Rh3DG182900
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001369
Physical Location & Seq
Reverse (-)
16231273 .. 16231527
255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG182900.1

Sequence Viewer

Length: 255 bp
ATGAAACTCGTCATAGAAAACAATATCGTTGTGAATCAACGAGGGACGGTCAAGATCCTCCCCGAAGGCCTCGACGACGTCTGGTTTGTTTTCAACCTCATCTCCCCCGGTGACGTCATCATCGCCGCCACCACCCGCAGCGTCAATTACAGCTCCCGCATGAAGGTCATTCTCGAAATCTCGATCACCGCCGTGGACTACCACAAGGACTCGTCCACCCTGAGCGTGCAGGGCACGACATTCACCACCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

84

Amino Acids

9.29

Weight (kDa)

5.61

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pelota_N PF26356 1 - 83 1.4e-18 Pelota N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 81, 117
AciI CCGC 4 cut(s) 126, 136, 157, 189
AclWI GGATC 1 cut(s) 49
AcyI GRCGYC 2 cut(s) 78, 114
AfiI CCNNNNNNNGG 1 cut(s) 163
AgsI TTSAA 1 cut(s) 94
AleI CACNNNNGTG 1 cut(s) 191
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
AlwI GGATC 1 cut(s) 49
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 138
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 3 cut(s) 122, 178, 235
BaeGI GKGCMC 1 cut(s) 236
BbvI GCAGC 1 cut(s) 150
BceAI ACGGC 1 cut(s) 176
BcnI CCSGG 1 cut(s) 108
BfaI CTAG 1 cut(s) 253
BisI GCNGC 2 cut(s) 126, 139
BlsI GCNGC 2 cut(s) 127, 140
Bme1390I CCNGG 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 78, 114
BsaJI CCNNGG 2 cut(s) 106, 192
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 106, 192
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 1 cut(s) 150
BsgI GTGCAG 1 cut(s) 248
BshFI GGCC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 2 cut(s) 54, 183
BspACI CCGC 4 cut(s) 126, 136, 157, 189
BspANI GGCC 1 cut(s) 69
BspCNI CTCAG 1 cut(s) 213
BspPI GGATC 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 106, 192
BssMI GATC 2 cut(s) 54, 183
BssNI GRCGYC 2 cut(s) 78, 114
Bst4CI ACNGT 1 cut(s) 49
BstACI GRCGYC 2 cut(s) 78, 114
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 1 cut(s) 221
BstDSI CCRYGG 1 cut(s) 192
BstKTI GATC 2 cut(s) 57, 186
BstMBI GATC 2 cut(s) 54, 183
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 54
BstYI RGATCY 1 cut(s) 54
BsuRI GGCC 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 192
BtgZI GCGATG 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 227
CseI GACGC 1 cut(s) 130
CviAII CATG 1 cut(s) 160
CviJI RGCY 2 cut(s) 69, 153
CviKI_1 RGCY 2 cut(s) 69, 153
DdeI CTNAG 1 cut(s) 221
DpnI GATC 2 cut(s) 56, 185
DpnII GATC 2 cut(s) 54, 183
Eco147I AGGCCT 1 cut(s) 69
FaeI CATG 1 cut(s) 163
FaiI YATR 2 cut(s) 14, 161
FaqI GGGAC 1 cut(s) 58
FatI CATG 1 cut(s) 159
FauI CCCGC 2 cut(s) 143, 164
Fnu4HI GCNGC 2 cut(s) 126, 139
Fsp4HI GCNGC 2 cut(s) 126, 139
FspBI CTAG 1 cut(s) 253
GluI GCNGC 2 cut(s) 126, 139
HaeIII GGCC 1 cut(s) 69
HapII CCGG 1 cut(s) 108
HgaI GACGC 1 cut(s) 130
Hin1I GRCGYC 2 cut(s) 78, 114
Hin1II CATG 1 cut(s) 163
HinfI GANTC 2 cut(s) 34, 209
HpaII CCGG 1 cut(s) 108
HphI GGTGA 3 cut(s) 122, 178, 235
Hpy166II GTNNAC 2 cut(s) 196, 216
Hpy188III TCNNGA 3 cut(s) 52, 173, 181
Hpy8I GTNNAC 2 cut(s) 196, 216
Hpy99I CGWCG 2 cut(s) 77, 80
HpyAV CCTTC 2 cut(s) 59, 157
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 2 cut(s) 78, 114
HpyCH4V TGCA 1 cut(s) 229
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 2 cut(s) 78, 114
Hsp92I GRCGYC 2 cut(s) 78, 114
Hsp92II CATG 1 cut(s) 163
Kzo9I GATC 2 cut(s) 54, 183
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 4 cut(s) 67, 121, 215, 233
Lsp1109I GCAGC 1 cut(s) 150
MaeI CTAG 1 cut(s) 253
MaeII ACGT 2 cut(s) 78, 114
MaeIII GTNAC 1 cut(s) 110
MalI GATC 2 cut(s) 56, 185
MboI GATC 2 cut(s) 54, 183
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 1 cut(s) 145
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 4 cut(s) 35, 68, 80, 107
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 231
NciI CCSGG 1 cut(s) 108
NdeII GATC 2 cut(s) 54, 183
NlaIII CATG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 110
OliI CACNNNNGTG 1 cut(s) 191
PceI AGGCCT 1 cut(s) 69
PfeI GAWTC 1 cut(s) 34
PflFI GACNNNGTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 127, 140
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PsuI RGATCY 1 cut(s) 54
PsyI GACNNNGTC 1 cut(s) 77
RseI CAYNNNNRTG 1 cut(s) 191
SatI GCNGC 2 cut(s) 126, 139
Sau3AI GATC 2 cut(s) 54, 183
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 236
SetI ASST 5 cut(s) 81, 99, 117, 155, 168
SmiMI CAYNNNNRTG 1 cut(s) 191
Sse9I AATT 1 cut(s) 145
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 4 cut(s) 126, 136, 157, 189
SspMI CTAG 1 cut(s) 253
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 2 cut(s) 81, 117
TaqI TCGA 3 cut(s) 72, 174, 182
TasI AATT 1 cut(s) 145
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 34
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 138
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 17, 176
Tth111I GACNNNGTC 1 cut(s) 77
XspI CTAG 1 cut(s) 253
ZraI GACGTC 2 cut(s) 79, 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.