AT5G35750

histidine kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003045
Physical Location & Seq
Reverse (-)
13911283 .. 13916805
5523 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35750.1

Sequence Viewer

Length: 3531 bp
ATGTCTATAACTTGTGAGCTCTTGAATCTTACTTCAAAGAAAGCTAAGAAGTCGTCGAGCAGTGACAAGAAATGGCTAAAGAAGCCTCTCTTCTTCCTGATTTTGTGTGGCTCTTTGGTAATTGTTTTGGTTATGTTCTTACGGTTAGGTAGAAGTCAGAAGGAGGAGACAGATTCTTGTAATGGAGAAGAGAAAGTGTTGTATAGACATCAAAATGTCACAAGAAGTGAGATTCATGATTTGGTCTCTTTGTTCTCTGATTCAGATCAGGTAACATCCTTTGAATGTCATAAGGAATCAAGCCCTGGAATGTGGACAAACTATGGTATTACATGTTCCCTGAGTGTGCGTTCTGATAAACAAGAGACTAGAGGGCTTCCCTGGAATCTTGGCTTAGGACATTCTATCTCATCAACATCTTGTATGTGTGGTAATCTTGAACCGATTTTACAGCAACCTGAAAACCTTGAGGAAGAAAACCATGAAGAAGGGCTGGAGCAGGGTTTGTCATCGTATTTAAGAAATGCATGGTGGTGTCTAATCCTTGGTGTGTTAGTGTGCCATAAGATTTATGTATCTCATTCTAAAGCACGAGGTGAGAGGAAAGAGAAAGTACATCTGCAAGAGGCTTTAGCTCCAAAGAAGCAGCAACAACGTGCTCAGACTTCTTCTAGAGGGGCTGGAAGATGGAGGAAGAATATCCTTCTCCTTGGTATTTTAGGAGGAGTTTCCTTCTCTGTTTGGTGGTTTTGGGACACTAATGAGGAGATCATAATGAAAAGGAGGGAGACTTTGGCAAACATGTGTGACGAACGAGCACGTGTTTTACAAGATCAGTTCAATGTTAGCTTGAACCATGTTCATGCCTTGTCTATTCTTGTATCTACATTTCATCATGGTAAAATCCCATCTGCCATTGATCAGAGAACATTTGAAGAATATACTGAGAGAACAAACTTTGAGAGGCCACTTACTAGTGGTGTAGCGTATGCTTTGAAAGTCCCACACTCAGAAAGAGAGAAATTTGAAAAGGAGCATGGATGGGCAATAAAGAAAATGGAAACTGAGGACCAGACAGTTGTACAAGATTGTGTTCCTGAAAACTTTGATCCCGCACCGATTCAAGACGAATACGCGCCAGTTATATTTGCTCAAGAAACTGTTTCCCATATTGTATCGGTCGACATGATGTCTGGAGAAGAAGACCGTGAAAACATCTTACGGGCAAGGGCATCAGGAAAAGGAGTGTTAACATCTCCATTTAAGCTTCTTAAGTCAAATCATCTTGGTGTTGTGTTGACCTTTGCTGTCTATGACACGAGCCTACCGCCTGATGCTACAGAAGAACAGCGTGTTGAAGCAACTATTGGGTACCTTGGTGCATCATATGATATGCCATCGCTGGTGGAGAAACTTCTTCACCAACTTGCCAGCAAACAGACAATTGCTGTGGATGTTTACGACACAACTAACACTTCAGGTCTAATAAAAATGTATGGCTCAGAAATTGGGGATATAAGTGAGCAGCATATAAGTAGCCTTGATTTTGGTGATCCATCAAGGAACCATGAGATGCATTGCAGGTTTAAGCATAAACTTCCCATTCCCTGGACAGCGATAACACCGTCGATCTTAGTTCTGGTTATTACTTTTCTTGTTGGTTATATTTTATATGAAGCCATCAACCGAATTGCGACAGTTGAAGAGGATTGTCAGAAGATGAGGGAACTCAAAGCTCGTGCTGAGGCCGCTGACATTGCAAAGTCACAGTTCCTAGCAACTGTTTCTCATGAGATACGGACTCCGATGAATGGAGTTTTAGGAATGCTGAAAATGCTGATGGACACCGATCTTGATGCGAAGCAGATGGACTATGCGCAAACTGCTCATGGCAGTGGGAAGGATCTTACATCACTAATAAATGAGGTTCTTGATCAGGCAAAGATTGAATCCGGAAGGCTCGAGCTTGAAAATGTGCCTTTTGATATGCGTTTTATTCTTGATAATGTTTCATCTCTCCTCTCTGGCAAGGCAAATGAAAAAGGAATTGAGTTGGCCGTTTATGTTTCTAGTCAAGTTCCTGATGTTGTAGTCGGTGATCCGAGTCGGTTCCGGCAGATCATTACAAACCTGGTTGGAAACTCAATCAAATTCACACAGGAAAGGGGACACATATTTATCTCAGTGCACCTTGCAGATGAGGTAAAGGAGCCTCTTACTATTGAAGACGCAGTGCTAAAACAGCGACTAGCTTTAGGATGCAGCGAGTCCGGTGAGACAGTTAGCGGGTTTCCTGCGGTAAATGCATGGGGAAGCTGGAAGAATTTCAAGACATGTTACAGTACTGAGAGTCAGAATTCTGATCAAATCAAATTGCTAGTTACAGTGGAGGACACTGGAGTTGGCATACCTGTGGATGCACAAGGCCGAATCTTCACACCTTTTATGCAAGCCGACAGTTCCACATCGCGGACTTATGGTGGAACTGGCATAGGTTTGAGTATAAGCAAACGTTTGGTTGAACTCATGCAAGGAGAGATGGGGTTTGTGAGTGAGCCCGGGATAGGCAGTACTTTTTCATTTACTGGAGTTTTCGGGAAAGCAGAAACAAATACGTCGATTACTAAGCTGGAACGATTCGATCTAGCTATTCAGGAGTTTACAGGATTGAGAGCATTAGTTATTGATAACAGAAACATTAGAGCAGAGGTCACCAGGTACGAACTTCGGAGACTGGGAATATCTGCAGACATTGTTTCAAGTCTGAGAATGGCATGCACTTGTTGTATCAGCAAATTAGAAAATTTGGCTATGATTCTAATAGACAAAGACGCCTGGAACAAGGAAGAATTTTCAGTACTTGACGAGTTGTTTACCCGAAGCAAAGTAACCTTTACAAGAGTCCCAAAGATTTTTCTTTTGGCAACTTCTGCAACTCTTACTGAGCGCAGTGAGATGAAGTCTACTGGTCTCATCGATGAGGTGGTGATAAAGCCTCTTCGGATGAGTGTCTTAATATGTTGCTTGCAAGAAACCCTTGTCAATGGCAAGAAGAGGCAACCGAACAGACAGCGAAGAAATCTTGGACACTTGCTAAGAGAAAAACAGATTCTGGTTGTGGATGATAATCTTGTGAACAGACGAGTTGCAGAAGGTGCACTTAAGAAATATGGAGCTATTGTTACATGCGTTGAGAGTGGCAAAGCTGCATTGGCAATGCTTAAGCCGCCTCATAACTTCGATGCTTGCTTCATGGATCTCCAGATGCCTGAAATGGATGGATTTGAAGCGACAAGGAGAGTCCGTGAGCTGGAGAGGGAAATCAATAAGAAAATAGCTTCTGGAGAAGTTTCAGCTGAAATGTTCTGTAAATTTAGTAGTTGGCACGTCCCGATATTAGCAATGACAGCAGATGTTATTCAGGCTACTCATGAAGAATGCATGAAATGTGGAATGGATGGTTATGTATCAAAACCGTTTGAAGAGGAAGTGCTCTACACAGCGGTAGCAAGATTCTTTGAACCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1176

Amino Acids

131.86

Weight (kDa)

5.9

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 305 - 496 1.1e-37 CHASE domain
HisKA PF00512 587 - 652 7.9e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 790 - 862 1.1e-17 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 885 - 1013 9.8e-33 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 1037 - 1169 3.1e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1878
Acc36I ACCTGC 1 cut(s) 1572
Acc65I GGTACC 1 cut(s) 1371
AccB1I GGYRCC 1 cut(s) 1371
AccB7I CCANNNNNTGG 1 cut(s) 1608
AccI GTMKAC 2 cut(s) 1182, 2963
AccII CGCG 2 cut(s) 1136, 2470
AccIII TCCGGA 1 cut(s) 1952
AciI CCGC 8 cut(s) 1113, 1328, 1749, 2286, 2297, 2470, 3227, 3503
AclI AACGTT 1 cut(s) 2512
AclWI GGATC 5 cut(s) 1103, 1547, 1911, 2093, 3264
AcoI YGGCCR 1 cut(s) 2055
AcsI RAATTY 7 cut(s) 1022, 2150, 2323, 2356, 2803, 2849, 3371
AcuI CTGAAG 1 cut(s) 1461
AcvI CACGTG 1 cut(s) 821
AcyI GRCGYC 1 cut(s) 2832
AdeI CACNNNGTG 1 cut(s) 596
AfaI GTAC 7 cut(s) 615, 1083, 1373, 2344, 2572, 2720, 2859
AfiI CCNNNNNNNGG 5 cut(s) 1608, 1811, 2469, 2564, 3310
AflII CTTAAG 3 cut(s) 1271, 3161, 3221
AflIII ACRYGT 4 cut(s) 332, 801, 820, 2333
AhdI GACNNNNNGTC 1 cut(s) 1189
AhlI ACTAGT 1 cut(s) 974
AjiI CACGTC 1 cut(s) 3388
AjnI CCWGG 6 cut(s) 304, 380, 1607, 2130, 2714, 2834
AjuI GAANNNNNNNTTGG 2 cut(s) 1350, 1382
Alw21I GWGCWC 6 cut(s) 21, 661, 820, 2190, 3160, 3495
Alw26I GTCTC 7 cut(s) 161, 250, 359, 782, 2270, 2725, 2975
Alw44I GTGCAC 2 cut(s) 2186, 3156
AlwI GGATC 5 cut(s) 1103, 1547, 1911, 2093, 3264
AlwNI CAGNNNCTG 1 cut(s) 3112
Ama87I CYCGRG 2 cut(s) 1961, 2558
Aor13HI TCCGGA 1 cut(s) 1952
AoxI GGCC 4 cut(s) 965, 1746, 2055, 2425
ApaLI GTGCAC 2 cut(s) 2186, 3156
ApeKI GCWGC 4 cut(s) 646, 1525, 2262, 3206
ApoI RAATTY 7 cut(s) 1022, 2150, 2323, 2356, 2803, 2849, 3371
Asp700I GAANNNNTTC 1 cut(s) 2324
Asp718I GGTACC 1 cut(s) 1371
AspLEI GCGC 3 cut(s) 1138, 1879, 2949
AspS9I GGNCC 1 cut(s) 1069
AsuC2I CCSGG 2 cut(s) 2559, 2560
AsuHPI GGTGA 7 cut(s) 608, 1412, 1562, 2108, 2285, 2704, 2998
AvaI CYCGRG 2 cut(s) 1961, 2558
AvaII GGWCC 1 cut(s) 1069
BaeGI GKGCMC 2 cut(s) 2190, 3160
BaeI ACNNNNGTAYC 2 cut(s) 1363, 1396
BanI GGYRCC 1 cut(s) 1371
BanII GRGCYC 2 cut(s) 21, 2559
BauI CACGAG 3 cut(s) 591, 1318, 1737
BbrPI CACGTG 1 cut(s) 821
BbsI GAAGAC 2 cut(s) 1209, 2232
Bbv12I GWGCWC 6 cut(s) 21, 661, 820, 2190, 3160, 3495
BbvCI CCTCAGC 1 cut(s) 1743
BbvI GCAGC 4 cut(s) 658, 1537, 2274, 3193
BceAI ACGGC 1 cut(s) 2042
BcgI CGANNNNNNTGC 2 cut(s) 1838, 1872
BciT130I CCWGG 6 cut(s) 306, 382, 1609, 2132, 2716, 2836
BclI TGATCA 3 cut(s) 919, 1933, 2362
BcnI CCSGG 2 cut(s) 2559, 2560
BcoDI GTCTC 7 cut(s) 161, 250, 359, 782, 2270, 2725, 2975
BcuI ACTAGT 1 cut(s) 974
BfaI CTAG 8 cut(s) 369, 672, 975, 1775, 2070, 2249, 2378, 2645
BfmI CTRYAG 2 cut(s) 1338, 2745
BfrI CTTAAG 3 cut(s) 1271, 3161, 3221
BfuAI ACCTGC 1 cut(s) 1572
BisI GCNGC 6 cut(s) 647, 1526, 1749, 2263, 3207, 3227
BlsI GCNGC 6 cut(s) 648, 1527, 1750, 2264, 3208, 3228
BmcAI AGTACT 3 cut(s) 2344, 2572, 2859
Bme1390I CCNGG 8 cut(s) 306, 382, 1609, 2132, 2559, 2560, 2716, 2836
Bme18I GGWCC 1 cut(s) 1069
BmeRI GACNNNNNGTC 1 cut(s) 1189
BmeT110I CYCGRG 2 cut(s) 1961, 2558
BmgBI CACGTC 1 cut(s) 3388
BmgT120I GGNCC 1 cut(s) 1069
BmiI GGNNCC 4 cut(s) 1373, 1565, 2111, 2211
BmrFI CCNGG 8 cut(s) 306, 382, 1609, 2132, 2559, 2560, 2716, 2836
BmrI ACTGGG 1 cut(s) 2744
BmsI GCATC 9 cut(s) 1241, 1324, 1391, 1563, 1846, 2249, 2407, 3232, 3255
BmuI ACTGGG 1 cut(s) 2744
BpiI GAAGAC 2 cut(s) 1209, 2232
BpmI CTGGAG 7 cut(s) 515, 1215, 2418, 2607, 3245, 3332, 3363
Bpu10I CCTNAGC 2 cut(s) 394, 1743
BpuEI CTTGAG 2 cut(s) 488, 1137
BpuMI CCSGG 2 cut(s) 2559, 2560
Bsa29I ATCGAT 1 cut(s) 2976
BsaAI YACGTR 1 cut(s) 821
BsaBI GATNNNNATC 2 cut(s) 264, 3126
BsaHI GRCGYC 1 cut(s) 2832
BsaI GGTCTC 2 cut(s) 250, 2975
BsaJI CCNNGG 7 cut(s) 304, 380, 544, 709, 1375, 1607, 2558
BsaWI WCCGGW 2 cut(s) 1952, 2270
BsaXI ACNNNNNCTCC 4 cut(s) 3165, 3195, 3289, 3319
Bsc4I CCNNNNNNNGG 5 cut(s) 1608, 1811, 2469, 2564, 3310
Bse1I ACTGG 6 cut(s) 1139, 2401, 2491, 2590, 2739, 2971
Bse3DI GCAATG 4 cut(s) 1576, 1755, 3222, 3408
Bse8I GATNNNNATC 2 cut(s) 264, 3126
BseAI TCCGGA 1 cut(s) 1952
BseBI CCWGG 6 cut(s) 306, 382, 1609, 2132, 2716, 2836
BseCI ATCGAT 1 cut(s) 2976
BseDI CCNNGG 7 cut(s) 304, 380, 544, 709, 1375, 1607, 2558
BseGI GGATG 9 cut(s) 275, 1046, 1459, 2264, 2422, 3009, 3127, 3283, 3463
BseJI GATNNNNATC 2 cut(s) 264, 3126
BseLI CCNNNNNNNGG 5 cut(s) 1608, 1811, 2469, 2564, 3310
BseMI GCAATG 4 cut(s) 1576, 1755, 3222, 3408
BseNI ACTGG 6 cut(s) 1139, 2401, 2491, 2590, 2739, 2971
BseRI GAGGAG 4 cut(s) 179, 738, 779, 2009
BseSI GKGCMC 2 cut(s) 2190, 3160
BseXI GCAGC 4 cut(s) 658, 1537, 2274, 3193
Bsh1236I CGCG 2 cut(s) 1136, 2470
Bsh1285I CGRYCG 1 cut(s) 1182
BshFI GGCC 4 cut(s) 967, 1748, 2057, 2427
BshNI GGYRCC 1 cut(s) 1371
BshVI ATCGAT 1 cut(s) 2976
BsiEI CGRYCG 1 cut(s) 1182
BsiHKAI GWGCWC 6 cut(s) 21, 661, 820, 2190, 3160, 3495
BsiHKCI CYCGRG 2 cut(s) 1961, 2558
BsiSI CCGG 4 cut(s) 1953, 2113, 2271, 2559
BslFI GGGAC 5 cut(s) 767, 986, 2181, 2888, 3374
BslI CCNNNNNNNGG 5 cut(s) 1608, 1811, 2469, 2564, 3310
BsmAI GTCTC 7 cut(s) 161, 250, 359, 782, 2270, 2725, 2975
BsmFI GGGAC 5 cut(s) 767, 986, 2181, 2888, 3374
BsmI GAATGC 2 cut(s) 1830, 3443
BsnI GGCC 4 cut(s) 967, 1748, 2057, 2427
Bso31I GGTCTC 2 cut(s) 250, 2975
BsoBI CYCGRG 2 cut(s) 1961, 2558
Bsp1286I GDGCHC 7 cut(s) 21, 661, 820, 2190, 2559, 3160, 3495
Bsp13I TCCGGA 1 cut(s) 1952
Bsp1407I TGTACA 1 cut(s) 1081
BspACI CCGC 8 cut(s) 1113, 1328, 1749, 2286, 2297, 2470, 3227, 3503
BspANI GGCC 4 cut(s) 967, 1748, 2057, 2427
BspDI ATCGAT 1 cut(s) 2976
BspEI TCCGGA 1 cut(s) 1952
BspFNI CGCG 2 cut(s) 1136, 2470
BspHI TCATGA 3 cut(s) 235, 1789, 3430
BspLI GGNNCC 4 cut(s) 1373, 1565, 2111, 2211
BspMAI CTGCAG 1 cut(s) 2749
BspMI ACCTGC 1 cut(s) 1572
BspPI GGATC 5 cut(s) 1103, 1547, 1911, 2093, 3264
BspT107I GGYRCC 1 cut(s) 1371
BspTI CTTAAG 3 cut(s) 1271, 3161, 3221
BspTNI GGTCTC 2 cut(s) 250, 2975
BsrDI GCAATG 4 cut(s) 1576, 1755, 3222, 3408
BsrGI TGTACA 1 cut(s) 1081
BsrI ACTGG 6 cut(s) 1139, 2401, 2491, 2590, 2739, 2971
BssECI CCNNGG 7 cut(s) 304, 380, 544, 709, 1375, 1607, 2558
BssNI GRCGYC 1 cut(s) 2832
BssSI CACGAG 3 cut(s) 591, 1318, 1737
BssT1I CCWWGG 3 cut(s) 544, 709, 1375
Bst2BI CACGAG 3 cut(s) 591, 1318, 1737
Bst2UI CCWGG 6 cut(s) 306, 382, 1609, 2132, 2716, 2836
Bst6I CTCTTC 6 cut(s) 95, 183, 1698, 3003, 3047, 3477
BstACI GRCGYC 1 cut(s) 2832
BstAFI CTTAAG 3 cut(s) 1271, 3161, 3221
BstAPI GCANNNNNTGC 2 cut(s) 2930, 3155
BstAUI TGTACA 1 cut(s) 1081
BstBAI YACGTR 1 cut(s) 821
BstC8I GCNNGC 5 cut(s) 1432, 2451, 2776, 3026, 3247
BstEII GGTNACC 1 cut(s) 2710
BstF5I GGATG 9 cut(s) 275, 1046, 1459, 2264, 2422, 3009, 3127, 3283, 3463
BstFNI CGCG 2 cut(s) 1136, 2470
BstHHI GCGC 3 cut(s) 1138, 1879, 2949
BstMAI GTCTC 7 cut(s) 161, 250, 359, 782, 2270, 2725, 2975
BstMCI CGRYCG 1 cut(s) 1182
BstNI CCWGG 6 cut(s) 306, 382, 1609, 2132, 2716, 2836
BstNSI RCATGY 5 cut(s) 336, 805, 2337, 2778, 3189
BstPI GGTNACC 1 cut(s) 2710
BstSCI CCNGG 8 cut(s) 304, 380, 1607, 2130, 2557, 2558, 2714, 2834
BstSFI CTRYAG 2 cut(s) 1338, 2745
BstSLI GKGCMC 2 cut(s) 2190, 3160
BstUI CGCG 2 cut(s) 1136, 2470
BstV1I GCAGC 4 cut(s) 658, 1537, 2274, 3193
BstV2I GAAGAC 2 cut(s) 1209, 2232
BstX2I RGATCY 2 cut(s) 1903, 3256
BstYI RGATCY 2 cut(s) 1903, 3256
Bsu15I ATCGAT 1 cut(s) 2976
BsuRI GGCC 4 cut(s) 967, 1748, 2057, 2427
BsuTUI ATCGAT 1 cut(s) 2976
BtgZI GCGATG 2 cut(s) 1383, 2451
BtrI CACGTC 1 cut(s) 3388
BtsCI GGATG 9 cut(s) 275, 1046, 1459, 2264, 2422, 3009, 3127, 3283, 3463
BtsI GCAGTG 4 cut(s) 67, 1900, 2238, 2956
BtsIMutI CAGTG 7 cut(s) 67, 1900, 2190, 2238, 2391, 2394, 2956
BveI ACCTGC 1 cut(s) 1572
Cac8I GCNNGC 5 cut(s) 1432, 2451, 2776, 3026, 3247
CaiI CAGNNNCTG 1 cut(s) 3112
CciI TCATGA 3 cut(s) 235, 1789, 3430
CfoI GCGC 3 cut(s) 1138, 1879, 2949
Cfr13I GGNCC 1 cut(s) 1069
Cfr9I CCCGGG 1 cut(s) 2558
ClaI ATCGAT 1 cut(s) 2976
CseI GACGC 2 cut(s) 2237, 2840
CsiI ACCWGGT 2 cut(s) 2130, 2714
Csp6I GTAC 7 cut(s) 614, 1082, 1372, 2343, 2571, 2719, 2858
CspCI CAANNNNNGTGG 2 cut(s) 1431, 1466
CviQI GTAC 7 cut(s) 614, 1082, 1372, 2343, 2571, 2719, 2858
DraIII CACNNNGTG 1 cut(s) 596
DriI GACNNNNNGTC 1 cut(s) 1189
EaeI YGGCCR 1 cut(s) 2055
Eam1104I CTCTTC 6 cut(s) 95, 183, 1698, 3003, 3047, 3477
Eam1105I GACNNNNNGTC 1 cut(s) 1189
EarI CTCTTC 6 cut(s) 95, 183, 1698, 3003, 3047, 3477
Ecl136II GAGCTC 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 544, 709, 1375
Eco24I GRGCYC 2 cut(s) 21, 2559
Eco31I GGTCTC 2 cut(s) 250, 2975
Eco47I GGWCC 1 cut(s) 1069
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 1461
Eco72I CACGTG 1 cut(s) 821
Eco88I CYCGRG 2 cut(s) 1961, 2558
Eco91I GGTNACC 1 cut(s) 2710
EcoICRI GAGCTC 1 cut(s) 19
EcoO65I GGTNACC 1 cut(s) 2710
EcoRI GAATTC 1 cut(s) 2356
EcoRII CCWGG 6 cut(s) 304, 380, 1607, 2130, 2714, 2834
EcoT14I CCWWGG 3 cut(s) 544, 709, 1375
EcoT22I ATGCAT 4 cut(s) 529, 1578, 2308, 3443
EcoT38I GRGCYC 2 cut(s) 21, 2559
ErhI CCWWGG 3 cut(s) 544, 709, 1375
FalI AAGNNNNNCTT 6 cut(s) 74, 106, 3021, 3053, 3144, 3176
FaqI GGGAC 5 cut(s) 767, 986, 2181, 2888, 3374
FauI CCCGC 2 cut(s) 1120, 2279
FauNDI CATATG 1 cut(s) 1387
FbaI TGATCA 3 cut(s) 919, 1933, 2362
FblI GTMKAC 2 cut(s) 1182, 2963
Fnu4HI GCNGC 6 cut(s) 647, 1526, 1749, 2263, 3207, 3227
FokI GGATG 9 cut(s) 262, 1053, 1466, 2271, 2429, 3016, 3134, 3290, 3470
FriOI GRGCYC 2 cut(s) 21, 2559
Fsp4HI GCNGC 6 cut(s) 647, 1526, 1749, 2263, 3207, 3227
FspBI CTAG 8 cut(s) 369, 672, 975, 1775, 2070, 2249, 2378, 2645
FspI TGCGCA 1 cut(s) 1878
GlaI GCGC 3 cut(s) 1137, 1878, 2948
GluI GCNGC 6 cut(s) 647, 1526, 1749, 2263, 3207, 3227
GsuI CTGGAG 7 cut(s) 515, 1215, 2418, 2607, 3245, 3332, 3363
HaeIII GGCC 4 cut(s) 967, 1748, 2057, 2427
HapII CCGG 4 cut(s) 1953, 2113, 2271, 2559
HgaI GACGC 2 cut(s) 2237, 2840
HhaI GCGC 3 cut(s) 1138, 1879, 2949
Hin1I GRCGYC 1 cut(s) 2832
Hin6I GCGC 3 cut(s) 1136, 1877, 2947
HinP1I GCGC 3 cut(s) 1136, 1877, 2947
HincII GTYRAC 3 cut(s) 1183, 1251, 1299
HindII GTYRAC 3 cut(s) 1183, 1251, 1299
HindIII AAGCTT 1 cut(s) 1265
HpaI GTTAAC 1 cut(s) 1251
HpaII CCGG 4 cut(s) 1953, 2113, 2271, 2559
HphI GGTGA 7 cut(s) 608, 1412, 1562, 2108, 2285, 2704, 2998
Hpy99I CGWCG 3 cut(s) 58, 1630, 2620
HpyAV CCTTC 7 cut(s) 154, 482, 713, 742, 1894, 1950, 3146
HpyCH4IV ACGT 5 cut(s) 655, 820, 2512, 2615, 3387
HpySE526I ACGT 5 cut(s) 655, 820, 2512, 2615, 3387
Hsp92I GRCGYC 1 cut(s) 2832
HspAI GCGC 3 cut(s) 1136, 1877, 2947
Kpn2I TCCGGA 1 cut(s) 1952
KpnI GGTACC 1 cut(s) 1375
Ksp22I TGATCA 3 cut(s) 919, 1933, 2362
KspAI GTTAAC 1 cut(s) 1251
LmnI GCTCC 5 cut(s) 496, 640, 1033, 2209, 3173
Lsp1109I GCAGC 4 cut(s) 658, 1537, 2274, 3193
LweI GCATC 9 cut(s) 1241, 1324, 1391, 1563, 1846, 2249, 2407, 3232, 3255
MabI ACCWGGT 2 cut(s) 2130, 2714
MaeI CTAG 8 cut(s) 369, 672, 975, 1775, 2070, 2249, 2378, 2645
MaeII ACGT 5 cut(s) 655, 820, 2512, 2615, 3387
MfeI CAATTG 1 cut(s) 1443
MflI RGATCY 2 cut(s) 1903, 3256
MhlI GDGCHC 7 cut(s) 21, 661, 820, 2190, 2559, 3160, 3495
MlyI GAGTC 6 cut(s) 1795, 2113, 2276, 2359, 2910, 3309
MmeI TCCRAC 1 cut(s) 2116
Mph1103I ATGCAT 4 cut(s) 529, 1578, 2308, 3443
MroI TCCGGA 1 cut(s) 1952
MroXI GAANNNNTTC 1 cut(s) 2324
MseI TTAA 9 cut(s) 518, 1250, 1263, 1272, 1587, 3014, 3162, 3222, 3529
MslI CAYNNNNRTG 6 cut(s) 213, 532, 861, 1287, 1893, 2411
MspA1I CMGCKG 3 cut(s) 1751, 3356, 3503
MspCI CTTAAG 3 cut(s) 1271, 3161, 3221
MspI CCGG 4 cut(s) 1953, 2113, 2271, 2559
MspR9I CCNGG 8 cut(s) 306, 382, 1609, 2132, 2559, 2560, 2716, 2836
MunI CAATTG 1 cut(s) 1443
Mva1269I GAATGC 2 cut(s) 1830, 3443
MvaI CCWGG 6 cut(s) 306, 382, 1609, 2132, 2716, 2836
MvnI CGCG 2 cut(s) 1136, 2470
NciI CCSGG 2 cut(s) 2559, 2560
NdeI CATATG 1 cut(s) 1387
NlaIV GGNNCC 4 cut(s) 1373, 1565, 2111, 2211
NmuCI GTSAC 5 cut(s) 62, 217, 806, 1764, 2710
NsbI TGCGCA 1 cut(s) 1878
NsiI ATGCAT 4 cut(s) 529, 1578, 2308, 3443
NspI RCATGY 5 cut(s) 336, 805, 2337, 2778, 3189
PaeI GCATGC 1 cut(s) 2778
PaeR7I CTCGAG 1 cut(s) 1961
PagI TCATGA 3 cut(s) 235, 1789, 3430
PciI ACATGT 3 cut(s) 332, 801, 2333
PctI GAATGC 2 cut(s) 1830, 3443
PdmI GAANNNNTTC 1 cut(s) 2324
PflMI CCANNNNNTGG 1 cut(s) 1608
PkrI GCNGC 6 cut(s) 648, 1527, 1750, 2264, 3208, 3228
PleI GAGTC 6 cut(s) 1795, 2112, 2275, 2358, 2909, 3308
PmaCI CACGTG 1 cut(s) 821
PmlI CACGTG 1 cut(s) 821
PpsI GAGTC 6 cut(s) 1795, 2112, 2275, 2358, 2909, 3308
Ppu21I YACGTR 1 cut(s) 821
PscI ACATGT 3 cut(s) 332, 801, 2333
Psp124BI GAGCTC 1 cut(s) 21
Psp1406I AACGTT 1 cut(s) 2512
Psp6I CCWGG 6 cut(s) 304, 380, 1607, 2130, 2714, 2834
PspCI CACGTG 1 cut(s) 821
PspEI GGTNACC 1 cut(s) 2710
PspGI CCWGG 6 cut(s) 304, 380, 1607, 2130, 2714, 2834
PspN4I GGNNCC 4 cut(s) 1373, 1565, 2111, 2211
PspPI GGNCC 1 cut(s) 1069
PspXI VCTCGAGB 1 cut(s) 1961
PsrI GAACNNNNNNTAC 4 cut(s) 319, 351, 432, 464
PstI CTGCAG 1 cut(s) 2749
PstNI CAGNNNCTG 1 cut(s) 3112
PsuI RGATCY 2 cut(s) 1903, 3256
PvuII CAGCTG 1 cut(s) 3356
RsaI GTAC 7 cut(s) 615, 1083, 1373, 2344, 2572, 2720, 2859
RsaNI GTAC 7 cut(s) 614, 1082, 1372, 2343, 2571, 2719, 2858
RseI CAYNNNNRTG 6 cut(s) 213, 532, 861, 1287, 1893, 2411
SacI GAGCTC 1 cut(s) 21
SalI GTCGAC 1 cut(s) 1181
SaqAI TTAA 9 cut(s) 518, 1250, 1263, 1272, 1587, 3014, 3162, 3222, 3529
SatI GCNGC 6 cut(s) 647, 1526, 1749, 2263, 3207, 3227
Sau96I GGNCC 1 cut(s) 1069
ScaI AGTACT 3 cut(s) 2344, 2572, 2859
SchI GAGTC 6 cut(s) 1795, 2113, 2276, 2359, 2910, 3309
ScrFI CCNGG 8 cut(s) 306, 382, 1609, 2132, 2559, 2560, 2716, 2836
SduI GDGCHC 7 cut(s) 21, 661, 820, 2190, 2559, 3160, 3495
SexAI ACCWGGT 2 cut(s) 2130, 2714
SfaNI GCATC 9 cut(s) 1241, 1324, 1391, 1563, 1846, 2249, 2407, 3232, 3255
SfcI CTRYAG 2 cut(s) 1338, 2745
Sfr274I CTCGAG 1 cut(s) 1961
SinI GGWCC 1 cut(s) 1069
SlaI CTCGAG 1 cut(s) 1961
SmaI CCCGGG 1 cut(s) 2560
SmiMI CAYNNNNRTG 6 cut(s) 213, 532, 861, 1287, 1893, 2411
SmlI CTYRAG 6 cut(s) 467, 1152, 1271, 1961, 3161, 3221
SmoI CTYRAG 6 cut(s) 467, 1152, 1271, 1961, 3161, 3221
SpeI ACTAGT 1 cut(s) 974
SphI GCATGC 1 cut(s) 2778
SsiI CCGC 8 cut(s) 1113, 1328, 1749, 2286, 2297, 2470, 3227, 3503
SspMI CTAG 8 cut(s) 369, 672, 975, 1775, 2070, 2249, 2378, 2645
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 8 cut(s) 304, 380, 1607, 2130, 2557, 2558, 2714, 2834
StyI CCWWGG 3 cut(s) 544, 709, 1375
TaiI ACGT 5 cut(s) 658, 823, 2515, 2618, 3390
TaqI TCGA 8 cut(s) 56, 1182, 1628, 1962, 2618, 2640, 2976, 3240
TaqII GACCGA 1 cut(s) 1168
TatI WGTACW 5 cut(s) 613, 1081, 2342, 2570, 2857
TauI GCSGC 2 cut(s) 1751, 3229
Tru1I TTAA 9 cut(s) 518, 1250, 1263, 1272, 1587, 3014, 3162, 3222, 3529
Tru9I TTAA 9 cut(s) 518, 1250, 1263, 1272, 1587, 3014, 3162, 3222, 3529
TscAI CASTG 7 cut(s) 67, 1900, 2190, 2238, 2391, 2401, 2956
TseFI GTSAC 5 cut(s) 62, 217, 806, 1764, 2710
TseI GCWGC 4 cut(s) 646, 1525, 2262, 3206
Tsp45I GTSAC 5 cut(s) 62, 217, 806, 1764, 2710
TspGWI ACGGA 2 cut(s) 1813, 3293
TspMI CCCGGG 1 cut(s) 2558
TspRI CASTG 7 cut(s) 67, 1900, 2190, 2238, 2391, 2401, 2956
Van91I CCANNNNNTGG 1 cut(s) 1608
Vha464I CTTAAG 3 cut(s) 1271, 3161, 3221
VneI GTGCAC 2 cut(s) 2186, 3156
VpaK11BI GGWCC 1 cut(s) 1069
XapI RAATTY 7 cut(s) 1022, 2150, 2323, 2356, 2803, 2849, 3371
XbaI TCTAGA 1 cut(s) 671
XceI RCATGY 5 cut(s) 336, 805, 2337, 2778, 3189
XhoI CTCGAG 1 cut(s) 1961
XmaI CCCGGG 1 cut(s) 2558
XmiI GTMKAC 2 cut(s) 1182, 2963
XmnI GAANNNNTTC 1 cut(s) 2324
XspI CTAG 8 cut(s) 369, 672, 975, 1775, 2070, 2249, 2378, 2645
ZrmI AGTACT 3 cut(s) 2344, 2572, 2859
Zsp2I ATGCAT 4 cut(s) 529, 1578, 2308, 3443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.