pycom14g09460
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001369
Physical Location & Seq
Reverse (-)
11407088 .. 11407525
438 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g09460.2

Sequence Viewer

Length: 267 bp
ATGGAAAAACCACCTGAGTGGTACCAAATCATGAACTTAGAAGATGAAATCCCGGTCGTCTACAACCTTATCGCCCATGGCGACGTCATCACCACAGCCACGACCCGAAAAAGTAAACACAGGTCGCCCAAGAACAAGAGCTTCCGGTTAACCCCCGGAATCTCGATCGCCGCCATTGATTACCAGAAAGACTCCTCTATGGTGCGGGTCCAGGGCAAACGCCACCGTCAACTAGTACGTCCAGACGGGGTTGTTTCATACCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

89

Amino Acids

10.11

Weight (kDa)

9.75

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
Acc65I GGTACC 1 cut(s) 21
AccB1I GGYRCC 1 cut(s) 21
AccI GTMKAC 1 cut(s) 60
AciI CCGC 2 cut(s) 171, 205
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 2 cut(s) 23, 237
AhlI ACTAGT 1 cut(s) 232
AjnI CCWGG 1 cut(s) 210
AleI CACNNNNGTG 1 cut(s) 16
AluBI AGCT 1 cut(s) 141
AluI AGCT 1 cut(s) 141
Asp718I GGTACC 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 2 cut(s) 53, 156
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 21
BciT130I CCWGG 1 cut(s) 212
BcnI CCSGG 2 cut(s) 53, 156
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 263
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 3 cut(s) 53, 156, 212
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 23, 209
BmrFI CCNGG 3 cut(s) 53, 156, 212
BpuMI CCSGG 2 cut(s) 53, 156
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 76, 154, 211
BsaWI WCCGGW 1 cut(s) 144
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 3 cut(s) 76, 154, 211
BseMII CTCAG 1 cut(s) 6
BseRI GAGGAG 1 cut(s) 184
Bsh1285I CGRYCG 2 cut(s) 57, 168
BshNI GGYRCC 1 cut(s) 21
BsiEI CGRYCG 2 cut(s) 57, 168
BsiSI CCGG 3 cut(s) 53, 145, 156
Bsp143I GATC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 2 cut(s) 171, 205
BspCNI CTCAG 1 cut(s) 7
BspHI TCATGA 1 cut(s) 30
BspLI GGNNCC 2 cut(s) 23, 209
BspT107I GGYRCC 1 cut(s) 21
BssECI CCNNGG 3 cut(s) 76, 154, 211
BssMI GATC 1 cut(s) 165
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 76
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 15, 37
BstDSI CCRYGG 1 cut(s) 76
BstKTI GATC 1 cut(s) 168
BstMBI GATC 1 cut(s) 165
BstMCI CGRYCG 2 cut(s) 57, 168
BstNI CCWGG 1 cut(s) 212
BstSCI CCNGG 3 cut(s) 51, 154, 210
BstSFI CTRYAG 1 cut(s) 263
BstXI CCANNNNNNTGG 1 cut(s) 18
BtgI CCRYGG 1 cut(s) 76
CciI TCATGA 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 22, 236
CviAII CATG 2 cut(s) 31, 77
CviJI RGCY 2 cut(s) 98, 141
CviKI_1 RGCY 2 cut(s) 98, 141
CviQI GTAC 2 cut(s) 22, 236
DdeI CTNAG 2 cut(s) 15, 37
DpnI GATC 1 cut(s) 167
DpnII GATC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 2 cut(s) 34, 80
FaiI YATR 4 cut(s) 32, 78, 200, 259
FatI CATG 2 cut(s) 30, 76
FauI CCCGC 1 cut(s) 198
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 1 cut(s) 233
GluI GCNGC 1 cut(s) 171
HapII CCGG 3 cut(s) 53, 145, 156
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 2 cut(s) 34, 80
HincII GTYRAC 2 cut(s) 150, 230
HindII GTYRAC 2 cut(s) 150, 230
HinfI GANTC 2 cut(s) 159, 191
HpaI GTTAAC 1 cut(s) 150
HpaII CCGG 3 cut(s) 53, 145, 156
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 4 cut(s) 61, 116, 150, 230
Hpy188III TCNNGA 3 cut(s) 31, 163, 242
Hpy8I GTNNAC 4 cut(s) 61, 116, 150, 230
Hpy99I CGWCG 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 2 cut(s) 84, 238
HpyF3I CTNAG 2 cut(s) 15, 37
HpySE526I ACGT 2 cut(s) 84, 238
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 2 cut(s) 34, 80
KpnI GGTACC 1 cut(s) 25
KspAI GTTAAC 1 cut(s) 150
Kzo9I GATC 1 cut(s) 165
LpnPI CCDG 9 cut(s) 27, 66, 106, 158, 169, 197, 197, 224, 255
MaeI CTAG 1 cut(s) 233
MaeII ACGT 2 cut(s) 84, 238
MalI GATC 1 cut(s) 167
MboI GATC 1 cut(s) 165
MboII GAAGA 1 cut(s) 53
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 1 cut(s) 205
MseI TTAA 1 cut(s) 149
MslI CAYNNNNRTG 1 cut(s) 16
MspI CCGG 3 cut(s) 53, 145, 156
MspR9I CCNGG 3 cut(s) 53, 156, 212
MvaI CCWGG 1 cut(s) 212
NciI CCSGG 2 cut(s) 53, 156
NcoI CCATGG 1 cut(s) 76
NdeII GATC 1 cut(s) 165
NlaIII CATG 2 cut(s) 34, 80
NlaIV GGNNCC 2 cut(s) 23, 209
OliI CACNNNNGTG 1 cut(s) 16
PagI TCATGA 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 1 cut(s) 172
Ple19I CGATCG 1 cut(s) 168
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 23, 209
PspPI GGNCC 1 cut(s) 208
PvuI CGATCG 1 cut(s) 168
RsaI GTAC 2 cut(s) 23, 237
RsaNI GTAC 2 cut(s) 22, 236
RseI CAYNNNNRTG 1 cut(s) 16
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 165
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 53, 156, 212
SetI ASST 7 cut(s) 16, 69, 87, 125, 143, 241, 264
SfcI CTRYAG 1 cut(s) 263
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 16
SpeI ACTAGT 1 cut(s) 232
SsiI CCGC 2 cut(s) 171, 205
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 3 cut(s) 51, 154, 210
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 2 cut(s) 87, 241
TaqI TCGA 1 cut(s) 164
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TspDTI ATGAA 3 cut(s) 47, 60, 246
VpaK11BI GGWCC 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 1 cut(s) 233
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.