Rh3DG182800
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001369
Physical Location & Seq
Reverse (-)
16230570 .. 16231010
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG182800.1

Sequence Viewer

Length: 441 bp
ATGATTGCGGAGGCAAATAGGTTGAAGGTCAAGTCAGTAGAGAACAAGCAACGCATTGTCGTTTTTAACACGAGGAGCAAAGGGAAAGACGGGAACAGTAGTTTGCATGAGGTTTTGCACGACAATACCGTGATGAACTTGATCAGAGACACCAAGGCTGTGATGGAGATCAAGGCTTTTAAGGAGTTTTCGGATTTGCTAACAACGGATTTGGACCGAGTGTGTTATGGTACCAAGAATGTGGAGATGGCGCATGAGTTAAAGGCCATTGAAACGCTCGTGATTACCGACTTGTACGAGTGTGTAGAGATTGAGACGAGGTTTAAGTACATAGGGTTTGTGGAGTCGGTGAAAAAGGCAGGAGGGAAGGCGTTTGTGTTTTCGTCAGGGCATGTATCTGAAGTGGCGCTAGCTAATCTGACAGCATTGTGGCTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

146

Amino Acids

16.46

Weight (kDa)

6.91

Isoelectric Point (pI)

24.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eRF1_3 PF03465 54 - 138 2.2e-12 eRF1 domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 230
AciI CCGC 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 3 cut(s) 232, 296, 329
AgsI TTSAA 2 cut(s) 25, 272
AluBI AGCT 1 cut(s) 413
AluI AGCT 1 cut(s) 413
Alw26I GTCTC 2 cut(s) 141, 308
AoxI GGCC 1 cut(s) 264
Asp718I GGTACC 1 cut(s) 230
AspLEI GCGC 2 cut(s) 253, 409
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 361
AsuNHI GCTAGC 1 cut(s) 409
AvaII GGWCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 230
BauI CACGAG 2 cut(s) 70, 278
BccI CCATC 2 cut(s) 157, 241
BclI TGATCA 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 141, 308
BfaI CTAG 1 cut(s) 410
BfoI RGCGCY 1 cut(s) 410
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 232
BmtI GCTAGC 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 153
Bse8I GATNNNNATC 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 153
BseJI GATNNNNATC 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 88
BshFI GGCC 1 cut(s) 266
BshNI GGYRCC 1 cut(s) 230
BsmAI GTCTC 2 cut(s) 141, 308
BsmBI CGTCTC 1 cut(s) 308
BsnI GGCC 1 cut(s) 266
Bsp143I GATC 2 cut(s) 141, 168
BspACI CCGC 1 cut(s) 8
BspANI GGCC 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 232
BspOI GCTAGC 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 141, 168
BssSI CACGAG 2 cut(s) 70, 278
BssT1I CCWWGG 1 cut(s) 153
Bst2BI CACGAG 2 cut(s) 70, 278
Bst4CI ACNGT 2 cut(s) 98, 130
BstC8I GCNNGC 1 cut(s) 411
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 2 cut(s) 253, 409
BstKTI GATC 2 cut(s) 144, 171
BstMAI GTCTC 2 cut(s) 141, 308
BstMBI GATC 2 cut(s) 141, 168
BstNSI RCATGY 1 cut(s) 395
BstXI CCANNNNNNTGG 1 cut(s) 241
BsuRI GGCC 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 411
CfoI GCGC 2 cut(s) 253, 409
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 3 cut(s) 231, 295, 328
CviAII CATG 3 cut(s) 107, 254, 392
CviJI RGCY 5 cut(s) 158, 176, 266, 413, 433
CviKI_1 RGCY 5 cut(s) 158, 176, 266, 413, 433
CviQI GTAC 3 cut(s) 231, 295, 328
DpnI GATC 2 cut(s) 143, 170
DpnII GATC 2 cut(s) 141, 168
Eco130I CCWWGG 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 420
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 3 cut(s) 110, 257, 395
FaiI YATR 5 cut(s) 108, 228, 255, 332, 393
FatI CATG 3 cut(s) 106, 253, 391
FbaI TGATCA 1 cut(s) 141
FspBI CTAG 1 cut(s) 410
GlaI GCGC 2 cut(s) 252, 408
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 1 cut(s) 266
HhaI GCGC 2 cut(s) 253, 409
Hin1II CATG 3 cut(s) 110, 257, 395
Hin6I GCGC 2 cut(s) 251, 407
HinP1I GCGC 2 cut(s) 251, 407
HinfI GANTC 1 cut(s) 344
HphI GGTGA 1 cut(s) 361
Hpy188I TCNGA 5 cut(s) 146, 193, 400, 420, 440
Hpy188III TCNNGA 1 cut(s) 280
HpyAV CCTTC 2 cut(s) 19, 361
HpyCH4III ACNGT 2 cut(s) 98, 130
HpyCH4V TGCA 2 cut(s) 106, 118
Hsp92II CATG 3 cut(s) 110, 257, 395
HspAI GCGC 2 cut(s) 251, 407
KpnI GGTACC 1 cut(s) 234
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 141, 168
LmnI GCTCC 1 cut(s) 75
LpnPI CCDG 2 cut(s) 345, 372
MaeI CTAG 1 cut(s) 410
MalI GATC 2 cut(s) 143, 170
MboI GATC 2 cut(s) 141, 168
MlyI GAGTC 1 cut(s) 353
MnlI CCTC 5 cut(s) 4, 66, 103, 312, 356
MseI TTAA 4 cut(s) 66, 180, 260, 324
NdeII GATC 2 cut(s) 141, 168
NheI GCTAGC 1 cut(s) 409
NlaIII CATG 3 cut(s) 110, 257, 395
NlaIV GGNNCC 1 cut(s) 232
NspI RCATGY 1 cut(s) 395
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 285
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 214
RsaI GTAC 3 cut(s) 232, 296, 329
RsaNI GTAC 3 cut(s) 231, 295, 328
SaqAI TTAA 4 cut(s) 66, 180, 260, 324
Sau3AI GATC 2 cut(s) 141, 168
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 1 cut(s) 353
SetI ASST 5 cut(s) 23, 30, 114, 323, 415
SinI GGWCC 1 cut(s) 214
SsiI CCGC 1 cut(s) 8
SspMI CTAG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 98, 130
TaqII GACCGA 1 cut(s) 231
TatI WGTACW 1 cut(s) 327
Tru1I TTAA 4 cut(s) 66, 180, 260, 324
Tru9I TTAA 4 cut(s) 66, 180, 260, 324
TspDTI ATGAA 1 cut(s) 149
TspGWI ACGGA 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 214
XceI RCATGY 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.