FvH4_6g17270
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001369
Physical Location & Seq
Forward (+)
11204416 .. 11205548
1133 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17270.t1

Sequence Viewer

Length: 1092 bp
ATGAAGCTCATCATAGAAAACAATATCGTCATGAATCAACGAGACACGGTCAAGATCCTCCCGGAAGAACCCGACGACGTCTGGTTCGTCTACAACCTCATCTCCCCCGGTGACGTCATCATCGCCACCACCACCCGCAACGTCAAATACAGCTCCCGGGTGAGGCTCAGCCTCGAAATCTCGATCACCGGCGTCAACTACCACAGGGACTCGTCCACCCTGCGCGTGCATGGCACGACCCTCAACACCAACCAGCACGTCAAGAAGGGTTTGTTCCACACCGTCGAGATCGAGCTCAACAAAGAGTTCGAGATTTACAAGGAAGTCTGGGACAAACTCTCAATCGAGAAACTCCGGCTAGGGTTCAAAAACGCAGCCGGAGCTGACCTCGCCGTGGTTCTTCTGCAGCCGACGGGGTTCGCCCAAGTGTACTTGGTGGTTGCTAGACAGACCAAGCTGTGCGCTAGGGTCGAGACTCACTCAAACCCTAACAAGTACTTCGAGAACATTTTCCGGGCTTTCGTCAAGCACGTCGACCGGAGCGTCGTACGGTGCGTCGTGATAGGGAGTGAGGGAAACGCCAAGGACGAGTTCCGTCGATTCATGATAGCGGAGGCGAATAGGTTGAAGGTGAAGTCAGTAGAGAACAACAAGCAACTCATTGTGGTGTTCAACACGAGGAACAAAGGGAGAGACGGCAACTTTAGCTTGCATGAGGCTTTGCATGATGGTACAGTGATGAACTTGATCAAGGACACCAAGGCTGTGATGGAGATCAAGGCGTTTAAGGAGTTTTCGGATTTGCTAACGACGGATTTGGATCGAGTGTGTTATGGTGCCAAGAATGTGGAGATGGCGCACGAGTTGAAGGCCATTGAGACGCTCTTGATTACCGATGATTGGTTTGTGGAGTCGGTGAAGAAGGCAGGAGGGAAGGCGTTTGTGTTTTCGTCGAAGCATGTTTCAGGCGAGGAGCTGGCTAATCTGACAGGCATTGCTGCGATTTTGAGGTTTCCACTGATCTTGATGAAATGGTTTCTTAACATGAATGTTCATAGTGCTGTATATGTAGGTTCAGAATGTTTAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

364

Amino Acids

41.19

Weight (kDa)

8.69

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pelota_N PF26356 1 - 119 9.6e-35 Pelota N-terminal domain
eRF1_2 PF03464 159 - 252 1.4e-07 eRF1 domain 2
eRF1_3 PF03465 256 - 340 2.7e-17 eRF1 domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 542
AatII GACGTC 2 cut(s) 81, 117
AccB1I GGYRCC 1 cut(s) 836
AccI GTMKAC 2 cut(s) 90, 534
AccII CGCG 1 cut(s) 225
AciI CCGC 2 cut(s) 136, 611
AclWI GGATC 2 cut(s) 49, 828
AcyI GRCGYC 3 cut(s) 78, 114, 192
AfaI GTAC 4 cut(s) 431, 497, 549, 733
AfiI CCNNNNNNNGG 3 cut(s) 162, 394, 900
AgsI TTSAA 4 cut(s) 367, 628, 673, 868
AjiI CACGTC 2 cut(s) 259, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 7 cut(s) 7, 153, 295, 383, 457, 708, 976
AluI AGCT 7 cut(s) 7, 153, 295, 383, 457, 708, 976
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 4 cut(s) 36, 467, 687, 872
AlwI GGATC 2 cut(s) 49, 828
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 1 cut(s) 870
ApeKI GCWGC 3 cut(s) 374, 406, 998
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 1 cut(s) 509
AspLEI GCGC 3 cut(s) 225, 464, 859
AsuC2I CCSGG 5 cut(s) 62, 108, 157, 158, 515
AsuHPI GGTGA 5 cut(s) 122, 172, 178, 643, 928
AvaI CYCGRG 1 cut(s) 156
BanI GGYRCC 1 cut(s) 836
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 2 cut(s) 676, 860
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 386, 418, 985
BccI CCATC 3 cut(s) 722, 763, 847
BceAI ACGGC 2 cut(s) 377, 712
BclI TGATCA 1 cut(s) 747
BcnI CCSGG 5 cut(s) 62, 108, 157, 158, 515
BcoDI GTCTC 4 cut(s) 36, 467, 687, 872
BfaI CTAG 3 cut(s) 359, 444, 465
BfmI CTRYAG 1 cut(s) 404
BisI GCNGC 3 cut(s) 375, 407, 999
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 3 cut(s) 376, 408, 1000
BmcAI AGTACT 1 cut(s) 497
Bme1390I CCNGG 5 cut(s) 62, 108, 157, 158, 515
BmeT110I CYCGRG 1 cut(s) 156
BmgBI CACGTC 2 cut(s) 259, 532
BmiI GGNNCC 1 cut(s) 838
BmrFI CCNGG 5 cut(s) 62, 108, 157, 158, 515
BplI GAGNNNNNCTC 4 cut(s) 372, 404, 464, 496
Bpu1102I GCTNAGC 1 cut(s) 167
BpuMI CCSGG 5 cut(s) 62, 108, 157, 158, 515
BsaBI GATNNNNATC 2 cut(s) 773, 819
BsaHI GRCGYC 3 cut(s) 78, 114, 192
BsaJI CCNNGG 5 cut(s) 106, 156, 393, 582, 759
BsaWI WCCGGW 1 cut(s) 537
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 394, 900
Bse118I RCCGGY 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 993
Bse8I GATNNNNATC 2 cut(s) 773, 819
BseDI CCNNGG 5 cut(s) 106, 156, 393, 582, 759
BseJI GATNNNNATC 2 cut(s) 773, 819
BseLI CCNNNNNNNGG 3 cut(s) 162, 394, 900
BseMI GCAATG 1 cut(s) 993
BseMII CTCAG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 986
BseXI GCAGC 3 cut(s) 386, 418, 985
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 538
BshFI GGCC 1 cut(s) 872
BshNI GGYRCC 1 cut(s) 836
BsiEI CGRYCG 1 cut(s) 538
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 8 cut(s) 62, 108, 157, 189, 355, 378, 514, 538
BsiWI CGTACG 1 cut(s) 547
BslFI GGGAC 2 cut(s) 221, 344
BslI CCNNNNNNNGG 3 cut(s) 162, 394, 900
BsmAI GTCTC 4 cut(s) 36, 467, 687, 872
BsmBI CGTCTC 2 cut(s) 687, 872
BsmFI GGGAC 2 cut(s) 221, 344
BsnI GGCC 1 cut(s) 872
BsoBI CYCGRG 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 2 cut(s) 136, 611
BspANI GGCC 1 cut(s) 872
BspCNI CTCAG 1 cut(s) 180
BspFNI CGCG 1 cut(s) 225
BspHI TCATGA 2 cut(s) 30, 603
BspLI GGNNCC 1 cut(s) 838
BspMAI CTGCAG 1 cut(s) 408
BspPI GGATC 2 cut(s) 49, 828
BspT107I GGYRCC 1 cut(s) 836
BsrDI GCAATG 1 cut(s) 993
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 5 cut(s) 106, 156, 393, 582, 759
BssMI GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
BssNI GRCGYC 3 cut(s) 78, 114, 192
BssSI CACGAG 2 cut(s) 676, 860
BssT1I CCWWGG 2 cut(s) 582, 759
Bst2BI CACGAG 2 cut(s) 676, 860
Bst4CI ACNGT 4 cut(s) 49, 283, 552, 736
BstACI GRCGYC 3 cut(s) 78, 114, 192
BstC8I GCNNGC 3 cut(s) 227, 710, 978
BstDEI CTNAG 1 cut(s) 167
BstDSI CCRYGG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 3 cut(s) 225, 464, 859
BstKTI GATC 7 cut(s) 57, 186, 291, 750, 777, 823, 1023
BstMAI GTCTC 4 cut(s) 36, 467, 687, 872
BstMBI GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
BstMCI CGRYCG 1 cut(s) 538
BstMWI GCNNNNNNNGC 4 cut(s) 231, 380, 389, 705
BstNSI RCATGY 1 cut(s) 962
BstSCI CCNGG 5 cut(s) 60, 106, 155, 156, 513
BstSFI CTRYAG 1 cut(s) 404
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 386, 418, 985
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 847
BstYI RGATCY 1 cut(s) 54
BsuRI GGCC 1 cut(s) 872
BtgI CCRYGG 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 106
BtrI CACGTC 2 cut(s) 259, 532
BtsIMutI CAGTG 2 cut(s) 741, 1016
Cac8I GCNNGC 3 cut(s) 227, 710, 978
CciI TCATGA 2 cut(s) 30, 603
CfoI GCGC 3 cut(s) 225, 464, 859
Cfr10I RCCGGY 1 cut(s) 188
Cfr9I CCCGGG 1 cut(s) 156
CseI GACGC 4 cut(s) 181, 532, 544, 889
Csp6I GTAC 4 cut(s) 430, 496, 548, 732
CspCI CAANNNNNGTGG 2 cut(s) 1005, 1040
CviAII CATG 7 cut(s) 31, 230, 604, 713, 725, 959, 1045
CviQI GTAC 4 cut(s) 430, 496, 548, 732
DdeI CTNAG 1 cut(s) 167
DpnI GATC 7 cut(s) 56, 185, 290, 749, 776, 822, 1022
DpnII GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
DrdI GACNNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 542
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 2 cut(s) 582, 759
Eco24I GRGCYC 1 cut(s) 297
Eco53kI GAGCTC 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 156
EcoICRI GAGCTC 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 582, 759
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 2 cut(s) 582, 759
Esp3I CGTCTC 2 cut(s) 687, 872
FaeI CATG 7 cut(s) 34, 233, 607, 716, 728, 962, 1048
FaqI GGGAC 2 cut(s) 221, 344
FatI CATG 7 cut(s) 30, 229, 603, 712, 724, 958, 1044
FauI CCCGC 1 cut(s) 143
FbaI TGATCA 1 cut(s) 747
FblI GTMKAC 2 cut(s) 90, 534
Fnu4HI GCNGC 3 cut(s) 375, 407, 999
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 3 cut(s) 375, 407, 999
FspBI CTAG 3 cut(s) 359, 444, 465
GlaI GCGC 3 cut(s) 224, 463, 858
GluI GCNGC 3 cut(s) 375, 407, 999
HaeIII GGCC 1 cut(s) 872
HapII CCGG 8 cut(s) 62, 108, 157, 189, 355, 378, 514, 538
HgaI GACGC 4 cut(s) 181, 532, 544, 889
HhaI GCGC 3 cut(s) 225, 464, 859
Hin1I GRCGYC 3 cut(s) 78, 114, 192
Hin1II CATG 7 cut(s) 34, 233, 607, 716, 728, 962, 1048
Hin6I GCGC 3 cut(s) 223, 462, 857
HinP1I GCGC 3 cut(s) 223, 462, 857
HincII GTYRAC 2 cut(s) 196, 535
HindII GTYRAC 2 cut(s) 196, 535
HinfI GANTC 5 cut(s) 34, 209, 475, 600, 911
HpaII CCGG 8 cut(s) 62, 108, 157, 189, 355, 378, 514, 538
HphI GGTGA 5 cut(s) 122, 172, 178, 643, 928
Hpy166II GTNNAC 5 cut(s) 91, 196, 216, 430, 535
Hpy188I TCNGA 3 cut(s) 799, 987, 1078
Hpy8I GTNNAC 5 cut(s) 91, 196, 216, 430, 535
HpyAV CCTTC 5 cut(s) 259, 622, 862, 916, 928
HpyCH4III ACNGT 4 cut(s) 49, 283, 552, 736
HpyCH4IV ACGT 5 cut(s) 78, 114, 141, 258, 531
HpyCH4V TGCA 4 cut(s) 229, 406, 712, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 380, 389, 705
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 5 cut(s) 78, 114, 141, 258, 531
Hsp92I GRCGYC 3 cut(s) 78, 114, 192
Hsp92II CATG 7 cut(s) 34, 233, 607, 716, 728, 962, 1048
HspAI GCGC 3 cut(s) 223, 462, 857
Ksp22I TGATCA 1 cut(s) 747
Kzo9I GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
LmnI GCTCC 4 cut(s) 158, 380, 540, 973
Lsp1109I GCAGC 3 cut(s) 386, 418, 985
MaeI CTAG 3 cut(s) 359, 444, 465
MaeII ACGT 5 cut(s) 78, 114, 141, 258, 531
MaeIII GTNAC 1 cut(s) 110
MalI GATC 7 cut(s) 56, 185, 290, 749, 776, 822, 1022
MboI GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
MboII GAAGA 3 cut(s) 77, 392, 931
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 297
MlyI GAGTC 3 cut(s) 203, 469, 920
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 2 cut(s) 786, 1041
MslI CAYNNNNRTG 1 cut(s) 665
MspI CCGG 8 cut(s) 62, 108, 157, 189, 355, 378, 514, 538
MspR9I CCNGG 5 cut(s) 62, 108, 157, 158, 515
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 4 cut(s) 231, 380, 389, 705
NciI CCSGG 5 cut(s) 62, 108, 157, 158, 515
NdeII GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
NlaIII CATG 7 cut(s) 34, 233, 607, 716, 728, 962, 1048
NlaIV GGNNCC 1 cut(s) 838
NmuCI GTSAC 1 cut(s) 110
NspI RCATGY 1 cut(s) 962
PagI TCATGA 2 cut(s) 30, 603
PcsI WCGNNNNNNNCGW 5 cut(s) 84, 528, 540, 552, 585
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 2 cut(s) 34, 600
Pfl23II CGTACG 1 cut(s) 547
PflFI GACNNNGTC 2 cut(s) 47, 77
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 3 cut(s) 376, 408, 1000
PleI GAGTC 3 cut(s) 203, 469, 919
PpsI GAGTC 3 cut(s) 203, 469, 919
Psp124BI GAGCTC 1 cut(s) 297
PspLI CGTACG 1 cut(s) 547
PspN4I GGNNCC 1 cut(s) 838
PstI CTGCAG 1 cut(s) 408
PsuI RGATCY 1 cut(s) 54
PsyI GACNNNGTC 2 cut(s) 47, 77
RsaI GTAC 4 cut(s) 431, 497, 549, 733
RsaNI GTAC 4 cut(s) 430, 496, 548, 732
RseI CAYNNNNRTG 1 cut(s) 665
SacI GAGCTC 1 cut(s) 297
SalI GTCGAC 1 cut(s) 533
SaqAI TTAA 2 cut(s) 786, 1041
SatI GCNGC 3 cut(s) 375, 407, 999
Sau3AI GATC 7 cut(s) 54, 183, 288, 747, 774, 820, 1020
ScaI AGTACT 1 cut(s) 497
SchI GAGTC 3 cut(s) 203, 469, 920
ScrFI CCNGG 5 cut(s) 62, 108, 157, 158, 515
SduI GDGCHC 1 cut(s) 297
SfcI CTRYAG 1 cut(s) 404
SgrAI CRCCGGYG 1 cut(s) 188
SmaI CCCGGG 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 665
SsiI CCGC 2 cut(s) 136, 611
SspMI CTAG 3 cut(s) 359, 444, 465
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 5 cut(s) 60, 106, 155, 156, 513
StyI CCWWGG 2 cut(s) 582, 759
TaaI ACNGT 4 cut(s) 49, 283, 552, 736
TaiI ACGT 5 cut(s) 81, 117, 144, 261, 534
TatI WGTACW 2 cut(s) 429, 495
TfiI GAWTC 2 cut(s) 34, 600
Tru1I TTAA 2 cut(s) 786, 1041
Tru9I TTAA 2 cut(s) 786, 1041
TscAI CASTG 2 cut(s) 741, 1023
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 3 cut(s) 374, 406, 998
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 7 cut(s) 17, 47, 592, 755, 1043, 1043, 1061
TspGWI ACGGA 2 cut(s) 584, 827
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 2 cut(s) 741, 1023
Tth111I GACNNNGTC 2 cut(s) 47, 77
XceI RCATGY 1 cut(s) 962
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XmaI CCCGGG 1 cut(s) 156
XmiI GTMKAC 2 cut(s) 90, 534
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 3 cut(s) 359, 444, 465
ZraI GACGTC 2 cut(s) 79, 115
ZrmI AGTACT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.