AT5G35730

SPX and EXS domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015825
Physical Location & Seq
Forward (+)
13893602 .. 13896976
3375 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35730.1

Sequence Viewer

Length: 1374 bp
ATGTTTGAAAATCCAGCTTCAAATAGTCCTCATCTTCGTAAATCTAGTAGCAAATCTGTCTTCATTGATCCTGGTTTAAACGGACATTTAGGTGAAATGGGTGACCTTAAAGGTTCAAATTCACCACTTCATATAACAACTATGGTTCCTTCTCCTATCTTCCTCTGGAGATTTAAGGTGGTTTTGTTTCTGTTTTGGGCTTTATGTTGCTGCAAGATTGGATGGGATTCAGTTATGAGAATGAGTATCGACCTGCGAGATTTATTTTTGTATGAAGCGTTTCTTTATTACAATCCTCTTCTTCTTGTGACAATGATGGTTTGGCTGTGGGGAGTGAACTTATGGGTGTTCTCTCAAGGAAGTGTTAATTATTCCAAAGTCTTTGATCTTGATCATAACCACCTTACTCATAGAGAGATGTGGAAGTGTAGCATGTGGATGACAATCATTGTCCCAACTAGCATGACAGCGTATCTATATCTGTATTCTCATGGGGAAGTATCTTTGGCTGCTTCACAACCGGTGCTCCTGTACATTGCTTTTGCTTTGGTACTGATATTTCCTTTCGATATTTTCTACTTGTCAAGTCGATATTTTCTGCTGAGAACATTATGGAGGATAGCTTTTCCACTGCAGCCAATTACATTTCCAGACTTCTTTTTGGCAGATATTCTGACCTCCATGGTAAAGGTCTTTTCCGACTTAGAGCGGTCCGTTTGCCGGATGGTGCATAGACAGGTCGCTACAATTGCTTGGTTTGAAGCTGACGCTGTCTGTGGCAGTCATCAAATTGCAATCCCATTGGTTCTTGTTTTCCCCTACATTTGCCGTCTTTTGCAATGTCTTCGACAATACAAAGATACAAAGGAAAAATCATCTCTATTAAATGCTCTGAAGTATTCTACAGCAGTACCCGTGATCTTCCTTTCTGCGCTTAAATATCACGTGATGCCCGAGAGCTGGACATCATTTTACCGACCGCTCTGGCTTTTCTCAAGTGTCATCAACTCACTGTACTCTTTCTACTGGGATGTAACTCGGGATTGGGACTTGAGCGGTTTCACTAAGATATTCAAGTTTAGCCGTCCAAGTACCATATCAAACTTATTATATGGCCGACAATGGGTATATTTCTGGGTGATAGGGAGCAATCTGGTGCTAAGATGCGCGTGGACATACAAATTATCAGCACATTTGAGGCACAACTACATAACGGTGTTCACAATGACAGCGATGGAGATGTTGAGACGATTTCAATGGGTATTTTTCAGAGTAGAGAATGAGTGGAACAAGATCACTAAATCACATCCAATGGGTGAGATTTCACTTGAAGAAGATAAACTACTTGGTTCTACTACACCTCATGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EXS

Protein Analysis

457

Amino Acids

53.56

Weight (kDa)

8.91

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 94 - 429 6e-95 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 261
AccBSI CCGCTC 3 cut(s) 709, 982, 1056
AccII CGCG 1 cut(s) 1169
AciI CCGC 3 cut(s) 709, 980, 1056
AclWI GGATC 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 1114
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 914
AcvI CACGTG 1 cut(s) 946
AfaI GTAC 5 cut(s) 533, 552, 912, 1016, 1093
AfiI CCNNNNNNNGG 3 cut(s) 720, 960, 1123
AgeI ACCGGT 1 cut(s) 520
AgsI TTSAA 7 cut(s) 8, 21, 117, 761, 1075, 1256, 1331
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 4 cut(s) 17, 623, 764, 960
AluI AGCT 4 cut(s) 17, 623, 764, 960
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 1 cut(s) 1240
AlwI GGATC 1 cut(s) 62
Ama87I CYCGRG 2 cut(s) 953, 1038
AoxI GGCC 1 cut(s) 1114
ApeKI GCWGC 3 cut(s) 210, 509, 634
ApoI RAATTY 1 cut(s) 118
AsiGI ACCGGT 1 cut(s) 520
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 2 cut(s) 934, 1169
AspS9I GGNCC 1 cut(s) 711
AsuHPI GGTGA 5 cut(s) 104, 113, 114, 1150, 1328
AvaI CYCGRG 2 cut(s) 953, 1038
AvaII GGWCC 1 cut(s) 711
BaeI ACNNNNGTAYC 2 cut(s) 894, 927
BarI GAAGNNNNNNTAC 2 cut(s) 1326, 1358
BbrPI CACGTG 1 cut(s) 946
BbsI GAAGAC 2 cut(s) 52, 836
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 197, 496, 646
BccI CCATC 4 cut(s) 216, 310, 718, 1228
BceAI ACGGC 2 cut(s) 813, 1068
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 1 cut(s) 72
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 1 cut(s) 1240
BfaI CTAG 2 cut(s) 45, 459
BfmI CTRYAG 2 cut(s) 632, 903
BfuAI ACCTGC 1 cut(s) 261
BisI GCNGC 3 cut(s) 211, 510, 635
BlsI GCNGC 3 cut(s) 212, 511, 636
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 711
BmeT110I CYCGRG 2 cut(s) 953, 1038
BmgT120I GGNCC 1 cut(s) 711
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 72
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 2 cut(s) 939, 1154
BmuI ACTGGG 1 cut(s) 1036
BpiI GAAGAC 2 cut(s) 52, 836
BpmI CTGGAG 1 cut(s) 187
BpuEI CTTGAG 3 cut(s) 339, 979, 1072
BsaAI YACGTR 1 cut(s) 946
BsaBI GATNNNNATC 2 cut(s) 390, 443
BsaJI CCNNGG 1 cut(s) 681
BsaWI WCCGGW 1 cut(s) 520
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 3 cut(s) 720, 960, 1123
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 1 cut(s) 1031
Bse3DI GCAATG 2 cut(s) 534, 845
Bse8I GATNNNNATC 2 cut(s) 390, 443
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 681
BseGI GGATG 5 cut(s) 227, 444, 729, 1036, 1306
BseJI GATNNNNATC 2 cut(s) 390, 443
BseLI CCNNNNNNNGG 3 cut(s) 720, 960, 1123
BseMI GCAATG 2 cut(s) 534, 845
BseMII CTCAG 1 cut(s) 593
BseNI ACTGG 1 cut(s) 1031
BseXI GCAGC 3 cut(s) 197, 496, 646
Bsh1236I CGCG 1 cut(s) 1169
Bsh1285I CGRYCG 1 cut(s) 980
BshFI GGCC 1 cut(s) 1116
BshTI ACCGGT 1 cut(s) 520
BsiEI CGRYCG 1 cut(s) 980
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 2 cut(s) 953, 1038
BsiSI CCGG 2 cut(s) 521, 721
BslFI GGGAC 2 cut(s) 437, 1061
BslI CCNNNNNNNGG 3 cut(s) 720, 960, 1123
BsmAI GTCTC 1 cut(s) 1240
BsmBI CGTCTC 1 cut(s) 1240
BsmFI GGGAC 2 cut(s) 437, 1061
BsnI GGCC 1 cut(s) 1116
BsoBI CYCGRG 2 cut(s) 953, 1038
Bsp1286I GDGCHC 1 cut(s) 528
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 5 cut(s) 67, 385, 391, 918, 1293
Bsp19I CCATGG 1 cut(s) 681
BspACI CCGC 3 cut(s) 709, 980, 1056
BspANI GGCC 1 cut(s) 1116
BspCNI CTCAG 1 cut(s) 594
BspFNI CGCG 1 cut(s) 1169
BspHI TCATGA 1 cut(s) 1363
BspLI GGNNCC 1 cut(s) 147
BspMAI CTGCAG 1 cut(s) 636
BspMI ACCTGC 1 cut(s) 261
BspPI GGATC 1 cut(s) 62
BsrBI CCGCTC 3 cut(s) 709, 982, 1056
BsrDI GCAATG 2 cut(s) 534, 845
BsrFI RCCGGY 1 cut(s) 520
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 1 cut(s) 1031
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 1 cut(s) 681
BssMI GATC 5 cut(s) 67, 385, 391, 918, 1293
BssT1I CCWWGG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 2 cut(s) 1014, 1216
Bst6I CTCTTC 1 cut(s) 303
BstAUI TGTACA 1 cut(s) 531
BstBAI YACGTR 1 cut(s) 946
BstDEI CTNAG 4 cut(s) 602, 703, 1065, 1160
BstDSI CCRYGG 1 cut(s) 681
BstEII GGTNACC 1 cut(s) 101
BstF5I GGATG 5 cut(s) 227, 444, 729, 1036, 1306
BstFNI CGCG 1 cut(s) 1169
BstHHI GCGC 2 cut(s) 934, 1169
BstKTI GATC 5 cut(s) 70, 388, 394, 921, 1296
BstMAI GTCTC 1 cut(s) 1240
BstMBI GATC 5 cut(s) 67, 385, 391, 918, 1293
BstMCI CGRYCG 1 cut(s) 980
BstMWI GCNNNNNNNGC 1 cut(s) 749
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 1 cut(s) 436
BstPI GGTNACC 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 2 cut(s) 632, 903
BstUI CGCG 1 cut(s) 1169
BstV1I GCAGC 3 cut(s) 197, 496, 646
BstV2I GAAGAC 2 cut(s) 52, 836
BsuRI GGCC 1 cut(s) 1116
BtgI CCRYGG 1 cut(s) 681
BtgZI GCGATG 1 cut(s) 1247
BtsCI GGATG 5 cut(s) 227, 444, 729, 1036, 1306
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 2 cut(s) 629, 1010
BveI ACCTGC 1 cut(s) 261
CciI TCATGA 1 cut(s) 1363
CfoI GCGC 2 cut(s) 934, 1169
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 711
CpoI CGGWCCG 1 cut(s) 711
CseI GACGC 1 cut(s) 776
Csp6I GTAC 5 cut(s) 532, 551, 911, 1015, 1092
CspAI ACCGGT 1 cut(s) 520
CspI CGGWCCG 1 cut(s) 711
CviAII CATG 5 cut(s) 433, 463, 491, 682, 1364
CviQI GTAC 5 cut(s) 532, 551, 911, 1015, 1092
DdeI CTNAG 4 cut(s) 602, 703, 1065, 1160
DpnI GATC 5 cut(s) 69, 387, 393, 920, 1295
DpnII GATC 5 cut(s) 67, 385, 391, 918, 1293
DraI TTTAAA 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 1114
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 681
Eco47I GGWCC 1 cut(s) 711
Eco57I CTGAAG 1 cut(s) 914
Eco72I CACGTG 1 cut(s) 946
Eco88I CYCGRG 2 cut(s) 953, 1038
Eco91I GGTNACC 1 cut(s) 101
EcoO65I GGTNACC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 1240
FaeI CATG 5 cut(s) 436, 466, 494, 685, 1367
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 2 cut(s) 437, 1061
FatI CATG 5 cut(s) 432, 462, 490, 681, 1363
FbaI TGATCA 1 cut(s) 391
Fnu4HI GCNGC 3 cut(s) 211, 510, 635
FokI GGATG 5 cut(s) 234, 451, 736, 1043, 1293
Fsp4HI GCNGC 3 cut(s) 211, 510, 635
FspBI CTAG 2 cut(s) 45, 459
GlaI GCGC 2 cut(s) 933, 1168
GluI GCNGC 3 cut(s) 211, 510, 635
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 1 cut(s) 1116
HapII CCGG 2 cut(s) 521, 721
HgaI GACGC 1 cut(s) 776
HhaI GCGC 2 cut(s) 934, 1169
Hin1II CATG 5 cut(s) 436, 466, 494, 685, 1367
Hin6I GCGC 2 cut(s) 932, 1167
HinP1I GCGC 2 cut(s) 932, 1167
HinfI GANTC 1 cut(s) 227
HpaII CCGG 2 cut(s) 521, 721
HphI GGTGA 5 cut(s) 104, 113, 114, 1150, 1328
Hpy166II GTNNAC 3 cut(s) 337, 1173, 1221
Hpy188I TCNGA 4 cut(s) 675, 700, 894, 1271
Hpy188III TCNNGA 5 cut(s) 166, 389, 650, 1040, 1364
Hpy8I GTNNAC 3 cut(s) 337, 1173, 1221
HpyAV CCTTC 1 cut(s) 159
HpyCH4III ACNGT 2 cut(s) 1014, 1216
HpyCH4IV ACGT 1 cut(s) 945
HpyCH4V TGCA 5 cut(s) 213, 634, 730, 794, 838
HpyF10VI GCNNNNNNNGC 1 cut(s) 749
HpyF3I CTNAG 4 cut(s) 602, 703, 1065, 1160
HpySE526I ACGT 1 cut(s) 945
Hsp92II CATG 5 cut(s) 436, 466, 494, 685, 1367
HspAI GCGC 2 cut(s) 932, 1167
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 5 cut(s) 67, 385, 391, 918, 1293
LmnI GCTCC 2 cut(s) 531, 1146
Lsp1109I GCAGC 3 cut(s) 197, 496, 646
LweI GCATC 2 cut(s) 939, 1154
MaeI CTAG 2 cut(s) 45, 459
MaeII ACGT 1 cut(s) 945
MaeIII GTNAC 3 cut(s) 101, 307, 1033
MalI GATC 5 cut(s) 69, 387, 393, 920, 1295
MbiI CCGCTC 3 cut(s) 709, 982, 1056
MboI GATC 5 cut(s) 67, 385, 391, 918, 1293
MboII GAAGA 9 cut(s) 26, 52, 151, 290, 293, 836, 913, 1343, 1346
MfeI CAATTG 1 cut(s) 747
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 6 cut(s) 118, 367, 639, 747, 789, 1181
MmeI TCCRAC 1 cut(s) 723
MnlI CCTC 7 cut(s) 39, 173, 306, 609, 688, 1191, 1371
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 6 cut(s) 77, 108, 174, 366, 884, 936
MslI CAYNNNNRTG 3 cut(s) 90, 437, 1214
MspI CCGG 2 cut(s) 521, 721
MspR9I CCNGG 1 cut(s) 72
MssI GTTTAAAC 1 cut(s) 78
MunI CAATTG 1 cut(s) 747
MvaI CCWGG 1 cut(s) 72
MvnI CGCG 1 cut(s) 1169
MwoI GCNNNNNNNGC 1 cut(s) 749
NcoI CCATGG 1 cut(s) 681
NdeII GATC 5 cut(s) 67, 385, 391, 918, 1293
NlaIII CATG 5 cut(s) 436, 466, 494, 685, 1367
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 101, 307
NspI RCATGY 1 cut(s) 436
PagI TCATGA 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 1 cut(s) 951
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 227
PflFI GACNNNGTC 1 cut(s) 770
PinAI ACCGGT 1 cut(s) 520
PkrI GCNGC 3 cut(s) 212, 511, 636
PmaCI CACGTG 1 cut(s) 946
PmeI GTTTAAAC 1 cut(s) 78
PmlI CACGTG 1 cut(s) 946
Ppu21I YACGTR 1 cut(s) 946
Psp6I CCWGG 1 cut(s) 70
PspCI CACGTG 1 cut(s) 946
PspEI GGTNACC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 1 cut(s) 711
PstI CTGCAG 1 cut(s) 636
PsyI GACNNNGTC 1 cut(s) 770
RsaI GTAC 5 cut(s) 533, 552, 912, 1016, 1093
RsaNI GTAC 5 cut(s) 532, 551, 911, 1015, 1092
RseI CAYNNNNRTG 3 cut(s) 90, 437, 1214
Rsr2I CGGWCCG 1 cut(s) 711
RsrII CGGWCCG 1 cut(s) 711
SaqAI TTAA 6 cut(s) 77, 108, 174, 366, 884, 936
SatI GCNGC 3 cut(s) 211, 510, 635
Sau3AI GATC 5 cut(s) 67, 385, 391, 918, 1293
Sau96I GGNCC 1 cut(s) 711
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 2 cut(s) 939, 1154
SfcI CTRYAG 2 cut(s) 632, 903
SinI GGWCC 1 cut(s) 711
SmiMI CAYNNNNRTG 3 cut(s) 90, 437, 1214
SmlI CTYRAG 3 cut(s) 354, 994, 1051
SmoI CTYRAG 3 cut(s) 354, 994, 1051
Sse9I AATT 6 cut(s) 118, 367, 639, 747, 789, 1181
SsiI CCGC 3 cut(s) 709, 980, 1056
SspMI CTAG 2 cut(s) 45, 459
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 2 cut(s) 1014, 1216
TaiI ACGT 1 cut(s) 948
TaqI TCGA 4 cut(s) 249, 567, 589, 847
TasI AATT 6 cut(s) 118, 367, 639, 747, 789, 1181
TatI WGTACW 2 cut(s) 531, 1014
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 6 cut(s) 77, 108, 174, 366, 884, 936
Tru9I TTAA 6 cut(s) 77, 108, 174, 366, 884, 936
TscAI CASTG 2 cut(s) 636, 1017
TseFI GTSAC 2 cut(s) 101, 307
TseI GCWGC 3 cut(s) 210, 509, 634
Tsp45I GTSAC 2 cut(s) 101, 307
TspDTI ATGAA 3 cut(s) 52, 119, 288
TspGWI ACGGA 2 cut(s) 96, 703
TspRI CASTG 2 cut(s) 636, 1017
Tth111I GACNNNGTC 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 711
XapI RAATTY 1 cut(s) 118
XceI RCATGY 1 cut(s) 436
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 2 cut(s) 45, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.