RchiOBHm_Chr2g0156741

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003045
Physical Location & Seq
Forward (+)
73306994 .. 73316750
9757 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52551

Sequence Viewer

Length: 3729 bp
ATGTATCTGAATTGCAGGGTGTCTGGTTTTAATGGCAGACTACCTGCTAATTTCAAGTTAAACAAGGCAATGGAGCCCTTGGATGGGCCAAATTGTGTCAGTTATTCGAGAAGAAAGCTTCTACTTCTTGGGGTTTTAGGTTTTATTACAGTAGTGTGGTTTTTCCTGGGTTTCAATGAGGGACTTCTGGGGATGAAAGAGAAGACTCCAGAGATGTGTGGAGAGAAATCTCGAATCTTGCAGCAGCATTTCAATGTCAGCAAGAACCAGCTTCTGGCTTTAGCTTCCTTGTTCTCCGAATCAGATCAGATAGCATCCCTTGAATGTAACAAAGAAACAGGACATGGGATGCTTTTAACTGATGGCATTTCCTGTGCTTTGAAGGCAGTATGTTCAGATGAGAAAGAATTTCAAAAGCATCATAAATGGGTGGCAGAATATGTTGAAGCCAAGGACCAATGCCTGGTTCAAGATGAAAACATCCCCAGGAGGCTTGACCTATCAGTGCTACAGGAAAAGTCCTTGGCACACATTCCGCAGTCTACAGTATCTGCTAATAGAATTTGTCAAAAGGACAATTTGGGATCAGGGGCAAAGGTAGAGTGCACAAAAGTGGATTCTCAGATTTTATGTTGTTTGATAAAGGGTTGTTCGTGGGTTTTTGTTGGAATGATTTTGTTCTACCAATTGTCTGGTTTTTCTTTGAAATTATGGAGGAACCAGAAGCAGAAGCTGGTTCATGAGTGCCCATGCCAACAGAAATTGGTTCAAGAGCAGCCATTGGGTCGTAAGAAGCAGCCAGAGAAACAGCAACAACAAGTCCACAGTCCTCCTAAGGTTGCTTGCACGTGGAGGAAGAAACTCCTAATCGTATTTGTCTTATTTGGAGTTATTACGTCAATTTGGTTATTTTTCCACTTGAATGAAAGAGATTTTGTGTGGAGAGAAGAAACACTTTCCAACATGTGTGATGAACGAGCCCGGATGTTGCAAGATCAGTTCAATGTGAGCCTGAACCATGTTCATGCTTTAGCTATTCTCGTCTCCACCTTTCACCATGGAAAGCATCCTTCTGCCATTGATCAGAAAACATTTGGTGAATATACAGAGAGAACAGCTTTTGAGAGACCACTTACTAGTGGTGTTGCTTATGCTTTAAAAGTTACTCATGCTGAGAGGGAACAATTCGAGAAGGAACATGGATGGACAATAAAGAAAATGGAAACTGAGGACCAGACTCTTGTCCAAGATTTTCTTCCAGAAAGCTTGGATCCTGCCCCTATTCAAGATGAATATGCGCCTGTGATATTTTCTCAAGAAACTGTCTCCCATATTGTCTCGATTGACATGATGTCGGGAAAGGATGACCGCGAGAACATCTTGCGAGCAAGGTCAACAGGAAAGGGAGTACTGACTTCCCCTTTTAAGCTATTGAAATCCAATCACTTGGGAGTTGTTCTTACATTTGCTGTGTATAATATGGACTTGCCTCCAGATGCCACATCAGAGCAACGAGTTCAAGCGACTGTGGGGTATCTAGGTGCATCTTACGATGTCCCATCACTGGTGGAGAAGCTCCTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATGCATCTTCTCTGATCAACATGTATGGAACTGATGTTATTGATACTGGGCTGTTGCACATTAGCAGCCTTGATTTTGGTGATCCACAACGCAGACATGAAATGCACTGCAGGTTTAAGCATAAACCTCCTTTTCCTTGGACAGCAGTAAGTGCGGCAGTGGGAGTCCTAGTCATTACCTTGCTAGTTGGTCATATTTTTCATGCGGCCATAAGCCGAATAGCAAAGGTAGAGGCAGACTATTGTGTGATGGAACAACTCAAAGTTCGTGCAGAAGCTGCAGATGTGGCCAAATCACAGTTCCTTGCAACTGTTTCCCATGAAATCCGGACACCAATGAATGGTGTTTTAGGTATGCTGCAAATGCTGATGGACACAAATCTTGATGCCAACCAACAGGATTATGCTGAGACTGCGCATGCTAGTGGAAGAGATCTCATATCGCTGATAAATGAGGTTCTTGATCAGGCCAAAATAGAATCAGGCCGGCTGGAACTTGAAACTGTACCTTTTGATCTTCGTTCGGTGCTTGATAATGTTCTATCACTTTTCTCAGGAAAAACTAATGAGAAAGGGATTGAGTTGGCTGTCTATGTCTCCAATATGGTCCCTGAAGTTGTCATCGGAGACCCAGGACGCCTTCGGCAGATTATTACAAACCTTGTTGGGAATTCAATCAAGTTCACCCATGACAAAGGACATATATTTGTCTCGGTGCATCTGGCGGATGAAGTGAAGGGTCCACCTGATTTTATGGATGAAGTTCTAAGACAAGGCTTGAATTTAGTTGGAGACCTCTCAAACAAAACTTATACATTGAGTGGCTTTCCTGTGGTTGATAGATGGAAAAGTTGGGAACGTTTTAAAGCATTAAGCAGCACAACAATGGAGGAACCTGATATGATAAAATTAATTGTAACAGTTGAGGATACAGGTGTAGGAATTCCACTAGATGCACAAAGTCGCATCTTCACGCCTTTTATGCAGGCGGATAGTTCCACTTCTAGAACTTATGGTGGTACTGGAATAGGATTGAGCATTAGCAAACGTTTGGTCGATCTCATGTGTGGGGAGATAGGGTTTGTGAGTGAACCTGGCATTGGCAGTACCTTTTCATTTACTGGGTCGTTTCTGAGAGGGAAGACAAGTTCTCTAGATATGAAGTGGCCTCAGTATGAACCAGCTGTCTCAGAATTTCGAGGACTGAGAGCACTGGTAATAGATAAGAGAATCACCCGAACTGAAGTCACAAGATATCATATGCAGAGGTTGGGAATATCGGCGGATATTGCTTCCAGTCTTGAATCAGCATGCTCTTATCTATCTAGTACGAGCAAGACAAGCCTATCCACATGTTTGGCCATGGTTCTTATCGACAAAGATGTTTGGGATAAAGAAACTGGTGTTACGTTCCATCAGTCGGTTAAAGAGCACAGGCAAAACAGCAGCGTGGAGTCTCCCGTGAACTTTCCAAAGATATTTCTCCTGGCTACCTCCATTAGCTCTACTGAACGTAATGAACTAAAATCTTCTGGTCTTGTAGATAATGTATTGATGAAGCCTCTTCGGTTAAGTGTCTTAATTGCCTGCTTCCAAGAGGCCCTTGTAAGTGGTAAAACGAGGCTTGTGAACATAAAAAAACCAACTCTTGGAAAGTTACTACGAGGCAGGAAGATTTTGGTAGTGGATGATAATGCAGTCAACAGAAGAGTTGCAGAAGGTGCTTTAAAGAAATATGGAGCAATTGTCACCTGTGTTGATAGTGGGAAGACTGCTTTAGACATGCTTAAGCCACCACACAACTTTGACGCTTGCTTTATGGACCTCCAAATGCCAGAAATGGACGGATTCGAAGCAACTCGTCGAATTCGCTGTCTGGAGAGTGAGGTTAATGAGAAAATTGCATCTGGGGAAGCACCAATCGAGATGTTTGGCAATGTGGAGAATTGGCACACACCAATATTAGCTATGACGGCTGATGTGATTCAGGCTTCAAATGAAGAATGCATGAATTGTGGGATGGATGGCTATGTGTCAAAGCCATTTGAAGAAGAACAATTATATACAGCAGTAGCTAAATTCTTCGAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1242

Amino Acids

138.66

Weight (kDa)

6.27

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 359 - 553 1.1e-34 CHASE domain
HisKA PF00512 641 - 706 9.3e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 847 - 918 2.6e-17 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 941 - 1079 1.9e-37 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 1103 - 1235 1.4e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2049
Acc36I ACCTGC 2 cut(s) 52, 1734
AccB7I CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3281
AccI GTMKAC 1 cut(s) 542
AccII CGCG 1 cut(s) 1371
AccIII TCCGGA 1 cut(s) 1959
AciI CCGC 7 cut(s) 536, 1369, 1787, 1838, 2357, 2621, 2915
AclI AACGTT 2 cut(s) 2491, 2680
AclWI GGATC 4 cut(s) 592, 1265, 1278, 1709
AcoI YGGCCR 3 cut(s) 1839, 1920, 2991
AcsI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2574, 2825, 3498, 3710
AcuI CTGAAG 2 cut(s) 2265, 2895
AcvI CACGTG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 2269
AfaI GTAC 5 cut(s) 1410, 2139, 2653, 2740, 2962
AfiI CCNNNNNNNGG 9 cut(s) 83, 84, 274, 463, 1564, 1973, 2732, 3052, 3281
AflII CTTAAG 1 cut(s) 3419
AflIII ACRYGT 3 cut(s) 963, 1653, 2984
AhdI GACNNNNNGTC 1 cut(s) 1351
AhlI ACTAGT 1 cut(s) 1136
AjnI CCWGG 6 cut(s) 165, 462, 485, 2263, 2725, 3117
AjuI GAANNNNNNNTTGG 8 cut(s) 450, 482, 1239, 1271, 1916, 1948, 2715, 2747
AleI CACNNNNGTG 1 cut(s) 611
AloI GAACNNNNNNTCC 2 cut(s) 2764, 2796
Alw21I GWGCWC 3 cut(s) 608, 2845, 3066
Alw44I GTGCAC 1 cut(s) 604
AlwI GGATC 4 cut(s) 592, 1265, 1278, 1709
AlwNI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
Aor13HI TCCGGA 1 cut(s) 1959
AoxI GGCC 8 cut(s) 86, 1839, 1920, 2100, 2116, 2798, 2991, 3231
ApaLI GTGCAC 1 cut(s) 604
ApeKI GCWGC 9 cut(s) 241, 244, 775, 796, 1698, 1910, 1990, 2508, 3078
ApoI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2574, 2825, 3498, 3710
ArsI GACNNNNNNTTYG 4 cut(s) 2670, 2702, 3490, 3522
AseI ATTAAT 1 cut(s) 2543
AspLEI GCGC 2 cut(s) 1300, 2050
AspS9I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3232, 3454
AsuC2I CCSGG 1 cut(s) 982
AsuHPI GGTGA 6 cut(s) 1046, 1109, 1724, 2308, 2857, 3373
AsuII TTCGAA 1 cut(s) 3483
AvaII GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3454
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 2 cut(s) 608, 749
BaeI ACNNNNGTAYC 2 cut(s) 2553, 2586
BalI TGGCCA 2 cut(s) 1922, 2993
BamHI GGATCC 1 cut(s) 1270
BanII GRGCYC 2 cut(s) 78, 982
BbrPI CACGTG 1 cut(s) 849
BbsI GAAGAC 3 cut(s) 209, 2780, 3407
Bbv12I GWGCWC 3 cut(s) 608, 2845, 3066
BbvI GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2520, 3090
BceAI ACGGC 1 cut(s) 3621
BciT130I CCWGG 6 cut(s) 167, 464, 487, 2265, 2727, 3119
BciVI GTATCC 1 cut(s) 2554
BclI TGATCA 3 cut(s) 1081, 1647, 2095
BcnI CCSGG 1 cut(s) 982
BcuI ACTAGT 1 cut(s) 1136
BfaI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2582, 2637, 2786, 2958
BfmI CTRYAG 4 cut(s) 509, 543, 1741, 1911
BfrI CTTAAG 1 cut(s) 3419
BfuAI ACCTGC 2 cut(s) 52, 1734
BfuI GTATCC 1 cut(s) 2554
BglII AGATCT 1 cut(s) 2065
BmcAI AGTACT 1 cut(s) 1410
Bme1390I CCNGG 7 cut(s) 167, 464, 487, 982, 2265, 2727, 3119
Bme18I GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3454
BmeRI GACNNNNNGTC 1 cut(s) 1351
BmgT120I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3232, 3454
BmiI GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2526
BmrFI CCNGG 7 cut(s) 167, 464, 487, 982, 2265, 2727, 3119
BmrI ACTGGG 2 cut(s) 1689, 2763
BmuI ACTGGG 2 cut(s) 1689, 2763
BoxI GACNNNNGTC 1 cut(s) 1241
BpiI GAAGAC 3 cut(s) 209, 2780, 3407
BpmI CTGGAG 3 cut(s) 192, 1476, 3530
Bpu14I TTCGAA 1 cut(s) 3483
BpuEI CTTGAG 1 cut(s) 1299
BpuMI CCSGG 1 cut(s) 982
BsaAI YACGTR 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 2269
BsaI GGTCTC 3 cut(s) 1120, 2253, 2418
BsaJI CCNNGG 9 cut(s) 78, 166, 450, 485, 522, 1057, 1769, 2263, 2994
BsaWI WCCGGW 1 cut(s) 1959
BsaXI ACNNNNNCTCC 6 cut(s) 1788, 1818, 2696, 2726, 3363, 3393
Bsc4I CCNNNNNNNGG 9 cut(s) 83, 84, 274, 463, 1564, 1973, 2732, 3052, 3281
Bse118I RCCGGY 1 cut(s) 2118
Bse1I ACTGG 7 cut(s) 1569, 1684, 2659, 2758, 2850, 2928, 3037
Bse21I CCTNAGG 1 cut(s) 834
Bse3DI GCAATG 2 cut(s) 75, 3574
BseAI TCCGGA 1 cut(s) 1959
BseBI CCWGG 6 cut(s) 167, 464, 487, 2265, 2727, 3119
BseDI CCNNGG 9 cut(s) 78, 166, 450, 485, 522, 1057, 1769, 2263, 2994
BseLI CCNNNNNNNGG 9 cut(s) 83, 84, 274, 463, 1564, 1973, 2732, 3052, 3281
BseMI GCAATG 2 cut(s) 75, 3574
BseMII CTCAG 9 cut(s) 635, 1164, 1218, 2031, 2199, 2756, 2816, 2828, 2835
BseNI ACTGG 7 cut(s) 1569, 1684, 2659, 2758, 2850, 2928, 3037
BseSI GKGCMC 2 cut(s) 608, 749
BseXI GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2520, 3090
BsgI GTGCAG 2 cut(s) 1565, 1923
Bsh1236I CGCG 1 cut(s) 1371
BshFI GGCC 8 cut(s) 88, 1841, 1922, 2102, 2118, 2800, 2993, 3233
BsiHKAI GWGCWC 3 cut(s) 608, 2845, 3066
BsiSI CCGG 3 cut(s) 982, 1960, 2119
BslFI GGGAC 3 cut(s) 195, 1541, 2225
BslI CCNNNNNNNGG 9 cut(s) 83, 84, 274, 463, 1564, 1973, 2732, 3052, 3281
BsmBI CGTCTC 1 cut(s) 1048
BsmFI GGGAC 3 cut(s) 195, 1541, 2225
BsmI GAATGC 1 cut(s) 3641
BsnI GGCC 8 cut(s) 88, 1841, 1922, 2102, 2118, 2800, 2993, 3233
Bso31I GGTCTC 3 cut(s) 1120, 2253, 2418
Bsp119I TTCGAA 1 cut(s) 3483
Bsp1286I GDGCHC 6 cut(s) 78, 608, 749, 982, 2845, 3066
Bsp13I TCCGGA 1 cut(s) 1959
Bsp19I CCATGG 2 cut(s) 1057, 2994
BspACI CCGC 7 cut(s) 536, 1369, 1787, 1838, 2357, 2621, 2915
BspANI GGCC 8 cut(s) 88, 1841, 1922, 2102, 2118, 2800, 2993, 3233
BspCNI CTCAG 9 cut(s) 634, 1165, 1219, 2032, 2198, 2757, 2815, 2829, 2834
BspEI TCCGGA 1 cut(s) 1959
BspFNI CGCG 1 cut(s) 1371
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2526
BspMAI CTGCAG 2 cut(s) 1745, 1915
BspMI ACCTGC 2 cut(s) 52, 1734
BspPI GGATC 4 cut(s) 592, 1265, 1278, 1709
BspT104I TTCGAA 1 cut(s) 3483
BspTI CTTAAG 1 cut(s) 3419
BspTNI GGTCTC 3 cut(s) 1120, 2253, 2418
BsrDI GCAATG 2 cut(s) 75, 3574
BsrFI RCCGGY 1 cut(s) 2118
BsrI ACTGG 7 cut(s) 1569, 1684, 2659, 2758, 2850, 2928, 3037
BssAI RCCGGY 1 cut(s) 2118
BssECI CCNNGG 9 cut(s) 78, 166, 450, 485, 522, 1057, 1769, 2263, 2994
BssNI GRCGYC 1 cut(s) 2269
BssT1I CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2994
Bst2UI CCWGG 6 cut(s) 167, 464, 487, 2265, 2727, 3119
Bst4CI ACNGT 9 cut(s) 151, 547, 827, 1324, 1528, 1932, 1945, 2137, 2554
Bst6I CTCTTC 3 cut(s) 2056, 3201, 3334
BstACI GRCGYC 1 cut(s) 2269
BstAFI CTTAAG 1 cut(s) 3419
BstAPI GCANNNNNTGC 3 cut(s) 1784, 1910, 3353
BstBAI YACGTR 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 3483
BstDSI CCRYGG 2 cut(s) 1057, 2994
BstFNI CGCG 1 cut(s) 1371
BstHHI GCGC 2 cut(s) 1300, 2050
BstNI CCWGG 6 cut(s) 167, 464, 487, 2265, 2727, 3119
BstNSI RCATGY 6 cut(s) 967, 1657, 2054, 2946, 2988, 3418
BstPAI GACNNNNGTC 1 cut(s) 1241
BstSCI CCNGG 7 cut(s) 165, 462, 485, 980, 2263, 2725, 3117
BstSFI CTRYAG 4 cut(s) 509, 543, 1741, 1911
BstSLI GKGCMC 2 cut(s) 608, 749
BstUI CGCG 1 cut(s) 1371
BstV1I GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2520, 3090
BstV2I GAAGAC 3 cut(s) 209, 2780, 3407
BstX2I RGATCY 2 cut(s) 1270, 2065
BstXI CCANNNNNNTGG 3 cut(s) 692, 1447, 2989
BstYI RGATCY 2 cut(s) 1270, 2065
Bsu36I CCTNAGG 1 cut(s) 834
BsuI GTATCC 1 cut(s) 2554
BsuRI GGCC 8 cut(s) 88, 1841, 1922, 2102, 2118, 2800, 2993, 3233
BtgI CCRYGG 2 cut(s) 1057, 2994
BtsI GCAGTG 2 cut(s) 1738, 1797
BtsIMutI CAGTG 5 cut(s) 510, 1562, 1738, 1797, 2843
BveI ACCTGC 2 cut(s) 52, 1734
CaiI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
CciI TCATGA 1 cut(s) 739
CfoI GCGC 2 cut(s) 1300, 2050
Cfr10I RCCGGY 1 cut(s) 2118
Cfr13I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3232, 3454
CseI GACGC 2 cut(s) 2277, 3449
Csp6I GTAC 5 cut(s) 1409, 2138, 2652, 2739, 2961
CviQI GTAC 5 cut(s) 1409, 2138, 2652, 2739, 2961
DraI TTTAAA 3 cut(s) 1158, 2497, 3360
DriI GACNNNNNGTC 1 cut(s) 1351
EaeI YGGCCR 3 cut(s) 1839, 1920, 2991
Eam1104I CTCTTC 3 cut(s) 2056, 3201, 3334
Eam1105I GACNNNNNGTC 1 cut(s) 1351
EarI CTCTTC 3 cut(s) 2056, 3201, 3334
EciI GGCGGA 3 cut(s) 2372, 2636, 2930
Eco130I CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2994
Eco24I GRGCYC 2 cut(s) 78, 982
Eco31I GGTCTC 3 cut(s) 1120, 2253, 2418
Eco32I GATATC 1 cut(s) 2888
Eco47I GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3454
Eco57I CTGAAG 2 cut(s) 2265, 2895
Eco72I CACGTG 1 cut(s) 849
Eco81I CCTNAGG 1 cut(s) 834
EcoO109I RGGNCCY 1 cut(s) 3232
EcoRI GAATTC 3 cut(s) 2302, 2574, 3498
EcoRII CCWGG 6 cut(s) 165, 462, 485, 2263, 2725, 3117
EcoRV GATATC 1 cut(s) 2888
EcoT14I CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2994
EcoT22I ATGCAT 2 cut(s) 1639, 3641
EcoT38I GRGCYC 2 cut(s) 78, 982
ErhI CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2994
Esp3I CGTCTC 1 cut(s) 1048
FalI AAGNNNNNCTT 6 cut(s) 939, 971, 1239, 1271, 3219, 3251
FaqI GGGAC 3 cut(s) 195, 1541, 2225
FauNDI CATATG 1 cut(s) 2892
FbaI TGATCA 3 cut(s) 1081, 1647, 2095
FblI GTMKAC 1 cut(s) 542
FriOI GRGCYC 2 cut(s) 78, 982
FspBI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2582, 2637, 2786, 2958
FspI TGCGCA 1 cut(s) 2049
GlaI GCGC 2 cut(s) 1299, 2049
GsuI CTGGAG 3 cut(s) 192, 1476, 3530
HaeIII GGCC 8 cut(s) 88, 1841, 1922, 2102, 2118, 2800, 2993, 3233
HapII CCGG 3 cut(s) 982, 1960, 2119
HgaI GACGC 2 cut(s) 2277, 3449
HhaI GCGC 2 cut(s) 1300, 2050
Hin1I GRCGYC 1 cut(s) 2269
Hin6I GCGC 2 cut(s) 1298, 2048
HinP1I GCGC 2 cut(s) 1298, 2048
HincII GTYRAC 2 cut(s) 1395, 3334
HindII GTYRAC 2 cut(s) 1395, 3334
HindIII AAGCTT 2 cut(s) 116, 1264
HpaII CCGG 3 cut(s) 982, 1960, 2119
HphI GGTGA 6 cut(s) 1046, 1109, 1724, 2308, 2857, 3373
Hpy99I CGWCG 1 cut(s) 3498
HpyAV CCTTC 6 cut(s) 376, 1080, 1186, 2282, 2362, 3344
HpyCH4III ACNGT 9 cut(s) 151, 547, 827, 1324, 1528, 1932, 1945, 2137, 2554
HpyCH4IV ACGT 6 cut(s) 848, 896, 2491, 2680, 3041, 3145
HpySE526I ACGT 6 cut(s) 848, 896, 2491, 2680, 3041, 3145
Hsp92I GRCGYC 1 cut(s) 2269
HspAI GCGC 2 cut(s) 1298, 2048
Kpn2I TCCGGA 1 cut(s) 1959
KroI GCCGGC 1 cut(s) 2118
KroNI GCCGGC 1 cut(s) 2120
Ksp22I TGATCA 3 cut(s) 1081, 1647, 2095
LmnI GCTCC 3 cut(s) 73, 1581, 3371
Lsp1109I GCAGC 9 cut(s) 253, 256, 787, 808, 1710, 1897, 1977, 2520, 3090
MaeI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2582, 2637, 2786, 2958
MaeII ACGT 6 cut(s) 848, 896, 2491, 2680, 3041, 3145
MaeIII GTNAC 7 cut(s) 326, 1162, 2548, 2878, 3037, 3288, 3379
MfeI CAATTG 3 cut(s) 686, 1605, 3375
MflI RGATCY 2 cut(s) 1270, 2065
MhlI GDGCHC 6 cut(s) 78, 608, 749, 982, 2845, 3066
MlsI TGGCCA 2 cut(s) 1922, 2993
MluNI TGGCCA 2 cut(s) 1922, 2993
MlyI GAGTC 5 cut(s) 199, 1231, 1806, 3095, 3728
MmeI TCCRAC 3 cut(s) 646, 984, 2401
Mox20I TGGCCA 2 cut(s) 1922, 2993
Mph1103I ATGCAT 2 cut(s) 1639, 3641
MroI TCCGGA 1 cut(s) 1959
MroNI GCCGGC 1 cut(s) 2118
MscI TGGCCA 2 cut(s) 1922, 2993
MslI CAYNNNNRTG 6 cut(s) 252, 611, 921, 1023, 2055, 2516
Msp20I TGGCCA 2 cut(s) 1922, 2993
MspA1I CMGCKG 1 cut(s) 2816
MspCI CTTAAG 1 cut(s) 3419
MspI CCGG 3 cut(s) 982, 1960, 2119
MspR9I CCNGG 7 cut(s) 167, 464, 487, 982, 2265, 2727, 3119
MunI CAATTG 3 cut(s) 686, 1605, 3375
Mva1269I GAATGC 1 cut(s) 3641
MvaI CCWGG 6 cut(s) 167, 464, 487, 2265, 2727, 3119
MvnI CGCG 1 cut(s) 1371
NaeI GCCGGC 1 cut(s) 2120
NciI CCSGG 1 cut(s) 982
NcoI CCATGG 2 cut(s) 1057, 2994
NdeI CATATG 1 cut(s) 2892
NgoMIV GCCGGC 1 cut(s) 2118
NlaIV GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2526
NmuCI GTSAC 2 cut(s) 2878, 3379
NsbI TGCGCA 1 cut(s) 2049
NsiI ATGCAT 2 cut(s) 1639, 3641
NspI RCATGY 6 cut(s) 967, 1657, 2054, 2946, 2988, 3418
NspV TTCGAA 1 cut(s) 3483
OliI CACNNNNGTG 1 cut(s) 611
PaeI GCATGC 2 cut(s) 2054, 2946
PagI TCATGA 1 cut(s) 739
PciI ACATGT 3 cut(s) 963, 1653, 2984
PctI GAATGC 1 cut(s) 3641
PdiI GCCGGC 1 cut(s) 2120
PfeI GAWTC 8 cut(s) 234, 299, 617, 2111, 2862, 2936, 3480, 3616
PflMI CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3281
PleI GAGTC 5 cut(s) 199, 1231, 1805, 3094, 3727
PmaCI CACGTG 1 cut(s) 849
PmlI CACGTG 1 cut(s) 849
PpsI GAGTC 5 cut(s) 199, 1231, 1805, 3094, 3727
Ppu21I YACGTR 1 cut(s) 849
PscI ACATGT 3 cut(s) 963, 1653, 2984
PshAI GACNNNNGTC 1 cut(s) 1241
PshBI ATTAAT 1 cut(s) 2543
Psp1406I AACGTT 2 cut(s) 2491, 2680
Psp6I CCWGG 6 cut(s) 165, 462, 485, 2263, 2725, 3117
PspCI CACGTG 1 cut(s) 849
PspGI CCWGG 6 cut(s) 165, 462, 485, 2263, 2725, 3117
PspN4I GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2526
PspPI GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3232, 3454
PstI CTGCAG 2 cut(s) 1745, 1915
PstNI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
PsuI RGATCY 2 cut(s) 1270, 2065
PvuII CAGCTG 1 cut(s) 2816
RsaI GTAC 5 cut(s) 1410, 2139, 2653, 2740, 2962
RsaNI GTAC 5 cut(s) 1409, 2138, 2652, 2739, 2961
RseI CAYNNNNRTG 6 cut(s) 252, 611, 921, 1023, 2055, 2516
Sau96I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3232, 3454
ScaI AGTACT 1 cut(s) 1410
SchI GAGTC 5 cut(s) 199, 1231, 1806, 3095, 3728
ScrFI CCNGG 7 cut(s) 167, 464, 487, 982, 2265, 2727, 3119
SduI GDGCHC 6 cut(s) 78, 608, 749, 982, 2845, 3066
SfcI CTRYAG 4 cut(s) 509, 543, 1741, 1911
SfuI TTCGAA 1 cut(s) 3483
SinI GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3454
SmiMI CAYNNNNRTG 6 cut(s) 252, 611, 921, 1023, 2055, 2516
SmlI CTYRAG 2 cut(s) 1314, 3419
SmoI CTYRAG 2 cut(s) 1314, 3419
SpeI ACTAGT 1 cut(s) 1136
SphI GCATGC 2 cut(s) 2054, 2946
SsiI CCGC 7 cut(s) 536, 1369, 1787, 1838, 2357, 2621, 2915
SspI AATATT 1 cut(s) 3594
SspMI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2582, 2637, 2786, 2958
StyD4I CCNGG 7 cut(s) 165, 462, 485, 980, 2263, 2725, 3117
StyI CCWWGG 6 cut(s) 78, 450, 522, 1057, 1769, 2994
TaaI ACNGT 9 cut(s) 151, 547, 827, 1324, 1528, 1932, 1945, 2137, 2554
TaiI ACGT 6 cut(s) 851, 899, 2494, 2683, 3044, 3148
TatI WGTACW 1 cut(s) 1408
TauI GCSGC 2 cut(s) 1790, 1841
TfiI GAWTC 8 cut(s) 234, 299, 617, 2111, 2862, 2936, 3480, 3616
TscAI CASTG 5 cut(s) 510, 1569, 1745, 1797, 2850
TseFI GTSAC 2 cut(s) 2878, 3379
TseI GCWGC 9 cut(s) 241, 244, 775, 796, 1698, 1910, 1990, 2508, 3078
Tsp45I GTSAC 2 cut(s) 2878, 3379
TspGWI ACGGA 1 cut(s) 3492
TspRI CASTG 5 cut(s) 510, 1569, 1745, 1797, 2850
Van91I CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3281
Vha464I CTTAAG 1 cut(s) 3419
VneI GTGCAC 1 cut(s) 604
VpaK11BI GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3454
VspI ATTAAT 1 cut(s) 2543
XapI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2574, 2825, 3498, 3710
XbaI TCTAGA 2 cut(s) 2636, 2785
XceI RCATGY 6 cut(s) 967, 1657, 2054, 2946, 2988, 3418
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2582, 2637, 2786, 2958
ZrmI AGTACT 1 cut(s) 1410
Zsp2I ATGCAT 2 cut(s) 1639, 3641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.