MD09G1121900.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003045
Physical Location & Seq
Reverse (-)
9395362 .. 9403547
8186 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1121900.v1.1.491

Sequence Viewer

Length: 3684 bp
ATGTATCTGAATTGTAGGGTATCCTCTGGTTTTAATGGCAGATTGCCCTTCAATCTTGACTTGAACAAGGCAATGGAGACTTTGCATGGGACTAGCTGTACCAGAAGATGGAGAGGAAAGGTCCTAATTCTGGGGCTTCTGGGTTTTGTTACAATCATTTGGGGTTTCTTTAGTTTCAATGATGGAGGTTTGGGGATGAGAGAGAAGGCTCCAGATATGGGTGGAGAGAAAGCTTGGATCCTGCAGCAGCATTTCAATGTCAGCAAATCCCAACTTCTCGCGTTCGCTTCGTTGTTCTCCGAATCAGATCAGATAGCATCCCTTGAATGTACCAAAGAACCAGGACCAGGTATGATGTTAGGTAATGTCATTTCCTGTTATCTTAAGGTGCTATGTTCAGATGAGGAGTTTCAGAAGCGACATAAATGGGCAGCAGAAAATGAAGAAGCCAAGGACCAATGCCTGGTTCAAGATGAGAACATCCGCAGGAAGTGTGATGTATCATTGCTGGAGGACACATCCCCATCATGCACTGTGCAATCTACAGTTTCAGCTAATAGGATTTGTGAAAAGGAGGAGTGTGCAAAAGAGAACTCTCGGATGCTTTGTACTTTGGTAATACAATGTTGGTGGGCATTTGTTGGAATGATCATGTGTTACAAACTGTCTGGTTTTTCTATGAAATTGTGGAGGAATAAAAAACAGAAGTTGGTTCAAAAGGTTGAAGAGCAGCCATTGCGTCGGAGGAAGCAGCCAGAGAAACAGCAACAGCAACCCCCAAAGCCTCCTAAGGTTGCTGGCACTTGGAGGAAGAATCTACTTTTAGTATTTATCGTATTTGGTTTCATTACATCATTGTGGTTATGGTGGCACTTGTGTGCAAGAGATCATTTGTGGAGGGAAGAAACACTTGCCAACATGTGCGATGAACGAGCCCGGATGTTGCAGGATCAGTTTAATGTGAGCCTGAATCATGTTCATGCTTTAGCTATTCTGGTCTCCACCTTTCACCATGGGAAACAACCTACGGCAATTGATCAGAAAACATTTGGTGAATATACAGAGCGAACAGCTTTTGAGAGACCCCTTACGAGTGGTGTTGCTTATGCTTTGAAAGTTACTCATGCCGAGAGGGAACAATTTGAGAAGGAACATGGATGGACAATAAAAAAAATGGAAACTGAGGACCAGACTCTAGTCCAAGATTTTATTACAGAAAGCTTGGCCCCTGCGCCTGTTCAAGATGAATATGCACCCGTGATATTTTCTCAAGAAACTGTCTCCCACATTGTCTCTATTGACATGATGTCCGGAAAGGAAGACCGTGAGAACATCTTGCGAGCAAGGGCAACTGGAAAGGGAGTATTGACCTCCCCGTTTAAGCTATTGAAATCCAATCACCTGGGAGTTGTACTCACATTCGCCGTCTATAATGCTGATTTGCCTCCACATTCCACACCAGAGCAACGAATTCAAGCTACTGTGGGGTATCTAGGTGCATCATATGATGTACCGTCACTGGTGGAGAAGCTCCTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATTCATCTGCTCCGATCAACATGTATGGTACTGATGTTATTGATACTGGCCTGTTGCAAATTAGCAACCTGGATTTTGGGGATCCACAACGCAAACATGAAATGCACTGCAGGTTTAAGCACAAACCTCCGTTTCCTTTGACAGCAGCAAGTGCATCCATAGGAGTCCTAGTCATTACCTTCCTAGTTAGTCATATTTTTCATGCGGCCATAAGCCGAATAGCAAAAGTGGAGGCAGACTATCGTGAGATGATGGAACTCAAAGTTCGTGCAGAAGCTGCAGATGTGGCCAAATCTCAGTTCCTTGCAACTGTTTCCCATGAGATCAGGACACCAATGAATGGTGTTTTAGGCATGCTGCAAATGCTGATGGACACCGATCTAGGTCCAAACCAACAGGACTACGCGGAGACTGCTCATGCCAGCGGGAGAGAACTGATATCGCTGATAAATGAGGTTCTTGATCAGGCCAAAATCGAATCAGGCAGGCTGGAACTTGAAACTGTTCCTTTTGATCTGCGTTCAGTGCTTGATAATGTTCTATCACTTAACTCAGGAAAATCCAACGAGAAAGGGATCGAGTTGGCTGCTTATGTCTCCAATATGGTACCTGAAGTTGTTATTGGTGATCCTGGACGCTTTCGGCAGATAATCACAAATCTTGTTGGGAATTCAATTAAGTTCACCCATGACAAAGGACACATATTTGTCTCGGTGCATCTGGTGGATGAAGTGAGGGCCCCACCTGATCTTATGGATGAAGTTCTAAGACAAGGCTTTAACCTAGTTGGAGATGTCTCAAACAAAACTTATAATACATTGAGTGGCTTTCCTGTTGTCAATAGATGGAAAAGCTGGGAAGGTTTTAATTCAATACGCAGCACAACACTGGAGAAATCTGATATGATCAAATTACTCGTAACAGTTGAGGATACAGGTGTAGGAATCCCTCTAGATGCACAAAATCGCATCTTCACACCTTTTATGCAGGCTGATAGCTCCACTTCTAGAACTTATGGTGGTACTGGAATAGGATTGAGCATTAGCAAACGTCTGGTAGATCTCATGAGTGGGGAGATAGGGTTTGTAAGTGAACCTGGCACCGGAAGTACCTTTTCATTTATGGGAACTTTTGGGAAGGGAGAATTAAGTTCCCTGGAGACAAAGTGGCACCAGTATGAACCAGCTGTCTCAGAGTTTCAAGGACTGAGAGCATTGGTAATAGATAATAAAACCATCCGAACTGAGGTCACGAAATACCATATGCAGAGATTGGGAATATCTGTGGATACAGCGTCCTGTCTGGAATCAGCGTGCTCTTATTTATCTAGCGCTGGCAACACCAGTCTTTCCACACCGTTGGCCATGGTTCTTATTGACAAAGATGTTTGGGATAAAGAAACTGGTATTATATTGCATCAGTCACTTAAAGAGCACAGGAAAAACAACGTGGAATTGCTCATAAACCTTCCGAAGATACTTCTCGTGGCTACTTCCATCACTTCTGTTGAACGCAATGTAGTAACATCTTTAGGTCTTGTAGATAAAGTATTGACAAAGCCACTTCGGTTAAGTGTCTTAATTGCTTGCTTTCAAGAAGCCCTTGGAAGACGTAAGAAGAGGCTTCTAAATGCAAACAAGCCAACTCTTGGAAAGTTACTAAGAGAGAAGAGAATTTTGGTGGTGGATGACAATGTAGTGAACAGAAGAGTGGCAGAAGGTGCTTTAAAGAAGTATGGAGCAATCGTCACCTGTGTGGAGAGCGGAAAGGTTGCTTTGTTGAAGCTTAAACCACCCCACAACTTTGATGCTTGCTTTATGGATCTCCAGATGCCGGAAATGGATGGGTTTGAAGCAACTCGGATCATCCGTGGTATGGAAAGTGAGGTAAATGAGAAAATTGCAGCTGGGGAAGCATCAGTTGAGATGTTTGGCAATGTCGTAAATTGGCACACGCCAATATTGGCTATGACGGCTGATGTGATTCAGGCTTCAAATGAAGAGTGCATAAAATGCGGGATGGATGATTATGTGTCAAAGCCATTTGAAGAAGACCAGCTGTATTCAGCAGTGGCACGTTTCTTTGAGTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1228

Amino Acids

137.24

Weight (kDa)

6.43

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 344 - 538 1.1e-36 CHASE domain
HisKA PF00512 626 - 691 7.8e-20 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 832 - 903 8.9e-18 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 927 - 1065 5.2e-35 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 1088 - 1220 3.1e-18 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2400
Acc36I ACCTGC 1 cut(s) 1689
Acc65I GGTACC 1 cut(s) 2194
AccB1I GGYRCC 3 cut(s) 2194, 2687, 2757
AccB7I CCANNNNNTGG 4 cut(s) 108, 463, 1928, 3236
AccBSI CCGCTC 1 cut(s) 3351
AccII CGCG 2 cut(s) 281, 1994
AccIII TCCGGA 1 cut(s) 1310
AciI CCGC 6 cut(s) 484, 1793, 1994, 2013, 3351, 3603
AclWI GGATC 9 cut(s) 232, 245, 957, 1664, 1677, 2171, 2210, 3417, 3458
AcoI YGGCCR 3 cut(s) 1794, 1875, 2949
AcsI RAATTY 3 cut(s) 1470, 2257, 3261
AcuI CTGAAG 1 cut(s) 2220
AfaI GTAC 9 cut(s) 100, 331, 610, 1413, 1512, 1618, 2196, 2611, 2698
AfeI AGCGCT 1 cut(s) 2920
AflII CTTAAG 1 cut(s) 383
AflIII ACRYGT 2 cut(s) 918, 1608
AhdI GACNNNNNGTC 1 cut(s) 1306
AjnI CCWGG 8 cut(s) 340, 346, 462, 1401, 1656, 2218, 2683, 2742
AjuI GAANNNNNNNTTGG 8 cut(s) 450, 482, 1871, 1903, 2193, 2225, 3248, 3280
AleI CACNNNNGTG 2 cut(s) 876, 3341
Alw21I GWGCWC 2 cut(s) 2906, 3024
AlwI GGATC 9 cut(s) 232, 245, 957, 1664, 1677, 2171, 2210, 3417, 3458
AlwNI CAGNNNCTG 1 cut(s) 1865
Aor13HI TCCGGA 1 cut(s) 1310
Aor51HI AGCGCT 1 cut(s) 2920
AoxI GGCC 7 cut(s) 1224, 1636, 1794, 1875, 2055, 2325, 2949
ApaI GGGCCC 1 cut(s) 2329
ApoI RAATTY 3 cut(s) 1470, 2257, 3261
Asp700I GAANNNNTTC 1 cut(s) 2091
Asp718I GGTACC 1 cut(s) 2194
AspLEI GCGC 2 cut(s) 1234, 2921
AspS9I GGNCC 8 cut(s) 121, 344, 454, 1186, 1225, 1973, 2325, 2326
AsuC2I CCSGG 1 cut(s) 937
AsuHPI GGTGA 6 cut(s) 1001, 1064, 1391, 2225, 2263, 3328
AvaII GGWCC 5 cut(s) 121, 344, 454, 1186, 1973
AxyI CCTNAGG 1 cut(s) 789
BaeGI GKGCMC 1 cut(s) 2329
BaeI ACNNNNGTAYC 4 cut(s) 1608, 1641, 2511, 2544
BalI TGGCCA 2 cut(s) 1877, 2951
BamHI GGATCC 2 cut(s) 237, 1669
BanI GGYRCC 3 cut(s) 2194, 2687, 2757
BanII GRGCYC 2 cut(s) 937, 2329
BauI CACGAG 1 cut(s) 3071
BbsI GAAGAC 3 cut(s) 1326, 3202, 3645
Bbv12I GWGCWC 2 cut(s) 2906, 3024
BceAI ACGGC 3 cut(s) 1044, 1409, 3576
BcgI CGANNNNNNTGC 2 cut(s) 2156, 2190
BciT130I CCWGG 8 cut(s) 342, 348, 464, 1403, 1658, 2220, 2685, 2744
BciVI GTATCC 3 cut(s) 31, 2512, 2869
BclI TGATCA 4 cut(s) 648, 1036, 2050, 2493
BcnI CCSGG 1 cut(s) 937
BfmI CTRYAG 4 cut(s) 242, 543, 1696, 1866
BfoI RGCGCY 1 cut(s) 2922
BfrI CTTAAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 1689
BfuI GTATCC 3 cut(s) 31, 2512, 2869
BglII AGATCT 1 cut(s) 2647
Bme1390I CCNGG 9 cut(s) 342, 348, 464, 937, 1403, 1658, 2220, 2685, 2744
Bme18I GGWCC 5 cut(s) 121, 344, 454, 1186, 1973
BmeRI GACNNNNNGTC 1 cut(s) 1306
BmgT120I GGNCC 8 cut(s) 121, 344, 454, 1186, 1225, 1973, 2325, 2326
BmiI GGNNCC 9 cut(s) 210, 239, 1227, 1671, 2196, 2327, 2328, 2689, 2759
BmrFI CCNGG 9 cut(s) 342, 348, 464, 937, 1403, 1658, 2220, 2685, 2744
BoxI GACNNNNGTC 1 cut(s) 1196
BpiI GAAGAC 3 cut(s) 1326, 3202, 3645
BplI GAGNNNNNCTC 2 cut(s) 1398, 1430
BpmI CTGGAG 5 cut(s) 195, 530, 2498, 2765, 3398
BpuEI CTTGAG 1 cut(s) 1254
BpuMI CCSGG 1 cut(s) 937
BsaI GGTCTC 2 cut(s) 1003, 1075
BsaJI CCNNGG 7 cut(s) 450, 1012, 1402, 2742, 2952, 3190, 3457
BsaWI WCCGGW 2 cut(s) 1310, 2690
BsaXI ACNNNNNCTCC 8 cut(s) 1743, 1773, 2654, 2684, 2738, 2768, 3318, 3348
Bse1I ACTGG 8 cut(s) 1357, 1524, 1639, 2481, 2617, 2761, 2931, 2995
Bse21I CCTNAGG 1 cut(s) 789
Bse3DI GCAATG 5 cut(s) 78, 503, 734, 3109, 3529
BseAI TCCGGA 1 cut(s) 1310
BseBI CCWGG 8 cut(s) 342, 348, 464, 1403, 1658, 2220, 2685, 2744
BseDI CCNNGG 7 cut(s) 450, 1012, 1402, 2742, 2952, 3190, 3457
BseMI GCAATG 5 cut(s) 78, 503, 734, 3109, 3529
BseMII CTCAG 6 cut(s) 1173, 1898, 2154, 2786, 2793, 2823
BseNI ACTGG 8 cut(s) 1357, 1524, 1639, 2481, 2617, 2761, 2931, 2995
BseRI GAGGAG 2 cut(s) 419, 590
BseSI GKGCMC 1 cut(s) 2329
BseYI CCCAGC 2 cut(s) 2442, 3494
BsgI GTGCAG 2 cut(s) 1520, 1878
Bsh1236I CGCG 2 cut(s) 281, 1994
BshFI GGCC 7 cut(s) 1226, 1638, 1796, 1877, 2057, 2327, 2951
BshNI GGYRCC 3 cut(s) 2194, 2687, 2757
BsiHKAI GWGCWC 2 cut(s) 2906, 3024
BsiSI CCGG 4 cut(s) 937, 1311, 2691, 3422
BslFI GGGAC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 103
BsnI GGCC 7 cut(s) 1226, 1638, 1796, 1877, 2057, 2327, 2951
Bso31I GGTCTC 2 cut(s) 1003, 1075
Bsp120I GGGCCC 1 cut(s) 2325
Bsp1286I GDGCHC 4 cut(s) 937, 2329, 2906, 3024
Bsp13I TCCGGA 1 cut(s) 1310
Bsp19I CCATGG 2 cut(s) 1012, 2952
BspACI CCGC 6 cut(s) 484, 1793, 1994, 2013, 3351, 3603
BspANI GGCC 7 cut(s) 1226, 1638, 1796, 1877, 2057, 2327, 2951
BspCNI CTCAG 6 cut(s) 1174, 1897, 2153, 2787, 2792, 2824
BspEI TCCGGA 1 cut(s) 1310
BspFNI CGCG 2 cut(s) 281, 1994
BspHI TCATGA 1 cut(s) 2652
BspLI GGNNCC 9 cut(s) 210, 239, 1227, 1671, 2196, 2327, 2328, 2689, 2759
BspMAI CTGCAG 3 cut(s) 246, 1700, 1870
BspMI ACCTGC 1 cut(s) 1689
BspPI GGATC 9 cut(s) 232, 245, 957, 1664, 1677, 2171, 2210, 3417, 3458
BspQI GCTCTTC 1 cut(s) 720
BspT107I GGYRCC 3 cut(s) 2194, 2687, 2757
BspTI CTTAAG 1 cut(s) 383
BspTNI GGTCTC 2 cut(s) 1003, 1075
BsrBI CCGCTC 1 cut(s) 3351
BsrDI GCAATG 5 cut(s) 78, 503, 734, 3109, 3529
BsrI ACTGG 8 cut(s) 1357, 1524, 1639, 2481, 2617, 2761, 2931, 2995
BssECI CCNNGG 7 cut(s) 450, 1012, 1402, 2742, 2952, 3190, 3457
BssSI CACGAG 1 cut(s) 3071
BssT1I CCWWGG 4 cut(s) 450, 1012, 2952, 3190
Bst2BI CACGAG 1 cut(s) 3071
Bst2UI CCWGG 8 cut(s) 342, 348, 464, 1403, 1658, 2220, 2685, 2744
Bst6I CTCTTC 5 cut(s) 720, 3200, 3251, 3289, 3582
BstAFI CTTAAG 1 cut(s) 383
BstAPI GCANNNNNTGC 5 cut(s) 736, 1739, 1865, 3308, 3600
BstDEI CTNAG 9 cut(s) 789, 1182, 1884, 2140, 2354, 2779, 2795, 2832, 3248
BstDSI CCRYGG 3 cut(s) 1012, 2952, 3457
BstFNI CGCG 2 cut(s) 281, 1994
BstH2I RGCGCY 1 cut(s) 2922
BstHHI GCGC 2 cut(s) 1234, 2921
BstNI CCWGG 8 cut(s) 342, 348, 464, 1403, 1658, 2220, 2685, 2744
BstNSI RCATGY 3 cut(s) 922, 1612, 1945
BstPAI GACNNNNGTC 1 cut(s) 1196
BstSCI CCNGG 9 cut(s) 340, 346, 462, 935, 1401, 1656, 2218, 2683, 2742
BstSFI CTRYAG 4 cut(s) 242, 543, 1696, 1866
BstSLI GKGCMC 1 cut(s) 2329
BstUI CGCG 2 cut(s) 281, 1994
BstV2I GAAGAC 3 cut(s) 1326, 3202, 3645
BstX2I RGATCY 4 cut(s) 237, 1669, 2647, 3409
BstXI CCANNNNNNTGG 2 cut(s) 1402, 2947
BstYI RGATCY 4 cut(s) 237, 1669, 2647, 3409
Bsu36I CCTNAGG 1 cut(s) 789
BsuI GTATCC 3 cut(s) 31, 2512, 2869
BsuRI GGCC 7 cut(s) 1226, 1638, 1796, 1877, 2057, 2327, 2951
BtgI CCRYGG 3 cut(s) 1012, 2952, 3457
BtgZI GCGATG 1 cut(s) 939
BtsI GCAGTG 2 cut(s) 1693, 3663
BtsIMutI CAGTG 6 cut(s) 531, 1517, 1693, 2118, 2474, 3663
BveI ACCTGC 1 cut(s) 1689
CaiI CAGNNNCTG 1 cut(s) 1865
CciI TCATGA 1 cut(s) 2652
CfoI GCGC 2 cut(s) 1234, 2921
Cfr13I GGNCC 8 cut(s) 121, 344, 454, 1186, 1225, 1973, 2325, 2326
CseI GACGC 3 cut(s) 728, 2232, 2871
CsiI ACCWGGT 1 cut(s) 346
Csp6I GTAC 9 cut(s) 99, 330, 609, 1412, 1511, 1617, 2195, 2610, 2697
CspCI CAANNNNNGTGG 2 cut(s) 611, 646
CviQI GTAC 9 cut(s) 99, 330, 609, 1412, 1511, 1617, 2195, 2610, 2697
DdeI CTNAG 9 cut(s) 789, 1182, 1884, 2140, 2354, 2779, 2795, 2832, 3248
DraI TTTAAA 1 cut(s) 3315
DriI GACNNNNNGTC 1 cut(s) 1306
EaeI YGGCCR 3 cut(s) 1794, 1875, 2949
Eam1104I CTCTTC 5 cut(s) 720, 3200, 3251, 3289, 3582
Eam1105I GACNNNNNGTC 1 cut(s) 1306
EarI CTCTTC 5 cut(s) 720, 3200, 3251, 3289, 3582
Eco130I CCWWGG 4 cut(s) 450, 1012, 2952, 3190
Eco24I GRGCYC 2 cut(s) 937, 2329
Eco31I GGTCTC 2 cut(s) 1003, 1075
Eco32I GATATC 1 cut(s) 2028
Eco47I GGWCC 5 cut(s) 121, 344, 454, 1186, 1973
Eco47III AGCGCT 1 cut(s) 2920
Eco57I CTGAAG 1 cut(s) 2220
Eco81I CCTNAGG 1 cut(s) 789
EcoO109I RGGNCCY 3 cut(s) 121, 2325, 2326
EcoRI GAATTC 2 cut(s) 1470, 2257
EcoRII CCWGG 8 cut(s) 340, 346, 462, 1401, 1656, 2218, 2683, 2742
EcoRV GATATC 1 cut(s) 2028
EcoT14I CCWWGG 4 cut(s) 450, 1012, 2952, 3190
EcoT38I GRGCYC 2 cut(s) 937, 2329
ErhI CCWWGG 4 cut(s) 450, 1012, 2952, 3190
FalI AAGNNNNNCTT 4 cut(s) 894, 926, 3174, 3206
FaqI GGGAC 1 cut(s) 103
FauI CCCGC 2 cut(s) 2006, 3596
FauNDI CATATG 2 cut(s) 1504, 2850
FbaI TGATCA 4 cut(s) 648, 1036, 2050, 2493
FriOI GRGCYC 2 cut(s) 937, 2329
GlaI GCGC 2 cut(s) 1233, 2920
GsaI CCCAGC 2 cut(s) 2446, 3498
GsuI CTGGAG 5 cut(s) 195, 530, 2498, 2765, 3398
HaeII RGCGCY 1 cut(s) 2922
HaeIII GGCC 7 cut(s) 1226, 1638, 1796, 1877, 2057, 2327, 2951
HapII CCGG 4 cut(s) 937, 1311, 2691, 3422
HgaI GACGC 3 cut(s) 728, 2232, 2871
HhaI GCGC 2 cut(s) 1234, 2921
Hin6I GCGC 2 cut(s) 1232, 2919
HinP1I GCGC 2 cut(s) 1232, 2919
HindIII AAGCTT 3 cut(s) 231, 1219, 3371
HpaII CCGG 4 cut(s) 937, 1311, 2691, 3422
HphI GGTGA 6 cut(s) 1001, 1064, 1391, 2225, 2263, 3328
Hpy166II GTNNAC 3 cut(s) 2271, 2681, 3289
Hpy8I GTNNAC 3 cut(s) 2271, 2681, 3289
Hpy99I CGWCG 1 cut(s) 744
HpyAV CCTTC 8 cut(s) 58, 199, 1141, 1777, 2441, 2719, 3065, 3299
HpyCH4IV ACGT 4 cut(s) 2638, 3036, 3199, 3664
HpyF3I CTNAG 9 cut(s) 789, 1182, 1884, 2140, 2354, 2779, 2795, 2832, 3248
HpySE526I ACGT 4 cut(s) 2638, 3036, 3199, 3664
HspAI GCGC 2 cut(s) 1232, 2919
Kpn2I TCCGGA 1 cut(s) 1310
KpnI GGTACC 1 cut(s) 2198
Ksp22I TGATCA 4 cut(s) 648, 1036, 2050, 2493
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 5 cut(s) 214, 1536, 1603, 2591, 3326
MabI ACCWGGT 1 cut(s) 346
MaeII ACGT 4 cut(s) 2638, 3036, 3199, 3664
MbiI CCGCTC 1 cut(s) 3351
MfeI CAATTG 2 cut(s) 1032, 1560
MflI RGATCY 4 cut(s) 237, 1669, 2647, 3409
MhlI GDGCHC 4 cut(s) 937, 2329, 2906, 3024
MlsI TGGCCA 2 cut(s) 1877, 2951
MluNI TGGCCA 2 cut(s) 1877, 2951
MlyI GAGTC 3 cut(s) 1186, 1761, 3683
MmeI TCCRAC 4 cut(s) 622, 722, 2175, 2356
Mox20I TGGCCA 2 cut(s) 1877, 2951
MroI TCCGGA 1 cut(s) 1310
MroXI GAANNNNTTC 1 cut(s) 2091
MscI TGGCCA 2 cut(s) 1877, 2951
MslI CAYNNNNRTG 6 cut(s) 255, 856, 876, 978, 2763, 3341
Msp20I TGGCCA 2 cut(s) 1877, 2951
MspA1I CMGCKG 4 cut(s) 2013, 2774, 3494, 3646
MspCI CTTAAG 1 cut(s) 383
MspI CCGG 4 cut(s) 937, 1311, 2691, 3422
MspR9I CCNGG 9 cut(s) 342, 348, 464, 937, 1403, 1658, 2220, 2685, 2744
MunI CAATTG 2 cut(s) 1032, 1560
MvaI CCWGG 8 cut(s) 342, 348, 464, 1403, 1658, 2220, 2685, 2744
MvnI CGCG 2 cut(s) 281, 1994
NciI CCSGG 1 cut(s) 937
NcoI CCATGG 2 cut(s) 1012, 2952
NdeI CATATG 2 cut(s) 1504, 2850
NlaIV GGNNCC 9 cut(s) 210, 239, 1227, 1671, 2196, 2327, 2328, 2689, 2759
NmeAIII GCCGAG 1 cut(s) 1153
NmuCI GTSAC 4 cut(s) 1515, 2836, 3009, 3334
NspI RCATGY 3 cut(s) 922, 1612, 1945
OliI CACNNNNGTG 2 cut(s) 876, 3341
PaeI GCATGC 1 cut(s) 1945
PagI TCATGA 1 cut(s) 2652
PciI ACATGT 2 cut(s) 918, 1608
PciSI GCTCTTC 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 3454
PdmI GAANNNNTTC 1 cut(s) 2091
PfeI GAWTC 7 cut(s) 302, 814, 970, 2066, 2532, 2894, 3571
PflMI CCANNNNNTGG 4 cut(s) 108, 463, 1928, 3236
PfoI TCCNGGA 1 cut(s) 2218
PleI GAGTC 3 cut(s) 1186, 1760, 3682
PpsI GAGTC 3 cut(s) 1186, 1760, 3682
PpuMI RGGWCCY 1 cut(s) 121
PscI ACATGT 2 cut(s) 918, 1608
PshAI GACNNNNGTC 1 cut(s) 1196
PsiI TTATAA 1 cut(s) 2400
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 8 cut(s) 340, 346, 462, 1401, 1656, 2218, 2683, 2742
PspFI CCCAGC 2 cut(s) 2442, 3494
PspGI CCWGG 8 cut(s) 340, 346, 462, 1401, 1656, 2218, 2683, 2742
PspN4I GGNNCC 9 cut(s) 210, 239, 1227, 1671, 2196, 2327, 2328, 2689, 2759
PspOMI GGGCCC 1 cut(s) 2325
PspPI GGNCC 8 cut(s) 121, 344, 454, 1186, 1225, 1973, 2325, 2326
PspPPI RGGWCCY 1 cut(s) 121
PstI CTGCAG 3 cut(s) 246, 1700, 1870
PstNI CAGNNNCTG 1 cut(s) 1865
PsuI RGATCY 4 cut(s) 237, 1669, 2647, 3409
PvuII CAGCTG 3 cut(s) 2774, 3494, 3646
RsaI GTAC 9 cut(s) 100, 331, 610, 1413, 1512, 1618, 2196, 2611, 2698
RsaNI GTAC 9 cut(s) 99, 330, 609, 1412, 1511, 1617, 2195, 2610, 2697
RseI CAYNNNNRTG 6 cut(s) 255, 856, 876, 978, 2763, 3341
SapI GCTCTTC 1 cut(s) 720
Sau96I GGNCC 8 cut(s) 121, 344, 454, 1186, 1225, 1973, 2325, 2326
SchI GAGTC 3 cut(s) 1186, 1761, 3683
ScrFI CCNGG 9 cut(s) 342, 348, 464, 937, 1403, 1658, 2220, 2685, 2744
SduI GDGCHC 4 cut(s) 937, 2329, 2906, 3024
SexAI ACCWGGT 1 cut(s) 346
SfcI CTRYAG 4 cut(s) 242, 543, 1696, 1866
SinI GGWCC 5 cut(s) 121, 344, 454, 1186, 1973
SmiMI CAYNNNNRTG 6 cut(s) 255, 856, 876, 978, 2763, 3341
SmlI CTYRAG 2 cut(s) 383, 1269
SmoI CTYRAG 2 cut(s) 383, 1269
SphI GCATGC 1 cut(s) 1945
SsiI CCGC 6 cut(s) 484, 1793, 1994, 2013, 3351, 3603
SspI AATATT 1 cut(s) 3549
StyD4I CCNGG 9 cut(s) 340, 346, 462, 935, 1401, 1656, 2218, 2683, 2742
StyI CCWWGG 4 cut(s) 450, 1012, 2952, 3190
TaiI ACGT 4 cut(s) 2641, 3039, 3202, 3667
TaqI TCGA 2 cut(s) 2064, 2166
TatI WGTACW 2 cut(s) 608, 1411
TauI GCSGC 1 cut(s) 1796
TfiI GAWTC 7 cut(s) 302, 814, 970, 2066, 2532, 2894, 3571
TscAI CASTG 6 cut(s) 538, 1524, 1700, 2118, 2481, 3663
TseFI GTSAC 4 cut(s) 1515, 2836, 3009, 3334
Tsp45I GTSAC 4 cut(s) 1515, 2836, 3009, 3334
TspGWI ACGGA 2 cut(s) 1707, 3446
TspRI CASTG 6 cut(s) 538, 1524, 1700, 2118, 2481, 3663
Van91I CCANNNNNTGG 4 cut(s) 108, 463, 1928, 3236
Vha464I CTTAAG 1 cut(s) 383
VpaK11BI GGWCC 5 cut(s) 121, 344, 454, 1186, 1973
XapI RAATTY 3 cut(s) 1470, 2257, 3261
XbaI TCTAGA 2 cut(s) 2539, 2594
XceI RCATGY 3 cut(s) 922, 1612, 1945
XmnI GAANNNNTTC 1 cut(s) 2091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.