Rw2G043030

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003045
Physical Location & Seq
Forward (+)
69405638 .. 69415842
10205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043030.1

Sequence Viewer

Length: 3099 bp
ATGTTGTTTGATAAAGGGTTGTTCGTGGGTTTTTGTTGGAATGATTTTGTTCTACCAATTGTCGGGTTTTTCTTTGAAATATGGAGGAACCAGAAGCAGAAGCTGGTTCATGAGTGCCCATGCCAACAGAAATTGGTTCAAGAGCAGCCATTGGGTCGTAAGAAGCAGCCAGAGAAACAGCAACAACAAGTCCACAGTCCTCCTAAGGTTGCTTGCACGTGGAGGAAGAAACTCCTAATCGTATTTGTCTTATTTGGAGTTATTACGTCAATTTGGTTATTTTTCCACTTGAATGAAAGAGATTTTGTGTGGAGAGAAGAAACACTTTCCAACATGTGTGATGAACGAGCCCGGATGTTGCAAGATCAGTTCAATGTGAGCCTGAACCATGTTCATGCTTTAGCTATTCTCGTCTCCACCTTTCACCATGGAAAGCATCCTTCTGCCATTGATCAGAAAACATTTGGTGAATATACAGAGAGAACAGCTTTTGAGAGACCACTTACTAGTGGTGTTGCTTATGCTTTAAAAGTTACTCATGCTGAGAGGGAACAATTCGAGAAGGAACATGGATGGACAATAAAGAAAATGGAAACTGAGGACCAGACTCTTGTCCAAGATTTTCTTCCAGAAAGCTTGGATCCTGCCCCTATTCAAGATGAATATGCGCCTGTGATATTTTCTCAAGAAACTGTCTCCCATATTGTCTCGATTGACATGATGTCGGGAAAGGATGACCGCGAGAACATCTTGCGAGCAAGGTCAACAGGAAAGGGAGTACTGACTTCCCCTTTTAAGCTATTGAAATCCAATCACTTGGGAGTTGTTCTTACATTTGCTGTGTATAATATGGACTTGCCTCCAGATGCCACATCAGAGCAACGAGTTCAAGCGACTGTGGGGTATCTAGGTGCATCTTACGATGTCCCATCACTGGTGGAGAAGCTCCTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATGCATCTTCTCTGATCAACATGTATGGAACTGATGTTATTGATACTGGGCTGTTGCACATTAGCAGCCTTGATTTTGGTGATCCACAACGCAGACATGAAATGCACTGCAGGTTTAAGCATAAACCTCCTTTTCCTTGGACAGCAGTAAGTGCGGCAGTGGGAGTCCTAGTCATTACCTTGCTAGTTGGTCATATTTTTCATGCGGCCATAAGCCGAATAGCAAAGGTAGAGGCAGACTATTGTGTGATGGAACAACTCAAAGTTCGTGCAGAAGCTGCAGATGTGGCCAAATCACAGTTCCTTGCAACTGTTTCCCATGAAATCCGGACACCAATGAATGGTGTTTTAGGTATGCTGCAAATGCTGATGGACACAAATCTTGATGCCAACCAACAGGATTATGCTGAGACTGCGCATGCTAGTGGAAGAGATCTCATATCGCTGATAAATGAGGTTCTTGATCAGGCCAAAATAGAATCAGGCCGGCTGGAACTTGAAACTGTACCTTTTGATCTTCGTTCGGTGCTTGATAATGTTCTATCACTTTTCTCAGGAAAAACTAATGAGAAAGGGATTGAGTTGGCTGTCTATGTCTCCAATATGGTCCCTGAAGTTGTCATCGGAGACCCAGGACGCCTTCGGCAGATTATTACAAACCTTGTTGGGAATTCAATCAAGTTCACCCATGACAAAGGACATATATTTGTCTCGGTGCATCTGGCGGATGAAGTGAAGGGTCCACCTGATTTTATGGATGAAGTTCTAAGACAAGGCTTGAATTTAGTTGGAGACCTCTCAAACAAAACTTATACATTGAGTGGCTTTCCTGTGGTTGATAGATGGAAAAGTTGGGAACGTTTTAAAGCATTAAGCAGCACAACAATGGAGGAACCTGATATGATAAAATTAATTGTAACAGTTGAGGATACAGGTGTAGGAATTCCACTAGATGCACAAAGTCGCATCTTCACGCCTTTTATGCAGGCGGATAGTTCCACTTCTAGAACTTATGGTGGTACTGGAATAGGATTGAGCATTAGCAAACGTTTGGTCGATCTCATGTGTGGGGAGATAGGGTTTGTGAGTGAACCTGGCATTGGCAGTACCTTTTCATTTACTGGGTCGTTTCTGAGAGGGAAGACAAGTTCTCTAGATATGAAGTGGCCTCAGTATGAACCAGCTGTCTCAGAATTTCGAGGACTGAGAGCACTGGTAATAGATAAGAGAATCACCCGAACTGAAGTCACAAGATATCATATGCAGAGGTTGGGAATATCGGCGGATATTGCTTCCAGTCTTGAATCAGCATGCTCTTATCTATCTAGTACGAGCAAGACAAGCCTATCCACATGTTTGGCCATGGTTCTTATCGACAAAGATGTTTGGGATAAAGAAACTGGTGTTACGTTCCATCAGTCAGTTAAAGAGCACAGGCAAAACAGCAGCGTGGAGTCTCCCGTGAACTTTCCAAAGATATTTCTCCTGGCTACCTCCATTAGCTCTACTGAACGTAATGAACTAAAATCTTCTGGTCTTGTAGATAATGTATTGATGAAGCCTCTTCGGTTAAGTGTCTTAATTGCCTGCTTCCAAGAGGCCCTTGTAAGTGGTAAAACGAGGCTTGTGAACATAAAAAAACCAACTCTTGGAAAGTTACTACGAGGCAGGAAGATTTTGGTAGTGGATGATAATGCAGTCAACAGAAGAGTCGCAGAAGGTGCTTTAAAGAAATATGGAGCAATTGTCACCTGTGTTGATAGTGGGAAGACTGCTTTAGACATGCTTAAGCCACCACACAACTTTGACGCTTGCTTTATGGACCTCCAAATGCCAGAAATGGACGGATTCGAAGCAACTCGTCGAATTCGCTGTCTGGAGAGTGAGGTTAATGAGAAAATTGCATCTGGGGAAGCACCAATCGAGATGTTTGGCAATGTGGAGAATTGGCACACACCAATATTAGCTATGACGGCTGATGTGATTCAGGCTTCAAATGAAGAATGCATGAATTGTGGGATGGATGGCTATGTGTCAAAGCCATTTGAAGAAGAACAATTATATACAGCAGTAGCTAAATTCTTCGAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1032

Amino Acids

115.39

Weight (kDa)

5.94

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 149 - 343 8.4e-35 CHASE domain
HisKA PF00512 431 - 496 7.4e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 637 - 708 2.1e-17 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 731 - 869 1.5e-37 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 893 - 1025 1.1e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1419
Acc36I ACCTGC 1 cut(s) 1104
AccB7I CCANNNNNTGG 2 cut(s) 1343, 2651
AccII CGCG 1 cut(s) 741
AccIII TCCGGA 1 cut(s) 1329
AciI CCGC 6 cut(s) 739, 1157, 1208, 1727, 1991, 2285
AclI AACGTT 2 cut(s) 1861, 2050
AclWI GGATC 3 cut(s) 635, 648, 1079
AcoI YGGCCR 3 cut(s) 1209, 1290, 2361
AcsI RAATTY 6 cut(s) 1672, 1783, 1944, 2195, 2868, 3080
AcuI CTGAAG 2 cut(s) 1635, 2265
AcvI CACGTG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 1639
AfaI GTAC 5 cut(s) 780, 1509, 2023, 2110, 2332
AfiI CCNNNNNNNGG 5 cut(s) 62, 934, 1343, 2102, 2651
AflII CTTAAG 1 cut(s) 2789
AflIII ACRYGT 3 cut(s) 333, 1023, 2354
AhdI GACNNNNNGTC 1 cut(s) 721
AhlI ACTAGT 1 cut(s) 506
AjnI CCWGG 3 cut(s) 1633, 2095, 2487
AjuI GAANNNNNNNTTGG 6 cut(s) 609, 641, 1286, 1318, 2085, 2117
AloI GAACNNNNNNTCC 2 cut(s) 2134, 2166
Alw21I GWGCWC 2 cut(s) 2215, 2436
AlwI GGATC 3 cut(s) 635, 648, 1079
AlwNI CAGNNNCTG 2 cut(s) 103, 1280
Aor13HI TCCGGA 1 cut(s) 1329
AoxI GGCC 7 cut(s) 1209, 1290, 1470, 1486, 2168, 2361, 2601
ApeKI GCWGC 7 cut(s) 145, 166, 1068, 1280, 1360, 1878, 2448
ApoI RAATTY 6 cut(s) 1672, 1783, 1944, 2195, 2868, 3080
ArsI GACNNNNNNTTYG 4 cut(s) 2040, 2072, 2860, 2892
AseI ATTAAT 1 cut(s) 1913
AspLEI GCGC 2 cut(s) 670, 1420
AspS9I GGNCC 5 cut(s) 601, 1609, 1742, 2602, 2824
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 6 cut(s) 416, 479, 1094, 1678, 2227, 2743
AsuII TTCGAA 1 cut(s) 2853
AvaII GGWCC 4 cut(s) 601, 1609, 1742, 2824
AxyI CCTNAGG 1 cut(s) 204
BaeGI GKGCMC 1 cut(s) 119
BaeI ACNNNNGTAYC 2 cut(s) 1923, 1956
BalI TGGCCA 2 cut(s) 1292, 2363
BamHI GGATCC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 352
BbrPI CACGTG 1 cut(s) 219
BbsI GAAGAC 2 cut(s) 2150, 2777
Bbv12I GWGCWC 2 cut(s) 2215, 2436
BbvI GCAGC 7 cut(s) 157, 178, 1080, 1267, 1347, 1890, 2460
BccI CCATC 8 cut(s) 567, 937, 1246, 1366, 1839, 2424, 3016, 3020
BceAI ACGGC 1 cut(s) 2991
BciT130I CCWGG 3 cut(s) 1635, 2097, 2489
BciVI GTATCC 1 cut(s) 1924
BclI TGATCA 3 cut(s) 451, 1017, 1465
BcnI CCSGG 1 cut(s) 352
BcuI ACTAGT 1 cut(s) 506
BfaI CTAG 9 cut(s) 507, 908, 1172, 1187, 1425, 1952, 2007, 2156, 2328
BfmI CTRYAG 2 cut(s) 1111, 1281
BfrI CTTAAG 1 cut(s) 2789
BfuAI ACCTGC 1 cut(s) 1104
BfuI GTATCC 1 cut(s) 1924
BglII AGATCT 1 cut(s) 1435
BisI GCNGC 9 cut(s) 146, 167, 1069, 1158, 1209, 1281, 1361, 1879, 2449
BlsI GCNGC 9 cut(s) 147, 168, 1070, 1159, 1210, 1282, 1362, 1880, 2450
BmcAI AGTACT 1 cut(s) 780
Bme1390I CCNGG 4 cut(s) 352, 1635, 2097, 2489
Bme18I GGWCC 4 cut(s) 601, 1609, 1742, 2824
BmeRI GACNNNNNGTC 1 cut(s) 721
BmgT120I GGNCC 5 cut(s) 601, 1609, 1742, 2602, 2824
BmiI GGNNCC 5 cut(s) 89, 642, 1611, 1743, 1896
BmrFI CCNGG 4 cut(s) 352, 1635, 2097, 2489
BmrI ACTGGG 2 cut(s) 1059, 2133
BmsI GCATC 9 cut(s) 445, 856, 923, 1016, 1378, 1729, 1945, 1977, 2915
BmuI ACTGGG 2 cut(s) 1059, 2133
BoxI GACNNNNGTC 1 cut(s) 611
BpiI GAAGAC 2 cut(s) 2150, 2777
BpmI CTGGAG 2 cut(s) 846, 2900
Bpu14I TTCGAA 1 cut(s) 2853
BpuEI CTTGAG 1 cut(s) 669
BpuMI CCSGG 1 cut(s) 352
BsaAI YACGTR 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 1639
BsaI GGTCTC 3 cut(s) 490, 1623, 1788
BsaJI CCNNGG 4 cut(s) 427, 1139, 1633, 2364
BsaWI WCCGGW 1 cut(s) 1329
BsaXI ACNNNNNCTCC 6 cut(s) 1158, 1188, 2066, 2096, 2733, 2763
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 934, 1343, 2102, 2651
Bse118I RCCGGY 1 cut(s) 1488
Bse1I ACTGG 7 cut(s) 939, 1054, 2029, 2128, 2220, 2298, 2407
Bse21I CCTNAGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 2944
BseAI TCCGGA 1 cut(s) 1329
BseBI CCWGG 3 cut(s) 1635, 2097, 2489
BseDI CCNNGG 4 cut(s) 427, 1139, 1633, 2364
BseGI GGATG 9 cut(s) 360, 436, 578, 739, 1735, 1765, 2695, 3027, 3031
BseLI CCNNNNNNNGG 5 cut(s) 62, 934, 1343, 2102, 2651
BseMI GCAATG 1 cut(s) 2944
BseMII CTCAG 8 cut(s) 534, 588, 1401, 1569, 2126, 2186, 2198, 2205
BseNI ACTGG 7 cut(s) 939, 1054, 2029, 2128, 2220, 2298, 2407
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 7 cut(s) 157, 178, 1080, 1267, 1347, 1890, 2460
BsgI GTGCAG 2 cut(s) 935, 1293
Bsh1236I CGCG 1 cut(s) 741
BshFI GGCC 7 cut(s) 1211, 1292, 1472, 1488, 2170, 2363, 2603
BsiHKAI GWGCWC 2 cut(s) 2215, 2436
BsiSI CCGG 3 cut(s) 352, 1330, 1489
BslFI GGGAC 2 cut(s) 911, 1595
BslI CCNNNNNNNGG 5 cut(s) 62, 934, 1343, 2102, 2651
BsmBI CGTCTC 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 911, 1595
BsmI GAATGC 1 cut(s) 3011
BsnI GGCC 7 cut(s) 1211, 1292, 1472, 1488, 2170, 2363, 2603
Bso31I GGTCTC 3 cut(s) 490, 1623, 1788
Bsp119I TTCGAA 1 cut(s) 2853
Bsp1286I GDGCHC 4 cut(s) 119, 352, 2215, 2436
Bsp13I TCCGGA 1 cut(s) 1329
Bsp143I GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
Bsp19I CCATGG 2 cut(s) 427, 2364
BspACI CCGC 6 cut(s) 739, 1157, 1208, 1727, 1991, 2285
BspANI GGCC 7 cut(s) 1211, 1292, 1472, 1488, 2170, 2363, 2603
BspCNI CTCAG 8 cut(s) 535, 589, 1402, 1568, 2127, 2185, 2199, 2204
BspEI TCCGGA 1 cut(s) 1329
BspFNI CGCG 1 cut(s) 741
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 89, 642, 1611, 1743, 1896
BspMAI CTGCAG 2 cut(s) 1115, 1285
BspMI ACCTGC 1 cut(s) 1104
BspPI GGATC 3 cut(s) 635, 648, 1079
BspT104I TTCGAA 1 cut(s) 2853
BspTI CTTAAG 1 cut(s) 2789
BspTNI GGTCTC 3 cut(s) 490, 1623, 1788
BsrDI GCAATG 1 cut(s) 2944
BsrFI RCCGGY 1 cut(s) 1488
BsrI ACTGG 7 cut(s) 939, 1054, 2029, 2128, 2220, 2298, 2407
BssAI RCCGGY 1 cut(s) 1488
BssECI CCNNGG 4 cut(s) 427, 1139, 1633, 2364
BssMI GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
BssNI GRCGYC 1 cut(s) 1639
BssT1I CCWWGG 3 cut(s) 427, 1139, 2364
Bst2UI CCWGG 3 cut(s) 1635, 2097, 2489
Bst4CI ACNGT 7 cut(s) 197, 694, 898, 1302, 1315, 1507, 1924
Bst6I CTCTTC 3 cut(s) 1426, 2571, 2704
BstACI GRCGYC 1 cut(s) 1639
BstAFI CTTAAG 1 cut(s) 2789
BstAPI GCANNNNNTGC 3 cut(s) 1154, 1280, 2723
BstBAI YACGTR 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 2853
BstDSI CCRYGG 2 cut(s) 427, 2364
BstF5I GGATG 9 cut(s) 360, 436, 578, 739, 1735, 1765, 2695, 3027, 3031
BstFNI CGCG 1 cut(s) 741
BstHHI GCGC 2 cut(s) 670, 1420
BstKTI GATC 9 cut(s) 367, 454, 643, 1020, 1087, 1438, 1468, 1519, 2062
BstMBI GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
BstNI CCWGG 3 cut(s) 1635, 2097, 2489
BstNSI RCATGY 6 cut(s) 337, 1027, 1424, 2316, 2358, 2788
BstPAI GACNNNNGTC 1 cut(s) 611
BstSCI CCNGG 4 cut(s) 350, 1633, 2095, 2487
BstSFI CTRYAG 2 cut(s) 1111, 1281
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 1 cut(s) 741
BstV1I GCAGC 7 cut(s) 157, 178, 1080, 1267, 1347, 1890, 2460
BstV2I GAAGAC 2 cut(s) 2150, 2777
BstX2I RGATCY 2 cut(s) 640, 1435
BstXI CCANNNNNNTGG 2 cut(s) 817, 2359
BstYI RGATCY 2 cut(s) 640, 1435
Bsu36I CCTNAGG 1 cut(s) 204
BsuI GTATCC 1 cut(s) 1924
BsuRI GGCC 7 cut(s) 1211, 1292, 1472, 1488, 2170, 2363, 2603
BtgI CCRYGG 2 cut(s) 427, 2364
BtsCI GGATG 9 cut(s) 360, 436, 578, 739, 1735, 1765, 2695, 3027, 3031
BtsI GCAGTG 2 cut(s) 1108, 1167
BtsIMutI CAGTG 4 cut(s) 932, 1108, 1167, 2213
BveI ACCTGC 1 cut(s) 1104
CaiI CAGNNNCTG 2 cut(s) 103, 1280
CciI TCATGA 1 cut(s) 109
CfoI GCGC 2 cut(s) 670, 1420
Cfr10I RCCGGY 1 cut(s) 1488
Cfr13I GGNCC 5 cut(s) 601, 1609, 1742, 2602, 2824
CseI GACGC 2 cut(s) 1647, 2819
Csp6I GTAC 5 cut(s) 779, 1508, 2022, 2109, 2331
CviQI GTAC 5 cut(s) 779, 1508, 2022, 2109, 2331
DpnI GATC 9 cut(s) 366, 453, 642, 1019, 1086, 1437, 1467, 1518, 2061
DpnII GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
DraI TTTAAA 3 cut(s) 528, 1867, 2730
DriI GACNNNNNGTC 1 cut(s) 721
EaeI YGGCCR 3 cut(s) 1209, 1290, 2361
Eam1104I CTCTTC 3 cut(s) 1426, 2571, 2704
Eam1105I GACNNNNNGTC 1 cut(s) 721
EarI CTCTTC 3 cut(s) 1426, 2571, 2704
EciI GGCGGA 3 cut(s) 1742, 2006, 2300
Eco130I CCWWGG 3 cut(s) 427, 1139, 2364
Eco24I GRGCYC 1 cut(s) 352
Eco31I GGTCTC 3 cut(s) 490, 1623, 1788
Eco32I GATATC 1 cut(s) 2258
Eco47I GGWCC 4 cut(s) 601, 1609, 1742, 2824
Eco57I CTGAAG 2 cut(s) 1635, 2265
Eco72I CACGTG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 2602
EcoRI GAATTC 3 cut(s) 1672, 1944, 2868
EcoRII CCWGG 3 cut(s) 1633, 2095, 2487
EcoRV GATATC 1 cut(s) 2258
EcoT14I CCWWGG 3 cut(s) 427, 1139, 2364
EcoT22I ATGCAT 2 cut(s) 1009, 3011
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 3 cut(s) 427, 1139, 2364
Esp3I CGTCTC 1 cut(s) 418
FalI AAGNNNNNCTT 6 cut(s) 309, 341, 609, 641, 2589, 2621
FaqI GGGAC 2 cut(s) 911, 1595
FauNDI CATATG 1 cut(s) 2262
FbaI TGATCA 3 cut(s) 451, 1017, 1465
Fnu4HI GCNGC 9 cut(s) 146, 167, 1069, 1158, 1209, 1281, 1361, 1879, 2449
FokI GGATG 9 cut(s) 367, 423, 585, 746, 1742, 1772, 2702, 3034, 3038
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 9 cut(s) 146, 167, 1069, 1158, 1209, 1281, 1361, 1879, 2449
FspBI CTAG 9 cut(s) 507, 908, 1172, 1187, 1425, 1952, 2007, 2156, 2328
FspI TGCGCA 1 cut(s) 1419
GlaI GCGC 2 cut(s) 669, 1419
GluI GCNGC 9 cut(s) 146, 167, 1069, 1158, 1209, 1281, 1361, 1879, 2449
GsuI CTGGAG 2 cut(s) 846, 2900
HaeIII GGCC 7 cut(s) 1211, 1292, 1472, 1488, 2170, 2363, 2603
HapII CCGG 3 cut(s) 352, 1330, 1489
HgaI GACGC 2 cut(s) 1647, 2819
HhaI GCGC 2 cut(s) 670, 1420
Hin1I GRCGYC 1 cut(s) 1639
Hin6I GCGC 2 cut(s) 668, 1418
HinP1I GCGC 2 cut(s) 668, 1418
HincII GTYRAC 2 cut(s) 765, 2704
HindII GTYRAC 2 cut(s) 765, 2704
HindIII AAGCTT 1 cut(s) 634
HpaII CCGG 3 cut(s) 352, 1330, 1489
HphI GGTGA 6 cut(s) 416, 479, 1094, 1678, 2227, 2743
Hpy166II GTNNAC 8 cut(s) 193, 765, 1686, 1745, 2093, 2467, 2632, 2704
Hpy188I TCNGA 6 cut(s) 456, 877, 1017, 1628, 2136, 2194
Hpy8I GTNNAC 8 cut(s) 193, 765, 1686, 1745, 2093, 2467, 2632, 2704
Hpy99I CGWCG 1 cut(s) 2868
HpyAV CCTTC 5 cut(s) 450, 556, 1652, 1732, 2714
HpyCH4III ACNGT 7 cut(s) 197, 694, 898, 1302, 1315, 1507, 1924
HpyCH4IV ACGT 6 cut(s) 218, 266, 1861, 2050, 2411, 2515
HpySE526I ACGT 6 cut(s) 218, 266, 1861, 2050, 2411, 2515
Hsp92I GRCGYC 1 cut(s) 1639
HspAI GCGC 2 cut(s) 668, 1418
Kpn2I TCCGGA 1 cut(s) 1329
KroI GCCGGC 1 cut(s) 1488
KroNI GCCGGC 1 cut(s) 1490
Ksp22I TGATCA 3 cut(s) 451, 1017, 1465
Kzo9I GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
LmnI GCTCC 2 cut(s) 951, 2741
Lsp1109I GCAGC 7 cut(s) 157, 178, 1080, 1267, 1347, 1890, 2460
LweI GCATC 9 cut(s) 445, 856, 923, 1016, 1378, 1729, 1945, 1977, 2915
MaeI CTAG 9 cut(s) 507, 908, 1172, 1187, 1425, 1952, 2007, 2156, 2328
MaeII ACGT 6 cut(s) 218, 266, 1861, 2050, 2411, 2515
MaeIII GTNAC 6 cut(s) 532, 1918, 2248, 2407, 2658, 2749
MalI GATC 9 cut(s) 366, 453, 642, 1019, 1086, 1437, 1467, 1518, 2061
MboI GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
MfeI CAATTG 3 cut(s) 57, 975, 2745
MflI RGATCY 2 cut(s) 640, 1435
MhlI GDGCHC 4 cut(s) 119, 352, 2215, 2436
MlsI TGGCCA 2 cut(s) 1292, 2363
MluNI TGGCCA 2 cut(s) 1292, 2363
MlyI GAGTC 5 cut(s) 601, 1176, 2465, 2721, 3098
MmeI TCCRAC 3 cut(s) 17, 354, 1771
Mox20I TGGCCA 2 cut(s) 1292, 2363
Mph1103I ATGCAT 2 cut(s) 1009, 3011
MroI TCCGGA 1 cut(s) 1329
MroNI GCCGGC 1 cut(s) 1488
MscI TGGCCA 2 cut(s) 1292, 2363
MslI CAYNNNNRTG 4 cut(s) 291, 393, 1425, 1886
Msp20I TGGCCA 2 cut(s) 1292, 2363
MspA1I CMGCKG 1 cut(s) 2186
MspCI CTTAAG 1 cut(s) 2789
MspI CCGG 3 cut(s) 352, 1330, 1489
MspR9I CCNGG 4 cut(s) 352, 1635, 2097, 2489
MunI CAATTG 3 cut(s) 57, 975, 2745
Mva1269I GAATGC 1 cut(s) 3011
MvaI CCWGG 3 cut(s) 1635, 2097, 2489
MvnI CGCG 1 cut(s) 741
NaeI GCCGGC 1 cut(s) 1490
NciI CCSGG 1 cut(s) 352
NcoI CCATGG 2 cut(s) 427, 2364
NdeI CATATG 1 cut(s) 2262
NdeII GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
NgoMIV GCCGGC 1 cut(s) 1488
NlaIV GGNNCC 5 cut(s) 89, 642, 1611, 1743, 1896
NmuCI GTSAC 2 cut(s) 2248, 2749
NsbI TGCGCA 1 cut(s) 1419
NsiI ATGCAT 2 cut(s) 1009, 3011
NspI RCATGY 6 cut(s) 337, 1027, 1424, 2316, 2358, 2788
NspV TTCGAA 1 cut(s) 2853
PaeI GCATGC 2 cut(s) 1424, 2316
PagI TCATGA 1 cut(s) 109
PciI ACATGT 3 cut(s) 333, 1023, 2354
PctI GAATGC 1 cut(s) 3011
PdiI GCCGGC 1 cut(s) 1490
PfeI GAWTC 5 cut(s) 1481, 2232, 2306, 2850, 2986
PflMI CCANNNNNTGG 2 cut(s) 1343, 2651
PkrI GCNGC 9 cut(s) 147, 168, 1070, 1159, 1210, 1282, 1362, 1880, 2450
PleI GAGTC 5 cut(s) 601, 1175, 2464, 2720, 3097
PmaCI CACGTG 1 cut(s) 219
PmlI CACGTG 1 cut(s) 219
PpsI GAGTC 5 cut(s) 601, 1175, 2464, 2720, 3097
Ppu21I YACGTR 1 cut(s) 219
PscI ACATGT 3 cut(s) 333, 1023, 2354
PshAI GACNNNNGTC 1 cut(s) 611
PshBI ATTAAT 1 cut(s) 1913
Psp1406I AACGTT 2 cut(s) 1861, 2050
Psp6I CCWGG 3 cut(s) 1633, 2095, 2487
PspCI CACGTG 1 cut(s) 219
PspGI CCWGG 3 cut(s) 1633, 2095, 2487
PspN4I GGNNCC 5 cut(s) 89, 642, 1611, 1743, 1896
PspPI GGNCC 5 cut(s) 601, 1609, 1742, 2602, 2824
PstI CTGCAG 2 cut(s) 1115, 1285
PstNI CAGNNNCTG 2 cut(s) 103, 1280
PsuI RGATCY 2 cut(s) 640, 1435
PvuII CAGCTG 1 cut(s) 2186
RsaI GTAC 5 cut(s) 780, 1509, 2023, 2110, 2332
RsaNI GTAC 5 cut(s) 779, 1508, 2022, 2109, 2331
RseI CAYNNNNRTG 4 cut(s) 291, 393, 1425, 1886
SatI GCNGC 9 cut(s) 146, 167, 1069, 1158, 1209, 1281, 1361, 1879, 2449
Sau3AI GATC 9 cut(s) 364, 451, 640, 1017, 1084, 1435, 1465, 1516, 2059
Sau96I GGNCC 5 cut(s) 601, 1609, 1742, 2602, 2824
ScaI AGTACT 1 cut(s) 780
SchI GAGTC 5 cut(s) 601, 1176, 2465, 2721, 3098
ScrFI CCNGG 4 cut(s) 352, 1635, 2097, 2489
SduI GDGCHC 4 cut(s) 119, 352, 2215, 2436
SfaNI GCATC 9 cut(s) 445, 856, 923, 1016, 1378, 1729, 1945, 1977, 2915
SfcI CTRYAG 2 cut(s) 1111, 1281
SfuI TTCGAA 1 cut(s) 2853
SinI GGWCC 4 cut(s) 601, 1609, 1742, 2824
SmiMI CAYNNNNRTG 4 cut(s) 291, 393, 1425, 1886
SmlI CTYRAG 2 cut(s) 684, 2789
SmoI CTYRAG 2 cut(s) 684, 2789
SpeI ACTAGT 1 cut(s) 506
SphI GCATGC 2 cut(s) 1424, 2316
SsiI CCGC 6 cut(s) 739, 1157, 1208, 1727, 1991, 2285
SspI AATATT 1 cut(s) 2964
SspMI CTAG 9 cut(s) 507, 908, 1172, 1187, 1425, 1952, 2007, 2156, 2328
StyD4I CCNGG 4 cut(s) 350, 1633, 2095, 2487
StyI CCWWGG 3 cut(s) 427, 1139, 2364
TaaI ACNGT 7 cut(s) 197, 694, 898, 1302, 1315, 1507, 1924
TaiI ACGT 6 cut(s) 221, 269, 1864, 2053, 2414, 2518
TaqI TCGA 9 cut(s) 558, 710, 2058, 2200, 2376, 2853, 2866, 2925, 3087
TatI WGTACW 1 cut(s) 778
TauI GCSGC 2 cut(s) 1160, 1211
TfiI GAWTC 5 cut(s) 1481, 2232, 2306, 2850, 2986
TscAI CASTG 4 cut(s) 939, 1115, 1167, 2220
TseFI GTSAC 2 cut(s) 2248, 2749
TseI GCWGC 7 cut(s) 145, 166, 1068, 1280, 1360, 1878, 2448
Tsp45I GTSAC 2 cut(s) 2248, 2749
TspGWI ACGGA 1 cut(s) 2862
TspRI CASTG 4 cut(s) 939, 1115, 1167, 2220
Van91I CCANNNNNTGG 2 cut(s) 1343, 2651
Vha464I CTTAAG 1 cut(s) 2789
VpaK11BI GGWCC 4 cut(s) 601, 1609, 1742, 2824
VspI ATTAAT 1 cut(s) 1913
XapI RAATTY 6 cut(s) 1672, 1783, 1944, 2195, 2868, 3080
XbaI TCTAGA 2 cut(s) 2006, 2155
XceI RCATGY 6 cut(s) 337, 1027, 1424, 2316, 2358, 2788
XspI CTAG 9 cut(s) 507, 908, 1172, 1187, 1425, 1952, 2007, 2156, 2328
ZrmI AGTACT 1 cut(s) 780
Zsp2I ATGCAT 2 cut(s) 1009, 3011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.