MD12G1258100.v1.1

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011162
Physical Location & Seq
Forward (+)
32505156 .. 32508915
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1258100.v1.1.491

Sequence Viewer

Length: 2169 bp
ATGGCGGACGTCATGGCGTACAACGACATAGAGGCGACGGCGGCCCGAATCGGAATTGACCTCTCGCAGCTCGACCTCGATTCCATCCGCCTCCCTCCCGGCGAAGACTTTGGCATTATCAGTGACGATGAGGATGTATACAAAGTGGACAATTCGGAGTTGGACTCAGGGTTTGGCAGCATAATTGTTGTTGATAACCTCCCTGTTGTTCCTTTGGAAAAGTTTGAGAAACTCGAAAACGTCATTCGTAAAATCTACAGTCAGATTGGTGTGATCAAAGACGATGGCTTTTGGATGCCTCTTGACCCCGAAACCAATAAAACCTTGGGTTATTGCTTCCTTGAGTTTAATACTCCTCAGGAAGCTGAGCTTGCCAAAGAGAGGACTCATGGATACAAGCTGGATCGATCACATATTTTTGTTGTGAACATGTTTGACGACTTTGATCGGTTCATGAAAGTTCCGGATCAATGGGCCCCTCCTGAAAGTAAACCGTATACTCCAGGGGAAAACCTTCAACAGTGGCTGACTGACGAAAAGGCCAGGGACCAAATGGTCATTCGTTATGGTGCGGACACAGAAGTGCTATGGAATGATGCAAGGCACCTGAAGCCGGAACCGTTCTACAAACGCACTTTCTGGACTGAGAGCTTCGTGCAGTGGTCCCCTCTAGGGACTTACCTGGCCACTGTTCACAGACTGGGTGTTGCAGTCTGGGGAGGCGCCTCTACGTTCAATCGTCTGATGCGTTATGCTCATTCCCAGGTTCGACTGATTGATTTCTCACCTGGTGAGAAATACTTGGTGACTTACAGCAGCCATGAACCAAGCAATCCTCGTGATGCAAATAGGGTAGTGATAAACATTTTTGATGTGAGGACCGGAAAAGTAATGAGAGATTTTAAAGGAAGCCCAGATGATTTTGCTATTGGAGGAGCTGGAGGTGTTGCCGGGGTGTCTTGGCCTGTTTTCAAATGGGCCGGGGGAAAAGATGACAAGTACTTTGCCAGAATGGGAAAAAACGTCATCTCTGTATATGAAACGGAGACATTTTCCCTTGTAGACAAAAAGTCAATGAAGGTGGAAAATGTTATGGACTTCAGTTGGTCACCAACTGATCCAATTCTTGCGCTATTTGTTCCTGAACTGGGTGGTGGCAACCAGCCTGCGAGGGTGAGTCTTGTTCAAATCCCCTCTAAGGAGGAGTTGCGGCAGAAGAATCTTTTCAGCGTCAGCGATTGCAAAATGTACTGGCAGAGCAACGGGGAGTATCTAGCTGTTAAAGTTGATCGCTACACCAAAACAAAGAAAAGCACATACACTGGATTTGAGCTTTTCAGAATCAAGGAGCGGGACATTCCTATTGAAGTCTTGGAATTGGAGAATAAAAATGACAAAATTATTGCATTCGCTTGGGAGCCTAAGGGCCACAGGTTCGCAGTAATTCATTGTGATAACACGAACGCGAATAACAACCCAAGGCCCGACATAAGTTTCTACTCCATGCGAAGTGCTCACAATACTGGCCGTGTTTCGAAGCTCACTACTCTCAAAGGAAAGCAGGCAAATGCTCTCTACTGGTCACCTACCGGGCGCTTCATCATTTTGGCAGGATTGAAAGGTTTTAATGGGCAGCTAGAGTTTTACAATGTCGATGAGCTGGAAACCATGGCCACCGCTGAACATTTCATGGCGACAGATATTGAATGGGATCCGACTGGAAGATACGTAGCTACTGCAGTGACCTCAGTCCATGAGATGGAGAACGGCTTCAACATCTGGACATTCAATGGCAAGCTACTTTATCGGACACTAAAGGATCATTTCTTTCAGTTCTTATGGCGCCCAAGGCCACCATCATTGCTGACTCCTGAGAAAGAGGAGGAGATTGCAAAGAACTTGAAGAAGTACAGCAAGAAGTACGAGGCTGAGGACCAAGATGTTTCAATCTTCTTGAGCGAGCAGGATCGTGAGAAGAGAAGAATGTTGAAGGAGGAATGGGAGAAGTGGGTGGCTCACTGGAAGCAGAGGCACGAAGAAGAGAAAGAGGCAAGGAAAAATCTTAGGGACGGAGAAGCAAGTGATGAGGAAGAGGAGTACGAGGCTAAAGCTATTGAGGTCGAGGAGGTGATAAACCAGGTAGAGGAGGTCGTCAGCTTCGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

723

Amino Acids

83.34

Weight (kDa)

5.19

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 79 - 136 2.3e-06 RNA recognition motif
eIF2A PF08662 406 - 612 5.9e-59 Eukaryotic translation initiation factor eIF2A
Beta-prop_THOC3 PF25174 464 - 596 8.2e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25780 AT5G27640 AT5G27640 AT5G27640
fragaria_vesca FvH4_6g00800
malus_domestica MD04G1241100.v1.1 MD12G1258100.v1.1
prunus_persica Prupe.6G358600_v2.0.a1
pyrus_communis pycom04g21280 pycom12g23670
rosa_chinensis RchiOBHm_Chr3g0448281
rosa_laevigata RLG00000025930
rosa_multiflora Rmu_sc0005582.1_g000005
rosa_roxburghii Rroxscaffold_6G00426430
rosa_rugosa Rorug02G0610000
rosa_samantha Rh3AG009600 Rh3BG009200 Rh3CG009200 Rh3DG009800
rosa_wichuraiana Rw3G000780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AatII GACGTC 1 cut(s) 12
AccB1I GGYRCC 3 cut(s) 603, 722, 1842
AccB7I CCANNNNNTGG 1 cut(s) 1759
AccBSI CCGCTC 1 cut(s) 1351
AccI GTMKAC 3 cut(s) 138, 497, 1062
AccII CGCG 1 cut(s) 1466
AccIII TCCGGA 1 cut(s) 463
AciI CCGC 7 cut(s) 5, 41, 88, 572, 1210, 1351, 1677
AclWI GGATC 7 cut(s) 411, 474, 1112, 1706, 1719, 1827, 1974
AcoI YGGCCR 3 cut(s) 684, 1525, 1671
AcuI CTGAAG 2 cut(s) 629, 1084
AcyI GRCGYC 3 cut(s) 9, 723, 1843
AdeI CACNNNGTG 1 cut(s) 791
AfaI GTAC 6 cut(s) 20, 1001, 1250, 1910, 1922, 2099
AfiI CCNNNNNNNGG 7 cut(s) 381, 613, 672, 1148, 1198, 1759, 2143
AflIII ACRYGT 1 cut(s) 429
AhdI GACNNNNNGTC 1 cut(s) 1069
AjnI CCWGG 6 cut(s) 502, 542, 681, 762, 787, 2136
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AleI CACNNNNGTG 1 cut(s) 581
Alw21I GWGCWC 1 cut(s) 1516
Alw26I GTCTC 1 cut(s) 1040
AlwI GGATC 7 cut(s) 411, 474, 1112, 1706, 1719, 1827, 1974
AlwNI CAGNNNCTG 1 cut(s) 526
Aor13HI TCCGGA 1 cut(s) 463
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 4 cut(s) 67, 177, 816, 1633
ArsI GACNNNNNNTTYG 4 cut(s) 155, 187, 986, 1018
Asp700I GAANNNNTTC 3 cut(s) 513, 1223, 1769
AspLEI GCGC 4 cut(s) 725, 1132, 1596, 1845
AsuC2I CCSGG 4 cut(s) 99, 952, 982, 1591
AsuHPI GGTGA 7 cut(s) 777, 803, 817, 1101, 1186, 1575, 2140
AsuII TTCGAA 2 cut(s) 1535, 2160
AvaII GGWCC 4 cut(s) 547, 663, 879, 1933
AxyI CCTNAGG 2 cut(s) 357, 1422
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 2 cut(s) 686, 1673
BamHI GGATCC 1 cut(s) 1711
BanI GGYRCC 3 cut(s) 603, 722, 1842
BanII GRGCYC 1 cut(s) 478
BauI CACGAG 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 1516
BbvCI CCTCAGC 1 cut(s) 1929
BbvI GCAGC 4 cut(s) 79, 189, 828, 1645
BccI CCATC 4 cut(s) 92, 278, 1753, 1864
BceAI ACGGC 3 cut(s) 54, 1512, 1783
BciT130I CCWGG 6 cut(s) 504, 544, 683, 764, 789, 2138
BciVI GTATCC 1 cut(s) 386
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 4 cut(s) 99, 952, 982, 1591
BcoDI GTCTC 1 cut(s) 1040
BfaI CTAG 3 cut(s) 671, 1274, 1637
BfmI CTRYAG 2 cut(s) 256, 1737
BfoI RGCGCY 3 cut(s) 726, 1597, 1846
BfuI GTATCC 1 cut(s) 386
BisI GCNGC 6 cut(s) 42, 68, 178, 817, 1211, 1634
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 6 cut(s) 43, 69, 179, 818, 1212, 1635
BmcAI AGTACT 1 cut(s) 1001
Bme18I GGWCC 4 cut(s) 547, 663, 879, 1933
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmrI ACTGGG 2 cut(s) 710, 1157
BmsI GCATC 4 cut(s) 285, 586, 735, 832
BmuI ACTGGG 2 cut(s) 710, 1157
BoxI GACNNNNGTC 1 cut(s) 1748
BpiI GAAGAC 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 2 cut(s) 486, 960
Bpu10I CCTNAGC 1 cut(s) 1929
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 2 cut(s) 1535, 2160
BpuEI CTTGAG 2 cut(s) 362, 1975
BpuMI CCSGG 4 cut(s) 99, 952, 982, 1591
Bsa29I ATCGAT 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 1729
BsaHI GRCGYC 3 cut(s) 9, 723, 1843
BsaJI CCNNGG 9 cut(s) 324, 503, 543, 762, 951, 981, 1478, 1668, 1847
BsaWI WCCGGW 2 cut(s) 463, 881
Bsc4I CCNNNNNNNGG 7 cut(s) 381, 613, 672, 1148, 1198, 1759, 2143
Bse1I ACTGG 8 cut(s) 705, 1152, 1256, 1327, 1528, 1583, 1723, 2024
Bse21I CCTNAGG 2 cut(s) 357, 1422
Bse3DI GCAATG 1 cut(s) 1859
BseAI TCCGGA 1 cut(s) 463
BseBI CCWGG 6 cut(s) 504, 544, 683, 764, 789, 2138
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 9 cut(s) 324, 503, 543, 762, 951, 981, 1478, 1668, 1847
BseGI GGATG 3 cut(s) 84, 139, 300
BseLI CCNNNNNNNGG 7 cut(s) 381, 613, 672, 1148, 1198, 1759, 2143
BseMI GCAATG 1 cut(s) 1859
BseMII CTCAG 7 cut(s) 180, 357, 371, 636, 1761, 1863, 1920
BseNI ACTGG 8 cut(s) 705, 1152, 1256, 1327, 1528, 1583, 1723, 2024
BseRI GAGGAG 8 cut(s) 345, 948, 1217, 1895, 1898, 2108, 2138, 2159
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 4 cut(s) 79, 189, 828, 1645
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 1 cut(s) 1466
BshNI GGYRCC 3 cut(s) 603, 722, 1842
BshVI ATCGAT 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 1516
BsiSI CCGG 7 cut(s) 99, 464, 614, 882, 951, 981, 1590
BslFI GGGAC 5 cut(s) 560, 649, 688, 1367, 2081
BslI CCNNNNNNNGG 7 cut(s) 381, 613, 672, 1148, 1198, 1759, 2143
BsmAI GTCTC 1 cut(s) 1040
BsmFI GGGAC 5 cut(s) 560, 649, 688, 1367, 2081
BsmI GAATGC 1 cut(s) 1406
Bsp119I TTCGAA 2 cut(s) 1535, 2160
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 478, 1516
Bsp13I TCCGGA 1 cut(s) 463
Bsp1720I GCTNAGC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 1668
BspACI CCGC 7 cut(s) 5, 41, 88, 572, 1210, 1351, 1677
BspCNI CTCAG 7 cut(s) 179, 358, 370, 637, 1760, 1864, 1921
BspDI ATCGAT 1 cut(s) 406
BspEI TCCGGA 1 cut(s) 463
BspFNI CGCG 1 cut(s) 1466
BspHI TCATGA 1 cut(s) 453
BspMAI CTGCAG 1 cut(s) 1741
BspPI GGATC 7 cut(s) 411, 474, 1112, 1706, 1719, 1827, 1974
BspT104I TTCGAA 2 cut(s) 1535, 2160
BspT107I GGYRCC 3 cut(s) 603, 722, 1842
BsrBI CCGCTC 1 cut(s) 1351
BsrDI GCAATG 1 cut(s) 1859
BsrI ACTGG 8 cut(s) 705, 1152, 1256, 1327, 1528, 1583, 1723, 2024
BssECI CCNNGG 9 cut(s) 324, 503, 543, 762, 951, 981, 1478, 1668, 1847
BssNAI GTATAC 2 cut(s) 139, 498
BssNI GRCGYC 3 cut(s) 9, 723, 1843
BssSI CACGAG 1 cut(s) 837
BssT1I CCWWGG 4 cut(s) 324, 1478, 1668, 1847
Bst1107I GTATAC 2 cut(s) 139, 498
Bst2BI CACGAG 1 cut(s) 837
Bst2UI CCWGG 6 cut(s) 504, 544, 683, 764, 789, 2138
Bst4CI ACNGT 5 cut(s) 260, 495, 522, 621, 691
Bst6I CTCTTC 3 cut(s) 1970, 2034, 2085
BstACI GRCGYC 3 cut(s) 9, 723, 1843
BstBAI YACGTR 1 cut(s) 1729
BstBI TTCGAA 2 cut(s) 1535, 2160
BstC8I GCNNGC 5 cut(s) 372, 1167, 1563, 1796, 1961
BstDSI CCRYGG 1 cut(s) 1668
BstEII GGTNACC 2 cut(s) 1107, 1581
BstF5I GGATG 3 cut(s) 84, 139, 300
BstFNI CGCG 1 cut(s) 1466
BstH2I RGCGCY 3 cut(s) 726, 1597, 1846
BstHHI GCGC 4 cut(s) 725, 1132, 1596, 1845
BstMAI GTCTC 1 cut(s) 1040
BstMWI GCNNNNNNNGC 4 cut(s) 41, 371, 610, 1849
BstNI CCWGG 6 cut(s) 504, 544, 683, 764, 789, 2138
BstNSI RCATGY 1 cut(s) 433
BstPAI GACNNNNGTC 1 cut(s) 1748
BstPI GGTNACC 2 cut(s) 1107, 1581
BstSFI CTRYAG 2 cut(s) 256, 1737
BstSLI GKGCMC 1 cut(s) 478
BstSNI TACGTA 1 cut(s) 1729
BstUI CGCG 1 cut(s) 1466
BstV1I GCAGC 4 cut(s) 79, 189, 828, 1645
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 1711
BstYI RGATCY 1 cut(s) 1711
BstZ17I GTATAC 2 cut(s) 139, 498
Bsu15I ATCGAT 1 cut(s) 406
Bsu36I CCTNAGG 2 cut(s) 357, 1422
BsuI GTATCC 1 cut(s) 386
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 1668
BtsCI GGATG 3 cut(s) 84, 139, 300
BtsI GCAGTG 2 cut(s) 665, 1746
BtsIMutI CAGTG 7 cut(s) 127, 527, 665, 687, 1320, 1746, 2017
Cac8I GCNNGC 5 cut(s) 372, 1167, 1563, 1796, 1961
CaiI CAGNNNCTG 1 cut(s) 526
CciI TCATGA 1 cut(s) 453
CfoI GCGC 4 cut(s) 725, 1132, 1596, 1845
ClaI ATCGAT 1 cut(s) 406
CseI GACGC 1 cut(s) 1219
CsiI ACCWGGT 2 cut(s) 787, 2136
Csp6I GTAC 6 cut(s) 19, 1000, 1249, 1909, 1921, 2098
CspCI CAANNNNNGTGG 4 cut(s) 1062, 1097, 1842, 1877
CviAII CATG 9 cut(s) 13, 389, 430, 454, 821, 1504, 1669, 1690, 1754
CviQI GTAC 6 cut(s) 19, 1000, 1249, 1909, 1921, 2098
DinI GGCGCC 2 cut(s) 724, 1844
DraI TTTAAA 1 cut(s) 904
DraIII CACNNNGTG 1 cut(s) 791
DrdI GACNNNNNNGTC 1 cut(s) 554
DriI GACNNNNNGTC 1 cut(s) 1069
DseDI GACNNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 3 cut(s) 684, 1525, 1671
Eam1104I CTCTTC 3 cut(s) 1970, 2034, 2085
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 3 cut(s) 1970, 2034, 2085
EciI GGCGGA 2 cut(s) 20, 77
Eco105I TACGTA 1 cut(s) 1729
Eco130I CCWWGG 4 cut(s) 324, 1478, 1668, 1847
Eco24I GRGCYC 1 cut(s) 478
Eco47I GGWCC 4 cut(s) 547, 663, 879, 1933
Eco57I CTGAAG 2 cut(s) 629, 1084
Eco81I CCTNAGG 2 cut(s) 357, 1422
Eco91I GGTNACC 2 cut(s) 1107, 1581
EcoO109I RGGNCCY 1 cut(s) 475
EcoO65I GGTNACC 2 cut(s) 1107, 1581
EcoRII CCWGG 6 cut(s) 502, 542, 681, 762, 787, 2136
EcoT14I CCWWGG 4 cut(s) 324, 1478, 1668, 1847
EcoT38I GRGCYC 1 cut(s) 478
EgeI GGCGCC 2 cut(s) 724, 1844
EheI GGCGCC 2 cut(s) 724, 1844
ErhI CCWWGG 4 cut(s) 324, 1478, 1668, 1847
FaeI CATG 9 cut(s) 16, 392, 433, 457, 824, 1507, 1672, 1693, 1757
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 5 cut(s) 560, 649, 688, 1367, 2081
FatI CATG 9 cut(s) 12, 388, 429, 453, 820, 1503, 1668, 1689, 1753
FauI CCCGC 1 cut(s) 1344
FbaI TGATCA 1 cut(s) 273
FblI GTMKAC 3 cut(s) 138, 497, 1062
Fnu4HI GCNGC 6 cut(s) 42, 68, 178, 817, 1211, 1634
FokI GGATG 3 cut(s) 71, 146, 307
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 6 cut(s) 42, 68, 178, 817, 1211, 1634
FspBI CTAG 3 cut(s) 671, 1274, 1637
GlaI GCGC 4 cut(s) 724, 1131, 1595, 1844
GluI GCNGC 6 cut(s) 42, 68, 178, 817, 1211, 1634
GsuI CTGGAG 2 cut(s) 486, 960
HaeII RGCGCY 3 cut(s) 726, 1597, 1846
HapII CCGG 7 cut(s) 99, 464, 614, 882, 951, 981, 1590
HgaI GACGC 1 cut(s) 1219
HhaI GCGC 4 cut(s) 725, 1132, 1596, 1845
Hin1I GRCGYC 3 cut(s) 9, 723, 1843
Hin1II CATG 9 cut(s) 16, 392, 433, 457, 824, 1507, 1672, 1693, 1757
Hin6I GCGC 4 cut(s) 723, 1130, 1594, 1843
HinP1I GCGC 4 cut(s) 723, 1130, 1594, 1843
HinfI GANTC 8 cut(s) 48, 80, 164, 385, 1177, 1219, 1341, 1867
HpaII CCGG 7 cut(s) 99, 464, 614, 882, 951, 981, 1590
HphI GGTGA 7 cut(s) 777, 803, 817, 1101, 1186, 1575, 2140
Hpy166II GTNNAC 7 cut(s) 139, 148, 427, 491, 498, 694, 1063
Hpy188I TCNGA 7 cut(s) 53, 157, 264, 744, 1340, 1716, 1809
Hpy8I GTNNAC 7 cut(s) 139, 148, 427, 491, 498, 694, 1063
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 524, 1072, 1984
HpyCH4III ACNGT 5 cut(s) 260, 495, 522, 621, 691
HpyCH4IV ACGT 5 cut(s) 9, 240, 731, 1023, 1728
HpyCH4V TGCA 8 cut(s) 599, 658, 710, 845, 1242, 1406, 1739, 1892
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 371, 610, 1849
HpySE526I ACGT 5 cut(s) 9, 240, 731, 1023, 1728
Hsp92I GRCGYC 3 cut(s) 9, 723, 1843
Hsp92II CATG 9 cut(s) 16, 392, 433, 457, 824, 1507, 1672, 1693, 1757
HspAI GCGC 4 cut(s) 723, 1130, 1594, 1843
KasI GGCGCC 2 cut(s) 722, 1842
Kpn2I TCCGGA 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 273
LmnI GCTCC 3 cut(s) 935, 1348, 1417
Lsp1109I GCAGC 4 cut(s) 79, 189, 828, 1645
LweI GCATC 4 cut(s) 285, 586, 735, 832
MabI ACCWGGT 2 cut(s) 787, 2136
MaeI CTAG 3 cut(s) 671, 1274, 1637
MaeII ACGT 5 cut(s) 9, 240, 731, 1023, 1728
MaeIII GTNAC 5 cut(s) 122, 805, 1107, 1581, 1741
MbiI CCGCTC 1 cut(s) 1351
MflI RGATCY 1 cut(s) 1711
MhlI GDGCHC 2 cut(s) 478, 1516
MlsI TGGCCA 2 cut(s) 686, 1673
MluCI AATT 7 cut(s) 54, 151, 183, 1122, 1376, 1398, 1443
MluNI TGGCCA 2 cut(s) 686, 1673
Mly113I GGCGCC 2 cut(s) 723, 1843
MlyI GAGTC 4 cut(s) 158, 379, 1186, 1861
MmeI TCCRAC 2 cut(s) 141, 1739
Mox20I TGGCCA 2 cut(s) 686, 1673
MroI TCCGGA 1 cut(s) 463
MroXI GAANNNNTTC 3 cut(s) 513, 1223, 1769
MscI TGGCCA 2 cut(s) 686, 1673
MseI TTAA 4 cut(s) 348, 903, 1281, 1626
MslI CAYNNNNRTG 1 cut(s) 581
Msp20I TGGCCA 2 cut(s) 686, 1673
MspA1I CMGCKG 1 cut(s) 1679
MspI CCGG 7 cut(s) 99, 464, 614, 882, 951, 981, 1590
Mva1269I GAATGC 1 cut(s) 1406
MvaI CCWGG 6 cut(s) 504, 544, 683, 764, 789, 2138
MvnI CGCG 1 cut(s) 1466
MwoI GCNNNNNNNGC 4 cut(s) 41, 371, 610, 1849
NarI GGCGCC 2 cut(s) 723, 1843
NciI CCSGG 4 cut(s) 99, 952, 982, 1591
NcoI CCATGG 1 cut(s) 1668
NlaIII CATG 9 cut(s) 16, 392, 433, 457, 824, 1507, 1672, 1693, 1757
NmuCI GTSAC 5 cut(s) 122, 805, 1107, 1581, 1741
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 2 cut(s) 1535, 2160
OliI CACNNNNGTG 1 cut(s) 581
PagI TCATGA 1 cut(s) 453
PciI ACATGT 1 cut(s) 429
PcsI WCGNNNNNNNCGW 2 cut(s) 745, 2157
PctI GAATGC 1 cut(s) 1406
PdmI GAANNNNTTC 3 cut(s) 513, 1223, 1769
PfeI GAWTC 4 cut(s) 48, 80, 1219, 1341
PflMI CCANNNNNTGG 1 cut(s) 1759
PkrI GCNGC 6 cut(s) 43, 69, 179, 818, 1212, 1635
PleI GAGTC 4 cut(s) 158, 379, 1185, 1861
PluTI GGCGCC 2 cut(s) 726, 1846
PpsI GAGTC 4 cut(s) 158, 379, 1185, 1861
Ppu21I YACGTR 1 cut(s) 1729
PscI ACATGT 1 cut(s) 429
PshAI GACNNNNGTC 1 cut(s) 1748
Psp6I CCWGG 6 cut(s) 502, 542, 681, 762, 787, 2136
PspEI GGTNACC 2 cut(s) 1107, 1581
PspGI CCWGG 6 cut(s) 502, 542, 681, 762, 787, 2136
PspOMI GGGCCC 1 cut(s) 474
PstI CTGCAG 1 cut(s) 1741
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 1711
RsaI GTAC 6 cut(s) 20, 1001, 1250, 1910, 1922, 2099
RsaNI GTAC 6 cut(s) 19, 1000, 1249, 1909, 1921, 2098
RseI CAYNNNNRTG 1 cut(s) 581
SaqAI TTAA 4 cut(s) 348, 903, 1281, 1626
SatI GCNGC 6 cut(s) 42, 68, 178, 817, 1211, 1634
ScaI AGTACT 1 cut(s) 1001
SchI GAGTC 4 cut(s) 158, 379, 1186, 1861
SduI GDGCHC 2 cut(s) 478, 1516
SexAI ACCWGGT 2 cut(s) 787, 2136
SfaNI GCATC 4 cut(s) 285, 586, 735, 832
SfcI CTRYAG 2 cut(s) 256, 1737
SfoI GGCGCC 2 cut(s) 724, 1844
SfuI TTCGAA 2 cut(s) 1535, 2160
SinI GGWCC 4 cut(s) 547, 663, 879, 1933
SmiMI CAYNNNNRTG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 341, 1954
SmoI CTYRAG 2 cut(s) 341, 1954
SnaBI TACGTA 1 cut(s) 1729
Sse9I AATT 7 cut(s) 54, 151, 183, 1122, 1376, 1398, 1443
SsiI CCGC 7 cut(s) 5, 41, 88, 572, 1210, 1351, 1677
SspDI GGCGCC 2 cut(s) 722, 1842
SspMI CTAG 3 cut(s) 671, 1274, 1637
StyI CCWWGG 4 cut(s) 324, 1478, 1668, 1847
TaaI ACNGT 5 cut(s) 260, 495, 522, 621, 691
TaiI ACGT 5 cut(s) 12, 243, 734, 1026, 1731
TaqI TCGA 9 cut(s) 72, 78, 234, 406, 769, 1535, 1653, 2121, 2160
TasI AATT 7 cut(s) 54, 151, 183, 1122, 1376, 1398, 1443
TatI WGTACW 3 cut(s) 999, 1248, 1908
TauI GCSGC 2 cut(s) 44, 1213
TfiI GAWTC 4 cut(s) 48, 80, 1219, 1341
Tru1I TTAA 4 cut(s) 348, 903, 1281, 1626
Tru9I TTAA 4 cut(s) 348, 903, 1281, 1626
TscAI CASTG 7 cut(s) 127, 527, 665, 694, 1327, 1746, 2024
TseFI GTSAC 5 cut(s) 122, 805, 1107, 1581, 1741
TseI GCWGC 4 cut(s) 67, 177, 816, 1633
Tsp45I GTSAC 5 cut(s) 122, 805, 1107, 1581, 1741
TspDTI ATGAA 8 cut(s) 442, 470, 837, 1053, 1091, 1436, 1588, 1678
TspGWI ACGGA 2 cut(s) 1058, 2085
TspRI CASTG 7 cut(s) 127, 527, 665, 694, 1327, 1746, 2024
Van91I CCANNNNNTGG 1 cut(s) 1759
VpaK11BI GGWCC 4 cut(s) 547, 663, 879, 1933
XceI RCATGY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 2 cut(s) 322, 550
XmiI GTMKAC 3 cut(s) 138, 497, 1062
XmnI GAANNNNTTC 3 cut(s) 513, 1223, 1769
XspI CTAG 3 cut(s) 671, 1274, 1637
ZraI GACGTC 1 cut(s) 10
ZrmI AGTACT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.