Rw3G015750
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001369
Physical Location & Seq
Forward (+)
16308497 .. 16313486
4990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015750.1

Sequence Viewer

Length: 756 bp
ATGAAACTCGTCACAGAAAACAATATCGTTGTGAATCAACGAGGGACGGTCAAGATCCTCCCCGAAGACCTCGACGACGTCTGGTTTGTTTTCAACCTCATCTCCCCTAGTGACGTCATCATCGCTGCTGCCACCCGCAACGACAAATACAGCTCCCGCATGAAGGTCTCGGTCGAGAAACTGCGCCAAGGGTTCAAAAAGGTTGTCGGAGCCGACCTCGTCGTGGTTCTGCTACAGCCAGCGAGACTCGCACAACTGTTCTTGGTGGAAACTCACTCCAACCCTAACAAGTACTTCGAGAACATTTTCCAGGCTTTCATGAAGCACGTTGATCTGAGCGTCGTACGGTGCGTCGTAATAGGGAGTCAGGGAAACGCGAAGGACGAGTTCCGTCGTTTTATGATTGCGGAGGCGAATAGGTTGAAGGTCAAGTCAGTAGAGAACAACAAGCAACGCATTGTCGTTTTTAACACGAGGAACAAAGGGAAAGACGGGAACAGTAGTTCGCATGAGGTTTTGCATGACAGTACCGTGATGAACTTGATCAGAGACACCAAGGCTGTGATGGAGATCAAGGCTTTTAAGGAGTTTTCGGATTTGCTAACAACGGATTTGGACCGAGTGTGTTATGGTACCAAGAATGTGGAGATGGCGCATGAGTTGAAGGCCATTGAAACGCTCTTGATTACCGATGACTTGTATGAGAGTGTAGAGATTGAGACTTGGTATACGTACAGAGGGTTTGTGAGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

251

Amino Acids

28.72

Weight (kDa)

8.41

Isoelectric Point (pI)

28.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pelota_N PF26356 1 - 56 5.9e-09 Pelota N-terminal domain
eRF1_2 PF03464 82 - 179 1.3e-07 eRF1 domain 2
eRF1_3 PF03465 188 - 237 1.9e-09 eRF1 domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 81, 117
Acc65I GGTACC 1 cut(s) 632
AccB1I GGYRCC 1 cut(s) 632
AccI GTMKAC 1 cut(s) 728
AccII CGCG 1 cut(s) 377
AciI CCGC 3 cut(s) 136, 157, 407
AclWI GGATC 1 cut(s) 49
AcyI GRCGYC 2 cut(s) 78, 114
AfaI GTAC 5 cut(s) 293, 345, 529, 634, 734
AfiI CCNNNNNNNGG 2 cut(s) 163, 223
AgsI TTSAA 5 cut(s) 94, 196, 424, 664, 674
AjnI CCWGG 1 cut(s) 309
AloI GAACNNNNNNTCC 2 cut(s) 487, 519
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw26I GTCTC 4 cut(s) 172, 238, 543, 713
AlwI GGATC 1 cut(s) 49
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 2 cut(s) 125, 128
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 189, 221
Asp700I GAANNNNTTC 1 cut(s) 305
Asp718I GGTACC 1 cut(s) 632
AspLEI GCGC 2 cut(s) 186, 655
AspS9I GGNCC 1 cut(s) 616
AvaII GGWCC 1 cut(s) 616
BanI GGYRCC 1 cut(s) 632
BauI CACGAG 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 112, 115
BccI CCATC 2 cut(s) 559, 643
BciT130I CCWGG 1 cut(s) 311
BclI TGATCA 1 cut(s) 543
BcoDI GTCTC 4 cut(s) 172, 238, 543, 713
BfaI CTAG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 233
BisI GCNGC 2 cut(s) 126, 129
BlsI GCNGC 2 cut(s) 127, 130
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 616
BmgT120I GGNCC 1 cut(s) 616
BmiI GGNNCC 2 cut(s) 211, 634
BmrFI CCNGG 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 201, 233
BsaAI YACGTR 1 cut(s) 732
BsaBI GATNNNNATC 1 cut(s) 569
BsaHI GRCGYC 2 cut(s) 78, 114
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 2 cut(s) 187, 555
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 223
Bse8I GATNNNNATC 1 cut(s) 569
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 187, 555
BseJI GATNNNNATC 1 cut(s) 569
BseLI CCNNNNNNNGG 2 cut(s) 163, 223
BseMII CTCAG 1 cut(s) 326
BseXI GCAGC 2 cut(s) 112, 115
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 668
BshNI GGYRCC 1 cut(s) 632
BsiEI CGRYCG 1 cut(s) 174
BsiWI CGTACG 1 cut(s) 343
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 163, 223
BsmAI GTCTC 4 cut(s) 172, 238, 543, 713
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 1 cut(s) 668
Bso31I GGTCTC 1 cut(s) 172
Bsp143I GATC 4 cut(s) 54, 331, 543, 570
BspACI CCGC 3 cut(s) 136, 157, 407
BspANI GGCC 1 cut(s) 668
BspCNI CTCAG 1 cut(s) 327
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 211, 634
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 187, 555
BssMI GATC 4 cut(s) 54, 331, 543, 570
BssNAI GTATAC 1 cut(s) 729
BssNI GRCGYC 2 cut(s) 78, 114
BssSI CACGAG 1 cut(s) 472
BssT1I CCWWGG 2 cut(s) 187, 555
Bst1107I GTATAC 1 cut(s) 729
Bst2BI CACGAG 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 311
Bst4CI ACNGT 6 cut(s) 49, 258, 348, 500, 527, 532
BstACI GRCGYC 2 cut(s) 78, 114
BstBAI YACGTR 1 cut(s) 732
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 335
BstFNI CGCG 1 cut(s) 377
BstHHI GCGC 2 cut(s) 186, 655
BstKTI GATC 4 cut(s) 57, 334, 546, 573
BstMAI GTCTC 4 cut(s) 172, 238, 543, 713
BstMBI GATC 4 cut(s) 54, 331, 543, 570
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 233
BstSNI TACGTA 1 cut(s) 732
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 112, 115
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 643
BstYI RGATCY 1 cut(s) 54
BstZ17I GTATAC 1 cut(s) 729
BsuRI GGCC 1 cut(s) 668
BtgZI GCGATG 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 240
CciI TCATGA 1 cut(s) 318
CfoI GCGC 2 cut(s) 186, 655
Cfr13I GGNCC 1 cut(s) 616
CseI GACGC 2 cut(s) 328, 340
Csp6I GTAC 5 cut(s) 292, 344, 528, 633, 733
CviAII CATG 5 cut(s) 160, 319, 509, 521, 656
CviJI RGCY 7 cut(s) 153, 212, 238, 314, 560, 578, 668
CviKI_1 RGCY 7 cut(s) 153, 212, 238, 314, 560, 578, 668
CviQI GTAC 5 cut(s) 292, 344, 528, 633, 733
DdeI CTNAG 1 cut(s) 335
DpnI GATC 4 cut(s) 56, 333, 545, 572
DpnII GATC 4 cut(s) 54, 331, 543, 570
Eco105I TACGTA 1 cut(s) 732
Eco130I CCWWGG 2 cut(s) 187, 555
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 616
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 2 cut(s) 187, 555
ErhI CCWWGG 2 cut(s) 187, 555
FaeI CATG 5 cut(s) 163, 322, 512, 524, 659
FaiI YATR 9 cut(s) 161, 320, 401, 510, 522, 630, 657, 702, 729
FaqI GGGAC 1 cut(s) 58
FatI CATG 5 cut(s) 159, 318, 508, 520, 655
FauI CCCGC 2 cut(s) 143, 164
FbaI TGATCA 1 cut(s) 543
FblI GTMKAC 1 cut(s) 728
Fnu4HI GCNGC 2 cut(s) 126, 129
Fsp4HI GCNGC 2 cut(s) 126, 129
FspBI CTAG 1 cut(s) 108
GlaI GCGC 2 cut(s) 185, 654
GluI GCNGC 2 cut(s) 126, 129
HaeIII GGCC 1 cut(s) 668
HgaI GACGC 2 cut(s) 328, 340
HhaI GCGC 2 cut(s) 186, 655
Hin1I GRCGYC 2 cut(s) 78, 114
Hin1II CATG 5 cut(s) 163, 322, 512, 524, 659
Hin6I GCGC 2 cut(s) 184, 653
HinP1I GCGC 2 cut(s) 184, 653
HinfI GANTC 4 cut(s) 34, 246, 364, 748
Hpy166II GTNNAC 1 cut(s) 729
Hpy188I TCNGA 4 cut(s) 209, 336, 548, 595
Hpy188III TCNNGA 5 cut(s) 52, 175, 298, 319, 682
Hpy8I GTNNAC 1 cut(s) 729
Hpy99I CGWCG 6 cut(s) 77, 80, 224, 344, 356, 396
HpyAV CCTTC 4 cut(s) 157, 373, 418, 658
HpyCH4III ACNGT 6 cut(s) 49, 258, 348, 500, 527, 532
HpyCH4IV ACGT 4 cut(s) 78, 114, 327, 731
HpyCH4V TGCA 1 cut(s) 520
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 4 cut(s) 78, 114, 327, 731
Hsp92I GRCGYC 2 cut(s) 78, 114
Hsp92II CATG 5 cut(s) 163, 322, 512, 524, 659
HspAI GCGC 2 cut(s) 184, 653
KpnI GGTACC 1 cut(s) 636
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 4 cut(s) 54, 331, 543, 570
LmnI GCTCC 2 cut(s) 158, 209
LpnPI CCDG 5 cut(s) 67, 252, 296, 323, 353
Lsp1109I GCAGC 2 cut(s) 112, 115
MaeI CTAG 1 cut(s) 108
MaeII ACGT 4 cut(s) 78, 114, 327, 731
MaeIII GTNAC 2 cut(s) 10, 110
MalI GATC 4 cut(s) 56, 333, 545, 572
MboI GATC 4 cut(s) 54, 331, 543, 570
MboII GAAGA 1 cut(s) 77
MflI RGATCY 1 cut(s) 54
MlyI GAGTC 2 cut(s) 240, 373
MmeI TCCRAC 2 cut(s) 187, 303
MnlI CCTC 9 cut(s) 35, 68, 80, 107, 227, 403, 468, 505, 731
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 2 cut(s) 468, 582
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 4 cut(s) 54, 331, 543, 570
NlaIII CATG 5 cut(s) 163, 322, 512, 524, 659
NlaIV GGNNCC 2 cut(s) 211, 634
NmuCI GTSAC 2 cut(s) 10, 110
PagI TCATGA 1 cut(s) 318
PcsI WCGNNNNNNNCGW 2 cut(s) 348, 381
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 1 cut(s) 34
Pfl23II CGTACG 1 cut(s) 343
PflFI GACNNNGTC 2 cut(s) 77, 218
PkrI GCNGC 2 cut(s) 127, 130
PleI GAGTC 2 cut(s) 240, 372
PpsI GAGTC 2 cut(s) 240, 372
Ppu21I YACGTR 1 cut(s) 732
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspLI CGTACG 1 cut(s) 343
PspN4I GGNNCC 2 cut(s) 211, 634
PspPI GGNCC 1 cut(s) 616
PsuI RGATCY 1 cut(s) 54
PsyI GACNNNGTC 2 cut(s) 77, 218
RsaI GTAC 5 cut(s) 293, 345, 529, 634, 734
RsaNI GTAC 5 cut(s) 292, 344, 528, 633, 733
SaqAI TTAA 2 cut(s) 468, 582
SatI GCNGC 2 cut(s) 126, 129
Sau3AI GATC 4 cut(s) 54, 331, 543, 570
Sau96I GGNCC 1 cut(s) 616
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 2 cut(s) 240, 373
ScrFI CCNGG 1 cut(s) 311
SfcI CTRYAG 1 cut(s) 233
SinI GGWCC 1 cut(s) 616
SnaBI TACGTA 1 cut(s) 732
SsiI CCGC 3 cut(s) 136, 157, 407
SspMI CTAG 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 309
StyI CCWWGG 2 cut(s) 187, 555
TaaI ACNGT 6 cut(s) 49, 258, 348, 500, 527, 532
TaiI ACGT 4 cut(s) 81, 117, 330, 734
TaqI TCGA 3 cut(s) 72, 174, 297
TaqII GACCGA 2 cut(s) 160, 633
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 2 cut(s) 468, 582
Tru9I TTAA 2 cut(s) 468, 582
TseFI GTSAC 2 cut(s) 10, 110
TseI GCWGC 2 cut(s) 125, 128
Tsp45I GTSAC 2 cut(s) 10, 110
TspDTI ATGAA 5 cut(s) 17, 176, 307, 335, 551
TspGWI ACGGA 2 cut(s) 380, 623
Tth111I GACNNNGTC 2 cut(s) 77, 218
VpaK11BI GGWCC 1 cut(s) 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 728
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 1 cut(s) 108
ZraI GACGTC 2 cut(s) 79, 115
ZrmI AGTACT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.