MD04G1241100.v1.1

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011162
Physical Location & Seq
Forward (+)
31828587 .. 31832485
3899 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1241100.v1.1.491

Sequence Viewer

Length: 2169 bp
ATGGCGGACGTCATGACGTACAATGACATAGAGGCGACGGCGGCCCGAATCGGAATCGACCTCTCGCAGCTCGACCTCGATTCCATCCGCCTCCCTGCCGGCGAAGACTTTGGCATTATCAGTGATGATGAGGATATATACAATGTGGACAATTCGGAGTTGGACGCAGGGTTTAGCAACATAATTGTTGTTGATAACCTCCCTGTTGTTCCTTTGGAAAAGTTTGAGAAGCTCGAAAACGTCATTCGTAAAATCTATAGCCAGATTGGTGTGATCAAAGACGACGGCTTTTGGATGCCTCTTGACCCCGAAACCAATAAAACCTTGGGGTATTGCTTCCTTGAGTTTAATACTCCTCAGGAAGCTGAGCTTGCCAAAGAGAGGACTCATGGATACAAGCTGGATCGATCACATATTTTTGTTGTCAACATTTTTAACGACTTTGATCGGTTCATGAGTGTTCCGGATCAATGGGCCCCTCCAGAAAGTAAACCGTATACACCAGGGGAAAACCTTCAACAGTGGCTAACCGACGAAAAGGCCAGGGACCAACTGGTCATTCGTTATGGTGCGGACACGGAAGTGCTATGGAACGATGCAAGGCACCTGAAGCCGGAACCGTTCTACAAACGCACTTTCTGGACTGAGAGCTTCGTGCAGTGGTCCCCTCTGGGGACTTACCTGGCCACCGTTCATAGGCAGGGTGCTGCAGTCTGGGGAGGCGCCTCTACGTTCAATCGTCTGATGCGTTATGCTCATTCCCAGGTTCGACTGATTGATTTCTCACCTGGTGAGAAATACTTGGTGACATACAGCAGCCATGAACCAAGCAATCCTCGTGATGCAAATAGGGTCGTGATAAACATTTTTGATGTGAGGACCGGAAAGGTAATGAGAGATTTTAAAGGAAGCCCAGATGATTTTGCTATTGGAGGAGCTGGAGGTGTTGCTGGGGTGTCTTGGCCTGTTTTCAAATGGGCCGGGGGAAAAGATGACAAGTACTTTGCCAGGATGGGAAAAAATGTTATTTCTGTATACGAGACGGAAACATTTTCTCTTGTAGACAAAAAGTCAATGAAGGTGGAAAATGTCATGGACTTCAGTTGGTCACCAACTGATCCGATTCTTGCACTATTTGTTCCAGAACTGGGTGGTGGCAACCAGCCTGCTAGGGTGAGTCTTGTTCAAATCCCTGCTAAGGAGGAGTTGAGGCAGAAGAATCTTTTCAGCGTCAGCGATTGCAAAATGTATTGGCAGAGCAATGGGGAGTATCTAGCTGTTAAAGTCGACCGCTACACCAAAACAAAGAAAAGCACATACACTGGATTTGAGCTTTTCAGAATCAAGGAGCGGGACATTCCTATCGAAGTCTTGGAATTGGAGAATAAGAATGACAAAATTATTGCATTCGCTTGGGAGCCTAAGGGCCACCGGTTCGCAGTAATTCATTGTGATAACACGAACGCGAATAACAACCCAAGGCCCGACATAAGTTTCTACTCCATGCGAAGTGCTCACAATACTGGCCGTGTTTCAAAGCTCACTACTCTCAAGGGAAAGCAGGCAAATGCCCTTTACTGGTCACCTACCGGCCACTTCATCATTCTGGCAGGATTGAAAGGTTTTAATGGGCAGCTAGAGTTTTACAATGTAGATGAGCTGGAAACCATGGCCACCGCTGAACATTTCATGGCGACGGATATCGAATGGGATCCGACCGGAAGATACGTAGCTACTACAGTGACCTCAGTCCATGAGATGGAGAACGGCTTCAACATCTGGACATTCAATGGCAAGCTACTTTATCGGATACTAAAGGATCATTTCTTTCAGTTCTTATGGCGCCCAAGGCCACCATCATTCTTGACTCCTGAGAAAGAGGAGGAGATTGCAAAGAACTTGAAGAAGTACAGCAAGAAGTATGAGGCAGAGGACCAGGATGTTTCAATCTTCTTGAGCGAGCAGGATCGCGAGAAAAGAAGAATGTTGAAGGAGGAATGGGAGAAGTGGGTGGCTCAGTGGAAGCAGAAGCACGAAGAAGAGAAAGAGGTGAGGAAAAATCTTAGGGACGGAGAAGCAAGTGATGAGGAAGAGGAGTACGAGGCTAAAGCTATTGAGGTCGAAGAGGTGATAAACCAGGTAGAGGAGGTTGTTGCTTTCGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

723

Amino Acids

83.31

Weight (kDa)

5.13

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 79 - 136 2.3e-06 RNA recognition motif
eIF2A PF08662 406 - 612 5.9e-58 Eukaryotic translation initiation factor eIF2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25780 AT5G27640 AT5G27640 AT5G27640
fragaria_vesca FvH4_6g00800
malus_domestica MD04G1241100.v1.1 MD12G1258100.v1.1
prunus_persica Prupe.6G358600_v2.0.a1
pyrus_communis pycom04g21280 pycom12g23670
rosa_chinensis RchiOBHm_Chr3g0448281
rosa_laevigata RLG00000025930
rosa_multiflora Rmu_sc0005582.1_g000005
rosa_roxburghii Rroxscaffold_6G00426430
rosa_rugosa Rorug02G0610000
rosa_samantha Rh3AG009600 Rh3BG009200 Rh3CG009200 Rh3DG009800
rosa_wichuraiana Rw3G000780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AatII GACGTC 1 cut(s) 12
AccB1I GGYRCC 3 cut(s) 603, 722, 1842
AccB7I CCANNNNNTGG 1 cut(s) 1759
AccBSI CCGCTC 1 cut(s) 1351
AccI GTMKAC 4 cut(s) 497, 1035, 1062, 1287
AccII CGCG 2 cut(s) 1466, 1971
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 7 cut(s) 5, 41, 88, 572, 1291, 1351, 1677
AclWI GGATC 7 cut(s) 411, 474, 1112, 1706, 1719, 1827, 1974
AcoI YGGCCR 4 cut(s) 684, 1525, 1591, 1671
AcuI CTGAAG 2 cut(s) 629, 1084
AcyI GRCGYC 3 cut(s) 9, 723, 1843
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 4 cut(s) 20, 1001, 1910, 2099
AfiI CCNNNNNNNGG 9 cut(s) 381, 613, 672, 696, 1148, 1198, 1578, 1759, 2143
AgeI ACCGGT 1 cut(s) 1431
AhdI GACNNNNNGTC 1 cut(s) 1069
AjnI CCWGG 8 cut(s) 502, 542, 681, 762, 787, 1007, 1935, 2136
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AleI CACNNNNGTG 1 cut(s) 581
Alw21I GWGCWC 1 cut(s) 1516
Alw26I GTCTC 1 cut(s) 1034
AlwI GGATC 7 cut(s) 411, 474, 1112, 1706, 1719, 1827, 1974
Aor13HI TCCGGA 1 cut(s) 463
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 4 cut(s) 67, 707, 816, 1633
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
AsiGI ACCGGT 1 cut(s) 1431
Asp700I GAANNNNTTC 3 cut(s) 513, 1223, 1769
AspLEI GCGC 2 cut(s) 725, 1845
AsuC2I CCSGG 1 cut(s) 982
AsuHPI GGTGA 8 cut(s) 777, 803, 817, 1101, 1186, 1575, 2062, 2140
AsuII TTCGAA 1 cut(s) 2160
AvaII GGWCC 4 cut(s) 547, 663, 879, 1933
AxyI CCTNAGG 2 cut(s) 357, 1422
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 2 cut(s) 686, 1673
BamHI GGATCC 1 cut(s) 1711
BanI GGYRCC 3 cut(s) 603, 722, 1842
BanII GRGCYC 1 cut(s) 478
BauI CACGAG 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 1516
BbvI GCAGC 4 cut(s) 79, 694, 828, 1645
BccI CCATC 4 cut(s) 92, 1006, 1753, 1864
BceAI ACGGC 4 cut(s) 54, 301, 1512, 1783
BciT130I CCWGG 8 cut(s) 504, 544, 683, 764, 789, 1009, 1937, 2138
BciVI GTATCC 2 cut(s) 386, 1803
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 1 cut(s) 982
BcoDI GTCTC 1 cut(s) 1034
BfaI CTAG 3 cut(s) 1170, 1274, 1637
BfmI CTRYAG 3 cut(s) 256, 708, 1737
BfoI RGCGCY 2 cut(s) 726, 1846
BfuI GTATCC 2 cut(s) 386, 1803
BisI GCNGC 5 cut(s) 42, 68, 708, 817, 1634
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 5 cut(s) 43, 69, 709, 818, 1635
BmcAI AGTACT 1 cut(s) 1001
Bme1390I CCNGG 9 cut(s) 504, 544, 683, 764, 789, 982, 1009, 1937, 2138
Bme18I GGWCC 4 cut(s) 547, 663, 879, 1933
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmrFI CCNGG 9 cut(s) 504, 544, 683, 764, 789, 982, 1009, 1937, 2138
BmrI ACTGGG 1 cut(s) 1157
BmsI GCATC 4 cut(s) 285, 586, 735, 832
BmuI ACTGGG 1 cut(s) 1157
BoxI GACNNNNGTC 1 cut(s) 1748
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 2 cut(s) 465, 960
Bpu10I CCTNAGC 1 cut(s) 1197
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 1 cut(s) 2160
BpuEI CTTGAG 3 cut(s) 362, 1535, 1975
BpuMI CCSGG 1 cut(s) 982
Bsa29I ATCGAT 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 1729
BsaHI GRCGYC 3 cut(s) 9, 723, 1843
BsaJI CCNNGG 8 cut(s) 324, 503, 543, 762, 981, 1478, 1668, 1847
BsaWI WCCGGW 4 cut(s) 463, 881, 1431, 1718
Bsc4I CCNNNNNNNGG 9 cut(s) 381, 613, 672, 696, 1148, 1198, 1578, 1759, 2143
Bse118I RCCGGY 3 cut(s) 98, 1431, 1589
Bse1I ACTGG 5 cut(s) 558, 1152, 1327, 1528, 1583
Bse21I CCTNAGG 2 cut(s) 357, 1422
Bse3DI GCAATG 1 cut(s) 1267
BseAI TCCGGA 1 cut(s) 463
BseBI CCWGG 8 cut(s) 504, 544, 683, 764, 789, 1009, 1937, 2138
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 8 cut(s) 324, 503, 543, 762, 981, 1478, 1668, 1847
BseGI GGATG 4 cut(s) 84, 300, 1017, 1945
BseLI CCNNNNNNNGG 9 cut(s) 381, 613, 672, 696, 1148, 1198, 1578, 1759, 2143
BseMI GCAATG 1 cut(s) 1267
BseMII CTCAG 6 cut(s) 357, 371, 636, 1761, 1863, 2030
BseNI ACTGG 5 cut(s) 558, 1152, 1327, 1528, 1583
BseRI GAGGAG 7 cut(s) 345, 948, 1217, 1895, 1898, 2108, 2159
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 4 cut(s) 79, 694, 828, 1645
BseYI CCCAGC 1 cut(s) 950
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 2 cut(s) 1466, 1971
Bsh1285I CGRYCG 2 cut(s) 1291, 1719
BshNI GGYRCC 3 cut(s) 603, 722, 1842
BshTI ACCGGT 1 cut(s) 1431
BshVI ATCGAT 1 cut(s) 406
BsiEI CGRYCG 2 cut(s) 1291, 1719
BsiHKAI GWGCWC 1 cut(s) 1516
BsiSI CCGG 8 cut(s) 99, 464, 614, 882, 981, 1432, 1590, 1719
BslFI GGGAC 5 cut(s) 560, 649, 688, 1367, 2081
BslI CCNNNNNNNGG 9 cut(s) 381, 613, 672, 696, 1148, 1198, 1578, 1759, 2143
BsmAI GTCTC 1 cut(s) 1034
BsmBI CGTCTC 1 cut(s) 1034
BsmFI GGGAC 5 cut(s) 560, 649, 688, 1367, 2081
BsmI GAATGC 1 cut(s) 1406
Bsp119I TTCGAA 1 cut(s) 2160
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 478, 1516
Bsp13I TCCGGA 1 cut(s) 463
Bsp143I GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
Bsp1720I GCTNAGC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 1668
Bsp68I TCGCGA 1 cut(s) 1971
BspACI CCGC 7 cut(s) 5, 41, 88, 572, 1291, 1351, 1677
BspCNI CTCAG 6 cut(s) 358, 370, 637, 1760, 1864, 2029
BspDI ATCGAT 1 cut(s) 406
BspEI TCCGGA 1 cut(s) 463
BspFNI CGCG 2 cut(s) 1466, 1971
BspHI TCATGA 2 cut(s) 12, 453
BspMAI CTGCAG 1 cut(s) 712
BspPI GGATC 7 cut(s) 411, 474, 1112, 1706, 1719, 1827, 1974
BspT104I TTCGAA 1 cut(s) 2160
BspT107I GGYRCC 3 cut(s) 603, 722, 1842
BsrBI CCGCTC 1 cut(s) 1351
BsrDI GCAATG 1 cut(s) 1267
BsrFI RCCGGY 3 cut(s) 98, 1431, 1589
BsrI ACTGG 5 cut(s) 558, 1152, 1327, 1528, 1583
BssAI RCCGGY 3 cut(s) 98, 1431, 1589
BssECI CCNNGG 8 cut(s) 324, 503, 543, 762, 981, 1478, 1668, 1847
BssMI GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
BssNAI GTATAC 2 cut(s) 498, 1036
BssNI GRCGYC 3 cut(s) 9, 723, 1843
BssSI CACGAG 1 cut(s) 837
BssT1I CCWWGG 4 cut(s) 324, 1478, 1668, 1847
Bst1107I GTATAC 2 cut(s) 498, 1036
Bst2BI CACGAG 1 cut(s) 837
Bst2UI CCWGG 8 cut(s) 504, 544, 683, 764, 789, 1009, 1937, 2138
Bst4CI ACNGT 5 cut(s) 495, 522, 621, 691, 1741
Bst6I CTCTTC 3 cut(s) 2034, 2085, 2118
BstACI GRCGYC 3 cut(s) 9, 723, 1843
BstBAI YACGTR 1 cut(s) 1729
BstBI TTCGAA 1 cut(s) 2160
BstC8I GCNNGC 6 cut(s) 100, 372, 1167, 1563, 1796, 1961
BstDEI CTNAG 9 cut(s) 357, 366, 645, 1197, 1422, 1747, 1872, 2016, 2063
BstDSI CCRYGG 1 cut(s) 1668
BstEII GGTNACC 2 cut(s) 1107, 1581
BstF5I GGATG 4 cut(s) 84, 300, 1017, 1945
BstFNI CGCG 2 cut(s) 1466, 1971
BstH2I RGCGCY 2 cut(s) 726, 1846
BstHHI GCGC 2 cut(s) 725, 1845
BstKTI GATC 9 cut(s) 276, 406, 410, 448, 469, 1120, 1714, 1822, 1969
BstMAI GTCTC 1 cut(s) 1034
BstMBI GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
BstMCI CGRYCG 2 cut(s) 1291, 1719
BstMWI GCNNNNNNNGC 4 cut(s) 41, 371, 610, 1849
BstNI CCWGG 8 cut(s) 504, 544, 683, 764, 789, 1009, 1937, 2138
BstPAI GACNNNNGTC 1 cut(s) 1748
BstPI GGTNACC 2 cut(s) 1107, 1581
BstSCI CCNGG 9 cut(s) 502, 542, 681, 762, 787, 980, 1007, 1935, 2136
BstSFI CTRYAG 3 cut(s) 256, 708, 1737
BstSLI GKGCMC 1 cut(s) 478
BstSNI TACGTA 1 cut(s) 1729
BstUI CGCG 2 cut(s) 1466, 1971
BstV1I GCAGC 4 cut(s) 79, 694, 828, 1645
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 1711
BstYI RGATCY 1 cut(s) 1711
BstZ17I GTATAC 2 cut(s) 498, 1036
Bsu15I ATCGAT 1 cut(s) 406
Bsu36I CCTNAGG 2 cut(s) 357, 1422
BsuI GTATCC 2 cut(s) 386, 1803
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 1668
BtsCI GGATG 4 cut(s) 84, 300, 1017, 1945
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 6 cut(s) 127, 527, 665, 1320, 1746, 2024
BtuMI TCGCGA 1 cut(s) 1971
Cac8I GCNNGC 6 cut(s) 100, 372, 1167, 1563, 1796, 1961
CciI TCATGA 2 cut(s) 12, 453
CfoI GCGC 2 cut(s) 725, 1845
Cfr10I RCCGGY 3 cut(s) 98, 1431, 1589
ClaI ATCGAT 1 cut(s) 406
CseI GACGC 2 cut(s) 173, 1219
CsiI ACCWGGT 2 cut(s) 787, 2136
Csp6I GTAC 4 cut(s) 19, 1000, 1909, 2098
CspAI ACCGGT 1 cut(s) 1431
CspCI CAANNNNNGTGG 2 cut(s) 1062, 1097
CviAII CATG 9 cut(s) 13, 389, 454, 821, 1093, 1504, 1669, 1690, 1754
CviQI GTAC 4 cut(s) 19, 1000, 1909, 2098
DdeI CTNAG 9 cut(s) 357, 366, 645, 1197, 1422, 1747, 1872, 2016, 2063
DinI GGCGCC 2 cut(s) 724, 1844
DpnI GATC 9 cut(s) 275, 405, 409, 447, 468, 1119, 1713, 1821, 1968
DpnII GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
DraI TTTAAA 1 cut(s) 904
DraIII CACNNNGTG 1 cut(s) 791
DrdI GACNNNNNNGTC 1 cut(s) 554
DriI GACNNNNNGTC 1 cut(s) 1069
DseDI GACNNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 4 cut(s) 684, 1525, 1591, 1671
Eam1104I CTCTTC 3 cut(s) 2034, 2085, 2118
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 3 cut(s) 2034, 2085, 2118
EciI GGCGGA 2 cut(s) 20, 77
Eco105I TACGTA 1 cut(s) 1729
Eco130I CCWWGG 4 cut(s) 324, 1478, 1668, 1847
Eco24I GRGCYC 1 cut(s) 478
Eco32I GATATC 1 cut(s) 1702
Eco47I GGWCC 4 cut(s) 547, 663, 879, 1933
Eco57I CTGAAG 2 cut(s) 629, 1084
Eco81I CCTNAGG 2 cut(s) 357, 1422
Eco91I GGTNACC 2 cut(s) 1107, 1581
EcoO109I RGGNCCY 1 cut(s) 475
EcoO65I GGTNACC 2 cut(s) 1107, 1581
EcoRII CCWGG 8 cut(s) 502, 542, 681, 762, 787, 1007, 1935, 2136
EcoRV GATATC 1 cut(s) 1702
EcoT14I CCWWGG 4 cut(s) 324, 1478, 1668, 1847
EcoT38I GRGCYC 1 cut(s) 478
EgeI GGCGCC 2 cut(s) 724, 1844
EheI GGCGCC 2 cut(s) 724, 1844
ErhI CCWWGG 4 cut(s) 324, 1478, 1668, 1847
Esp3I CGTCTC 1 cut(s) 1034
FaeI CATG 9 cut(s) 16, 392, 457, 824, 1096, 1507, 1672, 1693, 1757
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 5 cut(s) 560, 649, 688, 1367, 2081
FatI CATG 9 cut(s) 12, 388, 453, 820, 1092, 1503, 1668, 1689, 1753
FauI CCCGC 1 cut(s) 1344
FbaI TGATCA 1 cut(s) 273
FblI GTMKAC 4 cut(s) 497, 1035, 1062, 1287
Fnu4HI GCNGC 5 cut(s) 42, 68, 708, 817, 1634
FokI GGATG 4 cut(s) 71, 307, 1024, 1952
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 5 cut(s) 42, 68, 708, 817, 1634
FspBI CTAG 3 cut(s) 1170, 1274, 1637
GlaI GCGC 2 cut(s) 724, 1844
GluI GCNGC 5 cut(s) 42, 68, 708, 817, 1634
GsaI CCCAGC 1 cut(s) 954
GsuI CTGGAG 2 cut(s) 465, 960
HaeII RGCGCY 2 cut(s) 726, 1846
HapII CCGG 8 cut(s) 99, 464, 614, 882, 981, 1432, 1590, 1719
HgaI GACGC 2 cut(s) 173, 1219
HhaI GCGC 2 cut(s) 725, 1845
Hin1I GRCGYC 3 cut(s) 9, 723, 1843
Hin1II CATG 9 cut(s) 16, 392, 457, 824, 1096, 1507, 1672, 1693, 1757
Hin6I GCGC 2 cut(s) 723, 1843
HinP1I GCGC 2 cut(s) 723, 1843
HincII GTYRAC 2 cut(s) 427, 1288
HindII GTYRAC 2 cut(s) 427, 1288
HinfI GANTC 9 cut(s) 48, 54, 80, 385, 1123, 1177, 1219, 1341, 1867
HpaII CCGG 8 cut(s) 99, 464, 614, 882, 981, 1432, 1590, 1719
HphI GGTGA 8 cut(s) 777, 803, 817, 1101, 1186, 1575, 2062, 2140
Hpy166II GTNNAC 7 cut(s) 148, 427, 491, 498, 1036, 1063, 1288
Hpy188I TCNGA 7 cut(s) 53, 157, 744, 1122, 1340, 1716, 1809
Hpy8I GTNNAC 7 cut(s) 148, 427, 491, 498, 1036, 1063, 1288
Hpy99I CGWCG 4 cut(s) 40, 287, 536, 1699
HpyAV CCTTC 3 cut(s) 524, 1072, 1984
HpyCH4III ACNGT 5 cut(s) 495, 522, 621, 691, 1741
HpyCH4IV ACGT 5 cut(s) 9, 17, 240, 731, 1728
HpyCH4V TGCA 8 cut(s) 599, 658, 710, 845, 1130, 1242, 1406, 1892
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 371, 610, 1849
HpyF3I CTNAG 9 cut(s) 357, 366, 645, 1197, 1422, 1747, 1872, 2016, 2063
HpySE526I ACGT 5 cut(s) 9, 17, 240, 731, 1728
Hsp92I GRCGYC 3 cut(s) 9, 723, 1843
Hsp92II CATG 9 cut(s) 16, 392, 457, 824, 1096, 1507, 1672, 1693, 1757
HspAI GCGC 2 cut(s) 723, 1843
KasI GGCGCC 2 cut(s) 722, 1842
Kpn2I TCCGGA 1 cut(s) 463
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
LmnI GCTCC 3 cut(s) 935, 1348, 1417
Lsp1109I GCAGC 4 cut(s) 79, 694, 828, 1645
LweI GCATC 4 cut(s) 285, 586, 735, 832
MabI ACCWGGT 2 cut(s) 787, 2136
MaeI CTAG 3 cut(s) 1170, 1274, 1637
MaeII ACGT 5 cut(s) 9, 17, 240, 731, 1728
MaeIII GTNAC 4 cut(s) 805, 1107, 1581, 1741
MalI GATC 9 cut(s) 275, 405, 409, 447, 468, 1119, 1713, 1821, 1968
MbiI CCGCTC 1 cut(s) 1351
MboI GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
MflI RGATCY 1 cut(s) 1711
MhlI GDGCHC 2 cut(s) 478, 1516
MlsI TGGCCA 2 cut(s) 686, 1673
MluCI AATT 5 cut(s) 151, 183, 1376, 1398, 1443
MluNI TGGCCA 2 cut(s) 686, 1673
Mly113I GGCGCC 2 cut(s) 723, 1843
MlyI GAGTC 3 cut(s) 379, 1186, 1861
MmeI TCCRAC 2 cut(s) 141, 1739
Mox20I TGGCCA 2 cut(s) 686, 1673
MroI TCCGGA 1 cut(s) 463
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 3 cut(s) 513, 1223, 1769
MscI TGGCCA 2 cut(s) 686, 1673
MseI TTAA 5 cut(s) 348, 435, 903, 1281, 1626
MslI CAYNNNNRTG 1 cut(s) 581
Msp20I TGGCCA 2 cut(s) 686, 1673
MspA1I CMGCKG 1 cut(s) 1679
MspI CCGG 8 cut(s) 99, 464, 614, 882, 981, 1432, 1590, 1719
MspR9I CCNGG 9 cut(s) 504, 544, 683, 764, 789, 982, 1009, 1937, 2138
Mva1269I GAATGC 1 cut(s) 1406
MvaI CCWGG 8 cut(s) 504, 544, 683, 764, 789, 1009, 1937, 2138
MvnI CGCG 2 cut(s) 1466, 1971
MwoI GCNNNNNNNGC 4 cut(s) 41, 371, 610, 1849
NaeI GCCGGC 1 cut(s) 100
NarI GGCGCC 2 cut(s) 723, 1843
NciI CCSGG 1 cut(s) 982
NcoI CCATGG 1 cut(s) 1668
NdeII GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 9 cut(s) 16, 392, 457, 824, 1096, 1507, 1672, 1693, 1757
NmuCI GTSAC 4 cut(s) 805, 1107, 1581, 1741
NruI TCGCGA 1 cut(s) 1971
NspV TTCGAA 1 cut(s) 2160
OliI CACNNNNGTG 1 cut(s) 581
PagI TCATGA 2 cut(s) 12, 453
PcsI WCGNNNNNNNCGW 1 cut(s) 745
PctI GAATGC 1 cut(s) 1406
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 3 cut(s) 513, 1223, 1769
PfeI GAWTC 6 cut(s) 48, 54, 80, 1123, 1219, 1341
PflMI CCANNNNNTGG 1 cut(s) 1759
PinAI ACCGGT 1 cut(s) 1431
PkrI GCNGC 5 cut(s) 43, 69, 709, 818, 1635
PleI GAGTC 3 cut(s) 379, 1185, 1861
PluTI GGCGCC 2 cut(s) 726, 1846
PpsI GAGTC 3 cut(s) 379, 1185, 1861
Ppu21I YACGTR 1 cut(s) 1729
PshAI GACNNNNGTC 1 cut(s) 1748
Psp6I CCWGG 8 cut(s) 502, 542, 681, 762, 787, 1007, 1935, 2136
PspEI GGTNACC 2 cut(s) 1107, 1581
PspFI CCCAGC 1 cut(s) 950
PspGI CCWGG 8 cut(s) 502, 542, 681, 762, 787, 1007, 1935, 2136
PspOMI GGGCCC 1 cut(s) 474
PstI CTGCAG 1 cut(s) 712
PsuI RGATCY 1 cut(s) 1711
RruI TCGCGA 1 cut(s) 1971
RsaI GTAC 4 cut(s) 20, 1001, 1910, 2099
RsaNI GTAC 4 cut(s) 19, 1000, 1909, 2098
RseI CAYNNNNRTG 1 cut(s) 581
SalI GTCGAC 1 cut(s) 1286
SaqAI TTAA 5 cut(s) 348, 435, 903, 1281, 1626
SatI GCNGC 5 cut(s) 42, 68, 708, 817, 1634
Sau3AI GATC 9 cut(s) 273, 403, 407, 445, 466, 1117, 1711, 1819, 1966
ScaI AGTACT 1 cut(s) 1001
SchI GAGTC 3 cut(s) 379, 1186, 1861
ScrFI CCNGG 9 cut(s) 504, 544, 683, 764, 789, 982, 1009, 1937, 2138
SduI GDGCHC 2 cut(s) 478, 1516
SexAI ACCWGGT 2 cut(s) 787, 2136
SfaNI GCATC 4 cut(s) 285, 586, 735, 832
SfcI CTRYAG 3 cut(s) 256, 708, 1737
SfoI GGCGCC 2 cut(s) 724, 1844
SfuI TTCGAA 1 cut(s) 2160
SinI GGWCC 4 cut(s) 547, 663, 879, 1933
SmiMI CAYNNNNRTG 1 cut(s) 581
SmlI CTYRAG 3 cut(s) 341, 1550, 1954
SmoI CTYRAG 3 cut(s) 341, 1550, 1954
SnaBI TACGTA 1 cut(s) 1729
Sse9I AATT 5 cut(s) 151, 183, 1376, 1398, 1443
SsiI CCGC 7 cut(s) 5, 41, 88, 572, 1291, 1351, 1677
SspDI GGCGCC 2 cut(s) 722, 1842
SspMI CTAG 3 cut(s) 1170, 1274, 1637
StyD4I CCNGG 9 cut(s) 502, 542, 681, 762, 787, 980, 1007, 1935, 2136
StyI CCWWGG 4 cut(s) 324, 1478, 1668, 1847
TaaI ACNGT 5 cut(s) 495, 522, 621, 691, 1741
TaiI ACGT 5 cut(s) 12, 20, 243, 734, 1731
TasI AATT 5 cut(s) 151, 183, 1376, 1398, 1443
TatI WGTACW 2 cut(s) 999, 1908
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 6 cut(s) 48, 54, 80, 1123, 1219, 1341
Tru1I TTAA 5 cut(s) 348, 435, 903, 1281, 1626
Tru9I TTAA 5 cut(s) 348, 435, 903, 1281, 1626
TscAI CASTG 6 cut(s) 127, 527, 665, 1327, 1746, 2024
TseFI GTSAC 4 cut(s) 805, 1107, 1581, 1741
TseI GCWGC 4 cut(s) 67, 707, 816, 1633
Tsp45I GTSAC 4 cut(s) 805, 1107, 1581, 1741
TspDTI ATGAA 7 cut(s) 442, 683, 837, 1091, 1436, 1588, 1678
TspGWI ACGGA 4 cut(s) 593, 1058, 1712, 2085
TspRI CASTG 6 cut(s) 127, 527, 665, 1327, 1746, 2024
Van91I CCANNNNNTGG 1 cut(s) 1759
VpaK11BI GGWCC 4 cut(s) 547, 663, 879, 1933
XcmI CCANNNNNNNNNTGG 2 cut(s) 322, 550
XmiI GTMKAC 4 cut(s) 497, 1035, 1062, 1287
XmnI GAANNNNTTC 3 cut(s) 513, 1223, 1769
XspI CTAG 3 cut(s) 1170, 1274, 1637
ZraI GACGTC 1 cut(s) 10
ZrmI AGTACT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.