Rroxscaffold_6G00410020
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001369
Physical Location & Seq
Reverse (-)
32494988 .. 32495533
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410020.1

Sequence Viewer

Length: 507 bp
ATGACGTCTGGTTCGTTTTCAACCTCATCTCCCCCGGTGACGTCATCATCGCCACCACCACCCGCAACGTCAAATACAGCTCCAGCGTCAAGGTCATTCTCGAAATCTCGATCACCTCCGCCGTGGACTACCACAAGGACTCGTCCACCCTGCGCGTGCAGGGCACGACCCTCACCACCAACTAGCACGTCAAGAAGGGACAAGGTCTCGGTCGAGAAACTGCGCCAAGAGTTCAAAAAGGTCGTCGGAGCCGACCTCGCCATGGTGGGGCCCAGGCAGACCAAGCTCTGCGCCAAGGTGGAAACTCACTCCAACCCTAACAAGTACTTCGAGAACATTTTCCGGGCTTTCGTGAAGCACGTTGATCTGAGCGTCGTACGGTGCTTTGTAATAGGGAGTCAGGGAAACGCGAAGGACGAGTTCCGTCGTTTTATGATTGCGGAGGCGAATAGGTTAATTGAAGGTCAAGTCAGTAGAGAACAACAAGCAACGCATTGTCATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

168

Amino Acids

18.58

Weight (kDa)

10.39

Isoelectric Point (pI)

64.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 8, 44
AccII CGCG 2 cut(s) 155, 410
AciI CCGC 3 cut(s) 63, 119, 440
AcyI GRCGYC 2 cut(s) 5, 41
AfaI GTAC 2 cut(s) 326, 378
AfiI CCNNNNNNNGG 2 cut(s) 262, 267
AgsI TTSAA 3 cut(s) 21, 235, 461
AjiI CACGTC 1 cut(s) 189
AjnI CCWGG 1 cut(s) 272
AluBI AGCT 2 cut(s) 80, 286
AluI AGCT 2 cut(s) 80, 286
Alw26I GTCTC 1 cut(s) 211
AoxI GGCC 1 cut(s) 269
ApaI GGGCCC 1 cut(s) 273
ArsI GACNNNNNNTTYG 3 cut(s) 27, 228, 260
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 3 cut(s) 155, 225, 293
AspS9I GGNCC 2 cut(s) 269, 270
AsuC2I CCSGG 2 cut(s) 35, 344
AsuHPI GGTGA 3 cut(s) 49, 105, 165
BaeGI GKGCMC 2 cut(s) 166, 273
BanII GRGCYC 1 cut(s) 273
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 274
BcnI CCSGG 2 cut(s) 35, 344
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 183
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 3 cut(s) 35, 274, 344
BmgBI CACGTC 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 269, 270
BmiI GGNNCC 3 cut(s) 250, 270, 271
BmrFI CCNGG 3 cut(s) 35, 274, 344
BplI GAGNNNNNCTC 2 cut(s) 240, 272
BpmI CTGGAG 1 cut(s) 66
BpuMI CCSGG 2 cut(s) 35, 344
BsaHI GRCGYC 2 cut(s) 5, 41
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 5 cut(s) 33, 122, 261, 272, 294
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 267
BseBI CCWGG 1 cut(s) 274
BseDI CCNNGG 5 cut(s) 33, 122, 261, 272, 294
BseLI CCNNNNNNNGG 2 cut(s) 262, 267
BseMII CTCAG 1 cut(s) 359
BseSI GKGCMC 2 cut(s) 166, 273
BsgI GTGCAG 1 cut(s) 178
Bsh1236I CGCG 2 cut(s) 155, 410
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 1 cut(s) 271
BsiEI CGRYCG 1 cut(s) 213
BsiSI CCGG 2 cut(s) 35, 343
BsiWI CGTACG 1 cut(s) 376
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 262, 267
BsmAI GTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 1 cut(s) 271
Bso31I GGTCTC 1 cut(s) 211
Bsp120I GGGCCC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 166, 273
Bsp143I GATC 2 cut(s) 110, 364
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 3 cut(s) 63, 119, 440
BspANI GGCC 1 cut(s) 271
BspCNI CTCAG 1 cut(s) 360
BspFNI CGCG 2 cut(s) 155, 410
BspLI GGNNCC 3 cut(s) 250, 270, 271
BspTNI GGTCTC 1 cut(s) 211
BssECI CCNNGG 5 cut(s) 33, 122, 261, 272, 294
BssMI GATC 2 cut(s) 110, 364
BssNI GRCGYC 2 cut(s) 5, 41
BssT1I CCWWGG 2 cut(s) 261, 294
Bst2UI CCWGG 1 cut(s) 274
Bst4CI ACNGT 1 cut(s) 381
BstACI GRCGYC 2 cut(s) 5, 41
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 1 cut(s) 368
BstDSI CCRYGG 2 cut(s) 122, 261
BstFNI CGCG 2 cut(s) 155, 410
BstHHI GCGC 3 cut(s) 155, 225, 293
BstKTI GATC 2 cut(s) 113, 367
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 2 cut(s) 110, 364
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 161, 257, 283
BstNI CCWGG 1 cut(s) 274
BstSCI CCNGG 3 cut(s) 33, 272, 342
BstSLI GKGCMC 2 cut(s) 166, 273
BstUI CGCG 2 cut(s) 155, 410
BsuRI GGCC 1 cut(s) 271
BtgI CCRYGG 2 cut(s) 122, 261
BtgZI GCGATG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 157
CfoI GCGC 3 cut(s) 155, 225, 293
Cfr13I GGNCC 2 cut(s) 269, 270
CseI GACGC 2 cut(s) 75, 361
Csp6I GTAC 2 cut(s) 325, 377
CviAII CATG 1 cut(s) 262
CviJI RGCY 5 cut(s) 80, 251, 271, 286, 347
CviKI_1 RGCY 5 cut(s) 80, 251, 271, 286, 347
CviQI GTAC 2 cut(s) 325, 377
DdeI CTNAG 1 cut(s) 368
DpnI GATC 2 cut(s) 112, 366
DpnII GATC 2 cut(s) 110, 364
EciI GGCGGA 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 261, 294
Eco24I GRGCYC 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 272
EcoT14I CCWWGG 2 cut(s) 261, 294
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 261, 294
FaeI CATG 1 cut(s) 265
FaiI YATR 2 cut(s) 263, 434
FaqI GGGAC 1 cut(s) 212
FatI CATG 1 cut(s) 261
FauI CCCGC 1 cut(s) 70
FriOI GRGCYC 1 cut(s) 273
FspBI CTAG 1 cut(s) 183
GlaI GCGC 3 cut(s) 154, 224, 292
GsuI CTGGAG 1 cut(s) 66
HaeIII GGCC 1 cut(s) 271
HapII CCGG 2 cut(s) 35, 343
HgaI GACGC 2 cut(s) 75, 361
HhaI GCGC 3 cut(s) 155, 225, 293
Hin1I GRCGYC 2 cut(s) 5, 41
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 3 cut(s) 153, 223, 291
HinP1I GCGC 3 cut(s) 153, 223, 291
HinfI GANTC 2 cut(s) 139, 397
HpaII CCGG 2 cut(s) 35, 343
HphI GGTGA 3 cut(s) 49, 105, 165
Hpy166II GTNNAC 2 cut(s) 126, 146
Hpy188I TCNGA 2 cut(s) 248, 369
Hpy188III TCNNGA 6 cut(s) 100, 108, 192, 214, 331, 352
Hpy8I GTNNAC 2 cut(s) 126, 146
Hpy99I CGWCG 3 cut(s) 248, 377, 429
HpyAV CCTTC 3 cut(s) 189, 406, 455
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4IV ACGT 5 cut(s) 5, 41, 68, 188, 360
HpyCH4V TGCA 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 257, 283
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 5 cut(s) 5, 41, 68, 188, 360
Hsp92I GRCGYC 2 cut(s) 5, 41
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 3 cut(s) 153, 223, 291
Kzo9I GATC 2 cut(s) 110, 364
LmnI GCTCC 2 cut(s) 85, 248
LpnPI CCDG 8 cut(s) 48, 96, 145, 163, 259, 286, 356, 386
MaeI CTAG 1 cut(s) 183
MaeII ACGT 5 cut(s) 5, 41, 68, 188, 360
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 112, 366
MboI GATC 2 cut(s) 110, 364
MhlI GDGCHC 2 cut(s) 166, 273
MluCI AATT 1 cut(s) 456
MlyI GAGTC 2 cut(s) 133, 406
MmeI TCCRAC 2 cut(s) 226, 336
MnlI CCTC 5 cut(s) 34, 126, 181, 266, 436
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 2 cut(s) 455, 505
MspI CCGG 2 cut(s) 35, 343
MspR9I CCNGG 3 cut(s) 35, 274, 344
MvaI CCWGG 1 cut(s) 274
MvnI CGCG 2 cut(s) 155, 410
MwoI GCNNNNNNNGC 3 cut(s) 161, 257, 283
NciI CCSGG 2 cut(s) 35, 344
NcoI CCATGG 1 cut(s) 261
NdeII GATC 2 cut(s) 110, 364
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 3 cut(s) 250, 270, 271
NmuCI GTSAC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 4 cut(s) 11, 249, 357, 414
PdmI GAANNNNTTC 1 cut(s) 338
Pfl23II CGTACG 1 cut(s) 376
PflFI GACNNNGTC 1 cut(s) 203
PleI GAGTC 2 cut(s) 133, 405
PpsI GAGTC 2 cut(s) 133, 405
Psp6I CCWGG 1 cut(s) 272
PspGI CCWGG 1 cut(s) 272
PspLI CGTACG 1 cut(s) 376
PspN4I GGNNCC 3 cut(s) 250, 270, 271
PspOMI GGGCCC 1 cut(s) 269
PspPI GGNCC 2 cut(s) 269, 270
PsyI GACNNNGTC 1 cut(s) 203
RsaI GTAC 2 cut(s) 326, 378
RsaNI GTAC 2 cut(s) 325, 377
SaqAI TTAA 2 cut(s) 455, 505
Sau3AI GATC 2 cut(s) 110, 364
Sau96I GGNCC 2 cut(s) 269, 270
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 2 cut(s) 133, 406
ScrFI CCNGG 3 cut(s) 35, 274, 344
SduI GDGCHC 2 cut(s) 166, 273
Sse9I AATT 1 cut(s) 456
SsiI CCGC 3 cut(s) 63, 119, 440
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 3 cut(s) 33, 272, 342
StyI CCWWGG 2 cut(s) 261, 294
TaaI ACNGT 1 cut(s) 381
TaiI ACGT 5 cut(s) 8, 44, 71, 191, 363
TaqI TCGA 4 cut(s) 101, 109, 213, 330
TaqII GACCGA 1 cut(s) 199
TasI AATT 1 cut(s) 456
TatI WGTACW 1 cut(s) 324
Tru1I TTAA 2 cut(s) 455, 505
Tru9I TTAA 2 cut(s) 455, 505
TseFI GTSAC 1 cut(s) 37
Tsp45I GTSAC 1 cut(s) 37
TspGWI ACGGA 1 cut(s) 413
Tth111I GACNNNGTC 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 1 cut(s) 183
ZraI GACGTC 2 cut(s) 6, 42
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.