Prupe.3G201700_v2.0.a1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0003045
Physical Location & Seq
Forward (+)
21003466 .. 21013504
10039 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G201700.3

Sequence Viewer

Length: 3738 bp
ATGTATCTGAATTTCAGGGTCTCTGGCTTTAATGGCATTTTACCTGGTAATTTTAAGTTGAATAAGGCAACGGAGCCTTTGCATGGGCCTAATTGTGTCAGAAAATGGAGAAGGAAGCTTCTACTTCTCGGACTTTTAGGTTTTATTACAATTATTTGGTTTTTCGTGGGTTTCAATGATGGAACTTTGGGGATGAGAGAGAAGACTCCAGACATGTCTGAAGGGAAGGCTCGGATCCTGCAGCAGCATTTCAATGTCAGCAAGGACCAGCTTCTTGCTTTAGCTTCCTTATTCTCCGAATCAGATCAGATAGCATCCCTTGAATGTACCAAAGAACCAGGACCTGGAATGACGTTAAGTGATGACATTACCTGTGCTCTGAAGGTGCTATGTTCAGATAGTCAGGAGTTTCAGAAGCGTCATAAATGGGTCACAGAAAATGTAGAAGCCAGGGACCAATGCCTGGTTCAAGATGAGAACATCCCCAGGGAGCTTCACCTGTCATTGCTGGAGGACAAATCCGTATCATGCACTCCGCAGTCTACAATTTCAGCTAATAGGATTTGTGAAAAGAAAAATTTCGGATCAGGGGTTCCGGTGGAGTGTGCAAAAGATGATTCTCAGATGCGTTGCGTCATGGTAATACGATATTGGTGGGCTTTTGTTGGATTGATCTTGATCTACAAAATGTCTGGTTTTTCTTTGAAATTGTGGAGGAACCAAAAACAAAAGCTCGTTCATGAGTGGCCATTTACTTGGCAACTTAAAATGGTTCAAGAGCAGCCATTGGCTCGGAGGGTGCAGCCAGAGAAACAGCAACAGCAAGCCCAAAGTCCTCCTAAGGTTGCTGGAAAGTGGAGGAAGAATCTACTAATAGTATTTTTCGTATTTGGAGTCATTACATCATTTTGGTTATTTTGGCACTTGAATGAAAGAGATTTTTTGTGGAGAGAAGAAACTCTTGCCAACATGTGTGATGAACGAGCACGGATGTTGCAGGATCAATTTAATGTGAGCTTGAATCATGTTCATGCTTTGGCTATTCTTGTCTCCACCTTTCACCATGGGAAACATCCCTCAGCAATTGATCAGAAAACATTTGGTGAATACACAGAGAGAACAGCTTTTGAGAGACCCCTTACTAGTGGTGTTGCTTATGCTTTAAAAGTTACTCATGCTGAGAGGGAGCAATTTGAGAAGGAACATGGATGGACAATAAAAAAAATGGAAACTGAGGACCAGACTCTAGTCCAAGATTTTCTTCCAGAAAGCTTGGCCCCTGCCCCTATTCAAGATGAATATGCACCTGTGATATTTTCTCAAGAAACTGTCTCCCATATTGTCTCTATTGACATGATGTCAGGAAAGGAAGACCGTGAGAACATCTTGCGAGCAAGGGCAACTGCAAAGGGAGTACTGACTTCCCCCTTTAAGCTATTGAAATCCAATCACCTGGGAGTTGTACTCACGTTCGCTGTCTATGATACTGATTTGCCTCCAGATGCCACGTCAGAGCATCGTAGTCAAGCTACTGTGGGGTATCTAGGTGCATCTTATGATGTACCTTCACTGGTGGAGAAGCTCCTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATGCATCTGCTCCGGTCAACATGTATGGTACTGATGTTATTGACACTGGCCTGTTGCACATTAGCAATCTTGATTTTGGTGATCCACAACGCAAGCATGAAATGCACTGCAGGTTTAAGCAAAAACGTCCGTTTCCTTTGACAGCAGTAAGTGCATCAATGGGAGTCCTAGTCATTACCTTGCTAGTTGGTCATATTTTTCATGCGGCCATAAGCCGAATAGCAAAAGTGGAGGCAGACTATTGTGCGATGATGGAACTCAAAGTTCGTGCAGAAGCTGCAGATGTGGCCAAATCCCAGTTCCTTGCAACTGTTTCCCATGAGATCAGGACACCAATGAATGGTGTTTTAGGCATGCTCCAAATGCTGATGGACACTGATCTAAAACCGAACCAACAGGATTATGCTGAGACTGCTCATGCTAGTGGGAGAGAGCTGATATCACTGATAAATGAGGTTCTTGATCAGGCCAAAATCGAATCAGGCAGGCTGGAACTTGAAACCGTACCTTTTGATCTGCGTTCAGTGCTTGATAATGTTCTATCGCTTTTGTCTGGGAAATCTAATGAGAAAGGGATTGAGTTGGCTGTGTATGTCTCCAATATGGTACCTGAGGTTGTCATCGGGGATCCTGGACGCCTTCGGCAGATAATTACAAATCTTGTTGGGAATTCAATTAAGTTCACCCATGACAAAGGACACATATTTGTCTCGGTGCATCTGGCAGATGAAGTGAGGGCTCCACCGGATGTTATGGACGAAGTTTTAAGACAAGGCTTGAATTTAGTTGGAGACATCTCAAACAAAACTTATAATACATTGAGTGGCTTTCCCGTTGTTGATAGATGGAAAAGCTGGGAATGTTTTAAGGCATTAAGCAGCACAACAATGGAGGAACCTGATATGATCAAATTACTAGTAACAGTTGAGGATACTGGTGTAGGAATTCCTCTTGATGCACAAAGTCGCATTTTCACACCTTTTATGCAGGCTGATAGCTCAACTTCTAGAACTTATGGCGGTACTGGAATAGGATTGAGCATTAGCAAACGTCTGGTAGATCTCATGAGTGGGGAGATAGGTTTTGTGAGTGAACCTGGCACTGGCAGTACCTTTTCATTTACTGGATCTTTTGGAAAGGGAGAAAAAAGTTCTCTAGAGACAAAGTGGCAACAGTATGAACCAGCTGTCTCAGAATTTGAAGGGCTGAGAGCATTGGTAATAGATAAAAGTATCATCCGAACTGAGGTCACAAAATACCATGTGCAGAGGTTGGGGATATTTGTAGATACAGCTTCCAGTCTGGAATCAGCATGCTCTTATCTATCTAATTCTGGCAAGACAAGTCTCTCCTCACAGTTGACCATGGTTCTTATCGACAAAGATGTTTGGGATAAAGAAACTGGTCTTACATTCAGTCAGTCGCTTAAAGAGCACAGACAAAGCAACAACGTGGAATCCCTCATAAACCTTCCGAAGATATTTCTCTTGGCTACATCCATTACACCTACTGAATGCAAGGAACTAACATCTGCTGGTCTTGTAGATGAAGTATTGATCAAGCCTCTTCGGTTAAGTGTCATAATTGCTTGCTTCCAAGATGCCCTTGGAAGTCGTAAGAAGAGGCTTCTAAATCTTAAAAAACCTACTCTGGGAAAGTTACTGAGAGAGAAGAAAATTTTGGTGGTGGATGACAATGCAGTTAACAGAAGAGTGGCTGAAGGTGCTTTAAAGAAGTATGGAGCAATTGTCACCTGTGTGGACAGCGGAAAGGCTGCTTTATTAATGCTTAAGCCACCACACAATTTTGATGCTTGCTTTATGGATCTCCAAATGCCAGAAATGGATGGGTTTGAAGCAACTCGGCTGATCCGTGGTATGGAGAGTGAGGTAAAAGAGAAAATTGCATCCAAGGAACCATCAATTGAGATGTTTGGCAATGTCCAAACTTGGCACACGCCAATATTGGCTATGACGGCTGATGTGATTCAGGCTTCAAATGAAGAGTGCATGAAGTGTGGGATGGATGACTATGTGTCAAAGCCATTCGAAGAAGAACAGTTATACTCAGCAGTGGCACGTTTCTTTGAATCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1246

Amino Acids

139.46

Weight (kDa)

6.13

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2451
Acc36I ACCTGC 1 cut(s) 1740
Acc65I GGTACC 1 cut(s) 2245
AccB1I GGYRCC 1 cut(s) 2245
AccB7I CCANNNNNTGG 3 cut(s) 344, 463, 1979
AccI GTMKAC 1 cut(s) 542
AciI CCGC 4 cut(s) 536, 1844, 2658, 3405
AcoI YGGCCR 3 cut(s) 746, 1845, 1926
AcsI RAATTY 7 cut(s) 10, 577, 2308, 2419, 2583, 2834, 3315
AcuI CTGAAG 3 cut(s) 240, 401, 3378
AcyI GRCGYC 1 cut(s) 2275
AfaI GTAC 9 cut(s) 328, 1416, 1464, 1563, 1669, 2145, 2247, 2662, 2749
AfiI CCNNNNNNNGG 8 cut(s) 83, 344, 463, 1979, 2741, 2884, 3290, 3517
AflII CTTAAG 1 cut(s) 3428
AflIII ACRYGT 3 cut(s) 213, 969, 1659
AhdI GACNNNNNGTC 2 cut(s) 1357, 3673
AhlI ACTAGT 2 cut(s) 1142, 2554
AjiI CACGTC 1 cut(s) 1509
AjnI CCWGG 9 cut(s) 43, 337, 343, 449, 462, 485, 1452, 2269, 2734
AjuI GAANNNNNNNTTGG 8 cut(s) 450, 482, 1245, 1277, 1922, 1954, 3302, 3334
AleI CACNNNNGTG 1 cut(s) 3395
AloI GAACNNNNNNTCC 2 cut(s) 576, 608
Alw21I GWGCWC 3 cut(s) 379, 988, 3075
AlwNI CAGNNNCTG 2 cut(s) 344, 1916
AoxI GGCC 7 cut(s) 86, 746, 1275, 1687, 1845, 1926, 2106
ApeKI GCWGC 7 cut(s) 241, 244, 781, 802, 1916, 2517, 3413
ApoI RAATTY 7 cut(s) 10, 577, 2308, 2419, 2583, 2834, 3315
AseI ATTAAT 1 cut(s) 3422
Asp700I GAANNNNTTC 1 cut(s) 578
Asp718I GGTACC 1 cut(s) 2245
AspS9I GGNCC 6 cut(s) 86, 265, 341, 454, 1237, 1276
AsuHPI GGTGA 7 cut(s) 488, 1052, 1115, 1442, 1730, 2314, 3382
AsuII TTCGAA 1 cut(s) 3687
AvaII GGWCC 4 cut(s) 265, 341, 454, 1237
AxyI CCTNAGG 2 cut(s) 840, 2250
BaeI ACNNNNGTAYC 4 cut(s) 1659, 1692, 2562, 2595
BalI TGGCCA 2 cut(s) 748, 1928
BamHI GGATCC 2 cut(s) 234, 2266
BanI GGYRCC 1 cut(s) 2245
BanII GRGCYC 1 cut(s) 2380
BbsI GAAGAC 2 cut(s) 209, 1377
Bbv12I GWGCWC 3 cut(s) 379, 988, 3075
BbvCI CCTCAGC 1 cut(s) 1078
BbvI GCAGC 7 cut(s) 253, 256, 793, 814, 1903, 2529, 3400
BccI CCATC 8 cut(s) 173, 1203, 1885, 2002, 2478, 3479, 3565, 3655
BceAI ACGGC 1 cut(s) 3630
BciT130I CCWGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
BciVI GTATCC 1 cut(s) 2563
BclI TGATCA 4 cut(s) 1087, 2101, 2544, 3195
BcuI ACTAGT 2 cut(s) 1142, 2554
BfaI CTAG 9 cut(s) 1143, 1247, 1544, 1808, 1823, 2061, 2555, 2646, 2795
BfmI CTRYAG 3 cut(s) 239, 1747, 1917
BfrI CTTAAG 1 cut(s) 3428
BfuAI ACCTGC 1 cut(s) 1740
BfuI GTATCC 1 cut(s) 2563
BglII AGATCT 1 cut(s) 2698
BisI GCNGC 8 cut(s) 242, 245, 782, 803, 1845, 1917, 2518, 3414
BlsI GCNGC 8 cut(s) 243, 246, 783, 804, 1846, 1918, 2519, 3415
BmcAI AGTACT 1 cut(s) 1416
Bme1390I CCNGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
Bme18I GGWCC 4 cut(s) 265, 341, 454, 1237
BmeRI GACNNNNNGTC 2 cut(s) 1357, 3673
BmgBI CACGTC 1 cut(s) 1509
BmgT120I GGNCC 6 cut(s) 86, 265, 341, 454, 1237, 1276
BmrFI CCNGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
BmrI ACTGGG 1 cut(s) 1930
BmuI ACTGGG 1 cut(s) 1930
BoxI GACNNNNGTC 1 cut(s) 1247
BpiI GAAGAC 2 cut(s) 209, 1377
BplI GAGNNNNNCTC 2 cut(s) 1449, 1481
BpmI CTGGAG 3 cut(s) 192, 530, 1482
Bpu10I CCTNAGC 1 cut(s) 1078
Bpu14I TTCGAA 1 cut(s) 3687
BpuEI CTTGAG 1 cut(s) 1305
BsaBI GATNNNNATC 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 2275
BsaI GGTCTC 2 cut(s) 25, 1126
BsaJI CCNNGG 9 cut(s) 450, 485, 486, 1063, 1453, 3003, 3244, 3511, 3549
BsaWI WCCGGW 4 cut(s) 595, 1651, 2383, 3731
BsaXI ACNNNNNCTCC 4 cut(s) 1794, 1824, 3372, 3402
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 344, 463, 1979, 2741, 2884, 3290, 3517
Bse1I ACTGG 9 cut(s) 1575, 1690, 1936, 2578, 2668, 2746, 2767, 2937, 3046
Bse21I CCTNAGG 2 cut(s) 840, 2250
Bse3DI GCAATG 2 cut(s) 503, 3583
Bse8I GATNNNNATC 1 cut(s) 677
BseBI CCWGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
BseDI CCNNGG 9 cut(s) 450, 485, 486, 1063, 1453, 3003, 3244, 3511, 3549
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 8 cut(s) 83, 344, 463, 1979, 2741, 2884, 3290, 3517
BseMI GCAATG 2 cut(s) 503, 3583
BseNI ACTGG 9 cut(s) 1575, 1690, 1936, 2578, 2668, 2746, 2767, 2937, 3046
BseRI GAGGAG 1 cut(s) 2980
BseXI GCAGC 7 cut(s) 253, 256, 793, 814, 1903, 2529, 3400
BseYI CCCAGC 1 cut(s) 2493
BsgI GTGCAG 4 cut(s) 821, 1571, 1929, 2924
BshFI GGCC 7 cut(s) 88, 748, 1277, 1689, 1847, 1928, 2108
BshNI GGYRCC 1 cut(s) 2245
BsiHKAI GWGCWC 3 cut(s) 379, 988, 3075
BsiSI CCGG 4 cut(s) 596, 1652, 2384, 3732
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 8 cut(s) 83, 344, 463, 1979, 2741, 2884, 3290, 3517
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 3158
BsnI GGCC 7 cut(s) 88, 748, 1277, 1689, 1847, 1928, 2108
Bso31I GGTCTC 2 cut(s) 25, 1126
Bsp119I TTCGAA 1 cut(s) 3687
Bsp1286I GDGCHC 4 cut(s) 379, 988, 2380, 3075
Bsp19I CCATGG 2 cut(s) 1063, 3003
BspACI CCGC 4 cut(s) 536, 1844, 2658, 3405
BspANI GGCC 7 cut(s) 88, 748, 1277, 1689, 1847, 1928, 2108
BspHI TCATGA 2 cut(s) 739, 2703
BspMAI CTGCAG 3 cut(s) 243, 1751, 1921
BspMI ACCTGC 1 cut(s) 1740
BspT104I TTCGAA 1 cut(s) 3687
BspT107I GGYRCC 1 cut(s) 2245
BspTI CTTAAG 1 cut(s) 3428
BspTNI GGTCTC 2 cut(s) 25, 1126
BsrDI GCAATG 2 cut(s) 503, 3583
BsrI ACTGG 9 cut(s) 1575, 1690, 1936, 2578, 2668, 2746, 2767, 2937, 3046
BssECI CCNNGG 9 cut(s) 450, 485, 486, 1063, 1453, 3003, 3244, 3511, 3549
BssNI GRCGYC 1 cut(s) 2275
BssT1I CCWWGG 4 cut(s) 1063, 3003, 3244, 3549
Bst2UI CCWGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
Bst4CI ACNGT 9 cut(s) 1330, 1376, 1534, 1951, 2143, 2563, 2814, 2997, 3699
Bst6I CTCTTC 4 cut(s) 3210, 3254, 3343, 3636
BstACI GRCGYC 1 cut(s) 2275
BstAFI CTTAAG 1 cut(s) 3428
BstAPI GCANNNNNTGC 3 cut(s) 1741, 1790, 1916
BstBI TTCGAA 1 cut(s) 3687
BstDSI CCRYGG 3 cut(s) 1063, 3003, 3511
BstNI CCWGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
BstNSI RCATGY 5 cut(s) 217, 973, 1663, 1996, 2955
BstPAI GACNNNNGTC 1 cut(s) 1247
BstSCI CCNGG 9 cut(s) 43, 337, 343, 449, 462, 485, 1452, 2269, 2734
BstSFI CTRYAG 3 cut(s) 239, 1747, 1917
BstV1I GCAGC 7 cut(s) 253, 256, 793, 814, 1903, 2529, 3400
BstV2I GAAGAC 2 cut(s) 209, 1377
BstX2I RGATCY 5 cut(s) 234, 2266, 2698, 2765, 3463
BstXI CCANNNNNNTGG 2 cut(s) 756, 1453
BstYI RGATCY 5 cut(s) 234, 2266, 2698, 2765, 3463
Bsu36I CCTNAGG 2 cut(s) 840, 2250
BsuI GTATCC 1 cut(s) 2563
BsuRI GGCC 7 cut(s) 88, 748, 1277, 1689, 1847, 1928, 2108
BtgI CCRYGG 3 cut(s) 1063, 3003, 3511
BtgZI GCGATG 1 cut(s) 1901
BtrI CACGTC 1 cut(s) 1509
BtsI GCAGTG 2 cut(s) 1744, 3717
BtsIMutI CAGTG 8 cut(s) 1568, 1683, 1744, 2013, 2081, 2169, 2739, 3717
BveI ACCTGC 1 cut(s) 1740
CaiI CAGNNNCTG 2 cut(s) 344, 1916
CciI TCATGA 2 cut(s) 739, 2703
Cfr13I GGNCC 6 cut(s) 86, 265, 341, 454, 1237, 1276
CseI GACGC 3 cut(s) 407, 622, 2283
CsiI ACCWGGT 1 cut(s) 43
Csp6I GTAC 9 cut(s) 327, 1415, 1463, 1562, 1668, 2144, 2246, 2661, 2748
CviQI GTAC 9 cut(s) 327, 1415, 1463, 1562, 1668, 2144, 2246, 2661, 2748
DraI TTTAAA 2 cut(s) 1164, 3369
DriI GACNNNNNGTC 2 cut(s) 1357, 3673
EaeI YGGCCR 3 cut(s) 746, 1845, 1926
Eam1104I CTCTTC 4 cut(s) 3210, 3254, 3343, 3636
Eam1105I GACNNNNNGTC 2 cut(s) 1357, 3673
EarI CTCTTC 4 cut(s) 3210, 3254, 3343, 3636
Eco130I CCWWGG 4 cut(s) 1063, 3003, 3244, 3549
Eco24I GRGCYC 1 cut(s) 2380
Eco31I GGTCTC 2 cut(s) 25, 1126
Eco32I GATATC 1 cut(s) 2079
Eco47I GGWCC 4 cut(s) 265, 341, 454, 1237
Eco57I CTGAAG 3 cut(s) 240, 401, 3378
Eco81I CCTNAGG 2 cut(s) 840, 2250
EcoO109I RGGNCCY 1 cut(s) 341
EcoRI GAATTC 2 cut(s) 2308, 2583
EcoRII CCWGG 9 cut(s) 43, 337, 343, 449, 462, 485, 1452, 2269, 2734
EcoRV GATATC 1 cut(s) 2079
EcoT14I CCWWGG 4 cut(s) 1063, 3003, 3244, 3549
EcoT22I ATGCAT 1 cut(s) 1645
EcoT38I GRGCYC 1 cut(s) 2380
ErhI CCWWGG 4 cut(s) 1063, 3003, 3244, 3549
FalI AAGNNNNNCTT 6 cut(s) 945, 977, 1245, 1277, 3228, 3260
FaqI GGGAC 1 cut(s) 467
FbaI TGATCA 4 cut(s) 1087, 2101, 2544, 3195
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 8 cut(s) 242, 245, 782, 803, 1845, 1917, 2518, 3414
FriOI GRGCYC 1 cut(s) 2380
Fsp4HI GCNGC 8 cut(s) 242, 245, 782, 803, 1845, 1917, 2518, 3414
FspBI CTAG 9 cut(s) 1143, 1247, 1544, 1808, 1823, 2061, 2555, 2646, 2795
GluI GCNGC 8 cut(s) 242, 245, 782, 803, 1845, 1917, 2518, 3414
GsaI CCCAGC 1 cut(s) 2497
GsuI CTGGAG 3 cut(s) 192, 530, 1482
HaeIII GGCC 7 cut(s) 88, 748, 1277, 1689, 1847, 1928, 2108
HapII CCGG 4 cut(s) 596, 1652, 2384, 3732
HgaI GACGC 3 cut(s) 407, 622, 2283
Hin1I GRCGYC 1 cut(s) 2275
HincII GTYRAC 3 cut(s) 1657, 3000, 3343
HindII GTYRAC 3 cut(s) 1657, 3000, 3343
HindIII AAGCTT 2 cut(s) 116, 1270
HpaI GTTAAC 1 cut(s) 3343
HpaII CCGG 4 cut(s) 596, 1652, 2384, 3732
HphI GGTGA 7 cut(s) 488, 1052, 1115, 1442, 1730, 2314, 3382
Hpy166II GTNNAC 7 cut(s) 543, 1657, 2322, 2732, 3000, 3343, 3400
Hpy8I GTNNAC 7 cut(s) 543, 1657, 2322, 2732, 3000, 3343, 3400
HpyCH4III ACNGT 9 cut(s) 1330, 1376, 1534, 1951, 2143, 2563, 2814, 2997, 3699
HpyCH4IV ACGT 7 cut(s) 353, 1469, 1508, 1765, 2689, 3090, 3718
HpySE526I ACGT 7 cut(s) 353, 1469, 1508, 1765, 2689, 3090, 3718
Hsp92I GRCGYC 1 cut(s) 2275
KpnI GGTACC 1 cut(s) 2249
Ksp22I TGATCA 4 cut(s) 1087, 2101, 2544, 3195
KspAI GTTAAC 1 cut(s) 3343
LmnI GCTCC 8 cut(s) 73, 490, 1186, 1587, 1654, 2001, 2383, 3380
Lsp1109I GCAGC 7 cut(s) 253, 256, 793, 814, 1903, 2529, 3400
MabI ACCWGGT 1 cut(s) 43
MaeI CTAG 9 cut(s) 1143, 1247, 1544, 1808, 1823, 2061, 2555, 2646, 2795
MaeII ACGT 7 cut(s) 353, 1469, 1508, 1765, 2689, 3090, 3718
MaeIII GTNAC 6 cut(s) 430, 1168, 2557, 2887, 3297, 3388
MfeI CAATTG 4 cut(s) 1083, 1611, 3384, 3561
MflI RGATCY 5 cut(s) 234, 2266, 2698, 2765, 3463
MhlI GDGCHC 4 cut(s) 379, 988, 2380, 3075
MlsI TGGCCA 2 cut(s) 748, 1928
MluNI TGGCCA 2 cut(s) 748, 1928
MlyI GAGTC 4 cut(s) 199, 903, 1237, 1812
MmeI TCCRAC 2 cut(s) 646, 2407
Mox20I TGGCCA 2 cut(s) 748, 1928
Mph1103I ATGCAT 1 cut(s) 1645
MroXI GAANNNNTTC 1 cut(s) 578
MscI TGGCCA 2 cut(s) 748, 1928
MslI CAYNNNNRTG 6 cut(s) 252, 927, 1029, 2061, 2525, 3395
Msp20I TGGCCA 2 cut(s) 748, 1928
MspA1I CMGCKG 2 cut(s) 2825, 3405
MspCI CTTAAG 1 cut(s) 3428
MspI CCGG 4 cut(s) 596, 1652, 2384, 3732
MspR9I CCNGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
MunI CAATTG 4 cut(s) 1083, 1611, 3384, 3561
Mva1269I GAATGC 1 cut(s) 3158
MvaI CCWGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
NcoI CCATGG 2 cut(s) 1063, 3003
NmeAIII GCCGAG 1 cut(s) 3481
NmuCI GTSAC 3 cut(s) 430, 2887, 3388
NsiI ATGCAT 1 cut(s) 1645
NspI RCATGY 5 cut(s) 217, 973, 1663, 1996, 2955
NspV TTCGAA 1 cut(s) 3687
OliI CACNNNNGTG 1 cut(s) 3395
PaeI GCATGC 2 cut(s) 1996, 2955
PagI TCATGA 2 cut(s) 739, 2703
PasI CCCWGGG 1 cut(s) 486
PciI ACATGT 3 cut(s) 213, 969, 1659
PcsI WCGNNNNNNNCGW 1 cut(s) 3508
PctI GAATGC 1 cut(s) 3158
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 9 cut(s) 299, 617, 865, 1021, 2117, 2945, 3095, 3625, 3728
PflMI CCANNNNNTGG 3 cut(s) 344, 463, 1979
PfoI TCCNGGA 1 cut(s) 2269
PkrI GCNGC 8 cut(s) 243, 246, 783, 804, 1846, 1918, 2519, 3415
PleI GAGTC 4 cut(s) 199, 902, 1237, 1811
PpsI GAGTC 4 cut(s) 199, 902, 1237, 1811
PpuMI RGGWCCY 1 cut(s) 341
PscI ACATGT 3 cut(s) 213, 969, 1659
PshAI GACNNNNGTC 1 cut(s) 1247
PshBI ATTAAT 1 cut(s) 3422
PsiI TTATAA 1 cut(s) 2451
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 9 cut(s) 43, 337, 343, 449, 462, 485, 1452, 2269, 2734
PspFI CCCAGC 1 cut(s) 2493
PspGI CCWGG 9 cut(s) 43, 337, 343, 449, 462, 485, 1452, 2269, 2734
PspPI GGNCC 6 cut(s) 86, 265, 341, 454, 1237, 1276
PspPPI RGGWCCY 1 cut(s) 341
PstI CTGCAG 3 cut(s) 243, 1751, 1921
PstNI CAGNNNCTG 2 cut(s) 344, 1916
PsuI RGATCY 5 cut(s) 234, 2266, 2698, 2765, 3463
PvuII CAGCTG 1 cut(s) 2825
RsaI GTAC 9 cut(s) 328, 1416, 1464, 1563, 1669, 2145, 2247, 2662, 2749
RsaNI GTAC 9 cut(s) 327, 1415, 1463, 1562, 1668, 2144, 2246, 2661, 2748
RseI CAYNNNNRTG 6 cut(s) 252, 927, 1029, 2061, 2525, 3395
SatI GCNGC 8 cut(s) 242, 245, 782, 803, 1845, 1917, 2518, 3414
Sau96I GGNCC 6 cut(s) 86, 265, 341, 454, 1237, 1276
ScaI AGTACT 1 cut(s) 1416
SchI GAGTC 4 cut(s) 199, 903, 1237, 1812
ScrFI CCNGG 9 cut(s) 45, 339, 345, 451, 464, 487, 1454, 2271, 2736
SduI GDGCHC 4 cut(s) 379, 988, 2380, 3075
SexAI ACCWGGT 1 cut(s) 43
SfcI CTRYAG 3 cut(s) 239, 1747, 1917
SfuI TTCGAA 1 cut(s) 3687
SinI GGWCC 4 cut(s) 265, 341, 454, 1237
SmiMI CAYNNNNRTG 6 cut(s) 252, 927, 1029, 2061, 2525, 3395
SmlI CTYRAG 2 cut(s) 1320, 3428
SmoI CTYRAG 2 cut(s) 1320, 3428
SpeI ACTAGT 2 cut(s) 1142, 2554
SphI GCATGC 2 cut(s) 1996, 2955
SsiI CCGC 4 cut(s) 536, 1844, 2658, 3405
SspI AATATT 1 cut(s) 3603
SspMI CTAG 9 cut(s) 1143, 1247, 1544, 1808, 1823, 2061, 2555, 2646, 2795
StyD4I CCNGG 9 cut(s) 43, 337, 343, 449, 462, 485, 1452, 2269, 2734
StyI CCWWGG 4 cut(s) 1063, 3003, 3244, 3549
TaaI ACNGT 9 cut(s) 1330, 1376, 1534, 1951, 2143, 2563, 2814, 2997, 3699
TaiI ACGT 7 cut(s) 356, 1472, 1511, 1768, 2692, 3093, 3721
TaqI TCGA 3 cut(s) 2115, 3015, 3687
TatI WGTACW 2 cut(s) 1414, 1462
TauI GCSGC 1 cut(s) 1847
TfiI GAWTC 9 cut(s) 299, 617, 865, 1021, 2117, 2945, 3095, 3625, 3728
TscAI CASTG 8 cut(s) 1575, 1690, 1751, 2020, 2088, 2169, 2746, 3717
TseFI GTSAC 3 cut(s) 430, 2887, 3388
TseI GCWGC 7 cut(s) 241, 244, 781, 802, 1916, 2517, 3413
Tsp45I GTSAC 3 cut(s) 430, 2887, 3388
TspGWI ACGGA 5 cut(s) 86, 511, 1003, 1758, 3500
TspRI CASTG 8 cut(s) 1575, 1690, 1751, 2020, 2088, 2169, 2746, 3717
Van91I CCANNNNNTGG 3 cut(s) 344, 463, 1979
Vha464I CTTAAG 1 cut(s) 3428
VpaK11BI GGWCC 4 cut(s) 265, 341, 454, 1237
VspI ATTAAT 1 cut(s) 3422
XapI RAATTY 7 cut(s) 10, 577, 2308, 2419, 2583, 2834, 3315
XbaI TCTAGA 2 cut(s) 2645, 2794
XceI RCATGY 5 cut(s) 217, 973, 1663, 1996, 2955
XcmI CCANNNNNNNNNTGG 1 cut(s) 3242
XmiI GTMKAC 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 9 cut(s) 1143, 1247, 1544, 1808, 1823, 2061, 2555, 2646, 2795
ZrmI AGTACT 1 cut(s) 1416
Zsp2I ATGCAT 1 cut(s) 1645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.