) 237
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 504, 528
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 1 cut(s) 583
Pfl23II CGTACG 1 cut(s) 523
PflFI GACNNNGTC 1 cut(s) 313
PflMI CCANNNNNTGG 1 cut(s) 1001
PkrI GCNGC 4 cut(s) 381, 384, 971, 979
PleI GAGTC 3 cut(s) 243, 387, 552
PpsI GAGTC 3 cut(s) 243, 387, 552
PscI ACATGT 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 1000
PspGI CCWGG 1 cut(s) 1000
PspLI CGTACG 1 cut(s) 523
PspN4I GGNNCC 2 cut(s) 417, 802
PspPI GGNCC 1 cut(s) 784
PstI CTGCAG 1 cut(s) 384
PsuI RGATCY 1 cut(s) 65
PsyI GACNNNGTC 1 cut(s) 313
RsaI GTAC 5 cut(s) 473, 525, 697, 802, 896
RsaNI GTAC 5 cut(s) 472, 524, 696, 801, 895
SaqAI TTAA 4 cut(s) 648, 750, 830, 1024
SatI GCNGC 4 cut(s) 380, 383, 970, 978
Sau3AI GATC 4 cut(s) 65, 511, 711, 738
Sau96I GGNCC 1 cut(s) 784
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 3 cut(s) 243, 387, 553
ScrFI CCNGG 2 cut(s) 491, 1002
SduI GDGCHC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 380
SinI GGWCC 1 cut(s) 784
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 2 cut(s) 202, 981
SsiI CCGC 3 cut(s) 969, 977, 998
SspMI CTAG 4 cut(s) 41, 293, 420, 956
StyD4I CCNGG 2 cut(s) 489, 1000
StyI CCWWGG 4 cut(s) 336, 419, 441, 723
TaaI ACNGT 3 cut(s) 528, 680, 695
TaiI ACGT 2 cut(s) 301, 510
TaqI TCGA 1 cut(s) 323
TaqII GACCGA 2 cut(s) 309, 801
TasI AATT 2 cut(s) 202, 981
TatI WGTACW 1 cut(s) 471
TauI GCSGC 2 cut(s) 972, 980
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 4 cut(s) 648, 750, 830, 1024
Tru9I TTAA 4 cut(s) 648, 750, 830, 1024
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 2 cut(s) 379, 382
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 2 cut(s) 719, 1026
TspGWI ACGGA 2 cut(s) 560, 749
Tth111I GACNNNGTC 1 cut(s) 313
Van91I CCANNNNNTGG 1 cut(s) 1001
VpaK11BI GGWCC 1 cut(s) 784
XapI RAATTY 1 cut(s) 202
XceI RCATGY 3 cut(s) 241, 827, 941
XmaJI CCTAGG 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 4 cut(s) 41, 293, 420, 956
ZrmI AGTACT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.