Prupe.7G122700_v2.0.a1
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001369
Physical Location & Seq
Reverse (-)
14546739 .. 14547251
513 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G122700.1

Sequence Viewer

Length: 513 bp
ATGAAGGAAGACTTTCGTCGTTTTTTGATTGGGGAGGAGCAGAGGTTGAAGTTGAAGTCTATTGAGAACAACAAATCAAGGATTGTCGTTGTTAATACGAGCAAAGGCGACAATAATAATTGTAGTAGCAGCTTGCATGAGGTTTTGCATGACAAAGCGGTCATGAATTTGATCAAGGACACCAAGGCTGCGATGGAGATCAGGGCTTACAAGGAGTTTTCGGACATGGTATCGAGCAATTCGGATCAAGCGTGTTATGGTGCGAAGAGCGTGGAGATGGCGCATGAGTTGATGGCCATCGAAACGCTGTTGATCATGGATGATTTGTTTGAGAGGGCAGAAATCGGGACAAGGCATAAGTATGTAGGGTTGGTGAAGTCGGTGAAAGAGGCGGGAGGGAAGGCGATTGTGTTTTCGGCAAAACATGTGGCCAGAGTTGAGCTGGCCACCACCGGCATTGCCACAACTTTGAGGTTTCCAATGCCTGATCTTGATGATGGTTTCGTAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

171

Amino Acids

18.97

Weight (kDa)

6.53

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AciI CCGC 2 cut(s) 158, 392
AclWI GGATC 1 cut(s) 252
AcoI YGGCCR 3 cut(s) 294, 429, 444
AcsI RAATTY 1 cut(s) 166
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 2 cut(s) 49, 55
AluBI AGCT 2 cut(s) 132, 442
AluI AGCT 2 cut(s) 132, 442
AlwI GGATC 1 cut(s) 252
AoxI GGCC 3 cut(s) 294, 429, 444
ApeKI GCWGC 2 cut(s) 129, 188
ApoI RAATTY 1 cut(s) 166
Asp700I GAANNNNTTC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 385, 394
BalI TGGCCA 3 cut(s) 296, 431, 446
BbsI GAAGAC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 141, 175
BccI CCATC 5 cut(s) 187, 271, 286, 305, 491
BclI TGATCA 2 cut(s) 171, 312
BisI GCNGC 2 cut(s) 130, 189
BlsI GCNGC 2 cut(s) 131, 190
BoxI GACNNNNGTC 1 cut(s) 15
BpiI GAAGAC 1 cut(s) 15
BsaBI GATNNNNATC 2 cut(s) 197, 296
BsaJI CCNNGG 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bse118I RCCGGY 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 2 cut(s) 197, 296
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 1 cut(s) 325
BseJI GATNNNNATC 2 cut(s) 197, 296
BseMI GCAATG 1 cut(s) 456
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 141, 175
BshFI GGCC 3 cut(s) 296, 431, 446
BsiSI CCGG 1 cut(s) 453
BslFI GGGAC 1 cut(s) 361
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 3 cut(s) 296, 431, 446
Bsp143I GATC 5 cut(s) 171, 198, 244, 312, 487
BspACI CCGC 2 cut(s) 158, 392
BspANI GGCC 3 cut(s) 296, 431, 446
BspHI TCATGA 1 cut(s) 162
BspPI GGATC 1 cut(s) 252
BspQI GCTCTTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 5 cut(s) 171, 198, 244, 312, 487
BssT1I CCWWGG 1 cut(s) 183
Bst6I CTCTTC 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 134, 444
BstF5I GGATG 1 cut(s) 325
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 5 cut(s) 174, 201, 247, 315, 490
BstMBI GATC 5 cut(s) 171, 198, 244, 312, 487
BstNSI RCATGY 1 cut(s) 428
BstPAI GACNNNNGTC 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 141, 175
BstV2I GAAGAC 1 cut(s) 15
BsuRI GGCC 3 cut(s) 296, 431, 446
BtgZI GCGATG 1 cut(s) 206
BtsCI GGATG 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 134, 444
CciI TCATGA 1 cut(s) 162
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 452
CspCI CAANNNNNGTGG 4 cut(s) 408, 439, 443, 474
CviAII CATG 7 cut(s) 137, 149, 163, 226, 284, 316, 425
CviJI RGCY 7 cut(s) 132, 188, 206, 296, 431, 442, 446
CviKI_1 RGCY 7 cut(s) 132, 188, 206, 296, 431, 442, 446
DpnI GATC 5 cut(s) 173, 200, 246, 314, 489
DpnII GATC 5 cut(s) 171, 198, 244, 312, 487
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 3 cut(s) 294, 429, 444
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 7 cut(s) 140, 152, 166, 229, 287, 319, 428
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 1 cut(s) 361
FatI CATG 7 cut(s) 136, 148, 162, 225, 283, 315, 424
FauI CCCGC 1 cut(s) 385
FbaI TGATCA 2 cut(s) 171, 312
Fnu4HI GCNGC 2 cut(s) 130, 189
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 2 cut(s) 130, 189
GlaI GCGC 1 cut(s) 282
GluI GCNGC 2 cut(s) 130, 189
HaeIII GGCC 3 cut(s) 296, 431, 446
HapII CCGG 1 cut(s) 453
HhaI GCGC 1 cut(s) 283
Hin1II CATG 7 cut(s) 140, 152, 166, 229, 287, 319, 428
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HpaII CCGG 1 cut(s) 453
HphI GGTGA 2 cut(s) 385, 394
Hpy188I TCNGA 2 cut(s) 223, 244
Hpy188III TCNNGA 3 cut(s) 163, 346, 491
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 394
HpyCH4V TGCA 2 cut(s) 136, 148
Hsp92II CATG 7 cut(s) 140, 152, 166, 229, 287, 319, 428
HspAI GCGC 1 cut(s) 281
Ksp22I TGATCA 2 cut(s) 171, 312
Kzo9I GATC 5 cut(s) 171, 198, 244, 312, 487
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 5 cut(s) 187, 428, 445, 466, 498
Lsp1109I GCAGC 2 cut(s) 141, 175
MaeIII GTNAC 1 cut(s) 505
MalI GATC 5 cut(s) 173, 200, 246, 314, 489
MboI GATC 5 cut(s) 171, 198, 244, 312, 487
MboII GAAGA 2 cut(s) 20, 277
MlsI TGGCCA 3 cut(s) 296, 431, 446
MluCI AATT 3 cut(s) 118, 166, 238
MluNI TGGCCA 3 cut(s) 296, 431, 446
MnlI CCTC 7 cut(s) 28, 36, 133, 327, 382, 389, 465
Mox20I TGGCCA 3 cut(s) 296, 431, 446
MroXI GAANNNNTTC 1 cut(s) 12
MscI TGGCCA 3 cut(s) 296, 431, 446
MseI TTAA 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 360
Msp20I TGGCCA 3 cut(s) 296, 431, 446
MspI CCGG 1 cut(s) 453
NdeII GATC 5 cut(s) 171, 198, 244, 312, 487
NlaIII CATG 7 cut(s) 140, 152, 166, 229, 287, 319, 428
NspI RCATGY 1 cut(s) 428
PagI TCATGA 1 cut(s) 162
PciI ACATGT 1 cut(s) 424
PciSI GCTCTTC 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 12
PkrI GCNGC 2 cut(s) 131, 190
PscI ACATGT 1 cut(s) 424
PshAI GACNNNNGTC 1 cut(s) 15
RseI CAYNNNNRTG 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 2 cut(s) 130, 189
Sau3AI GATC 5 cut(s) 171, 198, 244, 312, 487
SetI ASST 5 cut(s) 47, 134, 144, 444, 476
SmiMI CAYNNNNRTG 1 cut(s) 360
Sse9I AATT 3 cut(s) 118, 166, 238
SsiI CCGC 2 cut(s) 158, 392
StyI CCWWGG 1 cut(s) 183
TaqI TCGA 2 cut(s) 233, 300
TasI AATT 3 cut(s) 118, 166, 238
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseI GCWGC 2 cut(s) 129, 188
TspDTI ATGAA 2 cut(s) 17, 179
XapI RAATTY 1 cut(s) 166
XceI RCATGY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 2 cut(s) 190, 439
XmnI GAANNNNTTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.