AT5G40190
ERF Family

2'-5' RNA ligase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004409
Physical Location & Seq
Reverse (-)
16069043 .. 16070044
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40190.1

Sequence Viewer

Length: 552 bp
ATGTCAGAAGGCTACGCAATCGAGCTATACTTCGATCCAGCACTCGAAAACCAAGTATTGAAAGCATGGAACGTCTTCGCTCGCCGTCAAATCAGTACCAAATTGATCAACACTGAGTCTCGTCCACACATAACACTCTTCTCCACCTCCTTCTTCGATTCAACGAGACTCGAATCCGTCATCAAAAACTTCGTATCCAAGCAAGAACCAATCTCGATCTCGTTCTCTACTATCGGTAGCTTCTCTAGCGACAACAACGTACTGTTTCTATCTCCGACTCCGTCTCTATCGCTTCTTCAGTTGCAGACTCAGTTATGTGATATGTTGAAGAAAGAATCTGTTGACATTGGTGAAGAGTATCGTGTTGATTCTTGGGTTCCGTTTTGTCCCGTGGCTCTTGATGTTCCTAAGTCTCGTATGGCTGAGGCTTTCTCTGTGTTGAGAGATTTGAAGCTTCCGGTGAATGGTTATGGTATGGAGATTGGTCTCGTTGAGTTTTCTCCTGTTCGTGAAGTCTTCTCATTTCCTCTTGGTAACACTTTGGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2_5_RNA_ligase2

Protein Analysis

183

Amino Acids

20.62

Weight (kDa)

4.86

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2_5_RNA_ligase2 PF13563 39 - 164 1.9e-14 2'-5' RNA ligase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 2 cut(s) 97, 261
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 4 cut(s) 61, 162, 328, 451
AluBI AGCT 3 cut(s) 25, 240, 454
AluI AGCT 3 cut(s) 25, 240, 454
Alw26I GTCTC 5 cut(s) 123, 160, 288, 417, 491
AlwI GGATC 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 362, 472
BbsI GAAGAC 2 cut(s) 67, 508
BbvCI CCTCAGC 1 cut(s) 423
BceAI ACGGC 1 cut(s) 69
BciVI GTATCC 1 cut(s) 205
BclI TGATCA 1 cut(s) 105
BcoDI GTCTC 5 cut(s) 123, 160, 288, 417, 491
BfaI CTAG 1 cut(s) 246
BfuI GTATCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 378
BpiI GAAGAC 2 cut(s) 67, 508
BplI GAGNNNNNCTC 2 cut(s) 416, 448
Bpu10I CCTNAGC 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 1 cut(s) 390
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMII CTCAG 3 cut(s) 105, 323, 414
BsiSI CCGG 1 cut(s) 458
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 5 cut(s) 123, 160, 288, 417, 491
BsmBI CGTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 372
Bso31I GGTCTC 1 cut(s) 491
Bsp143I GATC 3 cut(s) 34, 105, 216
BspCNI CTCAG 3 cut(s) 106, 322, 415
BspLI GGNNCC 1 cut(s) 378
BspPI GGATC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 491
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 3 cut(s) 34, 105, 216
Bst4CI ACNGT 1 cut(s) 264
Bst6I CTCTTC 2 cut(s) 143, 348
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 4 cut(s) 114, 309, 408, 423
BstDSI CCRYGG 1 cut(s) 390
BstKTI GATC 3 cut(s) 37, 108, 219
BstMAI GTCTC 5 cut(s) 123, 160, 288, 417, 491
BstMBI GATC 3 cut(s) 34, 105, 216
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstV2I GAAGAC 2 cut(s) 67, 508
BsuI GTATCC 1 cut(s) 205
BtgI CCRYGG 1 cut(s) 390
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 82
Csp6I GTAC 2 cut(s) 96, 260
CviAII CATG 1 cut(s) 66
CviJI RGCY 7 cut(s) 12, 25, 240, 395, 422, 428, 454
CviKI_1 RGCY 7 cut(s) 12, 25, 240, 395, 422, 428, 454
CviQI GTAC 2 cut(s) 96, 260
DdeI CTNAG 4 cut(s) 114, 309, 408, 423
DpnI GATC 3 cut(s) 36, 107, 218
DpnII GATC 3 cut(s) 34, 105, 216
Eam1104I CTCTTC 2 cut(s) 143, 348
EarI CTCTTC 2 cut(s) 143, 348
Eco31I GGTCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 281
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 1 cut(s) 69
FaiI YATR 8 cut(s) 28, 67, 131, 316, 323, 419, 471, 476
FaqI GGGAC 1 cut(s) 372
FatI CATG 1 cut(s) 65
FbaI TGATCA 1 cut(s) 105
FspBI CTAG 1 cut(s) 246
HapII CCGG 1 cut(s) 458
Hin1II CATG 1 cut(s) 69
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 9 cut(s) 116, 158, 168, 173, 277, 307, 335, 368, 545
HpaII CCGG 1 cut(s) 458
HphI GGTGA 2 cut(s) 362, 472
Hpy166II GTNNAC 2 cut(s) 125, 343
Hpy188I TCNGA 2 cut(s) 7, 276
Hpy188III TCNNGA 4 cut(s) 214, 398, 509, 549
Hpy8I GTNNAC 2 cut(s) 125, 343
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 264
HpyCH4IV ACGT 2 cut(s) 72, 258
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 4 cut(s) 114, 309, 408, 423
HpySE526I ACGT 2 cut(s) 72, 258
Hsp92II CATG 1 cut(s) 69
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 3 cut(s) 34, 105, 216
LpnPI CCDG 3 cut(s) 51, 471, 516
MaeI CTAG 1 cut(s) 246
MaeII ACGT 2 cut(s) 72, 258
MaeIII GTNAC 1 cut(s) 533
MalI GATC 3 cut(s) 36, 107, 218
MboI GATC 3 cut(s) 34, 105, 216
MboII GAAGA 7 cut(s) 67, 130, 145, 287, 340, 365, 508
MluCI AATT 1 cut(s) 101
MlyI GAGTC 4 cut(s) 125, 162, 271, 301
MmeI TCCRAC 1 cut(s) 299
MnlI CCTC 3 cut(s) 157, 418, 537
MroXI GAANNNNTTC 1 cut(s) 74
MspI CCGG 1 cut(s) 458
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 3 cut(s) 34, 105, 216
NlaIII CATG 1 cut(s) 69
NlaIV GGNNCC 1 cut(s) 378
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 4 cut(s) 158, 173, 335, 368
PflFI GACNNNGTC 1 cut(s) 280
PleI GAGTC 4 cut(s) 124, 162, 271, 301
PpsI GAGTC 4 cut(s) 124, 162, 271, 301
PspN4I GGNNCC 1 cut(s) 378
PsyI GACNNNGTC 1 cut(s) 280
RsaI GTAC 2 cut(s) 97, 261
RsaNI GTAC 2 cut(s) 96, 260
Sau3AI GATC 3 cut(s) 34, 105, 216
SchI GAGTC 4 cut(s) 125, 162, 271, 301
SetI ASST 6 cut(s) 27, 75, 149, 242, 261, 456
Sse9I AATT 1 cut(s) 101
SspMI CTAG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 264
TaiI ACGT 2 cut(s) 75, 261
TaqI TCGA 6 cut(s) 21, 33, 45, 156, 171, 215
TasI AATT 1 cut(s) 101
TfiI GAWTC 4 cut(s) 158, 173, 335, 368
TscAI CASTG 1 cut(s) 118
TspGWI ACGGA 3 cut(s) 166, 270, 369
TspRI CASTG 1 cut(s) 118
Tth111I GACNNNGTC 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.