Rh3BG192700
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001369
Physical Location & Seq
Forward (+)
17062617 .. 17063544
928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG192700.1

Sequence Viewer

Length: 663 bp
ATGAAACTCGTCATAGAAAACAATATCGTTGTGAATCAACGAGGGACGGTCAAGATCCTCCCCGAAGACCTCGACGACGTCTGGTTTGTTTTCAATCTCATCTCCCCCGGTGACGTCATCATCGCCGCCACCACCCGCAGCGTCAAATACAGCTCCCGCATGAAGGCTTTCGTGAAGCACGTTGATCTGAGCGTCGTACGGTGCGTCGTAATAGGGAGTCAGGGAAACGCGAAGGACGAGTTCCGTCGTTTTATGATTGCGGAGGCGAATAGGTTGAAGGTCAAGTCAGTAGAGAACAACAAGCAACGCATTGTCGTTTTTAACACGAGGAGCAAAGGGAAAGACGGGAACAGTAGTTTGCTTGAGGTTTTGCATGACAGTACCGTGATGAACTTGGTCAGAGACACCAAGGCTGTGATGGAGATCAAGGCTTTTAAGGAGTTTTCGGATTTGCCAACAACGGATTTGGACCGAGTGTGTTATGATACCAAGAATGTGGAGATGGCGCATGAGTTGAAGGCCATTGAAACGCTATTGATTACCGATGACTTGTATGAGAGTGTAGAGATTGAGACGAGGTATAAGTACAGAGGGTTTGTGGAGTCGGAGAAAAAGACAGGAGGGAAGGCGTTTGAGTTTTCGTCCGAGCATGTATCCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

220

Amino Acids

25.01

Weight (kDa)

6.92

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pelota_N PF26356 1 - 55 4.6e-11 Pelota N-terminal domain
eRF1_2 PF03464 54 - 129 5.7e-06 eRF1 domain 2
eRF1_3 PF03465 139 - 218 1.5e-09 eRF1 domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 81, 117
AccII CGCG 1 cut(s) 230
AciI CCGC 4 cut(s) 126, 136, 157, 260
AclWI GGATC 1 cut(s) 49
AcyI GRCGYC 2 cut(s) 78, 114
AfaI GTAC 3 cut(s) 198, 382, 587
AfiI CCNNNNNNNGG 1 cut(s) 163
AgsI TTSAA 4 cut(s) 94, 277, 517, 527
AluBI AGCT 2 cut(s) 153, 660
AluI AGCT 2 cut(s) 153, 660
Alw26I GTCTC 2 cut(s) 396, 566
AlwI GGATC 1 cut(s) 49
AoxI GGCC 1 cut(s) 519
ApeKI GCWGC 1 cut(s) 138
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 167
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 122
AvaII GGWCC 1 cut(s) 469
BauI CACGAG 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 150
BccI CCATC 2 cut(s) 412, 496
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 396, 566
BfaI CTAG 1 cut(s) 661
BisI GCNGC 2 cut(s) 126, 139
BlsI GCNGC 2 cut(s) 127, 140
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 469
BmrFI CCNGG 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 422
BsaHI GRCGYC 2 cut(s) 78, 114
BsaJI CCNNGG 2 cut(s) 106, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 106, 408
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 179
BseRI GAGGAG 1 cut(s) 343
BseXI GCAGC 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 1 cut(s) 521
BsiSI CCGG 1 cut(s) 108
BsiWI CGTACG 1 cut(s) 196
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 2 cut(s) 396, 566
BsmBI CGTCTC 1 cut(s) 566
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 1 cut(s) 521
Bsp143I GATC 3 cut(s) 54, 184, 423
BspACI CCGC 4 cut(s) 126, 136, 157, 260
BspANI GGCC 1 cut(s) 521
BspCNI CTCAG 1 cut(s) 180
BspFNI CGCG 1 cut(s) 230
BspPI GGATC 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 106, 408
BssMI GATC 3 cut(s) 54, 184, 423
BssNI GRCGYC 2 cut(s) 78, 114
BssSI CACGAG 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 325
Bst4CI ACNGT 5 cut(s) 49, 201, 353, 380, 385
BstACI GRCGYC 2 cut(s) 78, 114
BstDEI CTNAG 1 cut(s) 188
BstFNI CGCG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 3 cut(s) 57, 187, 426
BstMAI GTCTC 2 cut(s) 396, 566
BstMBI GATC 3 cut(s) 54, 184, 423
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 1 cut(s) 106
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 150
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 1 cut(s) 54
BsuRI GGCC 1 cut(s) 521
BtgZI GCGATG 1 cut(s) 106
CfoI GCGC 1 cut(s) 508
Cfr13I GGNCC 1 cut(s) 469
CseI GACGC 3 cut(s) 130, 181, 193
Csp6I GTAC 3 cut(s) 197, 381, 586
CviAII CATG 4 cut(s) 160, 374, 509, 650
CviJI RGCY 6 cut(s) 153, 167, 413, 431, 521, 660
CviKI_1 RGCY 6 cut(s) 153, 167, 413, 431, 521, 660
CviQI GTAC 3 cut(s) 197, 381, 586
DdeI CTNAG 1 cut(s) 188
DpnI GATC 3 cut(s) 56, 186, 425
DpnII GATC 3 cut(s) 54, 184, 423
Eco130I CCWWGG 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 4 cut(s) 163, 377, 512, 653
FaiI YATR 9 cut(s) 14, 161, 254, 375, 483, 510, 555, 582, 651
FaqI GGGAC 1 cut(s) 58
FatI CATG 4 cut(s) 159, 373, 508, 649
FauI CCCGC 2 cut(s) 143, 164
Fnu4HI GCNGC 2 cut(s) 126, 139
Fsp4HI GCNGC 2 cut(s) 126, 139
FspBI CTAG 1 cut(s) 661
GlaI GCGC 1 cut(s) 507
GluI GCNGC 2 cut(s) 126, 139
HaeIII GGCC 1 cut(s) 521
HapII CCGG 1 cut(s) 108
HgaI GACGC 3 cut(s) 130, 181, 193
HhaI GCGC 1 cut(s) 508
Hin1I GRCGYC 2 cut(s) 78, 114
Hin1II CATG 4 cut(s) 163, 377, 512, 653
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HinfI GANTC 3 cut(s) 34, 217, 602
HpaII CCGG 1 cut(s) 108
HphI GGTGA 1 cut(s) 122
Hpy188I TCNGA 5 cut(s) 189, 401, 448, 607, 646
Hpy188III TCNNGA 2 cut(s) 52, 172
Hpy99I CGWCG 5 cut(s) 77, 80, 197, 209, 249
HpyAV CCTTC 5 cut(s) 157, 226, 271, 511, 619
HpyCH4III ACNGT 5 cut(s) 49, 201, 353, 380, 385
HpyCH4IV ACGT 3 cut(s) 78, 114, 180
HpyCH4V TGCA 1 cut(s) 373
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 3 cut(s) 78, 114, 180
Hsp92I GRCGYC 2 cut(s) 78, 114
Hsp92II CATG 4 cut(s) 163, 377, 512, 653
HspAI GCGC 1 cut(s) 506
Kzo9I GATC 3 cut(s) 54, 184, 423
LmnI GCTCC 2 cut(s) 158, 330
LpnPI CCDG 4 cut(s) 67, 121, 206, 603
Lsp1109I GCAGC 1 cut(s) 150
MaeI CTAG 1 cut(s) 661
MaeII ACGT 3 cut(s) 78, 114, 180
MaeIII GTNAC 1 cut(s) 110
MalI GATC 3 cut(s) 56, 186, 425
MboI GATC 3 cut(s) 54, 184, 423
MboII GAAGA 1 cut(s) 77
MflI RGATCY 1 cut(s) 54
MlyI GAGTC 2 cut(s) 226, 611
MmeI TCCRAC 1 cut(s) 585
MnlI CCTC 9 cut(s) 35, 68, 80, 256, 321, 358, 570, 584, 614
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 2 cut(s) 321, 435
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 230
NciI CCSGG 1 cut(s) 108
NdeII GATC 3 cut(s) 54, 184, 423
NlaIII CATG 4 cut(s) 163, 377, 512, 653
NmuCI GTSAC 1 cut(s) 110
NspI RCATGY 1 cut(s) 653
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 201, 234
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 1 cut(s) 34
Pfl23II CGTACG 1 cut(s) 196
PflFI GACNNNGTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 127, 140
PleI GAGTC 2 cut(s) 225, 610
PpsI GAGTC 2 cut(s) 225, 610
PspLI CGTACG 1 cut(s) 196
PspPI GGNCC 1 cut(s) 469
PsuI RGATCY 1 cut(s) 54
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 3 cut(s) 198, 382, 587
RsaNI GTAC 3 cut(s) 197, 381, 586
SaqAI TTAA 2 cut(s) 321, 435
SatI GCNGC 2 cut(s) 126, 139
Sau3AI GATC 3 cut(s) 54, 184, 423
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 2 cut(s) 226, 611
ScrFI CCNGG 1 cut(s) 108
SinI GGWCC 1 cut(s) 469
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
SsiI CCGC 4 cut(s) 126, 136, 157, 260
SspMI CTAG 1 cut(s) 661
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 5 cut(s) 49, 201, 353, 380, 385
TaiI ACGT 3 cut(s) 81, 117, 183
TaqI TCGA 1 cut(s) 72
TaqII GACCGA 1 cut(s) 486
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 2 cut(s) 321, 435
Tru9I TTAA 2 cut(s) 321, 435
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 138
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 3 cut(s) 17, 176, 404
TspGWI ACGGA 2 cut(s) 233, 476
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 469
XceI RCATGY 1 cut(s) 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 1 cut(s) 661
ZraI GACGTC 2 cut(s) 79, 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.