Rroxscaffold_2G00092770

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003045
Physical Location & Seq
Reverse (-)
14255288 .. 14267303
12016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092770.1

Sequence Viewer

Length: 3732 bp
ATGTATCTGAATTGCAGGGTGTCTGGTTTTAATGGCAGACTACCTGCTAATTTCAAGTTAAACAAGGCAATGGAGCCCCTGGATGGGCCTAATTGTGTCAGTTATTCGAGAAGAAAGCTTCTACTTCTTGGGATTTTAGGTTTTATTACAGTAGTGTGGTTTTTCCTGGGTTTCAATGAGGGACTTCTGGGGATGAAAGAGAAGACTCCAGAGATGTGTGGAGAGAAAGCTCGAATCTTGCAGCAGCATTTCAATGTCAGCAAGAACCAGCTTCTGGCTTTAGCTTCCTTGTTCTCCGAATCAGATCAGATAGCATCCCTTGAATGTAACAAAGAAACAGGACATGGGATGCTTTTAACTGATGGCATTTCCTGTGCTTTGAAGGCAGTATGTTCAGATGAGAAAGAATTTCAAAAGCATCATAAATGGGTGGCAGAATATGTTGAAGCCAAGGACCAATGCCTGGTTCAAGATGAAAACATCCCCAGGAGGCTTGACCTATCAGTGCTACAGGAAAAGTCTTTGGCACACATTCCACAGTCTACAGTATCTGCTAATAGAATTTGTCAGAAGGACAATTTGGGATCAGGGGCAAAGGTAGAGTGCACAAAAGTGGATTCTCAGATTTTATGTTGTTTGATAAAGGGTTGTTCGTGGGTTTTTGTTGGAATGATTTTGTTCTACCAATTGTCTGGTTTTTCTTTGAAATTATGGAGGAACCAGAAGCAGAAGCTGGTTCATGAGTGCCCATGCCAACAGAAATTGGTTCAAGAGCAGCCATTGGGTCGTAAGAAGCAGCCAGAGAAACAGCTACAGCAAGTCCACAGTCCTCCTAAGGTTGCTTGCACGTGGAGGAAGAAACTCCTAATCGTATTTGTCTTATTTGGAGTTATTACGTCAATTTGGTTATTTTTCCACTTGAATGAAAGAGATTTTGTGTGGAGAGAAGAAACACTTTCCAACATGTGTGATGAACGAGCCCGGATGTTGCAAGATCAGTTCAATGTGAGCCTGAATCATGTTCATGCTTTAGCTATTCTCGTCTCCACCTTTCACCATGGAAAGCATCCTTCTGCCATTGATCAGAAAACATTTGGTGAATATACAGAGAGAACAGCTTTTGAGAGACCACTTACTAGTGGTGTTGCTTATGCTTTAAAAGTTACTCATGCTGAGAGGGAACAATTCGAGAAGGAACATGGATGGACAATAAAGAAAATGGAAACTGAGGACCAGACTCTTGTCCAAGATTTTCTTCCAGAAAGCTTGGATCCTGCCCCTATTCAAGATGAATATGCGCCTGTGATATTTTCTCAAGAAACTGTCTCCCATATTGTCTCCATTGACATGATGTCGGGAAAGGATGACCGCGAGAACATCTTGCGAGCAAGGTCAACAGGAAAGGGAGTACTGACTTCCCCTTTTAAGCTATTGAAATCCAATCACCTGGGAGTTGTTCTTACATTTGCTGTGTATAATATGGACTTGCCTCCAGATGCCACATCAGAGCAACGAGTTCAAGCGACTGTGGGGTATCTAGGTGCATCTTACGATGTCCCATCACTGGTGGAGAAGCTCCTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACTACTAATGCATCTTCTCTGATCAACATGTATGGAACTGATGTTATTGATACTGGGCTGTTGCACATTAGCAGCCTTGATTTTGGTGATCCACAACGCAAACATGAAATGCACTGCAGGTTTAAGCATAAACCTCCTTTTCCTTGGACAGCAGTAAGTGCAGCAGTGGGAGTCCTAGTCATTACCTTGCTAGTTGGTCATATTTTTCATGCGGCCATAAGCCGAATAGCAAAGGTAGAGGCAGACTATTGTGTGATGGAACAACTCAAAGTTCGTGCAGAGGCTGCAGATGTGGCCAAATCACAGTTCCTTGCGACTGTTTCCCATGAAATCCGGACACCAATGAATGGTGTTTTAGGTATGCTGCAAATGCTGATGGACACAAATCTTGATGCCAACCAACAGGATTATGCTGAGACTGCGCATGCTAGTGGAAGAGATCTCATATCGCTGATAAATGAGGTTCTTGATCAGGCCAAAATAGAATCAGGCCGGCTGGAACTTGAAACTGTACCTTTTGATCTTCGTTCAGTGCTTGATAATGTTCTATCACTTTTCTCAGGAAAAACTAATGAGAAAGGGATTGAGTTGGCTGTCTACGTCTCCAATATGGTCCCTGAAGTTGTCATCGGAGACCCAGGACGCCTTCGGCAGATTATTACAAACCTTGTTGGGAATTCAATCAAGTTCACCCATGACAAAGGACATATATTTGTCTCGGTGCATCTGGCGGATGAAGTGAAGGGTCCACCTGATTTTATGGACGAAGTTCTAAGACAAGGCTTGAATTTAGTTGGAGACCTCTCAAACAAAACTTATAATACATTGAGTGGCTTTCCTGTGGTTGATAGATGGAAAAGTTGGGAACGTTTTAAAACATTAAGCAGCACAACAATGGAGGAACCTGATATGATAAAATTAATTGTAACAGTTGAGGATACAGGTGTAGGAATTCCACTAGATGCACAAAGTCGCATCTTCACGCCTTTTATGCAGGCCGATAGTTCCACTTCTAGAACTTATGGTGGCACTGGAATAGGATTGAGCATTAGCAAACGTTTGGTCGATCTCATGTGTGGGGAGATAGGGTTTGTGAGTGAACCTGGCATTGGCAGTACCTTTTCATTTACTGGGTCGTTTCTGAGAGGGAAGACAAGTTCTCTAGATATGAAGTGGCCTCAGTATGAACCAGCTGTCTCAGAATTTCGAGGACTGAGAGCACTGGTAATAGATAAGAGAATCACCCGAACTGAAGTCACAAGATATCATATGCAGAGGTTGGGAATATCGGCTGATATTGCTTCCAGTCTTGAATCAGCATGCTCTTATCTATCTAGTACGAGCAAGACAAGCCTATCCACATGTTTGGCCATGGTTCTTATCGACAAAGATGTTTGGGATAAAGAAACTGGTGTTACGTTCCATCAGTCAGTTAAAGAGCACAGGCAAAACAGCAGCGTGGAGTCTCCCGTGAACTTTCCAAAGATATTTCTCCTGGCTACCTCCATTAGCTCTACTGAACGTAATGAACTAAAATCTTCTGGTCTTGTAGATAATGTATTGATGAAGCCTCTTCGGTTAAGTGTCTTAATTGCCTGCTTCCAAGAGGCCCTTGTAAGCGGTAAAACGAGGCTTGTGAACATAAAAAAACCAACTCTTGGAAAGTTACTACAAGGCAGGAAGATTTTGGTAGTGGATGATAATGCAGTCAACAGAAGAGTCGCAGAAGGTGCTTTAAAGAAATATGGAGCAATTGTCACCTGTGTTGATAGCGGAAAGACTGCTTTAGACATGCTTAAGCCACCACACAACTTTGACGCTTGCTTTATGGACCTCCAAATGCCAGAAATGGATGGATTCGAAGCAACTCGTCGAATTCGCTGTCTGGAGAGTGAGGTTAATGAGAAAATTGCATCTGGGGAAGCACCAATCGAGATGTTTGGCAATGTGGAGAATTGGCACACACCAATTTTAGCTATGACAGCTGATGTGATTCAGGCTTCAAATGAAGAATGTATGAATTGTGGGATGGATGGCTATGTGTCAAAGCCATTTGAAGAAGAACAATTATATACAGCAGTAGCTAAATTCTTCGAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1243

Amino Acids

138.74

Weight (kDa)

6.22

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 359 - 553 1.1e-34 CHASE domain
HisKA PF00512 641 - 706 9.3e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 848 - 919 2.4e-17 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 942 - 1080 1.9e-37 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 1104 - 1236 1.4e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2445
Acc16I TGCGCA 1 cut(s) 2049
Acc36I ACCTGC 2 cut(s) 52, 1734
AccB7I CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3284
AccI GTMKAC 2 cut(s) 542, 2223
AccII CGCG 1 cut(s) 1371
AccIII TCCGGA 1 cut(s) 1959
AciI CCGC 5 cut(s) 1369, 1838, 2357, 3246, 3399
AclI AACGTT 2 cut(s) 2494, 2683
AclWI GGATC 4 cut(s) 592, 1265, 1278, 1709
AcoI YGGCCR 3 cut(s) 1839, 1920, 2994
AcsI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2577, 2828, 3501, 3713
AcuI CTGAAG 2 cut(s) 2265, 2898
AcvI CACGTG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 2269
AfaI GTAC 4 cut(s) 1410, 2139, 2743, 2965
AfiI CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
AflII CTTAAG 1 cut(s) 3422
AflIII ACRYGT 3 cut(s) 963, 1653, 2987
AhdI GACNNNNNGTC 1 cut(s) 1351
AhlI ACTAGT 1 cut(s) 1136
AjnI CCWGG 8 cut(s) 78, 165, 462, 485, 1446, 2263, 2728, 3120
AleI CACNNNNGTG 1 cut(s) 611
AloI GAACNNNNNNTCC 2 cut(s) 2767, 2799
Alw21I GWGCWC 3 cut(s) 608, 2848, 3069
Alw44I GTGCAC 1 cut(s) 604
AlwI GGATC 4 cut(s) 592, 1265, 1278, 1709
AlwNI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
Aor13HI TCCGGA 1 cut(s) 1959
AoxI GGCC 9 cut(s) 86, 1839, 1920, 2100, 2116, 2622, 2801, 2994, 3234
ApaLI GTGCAC 1 cut(s) 604
ApoI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2577, 2828, 3501, 3713
ArsI GACNNNNNNTTYG 4 cut(s) 2673, 2705, 3493, 3525
AseI ATTAAT 1 cut(s) 2546
AspLEI GCGC 2 cut(s) 1300, 2050
AspS9I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
AsuC2I CCSGG 1 cut(s) 982
AsuHPI GGTGA 7 cut(s) 1046, 1109, 1436, 1724, 2308, 2860, 3376
AsuII TTCGAA 1 cut(s) 3486
AvaII GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 2 cut(s) 608, 749
BaeI ACNNNNGTAYC 2 cut(s) 2556, 2589
BalI TGGCCA 2 cut(s) 1922, 2996
BamHI GGATCC 1 cut(s) 1270
BanII GRGCYC 2 cut(s) 78, 982
BbrPI CACGTG 1 cut(s) 849
BbsI GAAGAC 2 cut(s) 209, 2783
Bbv12I GWGCWC 3 cut(s) 608, 2848, 3069
BciT130I CCWGG 8 cut(s) 80, 167, 464, 487, 1448, 2265, 2730, 3122
BciVI GTATCC 1 cut(s) 2557
BclI TGATCA 3 cut(s) 1081, 1647, 2095
BcnI CCSGG 1 cut(s) 982
BcuI ACTAGT 1 cut(s) 1136
BfaI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2585, 2640, 2789, 2961
BfmI CTRYAG 5 cut(s) 509, 543, 812, 1741, 1911
BfrI CTTAAG 1 cut(s) 3422
BfuAI ACCTGC 2 cut(s) 52, 1734
BfuI GTATCC 1 cut(s) 2557
BglII AGATCT 1 cut(s) 2065
BmcAI AGTACT 1 cut(s) 1410
Bme1390I CCNGG 9 cut(s) 80, 167, 464, 487, 982, 1448, 2265, 2730, 3122
Bme18I GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
BmeRI GACNNNNNGTC 1 cut(s) 1351
BmgT120I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
BmiI GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
BmrFI CCNGG 9 cut(s) 80, 167, 464, 487, 982, 1448, 2265, 2730, 3122
BmrI ACTGGG 2 cut(s) 1689, 2766
BmuI ACTGGG 2 cut(s) 1689, 2766
BoxI GACNNNNGTC 1 cut(s) 1241
BpiI GAAGAC 2 cut(s) 209, 2783
BpmI CTGGAG 3 cut(s) 192, 1476, 3533
Bpu14I TTCGAA 1 cut(s) 3486
BpuEI CTTGAG 1 cut(s) 1299
BpuMI CCSGG 1 cut(s) 982
BsaAI YACGTR 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 2269
BsaI GGTCTC 3 cut(s) 1120, 2253, 2418
BsaJI CCNNGG 9 cut(s) 78, 166, 450, 485, 1057, 1447, 1769, 2263, 2997
BsaWI WCCGGW 1 cut(s) 1959
BsaXI ACNNNNNCTCC 6 cut(s) 1788, 1818, 2699, 2729, 3366, 3396
Bsc4I CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
Bse118I RCCGGY 1 cut(s) 2118
Bse1I ACTGG 7 cut(s) 1569, 1684, 2662, 2761, 2853, 2931, 3040
Bse21I CCTNAGG 1 cut(s) 834
Bse3DI GCAATG 2 cut(s) 75, 3577
BseAI TCCGGA 1 cut(s) 1959
BseBI CCWGG 8 cut(s) 80, 167, 464, 487, 1448, 2265, 2730, 3122
BseDI CCNNGG 9 cut(s) 78, 166, 450, 485, 1057, 1447, 1769, 2263, 2997
BseLI CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
BseMI GCAATG 2 cut(s) 75, 3577
BseMII CTCAG 9 cut(s) 635, 1164, 1218, 2031, 2199, 2759, 2819, 2831, 2838
BseNI ACTGG 7 cut(s) 1569, 1684, 2662, 2761, 2853, 2931, 3040
BseSI GKGCMC 2 cut(s) 608, 749
BsgI GTGCAG 3 cut(s) 1565, 1806, 1923
Bsh1236I CGCG 1 cut(s) 1371
BshFI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
BsiHKAI GWGCWC 3 cut(s) 608, 2848, 3069
BsiSI CCGG 3 cut(s) 982, 1960, 2119
BslFI GGGAC 3 cut(s) 195, 1541, 2225
BslI CCNNNNNNNGG 8 cut(s) 83, 84, 274, 463, 1564, 1973, 2735, 3284
BsmBI CGTCTC 2 cut(s) 1048, 2233
BsmFI GGGAC 3 cut(s) 195, 1541, 2225
BsnI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
Bso31I GGTCTC 3 cut(s) 1120, 2253, 2418
Bsp119I TTCGAA 1 cut(s) 3486
Bsp1286I GDGCHC 6 cut(s) 78, 608, 749, 982, 2848, 3069
Bsp13I TCCGGA 1 cut(s) 1959
Bsp19I CCATGG 2 cut(s) 1057, 2997
BspACI CCGC 5 cut(s) 1369, 1838, 2357, 3246, 3399
BspANI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
BspCNI CTCAG 9 cut(s) 634, 1165, 1219, 2032, 2198, 2760, 2818, 2832, 2837
BspEI TCCGGA 1 cut(s) 1959
BspFNI CGCG 1 cut(s) 1371
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
BspMAI CTGCAG 2 cut(s) 1745, 1915
BspMI ACCTGC 2 cut(s) 52, 1734
BspPI GGATC 4 cut(s) 592, 1265, 1278, 1709
BspT104I TTCGAA 1 cut(s) 3486
BspTI CTTAAG 1 cut(s) 3422
BspTNI GGTCTC 3 cut(s) 1120, 2253, 2418
BsrDI GCAATG 2 cut(s) 75, 3577
BsrFI RCCGGY 1 cut(s) 2118
BsrI ACTGG 7 cut(s) 1569, 1684, 2662, 2761, 2853, 2931, 3040
BssAI RCCGGY 1 cut(s) 2118
BssECI CCNNGG 9 cut(s) 78, 166, 450, 485, 1057, 1447, 1769, 2263, 2997
BssNI GRCGYC 1 cut(s) 2269
BssT1I CCWWGG 4 cut(s) 450, 1057, 1769, 2997
Bst2UI CCWGG 8 cut(s) 80, 167, 464, 487, 1448, 2265, 2730, 3122
Bst6I CTCTTC 3 cut(s) 2056, 3204, 3337
BstACI GRCGYC 1 cut(s) 2269
BstAFI CTTAAG 1 cut(s) 3422
BstAPI GCANNNNNTGC 3 cut(s) 1784, 1910, 3356
BstBAI YACGTR 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 3486
BstDSI CCRYGG 2 cut(s) 1057, 2997
BstFNI CGCG 1 cut(s) 1371
BstHHI GCGC 2 cut(s) 1300, 2050
BstNI CCWGG 8 cut(s) 80, 167, 464, 487, 1448, 2265, 2730, 3122
BstNSI RCATGY 6 cut(s) 967, 1657, 2054, 2949, 2991, 3421
BstPAI GACNNNNGTC 1 cut(s) 1241
BstSCI CCNGG 9 cut(s) 78, 165, 462, 485, 980, 1446, 2263, 2728, 3120
BstSFI CTRYAG 5 cut(s) 509, 543, 812, 1741, 1911
BstSLI GKGCMC 2 cut(s) 608, 749
BstUI CGCG 1 cut(s) 1371
BstV2I GAAGAC 2 cut(s) 209, 2783
BstX2I RGATCY 2 cut(s) 1270, 2065
BstXI CCANNNNNNTGG 3 cut(s) 692, 1447, 2992
BstYI RGATCY 2 cut(s) 1270, 2065
Bsu36I CCTNAGG 1 cut(s) 834
BsuI GTATCC 1 cut(s) 2557
BsuRI GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
BtgI CCRYGG 2 cut(s) 1057, 2997
BtsI GCAGTG 2 cut(s) 1738, 1797
BtsIMutI CAGTG 7 cut(s) 510, 1562, 1738, 1797, 2163, 2655, 2846
BveI ACCTGC 2 cut(s) 52, 1734
CaiI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
CciI TCATGA 1 cut(s) 739
CfoI GCGC 2 cut(s) 1300, 2050
Cfr10I RCCGGY 1 cut(s) 2118
Cfr13I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
CseI GACGC 2 cut(s) 2277, 3452
Csp6I GTAC 4 cut(s) 1409, 2138, 2742, 2964
CviQI GTAC 4 cut(s) 1409, 2138, 2742, 2964
DraI TTTAAA 3 cut(s) 1158, 2500, 3363
DriI GACNNNNNGTC 1 cut(s) 1351
EaeI YGGCCR 3 cut(s) 1839, 1920, 2994
Eam1104I CTCTTC 3 cut(s) 2056, 3204, 3337
Eam1105I GACNNNNNGTC 1 cut(s) 1351
EarI CTCTTC 3 cut(s) 2056, 3204, 3337
EciI GGCGGA 1 cut(s) 2372
Eco130I CCWWGG 4 cut(s) 450, 1057, 1769, 2997
Eco24I GRGCYC 2 cut(s) 78, 982
Eco31I GGTCTC 3 cut(s) 1120, 2253, 2418
Eco32I GATATC 1 cut(s) 2891
Eco47I GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
Eco57I CTGAAG 2 cut(s) 2265, 2898
Eco72I CACGTG 1 cut(s) 849
Eco81I CCTNAGG 1 cut(s) 834
EcoO109I RGGNCCY 1 cut(s) 3235
EcoRI GAATTC 3 cut(s) 2302, 2577, 3501
EcoRII CCWGG 8 cut(s) 78, 165, 462, 485, 1446, 2263, 2728, 3120
EcoRV GATATC 1 cut(s) 2891
EcoT14I CCWWGG 4 cut(s) 450, 1057, 1769, 2997
EcoT22I ATGCAT 1 cut(s) 1639
EcoT38I GRGCYC 2 cut(s) 78, 982
ErhI CCWWGG 4 cut(s) 450, 1057, 1769, 2997
Esp3I CGTCTC 2 cut(s) 1048, 2233
FalI AAGNNNNNCTT 6 cut(s) 939, 971, 1239, 1271, 3222, 3254
FaqI GGGAC 3 cut(s) 195, 1541, 2225
FauNDI CATATG 1 cut(s) 2895
FbaI TGATCA 3 cut(s) 1081, 1647, 2095
FblI GTMKAC 2 cut(s) 542, 2223
FriOI GRGCYC 2 cut(s) 78, 982
FspBI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2585, 2640, 2789, 2961
FspI TGCGCA 1 cut(s) 2049
GlaI GCGC 2 cut(s) 1299, 2049
GsuI CTGGAG 3 cut(s) 192, 1476, 3533
HaeIII GGCC 9 cut(s) 88, 1841, 1922, 2102, 2118, 2624, 2803, 2996, 3236
HapII CCGG 3 cut(s) 982, 1960, 2119
HgaI GACGC 2 cut(s) 2277, 3452
HhaI GCGC 2 cut(s) 1300, 2050
Hin1I GRCGYC 1 cut(s) 2269
Hin6I GCGC 2 cut(s) 1298, 2048
HinP1I GCGC 2 cut(s) 1298, 2048
HincII GTYRAC 2 cut(s) 1395, 3337
HindII GTYRAC 2 cut(s) 1395, 3337
HindIII AAGCTT 2 cut(s) 116, 1264
HpaII CCGG 3 cut(s) 982, 1960, 2119
HphI GGTGA 7 cut(s) 1046, 1109, 1436, 1724, 2308, 2860, 3376
Hpy99I CGWCG 1 cut(s) 3501
HpyAV CCTTC 7 cut(s) 376, 565, 1080, 1186, 2282, 2362, 3347
HpyCH4IV ACGT 7 cut(s) 848, 896, 2226, 2494, 2683, 3044, 3148
HpySE526I ACGT 7 cut(s) 848, 896, 2226, 2494, 2683, 3044, 3148
Hsp92I GRCGYC 1 cut(s) 2269
HspAI GCGC 2 cut(s) 1298, 2048
Kpn2I TCCGGA 1 cut(s) 1959
KroI GCCGGC 1 cut(s) 2118
KroNI GCCGGC 1 cut(s) 2120
Ksp22I TGATCA 3 cut(s) 1081, 1647, 2095
LmnI GCTCC 3 cut(s) 73, 1581, 3374
MaeI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2585, 2640, 2789, 2961
MaeII ACGT 7 cut(s) 848, 896, 2226, 2494, 2683, 3044, 3148
MaeIII GTNAC 7 cut(s) 326, 1162, 2551, 2881, 3040, 3291, 3382
MfeI CAATTG 3 cut(s) 686, 1605, 3378
MflI RGATCY 2 cut(s) 1270, 2065
MhlI GDGCHC 6 cut(s) 78, 608, 749, 982, 2848, 3069
MlsI TGGCCA 2 cut(s) 1922, 2996
MluNI TGGCCA 2 cut(s) 1922, 2996
MlyI GAGTC 6 cut(s) 199, 1231, 1806, 3098, 3354, 3731
MmeI TCCRAC 3 cut(s) 646, 984, 2401
Mox20I TGGCCA 2 cut(s) 1922, 2996
Mph1103I ATGCAT 1 cut(s) 1639
MroI TCCGGA 1 cut(s) 1959
MroNI GCCGGC 1 cut(s) 2118
MscI TGGCCA 2 cut(s) 1922, 2996
MslI CAYNNNNRTG 7 cut(s) 252, 611, 921, 1023, 1346, 2055, 2519
Msp20I TGGCCA 2 cut(s) 1922, 2996
MspA1I CMGCKG 2 cut(s) 2819, 3611
MspCI CTTAAG 1 cut(s) 3422
MspI CCGG 3 cut(s) 982, 1960, 2119
MspR9I CCNGG 9 cut(s) 80, 167, 464, 487, 982, 1448, 2265, 2730, 3122
MunI CAATTG 3 cut(s) 686, 1605, 3378
MvaI CCWGG 8 cut(s) 80, 167, 464, 487, 1448, 2265, 2730, 3122
MvnI CGCG 1 cut(s) 1371
NaeI GCCGGC 1 cut(s) 2120
NciI CCSGG 1 cut(s) 982
NcoI CCATGG 2 cut(s) 1057, 2997
NdeI CATATG 1 cut(s) 2895
NgoMIV GCCGGC 1 cut(s) 2118
NlaIV GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
NmuCI GTSAC 2 cut(s) 2881, 3382
NsbI TGCGCA 1 cut(s) 2049
NsiI ATGCAT 1 cut(s) 1639
NspI RCATGY 6 cut(s) 967, 1657, 2054, 2949, 2991, 3421
NspV TTCGAA 1 cut(s) 3486
OliI CACNNNNGTG 1 cut(s) 611
PaeI GCATGC 2 cut(s) 2054, 2949
PagI TCATGA 1 cut(s) 739
PciI ACATGT 3 cut(s) 963, 1653, 2987
PdiI GCCGGC 1 cut(s) 2120
PfeI GAWTC 9 cut(s) 234, 299, 617, 1015, 2111, 2865, 2939, 3483, 3619
PflMI CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3284
PleI GAGTC 6 cut(s) 199, 1231, 1805, 3097, 3353, 3730
PmaCI CACGTG 1 cut(s) 849
PmlI CACGTG 1 cut(s) 849
PpsI GAGTC 6 cut(s) 199, 1231, 1805, 3097, 3353, 3730
Ppu21I YACGTR 1 cut(s) 849
PscI ACATGT 3 cut(s) 963, 1653, 2987
PshAI GACNNNNGTC 1 cut(s) 1241
PshBI ATTAAT 1 cut(s) 2546
PsiI TTATAA 1 cut(s) 2445
Psp1406I AACGTT 2 cut(s) 2494, 2683
Psp6I CCWGG 8 cut(s) 78, 165, 462, 485, 1446, 2263, 2728, 3120
PspCI CACGTG 1 cut(s) 849
PspGI CCWGG 8 cut(s) 78, 165, 462, 485, 1446, 2263, 2728, 3120
PspN4I GGNNCC 6 cut(s) 75, 719, 1272, 2241, 2373, 2529
PspPI GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
PstI CTGCAG 2 cut(s) 1745, 1915
PstNI CAGNNNCTG 4 cut(s) 274, 551, 733, 1910
PsuI RGATCY 2 cut(s) 1270, 2065
PvuII CAGCTG 2 cut(s) 2819, 3611
RsaI GTAC 4 cut(s) 1410, 2139, 2743, 2965
RsaNI GTAC 4 cut(s) 1409, 2138, 2742, 2964
RseI CAYNNNNRTG 7 cut(s) 252, 611, 921, 1023, 1346, 2055, 2519
Sau96I GGNCC 7 cut(s) 86, 454, 1231, 2239, 2372, 3235, 3457
ScaI AGTACT 1 cut(s) 1410
SchI GAGTC 6 cut(s) 199, 1231, 1806, 3098, 3354, 3731
ScrFI CCNGG 9 cut(s) 80, 167, 464, 487, 982, 1448, 2265, 2730, 3122
SduI GDGCHC 6 cut(s) 78, 608, 749, 982, 2848, 3069
SfcI CTRYAG 5 cut(s) 509, 543, 812, 1741, 1911
SfuI TTCGAA 1 cut(s) 3486
SinI GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
SmiMI CAYNNNNRTG 7 cut(s) 252, 611, 921, 1023, 1346, 2055, 2519
SmlI CTYRAG 2 cut(s) 1314, 3422
SmoI CTYRAG 2 cut(s) 1314, 3422
SpeI ACTAGT 1 cut(s) 1136
SphI GCATGC 2 cut(s) 2054, 2949
SsiI CCGC 5 cut(s) 1369, 1838, 2357, 3246, 3399
SspMI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2585, 2640, 2789, 2961
StyD4I CCNGG 9 cut(s) 78, 165, 462, 485, 980, 1446, 2263, 2728, 3120
StyI CCWWGG 4 cut(s) 450, 1057, 1769, 2997
TaiI ACGT 7 cut(s) 851, 899, 2229, 2497, 2686, 3047, 3151
TatI WGTACW 1 cut(s) 1408
TauI GCSGC 1 cut(s) 1841
TfiI GAWTC 9 cut(s) 234, 299, 617, 1015, 2111, 2865, 2939, 3483, 3619
TscAI CASTG 7 cut(s) 510, 1569, 1745, 1797, 2163, 2662, 2853
TseFI GTSAC 2 cut(s) 2881, 3382
Tsp45I GTSAC 2 cut(s) 2881, 3382
TspRI CASTG 7 cut(s) 510, 1569, 1745, 1797, 2163, 2662, 2853
Van91I CCANNNNNTGG 4 cut(s) 274, 463, 1973, 3284
Vha464I CTTAAG 1 cut(s) 3422
VneI GTGCAC 1 cut(s) 604
VpaK11BI GGWCC 5 cut(s) 454, 1231, 2239, 2372, 3457
VspI ATTAAT 1 cut(s) 2546
XapI RAATTY 8 cut(s) 407, 561, 2302, 2413, 2577, 2828, 3501, 3713
XbaI TCTAGA 2 cut(s) 2639, 2788
XceI RCATGY 6 cut(s) 967, 1657, 2054, 2949, 2991, 3421
XmiI GTMKAC 2 cut(s) 542, 2223
XspI CTAG 9 cut(s) 1137, 1538, 1802, 1817, 2055, 2585, 2640, 2789, 2961
ZrmI AGTACT 1 cut(s) 1410
Zsp2I ATGCAT 1 cut(s) 1639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.