MD17G1113000.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003045
Physical Location & Seq
Reverse (-)
9662143 .. 9670375
8233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1113000.v1.1.491

Sequence Viewer

Length: 3681 bp
ATGTATCTGAATTGTAGGGTATTCTCTGGTTTTAATGGCAGATTGCCCTTCAATCTTGACTTGAATAAGGCAATGGAGGCTTTGTATGGGACTAGCTCTACTAGAAGGTGGAGAGGAAAGGTTCTAATTCTGGGGCTTGTGGGTTTTGTTACAATCATTTGGGGTTTCATGAGTTTCAATGATGGAAGTTTGGGGATGAGAGAGAAAGCCCCAGATATGGGTGGAGAGAAAGCTTGGATCCTGCAGCAGCATTTCAATATCAGCAAGTCCCAGGTTCTCGCTTTCGCTTCGTTGTTCTCCGAATCAGATCAGATAACATCCCTTGAATGTACCAAAGAACCAGGACCACGAATGATGTTAAGTAATGTCATTTCCTGTTCTCTGAAGGTGCTATGTTCAGATGAGGAGGCAGAAAATGAAGAAGCCAAGAACCAATGCCTGGTTCAAGATGAGAGCATGCCCAAGAAGCGTGATGTATCATTGCTTGAGGACAAATCCATATCATGCACCACGCAATCTACAGTTTCAGCAAATAGGATTTTTGGAAAGAACAATTTGGGTTCTGTGGCACAGGAGTGTGGAAAAGAGGACTCGCAGTTGCTTTGTACATTGGTAATCCGATGGTGGTGGGTTTTTGTTGGAATGATCATGTGCTACAAACTGTCTGGTTTTTCTATGAAATTGTGGAGGAATAAAAAACAGAAGGTGGGTCAAAAGGTTCAAGAGCAGCCATTGCCTCGGAGGAAGCAGCCAGAGAAACTGCAACAGCACGCCCAAAAGCCTCCTAAGGTTGCTGGCACGTGGAGGACGAATCTACTTATAGTATTTATCATATTTGGCTGCATTACATCATTGTGGTTACTGTGTCACTTATGTGCAAGAGATCATTTGTGGAGGAAAGAAACACTTTCCAACATGTGCGACGAACGAGCCCGGATGTTGCAGGATCAGTTTAATGTGAGCCTGAATCATGTTCATGCTTTAGCTATTCTTGTATCCACCTTTCACCATGGGAAACAACCTACGGCAATTGATCAGAAAACATTTGGTGAATATACAGAGAGAACATCTTTTGAGAGACCCCTTACGAGTGGTGTTGCTTATGCTTTGAAAGTTACTCATGCTGAGAGGGAACATTTTGAGAAGGAACATGGATGGACAATAAAAAAAATGGAAACTGAGGACCAGACTCTAGTCCAAGATTTTATTACGGAAAGCTTGGCCTCTGCGCCTATTCAAGATGAATATGCGCCTGTGATATTTTCTCAAGAAACTGTCTCCCACATTGTCTCTATTGACATGATGTCCGGAAAGGACGACCGTGAGAACATCTTGCGAGCAAGGGCAACTGGAAAGGGAGTACTGACCTCCCCGTTTAAGCTATTGAAATCCAATCACCTGGGAGTTGTACTCACATTCGCAGTCTATAATGCTGATTTGCCTCCAGATGCCACATCAGAGCAACGTATTCAAGCTACTGTGGGGTATCTAGGTGCATCTTATGATGTTCCATCGCTGGTGGAGAAGCTCTTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATGCATCTGCTCCGATCAACATGTATGGTACTGATGTTATTGATACTGGCCTGTTGCACATTAGCAACCTTGATTTTGGGGATCCACAACGCAAACATGAAATGCACTGCAGGTTTAAGCACAAACCTCCATTTCCTTTGACAGCAGCAAGTGCATCAATAGGAGTCCTAGTCATTACCTTGCTAGTTAGTCATATTTTTTATGCGGCCATAAGCCGAATAGCGAAAGTGGAGGCAGACTATCGTAAGATGATGGAACTCAAAGTTCGTGCAGAAGCTGCTGATGTGGCCAAATCTCAGTTCCTTGCAACTGTTTCCCATGAGATCAGGACACCAATGAATGGTGTTTTAGGCATGCTGCAAATGCTGATGGACACCGATCTAGGTCCAAACCAACAGGACTATGCTGAGACTGCTCATGCTAGCGGGAGAGAATTGATATCGCTGATAAATGAGGTTCTTGATCAGGCAAAAATTGAATCAGGCAGGCTGGAACTTGAAACCGTACCTTTTGATTTGCGTTCAGTGCTTGATAATGTTCTATCACTTAACTCAGGAAAATCTAATGAGAAAGGAATTGAGTTGGCTGCTTATGTTTCCAATATGGTACCTGAAGTTGTCATTGGTGATCCTGGACGCTTCCGGCAAATAATTACAAATCTTGTTGGAAATTCAATTAAGTTCACCCATGACAAAGGACACATATTTGTCTCAGTGCATCTGGTGGATGAAGTGAGGGCCCCACCTGATCTTATGGATGACGTTCTAAGACAAGGCTTGAACTTAGTTGGAGACGTCTCAAATAAAACTTATAATACCTTGAGTGGCTTTCCTGTTGTCAACAGATGGAAAAGCTGGGAAGATTTTAAGTCAATATGCAGCACAACTCCGGAGGAATCTGATATGATCAAATTACTAGTAACAGTTGAGGATACAGGTGTAGGAATCCCGTTAGAAGCTCAAAGTCGCATTTTCACACCTTTTATGCAGGCTGATAGCTCCACGTCTAGAACTTATGGCGGTACTGGAATAGGATTGAGCATTAGTAAACGTCTGGTAGATCTCATGAGCGGGGAGATAGGGTTTGTAAGCGAACCTGGCACTGGCAGTACCTTTTCATTTACGGGATCTTTTGGGAAGGGAGAAGTAAGTTCCCTAGAGACAAAGTGGCACCAGTATGAACCAGCTGTCTCAGAGTTTCAAGGACTGAGAGCATTGGTAATAGATAATAAAACCATCCGAACTGAGGTCACGAAATACCATATTCAGAGATTGGGAATATCTGTGGATACAGCATCCTGTCTGGAATCAGCGTGCGTTTATCTATCTAGTGCCGGCAACACCAGTCTTTCCACAGAGTTGGCCATGGTTGTTATTGACAAAGATGTTTGGGATAAAGAAGCTGGTATTATATTACATCATTCAATTAAAGAGCACAGGAAAAACAATGTGGAATCCCTCATAAACCTTCCGAAGATACTTCTCTTGGCTAACTCCATCACCCCTGTTGAACGCAATGTAGTAACATCTTTGGGTCTTGTAGATAAAGTATTGACAAAGCCACTTCGGTTAAGTGTCTTAATCGCTTGCTTTCAAGAAGCCCTTGGAAGTCGTAAGAAGAGGCTTCTAAATGCAAACAAACCAACTCTTGGAAAGTTACTGAGAGAGAGGAGAATTTTGGTGGTGGATGACAATGTAGTAAACAGAAGAGTGGCAGAAGGTGCTTTAAAGAAGTATGGAGCAATTGTCACCTGTGTGGAGAGTGGAAGGGCTGCTTTGTTGAAGCTTAAGCCACCCCACAACTTTGATGCTTGCTTTATGGATCTCCAGATGCCAGAAATGGACGGGTTTGAAGCGACTCGGCTCATCCGTGGTATGGAGAGTGAGGTAAATGAGAAAATTGCAGCTGGGGAAGCATCAGTTGAGATATTTGGCAATGTCGAAAATTGGCACACGCCAATATTGGCTATGACGGCTGATGTGATTCAGGCTTCAAATGAAGAGTGCATGAAATGTGGGATGGATGATTATGTGTCAAAGCCATTTGAAGAAGAACAACTGTATTCAGCAGTGGCACGTTTCTTTGAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1227

Amino Acids

136.52

Weight (kDa)

6.27

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 343 - 537 6.2e-36 CHASE domain
HisKA PF00512 625 - 690 7.8e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 832 - 903 5.2e-18 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 926 - 1064 2.7e-34 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 1087 - 1219 9.7e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2397
AatII GACGTC 1 cut(s) 2382
Acc36I ACCTGC 1 cut(s) 1686
Acc65I GGTACC 1 cut(s) 2191
AccB1I GGYRCC 2 cut(s) 2191, 2754
AccB7I CCANNNNNTGG 3 cut(s) 439, 1925, 3233
AccBSI CCGCTC 1 cut(s) 2655
AccIII TCCGGA 2 cut(s) 1307, 2473
AciI CCGC 4 cut(s) 1790, 2010, 2604, 2655
AclWI GGATC 8 cut(s) 232, 245, 954, 1661, 1674, 2207, 2719, 3414
AcoI YGGCCR 3 cut(s) 1791, 1872, 2946
AcsI RAATTY 2 cut(s) 2254, 3258
AcuI CTGAAG 2 cut(s) 404, 2217
AcvI CACGTG 1 cut(s) 801
AcyI GRCGYC 1 cut(s) 2379
AfaI GTAC 9 cut(s) 331, 607, 1362, 1410, 1615, 2091, 2193, 2608, 2695
AfiI CCNNNNNNNGG 8 cut(s) 217, 218, 439, 1925, 2687, 2830, 3233, 3460
AflII CTTAAG 1 cut(s) 3371
AflIII ACRYGT 2 cut(s) 915, 1605
AhdI GACNNNNNGTC 1 cut(s) 1303
AhlI ACTAGT 1 cut(s) 2500
AjiI CACGTC 1 cut(s) 2589
AjnI CCWGG 6 cut(s) 270, 340, 438, 1398, 2215, 2680
AjuI GAANNNNNNNTTGG 6 cut(s) 426, 458, 1868, 1900, 2190, 2222
AleI CACNNNNGTG 3 cut(s) 574, 873, 3338
Alw21I GWGCWC 1 cut(s) 3021
Alw26I GTCTC 9 cut(s) 1072, 1282, 1294, 1988, 2299, 2370, 2386, 2738, 2779
AlwI GGATC 8 cut(s) 232, 245, 954, 1661, 1674, 2207, 2719, 3414
AlwNI CAGNNNCTG 1 cut(s) 1862
Aor13HI TCCGGA 2 cut(s) 1307, 2473
AoxI GGCC 6 cut(s) 1221, 1633, 1791, 1872, 2322, 2946
ApaI GGGCCC 1 cut(s) 2326
ApoI RAATTY 2 cut(s) 2254, 3258
Asp718I GGTACC 1 cut(s) 2191
AspLEI GCGC 2 cut(s) 1231, 1252
AspS9I GGNCC 5 cut(s) 344, 1183, 1970, 2322, 2323
AsuC2I CCSGG 1 cut(s) 934
AsuHPI GGTGA 7 cut(s) 998, 1061, 1388, 2222, 2260, 3076, 3325
AsuNHI GCTAGC 1 cut(s) 2006
AvaII GGWCC 3 cut(s) 344, 1183, 1970
AxyI CCTNAGG 1 cut(s) 786
BaeGI GKGCMC 1 cut(s) 2326
BaeI ACNNNNGTAYC 4 cut(s) 1605, 1638, 2508, 2541
BalI TGGCCA 2 cut(s) 1874, 2948
BamHI GGATCC 2 cut(s) 237, 1666
BanI GGYRCC 2 cut(s) 2191, 2754
BanII GRGCYC 2 cut(s) 934, 2326
BbrPI CACGTG 1 cut(s) 801
Bbv12I GWGCWC 1 cut(s) 3021
BceAI ACGGC 2 cut(s) 1041, 3573
BciT130I CCWGG 6 cut(s) 272, 342, 440, 1400, 2217, 2682
BciVI GTATCC 3 cut(s) 1006, 2509, 2866
BclI TGATCA 4 cut(s) 645, 1033, 2047, 2490
BcnI CCSGG 1 cut(s) 934
BcoDI GTCTC 9 cut(s) 1072, 1282, 1294, 1988, 2299, 2370, 2386, 2738, 2779
BcuI ACTAGT 1 cut(s) 2500
BfmI CTRYAG 3 cut(s) 242, 519, 1693
BfrI CTTAAG 1 cut(s) 3371
BfuAI ACCTGC 1 cut(s) 1686
BfuI GTATCC 3 cut(s) 1006, 2509, 2866
BglII AGATCT 1 cut(s) 2644
BmcAI AGTACT 1 cut(s) 1362
Bme1390I CCNGG 7 cut(s) 272, 342, 440, 934, 1400, 2217, 2682
Bme18I GGWCC 3 cut(s) 344, 1183, 1970
BmeRI GACNNNNNGTC 1 cut(s) 1303
BmgBI CACGTC 1 cut(s) 2589
BmgT120I GGNCC 5 cut(s) 344, 1183, 1970, 2322, 2323
BmiI GGNNCC 6 cut(s) 239, 1668, 2193, 2324, 2325, 2756
BmrFI CCNGG 7 cut(s) 272, 342, 440, 934, 1400, 2217, 2682
BmsI GCATC 9 cut(s) 1438, 1505, 1598, 1748, 2311, 2888, 3382, 3405, 3509
BmtI GCTAGC 1 cut(s) 2010
BoxI GACNNNNGTC 1 cut(s) 1193
BplI GAGNNNNNCTC 2 cut(s) 1395, 1427
BpmI CTGGAG 2 cut(s) 1428, 3395
BpuEI CTTGAG 3 cut(s) 506, 1251, 2425
BpuMI CCSGG 1 cut(s) 934
BsaAI YACGTR 1 cut(s) 801
BsaHI GRCGYC 1 cut(s) 2379
BsaI GGTCTC 1 cut(s) 1072
BsaJI CCNNGG 7 cut(s) 270, 737, 1009, 1399, 2949, 3187, 3454
BsaWI WCCGGW 2 cut(s) 1307, 2473
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 1740, 1770, 2651, 2681, 3315, 3345
Bsc4I CCNNNNNNNGG 8 cut(s) 217, 218, 439, 1925, 2687, 2830, 3233, 3460
Bse118I RCCGGY 1 cut(s) 2918
Bse1I ACTGG 6 cut(s) 1354, 1636, 2614, 2692, 2758, 2928
Bse21I CCTNAGG 1 cut(s) 786
Bse3DI GCAATG 5 cut(s) 78, 479, 731, 3106, 3526
BseAI TCCGGA 2 cut(s) 1307, 2473
BseBI CCWGG 6 cut(s) 272, 342, 440, 1400, 2217, 2682
BseDI CCNNGG 7 cut(s) 270, 737, 1009, 1399, 2949, 3187, 3454
BseLI CCNNNNNNNGG 8 cut(s) 217, 218, 439, 1925, 2687, 2830, 3233, 3460
BseMI GCAATG 5 cut(s) 78, 479, 731, 3106, 3526
BseNI ACTGG 6 cut(s) 1354, 1636, 2614, 2692, 2758, 2928
BseRI GAGGAG 2 cut(s) 419, 3268
BseSI GKGCMC 1 cut(s) 2326
BseYI CCCAGC 2 cut(s) 2439, 3491
BsgI GTGCAG 1 cut(s) 1875
Bsh1285I CGRYCG 1 cut(s) 1321
BshFI GGCC 6 cut(s) 1223, 1635, 1793, 1874, 2324, 2948
BshNI GGYRCC 2 cut(s) 2191, 2754
BsiEI CGRYCG 1 cut(s) 1321
BsiHKAI GWGCWC 1 cut(s) 3021
BsiSI CCGG 5 cut(s) 934, 1308, 2227, 2474, 2919
BslFI GGGAC 2 cut(s) 103, 253
BslI CCNNNNNNNGG 8 cut(s) 217, 218, 439, 1925, 2687, 2830, 3233, 3460
BsmAI GTCTC 9 cut(s) 1072, 1282, 1294, 1988, 2299, 2370, 2386, 2738, 2779
BsmBI CGTCTC 2 cut(s) 2370, 2386
BsmFI GGGAC 2 cut(s) 103, 253
BsnI GGCC 6 cut(s) 1223, 1635, 1793, 1874, 2324, 2948
Bso31I GGTCTC 1 cut(s) 1072
Bsp120I GGGCCC 1 cut(s) 2322
Bsp1286I GDGCHC 3 cut(s) 934, 2326, 3021
Bsp13I TCCGGA 2 cut(s) 1307, 2473
Bsp1407I TGTACA 1 cut(s) 605
Bsp19I CCATGG 2 cut(s) 1009, 2949
BspACI CCGC 4 cut(s) 1790, 2010, 2604, 2655
BspANI GGCC 6 cut(s) 1223, 1635, 1793, 1874, 2324, 2948
BspEI TCCGGA 2 cut(s) 1307, 2473
BspHI TCATGA 2 cut(s) 168, 2649
BspLI GGNNCC 6 cut(s) 239, 1668, 2193, 2324, 2325, 2756
BspMAI CTGCAG 2 cut(s) 246, 1697
BspMI ACCTGC 1 cut(s) 1686
BspOI GCTAGC 1 cut(s) 2010
BspPI GGATC 8 cut(s) 232, 245, 954, 1661, 1674, 2207, 2719, 3414
BspT107I GGYRCC 2 cut(s) 2191, 2754
BspTI CTTAAG 1 cut(s) 3371
BspTNI GGTCTC 1 cut(s) 1072
BsrBI CCGCTC 1 cut(s) 2655
BsrDI GCAATG 5 cut(s) 78, 479, 731, 3106, 3526
BsrFI RCCGGY 1 cut(s) 2918
BsrGI TGTACA 1 cut(s) 605
BsrI ACTGG 6 cut(s) 1354, 1636, 2614, 2692, 2758, 2928
BssAI RCCGGY 1 cut(s) 2918
BssECI CCNNGG 7 cut(s) 270, 737, 1009, 1399, 2949, 3187, 3454
BssNI GRCGYC 1 cut(s) 2379
BssT1I CCWWGG 3 cut(s) 1009, 2949, 3187
Bst2UI CCWGG 6 cut(s) 272, 342, 440, 1400, 2217, 2682
Bst6I CTCTTC 3 cut(s) 3197, 3286, 3579
BstACI GRCGYC 1 cut(s) 2379
BstAFI CTTAAG 1 cut(s) 3371
BstAPI GCANNNNNTGC 4 cut(s) 733, 1736, 1862, 3305
BstAUI TGTACA 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 801
BstDSI CCRYGG 3 cut(s) 1009, 2949, 3454
BstHHI GCGC 2 cut(s) 1231, 1252
BstMAI GTCTC 9 cut(s) 1072, 1282, 1294, 1988, 2299, 2370, 2386, 2738, 2779
BstMCI CGRYCG 1 cut(s) 1321
BstNI CCWGG 6 cut(s) 272, 342, 440, 1400, 2217, 2682
BstNSI RCATGY 4 cut(s) 460, 919, 1609, 1942
BstPAI GACNNNNGTC 1 cut(s) 1193
BstSCI CCNGG 7 cut(s) 270, 340, 438, 932, 1398, 2215, 2680
BstSFI CTRYAG 3 cut(s) 242, 519, 1693
BstSLI GKGCMC 1 cut(s) 2326
BstX2I RGATCY 5 cut(s) 237, 1666, 2644, 2711, 3406
BstXI CCANNNNNNTGG 2 cut(s) 1399, 2944
BstYI RGATCY 5 cut(s) 237, 1666, 2644, 2711, 3406
Bsu36I CCTNAGG 1 cut(s) 786
BsuI GTATCC 3 cut(s) 1006, 2509, 2866
BsuRI GGCC 6 cut(s) 1223, 1635, 1793, 1874, 2324, 2948
BtgI CCRYGG 3 cut(s) 1009, 2949, 3454
BtgZI GCGATG 1 cut(s) 1497
BtrI CACGTC 1 cut(s) 2589
BtsI GCAGTG 2 cut(s) 1690, 3660
BtsIMutI CAGTG 5 cut(s) 1690, 2115, 2304, 2685, 3660
BveI ACCTGC 1 cut(s) 1686
CaiI CAGNNNCTG 1 cut(s) 1862
CciI TCATGA 2 cut(s) 168, 2649
CfoI GCGC 2 cut(s) 1231, 1252
Cfr10I RCCGGY 1 cut(s) 2918
Cfr13I GGNCC 5 cut(s) 344, 1183, 1970, 2322, 2323
CseI GACGC 1 cut(s) 2229
Csp6I GTAC 9 cut(s) 330, 606, 1361, 1409, 1614, 2090, 2192, 2607, 2694
CviQI GTAC 9 cut(s) 330, 606, 1361, 1409, 1614, 2090, 2192, 2607, 2694
DraI TTTAAA 1 cut(s) 3312
DriI GACNNNNNGTC 1 cut(s) 1303
EaeI YGGCCR 3 cut(s) 1791, 1872, 2946
Eam1104I CTCTTC 3 cut(s) 3197, 3286, 3579
Eam1105I GACNNNNNGTC 1 cut(s) 1303
EarI CTCTTC 3 cut(s) 3197, 3286, 3579
Eco130I CCWWGG 3 cut(s) 1009, 2949, 3187
Eco24I GRGCYC 2 cut(s) 934, 2326
Eco31I GGTCTC 1 cut(s) 1072
Eco32I GATATC 1 cut(s) 2025
Eco47I GGWCC 3 cut(s) 344, 1183, 1970
Eco57I CTGAAG 2 cut(s) 404, 2217
Eco72I CACGTG 1 cut(s) 801
Eco81I CCTNAGG 1 cut(s) 786
EcoO109I RGGNCCY 2 cut(s) 2322, 2323
EcoRII CCWGG 6 cut(s) 270, 340, 438, 1398, 2215, 2680
EcoRV GATATC 1 cut(s) 2025
EcoT14I CCWWGG 3 cut(s) 1009, 2949, 3187
EcoT22I ATGCAT 1 cut(s) 1591
EcoT38I GRGCYC 2 cut(s) 934, 2326
ErhI CCWWGG 3 cut(s) 1009, 2949, 3187
Esp3I CGTCTC 2 cut(s) 2370, 2386
FalI AAGNNNNNCTT 6 cut(s) 891, 923, 3171, 3203, 3343, 3375
FaqI GGGAC 2 cut(s) 103, 253
FauI CCCGC 2 cut(s) 2003, 2648
FbaI TGATCA 4 cut(s) 645, 1033, 2047, 2490
FriOI GRGCYC 2 cut(s) 934, 2326
GlaI GCGC 2 cut(s) 1230, 1251
GsaI CCCAGC 2 cut(s) 2443, 3495
GsuI CTGGAG 2 cut(s) 1428, 3395
HaeIII GGCC 6 cut(s) 1223, 1635, 1793, 1874, 2324, 2948
HapII CCGG 5 cut(s) 934, 1308, 2227, 2474, 2919
HgaI GACGC 1 cut(s) 2229
HhaI GCGC 2 cut(s) 1231, 1252
Hin1I GRCGYC 1 cut(s) 2379
Hin6I GCGC 2 cut(s) 1229, 1250
HinP1I GCGC 2 cut(s) 1229, 1250
HincII GTYRAC 1 cut(s) 2425
HindII GTYRAC 1 cut(s) 2425
HindIII AAGCTT 3 cut(s) 231, 1216, 3368
HpaII CCGG 5 cut(s) 934, 1308, 2227, 2474, 2919
HphI GGTGA 7 cut(s) 998, 1061, 1388, 2222, 2260, 3076, 3325
Hpy166II GTNNAC 4 cut(s) 2268, 2425, 2633, 3286
Hpy8I GTNNAC 4 cut(s) 2268, 2425, 2633, 3286
Hpy99I CGWCG 1 cut(s) 926
HpyAV CCTTC 9 cut(s) 58, 99, 379, 697, 1138, 2716, 3062, 3296, 3345
HpyCH4IV ACGT 7 cut(s) 800, 1465, 2346, 2379, 2588, 2635, 3661
HpySE526I ACGT 7 cut(s) 800, 1465, 2346, 2379, 2588, 2635, 3661
Hsp92I GRCGYC 1 cut(s) 2379
HspAI GCGC 2 cut(s) 1229, 1250
Kpn2I TCCGGA 2 cut(s) 1307, 2473
KpnI GGTACC 1 cut(s) 2195
KroI GCCGGC 1 cut(s) 2918
KroNI GCCGGC 1 cut(s) 2920
Ksp22I TGATCA 4 cut(s) 645, 1033, 2047, 2490
LmnI GCTCC 3 cut(s) 1600, 2588, 3323
LweI GCATC 9 cut(s) 1438, 1505, 1598, 1748, 2311, 2888, 3382, 3405, 3509
MaeII ACGT 7 cut(s) 800, 1465, 2346, 2379, 2588, 2635, 3661
MaeIII GTNAC 9 cut(s) 148, 858, 866, 1114, 2503, 2833, 3106, 3240, 3331
MbiI CCGCTC 1 cut(s) 2655
MboII GAAGA 8 cut(s) 431, 2456, 3070, 3214, 3303, 3596, 3644, 3647
MfeI CAATTG 3 cut(s) 1029, 1557, 3327
MflI RGATCY 5 cut(s) 237, 1666, 2644, 2711, 3406
MhlI GDGCHC 3 cut(s) 934, 2326, 3021
MlsI TGGCCA 2 cut(s) 1874, 2948
MluNI TGGCCA 2 cut(s) 1874, 2948
MlyI GAGTC 5 cut(s) 584, 1183, 1758, 3436, 3680
MmeI TCCRAC 4 cut(s) 619, 936, 2230, 2353
Mox20I TGGCCA 2 cut(s) 1874, 2948
Mph1103I ATGCAT 1 cut(s) 1591
MroI TCCGGA 2 cut(s) 1307, 2473
MroNI GCCGGC 1 cut(s) 2918
MscI TGGCCA 2 cut(s) 1874, 2948
MslI CAYNNNNRTG 6 cut(s) 574, 853, 873, 975, 2760, 3338
Msp20I TGGCCA 2 cut(s) 1874, 2948
MspA1I CMGCKG 2 cut(s) 2771, 3491
MspCI CTTAAG 1 cut(s) 3371
MspI CCGG 5 cut(s) 934, 1308, 2227, 2474, 2919
MspR9I CCNGG 7 cut(s) 272, 342, 440, 934, 1400, 2217, 2682
MunI CAATTG 3 cut(s) 1029, 1557, 3327
MvaI CCWGG 6 cut(s) 272, 342, 440, 1400, 2217, 2682
NaeI GCCGGC 1 cut(s) 2920
NciI CCSGG 1 cut(s) 934
NcoI CCATGG 2 cut(s) 1009, 2949
NgoMIV GCCGGC 1 cut(s) 2918
NheI GCTAGC 1 cut(s) 2006
NlaIV GGNNCC 6 cut(s) 239, 1668, 2193, 2324, 2325, 2756
NmeAIII GCCGAG 1 cut(s) 3424
NmuCI GTSAC 3 cut(s) 866, 2833, 3331
NsiI ATGCAT 1 cut(s) 1591
NspI RCATGY 4 cut(s) 460, 919, 1609, 1942
OliI CACNNNNGTG 3 cut(s) 574, 873, 3338
PaeI GCATGC 2 cut(s) 460, 1942
PagI TCATGA 2 cut(s) 168, 2649
PciI ACATGT 2 cut(s) 915, 1605
PcsI WCGNNNNNNNCGW 2 cut(s) 806, 3451
PdiI GCCGGC 1 cut(s) 2920
PfeI GAWTC 9 cut(s) 302, 811, 967, 2063, 2480, 2529, 2891, 3038, 3568
PflMI CCANNNNNTGG 3 cut(s) 439, 1925, 3233
PfoI TCCNGGA 1 cut(s) 2215
PleI GAGTC 5 cut(s) 584, 1183, 1757, 3436, 3679
PmaCI CACGTG 1 cut(s) 801
PmlI CACGTG 1 cut(s) 801
PpsI GAGTC 5 cut(s) 584, 1183, 1757, 3436, 3679
Ppu21I YACGTR 1 cut(s) 801
PscI ACATGT 2 cut(s) 915, 1605
PshAI GACNNNNGTC 1 cut(s) 1193
PsiI TTATAA 1 cut(s) 2397
Psp6I CCWGG 6 cut(s) 270, 340, 438, 1398, 2215, 2680
PspCI CACGTG 1 cut(s) 801
PspFI CCCAGC 2 cut(s) 2439, 3491
PspGI CCWGG 6 cut(s) 270, 340, 438, 1398, 2215, 2680
PspN4I GGNNCC 6 cut(s) 239, 1668, 2193, 2324, 2325, 2756
PspOMI GGGCCC 1 cut(s) 2322
PspPI GGNCC 5 cut(s) 344, 1183, 1970, 2322, 2323
PstI CTGCAG 2 cut(s) 246, 1697
PstNI CAGNNNCTG 1 cut(s) 1862
PsuI RGATCY 5 cut(s) 237, 1666, 2644, 2711, 3406
PvuII CAGCTG 2 cut(s) 2771, 3491
RsaI GTAC 9 cut(s) 331, 607, 1362, 1410, 1615, 2091, 2193, 2608, 2695
RsaNI GTAC 9 cut(s) 330, 606, 1361, 1409, 1614, 2090, 2192, 2607, 2694
RseI CAYNNNNRTG 6 cut(s) 574, 853, 873, 975, 2760, 3338
Sau96I GGNCC 5 cut(s) 344, 1183, 1970, 2322, 2323
ScaI AGTACT 1 cut(s) 1362
SchI GAGTC 5 cut(s) 584, 1183, 1758, 3436, 3680
ScrFI CCNGG 7 cut(s) 272, 342, 440, 934, 1400, 2217, 2682
SduI GDGCHC 3 cut(s) 934, 2326, 3021
SfaNI GCATC 9 cut(s) 1438, 1505, 1598, 1748, 2311, 2888, 3382, 3405, 3509
SfcI CTRYAG 3 cut(s) 242, 519, 1693
SinI GGWCC 3 cut(s) 344, 1183, 1970
SmiMI CAYNNNNRTG 6 cut(s) 574, 853, 873, 975, 2760, 3338
SmlI CTYRAG 4 cut(s) 485, 1266, 2404, 3371
SmoI CTYRAG 4 cut(s) 485, 1266, 2404, 3371
SpeI ACTAGT 1 cut(s) 2500
SphI GCATGC 2 cut(s) 460, 1942
SsiI CCGC 4 cut(s) 1790, 2010, 2604, 2655
SspI AATATT 1 cut(s) 3546
StyD4I CCNGG 7 cut(s) 270, 340, 438, 932, 1398, 2215, 2680
StyI CCWWGG 3 cut(s) 1009, 2949, 3187
TaiI ACGT 7 cut(s) 803, 1468, 2349, 2382, 2591, 2638, 3664
TaqI TCGA 1 cut(s) 3525
TatI WGTACW 3 cut(s) 605, 1360, 1408
TauI GCSGC 1 cut(s) 1793
TfiI GAWTC 9 cut(s) 302, 811, 967, 2063, 2480, 2529, 2891, 3038, 3568
TscAI CASTG 5 cut(s) 1697, 2115, 2304, 2692, 3660
TseFI GTSAC 3 cut(s) 866, 2833, 3331
Tsp45I GTSAC 3 cut(s) 866, 2833, 3331
TspGWI ACGGA 2 cut(s) 1226, 3443
TspRI CASTG 5 cut(s) 1697, 2115, 2304, 2692, 3660
Van91I CCANNNNNTGG 3 cut(s) 439, 1925, 3233
Vha464I CTTAAG 1 cut(s) 3371
VpaK11BI GGWCC 3 cut(s) 344, 1183, 1970
XapI RAATTY 2 cut(s) 2254, 3258
XbaI TCTAGA 1 cut(s) 2591
XceI RCATGY 4 cut(s) 460, 919, 1609, 1942
ZraI GACGTC 1 cut(s) 2380
ZrmI AGTACT 1 cut(s) 1362
Zsp2I ATGCAT 1 cut(s) 1591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.