pycom04g21280

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011162
Physical Location & Seq
Forward (+)
22520063 .. 22522779
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21280.4

Sequence Viewer

Length: 1728 bp
ATGGCGGACATCATGACGTACAATGACATAGAGGCGACGGCGGCCCGAATCGGAATCGACCTCTCGCAGCTCGACCTCGATTCCATCCGCCTCCCTGCCGGCGAAGACTTTGGCATTATCAGTGATGATGAGGATATATACAATGTGGACAATTCGGAGTTGGACGCAGGGTTTAGCAACATAATTGTTGTTGATAACCTCCCTGTTGTTCCTTTGGAAAAGTTTGAGAAGCTCGAAAACGTCATTCGTAAAATCTATAGTCAGATTGGTGTGATCAAAGACGACGGCTTTTGGATGCCTCTGGACCCCGAAACCAATAAAACCTTGGGTTACTGCTTCCTTGAGTTTAATACTCCTCAGGAAGCTGAGCTTGCCAAAGAGAGGACTCATGGATACAAGCTGGATCGATCACATATTTTTGTTGTCAACATGTTTAACGACTTTGATCGGTTCATGAGTGTTCCGGATCAATGGGCCCCTCCAGAAAGTAAACCGTATACTCCGGGGGAAAACCTACAACAGTGGCTAACCGACGAAAAGGCCAGGGACCAGCTGGTCATTCGTTATGGTGCGGACACGGAAGTGCTATGGAACGATGCAAGGCACCTGAAGCCGGAACCGTTCTACAAACGCACTTTCTGGACTGAGAGCTTCGTGCAGTGGTCCCCTCTGGGGACTTACCTGGCCACTGTTCATAGGCAGGGTGCTGCAGTCTGGGGAGGCGCCTCCACGTTCAATCGTCTGATGCGTTATGCTCATTCCCAGGTTCGACTGATTGATTTCTCACCTGGTGAGAAATACTTGGTGACATACAGCAGCCATGAACCAAGCAATCCTCGTGATGCAAATAGGGTCGTGATAAACATTTTTGATGTGAGGACAGGAAAAGTAATGAGAGATTTTAAAGGAAGCCCAGATGATTTTGCTATTGGAGGAGCTGGAGGTGTTGCCGGGGTGTCTTGGCCTGTTTTCAAATGGGCTGGGGGAAAAGATGACAAGTACTTTGCCAGGATGGGTAAAAATGTTATTTCTGTATACGAGACGGAAACATTTTCTCTTGTAGACAAAAAGTCAATGAAGGTGGAAAATGTCATGGACTTCAGTTGGTCACCAACTGATCCGATTCTTGCACTATTTGTTCCAGAACTGGGTGGTGGCAACCAGCCTGCTAGGGTGAGTCTTGTTCAAATCCCTGCTAAGGAGGAGTTGAGGCAGAAGAATCTTTTCAGCGTCAGCGATTGCAAAATGTACTGGCAGAGCAATGGGGAGTATCTAGCTGTTAAGGTCGACCGCTACACCAAAACAAAGAAAAGTACATACACTGGATTTGAGCTTTTCAGAATCAAGGAGCGGGACATTCCTATCGAAGTCTTGGAATTGGAGAATAAGAATGACAAAATTATTGCATTCGCTTGGGAGCCTAAGGGCCACCGGTTCACAGTAATTCATTGTGATAACACAAACGCGAATAACAACCCAAGGCCTGACATAAGTTTCTACTCCATGCGAAGTGCTCACAATACTGGCCGTGTTTCAAAGCTCACTACTCTCAAGGGAAAGCAGGCAAATGCCCTTTACTGGTCACCTACCGGCCACTTCATTATTCTGGCAGGATTGAAAGGTTTTAATGGACAGCTAGAGTTTTACAATGTAGATGAGCTGGAAACCATGGCCACCGCTGAACATTTCATGGCGACAGATATCGAATGGGATCCGACTGGAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

576

Amino Acids

65.51

Weight (kDa)

5.3

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25780 AT5G27640 AT5G27640 AT5G27640
fragaria_vesca FvH4_6g00800
malus_domestica MD04G1241100.v1.1 MD12G1258100.v1.1
prunus_persica Prupe.6G358600_v2.0.a1
pyrus_communis pycom04g21280 pycom12g23670
rosa_chinensis RchiOBHm_Chr3g0448281
rosa_laevigata RLG00000025930
rosa_multiflora Rmu_sc0005582.1_g000005
rosa_roxburghii Rroxscaffold_6G00426430
rosa_rugosa Rorug02G0610000
rosa_samantha Rh3AG009600 Rh3BG009200 Rh3CG009200 Rh3DG009800
rosa_wichuraiana Rw3G000780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AccB1I GGYRCC 2 cut(s) 603, 722
AccBSI CCGCTC 1 cut(s) 1351
AccI GTMKAC 4 cut(s) 497, 1035, 1062, 1287
AccII CGCG 1 cut(s) 1466
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 7 cut(s) 5, 41, 88, 572, 1291, 1351, 1677
AclWI GGATC 5 cut(s) 411, 474, 1112, 1706, 1719
AcoI YGGCCR 4 cut(s) 684, 1525, 1591, 1671
AcuI CTGAAG 2 cut(s) 629, 1084
AcyI GRCGYC 1 cut(s) 723
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 4 cut(s) 20, 1001, 1250, 1315
AfiI CCNNNNNNNGG 6 cut(s) 381, 613, 672, 1148, 1198, 1578
AflIII ACRYGT 1 cut(s) 429
AgeI ACCGGT 1 cut(s) 1431
AgsI TTSAA 5 cut(s) 736, 973, 1187, 1536, 1618
AhdI GACNNNNNGTC 1 cut(s) 1069
AjnI CCWGG 5 cut(s) 542, 681, 762, 787, 1007
AleI CACNNNNGTG 1 cut(s) 581
Alw21I GWGCWC 1 cut(s) 1516
Alw26I GTCTC 1 cut(s) 1034
AlwI GGATC 5 cut(s) 411, 474, 1112, 1706, 1719
Aor13HI TCCGGA 1 cut(s) 463
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 3 cut(s) 67, 707, 816
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
AsiGI ACCGGT 1 cut(s) 1431
Asp700I GAANNNNTTC 1 cut(s) 1223
AspLEI GCGC 1 cut(s) 725
AspS9I GGNCC 7 cut(s) 43, 304, 474, 475, 547, 663, 1426
AsuC2I CCSGG 2 cut(s) 504, 952
AsuHPI GGTGA 6 cut(s) 777, 803, 817, 1101, 1186, 1575
AvaII GGWCC 3 cut(s) 304, 547, 663
AxyI CCTNAGG 2 cut(s) 357, 1422
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 2 cut(s) 686, 1673
BamHI GGATCC 1 cut(s) 1711
BanI GGYRCC 2 cut(s) 603, 722
BanII GRGCYC 1 cut(s) 478
BauI CACGAG 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 1516
BbvI GCAGC 3 cut(s) 79, 694, 828
BccI CCATC 2 cut(s) 92, 1006
BceAI ACGGC 3 cut(s) 54, 301, 1512
BciT130I CCWGG 5 cut(s) 544, 683, 764, 789, 1009
BciVI GTATCC 1 cut(s) 386
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 2 cut(s) 504, 952
BcoDI GTCTC 1 cut(s) 1034
BfaI CTAG 3 cut(s) 1170, 1274, 1637
BfmI CTRYAG 2 cut(s) 256, 708
BfoI RGCGCY 1 cut(s) 726
BfuI GTATCC 1 cut(s) 386
BisI GCNGC 4 cut(s) 42, 68, 708, 817
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 4 cut(s) 43, 69, 709, 818
BmcAI AGTACT 1 cut(s) 1001
Bme1390I CCNGG 7 cut(s) 504, 544, 683, 764, 789, 952, 1009
Bme18I GGWCC 3 cut(s) 304, 547, 663
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmgT120I GGNCC 7 cut(s) 43, 304, 474, 475, 547, 663, 1426
BmrFI CCNGG 7 cut(s) 504, 544, 683, 764, 789, 952, 1009
BmrI ACTGGG 1 cut(s) 1157
BmsI GCATC 4 cut(s) 285, 586, 735, 832
BmuI ACTGGG 1 cut(s) 1157
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 2 cut(s) 465, 960
Bpu10I CCTNAGC 1 cut(s) 1197
Bpu1102I GCTNAGC 1 cut(s) 366
BpuEI CTTGAG 2 cut(s) 362, 1535
BpuMI CCSGG 2 cut(s) 504, 952
Bsa29I ATCGAT 1 cut(s) 406
BsaHI GRCGYC 1 cut(s) 723
BsaJI CCNNGG 7 cut(s) 324, 503, 543, 762, 951, 1478, 1668
BsaWI WCCGGW 2 cut(s) 463, 1431
Bsc4I CCNNNNNNNGG 6 cut(s) 381, 613, 672, 1148, 1198, 1578
Bse118I RCCGGY 3 cut(s) 98, 1431, 1589
Bse1I ACTGG 6 cut(s) 1152, 1256, 1327, 1528, 1583, 1723
Bse21I CCTNAGG 2 cut(s) 357, 1422
Bse3DI GCAATG 1 cut(s) 1267
BseAI TCCGGA 1 cut(s) 463
BseBI CCWGG 5 cut(s) 544, 683, 764, 789, 1009
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 7 cut(s) 324, 503, 543, 762, 951, 1478, 1668
BseGI GGATG 3 cut(s) 84, 300, 1017
BseLI CCNNNNNNNGG 6 cut(s) 381, 613, 672, 1148, 1198, 1578
BseMI GCAATG 1 cut(s) 1267
BseMII CTCAG 3 cut(s) 357, 371, 636
BseNI ACTGG 6 cut(s) 1152, 1256, 1327, 1528, 1583, 1723
BseRI GAGGAG 3 cut(s) 345, 948, 1217
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 3 cut(s) 79, 694, 828
BseYI CCCAGC 1 cut(s) 980
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 1 cut(s) 1466
Bsh1285I CGRYCG 1 cut(s) 1291
BshNI GGYRCC 2 cut(s) 603, 722
BshTI ACCGGT 1 cut(s) 1431
BshVI ATCGAT 1 cut(s) 406
BsiEI CGRYCG 1 cut(s) 1291
BsiHKAI GWGCWC 1 cut(s) 1516
BsiSI CCGG 7 cut(s) 99, 464, 503, 614, 951, 1432, 1590
BslFI GGGAC 4 cut(s) 560, 649, 688, 1367
BslI CCNNNNNNNGG 6 cut(s) 381, 613, 672, 1148, 1198, 1578
BsmAI GTCTC 1 cut(s) 1034
BsmBI CGTCTC 1 cut(s) 1034
BsmFI GGGAC 4 cut(s) 560, 649, 688, 1367
BsmI GAATGC 1 cut(s) 1406
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 478, 1516
Bsp13I TCCGGA 1 cut(s) 463
Bsp143I GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
Bsp1720I GCTNAGC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 1668
BspACI CCGC 7 cut(s) 5, 41, 88, 572, 1291, 1351, 1677
BspCNI CTCAG 3 cut(s) 358, 370, 637
BspDI ATCGAT 1 cut(s) 406
BspEI TCCGGA 1 cut(s) 463
BspFNI CGCG 1 cut(s) 1466
BspHI TCATGA 2 cut(s) 12, 453
BspMAI CTGCAG 1 cut(s) 712
BspPI GGATC 5 cut(s) 411, 474, 1112, 1706, 1719
BspT107I GGYRCC 2 cut(s) 603, 722
BsrBI CCGCTC 1 cut(s) 1351
BsrDI GCAATG 1 cut(s) 1267
BsrFI RCCGGY 3 cut(s) 98, 1431, 1589
BsrI ACTGG 6 cut(s) 1152, 1256, 1327, 1528, 1583, 1723
BssAI RCCGGY 3 cut(s) 98, 1431, 1589
BssECI CCNNGG 7 cut(s) 324, 503, 543, 762, 951, 1478, 1668
BssMI GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
BssNAI GTATAC 2 cut(s) 498, 1036
BssNI GRCGYC 1 cut(s) 723
BssSI CACGAG 1 cut(s) 837
BssT1I CCWWGG 3 cut(s) 324, 1478, 1668
Bst1107I GTATAC 2 cut(s) 498, 1036
Bst2BI CACGAG 1 cut(s) 837
Bst2UI CCWGG 5 cut(s) 544, 683, 764, 789, 1009
Bst4CI ACNGT 5 cut(s) 495, 522, 621, 691, 1441
BstACI GRCGYC 1 cut(s) 723
BstC8I GCNNGC 4 cut(s) 100, 372, 1167, 1563
BstDEI CTNAG 5 cut(s) 357, 366, 645, 1197, 1422
BstDSI CCRYGG 1 cut(s) 1668
BstEII GGTNACC 2 cut(s) 1107, 1581
BstF5I GGATG 3 cut(s) 84, 300, 1017
BstFNI CGCG 1 cut(s) 1466
BstH2I RGCGCY 1 cut(s) 726
BstHHI GCGC 1 cut(s) 725
BstKTI GATC 7 cut(s) 276, 406, 410, 448, 469, 1120, 1714
BstMAI GTCTC 1 cut(s) 1034
BstMBI GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
BstMCI CGRYCG 1 cut(s) 1291
BstMWI GCNNNNNNNGC 3 cut(s) 41, 371, 610
BstNI CCWGG 5 cut(s) 544, 683, 764, 789, 1009
BstNSI RCATGY 1 cut(s) 433
BstPI GGTNACC 2 cut(s) 1107, 1581
BstSCI CCNGG 7 cut(s) 502, 542, 681, 762, 787, 950, 1007
BstSFI CTRYAG 2 cut(s) 256, 708
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 1 cut(s) 1466
BstV1I GCAGC 3 cut(s) 79, 694, 828
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 1711
BstYI RGATCY 1 cut(s) 1711
BstZ17I GTATAC 2 cut(s) 498, 1036
Bsu15I ATCGAT 1 cut(s) 406
Bsu36I CCTNAGG 2 cut(s) 357, 1422
BsuI GTATCC 1 cut(s) 386
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 1668
BtsCI GGATG 3 cut(s) 84, 300, 1017
BtsI GCAGTG 1 cut(s) 665
BtsIMutI CAGTG 5 cut(s) 127, 527, 665, 687, 1320
Cac8I GCNNGC 4 cut(s) 100, 372, 1167, 1563
CciI TCATGA 2 cut(s) 12, 453
CfoI GCGC 1 cut(s) 725
Cfr10I RCCGGY 3 cut(s) 98, 1431, 1589
Cfr13I GGNCC 7 cut(s) 43, 304, 474, 475, 547, 663, 1426
ClaI ATCGAT 1 cut(s) 406
CseI GACGC 2 cut(s) 173, 1219
CsiI ACCWGGT 1 cut(s) 787
Csp6I GTAC 4 cut(s) 19, 1000, 1249, 1314
CspAI ACCGGT 1 cut(s) 1431
CspCI CAANNNNNGTGG 2 cut(s) 1062, 1097
CviAII CATG 9 cut(s) 13, 389, 430, 454, 821, 1093, 1504, 1669, 1690
CviQI GTAC 4 cut(s) 19, 1000, 1249, 1314
DdeI CTNAG 5 cut(s) 357, 366, 645, 1197, 1422
DinI GGCGCC 1 cut(s) 724
DpnI GATC 7 cut(s) 275, 405, 409, 447, 468, 1119, 1713
DpnII GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
DraI TTTAAA 1 cut(s) 904
DraIII CACNNNGTG 1 cut(s) 791
DrdI GACNNNNNNGTC 1 cut(s) 554
DriI GACNNNNNGTC 1 cut(s) 1069
DseDI GACNNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 4 cut(s) 684, 1525, 1591, 1671
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EciI GGCGGA 2 cut(s) 20, 77
Eco130I CCWWGG 3 cut(s) 324, 1478, 1668
Eco147I AGGCCT 1 cut(s) 1483
Eco24I GRGCYC 1 cut(s) 478
Eco32I GATATC 1 cut(s) 1702
Eco47I GGWCC 3 cut(s) 304, 547, 663
Eco57I CTGAAG 2 cut(s) 629, 1084
Eco81I CCTNAGG 2 cut(s) 357, 1422
Eco91I GGTNACC 2 cut(s) 1107, 1581
EcoO109I RGGNCCY 1 cut(s) 475
EcoO65I GGTNACC 2 cut(s) 1107, 1581
EcoRII CCWGG 5 cut(s) 542, 681, 762, 787, 1007
EcoRV GATATC 1 cut(s) 1702
EcoT14I CCWWGG 3 cut(s) 324, 1478, 1668
EcoT38I GRGCYC 1 cut(s) 478
EgeI GGCGCC 1 cut(s) 724
EheI GGCGCC 1 cut(s) 724
ErhI CCWWGG 3 cut(s) 324, 1478, 1668
Esp3I CGTCTC 1 cut(s) 1034
FaeI CATG 9 cut(s) 16, 392, 433, 457, 824, 1096, 1507, 1672, 1693
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 4 cut(s) 560, 649, 688, 1367
FatI CATG 9 cut(s) 12, 388, 429, 453, 820, 1092, 1503, 1668, 1689
FauI CCCGC 1 cut(s) 1344
FbaI TGATCA 1 cut(s) 273
FblI GTMKAC 4 cut(s) 497, 1035, 1062, 1287
Fnu4HI GCNGC 4 cut(s) 42, 68, 708, 817
FokI GGATG 3 cut(s) 71, 307, 1024
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 4 cut(s) 42, 68, 708, 817
FspBI CTAG 3 cut(s) 1170, 1274, 1637
GlaI GCGC 1 cut(s) 724
GluI GCNGC 4 cut(s) 42, 68, 708, 817
GsaI CCCAGC 1 cut(s) 984
GsuI CTGGAG 2 cut(s) 465, 960
HaeII RGCGCY 1 cut(s) 726
HapII CCGG 7 cut(s) 99, 464, 503, 614, 951, 1432, 1590
HgaI GACGC 2 cut(s) 173, 1219
HhaI GCGC 1 cut(s) 725
Hin1I GRCGYC 1 cut(s) 723
Hin1II CATG 9 cut(s) 16, 392, 433, 457, 824, 1096, 1507, 1672, 1693
Hin6I GCGC 1 cut(s) 723
HinP1I GCGC 1 cut(s) 723
HincII GTYRAC 2 cut(s) 427, 1288
HindII GTYRAC 2 cut(s) 427, 1288
HinfI GANTC 8 cut(s) 48, 54, 80, 385, 1123, 1177, 1219, 1341
HpaII CCGG 7 cut(s) 99, 464, 503, 614, 951, 1432, 1590
HphI GGTGA 6 cut(s) 777, 803, 817, 1101, 1186, 1575
Hpy166II GTNNAC 8 cut(s) 148, 427, 491, 498, 1036, 1063, 1288, 1437
Hpy188I TCNGA 7 cut(s) 53, 157, 264, 744, 1122, 1340, 1716
Hpy8I GTNNAC 8 cut(s) 148, 427, 491, 498, 1036, 1063, 1288, 1437
Hpy99I CGWCG 3 cut(s) 40, 287, 536
HpyAV CCTTC 2 cut(s) 1072, 1716
HpyCH4III ACNGT 5 cut(s) 495, 522, 621, 691, 1441
HpyCH4IV ACGT 3 cut(s) 17, 240, 731
HpyCH4V TGCA 7 cut(s) 599, 658, 710, 845, 1130, 1242, 1406
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 371, 610
HpyF3I CTNAG 5 cut(s) 357, 366, 645, 1197, 1422
HpySE526I ACGT 3 cut(s) 17, 240, 731
Hsp92I GRCGYC 1 cut(s) 723
Hsp92II CATG 9 cut(s) 16, 392, 433, 457, 824, 1096, 1507, 1672, 1693
HspAI GCGC 1 cut(s) 723
KasI GGCGCC 1 cut(s) 722
Kpn2I TCCGGA 1 cut(s) 463
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
LmnI GCTCC 3 cut(s) 935, 1348, 1417
Lsp1109I GCAGC 3 cut(s) 79, 694, 828
LweI GCATC 4 cut(s) 285, 586, 735, 832
MabI ACCWGGT 1 cut(s) 787
MaeI CTAG 3 cut(s) 1170, 1274, 1637
MaeII ACGT 3 cut(s) 17, 240, 731
MaeIII GTNAC 4 cut(s) 329, 805, 1107, 1581
MalI GATC 7 cut(s) 275, 405, 409, 447, 468, 1119, 1713
MbiI CCGCTC 1 cut(s) 1351
MboI GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
MboII GAAGA 2 cut(s) 116, 1228
MflI RGATCY 1 cut(s) 1711
MhlI GDGCHC 2 cut(s) 478, 1516
MlsI TGGCCA 2 cut(s) 686, 1673
MluCI AATT 5 cut(s) 151, 183, 1376, 1398, 1443
MluNI TGGCCA 2 cut(s) 686, 1673
Mly113I GGCGCC 1 cut(s) 723
MlyI GAGTC 2 cut(s) 379, 1186
MmeI TCCRAC 1 cut(s) 141
Mox20I TGGCCA 2 cut(s) 686, 1673
MroI TCCGGA 1 cut(s) 463
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 1223
MscI TGGCCA 2 cut(s) 686, 1673
MseI TTAA 5 cut(s) 348, 435, 903, 1281, 1626
MslI CAYNNNNRTG 1 cut(s) 581
Msp20I TGGCCA 2 cut(s) 686, 1673
MspA1I CMGCKG 2 cut(s) 553, 1679
MspI CCGG 7 cut(s) 99, 464, 503, 614, 951, 1432, 1590
MspR9I CCNGG 7 cut(s) 504, 544, 683, 764, 789, 952, 1009
Mva1269I GAATGC 1 cut(s) 1406
MvaI CCWGG 5 cut(s) 544, 683, 764, 789, 1009
MvnI CGCG 1 cut(s) 1466
MwoI GCNNNNNNNGC 3 cut(s) 41, 371, 610
NaeI GCCGGC 1 cut(s) 100
NarI GGCGCC 1 cut(s) 723
NciI CCSGG 2 cut(s) 504, 952
NcoI CCATGG 1 cut(s) 1668
NdeII GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 9 cut(s) 16, 392, 433, 457, 824, 1096, 1507, 1672, 1693
NmuCI GTSAC 3 cut(s) 805, 1107, 1581
NspI RCATGY 1 cut(s) 433
OliI CACNNNNGTG 1 cut(s) 581
PagI TCATGA 2 cut(s) 12, 453
PceI AGGCCT 1 cut(s) 1483
PciI ACATGT 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 745
PctI GAATGC 1 cut(s) 1406
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 1223
PfeI GAWTC 6 cut(s) 48, 54, 80, 1123, 1219, 1341
PinAI ACCGGT 1 cut(s) 1431
PkrI GCNGC 4 cut(s) 43, 69, 709, 818
PleI GAGTC 2 cut(s) 379, 1185
PluTI GGCGCC 1 cut(s) 726
PpsI GAGTC 2 cut(s) 379, 1185
PscI ACATGT 1 cut(s) 429
Psp6I CCWGG 5 cut(s) 542, 681, 762, 787, 1007
PspEI GGTNACC 2 cut(s) 1107, 1581
PspFI CCCAGC 1 cut(s) 980
PspGI CCWGG 5 cut(s) 542, 681, 762, 787, 1007
PspOMI GGGCCC 1 cut(s) 474
PspPI GGNCC 7 cut(s) 43, 304, 474, 475, 547, 663, 1426
PstI CTGCAG 1 cut(s) 712
PsuI RGATCY 1 cut(s) 1711
PvuII CAGCTG 1 cut(s) 553
RsaI GTAC 4 cut(s) 20, 1001, 1250, 1315
RsaNI GTAC 4 cut(s) 19, 1000, 1249, 1314
RseI CAYNNNNRTG 1 cut(s) 581
SalI GTCGAC 1 cut(s) 1286
SaqAI TTAA 5 cut(s) 348, 435, 903, 1281, 1626
SatI GCNGC 4 cut(s) 42, 68, 708, 817
Sau3AI GATC 7 cut(s) 273, 403, 407, 445, 466, 1117, 1711
Sau96I GGNCC 7 cut(s) 43, 304, 474, 475, 547, 663, 1426
ScaI AGTACT 1 cut(s) 1001
SchI GAGTC 2 cut(s) 379, 1186
ScrFI CCNGG 7 cut(s) 504, 544, 683, 764, 789, 952, 1009
SduI GDGCHC 2 cut(s) 478, 1516
SexAI ACCWGGT 1 cut(s) 787
SfaNI GCATC 4 cut(s) 285, 586, 735, 832
SfcI CTRYAG 2 cut(s) 256, 708
SfoI GGCGCC 1 cut(s) 724
SinI GGWCC 3 cut(s) 304, 547, 663
SmiMI CAYNNNNRTG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 341, 1550
SmoI CTYRAG 2 cut(s) 341, 1550
Sse9I AATT 5 cut(s) 151, 183, 1376, 1398, 1443
SseBI AGGCCT 1 cut(s) 1483
SsiI CCGC 7 cut(s) 5, 41, 88, 572, 1291, 1351, 1677
SspDI GGCGCC 1 cut(s) 722
SspMI CTAG 3 cut(s) 1170, 1274, 1637
StuI AGGCCT 1 cut(s) 1483
StyD4I CCNGG 7 cut(s) 502, 542, 681, 762, 787, 950, 1007
StyI CCWWGG 3 cut(s) 324, 1478, 1668
TaaI ACNGT 5 cut(s) 495, 522, 621, 691, 1441
TaiI ACGT 3 cut(s) 20, 243, 734
TaqI TCGA 9 cut(s) 57, 72, 78, 234, 406, 769, 1287, 1365, 1704
TasI AATT 5 cut(s) 151, 183, 1376, 1398, 1443
TatI WGTACW 3 cut(s) 999, 1248, 1313
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 6 cut(s) 48, 54, 80, 1123, 1219, 1341
Tru1I TTAA 5 cut(s) 348, 435, 903, 1281, 1626
Tru9I TTAA 5 cut(s) 348, 435, 903, 1281, 1626
TscAI CASTG 5 cut(s) 127, 527, 665, 694, 1327
TseFI GTSAC 3 cut(s) 805, 1107, 1581
TseI GCWGC 3 cut(s) 67, 707, 816
Tsp45I GTSAC 3 cut(s) 805, 1107, 1581
TspDTI ATGAA 7 cut(s) 442, 683, 837, 1091, 1436, 1588, 1678
TspGWI ACGGA 2 cut(s) 593, 1058
TspRI CASTG 5 cut(s) 127, 527, 665, 694, 1327
VpaK11BI GGWCC 3 cut(s) 304, 547, 663
XceI RCATGY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 2 cut(s) 322, 550
XmiI GTMKAC 4 cut(s) 497, 1035, 1062, 1287
XmnI GAANNNNTTC 1 cut(s) 1223
XspI CTAG 3 cut(s) 1170, 1274, 1637
ZrmI AGTACT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.