Rmu_sc0006582.1_g000016
ERF Family

May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006582.1
OG0001369
Physical Location & Seq
Reverse (-)
101914 .. 102787
874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006582.1_g000016.1.cds

Sequence Viewer

Length: 723 bp
atggtcaagatcctccccgaagacctcgacgacgtctggtttgttttcaacctcatctcccccggtgatgtcatcatcgccgccaccacccgcagcgtcaaatacagctcccgcatgaaggtcattctcgaaatctcgatcaccgccgtggactaccacaaggactcaccaagactctgctccagggtggaaactcactccaaccctaacaagtacttcgagaacattttccaggctttcgtgaagcacgttgatctgagcgtcgtacggtgcgtcgtaatagggagtcagggaaacgcgaaggacgagttccgtcgttttatgattgcggaggcgaataagttgaaggtcaggtcagtaaagaacaacaagcaacgcattgtcgtttttaacacgaggagcaaagggaaagacgggaacagtagtttgcttgaggttttgcatgacagtaccgtgatgaacttggtcagagacaccaaggctgtgatggagatcaaggcttttaaggagttttcggatttgctaacaacggatttggaccgagtgtgttatggtaccaagaatgtggagatggcgcatgagttgaaggccaatgaaacgctattgattaccgatgacttgtatgagagtgtacagattgagacgaccagagggtttgtggagtcggagaaaaagacaggagggaaggcgtttgagttttcgtccgagcatgtatctagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

240

Amino Acids

27.22

Weight (kDa)

7.02

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
Acc65I GGTACC 1 cut(s) 554
AccB1I GGYRCC 1 cut(s) 554
AccII CGCG 1 cut(s) 299
AciI CCGC 5 cut(s) 81, 91, 112, 144, 329
AclWI GGATC 1 cut(s) 4
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 5 cut(s) 215, 267, 451, 556, 633
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 3 cut(s) 49, 346, 586
AjnI CCWGG 2 cut(s) 182, 231
AleI CACNNNNGTG 1 cut(s) 146
AluBI AGCT 2 cut(s) 108, 720
AluI AGCT 2 cut(s) 108, 720
Alw26I GTCTC 2 cut(s) 465, 635
AlwI GGATC 1 cut(s) 4
AoxI GGCC 1 cut(s) 588
ApeKI GCWGC 1 cut(s) 93
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
Asp700I GAANNNNTTC 1 cut(s) 227
Asp718I GGTACC 1 cut(s) 554
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 1 cut(s) 538
AsuC2I CCSGG 1 cut(s) 63
AsuHPI GGTGA 3 cut(s) 77, 133, 159
AvaII GGWCC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 554
BauI CACGAG 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 105
BccI CCATC 2 cut(s) 481, 565
BceAI ACGGC 1 cut(s) 131
BciT130I CCWGG 2 cut(s) 184, 233
BcnI CCSGG 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 465, 635
BfaI CTAG 2 cut(s) 717, 721
BisI GCNGC 2 cut(s) 81, 94
BlsI GCNGC 2 cut(s) 82, 95
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 3 cut(s) 63, 184, 233
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 3 cut(s) 63, 184, 233
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 63
BsaBI GATNNNNATC 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 4 cut(s) 61, 147, 183, 477
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse8I GATNNNNATC 1 cut(s) 491
BseBI CCWGG 2 cut(s) 184, 233
BseDI CCNNGG 4 cut(s) 61, 147, 183, 477
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 412
BseXI GCAGC 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 299
BshFI GGCC 1 cut(s) 590
BshNI GGYRCC 1 cut(s) 554
BsiSI CCGG 1 cut(s) 63
BsiWI CGTACG 1 cut(s) 265
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 2 cut(s) 465, 635
BsmBI CGTCTC 1 cut(s) 635
BsnI GGCC 1 cut(s) 590
Bsp1407I TGTACA 1 cut(s) 631
Bsp143I GATC 4 cut(s) 9, 138, 253, 492
BspACI CCGC 5 cut(s) 81, 91, 112, 144, 329
BspANI GGCC 1 cut(s) 590
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 1 cut(s) 556
BspPI GGATC 1 cut(s) 4
BspT107I GGYRCC 1 cut(s) 554
BsrGI TGTACA 1 cut(s) 631
BssECI CCNNGG 4 cut(s) 61, 147, 183, 477
BssMI GATC 4 cut(s) 9, 138, 253, 492
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 1 cut(s) 394
BssT1I CCWWGG 1 cut(s) 477
Bst2BI CACGAG 1 cut(s) 394
Bst2UI CCWGG 2 cut(s) 184, 233
Bst4CI ACNGT 4 cut(s) 270, 422, 449, 454
BstACI GRCGYC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 631
BstDEI CTNAG 1 cut(s) 257
BstDSI CCRYGG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 299
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 4 cut(s) 12, 141, 256, 495
BstMAI GTCTC 2 cut(s) 465, 635
BstMBI GATC 4 cut(s) 9, 138, 253, 492
BstNI CCWGG 2 cut(s) 184, 233
BstNSI RCATGY 1 cut(s) 713
BstSCI CCNGG 3 cut(s) 61, 182, 231
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 565
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 1 cut(s) 590
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 61
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 538
CseI GACGC 3 cut(s) 85, 250, 262
Csp6I GTAC 5 cut(s) 214, 266, 450, 555, 632
CviAII CATG 4 cut(s) 115, 443, 578, 710
CviJI RGCY 6 cut(s) 108, 236, 482, 500, 590, 720
CviKI_1 RGCY 6 cut(s) 108, 236, 482, 500, 590, 720
CviQI GTAC 5 cut(s) 214, 266, 450, 555, 632
DdeI CTNAG 1 cut(s) 257
DpnI GATC 4 cut(s) 11, 140, 255, 494
DpnII GATC 4 cut(s) 9, 138, 253, 492
Eco130I CCWWGG 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 538
EcoRII CCWGG 2 cut(s) 182, 231
EcoT14I CCWWGG 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 477
Esp3I CGTCTC 1 cut(s) 635
FaeI CATG 4 cut(s) 118, 446, 581, 713
FaiI YATR 7 cut(s) 116, 323, 444, 552, 579, 624, 711
FatI CATG 4 cut(s) 114, 442, 577, 709
FauI CCCGC 2 cut(s) 98, 119
Fnu4HI GCNGC 2 cut(s) 81, 94
Fsp4HI GCNGC 2 cut(s) 81, 94
FspBI CTAG 2 cut(s) 717, 721
GlaI GCGC 1 cut(s) 576
GluI GCNGC 2 cut(s) 81, 94
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 590
HapII CCGG 1 cut(s) 63
HgaI GACGC 3 cut(s) 85, 250, 262
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 4 cut(s) 118, 446, 581, 713
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HinfI GANTC 4 cut(s) 164, 174, 286, 662
HpaII CCGG 1 cut(s) 63
HphI GGTGA 3 cut(s) 77, 133, 159
Hpy166II GTNNAC 2 cut(s) 151, 632
Hpy188I TCNGA 5 cut(s) 258, 470, 517, 667, 706
Hpy188III TCNNGA 5 cut(s) 7, 128, 136, 220, 241
Hpy8I GTNNAC 2 cut(s) 151, 632
Hpy99I CGWCG 5 cut(s) 32, 35, 266, 278, 318
HpyAV CCTTC 5 cut(s) 112, 295, 340, 580, 679
HpyCH4III ACNGT 4 cut(s) 270, 422, 449, 454
HpyCH4IV ACGT 2 cut(s) 33, 249
HpyCH4V TGCA 1 cut(s) 442
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 2 cut(s) 33, 249
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 4 cut(s) 118, 446, 581, 713
HspAI GCGC 1 cut(s) 575
KpnI GGTACC 1 cut(s) 558
Kzo9I GATC 4 cut(s) 9, 138, 253, 492
LmnI GCTCC 3 cut(s) 113, 185, 399
Lsp1109I GCAGC 1 cut(s) 105
MaeI CTAG 2 cut(s) 717, 721
MaeII ACGT 2 cut(s) 33, 249
MalI GATC 4 cut(s) 11, 140, 255, 494
MboI GATC 4 cut(s) 9, 138, 253, 492
MboII GAAGA 1 cut(s) 32
MflI RGATCY 1 cut(s) 9
MlyI GAGTC 4 cut(s) 158, 168, 295, 671
MmeI TCCRAC 2 cut(s) 225, 645
MnlI CCTC 8 cut(s) 23, 35, 62, 325, 390, 427, 644, 674
MroXI GAANNNNTTC 1 cut(s) 227
MseI TTAA 2 cut(s) 390, 504
MslI CAYNNNNRTG 1 cut(s) 146
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 3 cut(s) 63, 184, 233
MvaI CCWGG 2 cut(s) 184, 233
MvnI CGCG 1 cut(s) 299
NciI CCSGG 1 cut(s) 63
NdeII GATC 4 cut(s) 9, 138, 253, 492
NlaIII CATG 4 cut(s) 118, 446, 581, 713
NlaIV GGNNCC 1 cut(s) 556
NspI RCATGY 1 cut(s) 713
OliI CACNNNNGTG 1 cut(s) 146
PcsI WCGNNNNNNNCGW 3 cut(s) 246, 270, 303
PdmI GAANNNNTTC 1 cut(s) 227
Pfl23II CGTACG 1 cut(s) 265
PflFI GACNNNGTC 1 cut(s) 32
PkrI GCNGC 2 cut(s) 82, 95
PleI GAGTC 4 cut(s) 158, 168, 294, 670
PpsI GAGTC 4 cut(s) 158, 168, 294, 670
Psp6I CCWGG 2 cut(s) 182, 231
PspGI CCWGG 2 cut(s) 182, 231
PspLI CGTACG 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 538
PsuI RGATCY 1 cut(s) 9
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 5 cut(s) 215, 267, 451, 556, 633
RsaNI GTAC 5 cut(s) 214, 266, 450, 555, 632
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 2 cut(s) 390, 504
SatI GCNGC 2 cut(s) 81, 94
Sau3AI GATC 4 cut(s) 9, 138, 253, 492
Sau96I GGNCC 1 cut(s) 538
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 4 cut(s) 158, 168, 295, 671
ScrFI CCNGG 3 cut(s) 63, 184, 233
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SsiI CCGC 5 cut(s) 81, 91, 112, 144, 329
SspMI CTAG 2 cut(s) 717, 721
StyD4I CCNGG 3 cut(s) 61, 182, 231
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 4 cut(s) 270, 422, 449, 454
TaiI ACGT 2 cut(s) 36, 252
TaqI TCGA 4 cut(s) 27, 129, 137, 219
TaqII GACCGA 1 cut(s) 555
TatI WGTACW 2 cut(s) 213, 631
TauI GCSGC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 390, 504
Tru9I TTAA 2 cut(s) 390, 504
TseI GCWGC 1 cut(s) 93
TspDTI ATGAA 3 cut(s) 131, 473, 609
TspGWI ACGGA 2 cut(s) 302, 545
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 538
XceI RCATGY 1 cut(s) 713
XcmI CCANNNNNNNNNTGG 2 cut(s) 484, 655
XmnI GAANNNNTTC 1 cut(s) 227
XspI CTAG 2 cut(s) 717, 721
ZraI GACGTC 1 cut(s) 34
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.