MD09G1037200.v1.1
ERF Family

2'-5' RNA ligase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004409
Physical Location & Seq
Forward (+)
2273093 .. 2274039
947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1037200.v1.1.491

Sequence Viewer

Length: 552 bp
ATGTCACAGGGCTATGCAATCGAGCTTTATTTCGATCCAGCCCTCGAAAACCAGGTCTTGAAGGCTTGGAACGTGCTCGCCCGCCGTCAGATTAGTACCCAGCTCATCGAAATCGAATCACGACCGCACATCACGCTCTTCTCAAGCCCCTTCGTCGAACCCGCGAAGCTCGAAAACGTGATCAGAGCCTTCGCCTCCAAGCAAGAGCCTCTGGCCTTGTCCTTTTCTTCGATTGGGAGCCTCCCAAATGACAACAACGTCCTGTTTCTGTCGCCAACTCCGTCGGTTTCGTTGCTGCAGTTTCAGTCTCAATTGTGCGAGATAATGAAGAAGGAAGGTGTTGAAATTGGGGAGGAGTATCGCACTGACTGTTGGCTTCCTTACTGTGCTGTTGCTCAGGAAGTGCCCAAGACTCGAATGGCGGAAGCCTTTTGCGTGTTGAGGGACTTGAAGTTGCCGGTTGCAGGGTATGCCATGGATATTGGATTGGTGGAGTTTTCGCCGGTTCGCGAGCTGTTCTCGTTTGTGCTAGGTAACACAGTGGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2_5_RNA_ligase2

Protein Analysis

184

Amino Acids

20.51

Weight (kDa)

4.8

Isoelectric Point (pI)

66.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2_5_RNA_ligase2 PF13563 40 - 163 4.6e-12 2'-5' RNA ligase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AccII CGCG 2 cut(s) 164, 510
AciI CCGC 4 cut(s) 82, 125, 162, 422
AclWI GGATC 1 cut(s) 29
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 61, 344, 451
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 4 cut(s) 25, 103, 169, 514
AluI AGCT 4 cut(s) 25, 103, 169, 514
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 1 cut(s) 29
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 295
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
BaeGI GKGCMC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 69
BciT130I CCWGG 1 cut(s) 53
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 1 cut(s) 530
BfmI CTRYAG 1 cut(s) 296
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 53
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 53
BplI GAGNNNNNCTC 2 cut(s) 503, 535
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 2 cut(s) 457, 502
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMII CTCAG 1 cut(s) 410
BseRI GAGGAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 1 cut(s) 282
BseYI CCCAGC 1 cut(s) 99
Bsh1236I CGCG 2 cut(s) 164, 510
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 2 cut(s) 458, 503
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 458
BsnI GGCC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 78, 408
Bsp143I GATC 2 cut(s) 34, 180
Bsp19I CCATGG 1 cut(s) 474
Bsp68I TCGCGA 1 cut(s) 510
BspACI CCGC 4 cut(s) 82, 125, 162, 422
BspANI GGCC 1 cut(s) 215
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 2 cut(s) 164, 510
BspLI GGNNCC 1 cut(s) 239
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 1 cut(s) 29
BspQI GCTCTTC 1 cut(s) 143
BsrFI RCCGGY 2 cut(s) 457, 502
BssAI RCCGGY 2 cut(s) 457, 502
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 2 cut(s) 34, 180
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 3 cut(s) 371, 386, 541
Bst6I CTCTTC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 78, 82, 512
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 1 cut(s) 474
BstFNI CGCG 2 cut(s) 164, 510
BstKTI GATC 2 cut(s) 37, 183
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 2 cut(s) 34, 180
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 2 cut(s) 133, 470
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstSFI CTRYAG 1 cut(s) 296
BstSLI GKGCMC 1 cut(s) 408
BstUI CGCG 2 cut(s) 164, 510
BstV1I GCAGC 1 cut(s) 282
BsuRI GGCC 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 363, 546
BtuMI TCGCGA 1 cut(s) 510
Cac8I GCNNGC 3 cut(s) 78, 82, 512
Cfr10I RCCGGY 2 cut(s) 457, 502
CsiI ACCWGGT 1 cut(s) 51
Csp6I GTAC 1 cut(s) 96
CviAII CATG 2 cut(s) 475, 549
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 1 cut(s) 396
DpnI GATC 2 cut(s) 36, 182
DpnII GATC 2 cut(s) 34, 180
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
EciI GGCGGA 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 2 cut(s) 478, 552
FaiI YATR 4 cut(s) 15, 471, 476, 550
FaqI GGGAC 1 cut(s) 458
FatI CATG 2 cut(s) 474, 548
FauI CCCGC 2 cut(s) 89, 169
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 296
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 1 cut(s) 530
GluI GCNGC 1 cut(s) 296
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 215
HapII CCGG 2 cut(s) 458, 503
Hin1II CATG 2 cut(s) 478, 552
HinfI GANTC 2 cut(s) 116, 412
HpaII CCGG 2 cut(s) 458, 503
Hpy188I TCNGA 2 cut(s) 90, 185
Hpy188III TCNNGA 4 cut(s) 58, 120, 398, 509
Hpy99I CGWCG 2 cut(s) 158, 286
HpyAV CCTTC 5 cut(s) 55, 160, 199, 325, 329
HpyCH4III ACNGT 3 cut(s) 371, 386, 541
HpyCH4IV ACGT 3 cut(s) 72, 177, 258
HpyCH4V TGCA 3 cut(s) 17, 298, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 470
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 3 cut(s) 72, 177, 258
Hsp92II CATG 2 cut(s) 478, 552
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 34, 180
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 1 cut(s) 237
Lsp1109I GCAGC 1 cut(s) 282
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 1 cut(s) 530
MaeII ACGT 3 cut(s) 72, 177, 258
MaeIII GTNAC 2 cut(s) 3, 533
MalI GATC 2 cut(s) 36, 182
MboI GATC 2 cut(s) 34, 180
MboII GAAGA 3 cut(s) 130, 219, 340
MfeI CAATTG 1 cut(s) 311
MhlI GDGCHC 2 cut(s) 78, 408
MluCI AATT 2 cut(s) 311, 345
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 6 cut(s) 53, 205, 219, 251, 346, 435
MspI CCGG 2 cut(s) 458, 503
MspR9I CCNGG 1 cut(s) 53
MunI CAATTG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 2 cut(s) 164, 510
MwoI GCNNNNNNNGC 2 cut(s) 133, 470
NcoI CCATGG 1 cut(s) 474
NdeII GATC 2 cut(s) 34, 180
NlaIII CATG 2 cut(s) 478, 552
NlaIV GGNNCC 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 3
NruI TCGCGA 1 cut(s) 510
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 300
RruI TCGCGA 1 cut(s) 510
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 143
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 2 cut(s) 34, 180
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 2 cut(s) 78, 408
SexAI ACCWGGT 1 cut(s) 51
SfcI CTRYAG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 2 cut(s) 311, 345
SsiI CCGC 4 cut(s) 82, 125, 162, 422
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 3 cut(s) 371, 386, 541
TaiI ACGT 3 cut(s) 75, 180, 261
TaqI TCGA 9 cut(s) 21, 33, 45, 108, 114, 156, 171, 230, 415
TasI AATT 2 cut(s) 311, 345
TfiI GAWTC 1 cut(s) 116
TscAI CASTG 2 cut(s) 370, 546
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 1 cut(s) 341
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 2 cut(s) 370, 546
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.